CSPI07G05400 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G05400
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Chaperone protein dnaJ 20
Location	Chr7: 4050912 .. 4051409 (+)
RNA-Seq Expression	CSPI07G05400
Synteny	CSPI07G05400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTGCTTCACCAAATTCCTCTTCAACTTCTCTTTCTTTATCCTTCCTCTCCAAGACCACCCCTCCTTCTCATCAGGCGGCAAGCCGTGGCCGCTTCTGCGTCTCATGTAAAGCTTCTGCAACTAGAGAGAGAGATTATTACAAGTTGCTTTCGGTGAGTGGTGGGTGCAATGCTAGTCCTGAAGAAATTAAGAAGGCATACAGAGCCATGGCTTTGCGATATCATCCTGATCTTGTTTGTGATCCTTTGCTCAAAGAACAATCAACTAGAATGTTTGTTCAGCTCAATGCAGCTTACAAGACTCTCTCTGATCCTGTTCTTAGAAGACAATACGATGACTCTCTTAATATGGGATTCAATACAAAAGGCTTTCGAGGAGATTCAGCTGTTTGGGAAAGACAGATTCTTGAGCTCAAACGTCGCTCCTCTCAACGCAAAGATCGGTCCGCTCCTGCTTCGTGGGCTGCAAGAATGCAAGCTCATAGTCGTTGTTAG

mRNA sequence

ATGTTTGCTTCACCAAATTCCTCTTCAACTTCTCTTTCTTTATCCTTCCTCTCCAAGACCACCCCTCCTTCTCATCAGGCGGCAAGCCGTGGCCGCTTCTGCGTCTCATGTAAAGCTTCTGCAACTAGAGAGAGAGATTATTACAAGTTGCTTTCGGTGAGTGGTGGGTGCAATGCTAGTCCTGAAGAAATTAAGAAGGCATACAGAGCCATGGCTTTGCGATATCATCCTGATCTTGTTTGTGATCCTTTGCTCAAAGAACAATCAACTAGAATGTTTGTTCAGCTCAATGCAGCTTACAAGACTCTCTCTGATCCTGTTCTTAGAAGACAATACGATGACTCTCTTAATATGGGATTCAATACAAAAGGCTTTCGAGGAGATTCAGCTGTTTGGGAAAGACAGATTCTTGAGCTCAAACGTCGCTCCTCTCAACGCAAAGATCGGTCCGCTCCTGCTTCGTGGGCTGCAAGAATGCAAGCTCATAGTCGTTGTTAG

Coding sequence (CDS)

ATGTTTGCTTCACCAAATTCCTCTTCAACTTCTCTTTCTTTATCCTTCCTCTCCAAGACCACCCCTCCTTCTCATCAGGCGGCAAGCCGTGGCCGCTTCTGCGTCTCATGTAAAGCTTCTGCAACTAGAGAGAGAGATTATTACAAGTTGCTTTCGGTGAGTGGTGGGTGCAATGCTAGTCCTGAAGAAATTAAGAAGGCATACAGAGCCATGGCTTTGCGATATCATCCTGATCTTGTTTGTGATCCTTTGCTCAAAGAACAATCAACTAGAATGTTTGTTCAGCTCAATGCAGCTTACAAGACTCTCTCTGATCCTGTTCTTAGAAGACAATACGATGACTCTCTTAATATGGGATTCAATACAAAAGGCTTTCGAGGAGATTCAGCTGTTTGGGAAAGACAGATTCTTGAGCTCAAACGTCGCTCCTCTCAACGCAAAGATCGGTCCGCTCCTGCTTCGTGGGCTGCAAGAATGCAAGCTCATAGTCGTTGTTAG

Protein sequence

MFASPNSSSTSLSLSFLSKTTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNMGFNTKGFRGDSAVWERQILELKRRSSQRKDRSAPASWAARMQAHSRC*

Homology

[Close]

BLAST of CSPI07G05400 vs. ExPASy Swiss-Prot
Match: Q3AF07 (Chaperone protein DnaJ OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 6.0e-11
Identity = 38/93 (40.86%), Postives = 55/93 (59.14%), Query Frame = 0


Query: 44  ERDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTL 103
           +RDYY++L V+   NA+PEEIKKAYR +A +YHPD+  D      +   F ++N AY+ L
Sbjct: 2   KRDYYEILGVAR--NATPEEIKKAYRKLARKYHPDVNKD---DPNAAEKFKEINEAYEVL 61

Query: 104 SDPVLRRQYDDSLNMGFNTK-----GFRGDSAV 132
           SDP  R +YD   + G +       GF G + +
Sbjct: 62  SDPEKRARYDQFGHAGVDGNFAGQGGFGGGAGI 89

[Close]

BLAST of CSPI07G05400 vs. ExPASy Swiss-Prot
Match: Q5U458 (DnaJ homolog subfamily C member 11 OS=Mus musculus OX=10090 GN=Dnajc11 PE=1 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 1.7e-10
Identity = 41/103 (39.81%), Postives = 57/103 (55.34%), Query Frame = 0


Query: 46  DYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSD 105
           DYY LL+V     AS EE+K AYR + + YHPD   DP LK Q+ R+F  ++ AY+ LSD
Sbjct: 14  DYYSLLNVRR--EASSEELKAAYRRLCMLYHPDKHRDPELKSQAERLFNLVHQAYEVLSD 73

Query: 106 PVLRRQYDDSLNMGFNTKGFRGDSAVWERQILELKRRSSQRKD 149
           P  R  YD     G   +G       WE  ++E KR  ++ ++
Sbjct: 74  PQTRAIYDIYGKRGLEMEG-------WE--VVERKRTPAEIRE 105

[Close]

BLAST of CSPI07G05400 vs. ExPASy Swiss-Prot
Match: Q9UXR9 (Chaperone protein DnaJ OS=Methanosarcina thermophila OX=2210 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.3e-10
Identity = 35/77 (45.45%), Postives = 51/77 (66.23%), Query Frame = 0


Query: 45  RDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLS 104
           RDYY++L +S   +A+PE+IKK+YR +AL+YHPD   +P  +E+    F +++ AY  LS
Sbjct: 5   RDYYEILGLSR--DATPEDIKKSYRKLALKYHPDRNKEPGAEEK----FKEISEAYAVLS 64

Query: 105 DPVLRRQYDDSLNMGFN 122
           DP  R QYD   + G N
Sbjct: 65  DPEKRAQYDRFGHAGIN 75

[Close]

BLAST of CSPI07G05400 vs. ExPASy Swiss-Prot
Match: Q9SDN0 (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 2.3e-10
Identity = 58/173 (33.53%), Postives = 91/173 (52.60%), Query Frame = 0


Query: 3   ASPNSSSTSLSLSFLSKTTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGCNASPE 62
           +S   +S   ++S+ ++T   S +  SR       K S   +  +Y LL V+   + +  
Sbjct: 25  SSLQPTSIPTTISYPTRTRFSSTRIQSRLTHDDPVKQS--EDLSFYDLLGVTE--SVTLP 84

Query: 63  EIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNMG--F 122
           EIK+AY+ +A +YHPD V  P   E+ T  F+++  AY+TLSDP  R  YD  L+MG  F
Sbjct: 85  EIKQAYKQLARKYHPD-VSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSF 144

Query: 123 NTKGFRGD-------------SAVWERQILELKRRSSQRKDRSAPASWAARMQ 161
           +  G R +              A W+ Q+  L+RRS+Q+ + +   SWAARM+
Sbjct: 145 SFSGRRQNRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQKDNNT--MSWAARMR 190

[Close]

BLAST of CSPI07G05400 vs. ExPASy Swiss-Prot
Match: Q9NVH1 (DnaJ homolog subfamily C member 11 OS=Homo sapiens OX=9606 GN=DNAJC11 PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 3.0e-10
Identity = 41/107 (38.32%), Postives = 56/107 (52.34%), Query Frame = 0


Query: 46  DYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSD 105
           DYY LL+V     AS EE+K AYR + + YHPD   DP LK Q+ R+F  ++ AY+ LSD
Sbjct: 14  DYYSLLNVRR--EASSEELKAAYRRLCMLYHPDKHRDPELKSQAERLFNLVHQAYEVLSD 73

Query: 106 PVLRRQYDDSLNMGFNTKGF-----RGDSAVWERQILELKRRSSQRK 148
           P  R  YD     G   +G+     R   A    +   L+R   +R+
Sbjct: 74  PQTRAIYDIYGKRGLEMEGWEVVERRRTPAEIREEFERLQREREERR 118

[Close]

BLAST of CSPI07G05400 vs. ExPASy TrEMBL
Match: A0A0A0K7A4 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G061190 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 2.6e-86
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0


Query: 1   MFASPNSSSTSLSLSFLSKTTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGCNAS 60
           MFASPNSSSTSLSLSFLSKTTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGCNAS
Sbjct: 1   MFASPNSSSTSLSLSFLSKTTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGCNAS 60

Query: 61  PEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNMGF 120
           PEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNMGF
Sbjct: 61  PEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNMGF 120

Query: 121 NTKGFRGDSAVWERQILELKRRSSQRKDRSAPASWAARMQAHSRC 166
           NTKGFRGDSAVWERQILELKRRSSQRKDRSAPASWAARMQAHSRC
Sbjct: 121 NTKGFRGDSAVWERQILELKRRSSQRKDRSAPASWAARMQAHSRC 165

[Close]

BLAST of CSPI07G05400 vs. ExPASy TrEMBL
Match: A0A5A7SN90 (Chaperone protein dnaJ 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002620 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 8.0e-67
Identity = 147/168 (87.50%), Postives = 151/168 (89.88%), Query Frame = 0


Query: 1   MFASPNSSSTSLSLSFLSKTTPPSHQAASRGRFCVSCKASA-TRERD-YYKLLSVSGGCN 60
           MFASPN    SLSLSF SK TPPS Q ASRGR CVSCKA+A T+ERD YYKLLSVSGGCN
Sbjct: 1   MFASPN----SLSLSFPSKATPPSFQ-ASRGRCCVSCKAAASTKERDNYYKLLSVSGGCN 60

Query: 61  ASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNM 120
           ASPEEIKKAYRAMALRYHPDLVCDP LKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSL M
Sbjct: 61  ASPEEIKKAYRAMALRYHPDLVCDPSLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSL-M 120

Query: 121 GFNTKGFRGDS-AVWERQILELKRRSSQRKDRSAPASWAARMQAHSRC 166
           GFNTK F+GDS AVW+RQILELKRRSS RKDRSAPASWAARMQA SRC
Sbjct: 121 GFNTKRFQGDSAAVWQRQILELKRRSSLRKDRSAPASWAARMQAQSRC 162

[Close]

BLAST of CSPI07G05400 vs. ExPASy TrEMBL
Match: A0A1S3C0P6 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103495683 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 8.0e-67
Identity = 147/168 (87.50%), Postives = 151/168 (89.88%), Query Frame = 0


Query: 1   MFASPNSSSTSLSLSFLSKTTPPSHQAASRGRFCVSCKASA-TRERD-YYKLLSVSGGCN 60
           MFASPN    SLSLSF SK TPPS Q ASRGR CVSCKA+A T+ERD YYKLLSVSGGCN
Sbjct: 1   MFASPN----SLSLSFPSKATPPSFQ-ASRGRCCVSCKAAASTKERDNYYKLLSVSGGCN 60

Query: 61  ASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNM 120
           ASPEEIKKAYRAMALRYHPDLVCDP LKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSL M
Sbjct: 61  ASPEEIKKAYRAMALRYHPDLVCDPSLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSL-M 120

Query: 121 GFNTKGFRGDS-AVWERQILELKRRSSQRKDRSAPASWAARMQAHSRC 166
           GFNTK F+GDS AVW+RQILELKRRSS RKDRSAPASWAARMQA SRC
Sbjct: 121 GFNTKRFQGDSAAVWQRQILELKRRSSLRKDRSAPASWAARMQAQSRC 162

[Close]

BLAST of CSPI07G05400 vs. ExPASy TrEMBL
Match: A0A6J1HXC8 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111467103 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.6e-43
Identity = 98/138 (71.01%), Postives = 113/138 (81.88%), Query Frame = 0


Query: 35  VSCKASATRERDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFV 94
           +SCKA+ T+  DYYKLLSVS GCNAS EEIK+AYRAMAL+YHPDLVCDPLLKEQST+MFV
Sbjct: 12  LSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFV 71

Query: 95  QLNAAYKTLSDPVLRRQYDDS---LNMGFN--TKGFRGDSAVWERQILELKRRSSQRKDR 154
           QLNAAYKTLSDPVLRRQYDDS   LN G++   +GF  D  VW+RQILELKRRS+ ++DR
Sbjct: 72  QLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERRGFEVDRTVWQRQILELKRRSACQRDR 131

Query: 155 SAPASWAARMQA--HSRC 166
           S   SW  RM+A  +S C
Sbjct: 132 S--VSWGGRMRARNYSEC 147

[Close]

BLAST of CSPI07G05400 vs. ExPASy TrEMBL
Match: A0A6J1HH09 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111463967 PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.8e-43
Identity = 98/138 (71.01%), Postives = 113/138 (81.88%), Query Frame = 0


Query: 35  VSCKASATRERDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFV 94
           +SCKA+ T+  DYYKLLSVS GCNAS EEIK+AYRAMAL+YHPDLVCDPLLKEQST+MFV
Sbjct: 12  LSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFV 71

Query: 95  QLNAAYKTLSDPVLRRQYDDS---LNMGFNTK--GFRGDSAVWERQILELKRRSSQRKDR 154
           QLNAAYKTLSDPVLRRQYDDS   LN G++ +  GF  D  VW+RQILELKRRS+ ++DR
Sbjct: 72  QLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILELKRRSACQRDR 131

Query: 155 SAPASWAARMQA--HSRC 166
           S   SW  RM+A  +S C
Sbjct: 132 S--VSWGGRMRARNYSEC 147

[Close]

BLAST of CSPI07G05400 vs. NCBI nr
Match: XP_004136866.1 (chaperone protein dnaJ 20, chloroplastic [Cucumis sativus])

HSP 1 Score: 327.8 bits (839), Expect = 5.5e-86
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0


Query: 1   MFASPNSSSTSLSLSFLSKTTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGCNAS 60
           MFASPNSSSTSLSLSFLSKTTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGCNAS
Sbjct: 1   MFASPNSSSTSLSLSFLSKTTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGCNAS 60

Query: 61  PEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNMGF 120
           PEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNMGF
Sbjct: 61  PEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNMGF 120

Query: 121 NTKGFRGDSAVWERQILELKRRSSQRKDRSAPASWAARMQAHSRC 166
           NTKGFRGDSAVWERQILELKRRSSQRKDRSAPASWAARMQAHSRC
Sbjct: 121 NTKGFRGDSAVWERQILELKRRSSQRKDRSAPASWAARMQAHSRC 165

[Close]

BLAST of CSPI07G05400 vs. NCBI nr
Match: KAE8645798.1 (hypothetical protein Csa_017276 [Cucumis sativus])

HSP 1 Score: 299.7 bits (766), Expect = 1.6e-77
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 19  KTTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPD 78
           KTTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPD
Sbjct: 7   KTTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPD 66

Query: 79  LVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNMGFNTKGFRGDSAVWERQILE 138
           LVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNMGFNTKGFRGDSAVWERQILE
Sbjct: 67  LVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNMGFNTKGFRGDSAVWERQILE 126

Query: 139 LKRRSSQRKDRSAPASWAARMQAHSRC 166
           LKRRSSQRKDRSAPASWAARMQAHSRC
Sbjct: 127 LKRRSSQRKDRSAPASWAARMQAHSRC 153

[Close]

BLAST of CSPI07G05400 vs. NCBI nr
Match: XP_008455532.1 (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo] >KAA0031496.1 chaperone protein dnaJ 20 [Cucumis melo var. makuwa] >TYK06949.1 chaperone protein dnaJ 20 [Cucumis melo var. makuwa])

HSP 1 Score: 263.1 bits (671), Expect = 1.7e-66
Identity = 147/168 (87.50%), Postives = 151/168 (89.88%), Query Frame = 0


Query: 1   MFASPNSSSTSLSLSFLSKTTPPSHQAASRGRFCVSCKASA-TRERD-YYKLLSVSGGCN 60
           MFASPN    SLSLSF SK TPPS Q ASRGR CVSCKA+A T+ERD YYKLLSVSGGCN
Sbjct: 1   MFASPN----SLSLSFPSKATPPSFQ-ASRGRCCVSCKAAASTKERDNYYKLLSVSGGCN 60

Query: 61  ASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNM 120
           ASPEEIKKAYRAMALRYHPDLVCDP LKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSL M
Sbjct: 61  ASPEEIKKAYRAMALRYHPDLVCDPSLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSL-M 120

Query: 121 GFNTKGFRGDS-AVWERQILELKRRSSQRKDRSAPASWAARMQAHSRC 166
           GFNTK F+GDS AVW+RQILELKRRSS RKDRSAPASWAARMQA SRC
Sbjct: 121 GFNTKRFQGDSAAVWQRQILELKRRSSLRKDRSAPASWAARMQAQSRC 162

[Close]

BLAST of CSPI07G05400 vs. NCBI nr
Match: XP_038888723.1 (dnaJ homolog subfamily A member 3, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 221.5 bits (563), Expect = 5.5e-54
Identity = 123/173 (71.10%), Postives = 137/173 (79.19%), Query Frame = 0


Query: 1   MFASPNSSSTSLSLSFLSK---TTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGC 60
           MFASPNSSS SL+L F SK   T PP+  AA R     +  A+A +ERDYYKLLSVSGGC
Sbjct: 1   MFASPNSSSNSLALFFPSKTITTAPPTAMAACRFSCKAAAVAAAPKERDYYKLLSVSGGC 60

Query: 61  NASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLN 120
           NAS EEIKKAYRAMAL+YHPDLVCDPLLKEQSTR+FVQLNAAYKTLSDPVLRRQYD SL 
Sbjct: 61  NASNEEIKKAYRAMALQYHPDLVCDPLLKEQSTRIFVQLNAAYKTLSDPVLRRQYDASL- 120

Query: 121 MGFNT-------KGFRGDSAVWERQILELKRRSSQRKDRSAPASWAARMQAHS 164
           MGFN        +GF+ D+ VW+RQ+LELKRRS+ R+DRSA ASW A MQA S
Sbjct: 121 MGFNNNGFSNGRRGFQADTTVWQRQLLELKRRSALRRDRSAKASWGATMQARS 172

[Close]

BLAST of CSPI07G05400 vs. NCBI nr
Match: XP_022967659.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 185.7 bits (470), Expect = 3.3e-43
Identity = 98/138 (71.01%), Postives = 113/138 (81.88%), Query Frame = 0


Query: 35  VSCKASATRERDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFV 94
           +SCKA+ T+  DYYKLLSVS GCNAS EEIK+AYRAMAL+YHPDLVCDPLLKEQST+MFV
Sbjct: 12  LSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFV 71

Query: 95  QLNAAYKTLSDPVLRRQYDDS---LNMGFN--TKGFRGDSAVWERQILELKRRSSQRKDR 154
           QLNAAYKTLSDPVLRRQYDDS   LN G++   +GF  D  VW+RQILELKRRS+ ++DR
Sbjct: 72  QLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERRGFEVDRTVWQRQILELKRRSACQRDR 131

Query: 155 SAPASWAARMQA--HSRC 166
           S   SW  RM+A  +S C
Sbjct: 132 S--VSWGGRMRARNYSEC 147

[Close]

BLAST of CSPI07G05400 vs. TAIR 10
Match: AT4G13830.2 (DNAJ-like 20 )

HSP 1 Score: 67.0 bits (162), Expect = 1.6e-11
Identity = 58/173 (33.53%), Postives = 91/173 (52.60%), Query Frame = 0


Query: 3   ASPNSSSTSLSLSFLSKTTPPSHQAASRGRFCVSCKASATRERDYYKLLSVSGGCNASPE 62
           +S   +S   ++S+ ++T   S +  SR       K S   +  +Y LL V+   + +  
Sbjct: 25  SSLQPTSIPTTISYPTRTRFSSTRIQSRLTHDDPVKQS--EDLSFYDLLGVTE--SVTLP 84

Query: 63  EIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSDPVLRRQYDDSLNMG--F 122
           EIK+AY+ +A +YHPD V  P   E+ T  F+++  AY+TLSDP  R  YD  L+MG  F
Sbjct: 85  EIKQAYKQLARKYHPD-VSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSF 144

Query: 123 NTKGFRGD-------------SAVWERQILELKRRSSQRKDRSAPASWAARMQ 161
           +  G R +              A W+ Q+  L+RRS+Q+ + +   SWAARM+
Sbjct: 145 SFSGRRQNRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQKDNNT--MSWAARMR 190

[Close]

BLAST of CSPI07G05400 vs. TAIR 10
Match: AT2G35720.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 61.6 bits (148), Expect = 6.8e-10
Identity = 39/107 (36.45%), Postives = 57/107 (53.27%), Query Frame = 0


Query: 45  RDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLS 104
           R+ Y LL++S    AS EEI+KAYR  A  YHPD +  P +KE +T  F ++  AY+ LS
Sbjct: 14  RELYALLNLSP--EASDEEIRKAYRQWAQVYHPDKIQSPQMKEVATENFQRICEAYEILS 73

Query: 105 DPVLRRQYD----DSLNMGFNTKGFRGDSAVWERQILELKRRSSQRK 148
           D   R  YD    + LN G         +   + ++  +KRR+ + K
Sbjct: 74  DETKRLIYDLYGMEGLNSGLELGPRLSKADEIKEELERIKRRNEEAK 118

[Close]

BLAST of CSPI07G05400 vs. TAIR 10
Match: AT2G20560.1 (DNAJ heat shock family protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.2e-09
Identity = 32/83 (38.55%), Postives = 52/83 (62.65%), Query Frame = 0


Query: 46  DYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSD 105
           DYYK+L V    +AS +++KKAYR +A+++HPD   +P  K+ +  MF Q++ AY+ LSD
Sbjct: 4   DYYKVLQVDR--SASDDDLKKAYRKLAMKWHPDK--NPNNKKDAEAMFKQISEAYEVLSD 63

Query: 106 PVLRRQYDDSLNMGFNTKGFRGD 129
           P  +  YD      +  +G +G+
Sbjct: 64  PQKKAVYDQ-----YGEEGLKGN 77

[Close]

BLAST of CSPI07G05400 vs. TAIR 10
Match: AT1G10350.1 (DNAJ heat shock family protein )

HSP 1 Score: 60.1 bits (144), Expect = 2.0e-09
Identity = 29/68 (42.65%), Postives = 46/68 (67.65%), Query Frame = 0


Query: 46  DYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLSD 105
           DYY +L V+   NA+ +++KK+YR MA+++HPD   +P  K+++   F Q++ AY  LSD
Sbjct: 4   DYYNVLKVNR--NANEDDLKKSYRRMAMKWHPDK--NPTSKKEAEAKFKQISEAYDVLSD 63

Query: 106 PVLRRQYD 114
           P  R+ YD
Sbjct: 64  PQRRQIYD 67

[Close]

BLAST of CSPI07G05400 vs. TAIR 10
Match: AT2G33735.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 2.0e-09
Identity = 26/69 (37.68%), Postives = 49/69 (71.01%), Query Frame = 0


Query: 45  RDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFVQLNAAYKTLS 104
           +D+YK+L ++  C+AS +EI+ ++  +AL++HPD   +   ++ +T  F ++N AY+ LS
Sbjct: 21  KDHYKVLELN--CDASDDEIRSSFIRLALKWHPDKFKE---EDSATSRFQEINEAYQVLS 80

Query: 105 DPVLRRQYD 114
           DP+ R++YD
Sbjct: 81  DPIARQEYD 84

The following BLAST results are available for this feature:

BLAST of CSPI07G05400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3AF07	6.0e-11	40.86	Chaperone protein DnaJ OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161...	[more]
Q5U458	1.7e-10	39.81	DnaJ homolog subfamily C member 11 OS=Mus musculus OX=10090 GN=Dnajc11 PE=1 SV=2	[more]
Q9UXR9	2.3e-10	45.45	Chaperone protein DnaJ OS=Methanosarcina thermophila OX=2210 GN=dnaJ PE=3 SV=1	[more]
Q9SDN0	2.3e-10	33.53	Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ2...	[more]
Q9NVH1	3.0e-10	38.32	DnaJ homolog subfamily C member 11 OS=Homo sapiens OX=9606 GN=DNAJC11 PE=1 SV=2	[more]

BLAST of CSPI07G05400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K7A4	2.6e-86	100.00	J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G061190 PE=4 SV=1	[more]
A0A5A7SN90	8.0e-67	87.50	Chaperone protein dnaJ 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3C0P6	8.0e-67	87.50	chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1HXC8	1.6e-43	71.01	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1HH09	2.8e-43	71.01	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of CSPI07G05400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136866.1	5.5e-86	100.00	chaperone protein dnaJ 20, chloroplastic [Cucumis sativus]	[more]
KAE8645798.1	1.6e-77	100.00	hypothetical protein Csa_017276 [Cucumis sativus]	[more]
XP_008455532.1	1.7e-66	87.50	PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo] >KAA0031...	[more]
XP_038888723.1	5.5e-54	71.10	dnaJ homolog subfamily A member 3, mitochondrial-like [Benincasa hispida]	[more]
XP_022967659.1	3.3e-43	71.01	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of CSPI07G05400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13830.2	1.6e-11	33.53	DNAJ-like 20	[more]
AT2G35720.1	6.8e-10	36.45	DNAJ heat shock N-terminal domain-containing protein	[more]
AT2G20560.1	1.2e-09	38.55	DNAJ heat shock family protein	[more]
AT1G10350.1	2.0e-09	42.65	DNAJ heat shock family protein	[more]
AT2G33735.1	2.0e-09	37.68	Chaperone DnaJ-domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 50..68 score: 35.8 coord: 68..83 score: 43.64 coord: 88..108 score: 31.16
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 45..108 e-value: 1.9E-18 score: 77.3
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 46..113 e-value: 2.3E-18 score: 66.1
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 46..116 score: 17.95348
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 46..105 e-value: 4.07222E-18 score: 71.4225
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 34..131 e-value: 1.7E-23 score: 84.8
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 43..134
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 143..165
None	No IPR available	PANTHER	PTHR45090	CHAPERONE PROTEIN DNAJ 20 CHLOROPLASTIC	coord: 6..163
None	No IPR available	PANTHER	PTHR45090:SF8	CHAPERONE DNAJ DOMAIN PROTEIN	coord: 6..163

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G05400.1	CSPI07G05400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061077	chaperone-mediated protein folding
cellular_component	GO:0009507	chloroplast