CSPI07G02590 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G02590
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRibulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase
LocationChr7: 2196560 .. 2201933 (+)
RNA-Seq ExpressionCSPI07G02590
SyntenyCSPI07G02590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAACGATCAACGTCTTTTTATCGACTTCTCTCTTCAGATAAGGTTTATTTTCTATCTCTCTCGTCTTCGAGCTCAGCCATGGCTGAAGCTTCAAGGGTTTTCAATTCTTCCCTTCTTCCCAACTTTCGTCCACTTCAAAACACTCTTTCAACCAAGCCCCACACTGCAACTTTTCGCAGACACTCAATCAACTGCTCCGTCTCCACCACTGACAGTGCCAGAGTGGCAGCCACCGGCCCCATTCCATGGGGTTGCGAAATCGATTCGTTGGAGAACGCTTCTGCTCTTCAGAAATGGTTGTCGGAGTCTGGCTTACCTGACCAGAAGATGTCGATTCAGAGAGTGAATGTTGGAGAGAGAGGGCTTGTGGCTTTGAAGAATGTTAGGAAAGGGGAGAAGTTACTGTTTGTGCCGCCTTCTCTCGTTATCTCTGCAGAATCGGTTAGTTTTCTTCATTGGGAGTTCGTAAAATGGCAGAATTTTGCACCAATTGTTATTTAACGGTACTCTTGTGGGAACGTTTTAGTTGTCTGCAAACTGTTCGGTGAATTTTGTTTAAGTCGTTTTCATTGCGGTACCTGATTATGGTGTTGGATTGTATAGCGACCAATCGTGTCTAAGTTTAGCTTCTTTTTATTTATTATTATTATCTTTTTTTTAAAGATATGAGTAGTTGTGTTTGAATGGAAAGTGGGCAGATGAAGTCAATCCTCCTCTTTCAAGGAGCAACAGAGGAGCAAAATTAGAAATTTGCACTGGCAAACCATTTTTTTATGATTCCTTTCTTTTGTAAATTTCATACATCAATTAAAATTGTTTCTCATAAAAAGAAAAAGAAAGGCTAATTGCCCTTGGACTAACCACGTTGCAATTTTGGGATGGTTGGAGTAAACTACACGAATGGTTTTAAGTGAACTAGGCTTGTAATTTGACTTCTTTGGTGGGGAGTGTGCAAGCGTTGCCTTTGCAACTTAACTTCAAGGTTATGGTTTTTCATTATCATCTAAGCTTATAAACCAACAATATAGCCTTTTCCACCTTCTTCCAATAGGAAAACGAAAAATGCTACACCAGTAAATGCCTTCCCAATCTCAGCTTGTCTCTTTGTTTTCTATGTTAGGAGTGGAGCTGCCCTGAGGCTGGGGAGGTGTTAAAACGTAATTCAGTACCAGATTGGCCACTTATTGCAACCTACTTGATAAGTGAAGCAAGTCTAATGAAATCATCAAGATGGAACAACTATATATCAGCCTTACCTCGGCAACCTTATTCACTTTTGTACTGGTCAGTTGCTTTATGGTCAAGCAACTTTATCTTTTTGTGTGCCTGGACTTTATACCTAGTCCTCACTGATATAGCTTCTCTTTTATCATTTCAGGACACGTGAAGAGCTAGATCGATATCTAGAAGCATCAGAGATCAGAGAAAGGGCAATAGAAAGGATCACAAATGTTGTAGGAACGTAAGGCCACATTTTCTCTTTGCTTATTTTCCTATTAAGTTTAGTTAAACTGCTATGAATGCTAGAATTTTCAATCAGTAGCCTAAATTCTCGTAGTACATCATTCTGTTGTTAATTAAATAATAATTTGTACTCTGTGATTTCTGGTTCAGATACAATGATTTAAGCATCAGGGTATTTTCTAAACATCCCGAATTATTCCCCGAGGAGGTATTAAGTTCTATGTTCTCCAAGAAGTTTTCGCACTATCATTTTATAACTTTGGTTTATTCACACTGGAATATGTTCAACCTCTTTTTGTCCAACTGGTGTCAACTCTGCTAAAACACCATTGTGTTCATCTATTTTTTTGGAACGGAGATGAAACCTCTCTATTTTTTTAAAACCATTGTGTTCATCTATTCAGTTAGGTTTTGGCATTGAAGAGAAAATGGTGTAAACCAAAATCTAAATTTGTATGAAATGACACTCTTCTTCAATTAGGGGAGGAGCTTTCTTCATAGTGGAAGTGATTACAGGAATGTGAGAGAATGTTATAACAATCAAACTAGCTAAAGATTCACTACCTAACTCTAAGCTAGTTAGAAAAATAAGGCCTCACACTACACCAGTAAACTTGTTTTCGGCTCAGGTTTGTCTATTAAAATATCTATTCTCTTGAGAAGAGTTTTGATCAACCAAAAACCAACAAGTTCTTGATCAACTTTAAGCACATATTTATTTTTAAGCGGAGACAAAACATTTTTTAAAGCACATTACAGTACAGTTCTTTTCAATTTAACATTTAGTTCCTGGGGGACCTGGTTTAGCCATTTAATAATTATGTCTACATTATTCAATTTCAATTGCTACATTTTAGGGAAAATTGCAAATTTATAGTTTTGGTTGCCATACAATTATGTACATAAATACGTTCCAGTCCTTGAGCAAACCCCAATTTACTCAACTCTTGTTTTCTTGAAAAATGAAAGATAACTATATTCTTCCTGGATATTTGATTTTCAGGTATTCAATATTGAAACTTTCAAGTGGTCGTTTGGGATTCTTTTCTCCCGCTTGGTAAGTATGTGGTATGCTTAATAATTGGAGATCAAGGCATAAAACTATGTTATAGGGATTAATTTGGAAACAACTTGGTTGCACTTCTGAAATGGTATTCATCTATTGAAACTGTAAAAGCCTTAACTTTTAGTTTATTTTATTTTTTAACATCTATCTTTGCTGCCCCTTTTCTTTATATATATTTTTTGTCCATATGTAATTTGAATGCTGACAAGATAACATTTCTCTCAAGCCCAGGTTCGGTTACCCTCTATGGATGGAAAGGTTGCATTGGTTCCCTGGGCTGATATGCTGAATCATAACTGTGAGGTACGCTGATTGGTATTTTTCTAAAGCCGTGAGTTGAGCTGCAGAATTGAAATTTAACCCTTGCAACTATGAATGCTGCCTCAGGTGGAGACTTTCTTGGATTATGATAAAGCATCCCAAGGTGTTGTATTTACAACAGATCGAGCATACCAACCAGGTGAGCAGGTAAAAGAACATTTAAAATTTTCATCATGGATGAATCTGATGGAATAATGTAAAACTCAAATTTCTTGGGAACTTGAATTAGAAAAATGAAACTATTTCGAACTTGCAAATCATATTGGATCTTGAGTCCATGATGAAACATAATGGCCAGTGCCATAAATGGTAGAAAGAAAGATCAGAAACTTTTTTTCACTTCACGCTTCCAAAAGGAACATTTCCCAGATAGACGAAATTTATGTCAACCACAAAAGTTGTATTACTATGCATCGTGTTTACTTCAATCACTGATCACTGCAGGTGTTCATTTCATATGGCAAGAAATCTAATGGGGAGTTGTTGTTGTCATATGGCTTTGTTCCAAAGGAGGGAAGCAATCCTAGCGATTCTGTTGAGTTGTTACTCTCCCTAAAAAAATCTGATAAATGTTATAAGGAGAAGTTGGAAGCACTGAAAAAGCATGGATTAAGGGCGTGAGTATGAAGAAATTGATGAGTTCTTCACCATTTATCGGGATTTAATTCAATGAACATTTTTATGACTTCCATTTTCCAAATTAAAAGAAGATATCATCTCATCTTCCTTCATAAAGATTCATCTTTAATTTCTGATGCAAATCTTAATAATTAGTGGTAAACTAATCACATTCGGGTTAGTTCCTCCCAACACTGTTTCTCTTGTTAATATCTTGTTATTTTGTTGATCGTGCTGGCTAAATTATCATGGCAGATCCCAGTGTTTTCCTATTCAAGTCACTGGCTGGCCTTTAGAATTGAAGGCATTTGCTTATTTAGCTGTTAGTCCACCAAGTTTGAGCAATCAGTTTGACGAGGTTTGGTTCCCTACCTCTTTTATCCTTATGTTCTTGAACTTCATTAAGCAATATCTTTCTCAATGTGTATCTGTGTATTGCTACCAGATGGCTGCCGCAGCATCTAATAAATCAACAGCAAAAAAGGATTTAAATTACCCTGATATAGAGGAGGAAGCGCTGCAGTTCATATTAGATAGCTGTGAAACTAGCATATCAAAATATAACAAATTTTTACAGGTAACGGATAAAGAATGTGCTGATTCTACTATTTTAGTGGACATTGACAACTTGAACTGAATGAACCTCTGATTCTTGCCATACTAGCTCATAATAGAAATCCATATTGATTTTGGAAGATTTTAGTGATACTGGAAATGCATATTTCTGACCTTATCTTTGCAGGCAAGTGGATCAATGGATTTGGATGTGACGTCGCCGAAACAACTCAACCGTAGAGTATTTTTGAAACAGTTGGCAGTAGACTTGTGCACTAGTGAACGAAGGATACTTTTTCGAAGCCAATATGTAAGCCCACGTGATCTCATATATCTCCAGCTTTCTTATTCTTCACGAAAATGACAAGCTTTTTGCATTTTCTGGTTGTTACCAAGACTATATTTGATAACTATTTGATTTTTTATGTTTTATTTTTTTAAAAAAACCTTACAAACACTATTTTTTTCACGTATAAGTTCCTTTGTTTCGTTATTTACCTTTGAGCATCGTTTCAAAAAAACCATATAAAAATTTGAAAACTAAGAAAGGTAGGCTCGTCGAAAGGTAGGCTTGTCTGATGATCATTTTGTTTTGAACATTATGCTTGTTTCTTTACAAATCTTGGTGAGTTTTTCACCTTTTTTCTTGTAAATACTTGTATTTTTGGAATTGGCTAAGAATTCAGAAAAATGGAAGACCGTAAAGAAAGAAATGGTGGGAAGACAAACACAATTTTCATAAACTAAATGTTTATCGAGCTAAGTTAAGTGGCTTGTGATTACTATTTTCTATGTAAATCTTAAAGGTTAGAACCTCTTATTTACTCTTTCTTCAATATTACTACTTCTACTAAAAAGAATCTGATAGCAATATTAAGAATTCGACTACTGTTATTGATATCTTGAGTATGGATGTTTTCAGATTCTGAGAAGAAGATTGAGAGACTTAAGAAGTGGTGAATTGAGAGCTCTAAAACTGTTCAGAGGGTTTGGGAAACTTTTCAAATGATTGGTTCACGAGTCGAACATGCTAGTCCTAAACCTTACAAAACACCCACCGGAGCTTTGCCCACTTGGCGTGGCCGCCACGGTTAGTTTCCTACTTGAGAATCAAAGAGTCTGGTCTATATAGCATCAAAGGTTCTGTTTAGAGTTGTGTAGTGAACAAAGTGAAAAAAGCAAAAGAACTTCCCCACTCGCTACCACTACCTCTTGATACATCTTCATGTTGTAAGTTACTAAGTTGTGTATTTTGTTGGACTAGTTTTAAACATTCTTGAGAGAAAAATAATGGATTTCAGTAGCTTTTGATTCAACACAATTTTCATTTCACTTTGTTATTTAGCTAC

mRNA sequence

GAAACGATCAACGTCTTTTTATCGACTTCTCTCTTCAGATAAGGTTTATTTTCTATCTCTCTCGTCTTCGAGCTCAGCCATGGCTGAAGCTTCAAGGGTTTTCAATTCTTCCCTTCTTCCCAACTTTCGTCCACTTCAAAACACTCTTTCAACCAAGCCCCACACTGCAACTTTTCGCAGACACTCAATCAACTGCTCCGTCTCCACCACTGACAGTGCCAGAGTGGCAGCCACCGGCCCCATTCCATGGGGTTGCGAAATCGATTCGTTGGAGAACGCTTCTGCTCTTCAGAAATGGTTGTCGGAGTCTGGCTTACCTGACCAGAAGATGTCGATTCAGAGAGTGAATGTTGGAGAGAGAGGGCTTGTGGCTTTGAAGAATGTTAGGAAAGGGGAGAAGTTACTGTTTGTGCCGCCTTCTCTCGTTATCTCTGCAGAATCGGAGTGGAGCTGCCCTGAGGCTGGGGAGGTGTTAAAACGTAATTCAGTACCAGATTGGCCACTTATTGCAACCTACTTGATAAGTGAAGCAAGTCTAATGAAATCATCAAGATGGAACAACTATATATCAGCCTTACCTCGGCAACCTTATTCACTTTTGTACTGGACACGTGAAGAGCTAGATCGATATCTAGAAGCATCAGAGATCAGAGAAAGGGCAATAGAAAGGATCACAAATGTTGTAGGAACATACAATGATTTAAGCATCAGGGTATTTTCTAAACATCCCGAATTATTCCCCGAGGAGGTATTCAATATTGAAACTTTCAAGTGGTCGTTTGGGATTCTTTTCTCCCGCTTGGTTCGGTTACCCTCTATGGATGGAAAGGTTGCATTGGTTCCCTGGGCTGATATGCTGAATCATAACTGTGAGGTGGAGACTTTCTTGGATTATGATAAAGCATCCCAAGGTGTTGTATTTACAACAGATCGAGCATACCAACCAGGTGAGCAGGTGTTCATTTCATATGGCAAGAAATCTAATGGGGAGTTGTTGTTGTCATATGGCTTTGTTCCAAAGGAGGGAAGCAATCCTAGCGATTCTGTTGAGTTGTTACTCTCCCTAAAAAAATCTGATAAATGTTATAAGGAGAAGTTGGAAGCACTGAAAAAGCATGGATTAAGGGCATCCCAGTGTTTTCCTATTCAAGTCACTGGCTGGCCTTTAGAATTGAAGGCATTTGCTTATTTAGCTGTTAGTCCACCAAGTTTGAGCAATCAGTTTGACGAGATGGCTGCCGCAGCATCTAATAAATCAACAGCAAAAAAGGATTTAAATTACCCTGATATAGAGGAGGAAGCGCTGCAGTTCATATTAGATAGCTGTGAAACTAGCATATCAAAATATAACAAATTTTTACAGGCAAGTGGATCAATGGATTTGGATGTGACGTCGCCGAAACAACTCAACCGTAGAGTATTTTTGAAACAGTTGGCAGTAGACTTGTGCACTAGTGAACGAAGGATACTTTTTCGAAGCCAATATATTCTGAGAAGAAGATTGAGAGACTTAAGAAGTGGTGAATTGAGAGCTCTAAAACTGTTCAGAGGGTTTGGGAAACTTTTCAAATGATTGGTTCACGAGTCGAACATGCTAGTCCTAAACCTTACAAAACACCCACCGGAGCTTTGCCCACTTGGCGTGGCCGCCACGGTTAGTTTCCTACTTGAGAATCAAAGAGTCTGGTCTATATAGCATCAAAGGTTCTGTTTAGAGTTGTGTAGTGAACAAAGTGAAAAAAGCAAAAGAACTTCCCCACTCGCTACCACTACCTCTTGATACATCTTCATGTTGTAAGTTACTAAGTTGTGTATTTTGTTGGACTAGTTTTAAACATTCTTGAGAGAAAAATAATGGATTTCAGTAGCTTTTGATTCAACACAATTTTCATTTCACTTTGTTATTTAGCTAC

Coding sequence (CDS)

ATGGCTGAAGCTTCAAGGGTTTTCAATTCTTCCCTTCTTCCCAACTTTCGTCCACTTCAAAACACTCTTTCAACCAAGCCCCACACTGCAACTTTTCGCAGACACTCAATCAACTGCTCCGTCTCCACCACTGACAGTGCCAGAGTGGCAGCCACCGGCCCCATTCCATGGGGTTGCGAAATCGATTCGTTGGAGAACGCTTCTGCTCTTCAGAAATGGTTGTCGGAGTCTGGCTTACCTGACCAGAAGATGTCGATTCAGAGAGTGAATGTTGGAGAGAGAGGGCTTGTGGCTTTGAAGAATGTTAGGAAAGGGGAGAAGTTACTGTTTGTGCCGCCTTCTCTCGTTATCTCTGCAGAATCGGAGTGGAGCTGCCCTGAGGCTGGGGAGGTGTTAAAACGTAATTCAGTACCAGATTGGCCACTTATTGCAACCTACTTGATAAGTGAAGCAAGTCTAATGAAATCATCAAGATGGAACAACTATATATCAGCCTTACCTCGGCAACCTTATTCACTTTTGTACTGGACACGTGAAGAGCTAGATCGATATCTAGAAGCATCAGAGATCAGAGAAAGGGCAATAGAAAGGATCACAAATGTTGTAGGAACATACAATGATTTAAGCATCAGGGTATTTTCTAAACATCCCGAATTATTCCCCGAGGAGGTATTCAATATTGAAACTTTCAAGTGGTCGTTTGGGATTCTTTTCTCCCGCTTGGTTCGGTTACCCTCTATGGATGGAAAGGTTGCATTGGTTCCCTGGGCTGATATGCTGAATCATAACTGTGAGGTGGAGACTTTCTTGGATTATGATAAAGCATCCCAAGGTGTTGTATTTACAACAGATCGAGCATACCAACCAGGTGAGCAGGTGTTCATTTCATATGGCAAGAAATCTAATGGGGAGTTGTTGTTGTCATATGGCTTTGTTCCAAAGGAGGGAAGCAATCCTAGCGATTCTGTTGAGTTGTTACTCTCCCTAAAAAAATCTGATAAATGTTATAAGGAGAAGTTGGAAGCACTGAAAAAGCATGGATTAAGGGCATCCCAGTGTTTTCCTATTCAAGTCACTGGCTGGCCTTTAGAATTGAAGGCATTTGCTTATTTAGCTGTTAGTCCACCAAGTTTGAGCAATCAGTTTGACGAGATGGCTGCCGCAGCATCTAATAAATCAACAGCAAAAAAGGATTTAAATTACCCTGATATAGAGGAGGAAGCGCTGCAGTTCATATTAGATAGCTGTGAAACTAGCATATCAAAATATAACAAATTTTTACAGGCAAGTGGATCAATGGATTTGGATGTGACGTCGCCGAAACAACTCAACCGTAGAGTATTTTTGAAACAGTTGGCAGTAGACTTGTGCACTAGTGAACGAAGGATACTTTTTCGAAGCCAATATATTCTGAGAAGAAGATTGAGAGACTTAAGAAGTGGTGAATTGAGAGCTCTAAAACTGTTCAGAGGGTTTGGGAAACTTTTCAAATGA

Protein sequence

MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDSARVAATGPIPWGCEIDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAESEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREELDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSRLVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKKSNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTGWPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSISKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRSGELRALKLFRGFGKLFK*
Homology
BLAST of CSPI07G02590 vs. ExPASy Swiss-Prot
Match: Q43088 (Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic OS=Pisum sativum OX=3888 GN=RBCMT PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.9e-24
Identity = 116/432 (26.85%), Postives = 203/432 (46.99%), Query Frame = 0

Query: 72  KWLSESGLPDQKMSIQRVNVGE-RGLVALKNVRKGEKLLFVPPSLVISAESEWSCPEAGE 131
           KWL E G+   K  ++   V E  GLVALK++ + + +L VP  L I+ ++  +  E G 
Sbjct: 59  KWLQEEGVITAKTPVKASVVTEGLGLVALKDISRNDVILQVPKRLWINPDAV-AASEIGR 118

Query: 132 VLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREELDRYLEASEI 191
           V   + +  W  +  +LI E S  + S W +Y   LP++  S +YW+ EEL   L+ S++
Sbjct: 119 VC--SELKPWLSVILFLIRERS-REDSVWKHYFGILPQETDSTIYWSEEELQE-LQGSQL 178

Query: 192 RERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR-LVRLPSMDG 251
            +  +     V      L   +   +  LFP+ V  ++ F W+FGIL SR   RL   + 
Sbjct: 179 LKTTVSVKEYVKNECLKLEQEIILPNKRLFPDPV-TLDDFFWAFGILRSRAFSRL--RNE 238

Query: 252 KVALVPWADMLNHNCEVET-FLDYDKASQGVVFTTDRAY--------QPGEQVFISYG-K 311
            + +VP AD++NH+  V T    Y+      +F+ D  +        + GEQV+I Y   
Sbjct: 239 NLVVVPMADLINHSAGVTTEDHAYEVKGAAGLFSWDYLFSLKSPLSVKAGEQVYIQYDLN 298

Query: 312 KSNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVT 371
           KSN EL L YGF+  E +    +  L L + +SD  + +KL+  + +G   +  F I   
Sbjct: 299 KSNAELALDYGFI--EPNENRHAYTLTLEISESDPFFDDKLDVAESNGFAQTAYFDIFYN 358

Query: 372 -----GWPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILD 431
                G    L+  A        L + F +        S ++      D EE   + + +
Sbjct: 359 RTLPPGLLPYLRLVALGGTDAFLLESLFRDTIWGHLELSVSR------DNEELLCKAVRE 418

Query: 432 SCETSISKYNKFLQ-----ASGSMD--LDVTSPKQLNRRVFLKQLAVDLCTSERRI-LFR 479
           +C+++++ Y+  ++       G++D  L +    +   ++ L+Q  +D    ++ + L +
Sbjct: 419 ACKSALAGYHTTIEQDRELKEGNLDSRLAIAVGIREGEKMVLQQ--IDGIFEQKELELDQ 472

BLAST of CSPI07G02590 vs. ExPASy Swiss-Prot
Match: B0VX69 (Actin-histidine N-methyltransferase OS=Callithrix jacchus OX=9483 GN=SETD3 PE=3 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 1.9e-23
Identity = 85/313 (27.16%), Postives = 150/313 (47.92%), Query Frame = 0

Query: 70  LQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAESEWSCPEAG 129
           L KW SE+G   +   +        GL A ++++  E  L+VP  L+++ ES  +    G
Sbjct: 82  LMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELFLWVPRKLLMTVESAKN-SVLG 141

Query: 130 EVLKRNSVPDWP---LIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREELDRYLE 189
            +  ++ +        +A +L+ E +   +S W  YI  LP +  + LY+  EE+ RYL+
Sbjct: 142 PLYSQDRILQAMGNIALAFHLLCERA-SPNSFWQPYIQTLPSEYDTPLYFEEEEV-RYLQ 201

Query: 190 ASEIRERAIERITNVVGTYNDLSIRVFSKHP---ELFPEEVFNIETFKWSFGILFSRLVR 249
           +++       +  N    Y     +V   HP   +L  ++ F  E ++W+   + +R  +
Sbjct: 202 STQAVHDVFSQYKNTARQY-AYFYKVIQTHPHANKLPLKDSFTYEDYRWAVSSVMTRQNQ 261

Query: 250 LPSMDGK---VALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 309
           +P+ DG    +AL+P  DM NH   + T   Y+           + ++ GEQ++I YG +
Sbjct: 262 IPTEDGSRVTLALIPLWDMCNHTNGLIT-TGYNLEDDRCECVALQDFRAGEQIYIFYGTR 321

Query: 310 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 369
           SN E ++  GF     +N  D V++ L + KSD+ Y  K E L + G+  S  F +  T 
Sbjct: 322 SNAEFVIHSGFF--FDNNSHDRVKIKLGVSKSDRLYAMKAEVLARAGIPTSSVFALHFTE 381

Query: 370 WPLELKAFAYLAV 374
            P+  +  A+L V
Sbjct: 382 PPISAQLLAFLRV 387

BLAST of CSPI07G02590 vs. ExPASy Swiss-Prot
Match: B5FW36 (Actin-histidine N-methyltransferase OS=Otolemur garnettii OX=30611 GN=SETD3 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 3.2e-23
Identity = 84/313 (26.84%), Postives = 151/313 (48.24%), Query Frame = 0

Query: 70  LQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAESEWSCPEAG 129
           L KW SE+G   +   +        GL A ++++  E  L+VP  L+++ ES  +    G
Sbjct: 82  LMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELFLWVPRKLLMTVESAKN-SVLG 141

Query: 130 EVLKRNSVPDWP---LIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREELDRYLE 189
            +  ++ +        +A +L+ E +   +S W  YI +LP +  + LY+  +E+ RYL+
Sbjct: 142 PLYSQDRILQAMGNIALAFHLLCERA-SPNSFWQPYIQSLPSEYDTPLYFEEDEV-RYLQ 201

Query: 190 ASEIRERAIERITNVVGTYNDLSIRVFSKHP---ELFPEEVFNIETFKWSFGILFSRLVR 249
           +++       +  N    Y     +V   HP   +L  ++ F  E ++W+   + +R  +
Sbjct: 202 STQAIHDVFSQYKNTARQY-AYFYKVIQTHPHANKLPLKDSFTYEDYRWAVSSVMTRQNQ 261

Query: 250 LPSMDGK---VALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 309
           +P+ DG    +AL+P  DM NH   + T   Y+           + ++ GEQ++I YG +
Sbjct: 262 IPTEDGSRVTLALIPLWDMCNHTNGLIT-TGYNLEDDRCECVALQDFRAGEQIYIFYGTR 321

Query: 310 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 369
           SN E ++  GF     +N  D V++ L + KSD+ Y  K E L + G+  S  F +  T 
Sbjct: 322 SNAEFVIHSGFF--FDNNSHDRVKIKLGVSKSDRLYAMKAEVLARAGIPTSSVFALHFTE 381

Query: 370 WPLELKAFAYLAV 374
            P+  +  A+L V
Sbjct: 382 PPISAQLLAFLRV 387

BLAST of CSPI07G02590 vs. ExPASy Swiss-Prot
Match: Q86TU7 (Actin-histidine N-methyltransferase OS=Homo sapiens OX=9606 GN=SETD3 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 4.1e-23
Identity = 84/313 (26.84%), Postives = 150/313 (47.92%), Query Frame = 0

Query: 70  LQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAESEWSCPEAG 129
           L KW SE+G   +   +        GL A ++++  E  L+VP  L+++ ES  +    G
Sbjct: 82  LMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELFLWVPRKLLMTVESAKN-SVLG 141

Query: 130 EVLKRNSVPDWP---LIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREELDRYLE 189
            +  ++ +        +A +L+ E +   +S W  YI  LP +  + LY+  +E+ RYL+
Sbjct: 142 PLYSQDRILQAMGNIALAFHLLCERA-SPNSFWQPYIQTLPSEYDTPLYFEEDEV-RYLQ 201

Query: 190 ASEIRERAIERITNVVGTYNDLSIRVFSKHP---ELFPEEVFNIETFKWSFGILFSRLVR 249
           +++       +  N    Y     +V   HP   +L  ++ F  E ++W+   + +R  +
Sbjct: 202 STQAIHDVFSQYKNTARQY-AYFYKVIQTHPHANKLPLKDSFTYEDYRWAVSSVMTRQNQ 261

Query: 250 LPSMDGK---VALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 309
           +P+ DG    +AL+P  DM NH   + T   Y+           + ++ GEQ++I YG +
Sbjct: 262 IPTEDGSRVTLALIPLWDMCNHTNGLIT-TGYNLEDDRCECVALQDFRAGEQIYIFYGTR 321

Query: 310 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 369
           SN E ++  GF     +N  D V++ L + KSD+ Y  K E L + G+  S  F +  T 
Sbjct: 322 SNAEFVIHSGFF--FDNNSHDRVKIKLGVSKSDRLYAMKAEVLARAGIPTSSVFALHFTE 381

Query: 370 WPLELKAFAYLAV 374
            P+  +  A+L V
Sbjct: 382 PPISAQLLAFLRV 387

BLAST of CSPI07G02590 vs. ExPASy Swiss-Prot
Match: B1MTJ4 (Actin-histidine N-methyltransferase OS=Plecturocebus moloch OX=9523 GN=SETD3 PE=3 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 4.1e-23
Identity = 84/313 (26.84%), Postives = 150/313 (47.92%), Query Frame = 0

Query: 70  LQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAESEWSCPEAG 129
           L KW SE+G   +   +        GL A ++++  E  L+VP  L+++ ES  +    G
Sbjct: 82  LMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELFLWVPRKLLMTVESAKN-SVLG 141

Query: 130 EVLKRNSVPDWP---LIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREELDRYLE 189
            +  ++ +        +A +L+ E +   +S W  YI  LP +  + LY+  +E+ RYL+
Sbjct: 142 PLYSQDRILQAMGNIALAFHLLCERA-SPNSFWQPYIQTLPSEYDTPLYFEEDEV-RYLQ 201

Query: 190 ASEIRERAIERITNVVGTYNDLSIRVFSKHP---ELFPEEVFNIETFKWSFGILFSRLVR 249
           +++       +  N    Y     +V   HP   +L  ++ F  E ++W+   + +R  +
Sbjct: 202 STQAIHDVFSQYKNTARQY-AYFYKVIQTHPHANKLPLKDSFTYEDYRWAVSSVMTRQNQ 261

Query: 250 LPSMDGK---VALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 309
           +P+ DG    +AL+P  DM NH   + T   Y+           + ++ GEQ++I YG +
Sbjct: 262 IPTEDGSRVTLALIPLWDMCNHTNGLIT-TGYNLEDDRCECVALQDFRAGEQIYIFYGTR 321

Query: 310 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 369
           SN E ++  GF     +N  D V++ L + KSD+ Y  K E L + G+  S  F +  T 
Sbjct: 322 SNAEFVIHSGFF--FDNNSHDRVKIKLGVSKSDRLYAMKAEVLARAGIPTSSVFALHFTE 381

Query: 370 WPLELKAFAYLAV 374
            P+  +  A+L V
Sbjct: 382 PPISAQLLAFLRV 387

BLAST of CSPI07G02590 vs. ExPASy TrEMBL
Match: A0A0A0K132 (SET domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G031610 PE=4 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 5.4e-284
Identity = 496/497 (99.80%), Postives = 496/497 (99.80%), Query Frame = 0

Query: 1   MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDSARVAATGPIPWGCE 60
           MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTD ARVAATGPIPWGCE
Sbjct: 1   MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDGARVAATGPIPWGCE 60

Query: 61  IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE 120
           IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE
Sbjct: 61  IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE 120

Query: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180
           SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE
Sbjct: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180

Query: 181 LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR 240
           LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR
Sbjct: 181 LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR 240

Query: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300
           LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK
Sbjct: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300

Query: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 360
           SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG
Sbjct: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 360

Query: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI 420
           WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI
Sbjct: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI 420

Query: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480
           SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS
Sbjct: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480

Query: 481 GELRALKLFRGFGKLFK 498
           GELRALKLFRGFGKLFK
Sbjct: 481 GELRALKLFRGFGKLFK 497

BLAST of CSPI07G02590 vs. ExPASy TrEMBL
Match: A0A1S3CFZ4 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500503 PE=4 SV=1)

HSP 1 Score: 963.4 bits (2489), Expect = 3.7e-277
Identity = 482/497 (96.98%), Postives = 491/497 (98.79%), Query Frame = 0

Query: 1   MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDSARVAATGPIPWGCE 60
           MAEASRVFNSSL+PNFRPLQ T STKPH ATFRRHSINCSVSTTD+ARVAATGPIPWGC+
Sbjct: 1   MAEASRVFNSSLVPNFRPLQYTHSTKPHAATFRRHSINCSVSTTDTARVAATGPIPWGCD 60

Query: 61  IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE 120
           IDSLENASALQKWLSESGLPDQKMSIQRV+VGERGLVALKNVRKGEKLLFVPPSLVISAE
Sbjct: 61  IDSLENASALQKWLSESGLPDQKMSIQRVDVGERGLVALKNVRKGEKLLFVPPSLVISAE 120

Query: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180
           SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRW+NYISALPRQPYSLLYWTREE
Sbjct: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWSNYISALPRQPYSLLYWTREE 180

Query: 181 LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR 240
           LDRYLEASEIRERAIERITNVVGTYNDL +RVFSKHPELFPEEVFNIETFKWSFGILFSR
Sbjct: 181 LDRYLEASEIRERAIERITNVVGTYNDLRVRVFSKHPELFPEEVFNIETFKWSFGILFSR 240

Query: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300
           LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK
Sbjct: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300

Query: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 360
           SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQ+TG
Sbjct: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQITG 360

Query: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI 420
           WPLELKAFAYLAVSPPSLSNQFDEMAAA SNKSTAKKDL+YPDIEEEA+QFILDSCETSI
Sbjct: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAATSNKSTAKKDLSYPDIEEEAMQFILDSCETSI 420

Query: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480
           SKYNKFLQASGSMDLDVT PKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS
Sbjct: 421 SKYNKFLQASGSMDLDVTLPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480

Query: 481 GELRALKLFRGFGKLFK 498
           GELRALKLFRGFGKLFK
Sbjct: 481 GELRALKLFRGFGKLFK 497

BLAST of CSPI07G02590 vs. ExPASy TrEMBL
Match: A0A6J1GKA5 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455093 PE=4 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 1.8e-271
Identity = 471/497 (94.77%), Postives = 486/497 (97.79%), Query Frame = 0

Query: 1   MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDSARVAATGPIPWGCE 60
           MAEASR+F S L+PNFRPLQ T S KP+TA+FRRHSINCSVST+DSARV ATGPIPWGCE
Sbjct: 1   MAEASRIFYSPLVPNFRPLQKTHSAKPYTASFRRHSINCSVSTSDSARVVATGPIPWGCE 60

Query: 61  IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE 120
           IDSLENASALQKWLS+SGLPDQKM+IQRV+VGERGLVALKNVRKGEKLLFVPPSLVISA+
Sbjct: 61  IDSLENASALQKWLSQSGLPDQKMAIQRVDVGERGLVALKNVRKGEKLLFVPPSLVISAD 120

Query: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180
           SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE
Sbjct: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180

Query: 181 LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR 240
           LDRYLEASEIRERAIERITNVVGTYNDL  R+FSK+PELFPEEVFNIETFKWSFGILFSR
Sbjct: 181 LDRYLEASEIRERAIERITNVVGTYNDLRDRIFSKYPELFPEEVFNIETFKWSFGILFSR 240

Query: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300
           LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK
Sbjct: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300

Query: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 360
           SNGELLLSYGFVPKEG+NPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQ+TG
Sbjct: 301 SNGELLLSYGFVPKEGTNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQITG 360

Query: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI 420
           WPLELKAFAYLAVSPPS+S QF+EMAAAASNKST  KDLNYPDIEEEALQFILDSCETSI
Sbjct: 361 WPLELKAFAYLAVSPPSMSKQFEEMAAAASNKSTTTKDLNYPDIEEEALQFILDSCETSI 420

Query: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480
           SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS
Sbjct: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480

Query: 481 GELRALKLFRGFGKLFK 498
           GELRALKLFRGFGKLFK
Sbjct: 481 GELRALKLFRGFGKLFK 497

BLAST of CSPI07G02590 vs. ExPASy TrEMBL
Match: A0A6J1HWK3 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468148 PE=4 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 4.0e-271
Identity = 469/497 (94.37%), Postives = 488/497 (98.19%), Query Frame = 0

Query: 1   MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDSARVAATGPIPWGCE 60
           MAEASR+F S L+PNFRPLQ T STKP+TA+FRRHSINCSVST+DSARVAATGPIPWGCE
Sbjct: 126 MAEASRIFYSPLVPNFRPLQKTHSTKPYTASFRRHSINCSVSTSDSARVAATGPIPWGCE 185

Query: 61  IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE 120
           IDSLEN+SALQKWLS+SGLPDQKM+IQRV+VGERGLVALKNVRKGEKLLFVPPSLVISA+
Sbjct: 186 IDSLENSSALQKWLSQSGLPDQKMAIQRVDVGERGLVALKNVRKGEKLLFVPPSLVISAD 245

Query: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180
           SEWSCPEAG+VLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE
Sbjct: 246 SEWSCPEAGDVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 305

Query: 181 LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR 240
           LDRYLEASEIRERAIERITNVVGTYNDL  R+FSK+PELFPEEVFNIETFKWSFGILFSR
Sbjct: 306 LDRYLEASEIRERAIERITNVVGTYNDLRDRIFSKYPELFPEEVFNIETFKWSFGILFSR 365

Query: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300
           LVRLP+MDGKVALVPWADMLNHNCEVETFLDYDKASQG+VFTTDRAYQPGEQVFISYGKK
Sbjct: 366 LVRLPAMDGKVALVPWADMLNHNCEVETFLDYDKASQGIVFTTDRAYQPGEQVFISYGKK 425

Query: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 360
           SNGELLLSYGFVPKEG+NPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQ+TG
Sbjct: 426 SNGELLLSYGFVPKEGTNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQITG 485

Query: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI 420
           WPLELKAFAYLAVSPPS+S QF+EMAAAASNKSTA KDLNYPDIEEEALQFILDSCE SI
Sbjct: 486 WPLELKAFAYLAVSPPSMSKQFEEMAAAASNKSTATKDLNYPDIEEEALQFILDSCEMSI 545

Query: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480
           SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS
Sbjct: 546 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 605

Query: 481 GELRALKLFRGFGKLFK 498
           GELRALKLFRGFGKLFK
Sbjct: 606 GELRALKLFRGFGKLFK 622

BLAST of CSPI07G02590 vs. ExPASy TrEMBL
Match: A0A6J1HV83 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111468148 PE=4 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 4.0e-271
Identity = 469/497 (94.37%), Postives = 488/497 (98.19%), Query Frame = 0

Query: 1   MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDSARVAATGPIPWGCE 60
           MAEASR+F S L+PNFRPLQ T STKP+TA+FRRHSINCSVST+DSARVAATGPIPWGCE
Sbjct: 111 MAEASRIFYSPLVPNFRPLQKTHSTKPYTASFRRHSINCSVSTSDSARVAATGPIPWGCE 170

Query: 61  IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE 120
           IDSLEN+SALQKWLS+SGLPDQKM+IQRV+VGERGLVALKNVRKGEKLLFVPPSLVISA+
Sbjct: 171 IDSLENSSALQKWLSQSGLPDQKMAIQRVDVGERGLVALKNVRKGEKLLFVPPSLVISAD 230

Query: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180
           SEWSCPEAG+VLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE
Sbjct: 231 SEWSCPEAGDVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 290

Query: 181 LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR 240
           LDRYLEASEIRERAIERITNVVGTYNDL  R+FSK+PELFPEEVFNIETFKWSFGILFSR
Sbjct: 291 LDRYLEASEIRERAIERITNVVGTYNDLRDRIFSKYPELFPEEVFNIETFKWSFGILFSR 350

Query: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300
           LVRLP+MDGKVALVPWADMLNHNCEVETFLDYDKASQG+VFTTDRAYQPGEQVFISYGKK
Sbjct: 351 LVRLPAMDGKVALVPWADMLNHNCEVETFLDYDKASQGIVFTTDRAYQPGEQVFISYGKK 410

Query: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 360
           SNGELLLSYGFVPKEG+NPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQ+TG
Sbjct: 411 SNGELLLSYGFVPKEGTNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQITG 470

Query: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI 420
           WPLELKAFAYLAVSPPS+S QF+EMAAAASNKSTA KDLNYPDIEEEALQFILDSCE SI
Sbjct: 471 WPLELKAFAYLAVSPPSMSKQFEEMAAAASNKSTATKDLNYPDIEEEALQFILDSCEMSI 530

Query: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480
           SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS
Sbjct: 531 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 590

Query: 481 GELRALKLFRGFGKLFK 498
           GELRALKLFRGFGKLFK
Sbjct: 591 GELRALKLFRGFGKLFK 607

BLAST of CSPI07G02590 vs. NCBI nr
Match: XP_004144553.1 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic [Cucumis sativus] >KGN43400.1 hypothetical protein Csa_020168 [Cucumis sativus])

HSP 1 Score: 986.1 bits (2548), Expect = 1.1e-283
Identity = 496/497 (99.80%), Postives = 496/497 (99.80%), Query Frame = 0

Query: 1   MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDSARVAATGPIPWGCE 60
           MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTD ARVAATGPIPWGCE
Sbjct: 1   MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDGARVAATGPIPWGCE 60

Query: 61  IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE 120
           IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE
Sbjct: 61  IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE 120

Query: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180
           SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE
Sbjct: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180

Query: 181 LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR 240
           LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR
Sbjct: 181 LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR 240

Query: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300
           LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK
Sbjct: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300

Query: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 360
           SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG
Sbjct: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 360

Query: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI 420
           WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI
Sbjct: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI 420

Query: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480
           SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS
Sbjct: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480

Query: 481 GELRALKLFRGFGKLFK 498
           GELRALKLFRGFGKLFK
Sbjct: 481 GELRALKLFRGFGKLFK 497

BLAST of CSPI07G02590 vs. NCBI nr
Match: XP_008462040.1 (PREDICTED: ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic [Cucumis melo])

HSP 1 Score: 963.4 bits (2489), Expect = 7.7e-277
Identity = 482/497 (96.98%), Postives = 491/497 (98.79%), Query Frame = 0

Query: 1   MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDSARVAATGPIPWGCE 60
           MAEASRVFNSSL+PNFRPLQ T STKPH ATFRRHSINCSVSTTD+ARVAATGPIPWGC+
Sbjct: 1   MAEASRVFNSSLVPNFRPLQYTHSTKPHAATFRRHSINCSVSTTDTARVAATGPIPWGCD 60

Query: 61  IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE 120
           IDSLENASALQKWLSESGLPDQKMSIQRV+VGERGLVALKNVRKGEKLLFVPPSLVISAE
Sbjct: 61  IDSLENASALQKWLSESGLPDQKMSIQRVDVGERGLVALKNVRKGEKLLFVPPSLVISAE 120

Query: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180
           SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRW+NYISALPRQPYSLLYWTREE
Sbjct: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWSNYISALPRQPYSLLYWTREE 180

Query: 181 LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR 240
           LDRYLEASEIRERAIERITNVVGTYNDL +RVFSKHPELFPEEVFNIETFKWSFGILFSR
Sbjct: 181 LDRYLEASEIRERAIERITNVVGTYNDLRVRVFSKHPELFPEEVFNIETFKWSFGILFSR 240

Query: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300
           LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK
Sbjct: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300

Query: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 360
           SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQ+TG
Sbjct: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQITG 360

Query: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI 420
           WPLELKAFAYLAVSPPSLSNQFDEMAAA SNKSTAKKDL+YPDIEEEA+QFILDSCETSI
Sbjct: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAATSNKSTAKKDLSYPDIEEEAMQFILDSCETSI 420

Query: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480
           SKYNKFLQASGSMDLDVT PKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS
Sbjct: 421 SKYNKFLQASGSMDLDVTLPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480

Query: 481 GELRALKLFRGFGKLFK 498
           GELRALKLFRGFGKLFK
Sbjct: 481 GELRALKLFRGFGKLFK 497

BLAST of CSPI07G02590 vs. NCBI nr
Match: XP_023554432.1 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023554433.1 ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 948.3 bits (2450), Expect = 2.6e-272
Identity = 473/497 (95.17%), Postives = 489/497 (98.39%), Query Frame = 0

Query: 1   MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDSARVAATGPIPWGCE 60
           MAEASR+F S L+PNFRPLQ T STKP+TA+FRRHSINCSVST+DSARVAATGPIPWGCE
Sbjct: 126 MAEASRIFYSPLVPNFRPLQKTHSTKPYTASFRRHSINCSVSTSDSARVAATGPIPWGCE 185

Query: 61  IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE 120
           IDSLENASALQKWLS+SGLPDQKM+IQRV+VGERGLVALKNVRKGEKLLFVPPSLVISA+
Sbjct: 186 IDSLENASALQKWLSQSGLPDQKMAIQRVDVGERGLVALKNVRKGEKLLFVPPSLVISAD 245

Query: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180
           SEWSCPEAGE+LKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE
Sbjct: 246 SEWSCPEAGEMLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 305

Query: 181 LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR 240
           LDRYLEASEIRERAIERITNVVGTYNDL  R+FSK+PELFPEEVFNIETFKWSFGILFSR
Sbjct: 306 LDRYLEASEIRERAIERITNVVGTYNDLRDRIFSKYPELFPEEVFNIETFKWSFGILFSR 365

Query: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300
           LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDK+SQGVVFTTDRAYQPGEQVFISYGKK
Sbjct: 366 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKSSQGVVFTTDRAYQPGEQVFISYGKK 425

Query: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 360
           SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQ+TG
Sbjct: 426 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQITG 485

Query: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI 420
           WPLELKAFAYLAVSPPS+S QF+EMAAAASNKSTA KDLNYPDIEEEALQFILDSCETSI
Sbjct: 486 WPLELKAFAYLAVSPPSMSKQFEEMAAAASNKSTATKDLNYPDIEEEALQFILDSCETSI 545

Query: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480
           SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS
Sbjct: 546 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 605

Query: 481 GELRALKLFRGFGKLFK 498
           GELRALKLFRGFGKLFK
Sbjct: 606 GELRALKLFRGFGKLFK 622

BLAST of CSPI07G02590 vs. NCBI nr
Match: XP_022952392.1 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic [Cucurbita moschata] >XP_022952393.1 ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 944.5 bits (2440), Expect = 3.7e-271
Identity = 471/497 (94.77%), Postives = 486/497 (97.79%), Query Frame = 0

Query: 1   MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDSARVAATGPIPWGCE 60
           MAEASR+F S L+PNFRPLQ T S KP+TA+FRRHSINCSVST+DSARV ATGPIPWGCE
Sbjct: 1   MAEASRIFYSPLVPNFRPLQKTHSAKPYTASFRRHSINCSVSTSDSARVVATGPIPWGCE 60

Query: 61  IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE 120
           IDSLENASALQKWLS+SGLPDQKM+IQRV+VGERGLVALKNVRKGEKLLFVPPSLVISA+
Sbjct: 61  IDSLENASALQKWLSQSGLPDQKMAIQRVDVGERGLVALKNVRKGEKLLFVPPSLVISAD 120

Query: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180
           SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE
Sbjct: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180

Query: 181 LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR 240
           LDRYLEASEIRERAIERITNVVGTYNDL  R+FSK+PELFPEEVFNIETFKWSFGILFSR
Sbjct: 181 LDRYLEASEIRERAIERITNVVGTYNDLRDRIFSKYPELFPEEVFNIETFKWSFGILFSR 240

Query: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300
           LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK
Sbjct: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300

Query: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 360
           SNGELLLSYGFVPKEG+NPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQ+TG
Sbjct: 301 SNGELLLSYGFVPKEGTNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQITG 360

Query: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI 420
           WPLELKAFAYLAVSPPS+S QF+EMAAAASNKST  KDLNYPDIEEEALQFILDSCETSI
Sbjct: 361 WPLELKAFAYLAVSPPSMSKQFEEMAAAASNKSTTTKDLNYPDIEEEALQFILDSCETSI 420

Query: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480
           SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS
Sbjct: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480

Query: 481 GELRALKLFRGFGKLFK 498
           GELRALKLFRGFGKLFK
Sbjct: 481 GELRALKLFRGFGKLFK 497

BLAST of CSPI07G02590 vs. NCBI nr
Match: XP_022969030.1 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic isoform X1 [Cucurbita maxima] >XP_022969031.1 ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 943.3 bits (2437), Expect = 8.2e-271
Identity = 469/497 (94.37%), Postives = 488/497 (98.19%), Query Frame = 0

Query: 1   MAEASRVFNSSLLPNFRPLQNTLSTKPHTATFRRHSINCSVSTTDSARVAATGPIPWGCE 60
           MAEASR+F S L+PNFRPLQ T STKP+TA+FRRHSINCSVST+DSARVAATGPIPWGCE
Sbjct: 126 MAEASRIFYSPLVPNFRPLQKTHSTKPYTASFRRHSINCSVSTSDSARVAATGPIPWGCE 185

Query: 61  IDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAE 120
           IDSLEN+SALQKWLS+SGLPDQKM+IQRV+VGERGLVALKNVRKGEKLLFVPPSLVISA+
Sbjct: 186 IDSLENSSALQKWLSQSGLPDQKMAIQRVDVGERGLVALKNVRKGEKLLFVPPSLVISAD 245

Query: 121 SEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 180
           SEWSCPEAG+VLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE
Sbjct: 246 SEWSCPEAGDVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTREE 305

Query: 181 LDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSR 240
           LDRYLEASEIRERAIERITNVVGTYNDL  R+FSK+PELFPEEVFNIETFKWSFGILFSR
Sbjct: 306 LDRYLEASEIRERAIERITNVVGTYNDLRDRIFSKYPELFPEEVFNIETFKWSFGILFSR 365

Query: 241 LVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKK 300
           LVRLP+MDGKVALVPWADMLNHNCEVETFLDYDKASQG+VFTTDRAYQPGEQVFISYGKK
Sbjct: 366 LVRLPAMDGKVALVPWADMLNHNCEVETFLDYDKASQGIVFTTDRAYQPGEQVFISYGKK 425

Query: 301 SNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQVTG 360
           SNGELLLSYGFVPKEG+NPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQ+TG
Sbjct: 426 SNGELLLSYGFVPKEGTNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQCFPIQITG 485

Query: 361 WPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSI 420
           WPLELKAFAYLAVSPPS+S QF+EMAAAASNKSTA KDLNYPDIEEEALQFILDSCE SI
Sbjct: 486 WPLELKAFAYLAVSPPSMSKQFEEMAAAASNKSTATKDLNYPDIEEEALQFILDSCEMSI 545

Query: 421 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 480
           SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS
Sbjct: 546 SKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRLRDLRS 605

Query: 481 GELRALKLFRGFGKLFK 498
           GELRALKLFRGFGKLFK
Sbjct: 606 GELRALKLFRGFGKLFK 622

BLAST of CSPI07G02590 vs. TAIR 10
Match: AT3G07670.1 (Rubisco methyltransferase family protein )

HSP 1 Score: 750.7 bits (1937), Expect = 7.3e-217
Identity = 378/505 (74.85%), Postives = 431/505 (85.35%), Query Frame = 0

Query: 1   MAEASRVFNSSLLPNFRPL-----QN-TLSTKPHTATFRRHSINCSVST--TDSARVAAT 60
           MA+A  +  S+LLP + PL     QN TLS  P   +  R  I+CSVS   T    +   
Sbjct: 1   MAKAC-LLQSTLLPAYSPLHKLRNQNITLSFSPLPLSRCRPGIHCSVSAGETTIQSMEEA 60

Query: 61  GPIPWGCEIDSLENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVP 120
             I WGCEIDSLENA++LQ WLS+SGLP QKM+I RV++GERGLVA +N+RKGEKLLFVP
Sbjct: 61  PKISWGCEIDSLENATSLQNWLSDSGLPPQKMAIDRVDIGERGLVASQNLRKGEKLLFVP 120

Query: 121 PSLVISAESEWSCPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYS 180
           PSLVISA+SEW+  EAGEV+KR  VPDWPL+ATYLISEASL KSSRW NYISALPRQPYS
Sbjct: 121 PSLVISADSEWTNAEAGEVMKRYDVPDWPLLATYLISEASLQKSSRWFNYISALPRQPYS 180

Query: 181 LLYWTREELDRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKW 240
           LLYWTR ELD YLEAS+IRERAIERITNVVGTY DL  R+FSKHP+LFP+EVFN ETFKW
Sbjct: 181 LLYWTRTELDMYLEASQIRERAIERITNVVGTYEDLRSRIFSKHPQLFPKEVFNDETFKW 240

Query: 241 SFGILFSRLVRLPSMDGKVALVPWADMLNHNCEVETFLDYDKASQGVVFTTDRAYQPGEQ 300
           SFGILFSRLVRLPSMDG+ ALVPWADMLNHNCEVETFLDYDK+S+GVVFTTDR YQPGEQ
Sbjct: 241 SFGILFSRLVRLPSMDGRFALVPWADMLNHNCEVETFLDYDKSSKGVVFTTDRPYQPGEQ 300

Query: 301 VFISYGKKSNGELLLSYGFVPKEGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRASQ 360
           VFISYG KSNGELLLSYGFVP+EG+NPSDSVEL LSL+K+DKCY+EKL+ALKKHGL   Q
Sbjct: 301 VFISYGNKSNGELLLSYGFVPREGTNPSDSVELALSLRKNDKCYEEKLDALKKHGLSTPQ 360

Query: 361 CFPIQVTGWPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFI 420
           CFP+++TGWP+EL A+AYL VSPP + N F+EMA AASNK++ K DL YP+IEE+ALQFI
Sbjct: 361 CFPVRITGWPMELMAYAYLVVSPPDMRNNFEEMAKAASNKTSTKNDLKYPEIEEDALQFI 420

Query: 421 LDSCETSISKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILR 480
           LDSCETSISKY++FL+ SGSMDLD+TSPKQLNR+ FLKQLAVDL TSERRIL+R+QYILR
Sbjct: 421 LDSCETSISKYSRFLKESGSMDLDITSPKQLNRKAFLKQLAVDLSTSERRILYRAQYILR 480

Query: 481 RRLRDLRSGELRALKLFRGFGKLFK 498
           RRLRD+RSGEL+AL+LF G    FK
Sbjct: 481 RRLRDIRSGELKALRLFSGLRNFFK 504

BLAST of CSPI07G02590 vs. TAIR 10
Match: AT3G55080.1 (SET domain-containing protein )

HSP 1 Score: 104.0 bits (258), Expect = 3.6e-22
Identity = 104/425 (24.47%), Postives = 193/425 (45.41%), Query Frame = 0

Query: 84  MSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAESEWSCPEAGEVLKRNSVPDWPLI 143
           +SI +   G R L A K +  G+ +L VP +  I+ +     P    VL  N V +  ++
Sbjct: 62  LSIGKSTYG-RSLFASKVIYAGDCMLKVPFNAQITPD---ELPSDIRVLLSNEVGNIGML 121

Query: 144 ATYLISEASLMKSSRWNNYISALPR--QPYSLLYWTREELDRYLEASEIRERAIERITNV 203
           A  LI E  + + SRW  YIS LP+  + +S ++W  +EL   +  S + +  +++   +
Sbjct: 122 AAVLIREKKMGQKSRWVPYISRLPQPAEMHSSIFWGEDELS-MIRCSAVHQETVKQKAQI 181

Query: 204 VGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSRLVRLPSMDGKVALVPWADMLN 263
              ++ ++ + F +H  +  E   ++E F +++ ++ SR         +++L+P+AD +N
Sbjct: 182 EKDFSFVA-QAFKQHCPIVTERP-DLEDFMYAYALVGSRAW---ENSKRISLIPFADFMN 241

Query: 264 HNCEVETFLDYDKASQGVVFTTDRAYQPGEQVFISYGKKSNGELLLSYGFVPKEGSNPSD 323
           H+    + +  D+ +Q    T DR Y PG++VFI YG+ SN  L+L +GF      N  D
Sbjct: 242 HDGLSASIVLRDEDNQLSEVTADRNYSPGDEVFIKYGEFSNATLMLDFGFT--FPYNIHD 301

Query: 324 SVELLLSLKKSDKCYKEKLEALKKHGLRA--------SQCFPIQV----------TGWPL 383
            V++ + +   D     KL  L+ H  R         S C    +           G P 
Sbjct: 302 EVQIQMDVPNDDPLRNMKLGLLQTHHTRTVKDINIFHSSCDTFTIKEVKSAIGKGKGIPQ 361

Query: 384 ELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSCETSISKY 443
            L+AFA +      +  + ++++  A+        L + D   E     L++ +  +S  
Sbjct: 362 SLRAFARVLCC--IIPQELNDLSKEAAQNDGRLARLPFKDGNRE-----LEAHKILLSHI 421

Query: 444 NKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRL-RDLRSGE 488
           N+ ++               +  V +K++      S+R       + +RR++ RDL  GE
Sbjct: 422 NRLIE---------------DHSVCIKEMEECYFVSQR-------FAVRRQMARDLLYGE 445

BLAST of CSPI07G02590 vs. TAIR 10
Match: AT2G18850.1 (SET domain-containing protein )

HSP 1 Score: 91.3 bits (225), Expect = 2.4e-18
Identity = 74/268 (27.61%), Postives = 132/268 (49.25%), Query Frame = 0

Query: 65  ENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAESEWS 124
           E  S L +W  ++G+   K+ I +++   RG +A ++++ G+  L +P S +IS E  ++
Sbjct: 148 EKESKLVEWGQDNGV-KTKLQIAQIDGYGRGAIASEDLKFGDVALEIPVSSIISEEYVYN 207

Query: 125 CPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTRE---EL 184
                 +   + +    ++  + + E   +  S++  Y  +L     + L +  +   EL
Sbjct: 208 SDMYPILETFDGITSETMLLLWTMREKHNL-DSKFKPYFDSLQENFCTGLSFGVDAIMEL 267

Query: 185 DRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSRL 244
           D  L   EI + A E +      Y++L I + S H E+FP E++  E + W+  + +S  
Sbjct: 268 DGTLLLDEIMQ-AKELLRE---RYDEL-IPLLSNHREVFPPELYTWEHYLWACELYYSNS 327

Query: 245 VRLPSMDGKV--ALVPWADMLNHNC--EVETFLDYDKASQGVVFTTDRAYQPGEQVFISY 304
           +++   DGK+   L+P A  LNH+    +  +   D  +  + F   R    GEQ F+SY
Sbjct: 328 MQIKFPDGKLKTCLIPVAGFLNHSIYPHIVKYGKVDIETSSLKFPVSRPCNKGEQCFLSY 387

Query: 305 GKKSNGELLLSYGFVPKEGSNPSDSVEL 326
           G  S+  LL  YGF+PK G NP D + L
Sbjct: 388 GNYSSSHLLTFYGFLPK-GDNPYDVIPL 407

BLAST of CSPI07G02590 vs. TAIR 10
Match: AT2G18850.2 (SET domain-containing protein )

HSP 1 Score: 91.3 bits (225), Expect = 2.4e-18
Identity = 74/268 (27.61%), Postives = 132/268 (49.25%), Query Frame = 0

Query: 65  ENASALQKWLSESGLPDQKMSIQRVNVGERGLVALKNVRKGEKLLFVPPSLVISAESEWS 124
           E  S L +W  ++G+   K+ I +++   RG +A ++++ G+  L +P S +IS E  ++
Sbjct: 148 EKESKLVEWGQDNGV-KTKLQIAQIDGYGRGAIASEDLKFGDVALEIPVSSIISEEYVYN 207

Query: 125 CPEAGEVLKRNSVPDWPLIATYLISEASLMKSSRWNNYISALPRQPYSLLYWTRE---EL 184
                 +   + +    ++  + + E   +  S++  Y  +L     + L +  +   EL
Sbjct: 208 SDMYPILETFDGITSETMLLLWTMREKHNL-DSKFKPYFDSLQENFCTGLSFGVDAIMEL 267

Query: 185 DRYLEASEIRERAIERITNVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSRL 244
           D  L   EI + A E +      Y++L I + S H E+FP E++  E + W+  + +S  
Sbjct: 268 DGTLLLDEIMQ-AKELLRE---RYDEL-IPLLSNHREVFPPELYTWEHYLWACELYYSNS 327

Query: 245 VRLPSMDGKV--ALVPWADMLNHNC--EVETFLDYDKASQGVVFTTDRAYQPGEQVFISY 304
           +++   DGK+   L+P A  LNH+    +  +   D  +  + F   R    GEQ F+SY
Sbjct: 328 MQIKFPDGKLKTCLIPVAGFLNHSIYPHIVKYGKVDIETSSLKFPVSRPCNKGEQCFLSY 387

Query: 305 GKKSNGELLLSYGFVPKEGSNPSDSVEL 326
           G  S+  LL  YGF+PK G NP D + L
Sbjct: 388 GNYSSSHLLTFYGFLPK-GDNPYDVIPL 407

BLAST of CSPI07G02590 vs. TAIR 10
Match: AT3G55080.2 (SET domain-containing protein )

HSP 1 Score: 89.7 bits (221), Expect = 7.0e-18
Identity = 88/372 (23.66%), Postives = 169/372 (45.43%), Query Frame = 0

Query: 142 LIATYLISEASLMKSSRWNNYISALPR--QPYSLLYWTREELDRYLEASEIRERAIERIT 201
           ++A  LI E  + + SRW  YIS LP+  + +S ++W  +EL   +  S + +  +++  
Sbjct: 1   MLAAVLIREKKMGQKSRWVPYISRLPQPAEMHSSIFWGEDELS-MIRCSAVHQETVKQKA 60

Query: 202 NVVGTYNDLSIRVFSKHPELFPEEVFNIETFKWSFGILFSRLVRLPSMDGKVALVPWADM 261
            +   ++ ++ + F +H  +  E   ++E F +++ ++ SR         +++L+P+AD 
Sbjct: 61  QIEKDFSFVA-QAFKQHCPIVTERP-DLEDFMYAYALVGSRAW---ENSKRISLIPFADF 120

Query: 262 LNHNCEVETFLDYDKASQGVVFTT-----DRAYQPGEQVFISYGKKSNGELLLSYGFVPK 321
           +NH+    + +  D+ +Q   F+T     DR Y PG++VFI YG+ SN  L+L +GF   
Sbjct: 121 MNHDGLSASIVLRDEDNQLSEFSTLQVTADRNYSPGDEVFIKYGEFSNATLMLDFGFT-- 180

Query: 322 EGSNPSDSVELLLSLKKSDKCYKEKLEALKKHGLRA--------SQCFPIQV-------- 381
              N  D V++ + +   D     KL  L+ H  R         S C    +        
Sbjct: 181 FPYNIHDEVQIQMDVPNDDPLRNMKLGLLQTHHTRTVKDINIFHSSCDTFTIKEVKSAIG 240

Query: 382 --TGWPLELKAFAYLAVSPPSLSNQFDEMAAAASNKSTAKKDLNYPDIEEEALQFILDSC 441
              G P  L+AFA +      +  + ++++  A+        L + D   E     L++ 
Sbjct: 241 KGKGIPQSLRAFARVLCC--IIPQELNDLSKEAAQNDGRLARLPFKDGNRE-----LEAH 300

Query: 442 ETSISKYNKFLQASGSMDLDVTSPKQLNRRVFLKQLAVDLCTSERRILFRSQYILRRRL- 488
           +  +S  N+ ++               +  V +K++      S+R       + +RR++ 
Sbjct: 301 KILLSHINRLIE---------------DHSVCIKEMEECYFVSQR-------FAVRRQMA 335

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q430882.9e-2426.85Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
B0VX691.9e-2327.16Actin-histidine N-methyltransferase OS=Callithrix jacchus OX=9483 GN=SETD3 PE=3 ... [more]
B5FW363.2e-2326.84Actin-histidine N-methyltransferase OS=Otolemur garnettii OX=30611 GN=SETD3 PE=3... [more]
Q86TU74.1e-2326.84Actin-histidine N-methyltransferase OS=Homo sapiens OX=9606 GN=SETD3 PE=1 SV=1[more]
B1MTJ44.1e-2326.84Actin-histidine N-methyltransferase OS=Plecturocebus moloch OX=9523 GN=SETD3 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0K1325.4e-28499.80SET domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G031610 PE=4 SV... [more]
A0A1S3CFZ43.7e-27796.98ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
A0A6J1GKA51.8e-27194.77ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
A0A6J1HWK34.0e-27194.37ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
A0A6J1HV834.0e-27194.37ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
Match NameE-valueIdentityDescription
XP_004144553.11.1e-28399.80ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
XP_008462040.17.7e-27796.98PREDICTED: ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methy... [more]
XP_023554432.12.6e-27295.17ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
XP_022952392.13.7e-27194.77ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
XP_022969030.18.2e-27194.37ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
Match NameE-valueIdentityDescription
AT3G07670.17.3e-21774.85Rubisco methyltransferase family protein [more]
AT3G55080.13.6e-2224.47SET domain-containing protein [more]
AT2G18850.12.4e-1827.61SET domain-containing protein [more]
AT2G18850.22.4e-1827.61SET domain-containing protein [more]
AT3G55080.27.0e-1823.66SET domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001214SET domainSMARTSM00317set_7coord: 82..304
e-value: 2.2E-10
score: 50.5
IPR001214SET domainPFAMPF00856SETcoord: 94..298
e-value: 9.8E-15
score: 55.4
IPR001214SET domainPROSITEPS50280SETcoord: 82..298
score: 11.516924
IPR015353Rubisco LSMT, substrate-binding domainPFAMPF09273Rubis-subs-bindcoord: 332..464
e-value: 1.3E-19
score: 71.1
IPR011192Rubisco LSMT methyltransferase, plantPIRSFPIRSF009328RMT_SETcoord: 1..493
e-value: 1.1E-162
score: 539.7
NoneNo IPR availableGENE3D3.90.1410.10set domain protein methyltransferase, domain 1coord: 48..311
e-value: 4.7E-59
score: 202.1
NoneNo IPR availablePIRSRPIRSR009328-1PIRSR009328-1coord: 67..489
e-value: 1.6E-161
score: 535.2
NoneNo IPR availablePANTHERPTHR13271UNCHARACTERIZED PUTATIVE METHYLTRANSFERASEcoord: 9..292
NoneNo IPR availablePANTHERPTHR13271:SF110SUBFAMILY NOT NAMEDcoord: 9..292
NoneNo IPR availableSUPERFAMILY82199SET domaincoord: 66..316
IPR036464Rubisco LSMT, substrate-binding domain superfamilyGENE3D3.90.1420.10coord: 316..479
e-value: 5.1E-28
score: 99.9
IPR036464Rubisco LSMT, substrate-binding domain superfamilySUPERFAMILY81822RuBisCo LSMT C-terminal, substrate-binding domaincoord: 328..495
IPR044431RBCMT, SET domainCDDcd19179SET_RBCMTcoord: 82..312
e-value: 2.30356E-89
score: 271.495

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G02590.1CSPI07G02590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018026 peptidyl-lysine monomethylation
biological_process GO:0018022 peptidyl-lysine methylation
cellular_component GO:0009507 chloroplast
molecular_function GO:0005515 protein binding
molecular_function GO:0016279 protein-lysine N-methyltransferase activity
molecular_function GO:0030785 [ribulose-bisphosphate carboxylase]-lysine N-methyltransferase activity