CSPI07G02130 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G02130
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMBOAT (membrane bound O-acyl transferase) family protein
LocationChr7: 1850305 .. 1851533 (-)
RNA-Seq ExpressionCSPI07G02130
SyntenyCSPI07G02130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCGGTTGATCGTGAAATGTGGCCAAACAATATGTTTATTGTAATAGTGTGTGTAAAGTTGTTTTACACTGTGCAAGTAACGTATATAATTCACGTTAAGTTTGAAGTTCAATAAAGATTAGACGACCATAGGCTCCATGGCAAGCACATCAGCTTGTTGTTAAGTATGAAAAAGGTATGTTAAAAGATTTCTTTTAACACCGAAAGAAGAGCCCAGAAATAAGCAAACAAAACAGAAAAGACTCCAAATTAAAAGAAACCAAACGCAAATCATCTAATATGGTTGGCGAAATCGTGAGATTCATCAAGGTGTTGCCAGTATTCATTTTGTCCTCTTTGTATTGCTATTTCATAGCTTCAAAGTTTTCCAAAGCCAAACTCCGGCTTCTCTCTCTCCTCCCTGTCTTCTCCATCTTCGTCGTCCTCCCTCTCTACTTCTCCTCCATCCTCTTCACTAGCAGTGTTGGCTTATTCGTCACCTGGCTTACCACCTTTAAGCTACTTCTTTTCACATTTGATTCGGGTCCTCTAGCTTCCAACCCACCATTGTCCTTCCCTCTTTTTGCTTCCATTGCTTTCTTTCCAACTAGAACCAAGCACAACAAAACAACCCCACATGATCAAAAATCGCTTCCAAAGTTATTGCCTCTAAACTTGCCAACCAAAGTGGTGATACTTGCCATATTGATCTTAGGAAACAGCTATATTGACCTTGTTTGTCCCAATAATGTAAAGTCATGGATCAATGGTGCTACCGTGTATTTCTACTTAGACGTAGTTATGAGTTTGTCCAATGTCTTTGTTCAATCAACTTTCGGCGTCGAGGTCAGACAACCATTCAACGAGCCATATCTGGCGACATCGCTTCAAAATTTATGGGGCCGGAGGTGGAATCTATTAGTATCTGAAACTTTGCACAGCACCATCTACAAACCAATTCGTTACAAAGTAGGGATGCCACGGTGGGTGGCAGTGGTGACTGTGTTTGTGGTGTCGGGGCTTATGCATGAGCTTTTGTATTACTATATGGTCAGAGCGAATCCGACGTGGGAATTCACGTGGTTCTTTGTGATTCATGGAGTGTGTTTGGCCTTGGAGATTGAATTGAAGAGGGCTTATGAGAAAAAATGGCAGCTACAAAGGGCAGTTTCTACACTATTAACCACGATGTTTACGGGAACCACCACTCTTTGGTTGCTTTTTCCTCTAATGTTGAAGACTTTAGCCTGA

mRNA sequence

TCGGTTGATCGTGAAATGTGGCCAAACAATATGTTTATTGTAATAGTGTGTGTAAAGTTGTTTTACACTGTGCAAGTAACGTATATAATTCACGTTAAGTTTGAAGTTCAATAAAGATTAGACGACCATAGGCTCCATGGCAAGCACATCAGCTTGTTGTTAAGTATGAAAAAGGTATGTTAAAAGATTTCTTTTAACACCGAAAGAAGAGCCCAGAAATAAGCAAACAAAACAGAAAAGACTCCAAATTAAAAGAAACCAAACGCAAATCATCTAATATGGTTGGCGAAATCGTGAGATTCATCAAGGTGTTGCCAGTATTCATTTTGTCCTCTTTGTATTGCTATTTCATAGCTTCAAAGTTTTCCAAAGCCAAACTCCGGCTTCTCTCTCTCCTCCCTGTCTTCTCCATCTTCGTCGTCCTCCCTCTCTACTTCTCCTCCATCCTCTTCACTAGCAGTGTTGGCTTATTCGTCACCTGGCTTACCACCTTTAAGCTACTTCTTTTCACATTTGATTCGGGTCCTCTAGCTTCCAACCCACCATTGTCCTTCCCTCTTTTTGCTTCCATTGCTTTCTTTCCAACTAGAACCAAGCACAACAAAACAACCCCACATGATCAAAAATCGCTTCCAAAGTTATTGCCTCTAAACTTGCCAACCAAAGTGGTGATACTTGCCATATTGATCTTAGGAAACAGCTATATTGACCTTGTTTGTCCCAATAATGTAAAGTCATGGATCAATGGTGCTACCGTGTATTTCTACTTAGACGTAGTTATGAGTTTGTCCAATGTCTTTGTTCAATCAACTTTCGGCGTCGAGGTCAGACAACCATTCAACGAGCCATATCTGGCGACATCGCTTCAAAATTTATGGGGCCGGAGGTGGAATCTATTAGTATCTGAAACTTTGCACAGCACCATCTACAAACCAATTCGTTACAAAGTAGGGATGCCACGGTGGGTGGCAGTGGTGACTGTGTTTGTGGTGTCGGGGCTTATGCATGAGCTTTTGTATTACTATATGGTCAGAGCGAATCCGACGTGGGAATTCACGTGGTTCTTTGTGATTCATGGAGTGTGTTTGGCCTTGGAGATTGAATTGAAGAGGGCTTATGAGAAAAAATGGCAGCTACAAAGGGCAGTTTCTACACTATTAACCACGATGTTTACGGGAACCACCACTCTTTGGTTGCTTTTTCCTCTAATGTTGAAGACTTTAGCCTGA

Coding sequence (CDS)

ATGGTTGGCGAAATCGTGAGATTCATCAAGGTGTTGCCAGTATTCATTTTGTCCTCTTTGTATTGCTATTTCATAGCTTCAAAGTTTTCCAAAGCCAAACTCCGGCTTCTCTCTCTCCTCCCTGTCTTCTCCATCTTCGTCGTCCTCCCTCTCTACTTCTCCTCCATCCTCTTCACTAGCAGTGTTGGCTTATTCGTCACCTGGCTTACCACCTTTAAGCTACTTCTTTTCACATTTGATTCGGGTCCTCTAGCTTCCAACCCACCATTGTCCTTCCCTCTTTTTGCTTCCATTGCTTTCTTTCCAACTAGAACCAAGCACAACAAAACAACCCCACATGATCAAAAATCGCTTCCAAAGTTATTGCCTCTAAACTTGCCAACCAAAGTGGTGATACTTGCCATATTGATCTTAGGAAACAGCTATATTGACCTTGTTTGTCCCAATAATGTAAAGTCATGGATCAATGGTGCTACCGTGTATTTCTACTTAGACGTAGTTATGAGTTTGTCCAATGTCTTTGTTCAATCAACTTTCGGCGTCGAGGTCAGACAACCATTCAACGAGCCATATCTGGCGACATCGCTTCAAAATTTATGGGGCCGGAGGTGGAATCTATTAGTATCTGAAACTTTGCACAGCACCATCTACAAACCAATTCGTTACAAAGTAGGGATGCCACGGTGGGTGGCAGTGGTGACTGTGTTTGTGGTGTCGGGGCTTATGCATGAGCTTTTGTATTACTATATGGTCAGAGCGAATCCGACGTGGGAATTCACGTGGTTCTTTGTGATTCATGGAGTGTGTTTGGCCTTGGAGATTGAATTGAAGAGGGCTTATGAGAAAAAATGGCAGCTACAAAGGGCAGTTTCTACACTATTAACCACGATGTTTACGGGAACCACCACTCTTTGGTTGCTTTTTCCTCTAATGTTGAAGACTTTAGCCTGA

Protein sequence

MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTSSVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPKLLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFGVEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTGTTTLWLLFPLMLKTLA*
Homology
BLAST of CSPI07G02130 vs. ExPASy Swiss-Prot
Match: Q9FJ77 (Probable long-chain-alcohol O-fatty-acyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=AT6 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.9e-50
Identity = 119/315 (37.78%), Postives = 176/315 (55.87%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           M  E+  FI+V    I+S  YCY++  K   + LRLLS+LPV  +F+++P++FS++  + 
Sbjct: 1   MEEELKLFIQVWVSAIISVTYCYYLTPKIKTSLLRLLSVLPVCVLFLIIPIFFSTVHSSF 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120
           ++  F++ L   KL+LF  + GPL   PP + P F   A FP + +  K  P +    PK
Sbjct: 61  TIAFFLSGLAVPKLILFALEKGPLFPLPP-NLPHFVCFACFPIKLQ-KKPNPENTNHFPK 120

Query: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180
            +      KV I   L+L   +       N    +    +Y  L++ ++L    V  T G
Sbjct: 121 WV---FALKVFIFGALLLQAYHYKQFLSTNFLLGLYALHIYLELEISLTLIKFLVSITLG 180

Query: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIR-YKVGMPRW---VAVVTVF 240
            ++   FNEPYLATSL + WG RWNL+VS+ L   +Y PIR ++     W    A+   F
Sbjct: 181 CDLEPQFNEPYLATSLHDFWGHRWNLMVSKILWLAVYNPIRQWRAKSSEWDRFFAIFATF 240

Query: 241 VVSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYE--KKWQLQRAVSTLL 300
           +VSG+ HE+LY+Y+ R  PTWE TWFFV+HG C+A E+ LKR  +  ++W +  AVS LL
Sbjct: 241 LVSGVAHEILYFYLTREKPTWEVTWFFVLHGFCMAAEVALKRKTKLVQRWPVNPAVSRLL 300

Query: 301 TTMFTGTTTLWLLFP 310
           T  F   T +WL  P
Sbjct: 301 TVGFVFVTGVWLFSP 310

BLAST of CSPI07G02130 vs. ExPASy Swiss-Prot
Match: Q9FJ73 (Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AT2 PE=3 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.8e-48
Identity = 130/329 (39.51%), Postives = 183/329 (55.62%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           M  E+   IKV    ++S  YCY+I+SK SK  LRLLSLLP+F IF++LPL+FSS+ F  
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120
             G F TWL  FKL LF FD  PL S  P +   F   A FP +   N ++         
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL-SPLPSNLTRFFCFACFPIKINKNPSSNRIH----- 120

Query: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWING------ATVYFYL--DVVMSLSN 180
               N P    +LA  +L  S++  V  NN  S ++        T++ YL  ++++    
Sbjct: 121 ----NKPMSKWVLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVG 180

Query: 181 VFVQSTFGVEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRY----KVGMPR 240
             +    G E+   FN+PYLATSLQ  W RRWNL+V   L   ++ P++      +G+ R
Sbjct: 181 ALMSMLLGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHR 240

Query: 241 --WVAVVTVFVVSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKW-- 300
             +  ++  F+VSGLMHEL+Y+Y++R +PTWE T FF++HGV   LEI +KR    +W  
Sbjct: 241 AFYAGMLATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMKR---MRWLP 300

Query: 301 QLQRAVSTLLTTMFTGTTTLWLLFPLMLK 314
             +RAVS L  T+F   T  WL +P ML+
Sbjct: 301 TPRRAVSGLAITVFLLVTAGWLFYPQMLR 316

BLAST of CSPI07G02130 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 2.4e-48
Identity = 119/319 (37.30%), Postives = 179/319 (56.11%), Query Frame = 0

Query: 4   EIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTSSVG 63
           E+   IKV    I+S  YCY+I  +      R LS+ PV ++F+VLPL+FSS+  +    
Sbjct: 4   ELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITA 63

Query: 64  LFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPKLLP 123
            F+TWL  FKL+LF+FD GPL    P +FP F     FP + + N   P  Q  LPKL+ 
Sbjct: 64  FFLTWLANFKLILFSFDKGPLIP-IPTNFPRFFCFTCFPIKVQQN---PKSQNHLPKLV- 123

Query: 124 LNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFGVEV 183
                K+ I A+L+   SY   + P  +   +    +Y  ++++++   V V  + G ++
Sbjct: 124 --FAIKLAIFAVLLHLYSYRQNLSP-TILLGLYFVHLYLEIEIILTFVKVVVFISLGCDL 183

Query: 184 RQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIR------YKVGMPRWVAVVTVFV 243
               N+PYLATSLQ+ WGRRWNL+V   L   +Y P+R         G   +  ++  F+
Sbjct: 184 EPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFI 243

Query: 244 VSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKR--AYEKKWQLQRAVSTLLT 303
           VSGL+HELL++Y++R  PT E T FFV+HGVC A+E+ +K+     ++W+L   VS +LT
Sbjct: 244 VSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRVLT 303

Query: 304 TMFTGTTTLWLLFPLMLKT 315
             F   T  WL  P + ++
Sbjct: 304 VGFVFVTGGWLFTPQLKRS 314

BLAST of CSPI07G02130 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 4.0e-48
Identity = 126/311 (40.51%), Postives = 176/311 (56.59%), Query Frame = 0

Query: 16  ILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTSSVGLFVTWLTTFKLL 75
           ++S  YCY+I+SK SK  LRLLS+LPV  +F+VLPL+ S + F +   LF++WL  FKLL
Sbjct: 16  LISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLSWLANFKLL 75

Query: 76  LFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPKLLPLNLPTKVVILAI 135
           LF FD GPL   PP     F   A  P + + + +      ++P L P  +P  V+ + I
Sbjct: 76  LFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPS----PNAIPNLHPKPMPKWVLAVKI 135

Query: 136 LILG-----NSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFGVEVRQPFNEP 195
           L+LG       Y D + P  V   +    +Y  +++V+      V +  G  +   FNEP
Sbjct: 136 LVLGVLLHVYEYRDGL-PRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIEPVFNEP 195

Query: 196 YLATSLQNLWGRRWNLLVSETLHSTIYKPIR---YKVGMP---RWVAVVTVFVVSGLMHE 255
           YLATSLQ+ W RRWNL+VS  L ST++ P++    ++  P    +  V+  F VSGLMHE
Sbjct: 196 YLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFVSGLMHE 255

Query: 256 LLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKW--QLQRAVSTLLTTMFTGTT 314
           LLY+YM+R  PTWE T FFV+HG   A EI +KR    +W     RAVS L+   F   T
Sbjct: 256 LLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT---QWLRPPHRAVSGLVVLTFVSVT 315

BLAST of CSPI07G02130 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 5.3e-48
Identity = 115/312 (36.86%), Postives = 170/312 (54.49%), Query Frame = 0

Query: 8   FIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTSSVGLFVT 67
           FIK   + I+S  Y +FIA    K   RL+   PVF IF ++P    S+        F+ 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLTTFKLLLFTFDSGPLASN-PPLSFPLFASIAFFPTR----TKHNKTTPHDQKSLPKLL 127
           WL  FKLLLF    GPL+SN  PLS P+F +++  P +     K  KT  H+  +     
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEG--- 123

Query: 128 PLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFGVE 187
           PL    K V + ++I    Y     P  V   +    +YF L+++++ +   V++   +E
Sbjct: 124 PLIYTIKAVFVVLIIKAYEY-STKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLE 183

Query: 188 VRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPI--RYKVGMPRW---VAVVTVFV 247
           +   FN+PYLATSLQ+ WGRRWNL+V+  L  T+Y+P    + V  P +   +A    FV
Sbjct: 184 LEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLGPNYSQILAAFGTFV 243

Query: 248 VSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTM 307
           VSG+MHEL+++YM R  P W+  WFF+I+G C  +EI +K+    +W+  +A+S +LT  
Sbjct: 244 VSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLT 303

Query: 308 FTGTTTLWLLFP 310
           F   T LWL  P
Sbjct: 304 FVMVTALWLFLP 311

BLAST of CSPI07G02130 vs. ExPASy TrEMBL
Match: A0A0A0K6W1 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G091220 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 5.4e-173
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS
Sbjct: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120
           SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK
Sbjct: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120

Query: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180
           LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG
Sbjct: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180

Query: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240
           VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG
Sbjct: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240

Query: 241 LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG 300
           LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG
Sbjct: 241 LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG 300

Query: 301 TTTLWLLFPLMLKTLA 317
           TTTLWLLFPLMLKTLA
Sbjct: 301 TTTLWLLFPLMLKTLA 316

BLAST of CSPI07G02130 vs. ExPASy TrEMBL
Match: A0A5D3DHE7 (Putative long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G00060 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 4.9e-158
Identity = 289/316 (91.46%), Postives = 303/316 (95.89%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           MV EI+RFI VLP+FILSSLYCYF+ASKFSKAKLRLLSLLPVFSIF+VLPLYFSSILFTS
Sbjct: 1   MVAEIMRFIMVLPIFILSSLYCYFVASKFSKAKLRLLSLLPVFSIFLVLPLYFSSILFTS 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120
           SVGLFVTWLTTFKLLLFTFD+GPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQ+SLPK
Sbjct: 61  SVGLFVTWLTTFKLLLFTFDTGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQESLPK 120

Query: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180
           LL L+LPTKVVILAILILGNSYIDLVCPN VKSWIN AT+YFYLDVVMSLSNVFV+STFG
Sbjct: 121 LLHLDLPTKVVILAILILGNSYIDLVCPNYVKSWINCATMYFYLDVVMSLSNVFVRSTFG 180

Query: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240
           VEVRQPFNEPYLATSLQNLWGRRWNL+VSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG
Sbjct: 181 VEVRQPFNEPYLATSLQNLWGRRWNLIVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240

Query: 241 LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG 300
           LMHELLYYY+VRA PTWEF WFFVIHGVCLALEIELK+AYEK+WQLQ A STLLTT FT 
Sbjct: 241 LMHELLYYYIVRAIPTWEFMWFFVIHGVCLALEIELKKAYEKRWQLQLAASTLLTTTFTA 300

Query: 301 TTTLWLLFPLMLKTLA 317
           TTTLW LFP ML+TLA
Sbjct: 301 TTTLWFLFPPMLRTLA 316

BLAST of CSPI07G02130 vs. ExPASy TrEMBL
Match: A0A1S3BIM7 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo OX=3656 GN=LOC103490115 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.2e-157
Identity = 288/316 (91.14%), Postives = 302/316 (95.57%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           MV EI+RFI VLP+FILSSLYCYF+ASKFSKAKLRLLSLLPVFSIF+VLPLYFSSILFTS
Sbjct: 1   MVAEIMRFIMVLPIFILSSLYCYFVASKFSKAKLRLLSLLPVFSIFLVLPLYFSSILFTS 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120
           SVGLFVTWLTTFKLLL TFD+GPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQ+SLPK
Sbjct: 61  SVGLFVTWLTTFKLLLVTFDTGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQESLPK 120

Query: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180
           LL L+LPTKVVILAILILGNSYIDLVCPN VKSWIN AT+YFYLDVVMSLSNVFV+STFG
Sbjct: 121 LLHLDLPTKVVILAILILGNSYIDLVCPNYVKSWINCATMYFYLDVVMSLSNVFVRSTFG 180

Query: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240
           VEVRQPFNEPYLATSLQNLWGRRWNL+VSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG
Sbjct: 181 VEVRQPFNEPYLATSLQNLWGRRWNLIVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240

Query: 241 LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG 300
           LMHELLYYY+VRA PTWEF WFFVIHGVCLALEIELK+AYEK+WQLQ A STLLTT FT 
Sbjct: 241 LMHELLYYYIVRAIPTWEFMWFFVIHGVCLALEIELKKAYEKRWQLQLAASTLLTTTFTA 300

Query: 301 TTTLWLLFPLMLKTLA 317
           TTTLW LFP ML+TLA
Sbjct: 301 TTTLWFLFPPMLRTLA 316

BLAST of CSPI07G02130 vs. ExPASy TrEMBL
Match: A0A6J1ILW9 (probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111476789 PE=3 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 2.8e-100
Identity = 194/320 (60.62%), Postives = 246/320 (76.88%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           M  E++ F+KVL + ++S LYCYFIA K SK K RL+S LPVFS+F+VLPLY SS   +S
Sbjct: 1   MGSELISFMKVLLISMVSLLYCYFIAQKLSKGKFRLISFLPVFSLFLVLPLYVSSAFLSS 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQ----- 120
           ++  FVTWLTTFKL+LF+F+ GPLA +P LS  LF SIAF PTR KHNKT  + Q     
Sbjct: 61  NISFFVTWLTTFKLILFSFNLGPLAFDPKLSLSLFVSIAFLPTRIKHNKTAKYVQNHDDL 120

Query: 121 --KSLPKLLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNV 180
             +  PK LPL+LP KVVI A+L++G  ++++  P   K ++N AT+YFYLD++MSLSNV
Sbjct: 121 GNEPRPK-LPLSLPAKVVIFAMLVMGGRHLEIGGP-IAKLYLNCATLYFYLDMIMSLSNV 180

Query: 181 FVQSTFGVEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVV 240
           FV+S FGVEVR+PF+EPYLATSLQN WGRRWN  VSETLH+ +Y+P+RY++G+PRW+AVV
Sbjct: 181 FVRSRFGVEVRRPFDEPYLATSLQNFWGRRWNQSVSETLHTAVYQPVRYEIGVPRWMAVV 240

Query: 241 TVFVVSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTL 300
            VFVVSGLMHELL YY++RA PTWE TWFFV HG+C+A+EIELKRA  K+W++  AVS  
Sbjct: 241 VVFVVSGLMHELLLYYIIRAIPTWEITWFFVAHGLCVAVEIELKRALGKRWRIHWAVSRP 300

Query: 301 LTTMFTGTTTLWLLFPLMLK 314
           LT  F   T  WL FP +L+
Sbjct: 301 LTIAFLVATIHWLFFPPLLR 318

BLAST of CSPI07G02130 vs. ExPASy TrEMBL
Match: A0A6J1IJ93 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=3661 GN=LOC111476790 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 1.0e-99
Identity = 196/319 (61.44%), Postives = 240/319 (75.24%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           M  E++  +KV+ + + S LYCYFIA K  K K RL+S LPVFS+F+VLPLY SS   +S
Sbjct: 1   MGSELISLMKVILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSS 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQ----- 120
           S    VTWLTTFKL+LF+F+ GPLA +  LSFPLFASIAF PTR KH+KT  + Q     
Sbjct: 61  SFAFIVTWLTTFKLVLFSFNLGPLAFDSKLSFPLFASIAFLPTRIKHSKTAKYVQNQEEV 120

Query: 121 -KSLPKLLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVF 180
            K     +PLNLP KVVI  +LI+G  Y ++V P   KS +N A +YFYLD++M LSNVF
Sbjct: 121 GKEPHPKIPLNLPAKVVIFVMLIMGKKYYEIVDP-IAKSCMNCAMLYFYLDMIMCLSNVF 180

Query: 181 VQSTFGVEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVT 240
           V+S FGVEV +PFNEPYLATSLQN WGRRWN LVSETLH+ +Y+P+RY+VG PRW+AVV 
Sbjct: 181 VRSRFGVEVSRPFNEPYLATSLQNFWGRRWNRLVSETLHNAVYQPVRYEVGGPRWMAVVV 240

Query: 241 VFVVSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLL 300
           VFVVSGLMHELL YY++R  PTWE TWFFVIHG+C+A+EIELKRA  K+W++  AVS  L
Sbjct: 241 VFVVSGLMHELLLYYIIRVIPTWEVTWFFVIHGLCVAVEIELKRALGKRWRIHWAVSGPL 300

Query: 301 TTMFTGTTTLWLLFPLMLK 314
           TT+F   T  WLLFP +L+
Sbjct: 301 TTVFLVATAHWLLFPPLLR 318

BLAST of CSPI07G02130 vs. NCBI nr
Match: XP_004140072.1 (probable long-chain-alcohol O-fatty-acyltransferase 6 [Cucumis sativus] >KGN44002.1 hypothetical protein Csa_015964 [Cucumis sativus])

HSP 1 Score: 616.7 bits (1589), Expect = 1.1e-172
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS
Sbjct: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120
           SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK
Sbjct: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120

Query: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180
           LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG
Sbjct: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180

Query: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240
           VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG
Sbjct: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240

Query: 241 LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG 300
           LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG
Sbjct: 241 LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG 300

Query: 301 TTTLWLLFPLMLKTLA 317
           TTTLWLLFPLMLKTLA
Sbjct: 301 TTTLWLLFPLMLKTLA 316

BLAST of CSPI07G02130 vs. NCBI nr
Match: KAA0048128.1 (putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa] >TYK23044.1 putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 567.0 bits (1460), Expect = 1.0e-157
Identity = 289/316 (91.46%), Postives = 303/316 (95.89%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           MV EI+RFI VLP+FILSSLYCYF+ASKFSKAKLRLLSLLPVFSIF+VLPLYFSSILFTS
Sbjct: 1   MVAEIMRFIMVLPIFILSSLYCYFVASKFSKAKLRLLSLLPVFSIFLVLPLYFSSILFTS 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120
           SVGLFVTWLTTFKLLLFTFD+GPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQ+SLPK
Sbjct: 61  SVGLFVTWLTTFKLLLFTFDTGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQESLPK 120

Query: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180
           LL L+LPTKVVILAILILGNSYIDLVCPN VKSWIN AT+YFYLDVVMSLSNVFV+STFG
Sbjct: 121 LLHLDLPTKVVILAILILGNSYIDLVCPNYVKSWINCATMYFYLDVVMSLSNVFVRSTFG 180

Query: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240
           VEVRQPFNEPYLATSLQNLWGRRWNL+VSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG
Sbjct: 181 VEVRQPFNEPYLATSLQNLWGRRWNLIVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240

Query: 241 LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG 300
           LMHELLYYY+VRA PTWEF WFFVIHGVCLALEIELK+AYEK+WQLQ A STLLTT FT 
Sbjct: 241 LMHELLYYYIVRAIPTWEFMWFFVIHGVCLALEIELKKAYEKRWQLQLAASTLLTTTFTA 300

Query: 301 TTTLWLLFPLMLKTLA 317
           TTTLW LFP ML+TLA
Sbjct: 301 TTTLWFLFPPMLRTLA 316

BLAST of CSPI07G02130 vs. NCBI nr
Match: XP_008447704.1 (PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo])

HSP 1 Score: 564.3 bits (1453), Expect = 6.6e-157
Identity = 288/316 (91.14%), Postives = 302/316 (95.57%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           MV EI+RFI VLP+FILSSLYCYF+ASKFSKAKLRLLSLLPVFSIF+VLPLYFSSILFTS
Sbjct: 1   MVAEIMRFIMVLPIFILSSLYCYFVASKFSKAKLRLLSLLPVFSIFLVLPLYFSSILFTS 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120
           SVGLFVTWLTTFKLLL TFD+GPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQ+SLPK
Sbjct: 61  SVGLFVTWLTTFKLLLVTFDTGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQESLPK 120

Query: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180
           LL L+LPTKVVILAILILGNSYIDLVCPN VKSWIN AT+YFYLDVVMSLSNVFV+STFG
Sbjct: 121 LLHLDLPTKVVILAILILGNSYIDLVCPNYVKSWINCATMYFYLDVVMSLSNVFVRSTFG 180

Query: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240
           VEVRQPFNEPYLATSLQNLWGRRWNL+VSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG
Sbjct: 181 VEVRQPFNEPYLATSLQNLWGRRWNLIVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240

Query: 241 LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG 300
           LMHELLYYY+VRA PTWEF WFFVIHGVCLALEIELK+AYEK+WQLQ A STLLTT FT 
Sbjct: 241 LMHELLYYYIVRAIPTWEFMWFFVIHGVCLALEIELKKAYEKRWQLQLAASTLLTTTFTA 300

Query: 301 TTTLWLLFPLMLKTLA 317
           TTTLW LFP ML+TLA
Sbjct: 301 TTTLWFLFPPMLRTLA 316

BLAST of CSPI07G02130 vs. NCBI nr
Match: XP_038897379.1 (LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 1 [Benincasa hispida])

HSP 1 Score: 493.4 bits (1269), Expect = 1.4e-135
Identity = 253/314 (80.57%), Postives = 280/314 (89.17%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           M GEI+RFIK+LP+FI+SSLYCYFIASKFSK KLRLLSLLPVFS+F VLPLYFSSILFTS
Sbjct: 1   MGGEIIRFIKMLPIFIMSSLYCYFIASKFSKGKLRLLSLLPVFSLFTVLPLYFSSILFTS 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120
           ++ LFVTWLTTFKLLLF+FD GPLAS+PPLSFPLFASIAFFPTR KHNKTTP DQ+  PK
Sbjct: 61  NIALFVTWLTTFKLLLFSFDLGPLASDPPLSFPLFASIAFFPTRVKHNKTTPPDQEPPPK 120

Query: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180
            LPLNLP+KVVILAILILGNSYI LVCPNNVK WIN  T+YFYLDV+MSLSNVFV+STFG
Sbjct: 121 -LPLNLPSKVVILAILILGNSYIVLVCPNNVKFWINCVTIYFYLDVIMSLSNVFVRSTFG 180

Query: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240
           VEVRQ FNEPYL+TSLQNLWGRRWNLLVSETLH+TIYKP+RYKVGMPRWVA         
Sbjct: 181 VEVRQAFNEPYLSTSLQNLWGRRWNLLVSETLHNTIYKPVRYKVGMPRWVA-------XE 240

Query: 241 LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG 300
           L++ELLYYY+VRA+PTWE TWFFV+HGVCLA+EIELKRAY K+W L  AVS  LTT+F  
Sbjct: 241 LLYELLYYYIVRASPTWEATWFFVMHGVCLAVEIELKRAYGKRWPLHWAVSGPLTTVFMV 300

Query: 301 TTTLWLLFPLMLKT 315
           TTT WLLFPLML++
Sbjct: 301 TTTYWLLFPLMLRS 306

BLAST of CSPI07G02130 vs. NCBI nr
Match: XP_038899290.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Benincasa hispida])

HSP 1 Score: 393.7 bits (1010), Expect = 1.5e-105
Identity = 210/327 (64.22%), Postives = 245/327 (74.92%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           M GEI+RFIKVL + + S LYCY IASK  K KLRLLSLLP+F +FVVLPL  S ++ TS
Sbjct: 1   MDGEIIRFIKVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFPLFVVLPLDLSYVVLTS 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPH------- 120
            + +FVTWLTTFKLL F+FD GPLAS+PPLSFPLFASIAF PTR K  KTTP        
Sbjct: 61  GITIFVTWLTTFKLLQFSFDLGPLASDPPLSFPLFASIAFLPTRIKDIKTTPSVQNHRKD 120

Query: 121 -------DQKSLPKLLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDV 180
                  DQ+  PK LPLNLP KV + A+L+ G SY+DLV P  VK   N   +YFYLD+
Sbjct: 121 SNTPQELDQEPPPK-LPLNLPAKVGLFALLVFGKSYVDLVSP-IVKLCFNCGILYFYLDM 180

Query: 181 VMSLSNVFVQSTFGVEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGM 240
           VMSLSN+FV+S+FGVEVR+ F+EPYLATSLQN WGRRWN LVSETLH+TIYKP+RY VG 
Sbjct: 181 VMSLSNIFVRSSFGVEVRRSFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYDVGS 240

Query: 241 PRWVAVVTVFVVSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQL 300
           PRW+AV  VFVVSGLMHELL+YY++R  PTWE TWFF++HGVC+A EIELK A  KKW  
Sbjct: 241 PRWMAVFVVFVVSGLMHELLFYYVIRVVPTWEVTWFFIVHGVCVAAEIELKSALGKKWHF 300

Query: 301 QRAVSTLLTTMFTGTTTLWLLFPLMLK 314
             AV+  LT  F G T  WL FP +L+
Sbjct: 301 HGAVTGPLTMAFMGVTAHWLFFPQLLR 325

BLAST of CSPI07G02130 vs. TAIR 10
Match: AT5G55330.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 201.1 bits (510), Expect = 1.4e-51
Identity = 119/315 (37.78%), Postives = 176/315 (55.87%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           M  E+  FI+V    I+S  YCY++  K   + LRLLS+LPV  +F+++P++FS++  + 
Sbjct: 1   MEEELKLFIQVWVSAIISVTYCYYLTPKIKTSLLRLLSVLPVCVLFLIIPIFFSTVHSSF 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120
           ++  F++ L   KL+LF  + GPL   PP + P F   A FP + +  K  P +    PK
Sbjct: 61  TIAFFLSGLAVPKLILFALEKGPLFPLPP-NLPHFVCFACFPIKLQ-KKPNPENTNHFPK 120

Query: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180
            +      KV I   L+L   +       N    +    +Y  L++ ++L    V  T G
Sbjct: 121 WV---FALKVFIFGALLLQAYHYKQFLSTNFLLGLYALHIYLELEISLTLIKFLVSITLG 180

Query: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIR-YKVGMPRW---VAVVTVF 240
            ++   FNEPYLATSL + WG RWNL+VS+ L   +Y PIR ++     W    A+   F
Sbjct: 181 CDLEPQFNEPYLATSLHDFWGHRWNLMVSKILWLAVYNPIRQWRAKSSEWDRFFAIFATF 240

Query: 241 VVSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYE--KKWQLQRAVSTLL 300
           +VSG+ HE+LY+Y+ R  PTWE TWFFV+HG C+A E+ LKR  +  ++W +  AVS LL
Sbjct: 241 LVSGVAHEILYFYLTREKPTWEVTWFFVLHGFCMAAEVALKRKTKLVQRWPVNPAVSRLL 300

Query: 301 TTMFTGTTTLWLLFP 310
           T  F   T +WL  P
Sbjct: 301 TVGFVFVTGVWLFSP 310

BLAST of CSPI07G02130 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 194.5 bits (493), Expect = 1.3e-49
Identity = 130/329 (39.51%), Postives = 183/329 (55.62%), Query Frame = 0

Query: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60
           M  E+   IKV    ++S  YCY+I+SK SK  LRLLSLLP+F IF++LPL+FSS+ F  
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120
             G F TWL  FKL LF FD  PL S  P +   F   A FP +   N ++         
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL-SPLPSNLTRFFCFACFPIKINKNPSSNRIH----- 120

Query: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWING------ATVYFYL--DVVMSLSN 180
               N P    +LA  +L  S++  V  NN  S ++        T++ YL  ++++    
Sbjct: 121 ----NKPMSKWVLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVG 180

Query: 181 VFVQSTFGVEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRY----KVGMPR 240
             +    G E+   FN+PYLATSLQ  W RRWNL+V   L   ++ P++      +G+ R
Sbjct: 181 ALMSMLLGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHR 240

Query: 241 --WVAVVTVFVVSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKW-- 300
             +  ++  F+VSGLMHEL+Y+Y++R +PTWE T FF++HGV   LEI +KR    +W  
Sbjct: 241 AFYAGMLATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMKR---MRWLP 300

Query: 301 QLQRAVSTLLTTMFTGTTTLWLLFPLMLK 314
             +RAVS L  T+F   T  WL +P ML+
Sbjct: 301 TPRRAVSGLAITVFLLVTAGWLFYPQMLR 316

BLAST of CSPI07G02130 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 194.1 bits (492), Expect = 1.7e-49
Identity = 119/319 (37.30%), Postives = 179/319 (56.11%), Query Frame = 0

Query: 4   EIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTSSVG 63
           E+   IKV    I+S  YCY+I  +      R LS+ PV ++F+VLPL+FSS+  +    
Sbjct: 4   ELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITA 63

Query: 64  LFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPKLLP 123
            F+TWL  FKL+LF+FD GPL    P +FP F     FP + + N   P  Q  LPKL+ 
Sbjct: 64  FFLTWLANFKLILFSFDKGPLIP-IPTNFPRFFCFTCFPIKVQQN---PKSQNHLPKLV- 123

Query: 124 LNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFGVEV 183
                K+ I A+L+   SY   + P  +   +    +Y  ++++++   V V  + G ++
Sbjct: 124 --FAIKLAIFAVLLHLYSYRQNLSP-TILLGLYFVHLYLEIEIILTFVKVVVFISLGCDL 183

Query: 184 RQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIR------YKVGMPRWVAVVTVFV 243
               N+PYLATSLQ+ WGRRWNL+V   L   +Y P+R         G   +  ++  F+
Sbjct: 184 EPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFI 243

Query: 244 VSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKR--AYEKKWQLQRAVSTLLT 303
           VSGL+HELL++Y++R  PT E T FFV+HGVC A+E+ +K+     ++W+L   VS +LT
Sbjct: 244 VSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRVLT 303

Query: 304 TMFTGTTTLWLLFPLMLKT 315
             F   T  WL  P + ++
Sbjct: 304 VGFVFVTGGWLFTPQLKRS 314

BLAST of CSPI07G02130 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 193.4 bits (490), Expect = 2.9e-49
Identity = 126/311 (40.51%), Postives = 176/311 (56.59%), Query Frame = 0

Query: 16  ILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTSSVGLFVTWLTTFKLL 75
           ++S  YCY+I+SK SK  LRLLS+LPV  +F+VLPL+ S + F +   LF++WL  FKLL
Sbjct: 16  LISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLSWLANFKLL 75

Query: 76  LFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPKLLPLNLPTKVVILAI 135
           LF FD GPL   PP     F   A  P + + + +      ++P L P  +P  V+ + I
Sbjct: 76  LFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPS----PNAIPNLHPKPMPKWVLAVKI 135

Query: 136 LILG-----NSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFGVEVRQPFNEP 195
           L+LG       Y D + P  V   +    +Y  +++V+      V +  G  +   FNEP
Sbjct: 136 LVLGVLLHVYEYRDGL-PRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIEPVFNEP 195

Query: 196 YLATSLQNLWGRRWNLLVSETLHSTIYKPIR---YKVGMP---RWVAVVTVFVVSGLMHE 255
           YLATSLQ+ W RRWNL+VS  L ST++ P++    ++  P    +  V+  F VSGLMHE
Sbjct: 196 YLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFVSGLMHE 255

Query: 256 LLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKW--QLQRAVSTLLTTMFTGTT 314
           LLY+YM+R  PTWE T FFV+HG   A EI +KR    +W     RAVS L+   F   T
Sbjct: 256 LLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT---QWLRPPHRAVSGLVVLTFVSVT 315

BLAST of CSPI07G02130 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 193.0 bits (489), Expect = 3.7e-49
Identity = 115/312 (36.86%), Postives = 170/312 (54.49%), Query Frame = 0

Query: 8   FIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTSSVGLFVT 67
           FIK   + I+S  Y +FIA    K   RL+   PVF IF ++P    S+        F+ 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLTTFKLLLFTFDSGPLASN-PPLSFPLFASIAFFPTR----TKHNKTTPHDQKSLPKLL 127
           WL  FKLLLF    GPL+SN  PLS P+F +++  P +     K  KT  H+  +     
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEG--- 123

Query: 128 PLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFGVE 187
           PL    K V + ++I    Y     P  V   +    +YF L+++++ +   V++   +E
Sbjct: 124 PLIYTIKAVFVVLIIKAYEY-STKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLE 183

Query: 188 VRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPI--RYKVGMPRW---VAVVTVFV 247
           +   FN+PYLATSLQ+ WGRRWNL+V+  L  T+Y+P    + V  P +   +A    FV
Sbjct: 184 LEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLGPNYSQILAAFGTFV 243

Query: 248 VSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTM 307
           VSG+MHEL+++YM R  P W+  WFF+I+G C  +EI +K+    +W+  +A+S +LT  
Sbjct: 244 VSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLT 303

Query: 308 FTGTTTLWLLFP 310
           F   T LWL  P
Sbjct: 304 FVMVTALWLFLP 311

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJ771.9e-5037.78Probable long-chain-alcohol O-fatty-acyltransferase 6 OS=Arabidopsis thaliana OX... [more]
Q9FJ731.8e-4839.51Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX... [more]
Q9FJ762.4e-4837.30Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX... [more]
Q9FJ724.0e-4840.51Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX... [more]
Q9SV075.3e-4836.86Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE... [more]
Match NameE-valueIdentityDescription
A0A0A0K6W15.4e-173100.00MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G091220 PE=... [more]
A0A5D3DHE74.9e-15891.46Putative long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo var. makuw... [more]
A0A1S3BIM73.2e-15791.14probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo OX=3656 GN... [more]
A0A6J1ILW92.8e-10060.63probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Cucurbita maxima OX=366... [more]
A0A6J1IJ931.0e-9961.44probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=366... [more]
Match NameE-valueIdentityDescription
XP_004140072.11.1e-172100.00probable long-chain-alcohol O-fatty-acyltransferase 6 [Cucumis sativus] >KGN4400... [more]
KAA0048128.11.0e-15791.46putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa]... [more]
XP_008447704.16.6e-15791.14PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo][more]
XP_038897379.11.4e-13580.57LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 1 [Beni... [more]
XP_038899290.11.5e-10564.22probable long-chain-alcohol O-fatty-acyltransferase 5 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G55330.11.4e-5137.78MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55370.11.3e-4939.51MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55340.11.7e-4937.30MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55380.12.9e-4940.51MBOAT (membrane bound O-acyl transferase) family protein [more]
AT3G51970.13.7e-4936.86acyl-CoA sterol acyl transferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032805Wax synthase domainPFAMPF13813MBOAT_2coord: 186..263
e-value: 2.0E-16
score: 60.0
NoneNo IPR availablePANTHERPTHR31595:SF8(MEMBRANE BOUND O-ACYL TRANSFERASE) FAMILY PROTEIN, PUTATIVE-RELATEDcoord: 1..314
IPR044851Wax synthasePANTHERPTHR31595LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATEDcoord: 1..314

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G02130.1CSPI07G02130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008374 O-acyltransferase activity