CSPI07G01910 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G01910
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionE3 ubiquitin-protein ligase RNF181-like
LocationChr7: 1641874 .. 1642566 (+)
RNA-Seq ExpressionCSPI07G01910
SyntenyCSPI07G01910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTTTGCTATTTCCAATCCCATTCAGTACCGTCTCTACGCCTCCGGCACCGGAGGCAATGGAGTATTACAAGTTAATATAAGCTTTATCCAACAAATCATCTCTGTTTCTCCGCTCACCGGACGTCCCCATGAAGTCCTCCATCAAACTCCAAGGATTCCGTACCACAATGCTCTCTTTTATCTCTCCTTACGCCAACTCCAAAGTCCGTTGTTCTACATCACTCAAATTCTCTCCTCACTCAACATCCGCCCTGCCGCTTCCCAAAACATGGCTCGTCGTATCGCTTCCCACATCCTACAAATGCCTGACAGAAACAGCGAGACGAATTTCCATATCCTCGCTGAAGTGGATTTCATTCGACTCATTTGGCTGCCATCAATGGGTGGAGGCGGTGCAGGGGCGGAGGAAACAGTTGTGTTGGATGAAGCTCCGCCGGCAGTGAAGAGGGGAGTGGGGGTGGCGAGGGGAGAGAGGTTGAGATCGGAAGAAAAGATGGAGGAATTGGGAGATTGCAGCATCTGTTTGGATGAATTGAGTTGTGAAAAGAGGGAGGTGATGAGGATTCCTTGTGGCCATGTTTACCATGAATCGTGCATCTTCAAGTGGCTTGAGAATCACAATTCTTGTCCTTTGTGTAGAAAGCCATTGCACCACGACGAGGAGGAGGAGGAGGAGTACTCATGGTGA

mRNA sequence

ATGTCTTTTGCTATTTCCAATCCCATTCAGTACCGTCTCTACGCCTCCGGCACCGGAGGCAATGGAGTATTACAAGTTAATATAAGCTTTATCCAACAAATCATCTCTGTTTCTCCGCTCACCGGACGTCCCCATGAAGTCCTCCATCAAACTCCAAGGATTCCGTACCACAATGCTCTCTTTTATCTCTCCTTACGCCAACTCCAAAGTCCGTTGTTCTACATCACTCAAATTCTCTCCTCACTCAACATCCGCCCTGCCGCTTCCCAAAACATGGCTCGTCGTATCGCTTCCCACATCCTACAAATGCCTGACAGAAACAGCGAGACGAATTTCCATATCCTCGCTGAAGTGGATTTCATTCGACTCATTTGGCTGCCATCAATGGGTGGAGGCGGTGCAGGGGCGGAGGAAACAGTTGTGTTGGATGAAGCTCCGCCGGCAGTGAAGAGGGGAGTGGGGGTGGCGAGGGGAGAGAGGTTGAGATCGGAAGAAAAGATGGAGGAATTGGGAGATTGCAGCATCTGTTTGGATGAATTGAGTTGTGAAAAGAGGGAGGTGATGAGGATTCCTTGTGGCCATGTTTACCATGAATCGTGCATCTTCAAGTGGCTTGAGAATCACAATTCTTGTCCTTTGTGTAGAAAGCCATTGCACCACGACGAGGAGGAGGAGGAGGAGTACTCATGGTGA

Coding sequence (CDS)

ATGTCTTTTGCTATTTCCAATCCCATTCAGTACCGTCTCTACGCCTCCGGCACCGGAGGCAATGGAGTATTACAAGTTAATATAAGCTTTATCCAACAAATCATCTCTGTTTCTCCGCTCACCGGACGTCCCCATGAAGTCCTCCATCAAACTCCAAGGATTCCGTACCACAATGCTCTCTTTTATCTCTCCTTACGCCAACTCCAAAGTCCGTTGTTCTACATCACTCAAATTCTCTCCTCACTCAACATCCGCCCTGCCGCTTCCCAAAACATGGCTCGTCGTATCGCTTCCCACATCCTACAAATGCCTGACAGAAACAGCGAGACGAATTTCCATATCCTCGCTGAAGTGGATTTCATTCGACTCATTTGGCTGCCATCAATGGGTGGAGGCGGTGCAGGGGCGGAGGAAACAGTTGTGTTGGATGAAGCTCCGCCGGCAGTGAAGAGGGGAGTGGGGGTGGCGAGGGGAGAGAGGTTGAGATCGGAAGAAAAGATGGAGGAATTGGGAGATTGCAGCATCTGTTTGGATGAATTGAGTTGTGAAAAGAGGGAGGTGATGAGGATTCCTTGTGGCCATGTTTACCATGAATCGTGCATCTTCAAGTGGCTTGAGAATCACAATTCTTGTCCTTTGTGTAGAAAGCCATTGCACCACGACGAGGAGGAGGAGGAGGAGTACTCATGGTGA

Protein sequence

MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNALFYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDFIRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDEEEEEEYSW*
Homology
BLAST of CSPI07G01910 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.2e-11
Identity = 30/84 (35.71%), Postives = 48/84 (57.14%), Query Frame = 0

Query: 146 PPAVKRGVGVARGERLRSEEKMEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWL 205
           PPA K  +      ++  +    E+  C++C+DE   +  +V ++PC HV+H+ C+  WL
Sbjct: 189 PPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFE-DGSDVKQMPCKHVFHQDCLLPWL 248

Query: 206 ENHNSCPLCRKPLHHDEEEEEEYS 230
           E HNSCP+CR  L  D+ + E  S
Sbjct: 249 ELHNSCPVCRFELPTDDPDYENRS 271

BLAST of CSPI07G01910 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.9e-10
Identity = 29/81 (35.80%), Postives = 43/81 (53.09%), Query Frame = 0

Query: 146 PPAVKRGVGVARGERLRSEEKMEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWL 205
           PPA K  +       +       E   C++C+D+   E  E  ++PC H+YH+ C+  WL
Sbjct: 197 PPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFE-EGTEAKQMPCKHLYHKDCLLPWL 256

Query: 206 ENHNSCPLCRKPLHHDEEEEE 227
           E HNSCP+CR  L  D+ + E
Sbjct: 257 ELHNSCPVCRHELPTDDPDYE 276

BLAST of CSPI07G01910 vs. ExPASy Swiss-Prot
Match: Q9Y7K6 (Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC2A9.04c PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.2e-10
Identity = 33/90 (36.67%), Postives = 55/90 (61.11%), Query Frame = 0

Query: 135 GAEETVVLDEAPPAVKRGVGVA--RGERLRSEEKMEELGDCSICLDELS-CEKREVMRIP 194
           G E     +   P VKR V  A    E L +++ M+    C IC D+++  ++++  ++P
Sbjct: 69  GGENDPFANPFQPPVKRAVKEAWDSFEPLSNDQLMDL--TCPICYDDMNENDEKQATKMP 128

Query: 195 CGHVYHESCIFKWLENHNSCPLCRKPLHHD 222
           CGH++ ++C+ KWLENH +CPLCRK + H+
Sbjct: 129 CGHIFGKNCLQKWLENHCTCPLCRKEVPHE 156

BLAST of CSPI07G01910 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 7.1e-10
Identity = 36/90 (40.00%), Postives = 51/90 (56.67%), Query Frame = 0

Query: 136 AEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDELSCEKREVMRIPCGHV 195
           AE     +  PPA K  V     E L +  K+EE+  CS+CLD+L     +  ++PC H 
Sbjct: 168 AESDPSRNGTPPAKKEAV-----EALPT-VKIEEVVSCSVCLDDLEVGS-QAKQMPCEHK 227

Query: 196 YHESCIFKWLENHNSCPLCRKPLHHDEEEE 226
           +H SCI  WLE H+SCP+CR  L  +E ++
Sbjct: 228 FHSSCILPWLELHSSCPVCRFELPSEETKD 250

BLAST of CSPI07G01910 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 9.2e-10
Identity = 28/73 (38.36%), Postives = 37/73 (50.68%), Query Frame = 0

Query: 146 PPAVKRGVGVARGERLRSEEKMEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWL 205
           PPA K  +      ++  +        C +C DE    K E  ++PC H+YH  CI  WL
Sbjct: 159 PPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFEL-KSEAKQMPCHHIYHSDCIVPWL 218

Query: 206 ENHNSCPLCRKPL 219
             HNSCP+CRK L
Sbjct: 219 VQHNSCPVCRKEL 230

BLAST of CSPI07G01910 vs. ExPASy TrEMBL
Match: A0A0A0K696 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G026750 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 6.0e-129
Identity = 227/230 (98.70%), Postives = 230/230 (100.00%), Query Frame = 0

Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
           FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120

Query: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL 180
           IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL
Sbjct: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL 180

Query: 181 SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDEEEEEEYSW 231
           SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHD+++EEEYSW
Sbjct: 181 SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEEEYSW 230

BLAST of CSPI07G01910 vs. ExPASy TrEMBL
Match: A0A0A0K2W2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G025210 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 6.0e-129
Identity = 227/230 (98.70%), Postives = 230/230 (100.00%), Query Frame = 0

Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
           FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120

Query: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL 180
           IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL
Sbjct: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL 180

Query: 181 SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDEEEEEEYSW 231
           SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHD+E++EEYSW
Sbjct: 181 SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDEDDEEYSW 230

BLAST of CSPI07G01910 vs. ExPASy TrEMBL
Match: A0A5D3E1S5 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold208G00800 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.8e-104
Identity = 187/230 (81.30%), Postives = 209/230 (90.87%), Query Frame = 0

Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLY SGTGGNG+L+V ISF +Q+IS+SP+TGRPH++LHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNGILEVRISFFEQMISLSPVTGRPHQILHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
            YL+LRQLQSPLFYITQ LSSLNIRPAASQ+MAR+IASHILQMPDRN + NFHI+AEVDF
Sbjct: 61  VYLTLRQLQSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNFHIIAEVDF 120

Query: 121 IRLIWLP-SMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDE 180
           IR+IWL       GAGAEETVV DEAPPA+KRGVG ARGERL+SEEK+E+LGDCSICLDE
Sbjct: 121 IRVIWLELEEQSMGAGAEETVVFDEAPPALKRGVGKARGERLKSEEKVEDLGDCSICLDE 180

Query: 181 LSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDEEEEEEYS 230
           LSCEKREV+RIPCGHVYHESCIFKWL+NHNSCPLCRKPL   E+EE+  S
Sbjct: 181 LSCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPLKEDEEDSSS 230

BLAST of CSPI07G01910 vs. ExPASy TrEMBL
Match: A0A1S4DUZ4 (E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.8e-104
Identity = 187/230 (81.30%), Postives = 209/230 (90.87%), Query Frame = 0

Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLY SGTGGNG+L+V ISF +Q+IS+SP+TGRPH++LHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNGILEVRISFFEQMISLSPVTGRPHQILHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
            YL+LRQLQSPLFYITQ LSSLNIRPAASQ+MAR+IASHILQMPDRN + NFHI+AEVDF
Sbjct: 61  VYLTLRQLQSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNFHIIAEVDF 120

Query: 121 IRLIWLP-SMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDE 180
           IR+IWL       GAGAEETVV DEAPPA+KRGVG ARGERL+SEEK+E+LGDCSICLDE
Sbjct: 121 IRVIWLELEEQSMGAGAEETVVFDEAPPALKRGVGKARGERLKSEEKVEDLGDCSICLDE 180

Query: 181 LSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDEEEEEEYS 230
           LSCEKREV+RIPCGHVYHESCIFKWL+NHNSCPLCRKPL   E+EE+  S
Sbjct: 181 LSCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPLKEDEEDSSS 230

BLAST of CSPI07G01910 vs. ExPASy TrEMBL
Match: A0A5A7TLM1 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001430 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 9.8e-55
Identity = 125/223 (56.05%), Postives = 163/223 (73.09%), Query Frame = 0

Query: 1   MSFAISNPIQYRLYASGTG-GNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNA 60
           MSFAIS+PIQY L  +GTG GNGVL++ +S+++++IS+S ++ R   +L++T  IP++NA
Sbjct: 1   MSFAISSPIQYLL--NGTGFGNGVLEIQLSYVERVISISSVSQR---ILYETQPIPFNNA 60

Query: 61  LFYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNS--ETNFHILAE 120
            FYLS+  LQ PLFYI Q L SLNIR    +N+AR+IAS++  M + N+   +NF+++A 
Sbjct: 61  HFYLSVHHLQDPLFYIRQFLFSLNIR---RENIARQIASYVAHMCNGNTGRNSNFYVIAR 120

Query: 121 VDFIRLIWL--PSM---GGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGD 180
           VD +R I +   SM   GGG     ETVV DE P A +RGV V  GERLR  +  EE GD
Sbjct: 121 VDLVRAIRIEEESMWRDGGGVVVEAETVVFDEVPRA-RRGVVV--GERLRKLKSEEEEGD 180

Query: 181 CSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCR 216
           CS+CLDEL C KREV+RIPCGHVYHESCIF+WL N+NSCPLCR
Sbjct: 181 CSVCLDELDCGKREVIRIPCGHVYHESCIFEWLNNNNSCPLCR 212

BLAST of CSPI07G01910 vs. NCBI nr
Match: XP_004144954.1 (uncharacterized protein LOC101214496 [Cucumis sativus] >KGN43349.1 hypothetical protein Csa_020356 [Cucumis sativus])

HSP 1 Score: 469.9 bits (1208), Expect = 1.2e-128
Identity = 227/230 (98.70%), Postives = 230/230 (100.00%), Query Frame = 0

Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
           FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120

Query: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL 180
           IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL
Sbjct: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL 180

Query: 181 SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDEEEEEEYSW 231
           SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHD+E++EEYSW
Sbjct: 181 SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDEDDEEYSW 230

BLAST of CSPI07G01910 vs. NCBI nr
Match: XP_011659694.1 (E3 ubiquitin-protein ligase RDUF1 [Cucumis sativus] >KGN43356.1 hypothetical protein Csa_020163 [Cucumis sativus])

HSP 1 Score: 469.9 bits (1208), Expect = 1.2e-128
Identity = 227/230 (98.70%), Postives = 230/230 (100.00%), Query Frame = 0

Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
           FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120

Query: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL 180
           IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL
Sbjct: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL 180

Query: 181 SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDEEEEEEYSW 231
           SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHD+++EEEYSW
Sbjct: 181 SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEEEYSW 230

BLAST of CSPI07G01910 vs. NCBI nr
Match: XP_016899530.1 (PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa] >TYK29842.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 388.7 bits (997), Expect = 3.6e-104
Identity = 187/230 (81.30%), Postives = 209/230 (90.87%), Query Frame = 0

Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLY SGTGGNG+L+V ISF +Q+IS+SP+TGRPH++LHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNGILEVRISFFEQMISLSPVTGRPHQILHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
            YL+LRQLQSPLFYITQ LSSLNIRPAASQ+MAR+IASHILQMPDRN + NFHI+AEVDF
Sbjct: 61  VYLTLRQLQSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNFHIIAEVDF 120

Query: 121 IRLIWLP-SMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDE 180
           IR+IWL       GAGAEETVV DEAPPA+KRGVG ARGERL+SEEK+E+LGDCSICLDE
Sbjct: 121 IRVIWLELEEQSMGAGAEETVVFDEAPPALKRGVGKARGERLKSEEKVEDLGDCSICLDE 180

Query: 181 LSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDEEEEEEYS 230
           LSCEKREV+RIPCGHVYHESCIFKWL+NHNSCPLCRKPL   E+EE+  S
Sbjct: 181 LSCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPLKEDEEDSSS 230

BLAST of CSPI07G01910 vs. NCBI nr
Match: XP_038883574.1 (E3 ubiquitin-protein ligase RNF181-like [Benincasa hispida])

HSP 1 Score: 298.5 bits (763), Expect = 4.9e-77
Identity = 149/221 (67.42%), Postives = 179/221 (81.00%), Query Frame = 0

Query: 1   MSFAISNPIQYRLYASGTG-GNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNA 60
           MS A S PIQYRLY +G+G GNGVL+V  S+ +++ISVSP+TGRP  +LH+TPRIP+HNA
Sbjct: 1   MSSAASTPIQYRLYGTGSGTGNGVLEVQTSYFERMISVSPVTGRPPRILHETPRIPFHNA 60

Query: 61  LFYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVD 120
           LFYLSL QLQ+PLFYI+Q LSSLNI PAA + MAR+IAS+I +M + N E+NF I+AEVD
Sbjct: 61  LFYLSLHQLQNPLFYISQFLSSLNICPAACEIMARQIASYIDRMINENRESNFRIIAEVD 120

Query: 121 FIRLIWLP--SMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICL 180
           F R+IW+   S+ GG A AEETVV DEAPP  +RGV     ER+R  +  EELGDC ICL
Sbjct: 121 FSRVIWMEEHSIYGGDAMAEETVVFDEAPPP-RRGVEKVAVERVRELKSQEELGDCCICL 180

Query: 181 DELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPL 219
           +ELSCEKREV+RIPCGHVYHESC+FKWL  +NSCPLCRKPL
Sbjct: 181 EELSCEKREVIRIPCGHVYHESCVFKWLRKNNSCPLCRKPL 220

BLAST of CSPI07G01910 vs. NCBI nr
Match: XP_016901482.1 (PREDICTED: uncharacterized protein LOC107991269 [Cucumis melo] >KAA0044413.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa] >TYK29540.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 223.4 bits (568), Expect = 2.0e-54
Identity = 125/223 (56.05%), Postives = 163/223 (73.09%), Query Frame = 0

Query: 1   MSFAISNPIQYRLYASGTG-GNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNA 60
           MSFAIS+PIQY L  +GTG GNGVL++ +S+++++IS+S ++ R   +L++T  IP++NA
Sbjct: 1   MSFAISSPIQYLL--NGTGFGNGVLEIQLSYVERVISISSVSQR---ILYETQPIPFNNA 60

Query: 61  LFYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNS--ETNFHILAE 120
            FYLS+  LQ PLFYI Q L SLNIR    +N+AR+IAS++  M + N+   +NF+++A 
Sbjct: 61  HFYLSVHHLQDPLFYIRQFLFSLNIR---RENIARQIASYVAHMCNGNTGRNSNFYVIAR 120

Query: 121 VDFIRLIWL--PSM---GGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGD 180
           VD +R I +   SM   GGG     ETVV DE P A +RGV V  GERLR  +  EE GD
Sbjct: 121 VDLVRAIRIEEESMWRDGGGVVVEAETVVFDEVPRA-RRGVVV--GERLRKLKSEEEEGD 180

Query: 181 CSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCR 216
           CS+CLDEL C KREV+RIPCGHVYHESCIF+WL N+NSCPLCR
Sbjct: 181 CSVCLDELDCGKREVIRIPCGHVYHESCIFEWLNNNNSCPLCR 212

BLAST of CSPI07G01910 vs. TAIR 10
Match: AT5G37200.1 (RING/U-box superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.4e-13
Identity = 34/98 (34.69%), Postives = 57/98 (58.16%), Query Frame = 0

Query: 137 EETVVLDEAPPAVKRGVG------VARGERLRSEEKMEELGDCSICLDELSCEKREVMRI 196
           E  V+    P  + RG        +   +R+ S +  EE   CSIC+++ S    +++R+
Sbjct: 117 ERRVIFPHGPSLLSRGASGEVFHRLVEEQRVESADLEEEDETCSICIEKFSESHEDIIRV 176

Query: 197 P-CGHVYHESCIFKWLENHNSCPLCRKPLHHDEEEEEE 228
           P C H++H+ C+F+WL   NSCPLCRK  + +E+E+E+
Sbjct: 177 PDCLHLFHQGCLFEWLGLQNSCPLCRKVPYEEEDEDED 214

BLAST of CSPI07G01910 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.6e-12
Identity = 30/84 (35.71%), Postives = 48/84 (57.14%), Query Frame = 0

Query: 146 PPAVKRGVGVARGERLRSEEKMEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWL 205
           PPA K  +      ++  +    E+  C++C+DE   +  +V ++PC HV+H+ C+  WL
Sbjct: 189 PPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFE-DGSDVKQMPCKHVFHQDCLLPWL 248

Query: 206 ENHNSCPLCRKPLHHDEEEEEEYS 230
           E HNSCP+CR  L  D+ + E  S
Sbjct: 249 ELHNSCPVCRFELPTDDPDYENRS 271

BLAST of CSPI07G01910 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 6.5e-11
Identity = 28/73 (38.36%), Postives = 37/73 (50.68%), Query Frame = 0

Query: 146 PPAVKRGVGVARGERLRSEEKMEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWL 205
           PPA K  +      ++  +        C +C DE    K E  ++PC H+YH  CI  WL
Sbjct: 159 PPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFEL-KSEAKQMPCHHIYHSDCIVPWL 218

Query: 206 ENHNSCPLCRKPL 219
             HNSCP+CRK L
Sbjct: 219 VQHNSCPVCRKEL 230

BLAST of CSPI07G01910 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 6.5e-11
Identity = 28/73 (38.36%), Postives = 37/73 (50.68%), Query Frame = 0

Query: 146 PPAVKRGVGVARGERLRSEEKMEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWL 205
           PPA K  +      ++  +        C +C DE    K E  ++PC H+YH  CI  WL
Sbjct: 159 PPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFEL-KSEAKQMPCHHIYHSDCIVPWL 218

Query: 206 ENHNSCPLCRKPL 219
             HNSCP+CRK L
Sbjct: 219 VQHNSCPVCRKEL 230

BLAST of CSPI07G01910 vs. TAIR 10
Match: AT3G56580.3 (RING/U-box superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 6.5e-11
Identity = 28/73 (38.36%), Postives = 37/73 (50.68%), Query Frame = 0

Query: 146 PPAVKRGVGVARGERLRSEEKMEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWL 205
           PPA K  +      ++  +        C +C DE    K E  ++PC H+YH  CI  WL
Sbjct: 159 PPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFEL-KSEAKQMPCHHIYHSDCIVPWL 218

Query: 206 ENHNSCPLCRKPL 219
             HNSCP+CRK L
Sbjct: 219 VQHNSCPVCRKEL 230

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LPN72.2e-1135.71E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
P0CH301.9e-1035.80E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q9Y7K63.2e-1036.67Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strai... [more]
Q6AVN27.1e-1040.00E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI... [more]
Q94AK49.2e-1038.36E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A0A0K6966.0e-12998.70RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G026750 P... [more]
A0A0A0K2W26.0e-12998.70RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G025210 P... [more]
A0A5D3E1S51.8e-10481.30E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S4DUZ41.8e-10481.30E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 ... [more]
A0A5A7TLM19.8e-5556.05E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1... [more]
Match NameE-valueIdentityDescription
XP_004144954.11.2e-12898.70uncharacterized protein LOC101214496 [Cucumis sativus] >KGN43349.1 hypothetical ... [more]
XP_011659694.11.2e-12898.70E3 ubiquitin-protein ligase RDUF1 [Cucumis sativus] >KGN43356.1 hypothetical pro... [more]
XP_016899530.13.6e-10481.30PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 ... [more]
XP_038883574.14.9e-7767.42E3 ubiquitin-protein ligase RNF181-like [Benincasa hispida][more]
XP_016901482.12.0e-5456.05PREDICTED: uncharacterized protein LOC107991269 [Cucumis melo] >KAA0044413.1 E3 ... [more]
Match NameE-valueIdentityDescription
AT5G37200.11.4e-1334.69RING/U-box superfamily protein [more]
AT3G19950.11.6e-1235.71RING/U-box superfamily protein [more]
AT3G56580.26.5e-1138.36RING/U-box superfamily protein [more]
AT3G56580.16.5e-1138.36RING/U-box superfamily protein [more]
AT3G56580.36.5e-1138.36RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 173..214
e-value: 4.4E-8
score: 42.9
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 172..215
e-value: 2.2E-13
score: 50.3
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 173..215
score: 13.477729
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 158..229
e-value: 1.6E-19
score: 70.9
NoneNo IPR availablePANTHERPTHR22765:SF329SUBFAMILY NOT NAMEDcoord: 4..216
NoneNo IPR availablePANTHERPTHR22765RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAININGcoord: 4..216
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 172..221

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G01910.1CSPI07G01910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0061630 ubiquitin protein ligase activity