CSPI07G01850 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G01850
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	E3 ubiquitin-protein ligase RNF181-like
Location	Chr7: 1605596 .. 1606078 (-)
RNA-Seq Expression	CSPI07G01850
Synteny	CSPI07G01850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTTTGCTATTTCCAATCCCATTCAGTACCGTCTCTACGCCTCCGGCACCGGAGGCAATGGAGTATTACAAGTTAATATAAGCTTTATCCAACAAATCATCTCTGTTTCTCCGCTCACCGGACGTCCCCATGAAGTCCTCCATCAAACTCCAAGGATTCCGTACCACAATGCTCTCTTTTATCTCTCCTTACGCCAACTCCAAAGTCCGTTGTTCTACATCACTCAAATTCTCTCCTCACTCAACATCCGCCCTGCCGCTTCCCAAAACATGGCTCGTCGTATCGCTTCCCACATCCTACAAATGCCTGACAGAAACAGCGAGACGAATTTCCATATCCTCGCTGAAGTGGATTTCATTCGACTCATTTGGCTGCCATCAATGGGTGGAGGCGGTGCAGGGGCGGAGGAAACAGTTGTGTTGGATGAAGCTCCGCCGGCAGTGAAGAGGGGAGTGGGGGTGGCGAGGGGAGAGAGGTAG

mRNA sequence

ATGTCTTTTGCTATTTCCAATCCCATTCAGTACCGTCTCTACGCCTCCGGCACCGGAGGCAATGGAGTATTACAAGTTAATATAAGCTTTATCCAACAAATCATCTCTGTTTCTCCGCTCACCGGACGTCCCCATGAAGTCCTCCATCAAACTCCAAGGATTCCGTACCACAATGCTCTCTTTTATCTCTCCTTACGCCAACTCCAAAGTCCGTTGTTCTACATCACTCAAATTCTCTCCTCACTCAACATCCGCCCTGCCGCTTCCCAAAACATGGCTCGTCGTATCGCTTCCCACATCCTACAAATGCCTGACAGAAACAGCGAGACGAATTTCCATATCCTCGCTGAAGTGGATTTCATTCGACTCATTTGGCTGCCATCAATGGGTGGAGGCGGTGCAGGGGCGGAGGAAACAGTTGTGTTGGATGAAGCTCCGCCGGCAGTGAAGAGGGGAGTGGGGGTGGCGAGGGGAGAGAGGTAG

Coding sequence (CDS)

ATGTCTTTTGCTATTTCCAATCCCATTCAGTACCGTCTCTACGCCTCCGGCACCGGAGGCAATGGAGTATTACAAGTTAATATAAGCTTTATCCAACAAATCATCTCTGTTTCTCCGCTCACCGGACGTCCCCATGAAGTCCTCCATCAAACTCCAAGGATTCCGTACCACAATGCTCTCTTTTATCTCTCCTTACGCCAACTCCAAAGTCCGTTGTTCTACATCACTCAAATTCTCTCCTCACTCAACATCCGCCCTGCCGCTTCCCAAAACATGGCTCGTCGTATCGCTTCCCACATCCTACAAATGCCTGACAGAAACAGCGAGACGAATTTCCATATCCTCGCTGAAGTGGATTTCATTCGACTCATTTGGCTGCCATCAATGGGTGGAGGCGGTGCAGGGGCGGAGGAAACAGTTGTGTTGGATGAAGCTCCGCCGGCAGTGAAGAGGGGAGTGGGGGTGGCGAGGGGAGAGAGGTAG

Protein sequence

MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNALFYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDFIRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER*

Homology

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BLAST of CSPI07G01850 vs. ExPASy TrEMBL
Match: A0A0A0K696 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G026750 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.6e-83
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
           FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120

Query: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER 161
           IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER
Sbjct: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER 160

[Close]

BLAST of CSPI07G01850 vs. ExPASy TrEMBL
Match: A0A0A0K2W2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G025210 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.6e-83
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
           FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120

Query: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER 161
           IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER
Sbjct: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER 160

[Close]

BLAST of CSPI07G01850 vs. ExPASy TrEMBL
Match: A0A5D3E1S5 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold208G00800 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.5e-67
Identity = 130/161 (80.75%), Postives = 145/161 (90.06%), Query Frame = 0


Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLY SGTGGNG+L+V ISF +Q+IS+SP+TGRPH++LHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNGILEVRISFFEQMISLSPVTGRPHQILHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
            YL+LRQLQSPLFYITQ LSSLNIRPAASQ+MAR+IASHILQMPDRN + NFHI+AEVDF
Sbjct: 61  VYLTLRQLQSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNFHIIAEVDF 120

Query: 121 IRLIWLP-SMGGGGAGAEETVVLDEAPPAVKRGVGVARGER 161
           IR+IWL       GAGAEETVV DEAPPA+KRGVG ARGER
Sbjct: 121 IRVIWLELEEQSMGAGAEETVVFDEAPPALKRGVGKARGER 161

[Close]

BLAST of CSPI07G01850 vs. ExPASy TrEMBL
Match: A0A1S4DUZ4 (E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.5e-67
Identity = 130/161 (80.75%), Postives = 145/161 (90.06%), Query Frame = 0


Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLY SGTGGNG+L+V ISF +Q+IS+SP+TGRPH++LHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNGILEVRISFFEQMISLSPVTGRPHQILHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
            YL+LRQLQSPLFYITQ LSSLNIRPAASQ+MAR+IASHILQMPDRN + NFHI+AEVDF
Sbjct: 61  VYLTLRQLQSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNFHIIAEVDF 120

Query: 121 IRLIWLP-SMGGGGAGAEETVVLDEAPPAVKRGVGVARGER 161
           IR+IWL       GAGAEETVV DEAPPA+KRGVG ARGER
Sbjct: 121 IRVIWLELEEQSMGAGAEETVVFDEAPPALKRGVGKARGER 161

[Close]

BLAST of CSPI07G01850 vs. ExPASy TrEMBL
Match: A0A5A7TLM1 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001430 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.9e-26
Identity = 83/168 (49.40%), Postives = 116/168 (69.05%), Query Frame = 0


Query: 1   MSFAISNPIQYRLYASGTG-GNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNA 60
           MSFAIS+PIQY L  +GTG GNGVL++ +S+++++IS+S ++ R   +L++T  IP++NA
Sbjct: 1   MSFAISSPIQYLL--NGTGFGNGVLEIQLSYVERVISISSVSQR---ILYETQPIPFNNA 60

Query: 61  LFYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNS--ETNFHILAE 120
            FYLS+  LQ PLFYI Q L SLNIR    +N+AR+IAS++  M + N+   +NF+++A 
Sbjct: 61  HFYLSVHHLQDPLFYIRQFLFSLNIR---RENIARQIASYVAHMCNGNTGRNSNFYVIAR 120

Query: 121 VDFIRLIWL--PSM---GGGGAGAEETVVLDEAPPAVKRGVGVARGER 161
           VD +R I +   SM   GGG     ETVV DE P A +RGV V  GER
Sbjct: 121 VDLVRAIRIEEESMWRDGGGVVVEAETVVFDEVPRA-RRGVVV--GER 157

[Close]

BLAST of CSPI07G01850 vs. NCBI nr
Match: XP_004144954.1 (uncharacterized protein LOC101214496 [Cucumis sativus] >KGN43349.1 hypothetical protein Csa_020356 [Cucumis sativus])

HSP 1 Score: 318.5 bits (815), Expect = 3.2e-83
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
           FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120

Query: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER 161
           IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER
Sbjct: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER 160

[Close]

BLAST of CSPI07G01850 vs. NCBI nr
Match: XP_011659694.1 (E3 ubiquitin-protein ligase RDUF1 [Cucumis sativus] >KGN43356.1 hypothetical protein Csa_020163 [Cucumis sativus])

HSP 1 Score: 318.5 bits (815), Expect = 3.2e-83
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
           FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120

Query: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER 161
           IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER
Sbjct: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGER 160

[Close]

BLAST of CSPI07G01850 vs. NCBI nr
Match: XP_016899530.1 (PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa] >TYK29842.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 263.8 bits (673), Expect = 9.4e-67
Identity = 130/161 (80.75%), Postives = 145/161 (90.06%), Query Frame = 0


Query: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60
           MSFAISNPIQYRLY SGTGGNG+L+V ISF +Q+IS+SP+TGRPH++LHQTPRIPYHNAL
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNGILEVRISFFEQMISLSPVTGRPHQILHQTPRIPYHNAL 60

Query: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120
            YL+LRQLQSPLFYITQ LSSLNIRPAASQ+MAR+IASHILQMPDRN + NFHI+AEVDF
Sbjct: 61  VYLTLRQLQSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNFHIIAEVDF 120

Query: 121 IRLIWLP-SMGGGGAGAEETVVLDEAPPAVKRGVGVARGER 161
           IR+IWL       GAGAEETVV DEAPPA+KRGVG ARGER
Sbjct: 121 IRVIWLELEEQSMGAGAEETVVFDEAPPALKRGVGKARGER 161

[Close]

BLAST of CSPI07G01850 vs. NCBI nr
Match: XP_038883574.1 (E3 ubiquitin-protein ligase RNF181-like [Benincasa hispida])

HSP 1 Score: 194.1 bits (492), Expect = 9.1e-46
Identity = 104/163 (63.80%), Postives = 128/163 (78.53%), Query Frame = 0


Query: 1   MSFAISNPIQYRLYASGTG-GNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNA 60
           MS A S PIQYRLY +G+G GNGVL+V  S+ +++ISVSP+TGRP  +LH+TPRIP+HNA
Sbjct: 1   MSSAASTPIQYRLYGTGSGTGNGVLEVQTSYFERMISVSPVTGRPPRILHETPRIPFHNA 60

Query: 61  LFYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVD 120
           LFYLSL QLQ+PLFYI+Q LSSLNI PAA + MAR+IAS+I +M + N E+NF I+AEVD
Sbjct: 61  LFYLSLHQLQNPLFYISQFLSSLNICPAACEIMARQIASYIDRMINENRESNFRIIAEVD 120

Query: 121 FIRLIWLP--SMGGGGAGAEETVVLDEAPPAVKRGVGVARGER 161
           F R+IW+   S+ GG A AEETVV DEAPP  +RGV     ER
Sbjct: 121 FSRVIWMEEHSIYGGDAMAEETVVFDEAPPP-RRGVEKVAVER 162

[Close]

BLAST of CSPI07G01850 vs. NCBI nr
Match: KAG6603237.1 (E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 128.3 bits (321), Expect = 6.2e-26
Identity = 74/164 (45.12%), Postives = 103/164 (62.80%), Query Frame = 0


Query: 1   MSFAISNPIQYRLY--ASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHN 60
           MSFA S PIQYRLY  A+ +  +  L+V +    ++IS S  TG P ++L +T   P ++
Sbjct: 1   MSFAASTPIQYRLYTAAAQSETHKALEVRMRSFNRLISASSFTGVPPQILFETRPTPVND 60

Query: 61  ALFYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEV 120
           ALF+LSLRQL  PLF+I ++LS+L+I  AA + +  +IAS I Q+   N ET+FHI+ EV
Sbjct: 61  ALFHLSLRQLHDPLFHIPRLLSTLDIHAAACEVIGWKIASQITQLSAGNRETSFHIVVEV 120

Query: 121 DFIRLIWL-------PSMGGGGAGAEETVVLDEAPPAVKRGVGV 156
           DFI+ IW+          G GG   EET+VLDE P   ++  GV
Sbjct: 121 DFIQNIWMGQVAGMHQGQGNGGGLVEETLVLDELPARRRQRRGV 164

The following BLAST results are available for this feature:

BLAST of CSPI07G01850 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI07G01850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K696	1.6e-83	100.00	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G026750 P...	[more]
A0A0A0K2W2	1.6e-83	100.00	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G025210 P...	[more]
A0A5D3E1S5	4.5e-67	80.75	E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S4DUZ4	4.5e-67	80.75	E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 ...	[more]
A0A5A7TLM1	3.9e-26	49.40	E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1...	[more]

BLAST of CSPI07G01850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144954.1	3.2e-83	100.00	uncharacterized protein LOC101214496 [Cucumis sativus] >KGN43349.1 hypothetical ...	[more]
XP_011659694.1	3.2e-83	100.00	E3 ubiquitin-protein ligase RDUF1 [Cucumis sativus] >KGN43356.1 hypothetical pro...	[more]
XP_016899530.1	9.4e-67	80.75	PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 ...	[more]
XP_038883574.1	9.1e-46	63.80	E3 ubiquitin-protein ligase RNF181-like [Benincasa hispida]	[more]
KAG6603237.1	6.2e-26	45.12	E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma ...	[more]

BLAST of CSPI07G01850 vs. NCBI nr


Match Name	E-value	Identity	Description

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G01850.1	CSPI07G01850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005634	nucleus
molecular_function	GO:0061630	ubiquitin protein ligase activity