CSPI07G00810 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G00810
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionEukaryotic translation initiation factor 4B3
LocationChr7: 785958 .. 787766 (+)
RNA-Seq ExpressionCSPI07G00810
SyntenyCSPI07G00810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAAATGGGAGAATCTCATCATTCACCGTTGATCCCCACATTGGACATTCCTCAACCGTGCGATGAGAACCTGTCATTCACCCATTTTATAACATTTGAGCCTCAACTCCGGCCATCCATTTTGCCGCAAACAAACGCAAACCTCAATTCTCACACAATCTCAAACCACAGCATCAATGGCGGCAATGGTTTCTTCTCCATGGGGAAAACCCGGCGCTTGGGCCCTCGATTCTGAGGAACACGATGCTCAACTCCTTAAAGAACAGGAACAGCAGAAACAACCTGAACTCAGCCTCGATTTCCCCTCCCTCAACGACGCCTTAGCCACCAAGCAGAAGAAGAAAGCCCAAACTCTCTCATTAGCCGAGTTCAACACCTATGCAACTACCGGTTCGAGATCTGAGGATTTTATTGTTCTACCCACCGGCCCTCGCCAACGGACGGCGGAGGAGCTCGACCGGAACAGGCTCGGTGGTGGGTTCAGGAATTATGGTTCGAATGGTTCGTATGAAAGGAATAGTAGGTATTCTAACGCGGATGATTCGTCTAATTCGAAGTGGGGTTCTTCTAGGGTTTCTGATGAGAATCGGAGGAGTAGTAATAGAGCGGATTCAAGTAAGGAATTTACGTCTTCTAGAGCTGACGAGGTTGATAATTGGGCTTTAACTAAGAAGCCCATGGCTGGAAATGGATTCGAGAGAAGGGAGAGAGGATTAGGGTTTTCGGATTCGCAATCGTCGAAAGCGGATGAATCAGAGAGTTGGGTTTCTAATAAGAGCAATATTCCGTCCGATGGCAGGAGATTTGACAGAGAGCGACGAGGAGGGTTCACAGCGAGCGGCGGCGGGGCAGATTCAGATAATTGGGGGAGGAAGAAAGAGGAAGGTAATACTGGTGGCGTCATTAGTAGTCGGCCAAAGCTTAGTCTACAGCCACGTACGTTACCGGTTGTCGATGGGAAATCGTCGGAGATATCAGCGACAGCAGCGAGGTCCAAGAGTTCCAACCCATTTGGAGCGGCGAGGCCGAGGGAAGAGGTACTAGCCGAGAAGGGACATGACTGGAAAAAGCTTGACGAGCAGCTAGAGTCGAAGATTAAGGAGGTGTCAGTGGAGGAGAAGACAGAAAAATCTAACCCCATTTTCTTTAGGAAAAGGAGCTTTGGCAATGGAAATAGCCGGGCTGCTGAGGACAGAACAGAGAGGAGCTGGAGAAAACCGGAGTCGCCACCGCCACAGGAGTATCCGGAATCTCGTCCCGAAAGGTTTGTTTAATGATCAACCGAAATTTGAATAGATAATTTCTATATTTCTTTCCTTGTTGTAGCTTTATCATCACATTAGCTAACTACCCATTTCTGTGTATTGATCGAATGGTGAAGCCTCATATATATCTGATATAATCTCAACTGGTAATTGCTTTTACAGTGGAGAGGAAAATGTAAATGGAGATTGCGAAGGCGAACCCGATCCCGAACGCGACGGGGACGAGAATGGAGCTTGACGGATGTGATGTGAAACTGAGAGATTGAAGTTTTGGGGTCGATGACAACATTCTCTAATGTTGTCATCTTTATAATTTTGCCGATGAGAAACAATTTTGATGTGTTCACTATAATGTTTGGAGCCAAACAACTACTGTGTTAACATTATTGTTCTAATCTTAAGTCCCATTTCCTCTGCAGAGTTATTTATTATTGATAATTGTAGTGTTTGTTTCTGGTTGGATTGCTTTGCTTGGAAACTGGATTTCGAACTGGATTTCGTGACACCTTACTTGAACAGGATCAGGATCGGTTCTACAGGCCA

mRNA sequence

AGAAAATGGGAGAATCTCATCATTCACCGTTGATCCCCACATTGGACATTCCTCAACCGTGCGATGAGAACCTGTCATTCACCCATTTTATAACATTTGAGCCTCAACTCCGGCCATCCATTTTGCCGCAAACAAACGCAAACCTCAATTCTCACACAATCTCAAACCACAGCATCAATGGCGGCAATGGTTTCTTCTCCATGGGGAAAACCCGGCGCTTGGGCCCTCGATTCTGAGGAACACGATGCTCAACTCCTTAAAGAACAGGAACAGCAGAAACAACCTGAACTCAGCCTCGATTTCCCCTCCCTCAACGACGCCTTAGCCACCAAGCAGAAGAAGAAAGCCCAAACTCTCTCATTAGCCGAGTTCAACACCTATGCAACTACCGGTTCGAGATCTGAGGATTTTATTGTTCTACCCACCGGCCCTCGCCAACGGACGGCGGAGGAGCTCGACCGGAACAGGCTCGGTGGTGGGTTCAGGAATTATGGTTCGAATGGTTCGTATGAAAGGAATAGTAGGTATTCTAACGCGGATGATTCGTCTAATTCGAAGTGGGGTTCTTCTAGGGTTTCTGATGAGAATCGGAGGAGTAGTAATAGAGCGGATTCAAGTAAGGAATTTACGTCTTCTAGAGCTGACGAGGTTGATAATTGGGCTTTAACTAAGAAGCCCATGGCTGGAAATGGATTCGAGAGAAGGGAGAGAGGATTAGGGTTTTCGGATTCGCAATCGTCGAAAGCGGATGAATCAGAGAGTTGGGTTTCTAATAAGAGCAATATTCCGTCCGATGGCAGGAGATTTGACAGAGAGCGACGAGGAGGGTTCACAGCGAGCGGCGGCGGGGCAGATTCAGATAATTGGGGGAGGAAGAAAGAGGAAGGTAATACTGGTGGCGTCATTAGTAGTCGGCCAAAGCTTAGTCTACAGCCACGTACGTTACCGGTTGTCGATGGGAAATCGTCGGAGATATCAGCGACAGCAGCGAGGTCCAAGAGTTCCAACCCATTTGGAGCGGCGAGGCCGAGGGAAGAGGTACTAGCCGAGAAGGGACATGACTGGAAAAAGCTTGACGAGCAGCTAGAGTCGAAGATTAAGGAGGTGTCAGTGGAGGAGAAGACAGAAAAATCTAACCCCATTTTCTTTAGGAAAAGGAGCTTTGGCAATGGAAATAGCCGGGCTGCTGAGGACAGAACAGAGAGGAGCTGGAGAAAACCGGAGTCGCCACCGCCACAGGAGTATCCGGAATCTCGTCCCGAAAGTGGAGAGGAAAATGTAAATGGAGATTGCGAAGGCGAACCCGATCCCGAACGCGACGGGGACGAGAATGGAGCTTGACGGATGTGATGTGAAACTGAGAGATTGAAGTTTTGGGGTCGATGACAACATTCTCTAATGTTGTCATCTTTATAATTTTGCCGATGAGAAACAATTTTGATGTGTTCACTATAATGTTTGGAGCCAAACAACTACTGTGTTAACATTATTGTTCTAATCTTAAGTCCCATTTCCTCTGCAGAGTTATTTATTATTGATAATTGTAGTGTTTGTTTCTGGTTGGATTGCTTTGCTTGGAAACTGGATTTCGAACTGGATTTCGTGACACCTTACTTGAACAGGATCAGGATCGGTTCTACAGGCCA

Coding sequence (CDS)

ATGGCGGCAATGGTTTCTTCTCCATGGGGAAAACCCGGCGCTTGGGCCCTCGATTCTGAGGAACACGATGCTCAACTCCTTAAAGAACAGGAACAGCAGAAACAACCTGAACTCAGCCTCGATTTCCCCTCCCTCAACGACGCCTTAGCCACCAAGCAGAAGAAGAAAGCCCAAACTCTCTCATTAGCCGAGTTCAACACCTATGCAACTACCGGTTCGAGATCTGAGGATTTTATTGTTCTACCCACCGGCCCTCGCCAACGGACGGCGGAGGAGCTCGACCGGAACAGGCTCGGTGGTGGGTTCAGGAATTATGGTTCGAATGGTTCGTATGAAAGGAATAGTAGGTATTCTAACGCGGATGATTCGTCTAATTCGAAGTGGGGTTCTTCTAGGGTTTCTGATGAGAATCGGAGGAGTAGTAATAGAGCGGATTCAAGTAAGGAATTTACGTCTTCTAGAGCTGACGAGGTTGATAATTGGGCTTTAACTAAGAAGCCCATGGCTGGAAATGGATTCGAGAGAAGGGAGAGAGGATTAGGGTTTTCGGATTCGCAATCGTCGAAAGCGGATGAATCAGAGAGTTGGGTTTCTAATAAGAGCAATATTCCGTCCGATGGCAGGAGATTTGACAGAGAGCGACGAGGAGGGTTCACAGCGAGCGGCGGCGGGGCAGATTCAGATAATTGGGGGAGGAAGAAAGAGGAAGGTAATACTGGTGGCGTCATTAGTAGTCGGCCAAAGCTTAGTCTACAGCCACGTACGTTACCGGTTGTCGATGGGAAATCGTCGGAGATATCAGCGACAGCAGCGAGGTCCAAGAGTTCCAACCCATTTGGAGCGGCGAGGCCGAGGGAAGAGGTACTAGCCGAGAAGGGACATGACTGGAAAAAGCTTGACGAGCAGCTAGAGTCGAAGATTAAGGAGGTGTCAGTGGAGGAGAAGACAGAAAAATCTAACCCCATTTTCTTTAGGAAAAGGAGCTTTGGCAATGGAAATAGCCGGGCTGCTGAGGACAGAACAGAGAGGAGCTGGAGAAAACCGGAGTCGCCACCGCCACAGGAGTATCCGGAATCTCGTCCCGAAAGTGGAGAGGAAAATGTAAATGGAGATTGCGAAGGCGAACCCGATCCCGAACGCGACGGGGACGAGAATGGAGCTTGA

Protein sequence

MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTLSLAEFNTYATTGSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSNADDSSNSKWGSSRVSDENRRSSNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRERGLGFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEGNTGGVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYPESRPESGEENVNGDCEGEPDPERDGDENGA*
Homology
BLAST of CSPI07G00810 vs. ExPASy Swiss-Prot
Match: Q9SZP8 (Eukaryotic translation initiation factor 4B3 OS=Arabidopsis thaliana OX=3702 GN=EIF4B3 PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.5e-73
Identity = 199/410 (48.54%), Postives = 252/410 (61.46%), Query Frame = 0

Query: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKE---QEQQKQPELSLDFPSLNDALATK-QKKK 60
           MAA VSS W KPGAWAL++EEH+A+L ++     Q+   E S DFPSL  A  TK +KKK
Sbjct: 1   MAAAVSSVWAKPGAWALEAEEHEAELKQQPSPTNQKSSAEDSSDFPSLAAAATTKTKKKK 60

Query: 61  AQTLSLAEFNTYATTGSR---------SEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGS 120
            QT+SLAEF TY T  ++           + + LPTGPR+R+AEELDR++LGGGFR+YG 
Sbjct: 61  GQTISLAEFATYGTAKAKPAPQTERLTQAELVALPTGPRERSAEELDRSKLGGGFRSYG- 120

Query: 121 NGSYERNSRYSNADDSSNSKWGSSRVSDENRRSS---NR-ADSSKEFTSSRADEVDNWAL 180
                   RY   D+SS+S+WGSSRVS++  R     NR  + S++   SRADE DNWA 
Sbjct: 121 ------GGRY--GDESSSSRWGSSRVSEDGERRGGGFNRDREPSRDSGPSRADEDDNWAA 180

Query: 181 TKKPMAGNGFERRERGL--GFSDSQS-SKADESESWVSNKSNIPSDGRRF--------DR 240
            KKP++GNGFERRERG   GF +SQS SKADE +SWVS K   PS+ RRF        DR
Sbjct: 181 AKKPISGNGFERRERGSGGGFFESQSQSKADEVDSWVSTK---PSEPRRFVSSNGGGGDR 240

Query: 241 -ERRGGFTA----------SGGGADSDNWGRKKEE-----GNTGGVISSRPKLSLQPRTL 300
            E+RG F +           GGG++SD WGR++EE     G+      SRP+L LQPRTL
Sbjct: 241 FEKRGSFESLSRNRDSQYGGGGGSESDTWGRRREESGAANGSPPPSGGSRPRLVLQPRTL 300

Query: 301 PV----VDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKLDEQLES-KIKEVS 360
           PV    V    S +     + K +NPFG ARPREEVLAEKG DWK++DE+LE+ K+K+++
Sbjct: 301 PVAVVEVVKPESPVLVIVEKPKGANPFGNARPREEVLAEKGQDWKEIDEKLEAEKLKDIA 360

Query: 361 VEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYPESRP 362
                EK N     K  FG GN R  E+R ERSWRK      ++  E  P
Sbjct: 361 A--AMEKPNEKSTGKMGFGLGNGRKDEERIERSWRKSTEHSEEDAQEEEP 396

BLAST of CSPI07G00810 vs. ExPASy Swiss-Prot
Match: Q9SAD7 (Eukaryotic translation initiation factor 4B2 OS=Arabidopsis thaliana OX=3702 GN=EIF4B2 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.5e-28
Identity = 121/356 (33.99%), Postives = 164/356 (46.07%), Query Frame = 0

Query: 5   VSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTLSLAE 64
           +S PWG  GAWA ++E  D +  +  E       S  FPSL +A   K  KK + ++L+E
Sbjct: 1   MSKPWGGIGAWADEAERADEE--QAAEATATAADSQSFPSLKEAATAKSSKKKKKMTLSE 60

Query: 65  FNTYATT-----GSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSN 124
           F   A T     G   E  + LPTGPRQR+ +E+   RLGGGF +YG   S     R S 
Sbjct: 61  FTKGAYTAPSSAGLTREQMLQLPTGPRQRSEDEMQPGRLGGGFSSYGGGRSSGPPGRMSR 120

Query: 125 ADDSSNSKWGS--------SRVSDENRRSSNRADSSKEFTSSRADEVDNWALTKKPM--- 184
             + S+  WG             D+ R S++R     +   SRADE D+W   KK +   
Sbjct: 121 DREDSDGSWGGGGGGRRSYGGFDDDQRGSNSRVSDFPQV--SRADEDDDWGKGKKSLPSF 180

Query: 185 -----------------AGNGFERRERGLGFSDSQS------SKADESESWVSNKSNIPS 244
                             G G      G G + + S      SKADE ++W + K+   +
Sbjct: 181 DQGRQGSRYGGGGGSFGGGGGGGAGSYGGGGAGAGSGGGGGFSKADEVDNWAAGKAKSST 240

Query: 245 DGRRFDRERRGGFTASGGGADSDNWGRKKEEGNTGGVISSRPKLSLQPRTLPVVDGKSSE 304
            G         GF  S  G + D W R       GGV   R +L  +PR     D + SE
Sbjct: 241 FG--------SGFRES--GPEPDRWARGVLPSG-GGVQEERRRLVFEPRK---ADTEVSE 300

Query: 305 ISATAARSKSSNPFGAARPREEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSNP 322
            + TA ++   +PFGAARPRE+VLAEKG DWKKLD  +E+K  + S     + S P
Sbjct: 301 -TPTAVKTSKPSPFGAARPREQVLAEKGLDWKKLDSDIEAKKGQTSRPSSAQSSRP 337

BLAST of CSPI07G00810 vs. ExPASy Swiss-Prot
Match: Q9AUJ7 (Eukaryotic translation initiation factor 4B1 OS=Triticum aestivum OX=4565 GN=EIF4B PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 4.8e-27
Identity = 122/379 (32.19%), Postives = 177/379 (46.70%), Query Frame = 0

Query: 5   VSSPWGKPGAWALDSEEHD-----AQLLKEQEQQKQPELSLDFPSLNDALAT----KQKK 64
           ++ PWG  GAWALD+E  D     A      +       +  FPSL +A+      ++KK
Sbjct: 1   MAKPWGGVGAWALDAEREDEEREHAAAFPAPDPPAAAGGAASFPSLKEAVVAGGGKQKKK 60

Query: 65  KAQTLSLAEFNTYATTGS------------RSEDFIVLPTGPRQRTAEELDRNRLGGGFR 124
           K  TLSL+EF TY   G+              ++ ++LPTGPR+R+ +ELDR+R   GFR
Sbjct: 61  KGTTLSLSEFTTYGAAGAPRRVAPAEPKGLTPQEMMMLPTGPRERSEDELDRSR---GFR 120

Query: 125 NYGSN-----GSYE---RNSRYSN------ADDSSNSKWGSSRVSDENRRSSNRADS-SK 184
           +YG +     G ++   R+SR S+      AD+S N  WG ++        S R D  S 
Sbjct: 121 SYGGDREPRGGGFDDDRRSSRDSDLDMPSRADESDN--WGKNKSFSPAPTDSGRRDRLSG 180

Query: 185 EFTSSRADEVDNWALTKKPMAGNGFERRERGLGFSDSQSSKADESESWVSNKSNIPSDGR 244
                R+D++DNW+  KKP+    +     G GF +S      E            S G 
Sbjct: 181 PSPLGRSDDIDNWSRDKKPLPSR-YPSLGTGGGFRESSGGGFRE------------SSGG 240

Query: 245 RFDRERRGGFTASGGGADSDNWGRKKEEG---NTGGVISSRPKLSLQPRTLPVVDGKSSE 304
            F     GGF  S G +DSD W R        N G     RP+L+L P   P  D  ++ 
Sbjct: 241 GFRESSGGGFRDSPGPSDSDRWVRGAVPAPMTNNG----DRPRLNLNP---PKRDPSATP 300

Query: 305 ISATAARSKSSNPFGAARPREEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSNPIFFR 345
           + A        +PFGAA+PREEVLAEKG DW+K++ ++E K    +    +  ++    R
Sbjct: 301 VPAAEVARSRPSPFGAAKPREEVLAEKGLDWRKMEGEIEKKTSRPTSSHSSRPNSAHSSR 354

BLAST of CSPI07G00810 vs. ExPASy Swiss-Prot
Match: Q9LIN5 (Eukaryotic translation initiation factor 4B1 OS=Arabidopsis thaliana OX=3702 GN=EIF4B1 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 9.3e-23
Identity = 116/338 (34.32%), Postives = 157/338 (46.45%), Query Frame = 0

Query: 5   VSSPWG--KPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALA----TKQKKKAQ 64
           +S  WG    GAWA ++E  D +   E         S  FPSL +A A    + + +K +
Sbjct: 1   MSKAWGGIGIGAWADEAERADEEQAAEATAATADTQS--FPSLREAAAATATSGKSRKMK 60

Query: 65  TLSLAEFNTYATT--------GSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYG--SN 124
            +SL+EF T A T        G   ++ + LPTGPRQR+ EE+   RLGGGF +YG  S 
Sbjct: 61  KMSLSEFTTGAYTAPGGRNSVGLTQQEILQLPTGPRQRSEEEMQPGRLGGGFSSYGGRSG 120

Query: 125 GSYERNSRYSNADDSSNSKWGSSRV----SDENRRSSNRADSSKEFTSSRADEVDNWALT 184
           G   R+   S+   S     G  R      D++RR  N++  S     SRADEVD+W   
Sbjct: 121 GRIGRDRDDSDGSWSGGGGGGGRRPYGGGFDDDRR-GNQSRVSDFPQPSRADEVDDWGKE 180

Query: 185 KKPMAGNGFERRERGLGFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGG 244
           KKP+    F++  +G    D                      G  F     GG    GGG
Sbjct: 181 KKPLP--SFDQGRQGRYSGDG---------------GGFGGGGSGFG----GGGGGGGGG 240

Query: 245 A----DSDNWGRKKEE------------GNTGGVISSRPKLSLQPRTLPVVDGKSSEISA 304
                D DNWG  K +            G++G     R +L L+PR    V+   SE   
Sbjct: 241 LSRADDVDNWGAGKRQAPVRSSTFGSSFGDSGQ--EERRRLVLEPRK---VESGGSETPP 300

Query: 305 TAARSKSSNPFGAARPREEVLAEKGHDWKKLDEQLESK 307
              ++   NPFGAARPRE+VLAEKG DWKK+D ++E+K
Sbjct: 301 VVEKTSKPNPFGAARPREDVLAEKGLDWKKIDSEIEAK 309

BLAST of CSPI07G00810 vs. ExPASy TrEMBL
Match: A0A0A0K4M1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G009760 PE=4 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 1.4e-207
Identity = 385/387 (99.48%), Postives = 385/387 (99.48%), Query Frame = 0

Query: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTL 60
           MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTL
Sbjct: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTL 60

Query: 61  SLAEFNTYATTGSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSNA 120
           SLAEFNTYATTGSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSNA
Sbjct: 61  SLAEFNTYATTGSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSNA 120

Query: 121 DDSSNSKWGSSRVSDENRRSSNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRERGL 180
           DDSSNSKWGSSRVSDENRRSSNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRERGL
Sbjct: 121 DDSSNSKWGSSRVSDENRRSSNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRERGL 180

Query: 181 GFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEGNTG 240
           GFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEGN G
Sbjct: 181 GFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEGNAG 240

Query: 241 GVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKLD 300
           GVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKLD
Sbjct: 241 GVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKLD 300

Query: 301 EQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYPESR 360
           EQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYPESR
Sbjct: 301 EQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYPESR 360

Query: 361 PESGEENVNGDCEGEPDPERDGDENGA 388
           PESGEENVNGDCEGEPDPERDG ENGA
Sbjct: 361 PESGEENVNGDCEGEPDPERDGGENGA 387

BLAST of CSPI07G00810 vs. ExPASy TrEMBL
Match: A0A5A7U3A1 (Eukaryotic translation initiation factor 4B3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00800 PE=4 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 1.8e-194
Identity = 369/390 (94.62%), Postives = 374/390 (95.90%), Query Frame = 0

Query: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTL 60
           MAAMVSSPWGKPGAWALDSEEH+AQLLKEQEQQKQPELSLDFPSLNDALATKQKKK QTL
Sbjct: 1   MAAMVSSPWGKPGAWALDSEEHEAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKGQTL 60

Query: 61  SLAEFNTYATTGSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSNA 120
           SLAEFNTY TTGSRSEDF+VLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERN+RYSNA
Sbjct: 61  SLAEFNTYGTTGSRSEDFMVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNNRYSNA 120

Query: 121 DDSSNSKWGSSRVSDENRRS---SNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRE 180
           DDSSNSKWGSSRVSDENRRS   SNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRE
Sbjct: 121 DDSSNSKWGSSRVSDENRRSSNGSNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRE 180

Query: 181 RGLGFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEG 240
           RGLGFSDSQSSKADESESWVSNKSNIPS GRRFDRERRGGFTASGGGADSDNWGRKKEEG
Sbjct: 181 RGLGFSDSQSSKADESESWVSNKSNIPSGGRRFDRERRGGFTASGGGADSDNWGRKKEEG 240

Query: 241 NTGGVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWK 300
           N GGVI SRPKLSLQPRTLPV DGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWK
Sbjct: 241 NAGGVIGSRPKLSLQPRTLPVGDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWK 300

Query: 301 KLDEQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYP 360
           KLDEQLESKIKEVSVEEKTEKSN I FRKRSFGNGNSRAAEDRTE+SWRKPESPPPQE P
Sbjct: 301 KLDEQLESKIKEVSVEEKTEKSNAISFRKRSFGNGNSRAAEDRTEKSWRKPESPPPQESP 360

Query: 361 ESRPESGEENVNGDCEGEPDPERDGDENGA 388
           ESRPESGEENVNGDCEG+   E DGDENGA
Sbjct: 361 ESRPESGEENVNGDCEGK--HEHDGDENGA 388

BLAST of CSPI07G00810 vs. ExPASy TrEMBL
Match: A0A1S3BJA3 (eukaryotic translation initiation factor 4B3 OS=Cucumis melo OX=3656 GN=LOC103490303 PE=4 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 3.8e-192
Identity = 366/390 (93.85%), Postives = 371/390 (95.13%), Query Frame = 0

Query: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTL 60
           MAAMVSSPWGKPGAWALDSEEH+AQLLKEQEQQKQPELSLDFPSLNDALATKQKKK QTL
Sbjct: 1   MAAMVSSPWGKPGAWALDSEEHEAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKGQTL 60

Query: 61  SLAEFNTYATTGSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSNA 120
           SLAEFNTY TT SRSEDF+ LPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERN+RYSNA
Sbjct: 61  SLAEFNTYGTTDSRSEDFMELPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNNRYSNA 120

Query: 121 DDSSNSKWGSSRVSDENRRS---SNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRE 180
           DDSSNSKWGSSRVSDENRRS   SNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRE
Sbjct: 121 DDSSNSKWGSSRVSDENRRSSNGSNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRE 180

Query: 181 RGLGFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEG 240
           RGLGFSDSQSSKADESESWVSNKSNIPS GRRFDRERRGGFTASGGGADSDNWGRKKEEG
Sbjct: 181 RGLGFSDSQSSKADESESWVSNKSNIPSGGRRFDRERRGGFTASGGGADSDNWGRKKEEG 240

Query: 241 NTGGVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWK 300
           N GGVI SRPKLSLQPRTLPV DGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWK
Sbjct: 241 NAGGVIGSRPKLSLQPRTLPVGDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWK 300

Query: 301 KLDEQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYP 360
           KLDEQLESKIKEVSVEEKTEKSN I FRKRSF NGNSRAAEDRTE+SWRKPESPPPQE P
Sbjct: 301 KLDEQLESKIKEVSVEEKTEKSNAISFRKRSFDNGNSRAAEDRTEKSWRKPESPPPQESP 360

Query: 361 ESRPESGEENVNGDCEGEPDPERDGDENGA 388
           ESRPESGEENVNGDCEG+   E DGDENGA
Sbjct: 361 ESRPESGEENVNGDCEGK--REHDGDENGA 388

BLAST of CSPI07G00810 vs. ExPASy TrEMBL
Match: A0A6J1ERF5 (eukaryotic translation initiation factor 4B3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435193 PE=4 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 6.7e-149
Identity = 309/408 (75.74%), Postives = 338/408 (82.84%), Query Frame = 0

Query: 5   VSSPWGKPGAWALDSEEHDAQLLKE-------QEQQKQPELSLDFPSLNDALATKQKKKA 64
           ++SPWGKPGAWALDSEEH+A+LLKE       Q+QQK+ ELSLDFPSLNDALATKQKKK 
Sbjct: 4   MASPWGKPGAWALDSEEHEAELLKEEQEQQQQQQQQKKAELSLDFPSLNDALATKQKKKG 63

Query: 65  QTLSLAEFNTYATTGSR-------SEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGS 124
           QTLSLAEF  +A +GS+        ED +VLPTGPR+RTAEELDRN+LGGGFRNYGSNGS
Sbjct: 64  QTLSLAEFTAHAGSGSKYSSQGRTHEDVLVLPTGPRERTAEELDRNKLGGGFRNYGSNGS 123

Query: 125 YERNSRYSNADDSSNSKWGSSRVSDENRRSS---NRADSSKEFTSSRADEVDNWALTKKP 184
           YER++RYSN DDSSNSKWGSSRVSDENRR+    NR DSSKEF  SRADEVDNWA TKKP
Sbjct: 124 YERSNRYSNGDDSSNSKWGSSRVSDENRRNDGGFNRGDSSKEFVPSRADEVDNWASTKKP 183

Query: 185 MAGNGFERRERGLGFSDSQSSKADESESWVSNKSNIPSDGRR------FDRERRGGFTAS 244
           MAGNGFERR+RGLGFSDSQSSKADESESWVSNKSN+PSD RR      FDRERRGGF AS
Sbjct: 184 MAGNGFERRDRGLGFSDSQSSKADESESWVSNKSNVPSDARRFGANGGFDRERRGGFPAS 243

Query: 245 GGGADSDNWGRKKEEGNT--GGVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPF 304
           GGGADSD WGRKK+EGN+  GG   SRPKL+LQPRTLPV D KSSEIS T ARSKSSNPF
Sbjct: 244 GGGADSDTWGRKKDEGNSGGGGGGGSRPKLNLQPRTLPVNDVKSSEISGTTARSKSSNPF 303

Query: 305 GAARPREEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAED 364
           G ARPREEVLAEKGHDWKKLDE+LE KIKEVS+ EKTEKSN I   K+SFGNG +RAAED
Sbjct: 304 GEARPREEVLAEKGHDWKKLDEELELKIKEVSM-EKTEKSNAI--GKKSFGNGTTRAAED 363

Query: 365 RTERSWRKPESPPPQEYPESRPESGEENVNGDCEGEPDPERDGDENGA 388
           RTERSWRKPES PP+E PESRP+S E+N NG        E DG++N A
Sbjct: 364 RTERSWRKPES-PPEESPESRPDSVEDNENG--------EEDGNKNEA 399

BLAST of CSPI07G00810 vs. ExPASy TrEMBL
Match: A0A6J1EKJ0 (eukaryotic translation initiation factor 4B3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435193 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 4.1e-146
Identity = 309/430 (71.86%), Postives = 337/430 (78.37%), Query Frame = 0

Query: 5   VSSPWGKPGAWALDSEEHDAQLLKE-------QEQQKQPELSLDFPSLNDALATKQKKKA 64
           ++SPWGKPGAWALDSEEH+A+LLKE       Q+QQK+ ELSLDFPSLNDALATKQKKK 
Sbjct: 4   MASPWGKPGAWALDSEEHEAELLKEEQEQQQQQQQQKKAELSLDFPSLNDALATKQKKKG 63

Query: 65  QTLSLAEFNTYATTGSR-------SEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGS 124
           QTLSLAEF  +A +GS+        ED +VLPTGPR+RTAEELDRN+LGGGFRNYGSNGS
Sbjct: 64  QTLSLAEFTAHAGSGSKYSSQGRTHEDVLVLPTGPRERTAEELDRNKLGGGFRNYGSNGS 123

Query: 125 YERNSRYSNADDSSNSKWGSSRVSDENRRSS---NRADSSKEFTSSRADEVDNWALTKKP 184
           YER++RYSN DDSSNSKWGSSRVSDENRR+    NR DSSKEF  SRADEVDNWA TKKP
Sbjct: 124 YERSNRYSNGDDSSNSKWGSSRVSDENRRNDGGFNRGDSSKEFVPSRADEVDNWASTKKP 183

Query: 185 MAGNGFERRERGLGFSDSQSSKADESESWVSNKSNIPSDGRR------FDRERRGGFTAS 244
           MAGNGFERR+RGLGFSDSQSSKADESESWVSNKSN+PSD RR      FDRERRGGF AS
Sbjct: 184 MAGNGFERRDRGLGFSDSQSSKADESESWVSNKSNVPSDARRFGANGGFDRERRGGFPAS 243

Query: 245 GGGADSDNWGRKKEEGNT------------------------GGVISSRPKLSLQPRTLP 304
           GGGADSD WGRKK+EGN                         GG   SRPKL+LQPRTLP
Sbjct: 244 GGGADSDTWGRKKDEGNIGGGGGGGADSDTWGRKKDEGNSGGGGGGGSRPKLNLQPRTLP 303

Query: 305 VVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKLDEQLESKIKEVSVEEKTE 364
           V D KSSEIS T ARSKSSNPFG ARPREEVLAEKGHDWKKLDE+LE KIKEVS+ EKTE
Sbjct: 304 VNDVKSSEISGTTARSKSSNPFGEARPREEVLAEKGHDWKKLDEELELKIKEVSM-EKTE 363

Query: 365 KSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYPESRPESGEENVNGDCEGEPD 388
           KSN I   K+SFGNG +RAAEDRTERSWRKPES PP+E PESRP+S E+N NG       
Sbjct: 364 KSNAI--GKKSFGNGTTRAAEDRTERSWRKPES-PPEESPESRPDSVEDNENG------- 421

BLAST of CSPI07G00810 vs. NCBI nr
Match: XP_004144838.1 (eukaryotic translation initiation factor 4B3 [Cucumis sativus] >KGN43232.1 hypothetical protein Csa_020473 [Cucumis sativus])

HSP 1 Score: 731.9 bits (1888), Expect = 2.9e-207
Identity = 385/387 (99.48%), Postives = 385/387 (99.48%), Query Frame = 0

Query: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTL 60
           MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTL
Sbjct: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTL 60

Query: 61  SLAEFNTYATTGSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSNA 120
           SLAEFNTYATTGSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSNA
Sbjct: 61  SLAEFNTYATTGSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSNA 120

Query: 121 DDSSNSKWGSSRVSDENRRSSNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRERGL 180
           DDSSNSKWGSSRVSDENRRSSNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRERGL
Sbjct: 121 DDSSNSKWGSSRVSDENRRSSNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRERGL 180

Query: 181 GFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEGNTG 240
           GFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEGN G
Sbjct: 181 GFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEGNAG 240

Query: 241 GVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKLD 300
           GVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKLD
Sbjct: 241 GVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKLD 300

Query: 301 EQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYPESR 360
           EQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYPESR
Sbjct: 301 EQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYPESR 360

Query: 361 PESGEENVNGDCEGEPDPERDGDENGA 388
           PESGEENVNGDCEGEPDPERDG ENGA
Sbjct: 361 PESGEENVNGDCEGEPDPERDGGENGA 387

BLAST of CSPI07G00810 vs. NCBI nr
Match: KAA0049734.1 (eukaryotic translation initiation factor 4B3 [Cucumis melo var. makuwa] >TYK12143.1 eukaryotic translation initiation factor 4B3 [Cucumis melo var. makuwa])

HSP 1 Score: 688.3 bits (1775), Expect = 3.7e-194
Identity = 369/390 (94.62%), Postives = 374/390 (95.90%), Query Frame = 0

Query: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTL 60
           MAAMVSSPWGKPGAWALDSEEH+AQLLKEQEQQKQPELSLDFPSLNDALATKQKKK QTL
Sbjct: 1   MAAMVSSPWGKPGAWALDSEEHEAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKGQTL 60

Query: 61  SLAEFNTYATTGSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSNA 120
           SLAEFNTY TTGSRSEDF+VLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERN+RYSNA
Sbjct: 61  SLAEFNTYGTTGSRSEDFMVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNNRYSNA 120

Query: 121 DDSSNSKWGSSRVSDENRRS---SNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRE 180
           DDSSNSKWGSSRVSDENRRS   SNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRE
Sbjct: 121 DDSSNSKWGSSRVSDENRRSSNGSNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRE 180

Query: 181 RGLGFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEG 240
           RGLGFSDSQSSKADESESWVSNKSNIPS GRRFDRERRGGFTASGGGADSDNWGRKKEEG
Sbjct: 181 RGLGFSDSQSSKADESESWVSNKSNIPSGGRRFDRERRGGFTASGGGADSDNWGRKKEEG 240

Query: 241 NTGGVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWK 300
           N GGVI SRPKLSLQPRTLPV DGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWK
Sbjct: 241 NAGGVIGSRPKLSLQPRTLPVGDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWK 300

Query: 301 KLDEQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYP 360
           KLDEQLESKIKEVSVEEKTEKSN I FRKRSFGNGNSRAAEDRTE+SWRKPESPPPQE P
Sbjct: 301 KLDEQLESKIKEVSVEEKTEKSNAISFRKRSFGNGNSRAAEDRTEKSWRKPESPPPQESP 360

Query: 361 ESRPESGEENVNGDCEGEPDPERDGDENGA 388
           ESRPESGEENVNGDCEG+   E DGDENGA
Sbjct: 361 ESRPESGEENVNGDCEGK--HEHDGDENGA 388

BLAST of CSPI07G00810 vs. NCBI nr
Match: XP_008447975.1 (PREDICTED: eukaryotic translation initiation factor 4B3 [Cucumis melo])

HSP 1 Score: 680.6 bits (1755), Expect = 7.8e-192
Identity = 366/390 (93.85%), Postives = 371/390 (95.13%), Query Frame = 0

Query: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTL 60
           MAAMVSSPWGKPGAWALDSEEH+AQLLKEQEQQKQPELSLDFPSLNDALATKQKKK QTL
Sbjct: 1   MAAMVSSPWGKPGAWALDSEEHEAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKGQTL 60

Query: 61  SLAEFNTYATTGSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSNA 120
           SLAEFNTY TT SRSEDF+ LPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERN+RYSNA
Sbjct: 61  SLAEFNTYGTTDSRSEDFMELPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNNRYSNA 120

Query: 121 DDSSNSKWGSSRVSDENRRS---SNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRE 180
           DDSSNSKWGSSRVSDENRRS   SNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRE
Sbjct: 121 DDSSNSKWGSSRVSDENRRSSNGSNRADSSKEFTSSRADEVDNWALTKKPMAGNGFERRE 180

Query: 181 RGLGFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGGADSDNWGRKKEEG 240
           RGLGFSDSQSSKADESESWVSNKSNIPS GRRFDRERRGGFTASGGGADSDNWGRKKEEG
Sbjct: 181 RGLGFSDSQSSKADESESWVSNKSNIPSGGRRFDRERRGGFTASGGGADSDNWGRKKEEG 240

Query: 241 NTGGVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWK 300
           N GGVI SRPKLSLQPRTLPV DGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWK
Sbjct: 241 NAGGVIGSRPKLSLQPRTLPVGDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWK 300

Query: 301 KLDEQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYP 360
           KLDEQLESKIKEVSVEEKTEKSN I FRKRSF NGNSRAAEDRTE+SWRKPESPPPQE P
Sbjct: 301 KLDEQLESKIKEVSVEEKTEKSNAISFRKRSFDNGNSRAAEDRTEKSWRKPESPPPQESP 360

Query: 361 ESRPESGEENVNGDCEGEPDPERDGDENGA 388
           ESRPESGEENVNGDCEG+   E DGDENGA
Sbjct: 361 ESRPESGEENVNGDCEGK--REHDGDENGA 388

BLAST of CSPI07G00810 vs. NCBI nr
Match: XP_038887904.1 (eukaryotic translation initiation factor 4B3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 625.9 bits (1613), Expect = 2.3e-175
Identity = 349/403 (86.60%), Postives = 361/403 (89.58%), Query Frame = 0

Query: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTL 60
           MAAMV SPWGKPGAWALDSEEH+AQLLKEQE QKQ ELSLDFPSLNDALATKQKKK QTL
Sbjct: 1   MAAMV-SPWGKPGAWALDSEEHEAQLLKEQELQKQAELSLDFPSLNDALATKQKKKGQTL 60

Query: 61  SLAEFNTYATTGSRS-------EDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYER 120
           SLAEFNTY +TGSRS       ED +VLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYER
Sbjct: 61  SLAEFNTYGSTGSRSSSQSLRHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYER 120

Query: 121 NSRYSNADDSSNSKWGSSRVSDENRRS---SNRADSSKEFTSSRADEVDNWALTKKPMAG 180
           N+RYSN DDSSNSKWGSSRVSDENRR+   SNRADSSKEFTSSRADEVDNWALTKKPMAG
Sbjct: 121 NNRYSNGDDSSNSKWGSSRVSDENRRNGNGSNRADSSKEFTSSRADEVDNWALTKKPMAG 180

Query: 181 NGFERRERGLGFSDSQSSKADESESWVSNKSNIPSDGRR------FDRERRGGFTASGGG 240
           NGFERRERG+GFSDSQSSKADESESWVSNK+N+PSDGRR      FDRERRGGFTASGGG
Sbjct: 181 NGFERRERGVGFSDSQSSKADESESWVSNKTNVPSDGRRFGANGGFDRERRGGFTASGGG 240

Query: 241 ADSDNWGRKKEEGNTGGVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARP 300
           ADSDNWGRKKEEGNTGGVI SRPKL+LQPRTLPV DGKSSEIS TAARSKSSNPFGAARP
Sbjct: 241 ADSDNWGRKKEEGNTGGVIGSRPKLNLQPRTLPVSDGKSSEISGTAARSKSSNPFGAARP 300

Query: 301 REEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERS 360
           REEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSN I F K+SFGNG SRA EDRTE+S
Sbjct: 301 REEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSNAISFGKKSFGNGKSRAGEDRTEKS 360

Query: 361 WRKPESPPPQEYPESRPESGEENVNGDCEGEPDPERDGDENGA 388
           WRKPES P QE  ESRP+SGEEN   DCEG    ERDGDENGA
Sbjct: 361 WRKPES-PLQESLESRPDSGEENEIRDCEG----ERDGDENGA 397

BLAST of CSPI07G00810 vs. NCBI nr
Match: XP_038887905.1 (eukaryotic translation initiation factor 4B3-like isoform X2 [Benincasa hispida])

HSP 1 Score: 602.1 bits (1551), Expect = 3.5e-168
Identity = 332/379 (87.60%), Postives = 344/379 (90.77%), Query Frame = 0

Query: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTL 60
           MAAMV SPWGKPGAWALDSEEH+AQLLKEQE QKQ ELSLDFPSLNDALATKQKKK QTL
Sbjct: 1   MAAMV-SPWGKPGAWALDSEEHEAQLLKEQELQKQAELSLDFPSLNDALATKQKKKGQTL 60

Query: 61  SLAEFNTYATTGSRS-------EDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYER 120
           SLAEFNTY +TGSRS       ED +VLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYER
Sbjct: 61  SLAEFNTYGSTGSRSSSQSLRHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYER 120

Query: 121 NSRYSNADDSSNSKWGSSRVSDENRRS---SNRADSSKEFTSSRADEVDNWALTKKPMAG 180
           N+RYSN DDSSNSKWGSSRVSDENRR+   SNRADSSKEFTSSRADEVDNWALTKKPMAG
Sbjct: 121 NNRYSNGDDSSNSKWGSSRVSDENRRNGNGSNRADSSKEFTSSRADEVDNWALTKKPMAG 180

Query: 181 NGFERRERGLGFSDSQSSKADESESWVSNKSNIPSDGRR------FDRERRGGFTASGGG 240
           NGFERRERG+GFSDSQSSKADESESWVSNK+N+PSDGRR      FDRERRGGFTASGGG
Sbjct: 181 NGFERRERGVGFSDSQSSKADESESWVSNKTNVPSDGRRFGANGGFDRERRGGFTASGGG 240

Query: 241 ADSDNWGRKKEEGNTGGVISSRPKLSLQPRTLPVVDGKSSEISATAARSKSSNPFGAARP 300
           ADSDNWGRKKEEGNTGGVI SRPKL+LQPRTLPV DGKSSEIS TAARSKSSNPFGAARP
Sbjct: 241 ADSDNWGRKKEEGNTGGVIGSRPKLNLQPRTLPVSDGKSSEISGTAARSKSSNPFGAARP 300

Query: 301 REEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERS 360
           REEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSN I F K+SFGNG SRA EDRTE+S
Sbjct: 301 REEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSNAISFGKKSFGNGKSRAGEDRTEKS 360

Query: 361 WRKPESPPPQEYPESRPES 364
           WRKPES P QE  ESRP+S
Sbjct: 361 WRKPES-PLQESLESRPDS 377

BLAST of CSPI07G00810 vs. TAIR 10
Match: AT4G38710.2 (glycine-rich protein )

HSP 1 Score: 279.6 bits (714), Expect = 3.7e-75
Identity = 197/396 (49.75%), Postives = 248/396 (62.63%), Query Frame = 0

Query: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKE---QEQQKQPELSLDFPSLNDALATK-QKKK 60
           MAA VSS W KPGAWAL++EEH+A+L ++     Q+   E S DFPSL  A  TK +KKK
Sbjct: 1   MAAAVSSVWAKPGAWALEAEEHEAELKQQPSPTNQKSSAEDSSDFPSLAAAATTKTKKKK 60

Query: 61  AQTLSLAEFNTYATTGSR---------SEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGS 120
            QT+SLAEF TY T  ++           + + LPTGPR+R+AEELDR++LGGGFR+YG 
Sbjct: 61  GQTISLAEFATYGTAKAKPAPQTERLTQAELVALPTGPRERSAEELDRSKLGGGFRSYG- 120

Query: 121 NGSYERNSRYSNADDSSNSKWGSSRVSDENRRSS---NR-ADSSKEFTSSRADEVDNWAL 180
                   RY   D+SS+S+WGSSRVS++  R     NR  + S++   SRADE DNWA 
Sbjct: 121 ------GGRY--GDESSSSRWGSSRVSEDGERRGGGFNRDREPSRDSGPSRADEDDNWAA 180

Query: 181 TKKPMAGNGFERRERGL--GFSDSQS-SKADESESWVSNKSNIPSDGRRF--------DR 240
            KKP++GNGFERRERG   GF +SQS SKADE +SWVS K   PS+ RRF        DR
Sbjct: 181 AKKPISGNGFERRERGSGGGFFESQSQSKADEVDSWVSTK---PSEPRRFVSSNGGGGDR 240

Query: 241 -ERRGGFTA----------SGGGADSDNWGRKKEE-----GNTGGVISSRPKLSLQPRTL 300
            E+RG F +           GGG++SD WGR++EE     G+      SRP+L LQPRTL
Sbjct: 241 FEKRGSFESLSRNRDSQYGGGGGSESDTWGRRREESGAANGSPPPSGGSRPRLVLQPRTL 300

Query: 301 PV----VDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKLDEQLES-KIKEVS 348
           PV    V    S +     + K +NPFG ARPREEVLAEKG DWK++DE+LE+ K+K+++
Sbjct: 301 PVAVVEVVKPESPVLVIVEKPKGANPFGNARPREEVLAEKGQDWKEIDEKLEAEKLKDIA 360

BLAST of CSPI07G00810 vs. TAIR 10
Match: AT4G38710.1 (glycine-rich protein )

HSP 1 Score: 278.1 bits (710), Expect = 1.1e-74
Identity = 199/410 (48.54%), Postives = 252/410 (61.46%), Query Frame = 0

Query: 1   MAAMVSSPWGKPGAWALDSEEHDAQLLKE---QEQQKQPELSLDFPSLNDALATK-QKKK 60
           MAA VSS W KPGAWAL++EEH+A+L ++     Q+   E S DFPSL  A  TK +KKK
Sbjct: 1   MAAAVSSVWAKPGAWALEAEEHEAELKQQPSPTNQKSSAEDSSDFPSLAAAATTKTKKKK 60

Query: 61  AQTLSLAEFNTYATTGSR---------SEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGS 120
            QT+SLAEF TY T  ++           + + LPTGPR+R+AEELDR++LGGGFR+YG 
Sbjct: 61  GQTISLAEFATYGTAKAKPAPQTERLTQAELVALPTGPRERSAEELDRSKLGGGFRSYG- 120

Query: 121 NGSYERNSRYSNADDSSNSKWGSSRVSDENRRSS---NR-ADSSKEFTSSRADEVDNWAL 180
                   RY   D+SS+S+WGSSRVS++  R     NR  + S++   SRADE DNWA 
Sbjct: 121 ------GGRY--GDESSSSRWGSSRVSEDGERRGGGFNRDREPSRDSGPSRADEDDNWAA 180

Query: 181 TKKPMAGNGFERRERGL--GFSDSQS-SKADESESWVSNKSNIPSDGRRF--------DR 240
            KKP++GNGFERRERG   GF +SQS SKADE +SWVS K   PS+ RRF        DR
Sbjct: 181 AKKPISGNGFERRERGSGGGFFESQSQSKADEVDSWVSTK---PSEPRRFVSSNGGGGDR 240

Query: 241 -ERRGGFTA----------SGGGADSDNWGRKKEE-----GNTGGVISSRPKLSLQPRTL 300
            E+RG F +           GGG++SD WGR++EE     G+      SRP+L LQPRTL
Sbjct: 241 FEKRGSFESLSRNRDSQYGGGGGSESDTWGRRREESGAANGSPPPSGGSRPRLVLQPRTL 300

Query: 301 PV----VDGKSSEISATAARSKSSNPFGAARPREEVLAEKGHDWKKLDEQLES-KIKEVS 360
           PV    V    S +     + K +NPFG ARPREEVLAEKG DWK++DE+LE+ K+K+++
Sbjct: 301 PVAVVEVVKPESPVLVIVEKPKGANPFGNARPREEVLAEKGQDWKEIDEKLEAEKLKDIA 360

Query: 361 VEEKTEKSNPIFFRKRSFGNGNSRAAEDRTERSWRKPESPPPQEYPESRP 362
                EK N     K  FG GN R  E+R ERSWRK      ++  E  P
Sbjct: 361 A--AMEKPNEKSTGKMGFGLGNGRKDEERIERSWRKSTEHSEEDAQEEEP 396

BLAST of CSPI07G00810 vs. TAIR 10
Match: AT1G13020.1 (eukaryotic initiation factor 4B2 )

HSP 1 Score: 128.6 bits (322), Expect = 1.1e-29
Identity = 121/356 (33.99%), Postives = 164/356 (46.07%), Query Frame = 0

Query: 5   VSSPWGKPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALATKQKKKAQTLSLAE 64
           +S PWG  GAWA ++E  D +  +  E       S  FPSL +A   K  KK + ++L+E
Sbjct: 1   MSKPWGGIGAWADEAERADEE--QAAEATATAADSQSFPSLKEAATAKSSKKKKKMTLSE 60

Query: 65  FNTYATT-----GSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYGSNGSYERNSRYSN 124
           F   A T     G   E  + LPTGPRQR+ +E+   RLGGGF +YG   S     R S 
Sbjct: 61  FTKGAYTAPSSAGLTREQMLQLPTGPRQRSEDEMQPGRLGGGFSSYGGGRSSGPPGRMSR 120

Query: 125 ADDSSNSKWGS--------SRVSDENRRSSNRADSSKEFTSSRADEVDNWALTKKPM--- 184
             + S+  WG             D+ R S++R     +   SRADE D+W   KK +   
Sbjct: 121 DREDSDGSWGGGGGGRRSYGGFDDDQRGSNSRVSDFPQV--SRADEDDDWGKGKKSLPSF 180

Query: 185 -----------------AGNGFERRERGLGFSDSQS------SKADESESWVSNKSNIPS 244
                             G G      G G + + S      SKADE ++W + K+   +
Sbjct: 181 DQGRQGSRYGGGGGSFGGGGGGGAGSYGGGGAGAGSGGGGGFSKADEVDNWAAGKAKSST 240

Query: 245 DGRRFDRERRGGFTASGGGADSDNWGRKKEEGNTGGVISSRPKLSLQPRTLPVVDGKSSE 304
            G         GF  S  G + D W R       GGV   R +L  +PR     D + SE
Sbjct: 241 FG--------SGFRES--GPEPDRWARGVLPSG-GGVQEERRRLVFEPRK---ADTEVSE 300

Query: 305 ISATAARSKSSNPFGAARPREEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSNP 322
            + TA ++   +PFGAARPRE+VLAEKG DWKKLD  +E+K  + S     + S P
Sbjct: 301 -TPTAVKTSKPSPFGAARPREQVLAEKGLDWKKLDSDIEAKKGQTSRPSSAQSSRP 337

BLAST of CSPI07G00810 vs. TAIR 10
Match: AT3G26400.1 (eukaryotic translation initiation factor 4B1 )

HSP 1 Score: 109.4 bits (272), Expect = 6.6e-24
Identity = 116/338 (34.32%), Postives = 157/338 (46.45%), Query Frame = 0

Query: 5   VSSPWG--KPGAWALDSEEHDAQLLKEQEQQKQPELSLDFPSLNDALA----TKQKKKAQ 64
           +S  WG    GAWA ++E  D +   E         S  FPSL +A A    + + +K +
Sbjct: 1   MSKAWGGIGIGAWADEAERADEEQAAEATAATADTQS--FPSLREAAAATATSGKSRKMK 60

Query: 65  TLSLAEFNTYATT--------GSRSEDFIVLPTGPRQRTAEELDRNRLGGGFRNYG--SN 124
            +SL+EF T A T        G   ++ + LPTGPRQR+ EE+   RLGGGF +YG  S 
Sbjct: 61  KMSLSEFTTGAYTAPGGRNSVGLTQQEILQLPTGPRQRSEEEMQPGRLGGGFSSYGGRSG 120

Query: 125 GSYERNSRYSNADDSSNSKWGSSRV----SDENRRSSNRADSSKEFTSSRADEVDNWALT 184
           G   R+   S+   S     G  R      D++RR  N++  S     SRADEVD+W   
Sbjct: 121 GRIGRDRDDSDGSWSGGGGGGGRRPYGGGFDDDRR-GNQSRVSDFPQPSRADEVDDWGKE 180

Query: 185 KKPMAGNGFERRERGLGFSDSQSSKADESESWVSNKSNIPSDGRRFDRERRGGFTASGGG 244
           KKP+    F++  +G    D                      G  F     GG    GGG
Sbjct: 181 KKPLP--SFDQGRQGRYSGDG---------------GGFGGGGSGFG----GGGGGGGGG 240

Query: 245 A----DSDNWGRKKEE------------GNTGGVISSRPKLSLQPRTLPVVDGKSSEISA 304
                D DNWG  K +            G++G     R +L L+PR    V+   SE   
Sbjct: 241 LSRADDVDNWGAGKRQAPVRSSTFGSSFGDSGQ--EERRRLVLEPRK---VESGGSETPP 300

Query: 305 TAARSKSSNPFGAARPREEVLAEKGHDWKKLDEQLESK 307
              ++   NPFGAARPRE+VLAEKG DWKK+D ++E+K
Sbjct: 301 VVEKTSKPNPFGAARPREDVLAEKGLDWKKIDSEIEAK 309

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZP81.5e-7348.54Eukaryotic translation initiation factor 4B3 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9SAD71.5e-2833.99Eukaryotic translation initiation factor 4B2 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9AUJ74.8e-2732.19Eukaryotic translation initiation factor 4B1 OS=Triticum aestivum OX=4565 GN=EIF... [more]
Q9LIN59.3e-2334.32Eukaryotic translation initiation factor 4B1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0K4M11.4e-20799.48Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G009760 PE=4 SV=1[more]
A0A5A7U3A11.8e-19494.62Eukaryotic translation initiation factor 4B3 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3BJA33.8e-19293.85eukaryotic translation initiation factor 4B3 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1ERF56.7e-14975.74eukaryotic translation initiation factor 4B3 isoform X2 OS=Cucurbita moschata OX... [more]
A0A6J1EKJ04.1e-14671.86eukaryotic translation initiation factor 4B3 isoform X1 OS=Cucurbita moschata OX... [more]
Match NameE-valueIdentityDescription
XP_004144838.12.9e-20799.48eukaryotic translation initiation factor 4B3 [Cucumis sativus] >KGN43232.1 hypot... [more]
KAA0049734.13.7e-19494.62eukaryotic translation initiation factor 4B3 [Cucumis melo var. makuwa] >TYK1214... [more]
XP_008447975.17.8e-19293.85PREDICTED: eukaryotic translation initiation factor 4B3 [Cucumis melo][more]
XP_038887904.12.3e-17586.60eukaryotic translation initiation factor 4B3-like isoform X1 [Benincasa hispida][more]
XP_038887905.13.5e-16887.60eukaryotic translation initiation factor 4B3-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G38710.23.7e-7549.75glycine-rich protein [more]
AT4G38710.11.1e-7448.54glycine-rich protein [more]
AT1G13020.11.1e-2933.99eukaryotic initiation factor 4B2 [more]
AT3G26400.16.6e-2434.32eukaryotic translation initiation factor 4B1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010433Plant specific eukaryotic initiation factor 4BPFAMPF06273eIF-4Bcoord: 6..321
e-value: 1.3E-57
score: 196.0
IPR010433Plant specific eukaryotic initiation factor 4BPANTHERPTHR32091EUKARYOTIC TRANSLATION INITIATION FACTOR 4Bcoord: 1..350
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 183..204
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..39
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 74..387
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 132..161
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 332..349
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 285..322
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 107..131
NoneNo IPR availablePANTHERPTHR32091:SF17EUKARYOTIC TRANSLATION INITIATION FACTOR 4B3coord: 1..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G00810.1CSPI07G00810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006413 translational initiation
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003743 translation initiation factor activity