CSPI06G36030 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G36030
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptiontwo-pore potassium channel 3
LocationChr6: 29118708 .. 29123010 (+)
RNA-Seq ExpressionCSPI06G36030
SyntenyCSPI06G36030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAGAAATGCGATTCCCAATCACAGATTACATAACGTTGTCTTCACAGGTTGGGTTGGGCGATCCGTTGTCAATGATCAATCATCTGCCCCTTGCAAATGGCGCGTGAAATGGATTTCTTCTTCCTTTTTATCCGATCCCTTCTTCTTCTTCTTCCTCTGTTTTCTCTTTTTCTTTCTGTTTTCTCTTTTCTGTAATGTAATAATCCAACTGGGTTTCCTCCATTTTTCAACATTTTCAAACCTTTTAGCTTCTGGGTCCCAACAAAATACTTCTCTCTTCCTTGTTAACTCCCATTTGAGCTCTCACTTTCTTCATTTGACTTGGAATTCATTCCTCAAAATCCCTCCCCTGCCTCGGTTTCATTTTGACGTTCTTTGTTTCTGCAAGCCTGCTGGTTGTATTTTGTTCTCACAGGTCAACCATCCCCATATCTCAATGGAGAAGGAGCCTCTTCTTCCATATCTAAGCCCAAGAGGGAAACCGTCTCCCATACCTCCTCAATTATGTCCACTTCCTGAAAACGATGAAATTACTCTGCCTATGACGCCCACGGAGTTCAAAGACCGCTTGATCTTTGGTCCTTCATCGTCCCCACAAGACGCTTCTCCTATATTCGACGCTTTGACGCTATCTCTTTCCTCTTCGAGACCTTCTGCTTCATCTTCTCTTCAAGACCCATCTACACCTTTGGACCCAAGATCACAATCGCAGTCTCAACAGGCGTGGCTTGTGGATTCCAATTATACATGGCCCAAAAGCAACCTCCATCGCTCCAAAACTGCACCTGCCATGGCGGTCATCAACGATGTCAATCATTCGCAGGAGCCCAAGCCACAGTTTGGTAAACAGTCTATTATCCGCCAAGCGGTTGTTCTTCTAATTGTTTACTTATCATTGGGTGTGCTTATCTATTGGTTAAATCGTGATAATTTTTCGGCTCAAGAGACTCATCCTGTGGTCGATGCATTGTACTTTTGTATTGTAACGATGTGCACTATTGGGTATGGAGATATTACCCCCAATAGTACGTCGACAAAGTTGTTCTCAGTATTGTTTGTGTTGGTGGGATTTGGGTTTATAGATATATTGCTCTCTGGAATGGTTAGTTATGTGCTTGATTTGCAGGAGAGTTATTTGTTGAGGAATGTAAAGAGGGGGGTGAAGCGTGAATCTGGGAAGTCCTATATAATTGATGTGAAGAAGGGAAGAATGAGGATTAGAATGAAGGTGGCATTGGCCTTAGGTGTTGTGATCCTTTGTATAGGGGTTGGCATGGGTGTTATGCATTTTGTTGAGAACCTGGGATGGTTGGATTCATTTTATCTTTCAGTTATGTCTGTCACAACAGTTGGATATGGTGATCAGGCATTCAAGTCGATGACTGGTCGGATTTTTGCTTCAATCTGGTTGCTTGTATCGACCCTTGCTGTTGCTCGGGCCTTCCTTTATTTAGCGGAGGCAAGAGTAGATAAGCGGCATAGGATGATGGCAAAATGGATACTTGGTCAAGATATGACCGTTTCTGAGTTTCTTGCTGCTGATATTGACAACAATGGCTTTGTGAGGTACGCATTAAGATTATTCATTCAAACCCATTTAGAGAAGTTGCTTGGAAAATTGGATTTGATGTTACTGATTCTGATTGCCGAGAAGTTTTGCTTGCATTGTTTCATGAATATGCATGAGTATTTGTTGCATAAGTTTTATGAAACTTCCCATCTATTCTGGTATGTTTGAAGAGTGAGCCATTGGTTGAAAAAATCACGTTTTAATTGTGCATTCATGGTATCCCTCTTATGAATATGATCATATTAATGATTCTATGAGACTTTCTACTTTTATAGCATCCAACTCAGCCTTCTGTTTGGCATAGGTTTGATATTATTTATCAACTCAGTCTTCCCTCTCCCAAGTACCACGAACTAACATATACAAAAAAGGATACTCCCCCTCCGACTTTGTCTTTCTCTCTTTTGGCATCCAGTATCCCAACTCATGCCCATTATGCAATCTCAAAATCTTGTTGACAGTAGATTCTTCAAAATTATTACATTCAAGCCAACTCCCTCTCTGAGGCGGTACCATTTTCTAATAAATGGTCTATCAATAAAACATGCTAATTATATGAATCTTTTAGATAGAACGGAAGAAGGATTTTTTTTTTCCTTTTGTTTTTAATGAAAGGGTAGATACTTCGGACTTGGTTCATTTGAAGACTTCTACTTGGTGTATCACCTACACTTTTGCAATAATGATTCTCTTGTTTGATTCTGATATGACAATAATCAAAACTCATGTAGATGTAGAGAGGGTGTCTTCAAAGACTATAGACTTTGCAGAGGAAAGTGTTGGGATACCAGTAGGGAAGTATTAAAAATTATTCCTTAGTGATTAGTTGGTAAATTTGTTAGAAGTGAATGGTTATAGACGGAGTCAGGGAACAGAATGTAGGCAAATTTTAAGAGAACGTAGGCAAATTTTAAGAGAATGTAGGCAAATTTTAAGAGAATGTAGATGTCTCGGAGTGCTTAGAACTTCATAAATTTTTCTAACGTTAATAGCATATTTCCATTAGTTCTGCTCCTGAGCAGAAGTACTATACAGTTATTGTTTAGTCATGGGAAGATATTGTACAAATAAAAATTAGAAGAGCGTAGAAGGTCGTGAAAGCCTCCTCCTCAAACTATTCTAAATAGAAATCACCTCAGTTCGAAGCCGCATTTCATCAATATGTGATTTAACTGTTCAGAATGTGTTAGGCCACCAATAGTGCATCAGTATAGTAGGAGAGGAAAGAATAATAAGGGCAAGGGAATTGTAAGGGGTGAGGACCACTTAGGGAGAATAATATAAATATGGTCAAAGTACATTGAGTTAGTTAGAAATTGCCTAGTATCTTCTTGAGAGTGAGAGATAGTTAGTTAGGGACGATTGTCTGTGATTGTTCCTAGTTATTCTACTGTGATTTCGGTGTACGATCCATTACTATTACAATAAAGATCGATACCTTTCAGAATGTAGGTACAAAAGTTTCGCAATTGAATGAAATTTGTGTGTGTCGTTTTTTTCTTAAGGTCCTGTCCTGGCTTCTGAATAGTAATTAGTTCTTCATTTGATACAATTTGAAGAAAAGTGGTAACTGAGAAATCATGATATATTTCTTTGCCATTAGTCTTCTGGTAGGACCCTTTTTTCTTTTAACAAGAAATGACGAGTAATGGGTTTCTTCTGTACATGTTTTCACTGATGACGTTTGTTGTGCACTTGGGCTAACCTGTTGGAATCTTATTACATGTTTGGATGGATTAGGATTACCATAGTAATTATGGTGGTGGATTCACCGAAGTGAACAAGACATAAGATCTTTACCTCAACCATTTTATATTCTCTGGATTTACTCTGATTGAAACTCTACAAGATGATCTTGATCATTCAAAATTGTACTTGATTCTTCTTTTAACGTGTTCTTTATTCAATCTGTAATTCCATCTCTAGTTTGTTTCTAGTGCCAGTTTGAGTTCCGGCTTTCCTTCCCAATCATCCTATCCTTTTCAATTCCCCATATTCTTCTGCCCTCTTAAATATCCTAGTCTAGCAAGTTTGGCTAGTAGCTCATTTGGTACGCATATCATGTTCTCAATCAAGTAATATAATTTATTCTCCTTGCCTTCTGATAGTTTCCATTTTACTAGCCTTGCAAGCTCTCTTGTGGATGAACTGTCTTCCCATTTAGTTTGACATATAACAGACCATCAAAACTTGGTGAGCTCACTTTGTATAGTTTATGTTATCCCTTTTGCAGTAGGTCAGAATATGTGATATACAAGCTGAAGGAGATGGGTAAGGTATCAGAGAAAGATGTCATGCAGATATCTAATAACTTTGATAGGCTAGACAGTGGCAAGTGTGGGAAGATTACACTGGCTGATCTCTTGGAAAGTCATCACTGATGTGAAAATACGATACTGTCAACGAAGAACTCGAGTTATGGGTCATCACAAATTGAAAGCAATTTCACTTTCAAATTGCAGATGCATAGTTGAAAGTGCATATATCTGGGATGTGCAAAGAAAGCCCTGAAAGAGAGTTTGCATTTCCCGGAACAACTATTTACTGCTCTTTCATCAGATGATTCTGTACAAAGTTTTGATAGCATAGGAAATATTCTTCTATAGAAGCTAAATGAATAATTGTTTGAACTTGTTGGACTGTAATTTGTTTATTGGTTTATACCTTTGTATGTATGTGAGCATGGGATGTCGCTCTTGAATATAAATGTCCATAGCTTCCTTTTCCTTTT

mRNA sequence

GGAAGAAATGCGATTCCCAATCACAGATTACATAACGTTGTCTTCACAGGTTGGGTTGGGCGATCCGTTGTCAATGATCAATCATCTGCCCCTTGCAAATGGCGCGTGAAATGGATTTCTTCTTCCTTTTTATCCGATCCCTTCTTCTTCTTCTTCCTCTGTTTTCTCTTTTTCTTTCTGTTTTCTCTTTTCTGTAATGTAATAATCCAACTGGGTTTCCTCCATTTTTCAACATTTTCAAACCTTTTAGCTTCTGGGTCCCAACAAAATACTTCTCTCTTCCTTGTTAACTCCCATTTGAGCTCTCACTTTCTTCATTTGACTTGGAATTCATTCCTCAAAATCCCTCCCCTGCCTCGGTTTCATTTTGACGTTCTTTGTTTCTGCAAGCCTGCTGGTTGTATTTTGTTCTCACAGGTCAACCATCCCCATATCTCAATGGAGAAGGAGCCTCTTCTTCCATATCTAAGCCCAAGAGGGAAACCGTCTCCCATACCTCCTCAATTATGTCCACTTCCTGAAAACGATGAAATTACTCTGCCTATGACGCCCACGGAGTTCAAAGACCGCTTGATCTTTGGTCCTTCATCGTCCCCACAAGACGCTTCTCCTATATTCGACGCTTTGACGCTATCTCTTTCCTCTTCGAGACCTTCTGCTTCATCTTCTCTTCAAGACCCATCTACACCTTTGGACCCAAGATCACAATCGCAGTCTCAACAGGCGTGGCTTGTGGATTCCAATTATACATGGCCCAAAAGCAACCTCCATCGCTCCAAAACTGCACCTGCCATGGCGGTCATCAACGATGTCAATCATTCGCAGGAGCCCAAGCCACAGTTTGGTAAACAGTCTATTATCCGCCAAGCGGTTGTTCTTCTAATTGTTTACTTATCATTGGGTGTGCTTATCTATTGGTTAAATCGTGATAATTTTTCGGCTCAAGAGACTCATCCTGTGGTCGATGCATTGTACTTTTGTATTGTAACGATGTGCACTATTGGGTATGGAGATATTACCCCCAATAGTACGTCGACAAAGTTGTTCTCAGTATTGTTTGTGTTGGTGGGATTTGGGTTTATAGATATATTGCTCTCTGGAATGGTTAGTTATGTGCTTGATTTGCAGGAGAGTTATTTGTTGAGGAATGTAAAGAGGGGGGTGAAGCGTGAATCTGGGAAGTCCTATATAATTGATGTGAAGAAGGGAAGAATGAGGATTAGAATGAAGGTGGCATTGGCCTTAGGTGTTGTGATCCTTTGTATAGGGGTTGGCATGGGTGTTATGCATTTTGTTGAGAACCTGGGATGGTTGGATTCATTTTATCTTTCAGTTATGTCTGTCACAACAGTTGGATATGGTGATCAGGCATTCAAGTCGATGACTGGTCGGATTTTTGCTTCAATCTGGTTGCTTGTATCGACCCTTGCTGTTGCTCGGGCCTTCCTTTATTTAGCGGAGGCAAGAGTAGATAAGCGGCATAGGATGATGGCAAAATGGATACTTGGTCAAGATATGACCGTTTCTGAGTTTCTTGCTGCTGATATTGACAACAATGGCTTTGTGAGTAGGTCAGAATATGTGATATACAAGCTGAAGGAGATGGGTAAGGTATCAGAGAAAGATGTCATGCAGATATCTAATAACTTTGATAGGCTAGACAGTGGCAAGTGTGGGAAGATTACACTGGCTGATCTCTTGGAAAGTCATCACTGATGTGAAAATACGATACTGTCAACGAAGAACTCGAGTTATGGGTCATCACAAATTGAAAGCAATTTCACTTTCAAATTGCAGATGCATAGTTGAAAGTGCATATATCTGGGATGTGCAAAGAAAGCCCTGAAAGAGAGTTTGCATTTCCCGGAACAACTATTTACTGCTCTTTCATCAGATGATTCTGTACAAAGTTTTGATAGCATAGGAAATATTCTTCTATAGAAGCTAAATGAATAATTGTTTGAACTTGTTGGACTGTAATTTGTTTATTGGTTTATACCTTTGTATGTATGTGAGCATGGGATGTCGCTCTTGAATATAAATGTCCATAGCTTCCTTTTCCTTTT

Coding sequence (CDS)

ATGGAGAAGGAGCCTCTTCTTCCATATCTAAGCCCAAGAGGGAAACCGTCTCCCATACCTCCTCAATTATGTCCACTTCCTGAAAACGATGAAATTACTCTGCCTATGACGCCCACGGAGTTCAAAGACCGCTTGATCTTTGGTCCTTCATCGTCCCCACAAGACGCTTCTCCTATATTCGACGCTTTGACGCTATCTCTTTCCTCTTCGAGACCTTCTGCTTCATCTTCTCTTCAAGACCCATCTACACCTTTGGACCCAAGATCACAATCGCAGTCTCAACAGGCGTGGCTTGTGGATTCCAATTATACATGGCCCAAAAGCAACCTCCATCGCTCCAAAACTGCACCTGCCATGGCGGTCATCAACGATGTCAATCATTCGCAGGAGCCCAAGCCACAGTTTGGTAAACAGTCTATTATCCGCCAAGCGGTTGTTCTTCTAATTGTTTACTTATCATTGGGTGTGCTTATCTATTGGTTAAATCGTGATAATTTTTCGGCTCAAGAGACTCATCCTGTGGTCGATGCATTGTACTTTTGTATTGTAACGATGTGCACTATTGGGTATGGAGATATTACCCCCAATAGTACGTCGACAAAGTTGTTCTCAGTATTGTTTGTGTTGGTGGGATTTGGGTTTATAGATATATTGCTCTCTGGAATGGTTAGTTATGTGCTTGATTTGCAGGAGAGTTATTTGTTGAGGAATGTAAAGAGGGGGGTGAAGCGTGAATCTGGGAAGTCCTATATAATTGATGTGAAGAAGGGAAGAATGAGGATTAGAATGAAGGTGGCATTGGCCTTAGGTGTTGTGATCCTTTGTATAGGGGTTGGCATGGGTGTTATGCATTTTGTTGAGAACCTGGGATGGTTGGATTCATTTTATCTTTCAGTTATGTCTGTCACAACAGTTGGATATGGTGATCAGGCATTCAAGTCGATGACTGGTCGGATTTTTGCTTCAATCTGGTTGCTTGTATCGACCCTTGCTGTTGCTCGGGCCTTCCTTTATTTAGCGGAGGCAAGAGTAGATAAGCGGCATAGGATGATGGCAAAATGGATACTTGGTCAAGATATGACCGTTTCTGAGTTTCTTGCTGCTGATATTGACAACAATGGCTTTGTGAGTAGGTCAGAATATGTGATATACAAGCTGAAGGAGATGGGTAAGGTATCAGAGAAAGATGTCATGCAGATATCTAATAACTTTGATAGGCTAGACAGTGGCAAGTGTGGGAAGATTACACTGGCTGATCTCTTGGAAAGTCATCACTGA

Protein sequence

MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIFDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMAVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADLLESHH*
Homology
BLAST of CSPI06G36030 vs. ExPASy Swiss-Prot
Match: Q9SVV6 (Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.1e-141
Identity = 282/429 (65.73%), Postives = 341/429 (79.49%), Query Frame = 0

Query: 4   EPLLPY-LSPRGKPSPIPPQLC-PLPENDE--ITLPMTPTEFKDRLIFGP-SSSPQDASP 63
           +PLL Y +SPR K    PPQL  PLPE++E  I +PMTP+EFK+RLIFGP S SP+D+S 
Sbjct: 7   DPLLQYMISPRLKK---PPQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRDSSH 66

Query: 64  IFDALTL-SLSSSRPSASSSLQDPST--PLDPRSQSQSQQAWLVDSNYTWPKSN-LHRSK 123
             D++   S SSS  + ++   D ST  PL P    Q  + WL D   +  + + LHRSK
Sbjct: 67  FIDSMKQPSPSSSSTAVNNPFSDSSTLDPLLPPPPPQ-PEPWLSDQTSSHCQGHALHRSK 126

Query: 124 TAPAMAVINDVNHS-QEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHP 183
           TAPAMAVIND++H  ++  P    +S++RQA  LL+VYLSLGVLIYWLNRD++   +THP
Sbjct: 127 TAPAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHP 186

Query: 184 VVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESY 243
           VVD LYFCIVTMCTIGYGDITPNS  TKLFS++FVLVGFGFIDILLSGMVSYVLDLQESY
Sbjct: 187 VVDGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESY 246

Query: 244 LLRNVKRGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLD 303
           +L + KR  + E  +SYIIDVKKGRMRIR+KVALALGVV+LCI VG+G+MHF+E +GWLD
Sbjct: 247 MLDSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLD 306

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFYLSVMSVTTVGYGD+AFK++ GR+FA+IWLLVSTLAVARAFLYLAEARVDKR+R  AK
Sbjct: 307 SFYLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAK 366

Query: 364 WILGQDMTVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCG 423
            +L + M+VS+F AADIDNNG VS++EYVIYKLKEM K+++KD++ IS  FD+LD    G
Sbjct: 367 KVLCETMSVSQFFAADIDNNGCVSKAEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNG 426

BLAST of CSPI06G36030 vs. ExPASy Swiss-Prot
Match: Q9FL25 (Two-pore potassium channel 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 3.4e-127
Identity = 254/430 (59.07%), Postives = 325/430 (75.58%), Query Frame = 0

Query: 4   EPLLPYL-SPRGKPSPIPPQLCPLPENDEITLPM--TPTEFKDRLIFGP-SSSPQDASPI 63
           +PL  YL +PR   +P PP L  LPEN+++T+PM  TP E K+RLIFG    S +++S  
Sbjct: 12  DPLRQYLMNPR--INPPPPSLLTLPENNDVTIPMPITPLELKNRLIFGSFVRSRKESSLP 71

Query: 64  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 123
            DAL+ + S+S  SA++S  D +  L P ++             + P  N HRSKTAPAM
Sbjct: 72  IDALSQNPSTS-SSATTSFSDSTDLLLPLTEPNK------PVRKSKPTINFHRSKTAPAM 131

Query: 124 AVINDVNHSQEPK--PQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDA 183
           A IN+++H  +PK   Q   ++I+ QAV LL+VYLSLGVLIYWLNRD+++ ++THPVVDA
Sbjct: 132 AAINNISHPNDPKTDQQSDSKTIVNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVDA 191

Query: 184 LYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRN 243
           LYFCIVTMCTIGYGDITP+S  TKLFS+ FVLVGFGF+DILLSGMV+YVLDLQE+Y+L  
Sbjct: 192 LYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLET 251

Query: 244 VKRGV----KRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLD 303
            +        R+  +SYIIDVKKGRMRIR+KV LALGVV+LC+G G+ +MHFVE +GWLD
Sbjct: 252 ARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLD 311

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFY SVMSVTTVGYGD+AF ++ GR+ A++WLLVSTLAVARA L+LAE+RVDKR+R  AK
Sbjct: 312 SFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERAK 371

Query: 364 WILGQDMTVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCG 423
            +LG+ M++S+FL ADID NG VS++E+VIYKLK+M K++EKD+  I   FD+LD    G
Sbjct: 372 KVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDKITEKDINPIGFQFDKLDRTNSG 431

BLAST of CSPI06G36030 vs. ExPASy Swiss-Prot
Match: Q69TN4 (Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE=3 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 1.9e-117
Identity = 244/451 (54.10%), Postives = 306/451 (67.85%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITL---PMTP-----TEFKDRLIFG---- 60
           M+ EPLL  LSP       P  L PLPE+ E++    P++P     + +K+R+IFG    
Sbjct: 1   MDTEPLLSPLSPS------PHLLHPLPEHAEVSTFSPPLSPCPSPASSYKERIIFGAHPP 60

Query: 61  ---PSSSPQDASPIFDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTW 120
              P   P    P        +S       S    PS P D  +   +  +     ++  
Sbjct: 61  PPPPPPPPPPPPPRGRRYYRRVSGDDLDVPSCSSSPSPPSDEENPPPNPPSLF---DFIG 120

Query: 121 PKSNLHRSKTAPAMAVINDV--------NHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVL 180
            ++NLHRS+TAPAMA +N            S+ P P   + +I+  A + L+ YL++GV 
Sbjct: 121 GRTNLHRSRTAPAMAPLNAAAIAAAAASGDSRNPPPPPRRPAIVLHAFLFLLAYLAMGVT 180

Query: 181 IYWLNRDNF--SAQETHPVVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFI 240
            Y     NF  SA  THPV DALYFCIVT+CTIGYGDITP + + KLFS+ FVL+GFGF+
Sbjct: 181 FYAALPGNFTSSAGPTHPVADALYFCIVTLCTIGYGDITPATPAAKLFSISFVLIGFGFV 240

Query: 241 DILLSGMVSYVLDLQESYLLRNVK--RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVI 300
           DILLSGMVSYVLDLQE  L+  +K  R V R+   +YI D+KKGRMR+RMKVALAL VV 
Sbjct: 241 DILLSGMVSYVLDLQEHLLITALKNPRSV-RKHRHNYIFDLKKGRMRVRMKVALALTVVA 300

Query: 301 LCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVA 360
           +C+GVG  V+  VENLGWLD+ YL+VMSVTTVGYGD AF+++ GR+FAS WLLVSTLAVA
Sbjct: 301 ICVGVGAAVLKRVENLGWLDAVYLAVMSVTTVGYGDHAFRTLAGRLFASAWLLVSTLAVA 360

Query: 361 RAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVS 420
           RAFLYLAE R+DKRHR MA W+L +DMTVSEFLAADIDNNG+V++SE+V+YKLKEMGK+S
Sbjct: 361 RAFLYLAEMRIDKRHRAMANWVLSRDMTVSEFLAADIDNNGYVTKSEFVVYKLKEMGKIS 420

Query: 421 EKDVMQISNNFDRLDSGKCGKITLADLLESH 425
           EKD+M I + F R+DSG CGKITL+DLLESH
Sbjct: 421 EKDIMMICDQFQRMDSGNCGKITLSDLLESH 441

BLAST of CSPI06G36030 vs. ExPASy Swiss-Prot
Match: Q9S6Z8 (Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 4.8e-105
Identity = 218/390 (55.90%), Postives = 274/390 (70.26%), Query Frame = 0

Query: 49  PSSSPQDASPIFDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKS 108
           PSSS   AS     +T+  S S  S    +      L  +   QS Q    D +    ++
Sbjct: 20  PSSSSSSAS-----ITIPRSISNTSFFHEISQERLLLHHQDLEQSVQDDKEDQDSDSDET 79

Query: 109 N--------LHRSKTAPAMAVINDVNHS--QEPKPQFGKQSIIRQAVVLLIVYLSLGVLI 168
           N        LHRS+TAPAM +I D+     +  KP    +SIIRQA+ LLIVYL+LGV I
Sbjct: 80  NRFLSQTRPLHRSRTAPAMVIIKDLRTKPPETKKPSPVSKSIIRQAIFLLIVYLTLGVSI 139

Query: 169 YWLNRDNFSAQETHPVVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDIL 228
           Y  NRD++S  ETHPVVDALYFCIVTMCTIGYGDI P +  TK+F+V+FVL GFGF+DIL
Sbjct: 140 YSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWTKIFAVVFVLFGFGFLDIL 199

Query: 229 LSGMVSYVLDLQESYLLRNVK-------RGVKRESGKSYIIDVKKGRMRIRMKVALALGV 288
           LSG+V+YVLDLQES +L  ++           R S K YIID +KGRMRIRMKV LAL V
Sbjct: 200 LSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIIDFEKGRMRIRMKVCLALCV 259

Query: 289 VILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLA 348
           V+LCIGVG  V+HFVE LG++DS YLSVMSVTTVGYGD+AFK++ GR+FA++WLLVSTLA
Sbjct: 260 VVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLA 319

Query: 349 VARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDNNGFVSRSEYVIYKLKEMGK 408
           VARAFLYLAEAR+D+RHR   K  L +++TV + L AD   +GF+S+SEY++ KLKEMGK
Sbjct: 320 VARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQHGFISKSEYIVLKLKEMGK 379

Query: 409 VSEKDVMQISNNFDRLDSGKCGKITLADLL 422
           +++KD+ Q+   F++LD  + GKITL DLL
Sbjct: 380 ITQKDIDQVVIQFEKLDPNQIGKITLPDLL 404

BLAST of CSPI06G36030 vs. ExPASy Swiss-Prot
Match: Q8LBL1 (Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 1.7e-54
Identity = 125/350 (35.71%), Postives = 207/350 (59.14%), Query Frame = 0

Query: 83  TPLDP--RSQSQSQQAWLVDSNYTWPKSNLHRSKTAP-AMAVINDVNHSQEPKPQFGKQS 142
           TPL P  +  + +Q   L     +  K  L RS++AP    + ND     EP P   K  
Sbjct: 8   TPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIP 67

Query: 143 I-------IRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYFCIVTMCTIGYGD 202
           +       +R+ ++ L +YL++G L ++L RD  S  +T  VVDALYFCIVTM T+GYGD
Sbjct: 68  MFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGD 127

Query: 203 ITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVKRESGKSYII 262
           + PNS++++L +  FV  G   +  LLS    Y+++ QE+ L+R     +++  G + I+
Sbjct: 128 LVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFH--LRQSFGPTDIL 187

Query: 263 -DVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQA 322
            ++   ++R +      + VV+  +G    VM  VE +  + +FY    +VTT+GYGD++
Sbjct: 188 KELHTNKLRYKCYATCLVLVVLFIVGTIFLVM--VEKMPVISAFYCVCSTVTTLGYGDKS 247

Query: 323 FKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADID 382
           F S  GR+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD+D
Sbjct: 248 FNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLD 307

Query: 383 NNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADLL 422
            +G V  +E+++YKLKEMGK+ EKD+  I + F++LD  + G +T +D++
Sbjct: 308 EDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

BLAST of CSPI06G36030 vs. ExPASy TrEMBL
Match: A0A1S3AYW0 (two-pore potassium channel 3 OS=Cucumis melo OX=3656 GN=LOC103484391 PE=3 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 4.4e-234
Identity = 419/425 (98.59%), Postives = 423/425 (99.53%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPYLSPRGKP P PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNF+AQETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNST+TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADL 420
           T+SEFLAADIDNNGFVSRSEYVIYKLKEMG+VSEKDVMQISNNFDRLDSGKCGKITLADL
Sbjct: 361 TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL 420

Query: 421 LESHH 426
           LESHH
Sbjct: 421 LESHH 425

BLAST of CSPI06G36030 vs. ExPASy TrEMBL
Match: A0A6J1ED61 (two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433251 PE=3 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 2.6e-223
Identity = 403/426 (94.60%), Postives = 413/426 (96.95%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPI 60
           MEKEPLLPY+SPRGKP P PPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYISPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL P+SQS SQQAWLVDSNY+W KSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD FSA ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS +TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 300
           RGV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLAD 420
           MTVSEFLAAD DNNG VSRSEYV+YKLKEMGKVSEKDVMQISNNFDRLD+G CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 426
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of CSPI06G36030 vs. ExPASy TrEMBL
Match: A0A6J1IME3 (two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111478359 PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 1.7e-222
Identity = 402/426 (94.37%), Postives = 412/426 (96.71%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPI 60
           MEKEPLLPY+SPRGKP P PPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL P+SQS SQQAWLVDSNY+W KSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALY 180
           AVINDVNHSQEPKPQFGK SIIRQAVVLLI+YLSLGVLIYW+NRD FSA ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKLSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS +TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 300
           RGV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLAD 420
           MTVSEFLAAD DNNG VSRSEYV+YKLKEMGKVSEKDVMQISNNFDRLD+G CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 426
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of CSPI06G36030 vs. ExPASy TrEMBL
Match: A0A6J1CLR2 (two-pore potassium channel 3-like OS=Momordica charantia OX=3673 GN=LOC111012441 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.2e-221
Identity = 399/425 (93.88%), Postives = 410/425 (96.47%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPY SPR K  P  P LCPLPENDEITLP+TPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYQSPRAKLPPPIPLLCPLPENDEITLPLTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120
           DALTLSLSSSR S SSSL DPSTPLDP+SQSQSQQAWLVDSNY WPK+NLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRSSPSSSLYDPSTPLDPKSQSQSQQAWLVDSNYAWPKTNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQE KPQFG QSIIRQAVVLLI+YLSLGVLIYW+N DNF+A ETHPVVDALYF
Sbjct: 121 VINDVNHSQERKPQFGNQSIIRQAVVLLIIYLSLGVLIYWINLDNFTAHETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNST+TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVE+LGWLDSFYLSVM
Sbjct: 241 GVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVEDLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKS+TGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSLTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADL 420
           TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLD+GKCGKITLADL
Sbjct: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDTGKCGKITLADL 420

Query: 421 LESHH 426
           LESHH
Sbjct: 421 LESHH 425

BLAST of CSPI06G36030 vs. ExPASy TrEMBL
Match: A0A6J1EM72 (two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433827 PE=3 SV=1)

HSP 1 Score: 756.1 bits (1951), Expect = 7.7e-215
Identity = 389/425 (91.53%), Postives = 406/425 (95.53%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           ME EPLLPY+SPRGKP P  PQL PLPENDEI LPMTPTEFKDRLIFGPSSSPQDAS  F
Sbjct: 1   METEPLLPYMSPRGKP-PSLPQLYPLPENDEIILPMTPTEFKDRLIFGPSSSPQDASSAF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120
           DALTLSL+S RPSASSS QDPSTPLDP+SQS SQQ WLVDS ++  KSNLHRSKTAPAMA
Sbjct: 61  DALTLSLASPRPSASSSRQDPSTPLDPKSQSPSQQGWLVDSKHSCHKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VIND+NHSQEPKPQFGKQSIIRQAVVLLI+YLSLGV+IYW+NRDNFSA ETHPVVDALYF
Sbjct: 121 VINDINHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSASETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNS +TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVE+LGWLDSFYLSVM
Sbjct: 241 GVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVEDLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADL 420
           TVSEFLAAD DNNG VSRSEYV+YKLKEMGKVSEKDVMQISNNFDRLD+GKCGKITLADL
Sbjct: 361 TVSEFLAADTDNNGCVSRSEYVVYKLKEMGKVSEKDVMQISNNFDRLDTGKCGKITLADL 420

Query: 421 LESHH 426
           L++HH
Sbjct: 421 LQNHH 424

BLAST of CSPI06G36030 vs. NCBI nr
Match: XP_004134597.1 (two-pore potassium channel 3 [Cucumis sativus])

HSP 1 Score: 827.4 bits (2136), Expect = 5.6e-236
Identity = 424/425 (99.76%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRS+TAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADL 420
           TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADL
Sbjct: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADL 420

Query: 421 LESHH 426
           LESHH
Sbjct: 421 LESHH 425

BLAST of CSPI06G36030 vs. NCBI nr
Match: XP_008439665.1 (PREDICTED: two-pore potassium channel 3 [Cucumis melo])

HSP 1 Score: 820.1 bits (2117), Expect = 9.0e-234
Identity = 419/425 (98.59%), Postives = 423/425 (99.53%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPYLSPRGKP P PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNF+AQETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNST+TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADL 420
           T+SEFLAADIDNNGFVSRSEYVIYKLKEMG+VSEKDVMQISNNFDRLDSGKCGKITLADL
Sbjct: 361 TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL 420

Query: 421 LESHH 426
           LESHH
Sbjct: 421 LESHH 425

BLAST of CSPI06G36030 vs. NCBI nr
Match: XP_038883350.1 (two-pore potassium channel 3-like [Benincasa hispida])

HSP 1 Score: 800.4 bits (2066), Expect = 7.4e-228
Identity = 410/425 (96.47%), Postives = 417/425 (98.12%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPY+SPRGKP P P QLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYVSPRGKPPPPPAQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLD +SQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDLKSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGV IYWLNRD FSA ETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVFIYWLNRDKFSAHETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNST+TKLFSVLFVLVGFGFIDILLSGMVSY+LDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYMLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           G++RESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGM VMHFVENLGWLDSFYLSVM
Sbjct: 241 GLQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMSVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADL 420
           TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLD+GKCGKITLADL
Sbjct: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDTGKCGKITLADL 420

Query: 421 LESHH 426
           LESHH
Sbjct: 421 LESHH 425

BLAST of CSPI06G36030 vs. NCBI nr
Match: XP_023544618.1 (two-pore potassium channel 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 784.3 bits (2024), Expect = 5.5e-223
Identity = 403/426 (94.60%), Postives = 413/426 (96.95%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPI 60
           MEKEPLLPY+SPRGKP P PPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL P+SQS SQQAWLVDSNY+W KSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD FSA ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS +TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 300
           RGV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLAD 420
           MTVSEFLAAD DNNG VSRSEYV+YKLKEMGKVSEKDVMQISNNFDRLD+G CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 426
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of CSPI06G36030 vs. NCBI nr
Match: XP_022926002.1 (two-pore potassium channel 3-like [Cucurbita moschata])

HSP 1 Score: 784.3 bits (2024), Expect = 5.5e-223
Identity = 403/426 (94.60%), Postives = 413/426 (96.95%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPI 60
           MEKEPLLPY+SPRGKP P PPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYISPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL P+SQS SQQAWLVDSNY+W KSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD FSA ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS +TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 300
           RGV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLAD 420
           MTVSEFLAAD DNNG VSRSEYV+YKLKEMGKVSEKDVMQISNNFDRLD+G CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 426
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of CSPI06G36030 vs. TAIR 10
Match: AT4G18160.1 (Ca2+ activated outward rectifying K+ channel 6 )

HSP 1 Score: 504.6 bits (1298), Expect = 7.8e-143
Identity = 282/429 (65.73%), Postives = 341/429 (79.49%), Query Frame = 0

Query: 4   EPLLPY-LSPRGKPSPIPPQLC-PLPENDE--ITLPMTPTEFKDRLIFGP-SSSPQDASP 63
           +PLL Y +SPR K    PPQL  PLPE++E  I +PMTP+EFK+RLIFGP S SP+D+S 
Sbjct: 7   DPLLQYMISPRLKK---PPQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRDSSH 66

Query: 64  IFDALTL-SLSSSRPSASSSLQDPST--PLDPRSQSQSQQAWLVDSNYTWPKSN-LHRSK 123
             D++   S SSS  + ++   D ST  PL P    Q  + WL D   +  + + LHRSK
Sbjct: 67  FIDSMKQPSPSSSSTAVNNPFSDSSTLDPLLPPPPPQ-PEPWLSDQTSSHCQGHALHRSK 126

Query: 124 TAPAMAVINDVNHS-QEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHP 183
           TAPAMAVIND++H  ++  P    +S++RQA  LL+VYLSLGVLIYWLNRD++   +THP
Sbjct: 127 TAPAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHP 186

Query: 184 VVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESY 243
           VVD LYFCIVTMCTIGYGDITPNS  TKLFS++FVLVGFGFIDILLSGMVSYVLDLQESY
Sbjct: 187 VVDGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESY 246

Query: 244 LLRNVKRGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLD 303
           +L + KR  + E  +SYIIDVKKGRMRIR+KVALALGVV+LCI VG+G+MHF+E +GWLD
Sbjct: 247 MLDSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLD 306

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFYLSVMSVTTVGYGD+AFK++ GR+FA+IWLLVSTLAVARAFLYLAEARVDKR+R  AK
Sbjct: 307 SFYLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAK 366

Query: 364 WILGQDMTVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCG 423
            +L + M+VS+F AADIDNNG VS++EYVIYKLKEM K+++KD++ IS  FD+LD    G
Sbjct: 367 KVLCETMSVSQFFAADIDNNGCVSKAEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNG 426

BLAST of CSPI06G36030 vs. TAIR 10
Match: AT5G46370.1 (Ca2+ activated outward rectifying K+ channel 2 )

HSP 1 Score: 456.4 bits (1173), Expect = 2.4e-128
Identity = 254/430 (59.07%), Postives = 325/430 (75.58%), Query Frame = 0

Query: 4   EPLLPYL-SPRGKPSPIPPQLCPLPENDEITLPM--TPTEFKDRLIFGP-SSSPQDASPI 63
           +PL  YL +PR   +P PP L  LPEN+++T+PM  TP E K+RLIFG    S +++S  
Sbjct: 12  DPLRQYLMNPR--INPPPPSLLTLPENNDVTIPMPITPLELKNRLIFGSFVRSRKESSLP 71

Query: 64  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 123
            DAL+ + S+S  SA++S  D +  L P ++             + P  N HRSKTAPAM
Sbjct: 72  IDALSQNPSTS-SSATTSFSDSTDLLLPLTEPNK------PVRKSKPTINFHRSKTAPAM 131

Query: 124 AVINDVNHSQEPK--PQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDA 183
           A IN+++H  +PK   Q   ++I+ QAV LL+VYLSLGVLIYWLNRD+++ ++THPVVDA
Sbjct: 132 AAINNISHPNDPKTDQQSDSKTIVNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVDA 191

Query: 184 LYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRN 243
           LYFCIVTMCTIGYGDITP+S  TKLFS+ FVLVGFGF+DILLSGMV+YVLDLQE+Y+L  
Sbjct: 192 LYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLET 251

Query: 244 VKRGV----KRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLD 303
            +        R+  +SYIIDVKKGRMRIR+KV LALGVV+LC+G G+ +MHFVE +GWLD
Sbjct: 252 ARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLD 311

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFY SVMSVTTVGYGD+AF ++ GR+ A++WLLVSTLAVARA L+LAE+RVDKR+R  AK
Sbjct: 312 SFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERAK 371

Query: 364 WILGQDMTVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCG 423
            +LG+ M++S+FL ADID NG VS++E+VIYKLK+M K++EKD+  I   FD+LD    G
Sbjct: 372 KVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDKITEKDINPIGFQFDKLDRTNSG 431

BLAST of CSPI06G36030 vs. TAIR 10
Match: AT4G01840.1 (Ca2+ activated outward rectifying K+ channel 5 )

HSP 1 Score: 382.9 bits (982), Expect = 3.4e-106
Identity = 218/390 (55.90%), Postives = 274/390 (70.26%), Query Frame = 0

Query: 49  PSSSPQDASPIFDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKS 108
           PSSS   AS     +T+  S S  S    +      L  +   QS Q    D +    ++
Sbjct: 20  PSSSSSSAS-----ITIPRSISNTSFFHEISQERLLLHHQDLEQSVQDDKEDQDSDSDET 79

Query: 109 N--------LHRSKTAPAMAVINDVNHS--QEPKPQFGKQSIIRQAVVLLIVYLSLGVLI 168
           N        LHRS+TAPAM +I D+     +  KP    +SIIRQA+ LLIVYL+LGV I
Sbjct: 80  NRFLSQTRPLHRSRTAPAMVIIKDLRTKPPETKKPSPVSKSIIRQAIFLLIVYLTLGVSI 139

Query: 169 YWLNRDNFSAQETHPVVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDIL 228
           Y  NRD++S  ETHPVVDALYFCIVTMCTIGYGDI P +  TK+F+V+FVL GFGF+DIL
Sbjct: 140 YSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWTKIFAVVFVLFGFGFLDIL 199

Query: 229 LSGMVSYVLDLQESYLLRNVK-------RGVKRESGKSYIIDVKKGRMRIRMKVALALGV 288
           LSG+V+YVLDLQES +L  ++           R S K YIID +KGRMRIRMKV LAL V
Sbjct: 200 LSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIIDFEKGRMRIRMKVCLALCV 259

Query: 289 VILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLA 348
           V+LCIGVG  V+HFVE LG++DS YLSVMSVTTVGYGD+AFK++ GR+FA++WLLVSTLA
Sbjct: 260 VVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLA 319

Query: 349 VARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDNNGFVSRSEYVIYKLKEMGK 408
           VARAFLYLAEAR+D+RHR   K  L +++TV + L AD   +GF+S+SEY++ KLKEMGK
Sbjct: 320 VARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQHGFISKSEYIVLKLKEMGK 379

Query: 409 VSEKDVMQISNNFDRLDSGKCGKITLADLL 422
           +++KD+ Q+   F++LD  + GKITL DLL
Sbjct: 380 ITQKDIDQVVIQFEKLDPNQIGKITLPDLL 404

BLAST of CSPI06G36030 vs. TAIR 10
Match: AT5G55630.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 214.9 bits (546), Expect = 1.2e-55
Identity = 125/350 (35.71%), Postives = 207/350 (59.14%), Query Frame = 0

Query: 83  TPLDP--RSQSQSQQAWLVDSNYTWPKSNLHRSKTAP-AMAVINDVNHSQEPKPQFGKQS 142
           TPL P  +  + +Q   L     +  K  L RS++AP    + ND     EP P   K  
Sbjct: 8   TPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIP 67

Query: 143 I-------IRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYFCIVTMCTIGYGD 202
           +       +R+ ++ L +YL++G L ++L RD  S  +T  VVDALYFCIVTM T+GYGD
Sbjct: 68  MFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGD 127

Query: 203 ITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVKRESGKSYII 262
           + PNS++++L +  FV  G   +  LLS    Y+++ QE+ L+R     +++  G + I+
Sbjct: 128 LVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFH--LRQSFGPTDIL 187

Query: 263 -DVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQA 322
            ++   ++R +      + VV+  +G    VM  VE +  + +FY    +VTT+GYGD++
Sbjct: 188 KELHTNKLRYKCYATCLVLVVLFIVGTIFLVM--VEKMPVISAFYCVCSTVTTLGYGDKS 247

Query: 323 FKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADID 382
           F S  GR+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD+D
Sbjct: 248 FNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLD 307

Query: 383 NNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADLL 422
            +G V  +E+++YKLKEMGK+ EKD+  I + F++LD  + G +T +D++
Sbjct: 308 EDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

BLAST of CSPI06G36030 vs. TAIR 10
Match: AT5G55630.2 (Outward rectifying potassium channel protein )

HSP 1 Score: 214.9 bits (546), Expect = 1.2e-55
Identity = 125/350 (35.71%), Postives = 207/350 (59.14%), Query Frame = 0

Query: 83  TPLDP--RSQSQSQQAWLVDSNYTWPKSNLHRSKTAP-AMAVINDVNHSQEPKPQFGKQS 142
           TPL P  +  + +Q   L     +  K  L RS++AP    + ND     EP P   K  
Sbjct: 8   TPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIP 67

Query: 143 I-------IRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYFCIVTMCTIGYGD 202
           +       +R+ ++ L +YL++G L ++L RD  S  +T  VVDALYFCIVTM T+GYGD
Sbjct: 68  MFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGD 127

Query: 203 ITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVKRESGKSYII 262
           + PNS++++L +  FV  G   +  LLS    Y+++ QE+ L+R     +++  G + I+
Sbjct: 128 LVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFH--LRQSFGPTDIL 187

Query: 263 -DVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQA 322
            ++   ++R +      + VV+  +G    VM  VE +  + +FY    +VTT+GYGD++
Sbjct: 188 KELHTNKLRYKCYATCLVLVVLFIVGTIFLVM--VEKMPVISAFYCVCSTVTTLGYGDKS 247

Query: 323 FKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADID 382
           F S  GR+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD+D
Sbjct: 248 FNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLD 307

Query: 383 NNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADLL 422
            +G V  +E+++YKLKEMGK+ EKD+  I + F++LD  + G +T +D++
Sbjct: 308 EDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SVV61.1e-14165.73Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1[more]
Q9FL253.4e-12759.07Two-pore potassium channel 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1[more]
Q69TN41.9e-11754.10Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE... [more]
Q9S6Z84.8e-10555.90Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1[more]
Q8LBL11.7e-5435.71Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3AYW04.4e-23498.59two-pore potassium channel 3 OS=Cucumis melo OX=3656 GN=LOC103484391 PE=3 SV=1[more]
A0A6J1ED612.6e-22394.60two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433251 ... [more]
A0A6J1IME31.7e-22294.37two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111478359 PE... [more]
A0A6J1CLR24.2e-22193.88two-pore potassium channel 3-like OS=Momordica charantia OX=3673 GN=LOC111012441... [more]
A0A6J1EM727.7e-21591.53two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433827 ... [more]
Match NameE-valueIdentityDescription
XP_004134597.15.6e-23699.76two-pore potassium channel 3 [Cucumis sativus][more]
XP_008439665.19.0e-23498.59PREDICTED: two-pore potassium channel 3 [Cucumis melo][more]
XP_038883350.17.4e-22896.47two-pore potassium channel 3-like [Benincasa hispida][more]
XP_023544618.15.5e-22394.60two-pore potassium channel 3-like [Cucurbita pepo subsp. pepo][more]
XP_022926002.15.5e-22394.60two-pore potassium channel 3-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G18160.17.8e-14365.73Ca2+ activated outward rectifying K+ channel 6 [more]
AT5G46370.12.4e-12859.07Ca2+ activated outward rectifying K+ channel 2 [more]
AT4G01840.13.4e-10655.90Ca2+ activated outward rectifying K+ channel 5 [more]
AT5G55630.11.2e-5535.71Outward rectifying potassium channel protein [more]
AT5G55630.21.2e-5535.71Outward rectifying potassium channel protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003280Two pore domain potassium channelPRINTSPR013332POREKCHANELcoord: 183..211
score: 35.03
coord: 303..312
score: 47.04
IPR003280Two pore domain potassium channelPANTHERPTHR11003POTASSIUM CHANNEL, SUBFAMILY Kcoord: 90..420
NoneNo IPR availableGENE3D1.10.287.70coord: 137..237
e-value: 2.1E-21
score: 77.6
NoneNo IPR availableGENE3D1.10.238.10coord: 334..425
e-value: 5.1E-6
score: 28.4
NoneNo IPR availableGENE3D1.10.287.70coord: 270..333
e-value: 1.4E-13
score: 52.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..93
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR11003:SF282TWO-PORE POTASSIUM CHANNEL 3coord: 90..420
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 255..361
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 140..226
IPR013099Potassium channel domainPFAMPF07885Ion_trans_2coord: 147..226
e-value: 8.0E-16
score: 57.8
coord: 272..340
e-value: 5.0E-11
score: 42.4
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 369..381
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 363..422

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G36030.1CSPI06G36030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071805 potassium ion transmembrane transport
biological_process GO:0030322 stabilization of membrane potential
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022841 potassium ion leak channel activity
molecular_function GO:0005267 potassium channel activity