CSPI06G33170 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G33170
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	1-aminocyclopropane-1-carboxylate oxidase
Location	Chr6: 27647778 .. 27649376 (-)
RNA-Seq Expression	CSPI06G33170
Synteny	CSPI06G33170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTCTCCACTATCTTATCTTTTTTTCTTTTCTTTTCATTTGTTAATTTCCCACTCCCTTGATCCCGTTCCCCCACGCCTATAAATACCATCCATTTCCATCTCCCACAACACACTCAGCAGAATTACAATAAGTTCTAGAGAGAAACAATGGAGATGGATTTCCCTGTCATCAACATGAACAACCTCAATGGCGAAAGCAGAGTATCTGTCCTGAACCAAATCAACGACGCCTGCGAAAACTGGGGTTTTTTTGAGGTAAATATCTTTATAAATTCATCATATATAAACTCTTTGGCTTAAGGTTCTGGGTTATTTATTTATTTACTTTTTTTTTTCTTTTGAAGTTGGTGAATCATGGGATACCACATGAGCTGATGGACAAAGTGGAGAAGATGACAAAGGAACATTATAGAAAGTGCATGGAGCAGAGGTTTAAAGAAATGGTAGCTTCCAAAGGGTTGGATTCAGTGGAAACTGAGATCAACGACACTGATTGGGAAAGCACTTTTTTTTTACGCCATCTTCCAGTTTCAAACATGTCAGAAATTGGTGATCTGGATGAGGAGTACAAGAAGGTGATGAAGGAATTTGCAGCTGAACTGGAGAAATTAGCAGAGGAAATTCTGAACTTGTTGTGTGAGAATCTTGGGCTTGAAAAGGGGTATTTGAAAAAAGTGTTTTATGGATCAAAAGGCCCAAACTTTGGGACAAAAGTTAGCAATTACCCTCCATGTCCTAAACCTGAGCTTATTAAAGGACTTAGAGCCCATACCGATGCTGGTGGTCTTATTCTTCTGTTTCAAGATGATAAAGTGAGTGGGCTTCAGCTGCTCAAAGATGGAAAATGGGTTGATGTTCCTCCGATGCATCACTCCATTGTTATCAATTTAGGAGACCAGCTTGAAGTACGTGATGATCTTTTTAGTGTGTGTGCGTGCTTTTTGTTATTATTACTATTATTTGTTTGTTTGGTATTAACATTAGTTATCTTGTTTTTGATTGAAATAGGTAATAACAAATGGAAAATACAAGAGTGTCATGCACAGAGTGATAGCACAGGAGGATGGAAACAGAATGTCGATAGCATCATTCTACAATCCAGGAAACGACGCCGTAATCTATCCGGCGCCGGCGCTGGTCGAGGGAGAGCAGGAGAAAACCAAACTTTACCCAAAATTTGTGTTCGATGATTACATGAAGCTGTATATGGGGCTTAAGTTTCAAGCAAAAGAGCCAAGGTTTGAGGCCATGAAAGCCATGGAGTCCACCAATATCAATATGGGTCCAATTGCAACTGTCTGAAAAGTGAAAACAAATTTTAAAAAAGGATTTGGATTAATTGTGGGTGTTTTTATTATTACAAAAGAAACCAGGAACAAATTTTAGTGGTACATTAGTGATATGATATAATGTTTGGGTGTTGGGGAACAGTGTTTAGTCTGTATTTTTATTTTACTTCTTAAAACATATTATGATAGTGGTTTGGGAGCAATATGAAGGGTTTCTCTTTCTAGGTAATATAGTTAACTTAAAATAATAAATATGTGTCTTTATGTTTCTGGTTTTTATTCAGAATGGAAAATAGACTTACTTTGTA

mRNA sequence

CTTTCTCCACTATCTTATCTTTTTTTCTTTTCTTTTCATTTGTTAATTTCCCACTCCCTTGATCCCGTTCCCCCACGCCTATAAATACCATCCATTTCCATCTCCCACAACACACTCAGCAGAATTACAATAAGTTCTAGAGAGAAACAATGGAGATGGATTTCCCTGTCATCAACATGAACAACCTCAATGGCGAAAGCAGAGTATCTGTCCTGAACCAAATCAACGACGCCTGCGAAAACTGGGGTTTTTTTGAGTTGGTGAATCATGGGATACCACATGAGCTGATGGACAAAGTGGAGAAGATGACAAAGGAACATTATAGAAAGTGCATGGAGCAGAGGTTTAAAGAAATGGTAGCTTCCAAAGGGTTGGATTCAGTGGAAACTGAGATCAACGACACTGATTGGGAAAGCACTTTTTTTTTACGCCATCTTCCAGTTTCAAACATGTCAGAAATTGGTGATCTGGATGAGGAGTACAAGAAGGTGATGAAGGAATTTGCAGCTGAACTGGAGAAATTAGCAGAGGAAATTCTGAACTTGTTGTGTGAGAATCTTGGGCTTGAAAAGGGGTATTTGAAAAAAGTGTTTTATGGATCAAAAGGCCCAAACTTTGGGACAAAAGTTAGCAATTACCCTCCATGTCCTAAACCTGAGCTTATTAAAGGACTTAGAGCCCATACCGATGCTGGTGGTCTTATTCTTCTGTTTCAAGATGATAAAGTGAGTGGGCTTCAGCTGCTCAAAGATGGAAAATGGGTTGATGTTCCTCCGATGCATCACTCCATTGTTATCAATTTAGGAGACCAGCTTGAAGTAATAACAAATGGAAAATACAAGAGTGTCATGCACAGAGTGATAGCACAGGAGGATGGAAACAGAATGTCGATAGCATCATTCTACAATCCAGGAAACGACGCCGTAATCTATCCGGCGCCGGCGCTGGTCGAGGGAGAGCAGGAGAAAACCAAACTTTACCCAAAATTTGTGTTCGATGATTACATGAAGCTGTATATGGGGCTTAAGTTTCAAGCAAAAGAGCCAAGGTTTGAGGCCATGAAAGCCATGGAGTCCACCAATATCAATATGGGTCCAATTGCAACTGTCTGAAAAGTGAAAACAAATTTTAAAAAAGGATTTGGATTAATTGTGGGTGTTTTTATTATTACAAAAGAAACCAGGAACAAATTTTAGTGGTACATTAGTGATATGATATAATGTTTGGGTGTTGGGGAACAGTGTTTAGTCTGTATTTTTATTTTACTTCTTAAAACATATTATGATAGTGGTTTGGGAGCAATATGAAGGGTTTCTCTTTCTAGGTAATATAGTTAACTTAAAATAATAAATATGTGTCTTTATGTTTCTGGTTTTTATTCAGAATGGAAAATAGACTTACTTTGTA

Coding sequence (CDS)

ATGGAGATGGATTTCCCTGTCATCAACATGAACAACCTCAATGGCGAAAGCAGAGTATCTGTCCTGAACCAAATCAACGACGCCTGCGAAAACTGGGGTTTTTTTGAGTTGGTGAATCATGGGATACCACATGAGCTGATGGACAAAGTGGAGAAGATGACAAAGGAACATTATAGAAAGTGCATGGAGCAGAGGTTTAAAGAAATGGTAGCTTCCAAAGGGTTGGATTCAGTGGAAACTGAGATCAACGACACTGATTGGGAAAGCACTTTTTTTTTACGCCATCTTCCAGTTTCAAACATGTCAGAAATTGGTGATCTGGATGAGGAGTACAAGAAGGTGATGAAGGAATTTGCAGCTGAACTGGAGAAATTAGCAGAGGAAATTCTGAACTTGTTGTGTGAGAATCTTGGGCTTGAAAAGGGGTATTTGAAAAAAGTGTTTTATGGATCAAAAGGCCCAAACTTTGGGACAAAAGTTAGCAATTACCCTCCATGTCCTAAACCTGAGCTTATTAAAGGACTTAGAGCCCATACCGATGCTGGTGGTCTTATTCTTCTGTTTCAAGATGATAAAGTGAGTGGGCTTCAGCTGCTCAAAGATGGAAAATGGGTTGATGTTCCTCCGATGCATCACTCCATTGTTATCAATTTAGGAGACCAGCTTGAAGTAATAACAAATGGAAAATACAAGAGTGTCATGCACAGAGTGATAGCACAGGAGGATGGAAACAGAATGTCGATAGCATCATTCTACAATCCAGGAAACGACGCCGTAATCTATCCGGCGCCGGCGCTGGTCGAGGGAGAGCAGGAGAAAACCAAACTTTACCCAAAATTTGTGTTCGATGATTACATGAAGCTGTATATGGGGCTTAAGTTTCAAGCAAAAGAGCCAAGGTTTGAGGCCATGAAAGCCATGGAGTCCACCAATATCAATATGGGTCCAATTGCAACTGTCTGA

Protein sequence

MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQEDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFEAMKAMESTNINMGPIATV*

Homology

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BLAST of CSPI06G33170 vs. ExPASy Swiss-Prot
Match: P54847 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucumis melo OX=3656 GN=ACO3 PE=2 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 5.1e-184
Identity = 311/320 (97.19%), Postives = 317/320 (99.06%), Query Frame = 0


Query: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK 60
           MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGI HELMDKVEK+TKEHYRK
Sbjct: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRK 60

Query: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA 120
           CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFA 
Sbjct: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAD 120

Query: 121 ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180
           ELEKLAEE+L+LLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD
Sbjct: 121 ELEKLAEEVLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180

Query: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDDKVSGL +LKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDDKVSGLHVLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR 300
           EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLY+GLKFQAKEPR
Sbjct: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYVGLKFQAKEPR 300

Query: 301 FEAMKAMESTNINMGPIATV 321
           FEAMKAMESTN+NMGPIATV
Sbjct: 301 FEAMKAMESTNLNMGPIATV 320

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BLAST of CSPI06G33170 vs. ExPASy Swiss-Prot
Match: P31237 (1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO PE=2 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 2.3e-152
Identity = 253/317 (79.81%), Postives = 292/317 (92.11%), Query Frame = 0


Query: 5   FPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCMEQ 64
           FPVI+M  LNGE R   + +I DACENWGFFELVNHGI HELMD VE++TKEHY KCMEQ
Sbjct: 4   FPVIDMEKLNGEERAPTMEKIKDACENWGFFELVNHGISHELMDTVERLTKEHYNKCMEQ 63

Query: 65  RFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAELEK 124
           RFKEMVA+KGL++V++EIND DWESTFFLRHLPVSN+SEI DL+++++K MKEFA +LEK
Sbjct: 64  RFKEMVATKGLEAVQSEINDLDWESTFFLRHLPVSNISEIPDLEQDHRKAMKEFAEKLEK 123

Query: 125 LAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGGL 184
           LAE++L+LLCEN+GLEKGYLKK FYGSKGP FGTKVSNYPPCP+PELIKGLRAHTDAGG+
Sbjct: 124 LAEQLLDLLCENVGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPRPELIKGLRAHTDAGGI 183

Query: 185 ILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQEDGN 244
           ILLFQD+KVSGLQLLKDG+W+DVPPM HSIVIN+GDQLEVITNGKYKSVMHRVIAQ DGN
Sbjct: 184 ILLFQDNKVSGLQLLKDGEWIDVPPMKHSIVINIGDQLEVITNGKYKSVMHRVIAQPDGN 243

Query: 245 RMSIASFYNPGNDAVIYPAPALVEGEQEKTK-LYPKFVFDDYMKLYMGLKFQAKEPRFEA 304
           RMSIASFYNPG+DAV+YPAPALV+ E+++ K +YPKFVF+DYMKLY GLKFQAKEPRFEA
Sbjct: 244 RMSIASFYNPGSDAVMYPAPALVDKEEDQQKQVYPKFVFEDYMKLYAGLKFQAKEPRFEA 303

Query: 305 MKAMESTNINMGPIATV 321
           MKAME+  +N+GPIAT+
Sbjct: 304 MKAMENA-VNLGPIATI 319

[Close]

BLAST of CSPI06G33170 vs. ExPASy Swiss-Prot
Match: Q08506 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 PE=1 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 5.7e-151
Identity = 251/318 (78.93%), Postives = 287/318 (90.25%), Query Frame = 0


Query: 4   DFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCME 63
           +FP+I+++ +NG  R + +  I DACENWGFFELVNHGIP E+MD VEKMTK HY+KCME
Sbjct: 3   NFPIISLDKVNGVERAATMEMIKDACENWGFFELVNHGIPREVMDTVEKMTKGHYKKCME 62

Query: 64  QRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAELE 123
           QRFKE+VASK L+ V+ E+ D DWESTFFL+HLP+SN+SE+ DLDEEY++VM++FA  LE
Sbjct: 63  QRFKELVASKALEGVQAEVTDMDWESTFFLKHLPISNISEVPDLDEEYREVMRDFAKRLE 122

Query: 124 KLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGG 183
           KLAEE+L+LLCENLGLEKGYLK  FYGSKGPNFGTKVSNYPPCPKP+LIKGLRAHTDAGG
Sbjct: 123 KLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGG 182

Query: 184 LILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQEDG 243
           +ILLFQDDKVSGLQLLKDG+W+DVPPM HSIV+NLGDQLEVITNGKYKSVMHRVIAQ+DG
Sbjct: 183 IILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVMHRVIAQKDG 242

Query: 244 NRMSIASFYNPGNDAVIYPAPALVEGEQEKTK-LYPKFVFDDYMKLYMGLKFQAKEPRFE 303
            RMS+ASFYNPG+DAVIYPAPALVE E E+ K +YPKFVFDDYMKLY GLKFQAKEPRFE
Sbjct: 243 ARMSLASFYNPGSDAVIYPAPALVEKEAEENKQVYPKFVFDDYMKLYAGLKFQAKEPRFE 302

Query: 304 AMKAMESTNINMGPIATV 321
           AMKAME T++ M PIATV
Sbjct: 303 AMKAME-TDVKMDPIATV 319

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BLAST of CSPI06G33170 vs. ExPASy Swiss-Prot
Match: Q8S932 (1-aminocyclopropane-1-carboxylate oxidase OS=Diospyros kaki OX=35925 GN=DK-ACO1 PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 7.5e-151
Identity = 250/316 (79.11%), Postives = 284/316 (89.87%), Query Frame = 0


Query: 5   FPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCMEQ 64
           FPVINM  +NGE R + +  INDACENWGFFELVNHGIP ELMD VE++TK HY+KCMEQ
Sbjct: 4   FPVINMEKMNGEERAATMGLINDACENWGFFELVNHGIPPELMDTVERVTKAHYKKCMEQ 63

Query: 65  RFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAELEK 124
           RFKE+VASK L+ ++ E+ D DWEST+FLRHLP SN+SE+ DLDEEY++VMK+FA  LEK
Sbjct: 64  RFKELVASKALEGIQAEVTDMDWESTYFLRHLPQSNISEVPDLDEEYRRVMKDFAERLEK 123

Query: 125 LAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGGL 184
           LAE +L+LLCENLGLEKGYLKK FYG+KGPNFGTKV+NYPPCPK +LIKGLRAHTDAGG+
Sbjct: 124 LAEYLLDLLCENLGLEKGYLKKAFYGTKGPNFGTKVANYPPCPKADLIKGLRAHTDAGGI 183

Query: 185 ILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQEDGN 244
           ILLFQDDKVSGLQLLKD +W+DVPPM HSIVINLGDQLEVITNGKYKSV+HRV+AQ DG 
Sbjct: 184 ILLFQDDKVSGLQLLKDDQWIDVPPMKHSIVINLGDQLEVITNGKYKSVLHRVVAQTDGT 243

Query: 245 RMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFEAM 304
           RMSIASFYNPGNDAVIYPAPALVE E E+ ++YPKFVFDDYMKLY  LKFQAKEPRFEAM
Sbjct: 244 RMSIASFYNPGNDAVIYPAPALVEKEVEEKEVYPKFVFDDYMKLYAALKFQAKEPRFEAM 303

Query: 305 KAMESTNINMGPIATV 321
           KA+E+ N+N+GPIATV
Sbjct: 304 KAVEA-NVNLGPIATV 318

[Close]

BLAST of CSPI06G33170 vs. ExPASy Swiss-Prot
Match: Q08507 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 3.7e-150
Identity = 250/318 (78.62%), Postives = 285/318 (89.62%), Query Frame = 0


Query: 4   DFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCME 63
           +FP+IN+  LNG  R + +  I DACENWGFFELVNHGIPHE+MD VEK+TK HY+KCME
Sbjct: 3   NFPIINLEKLNGSERDATMEMIKDACENWGFFELVNHGIPHEVMDTVEKLTKGHYKKCME 62

Query: 64  QRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAELE 123
           QRFKE+VASKGL++V+ E+ D DWESTFFLRHLPVSN+SE+ DLD+EY++VM++FA  LE
Sbjct: 63  QRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNISEVPDLDDEYREVMRDFAKRLE 122

Query: 124 KLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGG 183
           KLAEE+L+LLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPPCPKP+LIKGLRAHTDAGG
Sbjct: 123 KLAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGG 182

Query: 184 LILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQEDG 243
           +ILLFQDDKVSGLQLLKDG+W+DVPPM HSIV+NLGDQLEVITNGKYKSV+HRVIAQ DG
Sbjct: 183 IILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRVIAQTDG 242

Query: 244 NRMSIASFYNPGNDAVIYPAPALVEGE--QEKTKLYPKFVFDDYMKLYMGLKFQAKEPRF 303
            RMS+ASFYNPG+DAVIYPAP LVE E  QE  ++YPKFVFDDYMKLY GLKFQAKEPRF
Sbjct: 243 TRMSLASFYNPGSDAVIYPAPTLVEKEADQECKQVYPKFVFDDYMKLYAGLKFQAKEPRF 302

Query: 304 EAMKAMESTNINMGPIAT 320
           EAMKA E+ ++   PIAT
Sbjct: 303 EAMKAREA-DVKSDPIAT 319

[Close]

BLAST of CSPI06G33170 vs. ExPASy TrEMBL
Match: A0A0A0KH90 (ACC oxidase 2 OS=Cucumis sativus OX=3659 GN=Csa_6G511860 PE=3 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 9.7e-186
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0


Query: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK 60
           MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK
Sbjct: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK 60

Query: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA 120
           CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA
Sbjct: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA 120

Query: 121 ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180
           ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD
Sbjct: 121 ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180

Query: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR 300
           EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR
Sbjct: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR 300

Query: 301 FEAMKAMESTNINMGPIATV 321
           FEAMKAMESTNINMGPIATV
Sbjct: 301 FEAMKAMESTNINMGPIATV 320

[Close]

BLAST of CSPI06G33170 vs. ExPASy TrEMBL
Match: O64946 (ACC oxidase 2 OS=Cucumis sativus OX=3659 GN=Cs-ACO2 PE=2 SV=2)

HSP 1 Score: 649.4 bits (1674), Expect = 7.7e-183
Identity = 315/318 (99.06%), Postives = 315/318 (99.06%), Query Frame = 0


Query: 3   MDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM 62
           MDFPVINMNNLNGESRVS LNQIND CENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM
Sbjct: 1   MDFPVINMNNLNGESRVSFLNQINDVCENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM 60

Query: 63  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAEL 122
           EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAEL
Sbjct: 61  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAEL 120

Query: 123 EKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 182
           EKLA EILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG
Sbjct: 121 EKLAXEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180

Query: 183 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 242
           GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED
Sbjct: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 240

Query: 243 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFE 302
           GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFE
Sbjct: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFE 300

Query: 303 AMKAMESTNINMGPIATV 321
           AMKAMESTNINMGPIATV
Sbjct: 301 AMKAMESTNINMGPIATV 318

[Close]

BLAST of CSPI06G33170 vs. ExPASy TrEMBL
Match: A0A1S3B069 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucumis melo OX=3656 GN=LOC103484645 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.2e-182
Identity = 313/320 (97.81%), Postives = 318/320 (99.38%), Query Frame = 0


Query: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK 60
           MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGI HELMDKVEK+TKEHYRK
Sbjct: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRK 60

Query: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA 120
           CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFA 
Sbjct: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAD 120

Query: 121 ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180
           ELEKLAEE+L+LLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD
Sbjct: 121 ELEKLAEEVLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180

Query: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR 300
           EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLY+GLKFQAKEPR
Sbjct: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYVGLKFQAKEPR 300

Query: 301 FEAMKAMESTNINMGPIATV 321
           FEAMKAMESTN+NMGPIATV
Sbjct: 301 FEAMKAMESTNLNMGPIATV 320

[Close]

BLAST of CSPI06G33170 vs. ExPASy TrEMBL
Match: A0A6J1EEI3 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucurbita moschata OX=3662 GN=LOC111433496 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 1.0e-166
Identity = 283/320 (88.44%), Postives = 303/320 (94.69%), Query Frame = 0


Query: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK 60
           MEMDFPVINM  LNGE R + + QINDACENWGFFELVNHGI HELMD+VEK+TKEHYR+
Sbjct: 17  MEMDFPVINMEKLNGEDRGAAMEQINDACENWGFFELVNHGISHELMDEVEKLTKEHYRQ 76

Query: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA 120
           CMEQ+F +MVAS+GLDSV+TEINDTDWESTFFLRHLPVSNMSEI DLDE+Y+KVMKEFA 
Sbjct: 77  CMEQKFNDMVASRGLDSVDTEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRKVMKEFAV 136

Query: 121 ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180
           ELEKLAE++L+LLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD
Sbjct: 137 ELEKLAEQLLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 196

Query: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQD KVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRV AQ
Sbjct: 197 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVKAQ 256

Query: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR 300
            DGNRMS+ASFYNPG+DAVIYPAPALVEGE+E +KLYPKFVF+DYMKLYMGLKFQAKEPR
Sbjct: 257 PDGNRMSLASFYNPGDDAVIYPAPALVEGEEESSKLYPKFVFNDYMKLYMGLKFQAKEPR 316

Query: 301 FEAMKAMESTNINMGPIATV 321
           FEAMKA ESTN+ MGPIATV
Sbjct: 317 FEAMKATESTNLKMGPIATV 336

[Close]

BLAST of CSPI06G33170 vs. ExPASy TrEMBL
Match: A0A6J1KXP7 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucurbita maxima OX=3661 GN=LOC111497285 PE=3 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.1e-165
Identity = 281/320 (87.81%), Postives = 303/320 (94.69%), Query Frame = 0


Query: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK 60
           MEMDFPVINM  LNGE R + + +INDACENWGFFELVNHGI HELMD+VEK+TKEHYRK
Sbjct: 17  MEMDFPVINMEKLNGEDRGAAMEKINDACENWGFFELVNHGISHELMDEVEKLTKEHYRK 76

Query: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA 120
           CMEQ+FK+MVAS+GLDSV+TEINDTDWESTFFLRHLPVSNMSEI DLDE+YKKVMKEFA 
Sbjct: 77  CMEQKFKDMVASRGLDSVDTEINDTDWESTFFLRHLPVSNMSEIPDLDEDYKKVMKEFAV 136

Query: 121 ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180
           ELEKLAE++L+LLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPPCPKPELI+GLRAHTD
Sbjct: 137 ELEKLAEQLLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIEGLRAHTD 196

Query: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQD KVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRV AQ
Sbjct: 197 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVKAQ 256

Query: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR 300
            DGNRMS+ASFYNPG+DAVIYPAPALVEGE+  +KLYPKFVF+DYMKLY+GLKFQAKEPR
Sbjct: 257 PDGNRMSLASFYNPGDDAVIYPAPALVEGEEASSKLYPKFVFNDYMKLYVGLKFQAKEPR 316

Query: 301 FEAMKAMESTNINMGPIATV 321
           FEAMKA +STN+ MGPIATV
Sbjct: 317 FEAMKATDSTNLQMGPIATV 336

[Close]

BLAST of CSPI06G33170 vs. NCBI nr
Match: KGN49070.1 (hypothetical protein Csa_004451 [Cucumis sativus])

HSP 1 Score: 659.1 bits (1699), Expect = 2.0e-185
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0


Query: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK 60
           MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK
Sbjct: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK 60

Query: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA 120
           CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA
Sbjct: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA 120

Query: 121 ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180
           ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD
Sbjct: 121 ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180

Query: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR 300
           EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR
Sbjct: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR 300

Query: 301 FEAMKAMESTNINMGPIATV 321
           FEAMKAMESTNINMGPIATV
Sbjct: 301 FEAMKAMESTNINMGPIATV 320

[Close]

BLAST of CSPI06G33170 vs. NCBI nr
Match: NP_001292672.1 (1-aminocyclopropane-1-carboxylate oxidase 3 [Cucumis sativus] >AAC67233.1 ACC oxidase 2 [Cucumis sativus])

HSP 1 Score: 649.4 bits (1674), Expect = 1.6e-182
Identity = 315/318 (99.06%), Postives = 315/318 (99.06%), Query Frame = 0


Query: 3   MDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM 62
           MDFPVINMNNLNGESRVS LNQIND CENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM
Sbjct: 1   MDFPVINMNNLNGESRVSFLNQINDVCENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM 60

Query: 63  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAEL 122
           EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAEL
Sbjct: 61  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAEL 120

Query: 123 EKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 182
           EKLA EILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG
Sbjct: 121 EKLAXEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180

Query: 183 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 242
           GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED
Sbjct: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 240

Query: 243 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFE 302
           GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFE
Sbjct: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFE 300

Query: 303 AMKAMESTNINMGPIATV 321
           AMKAMESTNINMGPIATV
Sbjct: 301 AMKAMESTNINMGPIATV 318

[Close]

BLAST of CSPI06G33170 vs. NCBI nr
Match: XP_008440054.1 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 3 [Cucumis melo])

HSP 1 Score: 647.9 bits (1670), Expect = 4.6e-182
Identity = 313/320 (97.81%), Postives = 318/320 (99.38%), Query Frame = 0


Query: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK 60
           MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGI HELMDKVEK+TKEHYRK
Sbjct: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRK 60

Query: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA 120
           CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFA 
Sbjct: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAD 120

Query: 121 ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180
           ELEKLAEE+L+LLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD
Sbjct: 121 ELEKLAEEVLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180

Query: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR 300
           EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLY+GLKFQAKEPR
Sbjct: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYVGLKFQAKEPR 300

Query: 301 FEAMKAMESTNINMGPIATV 321
           FEAMKAMESTN+NMGPIATV
Sbjct: 301 FEAMKAMESTNLNMGPIATV 320

[Close]

BLAST of CSPI06G33170 vs. NCBI nr
Match: P54847.1 (RecName: Full=1-aminocyclopropane-1-carboxylate oxidase 3; Short=ACC oxidase 3; AltName: Full=Ethylene-forming enzyme; Short=EFE [Cucumis melo] >CAA64799.1 ACC oxidase [Cucumis melo])

HSP 1 Score: 644.8 bits (1662), Expect = 3.9e-181
Identity = 311/320 (97.19%), Postives = 317/320 (99.06%), Query Frame = 0


Query: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK 60
           MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGI HELMDKVEK+TKEHYRK
Sbjct: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRK 60

Query: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA 120
           CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFA 
Sbjct: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAD 120

Query: 121 ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180
           ELEKLAEE+L+LLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD
Sbjct: 121 ELEKLAEEVLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180

Query: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDDKVSGL +LKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDDKVSGLHVLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR 300
           EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLY+GLKFQAKEPR
Sbjct: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYVGLKFQAKEPR 300

Query: 301 FEAMKAMESTNINMGPIATV 321
           FEAMKAMESTN+NMGPIATV
Sbjct: 301 FEAMKAMESTNLNMGPIATV 320

[Close]

BLAST of CSPI06G33170 vs. NCBI nr
Match: XP_038881182.1 (1-aminocyclopropane-1-carboxylate oxidase 3 [Benincasa hispida])

HSP 1 Score: 620.2 bits (1598), Expect = 1.0e-173
Identity = 296/318 (93.08%), Postives = 310/318 (97.48%), Query Frame = 0


Query: 3   MDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM 62
           M+FPVINMNNLNGE RVS + QINDACENWGFFELVNHGI HELMDKVEK+TKEHYRKCM
Sbjct: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60

Query: 63  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAEL 122
           EQRFKEMVA KGLDSVETEINDTDWESTFFLRHLPVSNMSEI DLDE+Y++VMKEFA EL
Sbjct: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120

Query: 123 EKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 182
           EKLAE+IL+LLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG
Sbjct: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180

Query: 183 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 242
           GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ+D
Sbjct: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240

Query: 243 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFE 302
           GNRMSIASFYNPGNDAVIYPAPALVEGE+EK+KLYPKFVFDDYMKLY+GLKFQAKEPRFE
Sbjct: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300

Query: 303 AMKAMESTNINMGPIATV 321
           AMKAMESTN+N+GPIATV
Sbjct: 301 AMKAMESTNLNLGPIATV 318

[Close]

BLAST of CSPI06G33170 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 504.2 bits (1297), Expect = 7.7e-143
Identity = 234/319 (73.35%), Postives = 282/319 (88.40%), Query Frame = 0


Query: 5   FPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCMEQ 64
           FP+IN+  LNGE R   + +I DACENWGFFE VNHGI  EL+DKVEKMTKEHY+KCME+
Sbjct: 4   FPIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKCMEE 63

Query: 65  RFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAELEK 124
           RFKE + ++GLDS+ +E+ND DWESTF+L+HLPVSN+S++ DLD++Y+ +MK+FA ++EK
Sbjct: 64  RFKESIKNRGLDSLRSEVNDVDWESTFYLKHLPVSNISDVPDLDDDYRTLMKDFAGKIEK 123

Query: 125 LAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGGL 184
           L+EE+L+LLCENLGLEKGYLKKVFYGSK P FGTKVSNYPPCP P+L+KGLRAHTDAGG+
Sbjct: 124 LSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPDLVKGLRAHTDAGGI 183

Query: 185 ILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQEDG- 244
           ILLFQDDKVSGLQLLKDG+WVDVPP+ HSIV+NLGDQLEVITNGKYKSV HRV++Q DG 
Sbjct: 184 ILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEHRVLSQTDGE 243

Query: 245 NRMSIASFYNPGNDAVIYPAPALV--EGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRF 304
            RMSIASFYNPG+D+VI+PAP L+  E E+EK + YP+FVF+DYMKLY  +KFQAKEPRF
Sbjct: 244 GRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAVKFQAKEPRF 303

Query: 305 EAMKAMESTNI-NMGPIAT 320
           EAMKAME+T   N+GP+AT
Sbjct: 304 EAMKAMETTVANNVGPLAT 322

[Close]

BLAST of CSPI06G33170 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 484.6 bits (1246), Expect = 6.3e-137
Identity = 221/318 (69.50%), Postives = 269/318 (84.59%), Query Frame = 0


Query: 3   MDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM 62
           M FPV++++ LNGE R   +  IN+ACENWGFFE+VNHG+PH+LMDK+EKMTK+HY+ C 
Sbjct: 5   MKFPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQ 64

Query: 63  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAEL 122
           EQ+F +M+ SKGLD++ETE+ D DWESTF++RHLP SN+++I D+ +EY+  MK+F   L
Sbjct: 65  EQKFNDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAMKDFGKRL 124

Query: 123 EKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 182
           E LAE++L+LLCENLGLEKGYLKKVF+G+KGP FGTKVSNYPPCPKPE+IKGLRAHTDAG
Sbjct: 125 ENLAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAHTDAG 184

Query: 183 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 242
           G+ILLFQDDKVSGLQLLKDG W+DVPP++HSIVINLGDQLEVITNGKYKSV+HRV+ Q++
Sbjct: 185 GIILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKYKSVLHRVVTQQE 244

Query: 243 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFE 302
           GNRMS+ASFYNPG+DA I PA +LVE + E    YP FVFDDYMKLY G+KFQ KEPRF 
Sbjct: 245 GNRMSVASFYNPGSDAEISPATSLVEKDSE----YPSFVFDDYMKLYAGVKFQPKEPRFA 304

Query: 303 AMKAMESTNINMGPIATV 321
           AMK   +    + P A V
Sbjct: 305 AMKNASAVT-ELNPTAAV 317

[Close]

BLAST of CSPI06G33170 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 481.1 bits (1237), Expect = 6.9e-136
Identity = 228/320 (71.25%), Postives = 269/320 (84.06%), Query Frame = 0


Query: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRK 60
           M + FPVI+++ LNGE R   +  I+DAC+NWGFFELVNHG+P++LMD +E+MTKEHY+K
Sbjct: 3   MNIKFPVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKK 62

Query: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAA 120
            MEQ+FKEM+ SKGLD++ETE+ D DWESTF+L HLP SN+ +I D+  EY+  MK+F  
Sbjct: 63  HMEQKFKEMLRSKGLDTLETEVEDVDWESTFYLHHLPQSNLYDIPDMSNEYRLAMKDFGK 122

Query: 121 ELEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180
            LE LAEE+L+LLCENLGLEKGYLKKVF+G+ GP F TK+SNYPPCPKPE+IKGLRAHTD
Sbjct: 123 RLEILAEELLDLLCENLGLEKGYLKKVFHGTTGPTFATKLSNYPPCPKPEMIKGLRAHTD 182

Query: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDDKVSGLQLLKDG WVDVPP+ HSIVINLGDQLEVITNGKYKSVMHRV+ Q
Sbjct: 183 AGGLILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMHRVMTQ 242

Query: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPR 300
           ++GNRMSIASFYNPG+DA I PA +LV    +K   YP FVFDDYMKLY GLKFQAKEPR
Sbjct: 243 KEGNRMSIASFYNPGSDAEISPATSLV----DKDSKYPSFVFDDYMKLYAGLKFQAKEPR 302

Query: 301 FEAMKAMESTNINMGPIATV 321
           FEAMK  E+   ++ P+A V
Sbjct: 303 FEAMKNAEAA-ADLNPVAVV 317

[Close]

BLAST of CSPI06G33170 vs. TAIR 10
Match: AT2G19590.1 (ACC oxidase 1 )

HSP 1 Score: 318.9 bits (816), Expect = 4.6e-87
Identity = 153/308 (49.68%), Postives = 210/308 (68.18%), Query Frame = 0


Query: 2   EMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKC 61
           EM+ PVI+   L+GE R   ++ ++ AC+ WGFF + NHGI  ELM+KV+KM   HY + 
Sbjct: 8   EMEIPVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEH 67

Query: 62  MEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAE 121
           ++++F +    K L   E + +D DWES+FF+ H P SN+ +I ++ EE  K M E+  +
Sbjct: 68  LKEKFYQSEMVKALS--EGKTSDADWESSFFISHKPTSNICQIPNISEELSKTMDEYVCQ 127

Query: 122 LEKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDA 181
           L K AE +  L+CENLGL++  +   F G KGP FGTKV+ YP CP+PEL++GLR HTDA
Sbjct: 128 LHKFAERLSKLMCENLGLDQEDIMNAFSGPKGPAFGTKVAKYPECPRPELMRGLREHTDA 187

Query: 182 GGLILLFQDDKVSGLQLLKDGKWVDVPP-MHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 241
           GG+ILL QDD+V GL+  KDGKWV +PP  +++I +N GDQLE+++NG+YKSV+HRV+  
Sbjct: 188 GGIILLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSVVHRVMTV 247

Query: 242 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPK-FVFDDYMKLYMGLKFQAKEP 301
           + G+R+SIA+FYNP  DA+I PAP L         LYP  + F DY+KLY   KF  K P
Sbjct: 248 KHGSRLSIATFYNPAGDAIISPAPKL---------LYPSGYRFQDYLKLYSTTKFGDKGP 304

Query: 302 RFEAMKAM 308
           R E MK M
Sbjct: 308 RLETMKKM 304

[Close]

BLAST of CSPI06G33170 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.1e-83
Identity = 159/315 (50.48%), Postives = 211/315 (66.98%), Query Frame = 0


Query: 3   MDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM 62
           M  PVI+ + LNGE R   L++I  ACE WGFF+LVNHGIP EL++KV+K++ + Y+   
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 63  EQRFKEMVASKGLDSV-----ETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKE 122
           E+ FK     K L+ +       ++ + DWE  F L      N +E        K+ M E
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLD---HNQNE---WPSNIKETMGE 120

Query: 123 FAAELEKLAEEILNLLCENLGLEKGYLKKVFY-----GSKGPNFGTKVSNYPPCPKPELI 182
           +  E+ KLA +++ ++ ENLGL KGY+KK F      G +   FGTKVS+YPPCP PEL+
Sbjct: 121 YREEVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELV 180

Query: 183 KGLRAHTDAGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKS 242
            GLRAHTDAGG++LLFQDD+  GLQ+LKDG+W+DV P+ ++IVIN GDQ+EV++NG+YKS
Sbjct: 181 NGLRAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKS 240

Query: 243 VMHRVIAQEDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGL 302
             HRV+A+E+GNR SIASFYNP   A I   PA V  E+   K YPKFVF DYM +Y   
Sbjct: 241 AWHRVLAREEGNRRSIASFYNPSYKAAI--GPAAVAEEEGSEKKYPKFVFGDYMDVYANQ 300

Query: 303 KFQAKEPRFEAMKAM 308
           KF  KEPRF A+K++
Sbjct: 301 KFMPKEPRFLAVKSL 307

The following BLAST results are available for this feature:

BLAST of CSPI06G33170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P54847	5.1e-184	97.19	1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucumis melo OX=3656 GN=ACO3 PE=2...	[more]
P31237	2.3e-152	79.81	1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO ...	[more]
Q08506	5.7e-151	78.93	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 P...	[more]
Q8S932	7.5e-151	79.11	1-aminocyclopropane-1-carboxylate oxidase OS=Diospyros kaki OX=35925 GN=DK-ACO1 ...	[more]
Q08507	3.7e-150	78.62	1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 P...	[more]

BLAST of CSPI06G33170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KH90	9.7e-186	100.00	ACC oxidase 2 OS=Cucumis sativus OX=3659 GN=Csa_6G511860 PE=3 SV=1	[more]
O64946	7.7e-183	99.06	ACC oxidase 2 OS=Cucumis sativus OX=3659 GN=Cs-ACO2 PE=2 SV=2	[more]
A0A1S3B069	2.2e-182	97.81	1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucumis melo OX=3656 GN=LOC103484...	[more]
A0A6J1EEI3	1.0e-166	88.44	1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1KXP7	1.1e-165	87.81	1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]

BLAST of CSPI06G33170 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN49070.1	2.0e-185	100.00	hypothetical protein Csa_004451 [Cucumis sativus]	[more]
NP_001292672.1	1.6e-182	99.06	1-aminocyclopropane-1-carboxylate oxidase 3 [Cucumis sativus] >AAC67233.1 ACC ox...	[more]
XP_008440054.1	4.6e-182	97.81	PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 3 [Cucumis melo]	[more]
P54847.1	3.9e-181	97.19	RecName: Full=1-aminocyclopropane-1-carboxylate oxidase 3; Short=ACC oxidase 3; ...	[more]
XP_038881182.1	1.0e-173	93.08	1-aminocyclopropane-1-carboxylate oxidase 3 [Benincasa hispida]	[more]

BLAST of CSPI06G33170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05010.1	7.7e-143	73.35	ethylene-forming enzyme	[more]
AT1G62380.1	6.3e-137	69.50	ACC oxidase 2	[more]
AT1G12010.1	6.9e-136	71.25	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G19590.1	4.6e-87	49.68	ACC oxidase 1	[more]
AT1G77330.1	1.1e-83	50.48	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 108..135
None	No IPR available	PANTHER	PTHR47990:SF63	ACC OXIDASE 2	coord: 2..307
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 2..307
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 3..305
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 6..95 e-value: 1.8E-14 score: 54.5
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 160..254 e-value: 1.2E-27 score: 96.3
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..320 e-value: 7.6E-103 score: 346.1
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 154..254 score: 13.141385

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G33170.1	CSPI06G33170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009693	ethylene biosynthetic process
biological_process	GO:0009835	fruit ripening
molecular_function	GO:0009815	1-aminocyclopropane-1-carboxylate oxidase activity
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0046872	metal ion binding