CSPI06G32380 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G32380
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	4-coumarate--CoA ligase
Location	Chr6: 27286024 .. 27288799 (-)
RNA-Seq Expression	CSPI06G32380
Synteny	CSPI06G32380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGAGTTTTATCCAAAGATGGATAAAGTAAAGAAAAGTAGATTTGATCCAAATATATTTCTCATACTCAGAAGGATCATATTTTGAAAGTGCCCATGTGCTTCAATCTACCGCCATCCCAATACCCATTTCTCAAATTGGCTGTTTCGTCTCTTCTCAAGAACCCAGATGAAGATTCTCTAAAATCCCATTGTAAAACTTCGCACGGATCAAAGAAAAACCCAAAAGGGGAGTTGTAGATTTCTTTCAACTTCTTCACAATCGCAACAATGAATCGGTTTCTTCACAACCACTTGTTCCGTTCAGTTGTGAGCTTGAACAGAGCGCTTTGGACTTGGAATTTCCCTTCGAACCGTCGTGCTTTCTCTGATTTGCCTGAATCTTGGAAATCGATGACGGGTTTGGTTCGATGCCCTGCAAACGATGTTCCTCTGTCTCCGATAAGCTTTTTGGAACGAACGGCTAAAGCTTACAGAGACACAACTTCGATTGTGTACGGTTCTATGTCGTTTACTTGGGAAGAAACCTATAATCGGTGTCTCAAATTGGCTTCTGCGATGACCCAGCTTGGGATTTCCTCTGGTCAAGTGGTGAGTTGCTCTAATTTGCATTGTTGATGTAGTGATTCAGTTGGGTTTCTTCACATTTTGTTTGGTGTTTTTGATTGTCATATTCTGATTCAATATGTATGTTGGTTGATTGTTGCAGAATGGATTAATGTTTTTTTTTTTTTTTTGAGTAGATACTGAATAGTGGGTAGGAATTGAAATCAACATTCTCCTGGATTTTGTGGCCAAATTCAAAGATTGTTCTATGCTAATTTTTGGGTTTAAATAGTGGTGGATGAGCTTGAGCTACTGGGACCATTTAAGATGTTATTAGAGCAGGTGGGCAATAGATGGTGTTAGATGATATAATATTAATTTTTTTTTTACATATCAGCTTTAAGTTTTTTTTTATCAATAAGTTATGTGAGAAATAGTAATTCAGTTTTCTTTAATGGTATGCTCTTTGATTCTGTTGTGGCTATGAGATTTCTTGCTGGAATTTGCAAGTTTGAACTTGTGTTGAACAGGTTGCAACTCTGGCTCCTAATGTCCCTGCCATGTATGAGCTCCACTTTGCTGTTCCCATGGCTGGTGCTGTTCTTTGTACTTTGAATAGTCGCCACGATTCATCTATGGTGTCAGTCTTGCTGAGACATTCAGAAGCTAAGATCATTTTTGTAGATCATCAGTTGTTTGAAGTTGCTTGCGAGGCAATCCGGCTTCTTGAACAAGGAGATTCAGAACCACCTAAACTAGTTTTGATTCTTGATTCTGAGCATGGTTCATCTCCAGCTTCGATATCTTCTAATGTTTATGAATATGAAAGTCTGATTGCATCTGGGAGTTGTGAATTTGAGGTCCGAAGACCGAAAAGCGAATGGGATCCAATTAGTATAAACTACACTTCTGGCACTACATCTATGCCTAAAGGGGTTGTATATAGTCATAGAGGTGCCTATCTCAATTCCTTGGCAACAGTTCTGCTCGGTGGAATGGGATCGATGCCGGTTTACCTTTGGACTTGTCCTATGTTTCATTGCAATGGATGGTGCCTTACTTGGGGAGTTGCAGCTCAAGGTGGTACAAATCTTTGTCTCAGAAAAGTCTCACCTAAGGCAATATTTGAGAAAATAGCTTTGCACAATGCCACACACATGGCTGCTGCACCCACTGTCTTGAACATGATTGCGAACTCTCCCGTGAGTGATCGCAGGCTACTTCCAAACAAGGTGGATGTCTTGACAGGTGGTTCGCCACCTCCGCCACAGGTACTTGCTGAGATGGAAGAGATGGGATTCCGAATCTGTCACTTGTATGGTCTAACTGAGACATATGGTCCTGGGACATATTGCACATGGAAGCCAATGTGGGATGATCTTCCTTCTGATGAAAAATCAAGGATTCGAGCTCGTCAAGGCATCCATCATGTTGGTCTGCAGGAGGTGGATGTAAGAGATCCCAAGACGATGGCTAGCGTTCCGACTGATGGGAAAACAATGGGAGAGATAATGTTTAGAGGGAATACTGTGATGAGTGGATACTTCAAAAACCAAAAAGCAACAGAGGAAGCTTTCAAGGGAGGGTGGTTTCACAGTGGAGATGCTGCAGTGAAACACCCTGATCATTATATCGAAGTAAAGGATCGCCTGAAGGACGTCATCATCTCAGGTGGGGAGAATATAAGCACAGTCGAGGTCGAAACTATTTTGTTCAGTCATCCATCAGTTCTAGAGGCTGCCGTTGTAGCCCGCCCTGATGATCATTGGGGAGAGACACCTTGTGCATTTGTCGTATTGAAGGAAGGATGCAACACCGTCACTGCCCAACAACTTATCGATTACTGTCGAGATCGTTTGCCGCATTACATGGCGCCTCGAACGATAGTATTTCAAGATTTGCCAAAGACTTCAACAGGAAAGGTTCAAAAGTTCATATTGAGGGAAAGAGCTAAGGCTATGGGAAGCCTTTCTTGAACTAATTTGGAGAAAAGAGATTCCAAATTATCTATTACACAGTACAAACCAATTATGATTCTTTAGACTAAACTATTGAGAGTTACTTTGTGATAATAGTTCTTGTTCAAATATAGAAATCAAACACACACACACACACATAAATAGGCATGCCTTTATTAAGGTGAGAAAAACAACAATTTATTCCAAAGTTACGGACATTACATCACATATTTCTGCGATCATACTTTGATGTTATGGTTTTTCTCACATATAT

mRNA sequence

GGGAGTTTTATCCAAAGATGGATAAAGTAAAGAAAAGTAGATTTGATCCAAATATATTTCTCATACTCAGAAGGATCATATTTTGAAAGTGCCCATGTGCTTCAATCTACCGCCATCCCAATACCCATTTCTCAAATTGGCTGTTTCGTCTCTTCTCAAGAACCCAGATGAAGATTCTCTAAAATCCCATTGTAAAACTTCGCACGGATCAAAGAAAAACCCAAAAGGGGAGTTGTAGATTTCTTTCAACTTCTTCACAATCGCAACAATGAATCGGTTTCTTCACAACCACTTGTTCCGTTCAGTTGTGAGCTTGAACAGAGCGCTTTGGACTTGGAATTTCCCTTCGAACCGTCGTGCTTTCTCTGATTTGCCTGAATCTTGGAAATCGATGACGGGTTTGGTTCGATGCCCTGCAAACGATGTTCCTCTGTCTCCGATAAGCTTTTTGGAACGAACGGCTAAAGCTTACAGAGACACAACTTCGATTGTGTACGGTTCTATGTCGTTTACTTGGGAAGAAACCTATAATCGGTGTCTCAAATTGGCTTCTGCGATGACCCAGCTTGGGATTTCCTCTGGTCAAGTGGTTGCAACTCTGGCTCCTAATGTCCCTGCCATGTATGAGCTCCACTTTGCTGTTCCCATGGCTGGTGCTGTTCTTTGTACTTTGAATAGTCGCCACGATTCATCTATGGTGTCAGTCTTGCTGAGACATTCAGAAGCTAAGATCATTTTTGTAGATCATCAGTTGTTTGAAGTTGCTTGCGAGGCAATCCGGCTTCTTGAACAAGGAGATTCAGAACCACCTAAACTAGTTTTGATTCTTGATTCTGAGCATGGTTCATCTCCAGCTTCGATATCTTCTAATGTTTATGAATATGAAAGTCTGATTGCATCTGGGAGTTGTGAATTTGAGGTCCGAAGACCGAAAAGCGAATGGGATCCAATTAGTATAAACTACACTTCTGGCACTACATCTATGCCTAAAGGGGTTGTATATAGTCATAGAGGTGCCTATCTCAATTCCTTGGCAACAGTTCTGCTCGGTGGAATGGGATCGATGCCGGTTTACCTTTGGACTTGTCCTATGTTTCATTGCAATGGATGGTGCCTTACTTGGGGAGTTGCAGCTCAAGGTGGTACAAATCTTTGTCTCAGAAAAGTCTCACCTAAGGCAATATTTGAGAAAATAGCTTTGCACAATGCCACACACATGGCTGCTGCACCCACTGTCTTGAACATGATTGCGAACTCTCCCGTGAGTGATCGCAGGCTACTTCCAAACAAGGTGGATGTCTTGACAGGTGGTTCGCCACCTCCGCCACAGGTACTTGCTGAGATGGAAGAGATGGGATTCCGAATCTGTCACTTGTATGGTCTAACTGAGACATATGGTCCTGGGACATATTGCACATGGAAGCCAATGTGGGATGATCTTCCTTCTGATGAAAAATCAAGGATTCGAGCTCGTCAAGGCATCCATCATGTTGGTCTGCAGGAGGTGGATGTAAGAGATCCCAAGACGATGGCTAGCGTTCCGACTGATGGGAAAACAATGGGAGAGATAATGTTTAGAGGGAATACTGTGATGAGTGGATACTTCAAAAACCAAAAAGCAACAGAGGAAGCTTTCAAGGGAGGGTGGTTTCACAGTGGAGATGCTGCAGTGAAACACCCTGATCATTATATCGAAGTAAAGGATCGCCTGAAGGACGTCATCATCTCAGGTGGGGAGAATATAAGCACAGTCGAGGTCGAAACTATTTTGTTCAGTCATCCATCAGTTCTAGAGGCTGCCGTTGTAGCCCGCCCTGATGATCATTGGGGAGAGACACCTTGTGCATTTGTCGTATTGAAGGAAGGATGCAACACCGTCACTGCCCAACAACTTATCGATTACTGTCGAGATCGTTTGCCGCATTACATGGCGCCTCGAACGATAGTATTTCAAGATTTGCCAAAGACTTCAACAGGAAAGGTTCAAAAGTTCATATTGAGGGAAAGAGCTAAGGCTATGGGAAGCCTTTCTTGAACTAATTTGGAGAAAAGAGATTCCAAATTATCTATTACACAGTACAAACCAATTATGATTCTTTAGACTAAACTATTGAGAGTTACTTTGTGATAATAGTTCTTGTTCAAATATAGAAATCAAACACACACACACACACATAAATAGGCATGCCTTTATTAAGGTGAGAAAAACAACAATTTATTCCAAAGTTACGGACATTACATCACATATTTCTGCGATCATACTTTGATGTTATGGTTTTTCTCACATATAT

Coding sequence (CDS)

ATGAATCGGTTTCTTCACAACCACTTGTTCCGTTCAGTTGTGAGCTTGAACAGAGCGCTTTGGACTTGGAATTTCCCTTCGAACCGTCGTGCTTTCTCTGATTTGCCTGAATCTTGGAAATCGATGACGGGTTTGGTTCGATGCCCTGCAAACGATGTTCCTCTGTCTCCGATAAGCTTTTTGGAACGAACGGCTAAAGCTTACAGAGACACAACTTCGATTGTGTACGGTTCTATGTCGTTTACTTGGGAAGAAACCTATAATCGGTGTCTCAAATTGGCTTCTGCGATGACCCAGCTTGGGATTTCCTCTGGTCAAGTGGTTGCAACTCTGGCTCCTAATGTCCCTGCCATGTATGAGCTCCACTTTGCTGTTCCCATGGCTGGTGCTGTTCTTTGTACTTTGAATAGTCGCCACGATTCATCTATGGTGTCAGTCTTGCTGAGACATTCAGAAGCTAAGATCATTTTTGTAGATCATCAGTTGTTTGAAGTTGCTTGCGAGGCAATCCGGCTTCTTGAACAAGGAGATTCAGAACCACCTAAACTAGTTTTGATTCTTGATTCTGAGCATGGTTCATCTCCAGCTTCGATATCTTCTAATGTTTATGAATATGAAAGTCTGATTGCATCTGGGAGTTGTGAATTTGAGGTCCGAAGACCGAAAAGCGAATGGGATCCAATTAGTATAAACTACACTTCTGGCACTACATCTATGCCTAAAGGGGTTGTATATAGTCATAGAGGTGCCTATCTCAATTCCTTGGCAACAGTTCTGCTCGGTGGAATGGGATCGATGCCGGTTTACCTTTGGACTTGTCCTATGTTTCATTGCAATGGATGGTGCCTTACTTGGGGAGTTGCAGCTCAAGGTGGTACAAATCTTTGTCTCAGAAAAGTCTCACCTAAGGCAATATTTGAGAAAATAGCTTTGCACAATGCCACACACATGGCTGCTGCACCCACTGTCTTGAACATGATTGCGAACTCTCCCGTGAGTGATCGCAGGCTACTTCCAAACAAGGTGGATGTCTTGACAGGTGGTTCGCCACCTCCGCCACAGGTACTTGCTGAGATGGAAGAGATGGGATTCCGAATCTGTCACTTGTATGGTCTAACTGAGACATATGGTCCTGGGACATATTGCACATGGAAGCCAATGTGGGATGATCTTCCTTCTGATGAAAAATCAAGGATTCGAGCTCGTCAAGGCATCCATCATGTTGGTCTGCAGGAGGTGGATGTAAGAGATCCCAAGACGATGGCTAGCGTTCCGACTGATGGGAAAACAATGGGAGAGATAATGTTTAGAGGGAATACTGTGATGAGTGGATACTTCAAAAACCAAAAAGCAACAGAGGAAGCTTTCAAGGGAGGGTGGTTTCACAGTGGAGATGCTGCAGTGAAACACCCTGATCATTATATCGAAGTAAAGGATCGCCTGAAGGACGTCATCATCTCAGGTGGGGAGAATATAAGCACAGTCGAGGTCGAAACTATTTTGTTCAGTCATCCATCAGTTCTAGAGGCTGCCGTTGTAGCCCGCCCTGATGATCATTGGGGAGAGACACCTTGTGCATTTGTCGTATTGAAGGAAGGATGCAACACCGTCACTGCCCAACAACTTATCGATTACTGTCGAGATCGTTTGCCGCATTACATGGCGCCTCGAACGATAGTATTTCAAGATTTGCCAAAGACTTCAACAGGAAAGGTTCAAAAGTTCATATTGAGGGAAAGAGCTAAGGCTATGGGAAGCCTTTCTTGA

Protein sequence

MNRFLHNHLFRSVVSLNRALWTWNFPSNRRAFSDLPESWKSMTGLVRCPANDVPLSPISFLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEPPKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS*

Homology

[Close]

BLAST of CSPI06G32380 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 1.5e-229
Identity = 386/603 (64.01%), Postives = 467/603 (77.45%), Query Frame = 0


Query: 1   MNRFLHNHLFRSVVSLNRALWTWNFP----SNRRAFSD--LPESWKSMTGLVRCPANDVP 60
           M   L    FR      + LW  + P    SN   F D   PESW+++ GL+R PAN  P
Sbjct: 1   MRFLLTKRAFRIFNPRFQRLWLTSSPFSSTSNSGGFPDDSEPESWRTIEGLLRSPANFSP 60

Query: 61  LSPISFLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPN 120
           LSPI+FLER+AK YRD TS+V+GS+  TW +TY RCL+LASA+T LGIS G VVA LAPN
Sbjct: 61  LSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLGISRGDVVAALAPN 120

Query: 121 VPAMYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLE 180
           VPAM+ELHFAVPMAG +LC LN+R D S +SVLL HSEAKI+FVDHQL E+A  A+ LL 
Sbjct: 121 VPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIAHGALDLLA 180

Query: 181 QGDSEPPKLVLIL----------DSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSE 240
           + D     L L+L          D +  S+ AS  S  YEYE+L+ SG  EFE+ +P+ E
Sbjct: 181 KSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEFEIIKPRCE 240

Query: 241 WDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLT 300
           WDPISINYTSGTTS PKGVVYSHRGAYLNSLATV L  M   PVYLWT PMFHCNGWCL 
Sbjct: 241 WDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLWTVPMFHCNGWCLV 300

Query: 301 WGVAAQGGTNLCLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDV 360
           WGVAAQGGTN+CLRKVSPK IF+ IA+H  THM  APTVLNMI N  V++ + LP++V++
Sbjct: 301 WGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEHKPLPHRVEI 360

Query: 361 LTGGSPPPPQVLAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQG 420
           +TGGSPP PQ+LA+MEE+GF + HLYGLTETYGPGT+C WKP WD L  +E+++++ARQG
Sbjct: 361 MTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEERTKLKARQG 420

Query: 421 IHHVGLQEVDVRDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSG 480
           + H+GL+ +DV+DP TM +VP DG TMGE+MFRGNTVMSGYFK+ +AT +AF+G WFHSG
Sbjct: 421 VQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRKAFEGDWFHSG 480

Query: 481 DAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETP 540
           D AVK+PD YIE+KDRLKDVIISGGENIS+VEVE +L SH +VLEAAVVARPD HWG+TP
Sbjct: 481 DLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAAVVARPDHHWGQTP 540

Query: 541 CAFVVLKEGCNTVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAM 588
           C FV LKEG +T+  +++I +CRD LPHYMAP+TIVF D+PKTSTGKVQK++LR++A  M
Sbjct: 541 CGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQKYLLRKKADEM 600

[Close]

BLAST of CSPI06G32380 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 1.3e-225
Identity = 378/555 (68.11%), Postives = 456/555 (82.16%), Query Frame = 0


Query: 38  SWKSMTGLVRCPANDVPLSPISFLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAM 97
           SWKSM GLV+C AN VPLSPI+FLER++KAYRD TS+VYGS+ +TW +T++RCLKLASA+
Sbjct: 25  SWKSMEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASAL 84

Query: 98  -TQLGISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKII 157
            T LGIS G VVAT + N+P +YELHFAVPMAG +LCTLN+R+DS+MVS LL HSEAK+I
Sbjct: 85  TTHLGISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLI 144

Query: 158 FVDHQLFEVACEAIRLLEQGDSEPPKLVLILDSEHGSSPASISSNVYE-YESLIASGSCE 217
           FV+ QL E A  A+ LL Q D +PP LVL+ DSE      S +S+ Y+ Y  L+A+GS +
Sbjct: 145 FVEPQLLETARAALDLLAQKDIKPPTLVLLTDSE------SFTSSSYDHYNHLLANGSDD 204

Query: 218 FEVRRPKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPM 277
           FE+RRPK+EWDPISINYTSGTT+ PK VVYSHRGAYLNS+ATVLL GMG+  VYLW+ PM
Sbjct: 205 FEIRRPKNEWDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTTSVYLWSVPM 264

Query: 278 FHCNGWCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDR 337
           FHCNGWC  WG AAQG TN+C+RKVSPKAIF+ I LH  TH  AAPTVLNMI NSP  + 
Sbjct: 265 FHCNGWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNL 324

Query: 338 RL-LPNKVDVLTGGSPPPPQVLAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSD 397
              LP+KV+V+TGGSPPPP+V+A MEEMGF++ H+YGLTET GP   C  KP WD L  +
Sbjct: 325 HTPLPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPE 384

Query: 398 EKSRIRARQGIHHVGLQEVDVRDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEE 457
           E+  ++ARQG++H+ ++E+DVRDP TM SV  DG T+GE+MFRGNTVMSGYFK+ KATEE
Sbjct: 385 ERYALKARQGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEE 444

Query: 458 AFKGGWFHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVA 517
           AF+GGWF SGD  VKH D YI++KDR KDV+ISGGENISTVEVET+L+SH +VLEAAVVA
Sbjct: 445 AFEGGWFRSGDLGVKHEDGYIQLKDRKKDVVISGGENISTVEVETVLYSHEAVLEAAVVA 504

Query: 518 RPDDHWGETPCAFVVLKEGC-NTVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQ 577
           RPD  WGETPCAFV LKEG  N V+A Q+I +CRDRLPHYMAP+T+VF++LPKTSTGK+Q
Sbjct: 505 RPDKLWGETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQ 564

Query: 578 KFILRERAKAMGSLS 589
           K+IL+E+A AMGSLS
Sbjct: 565 KYILKEKAMAMGSLS 573

[Close]

BLAST of CSPI06G32380 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 1.4e-224
Identity = 375/555 (67.57%), Postives = 457/555 (82.34%), Query Frame = 0


Query: 38  SWKSMTGLVRCPANDVPLSPISFLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAM 97
           SWKSM GLV+C AN VPLSPI+FLER++KAYRD TS+VYGS+ +TW +T++RCLKLASA+
Sbjct: 25  SWKSMEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASAL 84

Query: 98  -TQLGISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKII 157
            T  GIS G VVAT + N+P +YELHFAVPMAG +LCTLN+R+DS+MVS LL HSEAK+I
Sbjct: 85  TTHFGISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLI 144

Query: 158 FVDHQLFEVACEAIRLLEQGDSEPPKLVLILDSEHGSSPASISSNVYE-YESLIASGSCE 217
           FV+ QL E A  A+ LL Q D +PP LVL+ DSE      S +S+ Y+ Y  L+A+GS +
Sbjct: 145 FVEPQLLETARAALDLLAQKDIKPPTLVLLTDSE------SFTSSSYDHYNHLLANGSDD 204

Query: 218 FEVRRPKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPM 277
           FE+RRPK+E DPISINYTSGTT+ PK VVYSHRGAYLNS+ATVLL GMG+  VYLW+ PM
Sbjct: 205 FEIRRPKNECDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTRSVYLWSVPM 264

Query: 278 FHCNGWCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDR 337
           FHCNGWC  WG AAQG TN+C+RKVSPKAIF+ I LH  TH  AAPTVLNMI NSP  + 
Sbjct: 265 FHCNGWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNL 324

Query: 338 RL-LPNKVDVLTGGSPPPPQVLAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSD 397
              LP+KV+V+TGGSPPPP+V+A MEEMGF++ H+YGLTET+GP T C  KP WD L  +
Sbjct: 325 HTPLPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPE 384

Query: 398 EKSRIRARQGIHHVGLQEVDVRDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEE 457
           E+  ++ARQG++H+ ++E+DVRDP +M SV  DG T+GE+MFRGNTVMSGYFK+ KATEE
Sbjct: 385 ERYALKARQGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEE 444

Query: 458 AFKGGWFHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVA 517
           AF+GGWF +GD  VKH D YI++KDR KDV+ISGGEN+STVEVET+L+SH +VLEAAVVA
Sbjct: 445 AFEGGWFRTGDLGVKHEDGYIQLKDRKKDVVISGGENVSTVEVETVLYSHEAVLEAAVVA 504

Query: 518 RPDDHWGETPCAFVVLKEGC-NTVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQ 577
           RPD  WGETPCAFV LKEG  N V+A Q+I +CRDRLPHYMAP+T+VF++LPKTSTGK+Q
Sbjct: 505 RPDKLWGETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQ 564

Query: 578 KFILRERAKAMGSLS 589
           K+IL+E+AKAMGSLS
Sbjct: 565 KYILKEKAKAMGSLS 573

[Close]

BLAST of CSPI06G32380 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 3.0e-214
Identity = 352/548 (64.23%), Postives = 434/548 (79.20%), Query Frame = 0


Query: 42  MTGLVRCPANDVPLSPISFLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLG 101
           M G ++ PAN VPL+PISFL+R+A  Y D  SIVYGS+ +TW +T +RC+++ASA++QLG
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 102 ISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQ 161
           IS+G VV+ LAPNVPAM ELHF VPMAGA+LCTLN RHDSS+V+VLLRHS  K+IF DHQ
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 162 LFEVACEAIRLLEQGDSEPPKLVLILDS-EHGSSPASISSNVYEYESLIASGSCEFEVRR 221
             ++A  A  +L     + P LVLI +      S    S  + EYE ++A G  +FEV R
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 222 PKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNG 281
           P  E D IS+NYTSGTTS PKGVVYSHRGAYLNSLA VLL  M S P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 282 WCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPN 341
           WCL WGV A GGTN+CLR V+ KAIF+ I+ H  THM  APT+LNMI N+P S+++ LP 
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 342 KVDVLTGGSPPPPQVLAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIR 401
           KV  +TG +PPP  V+ +MEE+GF + H YGLTETYGPGT CTWKP WD LP +E+++++
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 402 ARQGIHHVGLQEVDVRDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGW 461
           ARQG++H+GL+E+ V+DP TM ++P DG TMGE++FRGNTVM+GY KN +AT+EAFKGGW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 462 FHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHW 521
           F SGD  VKHPD YIE+KDR KD+IISGGENIS++EVE+ LF+HP VLEAAVVARPD++W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 522 GETPCAFVVLKEGCNTVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRER 581
           GET CAFV LK+G +  +A++LI YCRDRLPHYMAPR+IVF+DLPKTSTGKVQKF+LR +
Sbjct: 483 GETACAFVKLKDG-SKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTK 542

Query: 582 AKAMGSLS 589
           AKA+ SLS
Sbjct: 543 AKALVSLS 549

[Close]

BLAST of CSPI06G32380 vs. ExPASy Swiss-Prot
Match: M4IS88 (Acetate--CoA ligase CCL3 OS=Humulus lupulus OX=3486 GN=CCL3 PE=1 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 7.7e-170
Identity = 286/558 (51.25%), Postives = 383/558 (68.64%), Query Frame = 0


Query: 40  KSMTGLVRCPANDVPLSPISFLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQ 99
           + +  L +  AN   L+P+ FLER A  +   TS+++GS  +TW +TY+RC + ASA+  
Sbjct: 6   RDIDDLPKNAANYTALTPLWFLERAATVHPTRTSVIHGSRHYTWLQTYHRCRQFASALNN 65

Query: 100 LGISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVD 159
             I  G  VA +APNVPA+YE HFAVPMAGAV+  +N R ++S ++ LL HS A  + VD
Sbjct: 66  HSIGLGSTVAVIAPNVPALYEAHFAVPMAGAVVNCVNIRLNASTIAFLLGHSSAAAVMVD 125

Query: 160 HQLFEVACEAIRLLEQGDS---EPPKLVLILDS--EHGSSPASISSNVYEYESLIASGSC 219
            + F +A EA+++L Q      +PP LV+I D   +  +   ++ +   EYE  +  G  
Sbjct: 126 QEFFSLAEEALKILAQESKSHYKPPLLVVIGDESCDPKTLEYALKTGAIEYEKFLEGGDP 185

Query: 220 EFEVRRPKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCP 279
           EF+ + P+ EW  IS+ YTSGTT+ PKGVV SHRGAYL SL+  ++ G+    +YLWT P
Sbjct: 186 EFDWKPPEDEWQSISLGYTSGTTASPKGVVLSHRGAYLMSLSASVVWGINEGAIYLWTLP 245

Query: 280 MFHCNGWCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSD 339
           MFHCNGWC TWG+AA  GTN+CLR+V+ K ++  IA +  TH  AAP VLN I N+P  +
Sbjct: 246 MFHCNGWCYTWGMAAFCGTNICLRQVTAKGVYSAIAKYGVTHFCAAPVVLNTIVNAPPEE 305

Query: 340 RRL-LPNKVDVLTGGSPPPPQVLAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPS 399
             + LP+ V V+T G+ PPP VL  M E GF++ H YGL+ETYGP T C WKP WD LP 
Sbjct: 306 AIIPLPHLVHVMTAGAAPPPSVLFAMSEKGFKVAHTYGLSETYGPSTICAWKPEWDSLPP 365

Query: 400 DEKSRIRARQGIHHVGLQEVDVRDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATE 459
            +++R+ ARQG+ ++ L+ +DV D KTM  VP DG TMGEI+ RGN VM GY KN KA E
Sbjct: 366 IKQARLNARQGVRYIALEGLDVVDTKTMKPVPADGTTMGEIVMRGNAVMKGYLKNPKANE 425

Query: 460 EAFKGGWFHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVV 519
           E+F  GWFHSGD AVKHPD YIE+KDR KD+IISGGENIS++EVE  L+ HP+VLE +VV
Sbjct: 426 ESFADGWFHSGDLAVKHPDGYIEIKDRSKDIIISGGENISSLEVENTLYLHPAVLEVSVV 485

Query: 520 ARPDDHWGETPCAFVVLKEGCN----TVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTST 579
           ARPD+ WGE+PCAFV LK   +     V A+ +I +C+ ++P Y  P+++VF  LPKT+T
Sbjct: 486 ARPDERWGESPCAFVTLKPNIDKSNEQVLAEDIIKFCKSKMPAYWVPKSVVFGPLPKTAT 545

Query: 580 GKVQKFILRERAKAMGSL 588
           GK+QK +LR +AK MG+L
Sbjct: 546 GKIQKHVLRAKAKEMGAL 563

[Close]

BLAST of CSPI06G32380 vs. ExPASy TrEMBL
Match: A0A0A0KGV7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G509050 PE=4 SV=1)

HSP 1 Score: 1208.7 bits (3126), Expect = 0.0e+00
Identity = 586/588 (99.66%), Postives = 586/588 (99.66%), Query Frame = 0


Query: 1   MNRFLHNHLFRSVVSLNRALWTWNFPSNRRAFSDLPESWKSMTGLVRCPANDVPLSPISF 60
           MNRFLHNHLFRSVVSLNRALWTWNFPSNRR FSDLPESWKSMTGLVRCPANDVPLSPISF
Sbjct: 1   MNRFLHNHLFRSVVSLNRALWTWNFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60

Query: 61  LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
           LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61  LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120

Query: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP 180
           LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP
Sbjct: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP 180

Query: 181 PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
           PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240

Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
           KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300

Query: 301 SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME 360
           SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME
Sbjct: 301 SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME 360

Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT 420
           EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT 420

Query: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480
           MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAF GGWFHSGDAAVKHPDHYIEVKDR
Sbjct: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFMGGWFHSGDAAVKHPDHYIEVKDR 480

Query: 481 LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
           LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ
Sbjct: 481 LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540

Query: 541 QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 589
           QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 588

[Close]

BLAST of CSPI06G32380 vs. ExPASy TrEMBL
Match: A0A1S3B1R0 (probable acyl-activating enzyme 1, peroxisomal isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485184 PE=4 SV=1)

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 574/588 (97.62%), Postives = 583/588 (99.15%), Query Frame = 0


Query: 1   MNRFLHNHLFRSVVSLNRALWTWNFPSNRRAFSDLPESWKSMTGLVRCPANDVPLSPISF 60
           MNRFLHNHLFRSV+SLNRAL TWNFPSNRR FSDLPESWKSMTGLVRCPANDVPLSPISF
Sbjct: 1   MNRFLHNHLFRSVLSLNRALSTWNFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60

Query: 61  LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
           LERTAKAYRDTTSIVYGS+SFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61  LERTAKAYRDTTSIVYGSLSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120

Query: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP 180
           LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAI+LLEQGDSEP
Sbjct: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIQLLEQGDSEP 180

Query: 181 PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
           PKLVLILDSEHGSSPAS+SSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240

Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
           KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWG+AAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGIAAQGGTNLCLRKV 300

Query: 301 SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME 360
           SPKAIFE IALHNATHMAAAPTVLNMI NSPVSDRRLLPNKVDVLTGGSPPPPQV AEME
Sbjct: 301 SPKAIFETIALHNATHMAAAPTVLNMIVNSPVSDRRLLPNKVDVLTGGSPPPPQVFAEME 360

Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT 420
           EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQG+HHVGLQEVDVRDP+T
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGVHHVGLQEVDVRDPET 420

Query: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480
           MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR
Sbjct: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480

Query: 481 LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
           LKDVIISGGENISTVEVET+LFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ
Sbjct: 481 LKDVIISGGENISTVEVETVLFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540

Query: 541 QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 589
           QLIDYCRDRLPHYMAPR+IVFQDLPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRSIVFQDLPKTSTGKVQKFILRERAKAMGSLS 588

[Close]

BLAST of CSPI06G32380 vs. ExPASy TrEMBL
Match: A0A5A7SME5 (Putative acyl-activating enzyme 1, peroxisomal isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00680 PE=4 SV=1)

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 573/588 (97.45%), Postives = 582/588 (98.98%), Query Frame = 0


Query: 1   MNRFLHNHLFRSVVSLNRALWTWNFPSNRRAFSDLPESWKSMTGLVRCPANDVPLSPISF 60
           MNRFLHNHLFRSV+SLNRAL TWNFPSNRR FSDLPESWKSMTGLVRCPANDVPLSPISF
Sbjct: 1   MNRFLHNHLFRSVLSLNRALSTWNFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60

Query: 61  LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
           LERTAKAYRDTTSIVYGS+SFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61  LERTAKAYRDTTSIVYGSLSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120

Query: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP 180
           LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAI+LLEQGDSEP
Sbjct: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIQLLEQGDSEP 180

Query: 181 PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
           PKLVLILDSEHGSSPAS+SSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240

Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
           KGVVYSHRGAYLNSLATVLLGGM SMPVYLWTCPMFHCNGWCLTWG+AAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMRSMPVYLWTCPMFHCNGWCLTWGIAAQGGTNLCLRKV 300

Query: 301 SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME 360
           SPKAIFE IALHNATHMAAAPTVLNMI NSPVSDRRLLPNKVDVLTGGSPPPPQV AEME
Sbjct: 301 SPKAIFETIALHNATHMAAAPTVLNMIVNSPVSDRRLLPNKVDVLTGGSPPPPQVFAEME 360

Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT 420
           EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQG+HHVGLQEVDVRDP+T
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGVHHVGLQEVDVRDPET 420

Query: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480
           MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR
Sbjct: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480

Query: 481 LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
           LKDVIISGGENISTVEVET+LFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ
Sbjct: 481 LKDVIISGGENISTVEVETVLFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540

Query: 541 QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 589
           QLIDYCRDRLPHYMAPR+IVFQDLPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRSIVFQDLPKTSTGKVQKFILRERAKAMGSLS 588

[Close]

BLAST of CSPI06G32380 vs. ExPASy TrEMBL
Match: A0A6J1BTA3 (probable acyl-activating enzyme 1, peroxisomal isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005564 PE=4 SV=1)

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 524/593 (88.36%), Postives = 558/593 (94.10%), Query Frame = 0


Query: 1   MNRFLHNHLFRSVVSLNRALW--TWN--FPSNRRAFSDLPESWKSMTGLVRCPANDVPLS 60
           MNRFL N L RSV S NR LW   WN  FPS+RR F+++P+SWKSM GLVRCPANDVPLS
Sbjct: 1   MNRFLQNQLLRSVCSFNRPLWNRNWNLDFPSSRRGFANVPDSWKSMAGLVRCPANDVPLS 60

Query: 61  PISFLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVP 120
           P+SFLERTAK YRDTTS+VYGS+SFTWEETYNRCLKLASA++QLGIS GQVVATLAPNVP
Sbjct: 61  PLSFLERTAKVYRDTTSVVYGSVSFTWEETYNRCLKLASAISQLGISPGQVVATLAPNVP 120

Query: 121 AMYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQG 180
           AMYELHFAVPMAGAVLCTLN+RHDSSMVS LLRHSEAKIIFVD QLFEVACEAI+LL QG
Sbjct: 121 AMYELHFAVPMAGAVLCTLNARHDSSMVSALLRHSEAKIIFVDFQLFEVACEAIQLLAQG 180

Query: 181 DSEPPKLVLILDSEHGSSPAS-ISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSG 240
           DSE PKLVLILD+EHG SPA+ +SSNV EYESLIASGSCEFEV++PKSEWDPISINYTSG
Sbjct: 181 DSESPKLVLILDAEHGLSPANPLSSNVCEYESLIASGSCEFEVQKPKSEWDPISINYTSG 240

Query: 241 TTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNL 300
           TTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCL WGVAAQGGTN+
Sbjct: 241 TTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLAWGVAAQGGTNI 300

Query: 301 CLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQV 360
           CLRKVSPKAIFEKIAL N THMAAAPTVLNMI NSPVSDRRLLPNKVDVLTGG+PPPPQ+
Sbjct: 301 CLRKVSPKAIFEKIALQNVTHMAAAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQI 360

Query: 361 LAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDV 420
           LA+MEEMGFRICHLYGLTETYGPGTYCTWKPMWD LPSDE++RIRARQG+HHVGLQEVDV
Sbjct: 361 LAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDSLPSDERARIRARQGVHHVGLQEVDV 420

Query: 421 RDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYI 480
           RDP TMA VP DGKTMGEIMFRGNTVMSGYFKNQKATEEAF+GGWFHSGDAAVKHPDHYI
Sbjct: 421 RDPVTMAGVPADGKTMGEIMFRGNTVMSGYFKNQKATEEAFRGGWFHSGDAAVKHPDHYI 480

Query: 481 EVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCN 540
           EVKDRLKD++ISGGENISTVEVETILF HP+VLEAAVV RPD+HWGETPCAFV LKEGCN
Sbjct: 481 EVKDRLKDIVISGGENISTVEVETILFGHPAVLEAAVVGRPDNHWGETPCAFVTLKEGCN 540

Query: 541 TVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 589
            +TAQQLIDYCRD LPHYMAPR+I+FQDLPKTSTGK+QKFILR+RAKAMGSLS
Sbjct: 541 -ITAQQLIDYCRDHLPHYMAPRSIIFQDLPKTSTGKIQKFILRDRAKAMGSLS 592

[Close]

BLAST of CSPI06G32380 vs. ExPASy TrEMBL
Match: A0A6J1KUK0 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111497331 PE=4 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 5.2e-302
Identity = 500/589 (84.89%), Postives = 537/589 (91.17%), Query Frame = 0


Query: 1   MNRFLHNHLFRSVVSLNRALWTWNFPSN-RRAFSDLPESWKSMTGLVRCPANDVPLSPIS 60
           MNRF+ N  FRSV SLNRA+W W+FPS   R FSDL +SWKS  GLVRCPANDVPLSP++
Sbjct: 1   MNRFIRNRSFRSVFSLNRAVWKWDFPSTICRGFSDLSDSWKSTAGLVRCPANDVPLSPVT 60

Query: 61  FLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMY 120
           FLERTAK YRDT S+VYGS+SFTWEETYNRCL+LASAMTQLGIS GQVVATLAPN+PAMY
Sbjct: 61  FLERTAKVYRDTASVVYGSVSFTWEETYNRCLRLASAMTQLGISPGQVVATLAPNIPAMY 120

Query: 121 ELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSE 180
           ELHFA PMAGAVLCTLN+RHDSSM+SVLLRHSEAKIIFVD QLFEVA EAI+LL QGDS+
Sbjct: 121 ELHFATPMAGAVLCTLNTRHDSSMLSVLLRHSEAKIIFVDTQLFEVANEAIQLLRQGDSQ 180

Query: 181 PPKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSM 240
           PPKLVLI DSE+ SSP S+SSN YEYE+LIASGSCEFEVR P+SEWDPISINYTSGTTSM
Sbjct: 181 PPKLVLIHDSEYASSPTSLSSNAYEYETLIASGSCEFEVRNPRSEWDPISINYTSGTTSM 240

Query: 241 PKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRK 300
           PKGVVYSHRGAYLNSLATVLL GMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTN+CLRK
Sbjct: 241 PKGVVYSHRGAYLNSLATVLLAGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNICLRK 300

Query: 301 VSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEM 360
           VSPKAIFE+IALH  THMAAAPTVLNMI NSP  DRR LPN V VLTGGSPPPP V  ++
Sbjct: 301 VSPKAIFEQIALHKVTHMAAAPTVLNMIVNSPECDRRPLPNTVVVLTGGSPPPPHVFTKV 360

Query: 361 EEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPK 420
           EEMGF+ICHLYGLTETYGPGTYCTWKPMWD LP DE+SRIR RQG+ HVGL +V+V DPK
Sbjct: 361 EEMGFQICHLYGLTETYGPGTYCTWKPMWDCLPCDERSRIRVRQGVRHVGLHDVNVIDPK 420

Query: 421 TMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKD 480
           TM  VP DGKT+GEIMFRGNTVMSGYFKN+KATEEAFKGGWFHSGDA VKHP+HYIEVKD
Sbjct: 421 TMVGVPADGKTVGEIMFRGNTVMSGYFKNKKATEEAFKGGWFHSGDAGVKHPNHYIEVKD 480

Query: 481 RLKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTA 540
           RLKDVIISGGENIS+VEVE +LF HP++LEAAVV RPDDHWGETPCAFV LKEGCN VTA
Sbjct: 481 RLKDVIISGGENISSVEVEAVLFGHPAILEAAVVGRPDDHWGETPCAFVALKEGCN-VTA 540

Query: 541 QQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 589
           QQLI+YCR RLPHYMAPR+IVFQDLPKTSTGKVQKFILR+RAKAMGSLS
Sbjct: 541 QQLIEYCRHRLPHYMAPRSIVFQDLPKTSTGKVQKFILRDRAKAMGSLS 588

[Close]

BLAST of CSPI06G32380 vs. NCBI nr
Match: XP_004134830.2 (probable acyl-activating enzyme 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 1208.7 bits (3126), Expect = 0.0e+00
Identity = 586/588 (99.66%), Postives = 586/588 (99.66%), Query Frame = 0


Query: 1   MNRFLHNHLFRSVVSLNRALWTWNFPSNRRAFSDLPESWKSMTGLVRCPANDVPLSPISF 60
           MNRFLHNHLFRSVVSLNRALWTWNFPSNRR FSDLPESWKSMTGLVRCPANDVPLSPISF
Sbjct: 1   MNRFLHNHLFRSVVSLNRALWTWNFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60

Query: 61  LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
           LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61  LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120

Query: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP 180
           LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP
Sbjct: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP 180

Query: 181 PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
           PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240

Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
           KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300

Query: 301 SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME 360
           SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME
Sbjct: 301 SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME 360

Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT 420
           EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT 420

Query: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480
           MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAF GGWFHSGDAAVKHPDHYIEVKDR
Sbjct: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFMGGWFHSGDAAVKHPDHYIEVKDR 480

Query: 481 LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
           LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ
Sbjct: 481 LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540

Query: 541 QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 589
           QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 588

[Close]

BLAST of CSPI06G32380 vs. NCBI nr
Match: XP_008440901.1 (PREDICTED: probable acyl-activating enzyme 1, peroxisomal isoform X1 [Cucumis melo])

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 574/588 (97.62%), Postives = 583/588 (99.15%), Query Frame = 0


Query: 1   MNRFLHNHLFRSVVSLNRALWTWNFPSNRRAFSDLPESWKSMTGLVRCPANDVPLSPISF 60
           MNRFLHNHLFRSV+SLNRAL TWNFPSNRR FSDLPESWKSMTGLVRCPANDVPLSPISF
Sbjct: 1   MNRFLHNHLFRSVLSLNRALSTWNFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60

Query: 61  LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
           LERTAKAYRDTTSIVYGS+SFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61  LERTAKAYRDTTSIVYGSLSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120

Query: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP 180
           LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAI+LLEQGDSEP
Sbjct: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIQLLEQGDSEP 180

Query: 181 PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
           PKLVLILDSEHGSSPAS+SSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240

Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
           KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWG+AAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGIAAQGGTNLCLRKV 300

Query: 301 SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME 360
           SPKAIFE IALHNATHMAAAPTVLNMI NSPVSDRRLLPNKVDVLTGGSPPPPQV AEME
Sbjct: 301 SPKAIFETIALHNATHMAAAPTVLNMIVNSPVSDRRLLPNKVDVLTGGSPPPPQVFAEME 360

Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT 420
           EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQG+HHVGLQEVDVRDP+T
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGVHHVGLQEVDVRDPET 420

Query: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480
           MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR
Sbjct: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480

Query: 481 LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
           LKDVIISGGENISTVEVET+LFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ
Sbjct: 481 LKDVIISGGENISTVEVETVLFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540

Query: 541 QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 589
           QLIDYCRDRLPHYMAPR+IVFQDLPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRSIVFQDLPKTSTGKVQKFILRERAKAMGSLS 588

[Close]

BLAST of CSPI06G32380 vs. NCBI nr
Match: KAA0025615.1 (putative acyl-activating enzyme 1, peroxisomal isoform X1 [Cucumis melo var. makuwa] >TYK12490.1 putative acyl-activating enzyme 1, peroxisomal isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 573/588 (97.45%), Postives = 582/588 (98.98%), Query Frame = 0


Query: 1   MNRFLHNHLFRSVVSLNRALWTWNFPSNRRAFSDLPESWKSMTGLVRCPANDVPLSPISF 60
           MNRFLHNHLFRSV+SLNRAL TWNFPSNRR FSDLPESWKSMTGLVRCPANDVPLSPISF
Sbjct: 1   MNRFLHNHLFRSVLSLNRALSTWNFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60

Query: 61  LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
           LERTAKAYRDTTSIVYGS+SFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61  LERTAKAYRDTTSIVYGSLSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120

Query: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP 180
           LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAI+LLEQGDSEP
Sbjct: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIQLLEQGDSEP 180

Query: 181 PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
           PKLVLILDSEHGSSPAS+SSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240

Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
           KGVVYSHRGAYLNSLATVLLGGM SMPVYLWTCPMFHCNGWCLTWG+AAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMRSMPVYLWTCPMFHCNGWCLTWGIAAQGGTNLCLRKV 300

Query: 301 SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME 360
           SPKAIFE IALHNATHMAAAPTVLNMI NSPVSDRRLLPNKVDVLTGGSPPPPQV AEME
Sbjct: 301 SPKAIFETIALHNATHMAAAPTVLNMIVNSPVSDRRLLPNKVDVLTGGSPPPPQVFAEME 360

Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT 420
           EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQG+HHVGLQEVDVRDP+T
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGVHHVGLQEVDVRDPET 420

Query: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480
           MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR
Sbjct: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480

Query: 481 LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
           LKDVIISGGENISTVEVET+LFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ
Sbjct: 481 LKDVIISGGENISTVEVETVLFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540

Query: 541 QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 589
           QLIDYCRDRLPHYMAPR+IVFQDLPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRSIVFQDLPKTSTGKVQKFILRERAKAMGSLS 588

[Close]

BLAST of CSPI06G32380 vs. NCBI nr
Match: XP_038882615.1 (probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida])

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 551/588 (93.71%), Postives = 569/588 (96.77%), Query Frame = 0


Query: 1   MNRFLHNHLFRSVVSLNRALWTWNFPSNRRAFSDLPESWKSMTGLVRCPANDVPLSPISF 60
           MNRFLHNHLFRSV+SLNRALWT NFPSNRR FSDLPESWKSM GLVRCPANDVPLSPISF
Sbjct: 1   MNRFLHNHLFRSVLSLNRALWTRNFPSNRRGFSDLPESWKSMMGLVRCPANDVPLSPISF 60

Query: 61  LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
           LERTAK YRDT SIVYGSMSFTWEETY+RCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61  LERTAKVYRDTPSIVYGSMSFTWEETYDRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120

Query: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP 180
           LHFAVPM GA+LCTLN+RHDSSMVSVLLRHSEAKIIFVD+QLFEVACEAI+LLE+GDSEP
Sbjct: 121 LHFAVPMVGAILCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLERGDSEP 180

Query: 181 PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
           PKLVLILDSEHGSSPAS+SSNVYEYESLIASGSC+FEVR+PKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCDFEVRKPKSEWDPISINYTSGTTSMP 240

Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
           KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300

Query: 301 SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME 360
            PK IFE IALHN THMA APTVLNMI NS VSDRRLLPNKVDVLTGGSPPPPQVLA+ME
Sbjct: 301 LPKTIFENIALHNVTHMAGAPTVLNMIVNSQVSDRRLLPNKVDVLTGGSPPPPQVLAKME 360

Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT 420
           EMGFRICHLYGLTETYGPGTYCTWKPMWD LPS+E+SRIRARQG+HHVGL+EVDVRDP T
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDGLPSNERSRIRARQGVHHVGLEEVDVRDPVT 420

Query: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480
           MASVP DGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKH DHYIEVKDR
Sbjct: 421 MASVPADGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHLDHYIEVKDR 480

Query: 481 LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
           LKDVIISGGENISTVEVET+LF HPSVLEAAVVARPDDHWGETPCAFVVLKEGCN VTAQ
Sbjct: 481 LKDVIISGGENISTVEVETVLFGHPSVLEAAVVARPDDHWGETPCAFVVLKEGCN-VTAQ 540

Query: 541 QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 589
           QLIDYCRDRLPHYMAPR+I+FQDLPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRSIIFQDLPKTSTGKVQKFILRERAKAMGSLS 587

[Close]

BLAST of CSPI06G32380 vs. NCBI nr
Match: XP_022132791.1 (probable acyl-activating enzyme 1, peroxisomal isoform X1 [Momordica charantia])

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 524/593 (88.36%), Postives = 558/593 (94.10%), Query Frame = 0


Query: 1   MNRFLHNHLFRSVVSLNRALW--TWN--FPSNRRAFSDLPESWKSMTGLVRCPANDVPLS 60
           MNRFL N L RSV S NR LW   WN  FPS+RR F+++P+SWKSM GLVRCPANDVPLS
Sbjct: 1   MNRFLQNQLLRSVCSFNRPLWNRNWNLDFPSSRRGFANVPDSWKSMAGLVRCPANDVPLS 60

Query: 61  PISFLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVP 120
           P+SFLERTAK YRDTTS+VYGS+SFTWEETYNRCLKLASA++QLGIS GQVVATLAPNVP
Sbjct: 61  PLSFLERTAKVYRDTTSVVYGSVSFTWEETYNRCLKLASAISQLGISPGQVVATLAPNVP 120

Query: 121 AMYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQG 180
           AMYELHFAVPMAGAVLCTLN+RHDSSMVS LLRHSEAKIIFVD QLFEVACEAI+LL QG
Sbjct: 121 AMYELHFAVPMAGAVLCTLNARHDSSMVSALLRHSEAKIIFVDFQLFEVACEAIQLLAQG 180

Query: 181 DSEPPKLVLILDSEHGSSPAS-ISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSG 240
           DSE PKLVLILD+EHG SPA+ +SSNV EYESLIASGSCEFEV++PKSEWDPISINYTSG
Sbjct: 181 DSESPKLVLILDAEHGLSPANPLSSNVCEYESLIASGSCEFEVQKPKSEWDPISINYTSG 240

Query: 241 TTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNL 300
           TTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCL WGVAAQGGTN+
Sbjct: 241 TTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLAWGVAAQGGTNI 300

Query: 301 CLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQV 360
           CLRKVSPKAIFEKIAL N THMAAAPTVLNMI NSPVSDRRLLPNKVDVLTGG+PPPPQ+
Sbjct: 301 CLRKVSPKAIFEKIALQNVTHMAAAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQI 360

Query: 361 LAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDV 420
           LA+MEEMGFRICHLYGLTETYGPGTYCTWKPMWD LPSDE++RIRARQG+HHVGLQEVDV
Sbjct: 361 LAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDSLPSDERARIRARQGVHHVGLQEVDV 420

Query: 421 RDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYI 480
           RDP TMA VP DGKTMGEIMFRGNTVMSGYFKNQKATEEAF+GGWFHSGDAAVKHPDHYI
Sbjct: 421 RDPVTMAGVPADGKTMGEIMFRGNTVMSGYFKNQKATEEAFRGGWFHSGDAAVKHPDHYI 480

Query: 481 EVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCN 540
           EVKDRLKD++ISGGENISTVEVETILF HP+VLEAAVV RPD+HWGETPCAFV LKEGCN
Sbjct: 481 EVKDRLKDIVISGGENISTVEVETILFGHPAVLEAAVVGRPDNHWGETPCAFVTLKEGCN 540

Query: 541 TVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 589
            +TAQQLIDYCRD LPHYMAPR+I+FQDLPKTSTGK+QKFILR+RAKAMGSLS
Sbjct: 541 -ITAQQLIDYCRDHLPHYMAPRSIIFQDLPKTSTGKIQKFILRDRAKAMGSLS 592

[Close]

BLAST of CSPI06G32380 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 797.0 bits (2057), Expect = 1.0e-230
Identity = 386/603 (64.01%), Postives = 467/603 (77.45%), Query Frame = 0


Query: 1   MNRFLHNHLFRSVVSLNRALWTWNFP----SNRRAFSD--LPESWKSMTGLVRCPANDVP 60
           M   L    FR      + LW  + P    SN   F D   PESW+++ GL+R PAN  P
Sbjct: 1   MRFLLTKRAFRIFNPRFQRLWLTSSPFSSTSNSGGFPDDSEPESWRTIEGLLRSPANFSP 60

Query: 61  LSPISFLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPN 120
           LSPI+FLER+AK YRD TS+V+GS+  TW +TY RCL+LASA+T LGIS G VVA LAPN
Sbjct: 61  LSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLGISRGDVVAALAPN 120

Query: 121 VPAMYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLE 180
           VPAM+ELHFAVPMAG +LC LN+R D S +SVLL HSEAKI+FVDHQL E+A  A+ LL 
Sbjct: 121 VPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIAHGALDLLA 180

Query: 181 QGDSEPPKLVLIL----------DSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSE 240
           + D     L L+L          D +  S+ AS  S  YEYE+L+ SG  EFE+ +P+ E
Sbjct: 181 KSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEFEIIKPRCE 240

Query: 241 WDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLT 300
           WDPISINYTSGTTS PKGVVYSHRGAYLNSLATV L  M   PVYLWT PMFHCNGWCL 
Sbjct: 241 WDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLWTVPMFHCNGWCLV 300

Query: 301 WGVAAQGGTNLCLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDV 360
           WGVAAQGGTN+CLRKVSPK IF+ IA+H  THM  APTVLNMI N  V++ + LP++V++
Sbjct: 301 WGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEHKPLPHRVEI 360

Query: 361 LTGGSPPPPQVLAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQG 420
           +TGGSPP PQ+LA+MEE+GF + HLYGLTETYGPGT+C WKP WD L  +E+++++ARQG
Sbjct: 361 MTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEERTKLKARQG 420

Query: 421 IHHVGLQEVDVRDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSG 480
           + H+GL+ +DV+DP TM +VP DG TMGE+MFRGNTVMSGYFK+ +AT +AF+G WFHSG
Sbjct: 421 VQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRKAFEGDWFHSG 480

Query: 481 DAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETP 540
           D AVK+PD YIE+KDRLKDVIISGGENIS+VEVE +L SH +VLEAAVVARPD HWG+TP
Sbjct: 481 DLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAAVVARPDHHWGQTP 540

Query: 541 CAFVVLKEGCNTVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAM 588
           C FV LKEG +T+  +++I +CRD LPHYMAP+TIVF D+PKTSTGKVQK++LR++A  M
Sbjct: 541 CGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQKYLLRKKADEM 600

[Close]

BLAST of CSPI06G32380 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 746.1 bits (1925), Expect = 2.1e-215
Identity = 352/548 (64.23%), Postives = 434/548 (79.20%), Query Frame = 0


Query: 42  MTGLVRCPANDVPLSPISFLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLG 101
           M G ++ PAN VPL+PISFL+R+A  Y D  SIVYGS+ +TW +T +RC+++ASA++QLG
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 102 ISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQ 161
           IS+G VV+ LAPNVPAM ELHF VPMAGA+LCTLN RHDSS+V+VLLRHS  K+IF DHQ
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 162 LFEVACEAIRLLEQGDSEPPKLVLILDS-EHGSSPASISSNVYEYESLIASGSCEFEVRR 221
             ++A  A  +L     + P LVLI +      S    S  + EYE ++A G  +FEV R
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 222 PKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNG 281
           P  E D IS+NYTSGTTS PKGVVYSHRGAYLNSLA VLL  M S P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 282 WCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPN 341
           WCL WGV A GGTN+CLR V+ KAIF+ I+ H  THM  APT+LNMI N+P S+++ LP 
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 342 KVDVLTGGSPPPPQVLAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIR 401
           KV  +TG +PPP  V+ +MEE+GF + H YGLTETYGPGT CTWKP WD LP +E+++++
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 402 ARQGIHHVGLQEVDVRDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGW 461
           ARQG++H+GL+E+ V+DP TM ++P DG TMGE++FRGNTVM+GY KN +AT+EAFKGGW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 462 FHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHW 521
           F SGD  VKHPD YIE+KDR KD+IISGGENIS++EVE+ LF+HP VLEAAVVARPD++W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 522 GETPCAFVVLKEGCNTVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRER 581
           GET CAFV LK+G +  +A++LI YCRDRLPHYMAPR+IVF+DLPKTSTGKVQKF+LR +
Sbjct: 483 GETACAFVKLKDG-SKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTK 542

Query: 582 AKAMGSLS 589
           AKA+ SLS
Sbjct: 543 AKALVSLS 549

[Close]

BLAST of CSPI06G32380 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 648.7 bits (1672), Expect = 4.6e-186
Identity = 306/472 (64.83%), Postives = 372/472 (78.81%), Query Frame = 0


Query: 118 MYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGD 177
           M ELHF VPMAGA+LCTLN RHDSS+V+VLLRHS  K+IF DHQ  ++A  A  +L    
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60

Query: 178 SEPPKLVLILDS-EHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGT 237
            + P LVLI +      S    S  + EYE ++A G  +FEV RP  E D IS+NYTSGT
Sbjct: 61  DKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTSGT 120

Query: 238 TSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLC 297
           TS PKGVVYSHRGAYLNSLA VLL  M S P YLWT PMFHCNGWCL WGV A GGTN+C
Sbjct: 121 TSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNGWCLLWGVTAIGGTNIC 180

Query: 298 LRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVL 357
           LR V+ KAIF+ I+ H  THM  APT+LNMI N+P S+++ LP KV  +TG +PPP  V+
Sbjct: 181 LRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPPPAHVI 240

Query: 358 AEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVR 417
            +MEE+GF + H YGLTETYGPGT CTWKP WD LP +E+++++ARQG++H+GL+E+ V+
Sbjct: 241 FKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLEEIQVK 300

Query: 418 DPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIE 477
           DP TM ++P DG TMGE++FRGNTVM+GY KN +AT+EAFKGGWF SGD  VKHPD YIE
Sbjct: 301 DPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHPDGYIE 360

Query: 478 VKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNT 537
           +KDR KD+IISGGENIS++EVE+ LF+HP VLEAAVVARPD++WGET CAFV LK+G + 
Sbjct: 361 LKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDG-SK 420

Query: 538 VTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 589
            +A++LI YCRDRLPHYMAPR+IVF+DLPKTSTGKVQKF+LR +AKA+ SLS
Sbjct: 421 ASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKALVSLS 471

[Close]

BLAST of CSPI06G32380 vs. TAIR 10
Match: AT3G16910.1 (acyl-activating enzyme 7 )

HSP 1 Score: 597.4 bits (1539), Expect = 1.2e-170
Identity = 281/557 (50.45%), Postives = 380/557 (68.22%), Query Frame = 0


Query: 39  WKSMTGLVRCPANDVPLSPISFLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMT 98
           W+ +  L + PAN   L+P+ FL+R A  +    S+++GS  +TW +TY+RC +LASA+ 
Sbjct: 6   WRDIDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALA 65

Query: 99  QLGISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFV 158
              I  G  VA +APN+PAMYE HF VPM GAVL  +N R ++  V+ LL HS++ +I V
Sbjct: 66  DRSIGPGSTVAIIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMV 125

Query: 159 DHQLFEVACEAIRLLEQ-GDSEPPKLVLILDSEHGSSPAS----ISSNVYEYESLIASGS 218
           D + F +A +++RL+E+   S   + +LI+  +H  +P S    +S    EYE  +A+G 
Sbjct: 126 DQEFFTLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGD 185

Query: 219 CEFEVRRPKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTC 278
             +  + P  EW  I++ YTSGTT+ PKGVV  HRGAY+ +L+  L+ GM    VYLWT 
Sbjct: 186 PNYPWQPPADEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTL 245

Query: 279 PMFHCNGWCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVS 338
           PMFHCNGWC  W +A   GT++CLR+V+ K ++  IA +  TH  AAP VLN I N+P  
Sbjct: 246 PMFHCNGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKE 305

Query: 339 DRRL-LPNKVDVLTGGSPPPPQVLAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLP 398
           D  L LP+ V V+T G+ PPP VL  M + GFR+ H YGL+ETYGP T C WKP WD LP
Sbjct: 306 DTILPLPHTVHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLP 365

Query: 399 SDEKSRIRARQGIHHVGLQEVDVRDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKAT 458
            + ++++ ARQG+ + G++++DV D +T   VP DGKT GEI+FRGN VM GY KN +A 
Sbjct: 366 PETQAKLNARQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEAN 425

Query: 459 EEAFKGGWFHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAV 518
           +E F GGWFHSGD AVKHPD+YIE+KDR KDVIISGGENIS+VEVE +++ HP+VLEA+V
Sbjct: 426 KETFAGGWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASV 485

Query: 519 VARPDDHWGETPCAFVVLKEGC----NTVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTS 578
           VARPD+ W E+PCAFV LK           AQ ++ +CR++LP Y  P+++VF  LPKT+
Sbjct: 486 VARPDERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTA 545

Query: 579 TGKVQKFILRERAKAMG 586
           TGK+QK ILR +AK MG
Sbjct: 546 TGKIQKHILRTKAKEMG 562

[Close]

BLAST of CSPI06G32380 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 575.1 bits (1481), Expect = 6.5e-164
Identity = 276/551 (50.09%), Postives = 386/551 (70.05%), Query Frame = 0


Query: 42  MTGLVRCPANDVPLSPISFLERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLG 101
           M  LV C AN+VPL+PI+FL+R ++ Y + TSI+YG   FTW +TY+RC +LA+++  L 
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 102 ISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQ 161
           I+   VV+ LAPNVPAMYE+HF+VPM GAVL  +N+R D+  ++++LRH+E KI+FVD++
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 162 LFEVACEAIRLLEQGDSEP-PKLVLILDSEHGSSPASISSNVYEYESLIASG----SCEF 221
              +  E +RL+    S+P P+++LI + +  + P    S   +YE LI  G    S   
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKP---FSKELDYEGLIRKGEPTPSSSA 180

Query: 222 EVRRPKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMF 281
            + R  +E DPIS+NYTSGTT+ PKGVV SH+GAYL++L++++   MG  PVYLWT PMF
Sbjct: 181 SMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMF 240

Query: 282 HCNGWCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRR 341
           HCNGW  TW VAA+GGTN+C+R V+   I++ I LH  THM+  PTV   +     +D+ 
Sbjct: 241 HCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQS 300

Query: 342 LLPNKVDVLTGGSPPPPQVLAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEK 401
              + V VLTGGS PP  ++ ++E++GF + H YGLTE  GP  +C W+  W+ LP  ++
Sbjct: 301 PKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQ 360

Query: 402 SRIRARQGIHHVGLQEVDVRDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAF 461
             ++ RQG+ ++ L +VDV++ KT+ SVP DGKTMGEI+ +G+++M GY KN KAT EAF
Sbjct: 361 IELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAF 420

Query: 462 KGGWFHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVARP 521
           K GW ++GD  V HPD Y+E+KDR KD+IISGGENIS++EVE +L+ +  VLEAAVVA P
Sbjct: 421 KHGWLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMP 480

Query: 522 DDHWGETPCAFVVLKEGCNTVTAQQ--LIDYCRDRLPHYMAPRTIV-FQDLPKTSTGKVQ 581
              WGETPCAFVVLK+G   +   +  LI YCR+ +PH+M P+ +V FQ+LPK S GK+ 
Sbjct: 481 HPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKIL 540

Query: 582 KFILRERAKAM 585
           K  LR+ AKA+
Sbjct: 541 KSKLRDIAKAL 548

The following BLAST results are available for this feature:

BLAST of CSPI06G32380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SEY5	1.5e-229	64.01	Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1	[more]
M4IRL4	1.3e-225	68.11	Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1	[more]
M4IS92	1.4e-224	67.57	Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1	[more]
F4HUK6	3.0e-214	64.23	Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1	[more]
M4IS88	7.7e-170	51.25	Acetate--CoA ligase CCL3 OS=Humulus lupulus OX=3486 GN=CCL3 PE=1 SV=1	[more]

BLAST of CSPI06G32380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KGV7	0.0e+00	99.66	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G509050 PE=4 SV=1	[more]
A0A1S3B1R0	0.0e+00	97.62	probable acyl-activating enzyme 1, peroxisomal isoform X1 OS=Cucumis melo OX=365...	[more]
A0A5A7SME5	0.0e+00	97.45	Putative acyl-activating enzyme 1, peroxisomal isoform X1 OS=Cucumis melo var. m...	[more]
A0A6J1BTA3	0.0e+00	88.36	probable acyl-activating enzyme 1, peroxisomal isoform X1 OS=Momordica charantia...	[more]
A0A6J1KUK0	5.2e-302	84.89	probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of CSPI06G32380 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134830.2	0.0e+00	99.66	probable acyl-activating enzyme 2 isoform X1 [Cucumis sativus]	[more]
XP_008440901.1	0.0e+00	97.62	PREDICTED: probable acyl-activating enzyme 1, peroxisomal isoform X1 [Cucumis me...	[more]
KAA0025615.1	0.0e+00	97.45	putative acyl-activating enzyme 1, peroxisomal isoform X1 [Cucumis melo var. mak...	[more]
XP_038882615.1	0.0e+00	93.71	probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida]	[more]
XP_022132791.1	0.0e+00	88.36	probable acyl-activating enzyme 1, peroxisomal isoform X1 [Momordica charantia]	[more]

BLAST of CSPI06G32380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G17650.1	1.0e-230	64.01	AMP-dependent synthetase and ligase family protein	[more]
AT1G20560.1	2.1e-215	64.23	acyl activating enzyme 1	[more]
AT1G20560.2	4.6e-186	64.83	acyl activating enzyme 1	[more]
AT3G16910.1	1.2e-170	50.45	acyl-activating enzyme 7	[more]
AT1G66120.1	6.5e-164	50.09	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 61..487 e-value: 2.4E-90 score: 303.2
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 45..481 e-value: 2.7E-96 score: 324.7
None	No IPR available	GENE3D	3.30.300.30		coord: 482..585 e-value: 3.4E-31 score: 109.9
None	No IPR available	PANTHER	PTHR43859	ACYL-ACTIVATING ENZYME	coord: 27..587
None	No IPR available	PANTHER	PTHR43859:SF5	ACYL-ACTIVATING ENZYME 2-RELATED	coord: 27..587
None	No IPR available	CDD	cd12118	ttLC_FACS_AEE21_like	coord: 53..579 e-value: 0.0 score: 858.909
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 29..582
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 496..571 e-value: 2.4E-22 score: 79.7
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 230..241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G32380.1	CSPI06G32380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm