CSPI06G32270 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G32270
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDUF295 domain-containing protein
LocationChr6: 27228623 .. 27230511 (-)
RNA-Seq ExpressionCSPI06G32270
SyntenyCSPI06G32270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAACTTCAAAGTTAACTTGAGTCTCCAACCCTCATGTTAGATTGGGTTTTAGTGTTAAAATATCAAACAAGCAAGGATCTTCGATTTTTAATATCGGTTTGAAAGTTTTAGAAATGGGTCGTTGACGTCTAGCAATGCCAATTTATGAAGATTAGATAAAGTAAAAATTAAAAGTTGCTCCAATAGCAACATCGAATGTATGATATATATGCGGATATATAAAGAAAATTATATTGTAAACATATTTGTTGTTTATATCATCAAAGTTGGTTTCCGTAAGGGTCATAGCATAGGTCAATCCAATCCACTGATATTCTTTCTCCTCATCGTCTTTCTGTTGGACCAATCGGATTCAAGTCAAACTTCAGCCACCATGGAAGACAGCCGTGTACGGTGGTCCGATCTTCCTCCTGAACTTTGGCCAATCATCGGCAAACGCCTCGATACCTACATCGACGTCCTCCGATTCCGCAGTGTCTGCCGATCTTGGCGTGCTTCTCTACCTCCTTTCAACGCCGTTTCTCCTCTTCTACCTCTCGATCTCCCTTCTCCAGTCTTCGCTGCCGATCATCTCACAGACGCCTACCTTATCCGAAGAATAATCTACCGCCTCAGTCCTCTCGATCATCATCAAACTTTCGATTTTGCTTCTTCTTCTTCTTCTTCTTCATCATCATCATCTTCTTCTTTTTCTTGTCCTGCAGAGGGCTGGTTAGCGAAGGTCGAGAGTACAAAATTGGGTAAAATGCGCTTTCTCCATCCGCTTTCAACACGCTATGCTAAATGCAACTCCGATCTATTACGGAAGGAAGTGAACTTGCTTGATTTTGGAATTTACGAAGTAGCTAAATCTTATACTCTTGGTTATACTAATGGTTCTCTTGTTCCTCGAATTACTAAGGTTGTAATGTTCCCTGATTCCCCCTGGATTGATGTTAAGAAATGCACCATTTTAGCAATTTACGCCGGTGGAAAATTAGGGTTTGCTAAACATGGAGACAACAAGTGGACGCTGATCGACCATCGTAATTTCCACTACGACGATGTGATTGTGTACAAAGGGCAATTCTATGCAGTTGATCGATGGGGAACAATTTTCTGGATTGATTCATCCATGAAATTGGTACAGTTTTCTCCTCCATTATGTGGTTTTGGTAATCAAAAGCATTTGGTTGAATGTAACGGTGAACTCTATGTTGTTGATCGATTTCTGGATAAGGAACCATTGTTATGGAATGCTGATATCTTCCATATCCATTGGTTGAATAATCTCATTGAAGATTCCTCTCCTAAAGTAATTGATTTCAAACTTCATAGGCTTGATCAAGAGTGGGGAAGATGGGTTGAGGTAAAAAATTTGGGGAATGAATCCTTTGTTTTGGGAAATGATTGTTGTTTTTCAATTTCAACTCCAAAGTTTGAAGGACTTAAAGGGAGTTGCATATACTTTACTCATACACCAAAATGTGCTCTTGGTTATAACACTCTTGTATTTGAGCTTGAAGAGAAAAGGATTCTGAACGCCTTTTCAAATGATAATGCACCAATTTTTCGGCCTCCCCCAATTTGGCTCAATTTGGAGGCAACTCAGTTTGAAGAAGATGCAGCTGATAATCAATAAGGTATTGTATGAACTGAGATTTCATTTACAAATTAACGTTCGTATCTAAACAGTTTAGCATTCTCTTTGTTGTACACTCTGTTCATAGCTATTCAGGGTTGGAGTTTGTATTGTGGGTGTCAAGTTCTAATTAGTGATTTATGAATCTTGGTGTGGCTGTTGTGTAGTATGTAATAGTTTTAATTATGTGTAAATATGTATTATTAGGCATCATTATAAACTATGTTGTTTTTTATGACTTTGTTTGAATACTGGCTTCGGACCTA

mRNA sequence

AAGAACTTCAAAGTTAACTTGAGTCTCCAACCCTCATGTTAGATTGGGTTTTAGTGTTAAAATATCAAACAAGCAAGGATCTTCGATTTTTAATATCGGTTTGAAAGTTTTAGAAATGGGTCGTTGACGTCTAGCAATGCCAATTTATGAAGATTAGATAAAGTAAAAATTAAAAGTTGCTCCAATAGCAACATCGAATGTATGATATATATGCGGATATATAAAGAAAATTATATTGTAAACATATTTGTTGTTTATATCATCAAAGTTGGTTTCCGTAAGGGTCATAGCATAGGTCAATCCAATCCACTGATATTCTTTCTCCTCATCGTCTTTCTGTTGGACCAATCGGATTCAAGTCAAACTTCAGCCACCATGGAAGACAGCCGTGTACGGTGGTCCGATCTTCCTCCTGAACTTTGGCCAATCATCGGCAAACGCCTCGATACCTACATCGACGTCCTCCGATTCCGCAGTGTCTGCCGATCTTGGCGTGCTTCTCTACCTCCTTTCAACGCCGTTTCTCCTCTTCTACCTCTCGATCTCCCTTCTCCAGTCTTCGCTGCCGATCATCTCACAGACGCCTACCTTATCCGAAGAATAATCTACCGCCTCAGTCCTCTCGATCATCATCAAACTTTCGATTTTGCTTCTTCTTCTTCTTCTTCTTCATCATCATCATCTTCTTCTTTTTCTTGTCCTGCAGAGGGCTGGTTAGCGAAGGTCGAGAGTACAAAATTGGGTAAAATGCGCTTTCTCCATCCGCTTTCAACACGCTATGCTAAATGCAACTCCGATCTATTACGGAAGGAAGTGAACTTGCTTGATTTTGGAATTTACGAAGTAGCTAAATCTTATACTCTTGGTTATACTAATGGTTCTCTTGTTCCTCGAATTACTAAGGTTGTAATGTTCCCTGATTCCCCCTGGATTGATGTTAAGAAATGCACCATTTTAGCAATTTACGCCGGTGGAAAATTAGGGTTTGCTAAACATGGAGACAACAAGTGGACGCTGATCGACCATCGTAATTTCCACTACGACGATGTGATTGTGTACAAAGGGCAATTCTATGCAGTTGATCGATGGGGAACAATTTTCTGGATTGATTCATCCATGAAATTGGTACAGTTTTCTCCTCCATTATGTGGTTTTGGTAATCAAAAGCATTTGGTTGAATGTAACGGTGAACTCTATGTTGTTGATCGATTTCTGGATAAGGAACCATTGTTATGGAATGCTGATATCTTCCATATCCATTGGTTGAATAATCTCATTGAAGATTCCTCTCCTAAAGTAATTGATTTCAAACTTCATAGGCTTGATCAAGAGTGGGGAAGATGGGTTGAGGTAAAAAATTTGGGGAATGAATCCTTTGTTTTGGGAAATGATTGTTGTTTTTCAATTTCAACTCCAAAGTTTGAAGGACTTAAAGGGAGTTGCATATACTTTACTCATACACCAAAATGTGCTCTTGGTTATAACACTCTTGTATTTGAGCTTGAAGAGAAAAGGATTCTGAACGCCTTTTCAAATGATAATGCACCAATTTTTCGGCCTCCCCCAATTTGGCTCAATTTGGAGGCAACTCAGTTTGAAGAAGATGCAGCTGATAATCAATAAGGTATTGTATGAACTGAGATTTCATTTACAAATTAACGTTCGTATCTAAACAGTTTAGCATTCTCTTTGTTGTACACTCTGTTCATAGCTATTCAGGGTTGGAGTTTGTATTGTGGGTGTCAAGTTCTAATTAGTGATTTATGAATCTTGGTGTGGCTGTTGTGTAGTATGTAATAGTTTTAATTATGTGTAAATATGTATTATTAGGCATCATTATAAACTATGTTGTTTTTTATGACTTTGTTTGAATACTGGCTTCGGACCTA

Coding sequence (CDS)

ATGATATATATGCGGATATATAAAGAAAATTATATTGTAAACATATTTGTTGTTTATATCATCAAAGTTGGTTTCCGTAAGGGTCATAGCATAGGTCAATCCAATCCACTGATATTCTTTCTCCTCATCGTCTTTCTGTTGGACCAATCGGATTCAAGTCAAACTTCAGCCACCATGGAAGACAGCCGTGTACGGTGGTCCGATCTTCCTCCTGAACTTTGGCCAATCATCGGCAAACGCCTCGATACCTACATCGACGTCCTCCGATTCCGCAGTGTCTGCCGATCTTGGCGTGCTTCTCTACCTCCTTTCAACGCCGTTTCTCCTCTTCTACCTCTCGATCTCCCTTCTCCAGTCTTCGCTGCCGATCATCTCACAGACGCCTACCTTATCCGAAGAATAATCTACCGCCTCAGTCCTCTCGATCATCATCAAACTTTCGATTTTGCTTCTTCTTCTTCTTCTTCTTCATCATCATCATCTTCTTCTTTTTCTTGTCCTGCAGAGGGCTGGTTAGCGAAGGTCGAGAGTACAAAATTGGGTAAAATGCGCTTTCTCCATCCGCTTTCAACACGCTATGCTAAATGCAACTCCGATCTATTACGGAAGGAAGTGAACTTGCTTGATTTTGGAATTTACGAAGTAGCTAAATCTTATACTCTTGGTTATACTAATGGTTCTCTTGTTCCTCGAATTACTAAGGTTGTAATGTTCCCTGATTCCCCCTGGATTGATGTTAAGAAATGCACCATTTTAGCAATTTACGCCGGTGGAAAATTAGGGTTTGCTAAACATGGAGACAACAAGTGGACGCTGATCGACCATCGTAATTTCCACTACGACGATGTGATTGTGTACAAAGGGCAATTCTATGCAGTTGATCGATGGGGAACAATTTTCTGGATTGATTCATCCATGAAATTGGTACAGTTTTCTCCTCCATTATGTGGTTTTGGTAATCAAAAGCATTTGGTTGAATGTAACGGTGAACTCTATGTTGTTGATCGATTTCTGGATAAGGAACCATTGTTATGGAATGCTGATATCTTCCATATCCATTGGTTGAATAATCTCATTGAAGATTCCTCTCCTAAAGTAATTGATTTCAAACTTCATAGGCTTGATCAAGAGTGGGGAAGATGGGTTGAGGTAAAAAATTTGGGGAATGAATCCTTTGTTTTGGGAAATGATTGTTGTTTTTCAATTTCAACTCCAAAGTTTGAAGGACTTAAAGGGAGTTGCATATACTTTACTCATACACCAAAATGTGCTCTTGGTTATAACACTCTTGTATTTGAGCTTGAAGAGAAAAGGATTCTGAACGCCTTTTCAAATGATAATGCACCAATTTTTCGGCCTCCCCCAATTTGGCTCAATTTGGAGGCAACTCAGTTTGAAGAAGATGCAGCTGATAATCAATAA

Protein sequence

MIYMRIYKENYIVNIFVVYIIKVGFRKGHSIGQSNPLIFFLLIVFLLDQSDSSQTSATMEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSPVFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVESTKLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMFPDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGTIFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNLIEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIYFTHTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADNQ*
Homology
BLAST of CSPI06G32270 vs. ExPASy Swiss-Prot
Match: Q9SHX9 (Putative F-box protein At1g65770 OS=Arabidopsis thaliana OX=3702 GN=At1g65770 PE=4 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.8e-39
Identity = 114/359 (31.75%), Postives = 179/359 (49.86%), Query Frame = 0

Query: 66  WSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPL--LPLDLPSPVFAAD 125
           WS LP +L  +I  RL + I++ RFRS+CRSWR+S+P     +P    PL L +P     
Sbjct: 4   WSTLPVDLLNMIAGRLFSNIELKRFRSICRSWRSSVPGAGKKNPFRTRPLILLNP----- 63

Query: 126 HLTDAYLIRRIIYRLSPL-DHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVE-STKLG 185
                           PL DH +  +F S S+    + SSS   P++GWL K +     G
Sbjct: 64  ------------NPNKPLTDHRRRGEFLSRSAFFRVTLSSS---PSQGWLIKSDVDVSSG 123

Query: 186 KMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTL--GYTNGSLVPRITKVVMFP 245
           K+  L PLS    +   +  RK V+L +F I E+ ++Y +    T     P   +V +  
Sbjct: 124 KLHLLDPLS----RLPMEHSRKRVDLSEFTITEIREAYQVHDWRTRKETRPIFKRVALVK 183

Query: 246 DSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGTI 305
           D    +     +L I + GK+    + D K        + + D+IV+KGQ YA+D  G +
Sbjct: 184 DKEGDN----QVLGIRSTGKM---MYWDIKTWKAKEEGYEFSDIIVHKGQTYALDSIGIV 243

Query: 306 FWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNLI 365
           +WI S +K ++F P +  +   + LVEC GE Y+V+R + +      AD     +     
Sbjct: 244 YWIRSDLKFIRFGPLVGDWTGDRRLVECCGEFYIVERLVGESTWKRKADDTGYEY----- 303

Query: 366 EDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIYFT 419
                K + FK+++ D E G+ +EVK+LG+++FV+  D CFS+   +F G   + IYFT
Sbjct: 304 ----AKTVGFKVYKFDDEQGKMMEVKSLGDKAFVIATDTCFSVLAHEFYGCLENAIYFT 322

BLAST of CSPI06G32270 vs. ExPASy Swiss-Prot
Match: Q3EBY8 (F-box protein At2g17690 OS=Arabidopsis thaliana OX=3702 GN=SDC PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.6e-32
Identity = 116/405 (28.64%), Postives = 181/405 (44.69%), Query Frame = 0

Query: 66  WSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFN---AVSPLLPLDLPSPVFAA 125
           WS LP EL  +I  RL + I+++RFRS+C+SWR+S    N   ++S  L    P  +  A
Sbjct: 4   WSKLPEELLGLIALRLYSVIELIRFRSICKSWRSSASGVNKNHSLSSPLIYFKPLQIILA 63

Query: 126 DHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVESTKLGK 185
               +  ++ +  Y  + L     F    +SS            P +GWL K ++    K
Sbjct: 64  RAQANGQILSK--YHGTVLSRATFFRVTLASS------------PDQGWLIKSDTDL--K 123

Query: 186 MRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSY-TLGYTNGSLVPRITKVVMFPDS 245
            R  H L+    K      RK ++L +F + E+ +SY  +G           +V +    
Sbjct: 124 YRNFHLLNCLSRKA-LGRSRKLISLSEFIVSEIQESYAVVGRRTRETASEFKRVFLVR-- 183

Query: 246 PWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGTIFW 305
             +      +L I   GK+ F K G   W  I  +   + D ++ +G  YAVD  G ++W
Sbjct: 184 --VQGGDHRVLVIGIDGKIRFWKGG--IWNGIKKQVAQFSDFVLDEGLTYAVDTKGIMWW 243

Query: 306 IDSSMKLVQFSPPL-----CGFGNQKHLVECNGELYVVDRFLDKEPL------------- 365
           I S+  ++++   L      G   +K  V C GELY+VDR +D+  L             
Sbjct: 244 ISSAYDIIRYGTKLHENITNGSCGEKRFVRCRGELYIVDRLIDENLLKRKADSYDDNAIV 303

Query: 366 -----------LW-----NADIF------HIHWLNNLI--------EDSSPKVIDFKLHR 419
                      LW     N D F       IH L++ +        E   PK I FK+++
Sbjct: 304 HFRNADYTNADLWEDGNGNDDFFAAQAHRFIHVLHDNLGNVSCKPFERDPPKTIGFKVYK 363

BLAST of CSPI06G32270 vs. ExPASy Swiss-Prot
Match: Q3EBZ2 (F-box protein SKIP23 OS=Arabidopsis thaliana OX=3702 GN=SKIP23 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 8.7e-31
Identity = 120/423 (28.37%), Postives = 192/423 (45.39%), Query Frame = 0

Query: 64  VRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVS----PLLPLDLPSPV 123
           V WS LP +L  +I K L++  D+++FRSVC SWR++  P + +     P+LP D    +
Sbjct: 2   VDWSTLPKDLLDLISKSLESSFDLIQFRSVCSSWRSAAEPKSPLPTHHLPILP-DNGGSL 61

Query: 124 FAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVES-- 183
           F  D      L +R I  + P  H    +            S SF     GWL KVE   
Sbjct: 62  F-PDSAVGFRLSQRSILLIKP--HEPCIE------------SDSF-----GWLIKVEEDL 121

Query: 184 TKLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTN--GSLVPRI--- 243
               K+  L PL         +  R  +++  F + E+ + + L Y N  G +V  +   
Sbjct: 122 NVPRKVTLLDPLCDTRNSIPENFPR-VLDMSKFKVRELGREFKLHYFNTVGDIVESLYLE 181

Query: 244 TKVVMFPDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYA 303
             VV + D       K  +L I+  GKL   +  D  WT+I+     YDDV+++ G+F+A
Sbjct: 182 KAVVKYLDCD--GDYKFVLLTIHVSGKLAVFRSWDRAWTVINDMPSPYDDVMLFDGRFFA 241

Query: 304 VDRWGTIFWID-SSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFH 363
           VD  G    +D SS+KL   + P+ G G++K L+E  GE+ +VD +L  E +  +     
Sbjct: 242 VDNNGRTVVVDYSSLKLTLVASPVFG-GDKKFLIESCGEMLLVDMYLSLEAVEGDPG--- 301

Query: 364 IHWLNNLIED----SSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKF 423
             ++  + E      + + + FK++R  +    WV+V +L ++   LG+D  FS S    
Sbjct: 302 --FVEEIFEHPAFYMNERTVKFKVYRFVEREESWVDVYDLEDKMLFLGDDSTFSASASDI 361

Query: 424 EGL-KGSCIYFTHTPKCALGYNTL------VFELEEKRI-LNAFSNDNAPIFRPPPIWLN 463
             L  GS ++F            +      VF+    +I L     + A +F PPP W+ 
Sbjct: 362 LPLCDGSSVFFNGNVFNGEDLGAMQDRDLGVFDFRSGKIELVQKLPEYAKLFWPPPPWIT 394

BLAST of CSPI06G32270 vs. ExPASy Swiss-Prot
Match: Q9LVG8 (Putative F-box protein At5g60060 OS=Arabidopsis thaliana OX=3702 GN=At5g60060 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 5.7e-30
Identity = 112/376 (29.79%), Postives = 175/376 (46.54%), Query Frame = 0

Query: 65  RWSDLPPELWPIIGKRLD----TYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSPVF 124
           +WSDLP ++  +I  RLD      I +L  RSVC +WR SLP  N  +            
Sbjct: 10  QWSDLPLDILELISDRLDHDSSDTIHLLCLRSVCATWRLSLPLSNKNN------------ 69

Query: 125 AADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEG------WLAK 184
                           RLS    +  F  +SSSSS   +   S     E        L K
Sbjct: 70  ----------------RLSKFPKYLPFWSSSSSSSGFFTLKQSNVYKLEAPLNPRTCLVK 129

Query: 185 VESTKLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTN--GSLVP-- 244
           ++ T  G MR L   S        +    +++L +F +  V ++Y + Y N  G  VP  
Sbjct: 130 LQETTPGIMRVLDLFSNDRICFLPENFPSKIDLQEFHVRLVRRTYRMEYANNGGGAVPCF 189

Query: 245 ---RITKVVMFPDSPWIDVKKCTILAIYAGGKLGFAKHG-DNKWTLIDHR-NFHYDDVIV 304
                 KVV+         +   I+AI++GGKLGF K G D KW ++D+  N  Y+D+++
Sbjct: 190 WSLHSDKVVILSSG-----EDSAIIAIHSGGKLGFLKSGNDEKWKILDNSWNVIYEDIML 249

Query: 305 YK-GQFYAVDRWGTIFWIDSSMKLVQFSPPLC-GFGNQKHLVECN-GELYVVDRFLDKEP 364
           YK  +   VD  G     D   K+   +  L  G G++KHLVEC+ GE+++VD+++    
Sbjct: 250 YKDNRCIVVDDKGKTVIYDVDFKVSDLAEGLVGGGGHKKHLVECSGGEVFLVDKYVKTV- 309

Query: 365 LLWNADIFHIHWLNNLIEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSI 419
                      W  +   D S  V++F+++ L +E  RW EV++LG+ +  +G+DC FS+
Sbjct: 310 -----------WCKS---DISKSVVEFRVYNLKREEKRWEEVRDLGDVALFIGDDCSFSV 336

BLAST of CSPI06G32270 vs. ExPASy Swiss-Prot
Match: Q6DR20 (F-box protein At2g17036 OS=Arabidopsis thaliana OX=3702 GN=At2g17036 PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 4.8e-29
Identity = 116/412 (28.16%), Postives = 193/412 (46.84%), Query Frame = 0

Query: 66  WSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSPVFAADHL 125
           W+ LP +L  +I K L++  D+++FRSVC SWR++  P      L   +L  P F +D  
Sbjct: 4   WATLPKDLLDLISKCLESSFDLIQFRSVCSSWRSAAGPKRL---LWAHNL--PFFPSD-- 63

Query: 126 TDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAE--GWLAKVEST--KLG 185
            D   +  +I R++    HQ+      +            C A+  GW+ KV        
Sbjct: 64  -DKPFLSNVILRVA----HQSILLIKPNEP---------QCEADLFGWIVKVWDNIYVSR 123

Query: 186 KMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMFPDS 245
           KM  L PLS+        L R   ++  F + E+ +   L + +   VP  T + +    
Sbjct: 124 KMTLLKPLSSSRNYFPQHLPR-IFDMSKFTVRELCREVKLYHPDYYCVPGHTALELELGK 183

Query: 246 ---PWIDVKKCTILAIYAGGKLGFAKHGDNKWTLI-DHRNFHYDDVIVYKGQFYAVDRWG 305
               +++  K  +L I   GKL   +  D +WT+I D+      D+I++ G+F+A+D  G
Sbjct: 184 TVVKYLNDDKFVLLTILEYGKLAVFRSWDREWTVINDYIPSRCQDLIMFDGRFFAIDYNG 243

Query: 306 TIFWID-SSMKLVQFSPPLCGFGNQKHLVECNGELYVVD--RFLDKEPLLWNADIFHIHW 365
               +D SS KL   + PL G G++K L+E  GE+++VD    L+++P       F+ ++
Sbjct: 244 RTVVVDYSSFKLTLAANPLIGGGDKKFLIESCGEMFLVDIEFCLNEKPEFTGG--FYSYF 303

Query: 366 LNNLIEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSIST----PKFEGL 425
               +         FK  +L +   RWVEV++LG++ F LG+D  FS ST    P+  G 
Sbjct: 304 NETTVS------YKFKFFKLVEREKRWVEVEDLGDKMFFLGDDSTFSASTADIIPRCVGT 363

Query: 426 KGSCIYFTHTPKCAL--GYNTLVFELE--EKRILNAFSNDNAPIFRPPPIWL 459
                ++TH     +    N  VF+    +  ++N    + A +F PPP W+
Sbjct: 364 GSFVFFYTHEESLVVMDDRNLGVFDFRSGKTELVNKLP-EYAKLFWPPPPWI 384

BLAST of CSPI06G32270 vs. ExPASy TrEMBL
Match: A0A0A0KJJ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G507420 PE=4 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 6.3e-250
Identity = 411/415 (99.04%), Postives = 413/415 (99.52%), Query Frame = 0

Query: 59  MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 118
           MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP
Sbjct: 1   MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 60

Query: 119 VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST 178
           VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST
Sbjct: 61  VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST 120

Query: 179 KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF 238
           KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF
Sbjct: 121 KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF 180

Query: 239 PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGT 298
           PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDH NFHYDDVIVYKGQFYAVDRWGT
Sbjct: 181 PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHCNFHYDDVIVYKGQFYAVDRWGT 240

Query: 299 IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 358
           IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL
Sbjct: 241 IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 300

Query: 359 IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIYFT 418
           IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFS+STP F+GLKGSCIYFT
Sbjct: 301 IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSVSTPYFKGLKGSCIYFT 360

Query: 419 HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADNQ 474
           HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADNQ
Sbjct: 361 HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADNQ 415

BLAST of CSPI06G32270 vs. ExPASy TrEMBL
Match: A0A5A7SHJ7 (Putative F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00810 PE=4 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.2e-237
Identity = 389/415 (93.73%), Postives = 400/415 (96.39%), Query Frame = 0

Query: 59  MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 118
           MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP
Sbjct: 1   MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 60

Query: 119 VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST 178
           VFAADHLTDA+LIRRIIYRLSPLDHH+TF FASSS+SSSSSSS    C AEGWLAKVEST
Sbjct: 61  VFAADHLTDAFLIRRIIYRLSPLDHHKTFSFASSSTSSSSSSS---YCAAEGWLAKVEST 120

Query: 179 KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF 238
           KLGKMRFLHPLSTRYAKCNSDL RKEVNLLDFGIYEVAKSYTLGYTNGS VPRITKVVMF
Sbjct: 121 KLGKMRFLHPLSTRYAKCNSDLFRKEVNLLDFGIYEVAKSYTLGYTNGSPVPRITKVVMF 180

Query: 239 PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGT 298
           PDSPWIDVKKCTILA+YAGGKLGFAKHGD+KWTLIDHRNFHYDDVIVYKGQFYAVDRWGT
Sbjct: 181 PDSPWIDVKKCTILAVYAGGKLGFAKHGDDKWTLIDHRNFHYDDVIVYKGQFYAVDRWGT 240

Query: 299 IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 358
           +FWIDSSM+LVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL
Sbjct: 241 VFWIDSSMRLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 300

Query: 359 IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIYFT 418
           IEDS PKVIDFKLHRLDQEWGRWVEVKNLGN+SF+LGNDCCFS+STP FEGLKGSCIYFT
Sbjct: 301 IEDSPPKVIDFKLHRLDQEWGRWVEVKNLGNQSFILGNDCCFSVSTPNFEGLKGSCIYFT 360

Query: 419 HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADNQ 474
           HTPKCALGYNT VFELE+KRIL A SNDNAPIFRPPPIWLNLE TQ EEDAADNQ
Sbjct: 361 HTPKCALGYNTHVFELEDKRILKASSNDNAPIFRPPPIWLNLEVTQIEEDAADNQ 412

BLAST of CSPI06G32270 vs. ExPASy TrEMBL
Match: A0A1S4DT77 (putative F-box protein At1g65770 OS=Cucumis melo OX=3656 GN=LOC103485361 PE=4 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.2e-237
Identity = 389/415 (93.73%), Postives = 400/415 (96.39%), Query Frame = 0

Query: 59  MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 118
           MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP
Sbjct: 1   MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 60

Query: 119 VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST 178
           VFAADHLTDA+LIRRIIYRLSPLDHH+TF FASSS+SSSSSSS    C AEGWLAKVEST
Sbjct: 61  VFAADHLTDAFLIRRIIYRLSPLDHHKTFSFASSSTSSSSSSS---YCAAEGWLAKVEST 120

Query: 179 KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF 238
           KLGKMRFLHPLSTRYAKCNSDL RKEVNLLDFGIYEVAKSYTLGYTNGS VPRITKVVMF
Sbjct: 121 KLGKMRFLHPLSTRYAKCNSDLFRKEVNLLDFGIYEVAKSYTLGYTNGSPVPRITKVVMF 180

Query: 239 PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGT 298
           PDSPWIDVKKCTILA+YAGGKLGFAKHGD+KWTLIDHRNFHYDDVIVYKGQFYAVDRWGT
Sbjct: 181 PDSPWIDVKKCTILAVYAGGKLGFAKHGDDKWTLIDHRNFHYDDVIVYKGQFYAVDRWGT 240

Query: 299 IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 358
           +FWIDSSM+LVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL
Sbjct: 241 VFWIDSSMRLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 300

Query: 359 IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIYFT 418
           IEDS PKVIDFKLHRLDQEWGRWVEVKNLGN+SF+LGNDCCFS+STP FEGLKGSCIYFT
Sbjct: 301 IEDSPPKVIDFKLHRLDQEWGRWVEVKNLGNQSFILGNDCCFSVSTPNFEGLKGSCIYFT 360

Query: 419 HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADNQ 474
           HTPKCALGYNT VFELE+KRIL A SNDNAPIFRPPPIWLNLE TQ EEDAADNQ
Sbjct: 361 HTPKCALGYNTHVFELEDKRILKASSNDNAPIFRPPPIWLNLEVTQIEEDAADNQ 412

BLAST of CSPI06G32270 vs. ExPASy TrEMBL
Match: A0A6J1EGF3 (putative F-box protein At1g65770 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433939 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 2.3e-204
Identity = 340/414 (82.13%), Postives = 368/414 (88.89%), Query Frame = 0

Query: 59  MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 118
           M+DSRV+WSDLPPE+ PIIGK L+TYI+VLRFRSVCRSWRASLPPFN VS LLPLDLPSP
Sbjct: 1   MDDSRVQWSDLPPEILPIIGKSLETYIEVLRFRSVCRSWRASLPPFNIVSSLLPLDLPSP 60

Query: 119 VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST 178
           +F ADHLTDA LIRR+IYRLSPLDH           SS S+SSSSF+  AEGWLAKVEST
Sbjct: 61  IFTADHLTDALLIRRVIYRLSPLDHQH---------SSDSASSSSFA--AEGWLAKVEST 120

Query: 179 KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF 238
           KLG MRFLHPLSTRY K ++++ RKEVNLLDF IYEVAKSYTLGYTNG+LVPRITKVVMF
Sbjct: 121 KLGTMRFLHPLSTRYVKYDNEVFRKEVNLLDFRIYEVAKSYTLGYTNGALVPRITKVVMF 180

Query: 239 PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGT 298
           PD PWIDVK CTI+A+YAGGKLGFAKHGD KWTLID RNFHYDDVIVYKGQFYAVDRWGT
Sbjct: 181 PDCPWIDVKYCTIVAVYAGGKLGFAKHGDYKWTLIDDRNFHYDDVIVYKGQFYAVDRWGT 240

Query: 299 IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 358
           IFWIDSSM+LVQFSPPLCGFGNQKHLVE + ELYVVDRFLDKEPLLWNADIFHIHWLNNL
Sbjct: 241 IFWIDSSMRLVQFSPPLCGFGNQKHLVESDDELYVVDRFLDKEPLLWNADIFHIHWLNNL 300

Query: 359 IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIYFT 418
           IEDS PKVIDFK+HRLDQEWGRWVEV+NLGN+SFVLGNDCCFSIS PKFEG+KGSCIYFT
Sbjct: 301 IEDSPPKVIDFKVHRLDQEWGRWVEVRNLGNQSFVLGNDCCFSISAPKFEGVKGSCIYFT 360

Query: 419 HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADN 473
           H P+  LGYNT  FEL+ KRI  A  +DN PIF PPPIWLNLEAT++EE A+DN
Sbjct: 361 HAPRSTLGYNTHYFELKYKRIAKASIHDNEPIFGPPPIWLNLEATRYEEHASDN 403

BLAST of CSPI06G32270 vs. ExPASy TrEMBL
Match: A0A6J1KNW7 (putative F-box protein At1g65770 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497348 PE=4 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 5.2e-204
Identity = 339/414 (81.88%), Postives = 366/414 (88.41%), Query Frame = 0

Query: 59  MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 118
           M+DSRV+WSDLPPE+ PIIGK L+TYIDVLRFRSVCRSWRASLPPFN VS LLPLDLPSP
Sbjct: 1   MDDSRVQWSDLPPEILPIIGKSLETYIDVLRFRSVCRSWRASLPPFNIVSSLLPLDLPSP 60

Query: 119 VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST 178
           +F ADHLTDA LIRR+IYRLSPLDH           SS S+SSSSF+  AEGWLAKVEST
Sbjct: 61  IFTADHLTDALLIRRVIYRLSPLDHQH---------SSDSASSSSFA--AEGWLAKVEST 120

Query: 179 KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF 238
           KLG MRFLHPLSTRY K ++++ RKEVNLLDF IYEVAKSYTLGY NG+LVPRITKVVMF
Sbjct: 121 KLGTMRFLHPLSTRYVKYDNEVFRKEVNLLDFRIYEVAKSYTLGYINGALVPRITKVVMF 180

Query: 239 PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGT 298
           PD PWIDVK CTI+A+YAGGKLGFAKHGD KWTLID RNFHYDDVIVYKGQFYAVDRWGT
Sbjct: 181 PDCPWIDVKYCTIVAVYAGGKLGFAKHGDYKWTLIDDRNFHYDDVIVYKGQFYAVDRWGT 240

Query: 299 IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 358
           IFWIDSSM+LVQFSPPLCGFGNQKH VE + ELYVVDRFLDKEPLLWNADIFHIHWLNNL
Sbjct: 241 IFWIDSSMRLVQFSPPLCGFGNQKHFVESDDELYVVDRFLDKEPLLWNADIFHIHWLNNL 300

Query: 359 IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIYFT 418
           IEDS PKVIDFK+HRLDQEWGRWVEV+NLGN+SFVLGNDCCFS+S PKFEG+KGSCIYFT
Sbjct: 301 IEDSPPKVIDFKVHRLDQEWGRWVEVRNLGNQSFVLGNDCCFSVSAPKFEGVKGSCIYFT 360

Query: 419 HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADN 473
           H P+  LGYNT  FEL+ KRI  A  +DN PIF PPPIWLNLEATQ+EE A+DN
Sbjct: 361 HAPRSTLGYNTHYFELKYKRIAKASIHDNEPIFGPPPIWLNLEATQYEEHASDN 403

BLAST of CSPI06G32270 vs. NCBI nr
Match: XP_004134837.2 (putative F-box protein At1g65770 [Cucumis sativus] >KGN48969.1 hypothetical protein Csa_003175 [Cucumis sativus])

HSP 1 Score: 872.8 bits (2254), Expect = 1.3e-249
Identity = 411/415 (99.04%), Postives = 413/415 (99.52%), Query Frame = 0

Query: 59  MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 118
           MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP
Sbjct: 1   MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 60

Query: 119 VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST 178
           VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST
Sbjct: 61  VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST 120

Query: 179 KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF 238
           KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF
Sbjct: 121 KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF 180

Query: 239 PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGT 298
           PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDH NFHYDDVIVYKGQFYAVDRWGT
Sbjct: 181 PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHCNFHYDDVIVYKGQFYAVDRWGT 240

Query: 299 IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 358
           IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL
Sbjct: 241 IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 300

Query: 359 IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIYFT 418
           IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFS+STP F+GLKGSCIYFT
Sbjct: 301 IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSVSTPYFKGLKGSCIYFT 360

Query: 419 HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADNQ 474
           HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADNQ
Sbjct: 361 HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADNQ 415

BLAST of CSPI06G32270 vs. NCBI nr
Match: XP_016899202.1 (PREDICTED: putative F-box protein At1g65770 [Cucumis melo] >KAA0025628.1 putative F-box protein [Cucumis melo var. makuwa] >TYK12503.1 putative F-box protein [Cucumis melo var. makuwa])

HSP 1 Score: 832.0 bits (2148), Expect = 2.5e-237
Identity = 389/415 (93.73%), Postives = 400/415 (96.39%), Query Frame = 0

Query: 59  MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 118
           MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP
Sbjct: 1   MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 60

Query: 119 VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST 178
           VFAADHLTDA+LIRRIIYRLSPLDHH+TF FASSS+SSSSSSS    C AEGWLAKVEST
Sbjct: 61  VFAADHLTDAFLIRRIIYRLSPLDHHKTFSFASSSTSSSSSSS---YCAAEGWLAKVEST 120

Query: 179 KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF 238
           KLGKMRFLHPLSTRYAKCNSDL RKEVNLLDFGIYEVAKSYTLGYTNGS VPRITKVVMF
Sbjct: 121 KLGKMRFLHPLSTRYAKCNSDLFRKEVNLLDFGIYEVAKSYTLGYTNGSPVPRITKVVMF 180

Query: 239 PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGT 298
           PDSPWIDVKKCTILA+YAGGKLGFAKHGD+KWTLIDHRNFHYDDVIVYKGQFYAVDRWGT
Sbjct: 181 PDSPWIDVKKCTILAVYAGGKLGFAKHGDDKWTLIDHRNFHYDDVIVYKGQFYAVDRWGT 240

Query: 299 IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 358
           +FWIDSSM+LVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL
Sbjct: 241 VFWIDSSMRLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 300

Query: 359 IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIYFT 418
           IEDS PKVIDFKLHRLDQEWGRWVEVKNLGN+SF+LGNDCCFS+STP FEGLKGSCIYFT
Sbjct: 301 IEDSPPKVIDFKLHRLDQEWGRWVEVKNLGNQSFILGNDCCFSVSTPNFEGLKGSCIYFT 360

Query: 419 HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADNQ 474
           HTPKCALGYNT VFELE+KRIL A SNDNAPIFRPPPIWLNLE TQ EEDAADNQ
Sbjct: 361 HTPKCALGYNTHVFELEDKRILKASSNDNAPIFRPPPIWLNLEVTQIEEDAADNQ 412

BLAST of CSPI06G32270 vs. NCBI nr
Match: XP_038881861.1 (putative F-box protein At1g65770 [Benincasa hispida])

HSP 1 Score: 790.8 bits (2041), Expect = 6.5e-225
Identity = 375/418 (89.71%), Postives = 392/418 (93.78%), Query Frame = 0

Query: 59  MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 118
           MEDSRVRWSDLPPELWPIIGKRLDT+ID+LRFRSVCRSWRASLPPFNA+SPLLPLDLPSP
Sbjct: 1   MEDSRVRWSDLPPELWPIIGKRLDTHIDILRFRSVCRSWRASLPPFNAISPLLPLDLPSP 60

Query: 119 VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFA--SSSSSSSSSSSSSFSCPAEGWLAKVE 178
           VFAADHLTDA+LIRRIIYRLSPL H QT D A  SSSSSSSSSSSSS SC AEGWLAKVE
Sbjct: 61  VFAADHLTDAFLIRRIIYRLSPLHHRQTSDSASSSSSSSSSSSSSSSSSCAAEGWLAKVE 120

Query: 179 STKLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVV 238
           STKLGKMRFLHPLSTRYAK N++L RKEVNLLDFGIYEVAKSYTLGYTNG+LVPRITKVV
Sbjct: 121 STKLGKMRFLHPLSTRYAKYNNELFRKEVNLLDFGIYEVAKSYTLGYTNGALVPRITKVV 180

Query: 239 MFPDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRW 298
           MFPDSPWIDVK CTILA+YAGGKLGFAKHGD+KWTLID RNFHYDDVIVYKGQFYAVDRW
Sbjct: 181 MFPDSPWIDVKNCTILAVYAGGKLGFAKHGDDKWTLIDDRNFHYDDVIVYKGQFYAVDRW 240

Query: 299 GTIFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLN 358
           GT+FWIDSSMKLVQFSPPLCGFGNQKHLVE  GELYVVDRFLDKEP LWNADIFHIHWLN
Sbjct: 241 GTVFWIDSSMKLVQFSPPLCGFGNQKHLVESGGELYVVDRFLDKEPSLWNADIFHIHWLN 300

Query: 359 NLIEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIY 418
           NLIEDS PKVIDFKLHRLDQEWGRWVEVK+LGN+SFVLGNDCCFS+S+P FEG+KGSCIY
Sbjct: 301 NLIEDSPPKVIDFKLHRLDQEWGRWVEVKSLGNQSFVLGNDCCFSVSSPHFEGVKGSCIY 360

Query: 419 FTHTPKCALGYNTLVFELEEKRILNAFSN--DNAPIFRPPPIWLNLEATQFEEDAADN 473
           FTHTPKC LGYNT VFELEEKRIL A  N  DNAPIF PPPIWLNLEAT F+EDAAD+
Sbjct: 361 FTHTPKCTLGYNTYVFELEEKRILKASINDIDNAPIFGPPPIWLNLEATHFQEDAADD 418

BLAST of CSPI06G32270 vs. NCBI nr
Match: XP_023518907.1 (putative F-box protein At1g65770 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 723.4 bits (1866), Expect = 1.3e-204
Identity = 341/414 (82.37%), Postives = 367/414 (88.65%), Query Frame = 0

Query: 59  MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 118
           M+DSRV+WSDLPPE+ PIIGK L+TYIDVLRFRSVCRSWRASLPPFN VS LLPLDLPSP
Sbjct: 1   MDDSRVQWSDLPPEILPIIGKSLETYIDVLRFRSVCRSWRASLPPFNVVSSLLPLDLPSP 60

Query: 119 VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST 178
           +F ADHLTDA LIRR+IYRLSPLDH           SS S+SSSSF+  AEGWLAKVEST
Sbjct: 61  IFTADHLTDALLIRRVIYRLSPLDHQH---------SSDSASSSSFA--AEGWLAKVEST 120

Query: 179 KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF 238
           KLG MRFLHPLSTRY K ++++ RKEVNLLDF IYEVAKSYTLGYTNG+LVPRITKVVMF
Sbjct: 121 KLGTMRFLHPLSTRYVKYDNEVFRKEVNLLDFRIYEVAKSYTLGYTNGALVPRITKVVMF 180

Query: 239 PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGT 298
           PD PWIDVK CTI+A+YAGGKLGFAKHGD KWTLID RNFHYDDVIVYKGQFYAVDRWGT
Sbjct: 181 PDCPWIDVKYCTIVAVYAGGKLGFAKHGDYKWTLIDDRNFHYDDVIVYKGQFYAVDRWGT 240

Query: 299 IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 358
           IFWIDSSM+LVQFSPPLCGFGNQKHLVE + ELYVVDRFLDKEPLLWNADIFHIHWLNNL
Sbjct: 241 IFWIDSSMRLVQFSPPLCGFGNQKHLVESDDELYVVDRFLDKEPLLWNADIFHIHWLNNL 300

Query: 359 IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIYFT 418
           IEDS PKVIDFK+HRLDQEWGRWVEV+NLGN+SFVLGNDCCFSIS PKFEG+KGSCIYFT
Sbjct: 301 IEDSPPKVIDFKVHRLDQEWGRWVEVRNLGNQSFVLGNDCCFSISAPKFEGVKGSCIYFT 360

Query: 419 HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADN 473
           H P+  LGYNT  FEL+ KRI  A  +DN PIF PPPIWLN EATQ+EE A+DN
Sbjct: 361 HAPRSTLGYNTHYFELKYKRIAKASIHDNEPIFGPPPIWLNFEATQYEEHASDN 403

BLAST of CSPI06G32270 vs. NCBI nr
Match: KAG6594746.1 (putative F-box protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 721.8 bits (1862), Expect = 3.7e-204
Identity = 341/414 (82.37%), Postives = 367/414 (88.65%), Query Frame = 0

Query: 59  MEDSRVRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSP 118
           M+DSRV+WSDLPPE+ PIIGK L+TYIDVLRFRSVCRSWRASLPPFN VS LLPLDLPSP
Sbjct: 1   MDDSRVQWSDLPPEILPIIGKSLETYIDVLRFRSVCRSWRASLPPFNIVSSLLPLDLPSP 60

Query: 119 VFAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVEST 178
           +F ADHLTDA LIRR+IYRLSPLDH           SS S+SSSSF+  AEGWLAKVEST
Sbjct: 61  IFTADHLTDALLIRRVIYRLSPLDHQH---------SSDSASSSSFA--AEGWLAKVEST 120

Query: 179 KLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMF 238
           KLG MRFLHPLSTRY K ++++ RKEVNLLDF IYEVAKSYTLGYTNG+LVPRITKVVMF
Sbjct: 121 KLGTMRFLHPLSTRYVKYDNEVFRKEVNLLDFRIYEVAKSYTLGYTNGALVPRITKVVMF 180

Query: 239 PDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGT 298
           PD PWIDVK CTI+A+YAGGKLGFAKHGD KWTLID RNFHYDDVIVYKGQFYAVDRWGT
Sbjct: 181 PDCPWIDVKYCTIVAVYAGGKLGFAKHGDYKWTLIDDRNFHYDDVIVYKGQFYAVDRWGT 240

Query: 299 IFWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNL 358
           IFWIDSSM+LVQFSPPLCGFGNQKHLVE + ELYVVDRFLDKEPLLWNADIFHIHWLNNL
Sbjct: 241 IFWIDSSMRLVQFSPPLCGFGNQKHLVESDDELYVVDRFLDKEPLLWNADIFHIHWLNNL 300

Query: 359 IEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIYFT 418
           IEDS PKVIDFK+HRLDQEWGRWVEV+NLGN+SFVLGNDCCFSIS PKFEG+KGSCIYFT
Sbjct: 301 IEDSPPKVIDFKVHRLDQEWGRWVEVRNLGNQSFVLGNDCCFSISAPKFEGVKGSCIYFT 360

Query: 419 HTPKCALGYNTLVFELEEKRILNAFSNDNAPIFRPPPIWLNLEATQFEEDAADN 473
           H P+  LGYNT  FEL+ KRI  A  +DN PIF PPPIWLNLEATQ+E  A+DN
Sbjct: 361 HAPRSTLGYNTHYFELKYKRIAKASIHDNEPIFGPPPIWLNLEATQYEGHASDN 403

BLAST of CSPI06G32270 vs. TAIR 10
Match: AT1G65770.1 (ascorbic acid mannose pathway regulator 1 )

HSP 1 Score: 165.2 bits (417), Expect = 1.2e-40
Identity = 114/359 (31.75%), Postives = 179/359 (49.86%), Query Frame = 0

Query: 66  WSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPL--LPLDLPSPVFAAD 125
           WS LP +L  +I  RL + I++ RFRS+CRSWR+S+P     +P    PL L +P     
Sbjct: 4   WSTLPVDLLNMIAGRLFSNIELKRFRSICRSWRSSVPGAGKKNPFRTRPLILLNP----- 63

Query: 126 HLTDAYLIRRIIYRLSPL-DHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVE-STKLG 185
                           PL DH +  +F S S+    + SSS   P++GWL K +     G
Sbjct: 64  ------------NPNKPLTDHRRRGEFLSRSAFFRVTLSSS---PSQGWLIKSDVDVSSG 123

Query: 186 KMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTL--GYTNGSLVPRITKVVMFP 245
           K+  L PLS    +   +  RK V+L +F I E+ ++Y +    T     P   +V +  
Sbjct: 124 KLHLLDPLS----RLPMEHSRKRVDLSEFTITEIREAYQVHDWRTRKETRPIFKRVALVK 183

Query: 246 DSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGTI 305
           D    +     +L I + GK+    + D K        + + D+IV+KGQ YA+D  G +
Sbjct: 184 DKEGDN----QVLGIRSTGKM---MYWDIKTWKAKEEGYEFSDIIVHKGQTYALDSIGIV 243

Query: 306 FWIDSSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFHIHWLNNLI 365
           +WI S +K ++F P +  +   + LVEC GE Y+V+R + +      AD     +     
Sbjct: 244 YWIRSDLKFIRFGPLVGDWTGDRRLVECCGEFYIVERLVGESTWKRKADDTGYEY----- 303

Query: 366 EDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKFEGLKGSCIYFT 419
                K + FK+++ D E G+ +EVK+LG+++FV+  D CFS+   +F G   + IYFT
Sbjct: 304 ----AKTVGFKVYKFDDEQGKMMEVKSLGDKAFVIATDTCFSVLAHEFYGCLENAIYFT 322

BLAST of CSPI06G32270 vs. TAIR 10
Match: AT2G17690.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-33
Identity = 116/405 (28.64%), Postives = 181/405 (44.69%), Query Frame = 0

Query: 66  WSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFN---AVSPLLPLDLPSPVFAA 125
           WS LP EL  +I  RL + I+++RFRS+C+SWR+S    N   ++S  L    P  +  A
Sbjct: 4   WSKLPEELLGLIALRLYSVIELIRFRSICKSWRSSASGVNKNHSLSSPLIYFKPLQIILA 63

Query: 126 DHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVESTKLGK 185
               +  ++ +  Y  + L     F    +SS            P +GWL K ++    K
Sbjct: 64  RAQANGQILSK--YHGTVLSRATFFRVTLASS------------PDQGWLIKSDTDL--K 123

Query: 186 MRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSY-TLGYTNGSLVPRITKVVMFPDS 245
            R  H L+    K      RK ++L +F + E+ +SY  +G           +V +    
Sbjct: 124 YRNFHLLNCLSRKA-LGRSRKLISLSEFIVSEIQESYAVVGRRTRETASEFKRVFLVR-- 183

Query: 246 PWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYAVDRWGTIFW 305
             +      +L I   GK+ F K G   W  I  +   + D ++ +G  YAVD  G ++W
Sbjct: 184 --VQGGDHRVLVIGIDGKIRFWKGG--IWNGIKKQVAQFSDFVLDEGLTYAVDTKGIMWW 243

Query: 306 IDSSMKLVQFSPPL-----CGFGNQKHLVECNGELYVVDRFLDKEPL------------- 365
           I S+  ++++   L      G   +K  V C GELY+VDR +D+  L             
Sbjct: 244 ISSAYDIIRYGTKLHENITNGSCGEKRFVRCRGELYIVDRLIDENLLKRKADSYDDNAIV 303

Query: 366 -----------LW-----NADIF------HIHWLNNLI--------EDSSPKVIDFKLHR 419
                      LW     N D F       IH L++ +        E   PK I FK+++
Sbjct: 304 HFRNADYTNADLWEDGNGNDDFFAAQAHRFIHVLHDNLGNVSCKPFERDPPKTIGFKVYK 363

BLAST of CSPI06G32270 vs. TAIR 10
Match: AT2G17030.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 136.3 bits (342), Expect = 6.2e-32
Identity = 120/423 (28.37%), Postives = 192/423 (45.39%), Query Frame = 0

Query: 64  VRWSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVS----PLLPLDLPSPV 123
           V WS LP +L  +I K L++  D+++FRSVC SWR++  P + +     P+LP D    +
Sbjct: 2   VDWSTLPKDLLDLISKSLESSFDLIQFRSVCSSWRSAAEPKSPLPTHHLPILP-DNGGSL 61

Query: 124 FAADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEGWLAKVES-- 183
           F  D      L +R I  + P  H    +            S SF     GWL KVE   
Sbjct: 62  F-PDSAVGFRLSQRSILLIKP--HEPCIE------------SDSF-----GWLIKVEEDL 121

Query: 184 TKLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTN--GSLVPRI--- 243
               K+  L PL         +  R  +++  F + E+ + + L Y N  G +V  +   
Sbjct: 122 NVPRKVTLLDPLCDTRNSIPENFPR-VLDMSKFKVRELGREFKLHYFNTVGDIVESLYLE 181

Query: 244 TKVVMFPDSPWIDVKKCTILAIYAGGKLGFAKHGDNKWTLIDHRNFHYDDVIVYKGQFYA 303
             VV + D       K  +L I+  GKL   +  D  WT+I+     YDDV+++ G+F+A
Sbjct: 182 KAVVKYLDCD--GDYKFVLLTIHVSGKLAVFRSWDRAWTVINDMPSPYDDVMLFDGRFFA 241

Query: 304 VDRWGTIFWID-SSMKLVQFSPPLCGFGNQKHLVECNGELYVVDRFLDKEPLLWNADIFH 363
           VD  G    +D SS+KL   + P+ G G++K L+E  GE+ +VD +L  E +  +     
Sbjct: 242 VDNNGRTVVVDYSSLKLTLVASPVFG-GDKKFLIESCGEMLLVDMYLSLEAVEGDPG--- 301

Query: 364 IHWLNNLIED----SSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSISTPKF 423
             ++  + E      + + + FK++R  +    WV+V +L ++   LG+D  FS S    
Sbjct: 302 --FVEEIFEHPAFYMNERTVKFKVYRFVEREESWVDVYDLEDKMLFLGDDSTFSASASDI 361

Query: 424 EGL-KGSCIYFTHTPKCALGYNTL------VFELEEKRI-LNAFSNDNAPIFRPPPIWLN 463
             L  GS ++F            +      VF+    +I L     + A +F PPP W+ 
Sbjct: 362 LPLCDGSSVFFNGNVFNGEDLGAMQDRDLGVFDFRSGKIELVQKLPEYAKLFWPPPPWIT 394

BLAST of CSPI06G32270 vs. TAIR 10
Match: AT5G60060.1 (Protein of unknown function (DUF295) )

HSP 1 Score: 133.7 bits (335), Expect = 4.0e-31
Identity = 112/376 (29.79%), Postives = 175/376 (46.54%), Query Frame = 0

Query: 65  RWSDLPPELWPIIGKRLD----TYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSPVF 124
           +WSDLP ++  +I  RLD      I +L  RSVC +WR SLP  N  +            
Sbjct: 10  QWSDLPLDILELISDRLDHDSSDTIHLLCLRSVCATWRLSLPLSNKNN------------ 69

Query: 125 AADHLTDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAEG------WLAK 184
                           RLS    +  F  +SSSSS   +   S     E        L K
Sbjct: 70  ----------------RLSKFPKYLPFWSSSSSSSGFFTLKQSNVYKLEAPLNPRTCLVK 129

Query: 185 VESTKLGKMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTN--GSLVP-- 244
           ++ T  G MR L   S        +    +++L +F +  V ++Y + Y N  G  VP  
Sbjct: 130 LQETTPGIMRVLDLFSNDRICFLPENFPSKIDLQEFHVRLVRRTYRMEYANNGGGAVPCF 189

Query: 245 ---RITKVVMFPDSPWIDVKKCTILAIYAGGKLGFAKHG-DNKWTLIDHR-NFHYDDVIV 304
                 KVV+         +   I+AI++GGKLGF K G D KW ++D+  N  Y+D+++
Sbjct: 190 WSLHSDKVVILSSG-----EDSAIIAIHSGGKLGFLKSGNDEKWKILDNSWNVIYEDIML 249

Query: 305 YK-GQFYAVDRWGTIFWIDSSMKLVQFSPPLC-GFGNQKHLVECN-GELYVVDRFLDKEP 364
           YK  +   VD  G     D   K+   +  L  G G++KHLVEC+ GE+++VD+++    
Sbjct: 250 YKDNRCIVVDDKGKTVIYDVDFKVSDLAEGLVGGGGHKKHLVECSGGEVFLVDKYVKTV- 309

Query: 365 LLWNADIFHIHWLNNLIEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSI 419
                      W  +   D S  V++F+++ L +E  RW EV++LG+ +  +G+DC FS+
Sbjct: 310 -----------WCKS---DISKSVVEFRVYNLKREEKRWEEVRDLGDVALFIGDDCSFSV 336

BLAST of CSPI06G32270 vs. TAIR 10
Match: AT2G17036.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 130.6 bits (327), Expect = 3.4e-30
Identity = 116/412 (28.16%), Postives = 193/412 (46.84%), Query Frame = 0

Query: 66  WSDLPPELWPIIGKRLDTYIDVLRFRSVCRSWRASLPPFNAVSPLLPLDLPSPVFAADHL 125
           W+ LP +L  +I K L++  D+++FRSVC SWR++  P      L   +L  P F +D  
Sbjct: 3   WATLPKDLLDLISKCLESSFDLIQFRSVCSSWRSAAGPKRL---LWAHNL--PFFPSD-- 62

Query: 126 TDAYLIRRIIYRLSPLDHHQTFDFASSSSSSSSSSSSSFSCPAE--GWLAKVEST--KLG 185
            D   +  +I R++    HQ+      +            C A+  GW+ KV        
Sbjct: 63  -DKPFLSNVILRVA----HQSILLIKPNEP---------QCEADLFGWIVKVWDNIYVSR 122

Query: 186 KMRFLHPLSTRYAKCNSDLLRKEVNLLDFGIYEVAKSYTLGYTNGSLVPRITKVVMFPDS 245
           KM  L PLS+        L R   ++  F + E+ +   L + +   VP  T + +    
Sbjct: 123 KMTLLKPLSSSRNYFPQHLPR-IFDMSKFTVRELCREVKLYHPDYYCVPGHTALELELGK 182

Query: 246 ---PWIDVKKCTILAIYAGGKLGFAKHGDNKWTLI-DHRNFHYDDVIVYKGQFYAVDRWG 305
               +++  K  +L I   GKL   +  D +WT+I D+      D+I++ G+F+A+D  G
Sbjct: 183 TVVKYLNDDKFVLLTILEYGKLAVFRSWDREWTVINDYIPSRCQDLIMFDGRFFAIDYNG 242

Query: 306 TIFWID-SSMKLVQFSPPLCGFGNQKHLVECNGELYVVD--RFLDKEPLLWNADIFHIHW 365
               +D SS KL   + PL G G++K L+E  GE+++VD    L+++P       F+ ++
Sbjct: 243 RTVVVDYSSFKLTLAANPLIGGGDKKFLIESCGEMFLVDIEFCLNEKPEFTGG--FYSYF 302

Query: 366 LNNLIEDSSPKVIDFKLHRLDQEWGRWVEVKNLGNESFVLGNDCCFSIST----PKFEGL 425
               +         FK  +L +   RWVEV++LG++ F LG+D  FS ST    P+  G 
Sbjct: 303 NETTVS------YKFKFFKLVEREKRWVEVEDLGDKMFFLGDDSTFSASTADIIPRCVGT 362

Query: 426 KGSCIYFTHTPKCAL--GYNTLVFELE--EKRILNAFSNDNAPIFRPPPIWL 459
                ++TH     +    N  VF+    +  ++N    + A +F PPP W+
Sbjct: 363 GSFVFFYTHEESLVVMDDRNLGVFDFRSGKTELVNKLP-EYAKLFWPPPPWI 383

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SHX91.8e-3931.75Putative F-box protein At1g65770 OS=Arabidopsis thaliana OX=3702 GN=At1g65770 PE... [more]
Q3EBY81.6e-3228.64F-box protein At2g17690 OS=Arabidopsis thaliana OX=3702 GN=SDC PE=2 SV=1[more]
Q3EBZ28.7e-3128.37F-box protein SKIP23 OS=Arabidopsis thaliana OX=3702 GN=SKIP23 PE=1 SV=1[more]
Q9LVG85.7e-3029.79Putative F-box protein At5g60060 OS=Arabidopsis thaliana OX=3702 GN=At5g60060 PE... [more]
Q6DR204.8e-2928.16F-box protein At2g17036 OS=Arabidopsis thaliana OX=3702 GN=At2g17036 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KJJ16.3e-25099.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G507420 PE=4 SV=1[more]
A0A5A7SHJ71.2e-23793.73Putative F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
A0A1S4DT771.2e-23793.73putative F-box protein At1g65770 OS=Cucumis melo OX=3656 GN=LOC103485361 PE=4 SV... [more]
A0A6J1EGF32.3e-20482.13putative F-box protein At1g65770 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1KNW75.2e-20481.88putative F-box protein At1g65770 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_004134837.21.3e-24999.04putative F-box protein At1g65770 [Cucumis sativus] >KGN48969.1 hypothetical prot... [more]
XP_016899202.12.5e-23793.73PREDICTED: putative F-box protein At1g65770 [Cucumis melo] >KAA0025628.1 putativ... [more]
XP_038881861.16.5e-22589.71putative F-box protein At1g65770 [Benincasa hispida][more]
XP_023518907.11.3e-20482.37putative F-box protein At1g65770 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6594746.13.7e-20482.37putative F-box protein, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G65770.11.2e-4031.75ascorbic acid mannose pathway regulator 1 [more]
AT2G17690.11.1e-3328.64F-box family protein with a domain of unknown function (DUF295) [more]
AT2G17030.16.2e-3228.37F-box family protein with a domain of unknown function (DUF295) [more]
AT5G60060.14.0e-3129.79Protein of unknown function (DUF295) [more]
AT2G17036.13.4e-3028.16F-box family protein with a domain of unknown function (DUF295) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainPFAMPF00646F-boxcoord: 66..99
e-value: 3.6E-4
score: 20.3
NoneNo IPR availableGENE3D1.20.1280.50coord: 59..102
e-value: 1.2E-5
score: 27.0
NoneNo IPR availablePANTHERPTHR47123F-BOX PROTEIN SKIP23coord: 58..463
NoneNo IPR availablePANTHERPTHR47123:SF6ASCORBIC ACID MANNOSE PATHWAY REGULATOR-RELATEDcoord: 58..463
IPR005174Domain unknown function DUF295PFAMPF03478DUF295coord: 382..421
e-value: 1.2E-11
score: 44.0
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 58..99

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G32270.1CSPI06G32270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding