CSPI06G32160 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G32160
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr6: 27183800 .. 27187020 (-)
RNA-Seq Expression	CSPI06G32160
Synteny	CSPI06G32160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAGCCGCCGGGAAGCGAGAGAGAAAAATAAAAGAAAAAGAAGTTTGTGAGATTGGTGGAAAGGAGAGGCAGAACCCAAAATCAATCTTTAATTACGGTGTCAAAGTTCTTCGAGTTTACCATTACACAGAAGTTGAGAGAGAGAGAATTTGAGACTTTGAGAATTAGAGAATTAGAGGCTTTGGACTTTGGAGACGAAGACCAAAATTTTCAAACCACTACTCTCATCCAAATCCCCATCTTCAATTCCAGATCTTCCCCCCTAAAGGGTTTCTGTTTCTGTTTCCATGGCGAAACACAGACAATCTCGATTCCCTACTCGCAAGTCCTCTTCTTCCTCTACTCTCGTCTTTACCTTGCTCATTATGTTCACTTTCGTCATTCTCATTCTTCTCGCCCTTGGAATCCTCTCGATCCCTGGGAATTCCGGCGGTTCAACCAAGGTTCATGATCTCAGCTCGATTGTGCGCAAAACTTCTGATGAGTATGTGATCTTCTGGGTCTTGCTTTCAATTTCTTGTTCGGTTTAGGGTGTTTTTTTTTTTTTATTGATTATAAGGGTTTTGTTTGTTTGTTTGTTTTTTTACTTTTCATTTCAGCGTCGACGAGGAGAAAGGCGAGCAGTGGGTTGAAGTGATCTCATGGGAACCTAGAGCTTTCGTTTATCACAACTTTCTGGTATTCTCTTCTTCCCGATCTGTAATGTGAGAGATTTGTGTTTTATAAATATATATGCAACTTTTGCGTGGAATTTTTTAAACTTGGTTTGTTTATGCTCTTACGTGTTGAATGTGTCTTTGGGAGCGCATTTTTTAAATGAAGTCTTTAGGGGCTGCTCATCACTTAGGTTGATCATGTTTATGTTCCTCTAAGAAGTCATTGTGCTTCTTTGTTATTATGATGAAAGGGGTTAAGGATAATTCCCATTTTTATATGTCTTTAGATCGTGTTGGAGTTTTGAGTTGACTTAGGCTGAGGCTCGTCGATTAATCTTGGAGTAGGAAAATACTGAACTGGTTTACTTTTTAATTGCTTAGACGAAGGAGGAATGTGAATACCTAATCAGCCTTGCCAAGCCTCATATGCAAAAGTCTACAGTTGTTGACAGTGAAACTGGACAGAGTAAAGATAGCAGGTGTGCTATTCATTTCTTCATAATTCCTTTTTGTTAGTATTGCTGGATCTACAATGTAGAAGCCTCCCTGACCTATTTTTCAGAGTTCGCACTAGCTCTGGAACGTTTTTGCCGAGAGGACGTGATAAGACTGTTAGAACTATAGAGAAAAGGCTTTCTGATTTCTCCTTCATACCCGTAGGTACATTTTTTAGCCTCTTTCCTTCTTCCCGGTTTCTTCCCATTACATCAAGCATTATAATTTGATTATGCATGGTTAAAATTGAACTTTGTAATCTTTAATTTAAATCCAACTGCACTCATCATTGTGTTATAAATTTCCCCTCTTACCTAGCATTGCCAATCATGACTTTTATATTGTTAGAAAAAAGATTATTACAATCATGCTCGAGATCATTCAAATAGGCAGAATTATTGTGCTAAGTTTCACGTGCCAAGGAAGATCTTATAACTATGGCTGAAATGATCAATAATGAATATCAACTCCAATTGTTATCCTATGAGCTTATTACCCACCCACTGTCGCTTGTAAGAATGAAAGATAATTGGATTCACCTGATAAGCTGCTTCAATATTTGTTATTATATGTAGTTTTTGTTATCTTATCTTTGAAATTTTGCATTAGATCAAACTTATTAGACACCTTCTTTTTCATATTTTGTTACTCAAGTGCACACATAATATGCAGAGCACGGAGAAGGACTTCAGGTTCTTCATTACGAAGTAGGACAAAAATATGAACCTCATTTTGATTACTTCCTTGATGAGTACAATACAAAGAATGGAGGTCAACGTATAGCAACGGTGCTTATGTACCTGTAAGTAGTAATTGATATGAACTCAGAACAAGCGCTACTACATTTTGCATGGTTATCTCATCATTTTATATATCTTCTATCATAATTCAGCTCAGATGTCGAAGAAGGAGGTGAGACAGTGTTCCCTGCTGCCAAAGGAAACTTTAGTTCTGTACCTTGGTGGAATGAGCTTTCAGATTGTGGGAAGAAAGGACTTTCAGTTAAACCAAAGAGGGGCGATGCATTGCTTTTCTGGAGCATGAAGCCTGATGCTTCCCTAGATCCATCAAGTTTGCATGGTTAGATTGGATACTCTCAACTTATAAACTTACCATTCATTTTACTGAGCATGATATATAGGTTTCAGTGATGCACATTCATATCTGTCGATGCAATTTCTTTTGCATCTCCTATATGCAGAAAGTGATTGATTTTCTTGATAAAGAGATGACATTTTTTCTGGTGTTCATTTATAGGTGGCTGTCCTGTTATCAAGGGGAACAAATGGTCTGCTACTAAATGGATGCGAGTAGAAGAATATAAAGCTTGAGTTGATGTGACTAAGGTAATTTCCATGCTCTAAACCATGAACTAGAATTGTAATCAAATGAACACCTCAATCACTGGACAGTCATTCTTTAATTTTTCCTAGATCGTTATTCACCTGAATTGTTGAGGGTCTGTTTAATAATAGTTTTTGATCATTAAGCTTGTTTCCTCTTAATTTTTACAATGTTCATTTTTCTTTAGTAAAAAAGAGCTGAGTTCTTTTCCAAGTTCCAAAAATAAAAACAAGTTTTTAAAAGCTATTACTTTTTAGTTCTCAAAAGTTGATTGGTTCTTTAAAACATTAGTAAAATGTAGATAATAATTCAAAAAGTTTAGAGTTGGAAGCGATGTTTGTAGGCTTTTAACTTTTAAAAACTGAAAAAATAAATAACTAAATGGTTAACAAACGGGACTAAATCACTTTCAAGAAACGAACAAAGGAATTTTTTGTTCTGACTTTTCTCATGTTACTGGTAAAAGAATTATTCCTAAAGATTTGATGCTTCATTATCTTGCAGGTTGGTTGACATTTCTTGTTATTTGTCAAAGTCACAGATGCAATTTGTGTAGGGAATCATCATTTTCTATAATTTAACCTTTGTCCACAACTACTAGATCCTTAAATCTATGAATTTTGATATGGTTAAATTTTAGTTACGAGCTTACATTGTTATTGATTTCTTGTTTATGGCCACGAGAGAATAAGAGTTCATATTTTTGCCAGAAAATTCCACCT

mRNA sequence

AAAAAAAGCCGCCGGGAAGCGAGAGAGAAAAATAAAAGAAAAAGAAGTTTGTGAGATTGGTGGAAAGGAGAGGCAGAACCCAAAATCAATCTTTAATTACGGTGTCAAAGTTCTTCGAGTTTACCATTACACAGAAGTTGAGAGAGAGAGAATTTGAGACTTTGAGAATTAGAGAATTAGAGGCTTTGGACTTTGGAGACGAAGACCAAAATTTTCAAACCACTACTCTCATCCAAATCCCCATCTTCAATTCCAGATCTTCCCCCCTAAAGGGTTTCTGTTTCTGTTTCCATGGCGAAACACAGACAATCTCGATTCCCTACTCGCAAGTCCTCTTCTTCCTCTACTCTCGTCTTTACCTTGCTCATTATGTTCACTTTCGTCATTCTCATTCTTCTCGCCCTTGGAATCCTCTCGATCCCTGGGAATTCCGGCGGTTCAACCAAGGTTCATGATCTCAGCTCGATTGTGCGCAAAACTTCTGATGACGTCGACGAGGAGAAAGGCGAGCAGTGGGTTGAAGTGATCTCATGGGAACCTAGAGCTTTCGTTTATCACAACTTTCTGACGAAGGAGGAATGTGAATACCTAATCAGCCTTGCCAAGCCTCATATGCAAAAGTCTACAGTTGTTGACAGTGAAACTGGACAGAGTAAAGATAGCAGAGTTCGCACTAGCTCTGGAACGTTTTTGCCGAGAGGACGTGATAAGACTGTTAGAACTATAGAGAAAAGGCTTTCTGATTTCTCCTTCATACCCGTAGAGCACGGAGAAGGACTTCAGGTTCTTCATTACGAAGTAGGACAAAAATATGAACCTCATTTTGATTACTTCCTTGATGAGTACAATACAAAGAATGGAGGTCAACGTATAGCAACGGTGCTTATGTACCTCTCAGATGTCGAAGAAGGAGGTGAGACAGTGTTCCCTGCTGCCAAAGGAAACTTTAGTTCTGTACCTTGGTGGAATGAGCTTTCAGATTGTGGGAAGAAAGGACTTTCAGTTAAACCAAAGAGGGGCGATGCATTGCTTTTCTGGAGCATGAAGCCTGATGCTTCCCTAGATCCATCAAGTTTGCATGGTGGCTGTCCTGTTATCAAGGGGAACAAATGGTCTGCTACTAAATGGATGCGAGTAGAAGAATATAAAGCTTGAGTTGATGTGACTAAGGTTGGTTGACATTTCTTGTTATTTGTCAAAGTCACAGATGCAATTTGTGTAGGGAATCATCATTTTCTATAATTTAACCTTTGTCCACAACTACTAGATCCTTAAATCTATGAATTTTGATATGGTTAAATTTTAGTTACGAGCTTACATTGTTATTGATTTCTTGTTTATGGCCACGAGAGAATAAGAGTTCATATTTTTGCCAGAAAATTCCACCT

Coding sequence (CDS)

ATGGCGAAACACAGACAATCTCGATTCCCTACTCGCAAGTCCTCTTCTTCCTCTACTCTCGTCTTTACCTTGCTCATTATGTTCACTTTCGTCATTCTCATTCTTCTCGCCCTTGGAATCCTCTCGATCCCTGGGAATTCCGGCGGTTCAACCAAGGTTCATGATCTCAGCTCGATTGTGCGCAAAACTTCTGATGACGTCGACGAGGAGAAAGGCGAGCAGTGGGTTGAAGTGATCTCATGGGAACCTAGAGCTTTCGTTTATCACAACTTTCTGACGAAGGAGGAATGTGAATACCTAATCAGCCTTGCCAAGCCTCATATGCAAAAGTCTACAGTTGTTGACAGTGAAACTGGACAGAGTAAAGATAGCAGAGTTCGCACTAGCTCTGGAACGTTTTTGCCGAGAGGACGTGATAAGACTGTTAGAACTATAGAGAAAAGGCTTTCTGATTTCTCCTTCATACCCGTAGAGCACGGAGAAGGACTTCAGGTTCTTCATTACGAAGTAGGACAAAAATATGAACCTCATTTTGATTACTTCCTTGATGAGTACAATACAAAGAATGGAGGTCAACGTATAGCAACGGTGCTTATGTACCTCTCAGATGTCGAAGAAGGAGGTGAGACAGTGTTCCCTGCTGCCAAAGGAAACTTTAGTTCTGTACCTTGGTGGAATGAGCTTTCAGATTGTGGGAAGAAAGGACTTTCAGTTAAACCAAAGAGGGGCGATGCATTGCTTTTCTGGAGCATGAAGCCTGATGCTTCCCTAGATCCATCAAGTTTGCATGGTGGCTGTCCTGTTATCAAGGGGAACAAATGGTCTGCTACTAAATGGATGCGAGTAGAAGAATATAAAGCTTGA

Protein sequence

MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIVRKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQSKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA*

Homology

[Close]

BLAST of CSPI06G32160 vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 9.1e-132
Identity = 227/288 (78.82%), Postives = 256/288 (88.89%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MA+ R  R P+ + SS STLVF +LIM TFVILILLA GILS+P N+ GS+K +DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKT--SDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSET 120
           RKT      D+ K E+WVE+ISWEPRA VYHNFLTKEEC+YLI LAKPHM+KSTVVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GQSKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHF 180
           G+S DSRVRTSSGTFL RGRDKT+R IEKR+SDF+FIPVEHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSV 240
           DYF+DEYNT+NGGQRIATVLMYLSDVEEGGETVFPAAKGN+S+VPWWNELS+CGK GLSV
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSV 241

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           KPK GDALLFWSM PDA+LDPSSLHGGC VIKGNKWS+TKW+RV EYK
Sbjct: 242 KPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHEYK 288

[Close]

BLAST of CSPI06G32160 vs. ExPASy Swiss-Prot
Match: Q24JN5 (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 2.1e-120
Identity = 210/281 (74.73%), Postives = 241/281 (85.77%), Query Frame = 0


Query: 8   RFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIVRK--TSD 67
           R+  RKS S ST  FT+LI+   VILILL LGILS+P  +  S+K +DL++IVRK  TS 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTNIVRKSETSS 69

Query: 68  DVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQSKDSR 127
             +E  GE+WVEVISWEPRA VYHNFLT EECE+LISLAKP M KSTVVD +TG SKDSR
Sbjct: 70  GDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSR 129

Query: 128 VRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDYFLDEY 187
           VRTSSGTFL RG D+ V  IEKR+SDF+FIPVE+GEGLQVLHY+VGQKYEPH+DYFLDE+
Sbjct: 130 VRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFLDEF 189

Query: 188 NTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKPKRGDA 247
           NTKNGGQRIATVLMYLSDV++GGETVFPAA+GN S+VPWWNELS CGK+GLSV PK+ DA
Sbjct: 190 NTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDA 249

Query: 248 LLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           LLFW+M+PDASLDPSSLHGGCPV+KGNKWS+TKW  V E+K
Sbjct: 250 LLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEFK 290

[Close]

BLAST of CSPI06G32160 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 1.8e-119
Identity = 208/288 (72.22%), Postives = 248/288 (86.11%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MAK R SRF  RK S+   ++F +L M T V+L+LLA G+ S+P N+  S+ + DLS   
Sbjct: 1   MAKLRHSRFQARKWSTLMLVLF-MLFMLTIVLLMLLAFGVFSLPINNDESSPI-DLSYFR 60

Query: 61  RKTSDDVD--EEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSET 120
           R  ++  +   ++G+QW EV+SWEPRAFVYHNFL+KEECEYLISLAKPHM KSTVVDSET
Sbjct: 61  RAATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSET 120

Query: 121 GQSKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHF 180
           G+SKDSRVRTSSGTFL RGRDK ++TIEKR++D++FIP +HGEGLQVLHYE GQKYEPH+
Sbjct: 121 GKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHY 180

Query: 181 DYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSV 240
           DYF+DE+NTKNGGQR+AT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELS+CGKKGLSV
Sbjct: 181 DYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSV 240

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           KP+ GDALLFWSM+PDA+LDP+SLHGGCPVI+GNKWS+TKWM V EYK
Sbjct: 241 KPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEYK 286

[Close]

BLAST of CSPI06G32160 vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 2.3e-111
Identity = 199/288 (69.10%), Postives = 233/288 (80.90%), Query Frame = 0


Query: 3   KHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV-- 62
           K +Q R   RKS S+ T  FT++++  FVILIL+ LGI S+P  +  S+   DL++IV  
Sbjct: 4   KPKQLRNKPRKSFSTQT--FTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQT 63

Query: 63  ---RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSE 122
              R++  D ++  G++W+EVISWEPRAFVYHNFLT EECE+LISLAKP M KS VVD +
Sbjct: 64  IQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVK 123

Query: 123 TGQSKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPH 182
           TG+S DSRVRTSSGTFL RG D+ V  IE R+SDF+FIP E+GEGLQVLHYEVGQ+YEPH
Sbjct: 124 TGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPH 183

Query: 183 FDYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLS 242
            DYF DE+N + GGQRIATVLMYLSDV+EGGETVFPAAKGN S VPWW+ELS CGK+GLS
Sbjct: 184 HDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLS 243

Query: 243 VKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEY 286
           V PK+ DALLFWSMKPDASLDPSSLHGGCPVIKGNKWS+TKW  V EY
Sbjct: 244 VLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

[Close]

BLAST of CSPI06G32160 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 6.6e-66
Identity = 123/238 (51.68%), Postives = 174/238 (73.11%), Query Frame = 0


Query: 50  STKVHDLSSIVRKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQ 109
           S  +   +S++  +S  V+  K    V+ +S +PRAFVY  FLT+ EC++++SLAK  ++
Sbjct: 15  SVLLQSSTSLISSSSVFVNPSK----VKQVSSKPRAFVYEGFLTELECDHMVSLAKASLK 74

Query: 110 KSTVVDSETGQSKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYE 169
           +S V D+++G+SK S VRTSSGTF+ +G+D  V  IE ++S ++F+P E+GE +QVL YE
Sbjct: 75  RSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYE 134

Query: 170 VGQKYEPHFDYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWN--E 229
            GQKY+ HFDYF D+ N   GG R+AT+LMYLS+V +GGETVFP A+     V   N  +
Sbjct: 135 HGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKED 194

Query: 230 LSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEY 286
           LSDC K+G++VKP++GDALLF+++ PDA  DP SLHGGCPVI+G KWSATKW+ V+ +
Sbjct: 195 LSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHVDSF 248

[Close]

BLAST of CSPI06G32160 vs. ExPASy TrEMBL
Match: A0A0A0KJH9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507320 PE=4 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 2.7e-163
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120
           RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of CSPI06G32160 vs. ExPASy TrEMBL
Match: A0A1S3B1P6 (probable prolyl 4-hydroxylase 10 OS=Cucumis melo OX=3656 GN=LOC103485167 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 7.9e-163
Identity = 284/287 (98.95%), Postives = 287/287 (100.00%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120
           RKTSDDVDEEKGEQWVEVISWEPRAF+YHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFIYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKT+RTIEKR+SDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRISDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of CSPI06G32160 vs. ExPASy TrEMBL
Match: A0A6J1IRF1 (probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111478689 PE=4 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.6e-155
Identity = 269/287 (93.73%), Postives = 281/287 (97.91%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSST++FTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120
           RKTS+DVDEEKGE+W EVISWEPRAFVYHNFLTKEECEYLIS AKPHMQKSTVVDSETG+
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRG DK + TIEKR++DF+FIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLD+YNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 287

[Close]

BLAST of CSPI06G32160 vs. ExPASy TrEMBL
Match: A0A6J1GDV6 (probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111453285 PE=4 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 4.6e-155
Identity = 269/287 (93.73%), Postives = 280/287 (97.56%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MAKHRQSR PTRKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRLPTRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120
           RKTS+DVDEEKGE+W EVISWEPRAFVYHNFLTKEECEYLIS AKPHMQKSTVVDSETG+
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRG DK + TIEKR++DF+FIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLD+YNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 287

[Close]

BLAST of CSPI06G32160 vs. ExPASy TrEMBL
Match: A0A6J1EFG6 (probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111433654 PE=4 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.1e-153
Identity = 268/287 (93.38%), Postives = 280/287 (97.56%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MAKHRQ RFP+RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNS G+ K HDLSSIV
Sbjct: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNS-GAPKAHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120
           RKTSD+VDEEKGEQW EVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKS+VVDSETG+
Sbjct: 61  RKTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDK +R IEKR++DFSF+PVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLDEYNTKNGGQRIAT+LMYLSDVEEGGETVFPAAKGNFSSVPWW+ELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 286

[Close]

BLAST of CSPI06G32160 vs. NCBI nr
Match: XP_004134841.1 (probable prolyl 4-hydroxylase 10 [Cucumis sativus] >XP_031742781.1 probable prolyl 4-hydroxylase 10 [Cucumis sativus] >KGN48959.1 hypothetical protein Csa_003630 [Cucumis sativus])

HSP 1 Score: 584.3 bits (1505), Expect = 5.6e-163
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120
           RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of CSPI06G32160 vs. NCBI nr
Match: XP_008440878.1 (PREDICTED: probable prolyl 4-hydroxylase 10 [Cucumis melo])

HSP 1 Score: 582.8 bits (1501), Expect = 1.6e-162
Identity = 284/287 (98.95%), Postives = 287/287 (100.00%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120
           RKTSDDVDEEKGEQWVEVISWEPRAF+YHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFIYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKT+RTIEKR+SDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRISDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of CSPI06G32160 vs. NCBI nr
Match: XP_038881910.1 (probable prolyl 4-hydroxylase 10 isoform X2 [Benincasa hispida])

HSP 1 Score: 569.7 bits (1467), Expect = 1.4e-158
Identity = 277/286 (96.85%), Postives = 284/286 (99.30%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120
           RKTSDDVDEE+GEQWVEVISWEPRAFVYHNFLTKEECEYLISLA PHMQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEQGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAMPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKT+RTIEKR++DFSFIPVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLDEYNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGNFSSVPWWNELS+CGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWNELSECGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 286

[Close]

BLAST of CSPI06G32160 vs. NCBI nr
Match: XP_038881909.1 (probable prolyl 4-hydroxylase 10 isoform X1 [Benincasa hispida])

HSP 1 Score: 562.4 bits (1448), Expect = 2.3e-156
Identity = 276/290 (95.17%), Postives = 284/290 (97.93%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDD----VDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDS 120
           RKTSD+    VDEE+GEQWVEVISWEPRAFVYHNFLTKEECEYLISLA PHMQKSTVVDS
Sbjct: 61  RKTSDEYVISVDEEQGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAMPHMQKSTVVDS 120

Query: 121 ETGQSKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEP 180
           ETG+SKDSRVRTSSGTFLPRGRDKT+RTIEKR++DFSFIPVEHGEGLQVLHYEVGQKYEP
Sbjct: 121 ETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEP 180

Query: 181 HFDYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGL 240
           HFDYFLDEYNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGNFSSVPWWNELS+CGKKGL
Sbjct: 181 HFDYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWNELSECGKKGL 240

Query: 241 SVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           SVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK
Sbjct: 241 SVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 290

[Close]

BLAST of CSPI06G32160 vs. NCBI nr
Match: XP_022978860.1 (probable prolyl 4-hydroxylase 10 [Cucurbita maxima])

HSP 1 Score: 558.5 bits (1438), Expect = 3.3e-155
Identity = 269/287 (93.73%), Postives = 281/287 (97.91%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSST++FTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120
           RKTS+DVDEEKGE+W EVISWEPRAFVYHNFLTKEECEYLIS AKPHMQKSTVVDSETG+
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRG DK + TIEKR++DF+FIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLD+YNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 287

[Close]

BLAST of CSPI06G32160 vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 471.1 bits (1211), Expect = 6.4e-133
Identity = 227/288 (78.82%), Postives = 256/288 (88.89%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MA+ R  R P+ + SS STLVF +LIM TFVILILLA GILS+P N+ GS+K +DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKT--SDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSET 120
           RKT      D+ K E+WVE+ISWEPRA VYHNFLTKEEC+YLI LAKPHM+KSTVVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GQSKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHF 180
           G+S DSRVRTSSGTFL RGRDKT+R IEKR+SDF+FIPVEHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSV 240
           DYF+DEYNT+NGGQRIATVLMYLSDVEEGGETVFPAAKGN+S+VPWWNELS+CGK GLSV
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSV 241

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           KPK GDALLFWSM PDA+LDPSSLHGGC VIKGNKWS+TKW+RV EYK
Sbjct: 242 KPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHEYK 288

[Close]

BLAST of CSPI06G32160 vs. TAIR 10
Match: AT2G17720.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 433.3 bits (1113), Expect = 1.5e-121
Identity = 210/281 (74.73%), Postives = 241/281 (85.77%), Query Frame = 0


Query: 8   RFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIVRK--TSD 67
           R+  RKS S ST  FT+LI+   VILILL LGILS+P  +  S+K +DL++IVRK  TS 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTNIVRKSETSS 69

Query: 68  DVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQSKDSR 127
             +E  GE+WVEVISWEPRA VYHNFLT EECE+LISLAKP M KSTVVD +TG SKDSR
Sbjct: 70  GDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSR 129

Query: 128 VRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDYFLDEY 187
           VRTSSGTFL RG D+ V  IEKR+SDF+FIPVE+GEGLQVLHY+VGQKYEPH+DYFLDE+
Sbjct: 130 VRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFLDEF 189

Query: 188 NTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKPKRGDA 247
           NTKNGGQRIATVLMYLSDV++GGETVFPAA+GN S+VPWWNELS CGK+GLSV PK+ DA
Sbjct: 190 NTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDA 249

Query: 248 LLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           LLFW+M+PDASLDPSSLHGGCPV+KGNKWS+TKW  V E+K
Sbjct: 250 LLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEFK 290

[Close]

BLAST of CSPI06G32160 vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 430.3 bits (1105), Expect = 1.3e-120
Identity = 208/288 (72.22%), Postives = 248/288 (86.11%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MAK R SRF  RK S+   ++F +L M T V+L+LLA G+ S+P N+  S+ + DLS   
Sbjct: 1   MAKLRHSRFQARKWSTLMLVLF-MLFMLTIVLLMLLAFGVFSLPINNDESSPI-DLSYFR 60

Query: 61  RKTSDDVD--EEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSET 120
           R  ++  +   ++G+QW EV+SWEPRAFVYHNFL+KEECEYLISLAKPHM KSTVVDSET
Sbjct: 61  RAATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSET 120

Query: 121 GQSKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHF 180
           G+SKDSRVRTSSGTFL RGRDK ++TIEKR++D++FIP +HGEGLQVLHYE GQKYEPH+
Sbjct: 121 GKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHY 180

Query: 181 DYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSV 240
           DYF+DE+NTKNGGQR+AT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELS+CGKKGLSV
Sbjct: 181 DYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSV 240

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           KP+ GDALLFWSM+PDA+LDP+SLHGGCPVI+GNKWS+TKWM V EYK
Sbjct: 241 KPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEYK 286

[Close]

BLAST of CSPI06G32160 vs. TAIR 10
Match: AT4G35810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 403.3 bits (1035), Expect = 1.7e-112
Identity = 199/288 (69.10%), Postives = 233/288 (80.90%), Query Frame = 0


Query: 3   KHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV-- 62
           K +Q R   RKS S+ T  FT++++  FVILIL+ LGI S+P  +  S+   DL++IV  
Sbjct: 4   KPKQLRNKPRKSFSTQT--FTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQT 63

Query: 63  ---RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSE 122
              R++  D ++  G++W+EVISWEPRAFVYHNFLT EECE+LISLAKP M KS VVD +
Sbjct: 64  IQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVK 123

Query: 123 TGQSKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPH 182
           TG+S DSRVRTSSGTFL RG D+ V  IE R+SDF+FIP E+GEGLQVLHYEVGQ+YEPH
Sbjct: 124 TGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPH 183

Query: 183 FDYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLS 242
            DYF DE+N + GGQRIATVLMYLSDV+EGGETVFPAAKGN S VPWW+ELS CGK+GLS
Sbjct: 184 HDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLS 243

Query: 243 VKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEY 286
           V PK+ DALLFWSMKPDASLDPSSLHGGCPVIKGNKWS+TKW  V EY
Sbjct: 244 VLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

[Close]

BLAST of CSPI06G32160 vs. TAIR 10
Match: AT5G66060.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 363.6 bits (932), Expect = 1.5e-100
Identity = 181/237 (76.37%), Postives = 207/237 (87.34%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60
           MA+ R  R P+ + SS STLVF +LIM TFVILILLA GILS+P N+ GS+K +DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKT--SDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSET 120
           RKT      D+ K E+WVE+ISWEPRA VYHNFL  EEC+YLI LAKPHM+KSTVVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFL--EECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GQSKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHF 180
           G+S DSRVRTSSGTFL RGRDKT+R IEKR+SDF+FIPVEHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKG 236
           DYF+DEYNT+NGGQRIATVLMYLSDVEEGGETVFPAAKGN+S+VPWWNELS+CGK G
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGG 235

The following BLAST results are available for this feature:

BLAST of CSPI06G32160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JZ24	9.1e-132	78.82	Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S...	[more]
Q24JN5	2.1e-120	74.73	Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1	[more]
Q9LN20	1.8e-119	72.22	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]
F4JNU8	2.3e-111	69.10	Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=...	[more]
Q8LAN3	6.6e-66	51.68	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]

BLAST of CSPI06G32160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KJH9	2.7e-163	100.00	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A1S3B1P6	7.9e-163	98.95	probable prolyl 4-hydroxylase 10 OS=Cucumis melo OX=3656 GN=LOC103485167 PE=4 SV...	[more]
A0A6J1IRF1	1.6e-155	93.73	probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111478689 PE=...	[more]
A0A6J1GDV6	4.6e-155	93.73	probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111453285 P...	[more]
A0A6J1EFG6	1.1e-153	93.38	probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111433654 P...	[more]

BLAST of CSPI06G32160 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134841.1	5.6e-163	100.00	probable prolyl 4-hydroxylase 10 [Cucumis sativus] >XP_031742781.1 probable prol...	[more]
XP_008440878.1	1.6e-162	98.95	PREDICTED: probable prolyl 4-hydroxylase 10 [Cucumis melo]	[more]
XP_038881910.1	1.4e-158	96.85	probable prolyl 4-hydroxylase 10 isoform X2 [Benincasa hispida]	[more]
XP_038881909.1	2.3e-156	95.17	probable prolyl 4-hydroxylase 10 isoform X1 [Benincasa hispida]	[more]
XP_022978860.1	3.3e-155	93.73	probable prolyl 4-hydroxylase 10 [Cucurbita maxima]	[more]

BLAST of CSPI06G32160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66060.1	6.4e-133	78.82	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G17720.1	1.5e-121	74.73	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G20270.1	1.3e-120	72.22	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G35810.1	1.7e-112	69.10	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G66060.2	1.5e-100	76.37	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 83..281 e-value: 2.3E-65 score: 233.1
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 163..281 e-value: 5.8E-22 score: 78.4
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 75..282 e-value: 1.2E-81 score: 275.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 112..133
None	No IPR available	PANTHER	PTHR10869:SF157	PROLYL 4-HYDROXYLASE 10-RELATED	coord: 1..286
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 1..286
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 159..282 score: 12.68751

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G32160.1	CSPI06G32160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen