CSPI06G30450 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G30450
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	L-ascorbate oxidase
Location	Chr6: 26189485 .. 26191682 (+)
RNA-Seq Expression	CSPI06G30450
Synteny	CSPI06G30450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTCTAAGCCTTTTGGGTTTTAGAAGAAGCACTGAAACGATTCTAACAATGGCTTCGCCGTTACAAAGTACACGATACCTCTTAATTCTTCTCTCCTTCGTCTTCTTCTTACTACAATCTCAAACTGCAGAATCAAGAACTCTGAACTATAAATGGGAAGTGAAATCTGAATTGAAGTCCCCCGATTGCTTTAAAAAAGTTACCTTGACCATTAATGGCCGATCTCCAGGTCCCACTATCTACGCCCACCAAGGTGACACCGTCATCGTTGAGCTCAAGAACAGCCTCTTTACCGAAAACGTGGCTATCCACTGGCATGGAATTCGACAGGTACCCTTAAATTTTCATTTCTCTCCATATTTTTCTTCGTTTGTTTTATAGTTCTTCTTTCTGACGTGTTCGTACATTTGAAGATCGGAACACCATGGAGCGATGGGACAGAAGGTGTCACTCAATGCCCTATTTTGCCTGGTGAAACCTTCAGATATCAGTTTGTTGTAGACAGAGTAAGAGATATTGCACTTACTCGTTTTGTTTTGTCTCTCTCAAAAACTCCATGCTTTAATTTGAGTTTGACCTTTGTGAAATTTGAAATCTGCAGCCAGGAACTTATCTATACCATGCCCATTATGGTATGCAGAGAGAAGATGGTCTCTATGGATCCATTGTGGTTTGGGTTCCAGAAGGACAAACAGAGCCTTTTTCCTATGATCTTGATCGTAACCTCATCCTCACAGATTGGTATCATAAAAGCAGCCACGAACACGCCACTGGATTGGCTACCCCTGGCTCTGGATTCAACTGGGTGGGAGAGCCTGATTCACTGTTGATTCAAGGAAGAGGGAGATTCAACTGCTCTGTTCTGGGATCTTCCAGTGGTACCTGCAATTCATCCAGCACAGAATGTGTTCTTCATCCATTAACAGTCGTACCGGGAAAAACTTACCGATTAAGGGTCTCCAGTCTCACTTCTCTTTCAGCTCTCAGTTTCCAAATTGAGGTAATTACAAGTTCATCTACTTAATTTCATGTACTCTCTTTGAAATTCTTCTTAAATGGCTTAAACTGATAAACTCTCTGTTTTGGAAACAGGGTCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTTCAAGTAAACAATTTGTTCATTTACTCAGGAGAAACATATTCCGTTTTGATAAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTGAGCCGATCAAGAACCACCCCCCCTGGATTAGCCATTTTCAATTACTACCCAAATCACCAGAGTAGACTCCCTCCGACAACTCCTCCAGCACCACCTGCTTGGAACGATGTTACTCCCCGATTGGCTCAAAGTCAAGCCATCAAAGCCCGGCAAGGTTTTATCCACACGCCGCCCAAAGCCGCCGATAAAGTGATCGTGCTTCTCAACACCCAAAACACCGTGAATGGCCGTCGCCGGTGGTCGTTAAATAACGTTTCCTTCAATATGCCTCATACCCCATATCTTATTGCTCTAAAGCACAATCTTCTCCATACATTCAGCCAGGAAAAACCACCCACTGGATACGATTTCAAGAACTACGACATTTTTAATCCGGCGCCCAACCCTGAAGCGACGGTAAGCGACGCCATATACCGGTTGGACTTCAATTCAACTGTGGACATCATACTACAGAACGCCAATATGATGAGCCCAAACAACAGCGAAACACATCCTTGGCATTTGCACGGACACGATTTCTGGGTTTTGGGGTACGGAGAAGGGAAATTCGATCTTTATAAGGATCCAAGCAAGTACAATTTGGAGAACCCGATCATGAAGAACACGGTGCCGTTGCATCCATATGGATGGACCGCTTTGAGATTTGTGGCGGATAATCCTGGTGTCTGGGCTTTCCACTGTCACATCGACGCGCATTTCTTTATGGGAATGGGTGTAGTTTTTGAAGAAGGGATCGAGAGGGTTGGAAAATTGCCTACTTCCATTATGGGATGCGGGGAGAGCAAGAGGTTCTTGAGACCTTAACTCATTTCCATTCCGATTACTATTCTTTAGAAAAACACAAAGAATATTCTTTTATGTTTAGCCAAATTTTTTGTGCTGATTGGGAGTCACTATGAGTGTATAGGTAAATGTTATTGCTACTTAATAAGAAGAAATCGTAATAGTT

mRNA sequence

ATGATTTCTAAGCCTTTTGGGTTTTAGAAGAAGCACTGAAACGATTCTAACAATGGCTTCGCCGTTACAAAGTACACGATACCTCTTAATTCTTCTCTCCTTCGTCTTCTTCTTACTACAATCTCAAACTGCAGAATCAAGAACTCTGAACTATAAATGGGAAGTGAAATCTGAATTGAAGTCCCCCGATTGCTTTAAAAAAGTTACCTTGACCATTAATGGCCGATCTCCAGGTCCCACTATCTACGCCCACCAAGGTGACACCGTCATCGTTGAGCTCAAGAACAGCCTCTTTACCGAAAACGTGGCTATCCACTGGCATGGAATTCGACAGATCGGAACACCATGGAGCGATGGGACAGAAGGTGTCACTCAATGCCCTATTTTGCCTGGTGAAACCTTCAGATATCAGTTTGTTGTAGACAGACCAGGAACTTATCTATACCATGCCCATTATGGTATGCAGAGAGAAGATGGTCTCTATGGATCCATTGTGGTTTGGGTTCCAGAAGGACAAACAGAGCCTTTTTCCTATGATCTTGATCGTAACCTCATCCTCACAGATTGGTATCATAAAAGCAGCCACGAACACGCCACTGGATTGGCTACCCCTGGCTCTGGATTCAACTGGGTGGGAGAGCCTGATTCACTGTTGATTCAAGGAAGAGGGAGATTCAACTGCTCTGTTCTGGGATCTTCCAGTGGTACCTGCAATTCATCCAGCACAGAATGTGTTCTTCATCCATTAACAGTCGTACCGGGAAAAACTTACCGATTAAGGGTCTCCAGTCTCACTTCTCTTTCAGCTCTCAGTTTCCAAATTGAGGGTCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTTCAAGTAAACAATTTGTTCATTTACTCAGGAGAAACATATTCCGTTTTGATAAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTGAGCCGATCAAGAACCACCCCCCCTGGATTAGCCATTTTCAATTACTACCCAAATCACCAGAGTAGACTCCCTCCGACAACTCCTCCAGCACCACCTGCTTGGAACGATGTTACTCCCCGATTGGCTCAAAGTCAAGCCATCAAAGCCCGGCAAGGTTTTATCCACACGCCGCCCAAAGCCGCCGATAAAGTGATCGTGCTTCTCAACACCCAAAACACCGTGAATGGCCGTCGCCGGTGGTCGTTAAATAACGTTTCCTTCAATATGCCTCATACCCCATATCTTATTGCTCTAAAGCACAATCTTCTCCATACATTCAGCCAGGAAAAACCACCCACTGGATACGATTTCAAGAACTACGACATTTTTAATCCGGCGCCCAACCCTGAAGCGACGGTAAGCGACGCCATATACCGGTTGGACTTCAATTCAACTGTGGACATCATACTACAGAACGCCAATATGATGAGCCCAAACAACAGCGAAACACATCCTTGGCATTTGCACGGACACGATTTCTGGGTTTTGGGGTACGGAGAAGGGAAATTCGATCTTTATAAGGATCCAAGCAAGTACAATTTGGAGAACCCGATCATGAAGAACACGGTGCCGTTGCATCCATATGGATGGACCGCTTTGAGATTTGTGGCGGATAATCCTGGTGTCTGGGCTTTCCACTGTCACATCGACGCGCATTTCTTTATGGGAATGGGTGTAGTTTTTGAAGAAGGGATCGAGAGGGTTGGAAAATTGCCTACTTCCATTATGGGATGCGGGGAGAGCAAGAGGTTCTTGAGACCTTAACTCATTTCCATTCCGATTACTATTCTTTAGAAAAACACAAAGAATATTCTTTTATGTTTAGCCAAATTTTTTGTGCTGATTGGGAGTCACTATGAGTGTATAGGTAAATGTTATTGCTACTTAATAAGAAGAAATCGTAATAGTT

Coding sequence (CDS)

ATGGCTTCGCCGTTACAAAGTACACGATACCTCTTAATTCTTCTCTCCTTCGTCTTCTTCTTACTACAATCTCAAACTGCAGAATCAAGAACTCTGAACTATAAATGGGAAGTGAAATCTGAATTGAAGTCCCCCGATTGCTTTAAAAAAGTTACCTTGACCATTAATGGCCGATCTCCAGGTCCCACTATCTACGCCCACCAAGGTGACACCGTCATCGTTGAGCTCAAGAACAGCCTCTTTACCGAAAACGTGGCTATCCACTGGCATGGAATTCGACAGATCGGAACACCATGGAGCGATGGGACAGAAGGTGTCACTCAATGCCCTATTTTGCCTGGTGAAACCTTCAGATATCAGTTTGTTGTAGACAGACCAGGAACTTATCTATACCATGCCCATTATGGTATGCAGAGAGAAGATGGTCTCTATGGATCCATTGTGGTTTGGGTTCCAGAAGGACAAACAGAGCCTTTTTCCTATGATCTTGATCGTAACCTCATCCTCACAGATTGGTATCATAAAAGCAGCCACGAACACGCCACTGGATTGGCTACCCCTGGCTCTGGATTCAACTGGGTGGGAGAGCCTGATTCACTGTTGATTCAAGGAAGAGGGAGATTCAACTGCTCTGTTCTGGGATCTTCCAGTGGTACCTGCAATTCATCCAGCACAGAATGTGTTCTTCATCCATTAACAGTCGTACCGGGAAAAACTTACCGATTAAGGGTCTCCAGTCTCACTTCTCTTTCAGCTCTCAGTTTCCAAATTGAGGGTCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTTCAAGTAAACAATTTGTTCATTTACTCAGGAGAAACATATTCCGTTTTGATAAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTGAGCCGATCAAGAACCACCCCCCCTGGATTAGCCATTTTCAATTACTACCCAAATCACCAGAGTAGACTCCCTCCGACAACTCCTCCAGCACCACCTGCTTGGAACGATGTTACTCCCCGATTGGCTCAAAGTCAAGCCATCAAAGCCCGGCAAGGTTTTATCCACACGCCGCCCAAAGCCGCCGATAAAGTGATCGTGCTTCTCAACACCCAAAACACCGTGAATGGCCGTCGCCGGTGGTCGTTAAATAACGTTTCCTTCAATATGCCTCATACCCCATATCTTATTGCTCTAAAGCACAATCTTCTCCATACATTCAGCCAGGAAAAACCACCCACTGGATACGATTTCAAGAACTACGACATTTTTAATCCGGCGCCCAACCCTGAAGCGACGGTAAGCGACGCCATATACCGGTTGGACTTCAATTCAACTGTGGACATCATACTACAGAACGCCAATATGATGAGCCCAAACAACAGCGAAACACATCCTTGGCATTTGCACGGACACGATTTCTGGGTTTTGGGGTACGGAGAAGGGAAATTCGATCTTTATAAGGATCCAAGCAAGTACAATTTGGAGAACCCGATCATGAAGAACACGGTGCCGTTGCATCCATATGGATGGACCGCTTTGAGATTTGTGGCGGATAATCCTGGTGTCTGGGCTTTCCACTGTCACATCGACGCGCATTTCTTTATGGGAATGGGTGTAGTTTTTGAAGAAGGGATCGAGAGGGTTGGAAAATTGCCTACTTCCATTATGGGATGCGGGGAGAGCAAGAGGTTCTTGAGACCTTAA

Protein sequence

MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP*

Homology

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BLAST of CSPI06G30450 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 3.5e-175
Identity = 291/565 (51.50%), Postives = 381/565 (67.43%), Query Frame = 0


Query: 10  YLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQG 69
           +L++ + F F +  S+    +  +YKW+V+    SPDC + + + ING  PGPTI A+ G
Sbjct: 17  FLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEFPGPTIRANAG 76

Query: 70  DTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTY 129
           D V+VEL N L TE V IHWHGI Q GTPW+DGT  ++QC I PGETF Y+FVVD+ GTY
Sbjct: 77  DIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVDKAGTY 136

Query: 130 LYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGS 189
            YH H GMQR  GLYGS++V  PEG++EPF YD + NL+L+DW+H+S H+   GL++   
Sbjct: 137 FYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHKQEVGLSS--K 196

Query: 190 GFNWVGEPDSLLIQGRGRFNCSVLGSSS---GTCNSSSTE-CVLHPLTVVPGKTYRLRVS 249
              W+GEP S+LI G+G+F+CS+    +     C  S  E C    L V P KTYR+R++
Sbjct: 197 PMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPKKTYRIRIA 256

Query: 250 SLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWIT 309
           S T+L++L+F I  H + VVEADG+YV+PF  +++ IYSGE+YSVLI  DQ+P  NYW++
Sbjct: 257 STTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQNPLENYWVS 316

Query: 310 TNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHT 369
             V +R   TPPGL + NY PN  S+LP + PP  P W D       +  I A  G    
Sbjct: 317 IGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIFAAMG-SPK 376

Query: 370 PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYD 429
           PP   ++ + LLNTQN +NG  +W++NNVS  +P TPYL A+K  L   F+Q  PP  + 
Sbjct: 377 PPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQNPPPETFP 436

Query: 430 FKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVL 489
             NYDI NP PNPE T  + +Y+ +   TVD+ILQNANM++PN SE HPWHLHGHDFWVL
Sbjct: 437 L-NYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHGHDFWVL 496

Query: 490 GYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMG 549
           GYGEGKF   +D  K NL+NP ++NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MG
Sbjct: 497 GYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMG 556

Query: 550 MGVVFEEGIERVGKLPTSIMGCGES 571
           MGVVF EG+  VG +P   + CG +
Sbjct: 557 MGVVFAEGVHMVGMIPPKALACGST 577

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BLAST of CSPI06G30450 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 609.8 bits (1571), Expect = 3.3e-173
Identity = 290/568 (51.06%), Postives = 385/568 (67.78%), Query Frame = 0


Query: 12  LILLSFVFFLLQSQTAE-SRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGD 71
           LIL  F   L    ++E S+  +YKWEV+    +PDC + + + ING+ PGPTI A+ GD
Sbjct: 13  LILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRANAGD 72

Query: 72  TVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYL 131
           TV+VEL N L TE V IHWHGI Q GTPW+DGT  ++QC I PGETF Y F VD PGT+ 
Sbjct: 73  TVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFF 132

Query: 132 YHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSG 191
           YH H GMQR  GLYGS++V  P+G+ EPF YD + NL+L+DW+H+S H+   GL++    
Sbjct: 133 YHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS--KP 192

Query: 192 FNWVGEPDSLLIQGRGRFNCSVLG---SSSGTCNSSSTE-CVLHPLTVVPGKTYRLRVSS 251
             W+GEP ++L+ GRG+F+CS+     S+   C    +E C  +   V+P KTYR+R++S
Sbjct: 193 IRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRIAS 252

Query: 252 LTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITT 311
            T+L+AL+F I  H + VVEADG+YV+PF  +++ IYSGE+YSVLI  DQ+PS NYW++ 
Sbjct: 253 TTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSV 312

Query: 312 NVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTP 371
               R   TPPGL + NY PN  S+LP + PP  PAW+D       +  I A  G    P
Sbjct: 313 GTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMG-SPKP 372

Query: 372 PKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDF 431
           P  +++ I LLNTQN +NG  +W++N+VS  +P TPYL A+K NLLH F Q  PP  +  
Sbjct: 373 PVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVFP- 432

Query: 432 KNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLG 491
           ++YDI  P  N +  + + +Y+      VD+ILQNANMM  N SE HPWHLHGHDFWVLG
Sbjct: 433 EDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLG 492

Query: 492 YGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGM 551
           YG+GKF   ++ S  NL+NP ++NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGM
Sbjct: 493 YGDGKFTA-EEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGM 552

Query: 552 GVVFEEGIERVGKLPTSIMGCGESKRFL 575
           GVVF EG+E+VG++PT  + CG + + L
Sbjct: 553 GVVFAEGVEKVGRIPTKALACGGTAKSL 575

[Close]

BLAST of CSPI06G30450 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 3.3e-173
Identity = 283/550 (51.45%), Postives = 378/550 (68.73%), Query Frame = 0


Query: 29  SRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAIH 88
           S+  +YKWEV+    +P+C + + + ING+ PGPTI A+ GD+V+VEL N L TE V IH
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 89  WHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIV 148
           WHGI Q GTPW+DGT  ++QC I PGETF Y F VD PGT+ YH H GMQR  GLYGS++
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 149 VWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRF 208
           V  P+G+ EPF YD + NL+L+DW+H+S H+   GL++      W+GEP ++L+ GRG+F
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS--KPIRWIGEPQTILLNGRGQF 180

Query: 209 NCSVLG---SSSGTCNSSSTE-CVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTV 268
           +CS+     S+   C    +E C  +   V P KTYR+R++S T+L+AL+F I  H + V
Sbjct: 181 DCSIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLV 240

Query: 269 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 328
           VEADG+YV+PF  +++ IYSGE+YSVLI  DQ+PS NYW++    +R   TPPGL + NY
Sbjct: 241 VEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNY 300

Query: 329 YPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVN 388
            PN  S+LP + PP  PAW+D       +  I A  G    PP   ++ I LLNTQN +N
Sbjct: 301 LPNSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMG-SPKPPVKFNRRIFLLNTQNVIN 360

Query: 389 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 448
           G  +W++N+VS  +P TPYL A+K+NLLH F Q  PP  +  ++YDI  P  N +  + +
Sbjct: 361 GYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFP-EDYDIDTPPTNEKTRIGN 420

Query: 449 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLE 508
            +Y+      VD+ILQNANMM  N SETHPWHLHGHDFWVLGYG+GKF   ++ S  NL+
Sbjct: 421 GVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSA-EEESSLNLK 480

Query: 509 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 568
           NP ++NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGMGVVF EG+E+VG++PT  
Sbjct: 481 NPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKA 540

Query: 569 MGCGESKRFL 575
           + CG + + L
Sbjct: 541 LACGGTAKSL 545

[Close]

BLAST of CSPI06G30450 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 1.4e-171
Identity = 283/556 (50.90%), Postives = 372/556 (66.91%), Query Frame = 0


Query: 24  SQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTE 83
           + TA +    Y WEV+ +   PDC + + + +NG+ PGPTI+A  GDT++V L N L TE
Sbjct: 13  AHTASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATE 72

Query: 84  NVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGL 143
            + IHWHGIRQ+G+PW+DG  GVTQC I PGETF Y F VD+PGT+ YH HYGMQR  GL
Sbjct: 73  GLVIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAGL 132

Query: 144 YGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQ 203
           YGS+++ V +G+ EP  YD + NL+L+DW+H+       GL++      W+GE  S+LI 
Sbjct: 133 YGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSS--RPMRWIGEAQSILIN 192

Query: 204 GRGRFNCSVLGSSSGTCNSSST-----ECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIE 263
           GRG+FNCS+    S T   + T     +C    L V P KTYR+R++S T+L++L+F ++
Sbjct: 193 GRGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLNFAVQ 252

Query: 264 GHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPG 323
           GH + VVEADG+Y+ PF  +++ IYSGETYSVL+  DQDPS+NY+IT  V  R   TPP 
Sbjct: 253 GHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKPNTPPA 312

Query: 324 LAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLN 383
           L + NY     S+LP + PP  P WND       S+ I A  G   +PP+  D+ ++LLN
Sbjct: 313 LTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMG-SPSPPETFDERLILLN 372

Query: 384 TQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNP 443
           TQN + G  +W++NNVS  +P TPYL ++K+NL   F++  PP  Y   +YDI  P  N 
Sbjct: 373 TQNLIEGFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPV-DYDIMTPPRNR 432

Query: 444 EATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDP 503
            A   +      FN TVD+ILQNAN ++ N SE HPWHLHGHDFWVLGYGEGKF    D 
Sbjct: 433 NAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKPGVDE 492

Query: 504 SKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVG 563
             YNL+NP ++NTV L+PYGWTALRFV DNPGVW FHCHI+ H  MGMGVVF EG+ R+G
Sbjct: 493 KTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIG 552

Query: 564 KLPTSIMGCGESKRFL 575
           K+P   +GCG +K+FL
Sbjct: 553 KVPDEALGCGLTKQFL 564

[Close]

BLAST of CSPI06G30450 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 3.2e-168
Identity = 292/578 (50.52%), Postives = 380/578 (65.74%), Query Frame = 0


Query: 15  LSFVFF------LLQSQTAES----RTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTI 74
           L F+FF      LL+  ++ S    +T ++KW+V+    SPD  + V + ING+ PGPTI
Sbjct: 4   LGFLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTI 63

Query: 75  YAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVD 134
            A  GDTV V L N L TE V IHWHGIRQIGTPW+DGT  ++QC I PGETF Y+F VD
Sbjct: 64  RAKAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVD 123

Query: 135 RPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGL 194
           + GTY YH HYGMQR  GLYGS++V V EG+ EPF YD + NL+L+DW+HK SHE    L
Sbjct: 124 KAGTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDL 183

Query: 195 ATPGSGFNWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLHP--------LTVVP 254
           ++  +   W+GEP +LL+ GRG++NCS+    S        +C L          L V P
Sbjct: 184 SS--NPLRWIGEPQTLLLNGRGQYNCSLAARFS---KPPLPQCKLRGGEQYAPQILRVRP 243

Query: 255 GKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQ 314
            K YRLRV+S T+L +LS  I GH M VVEADG+YV+PF V ++ IYSGE+YSVL K DQ
Sbjct: 244 NKIYRLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQ 303

Query: 315 DPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAI 374
           DP++NYWI+ NV  R   TP GL + NY PN  S+ P   PP  P WND     + S  I
Sbjct: 304 DPTKNYWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKI 363

Query: 375 KARQGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFS 434
            A  G    PP+   + I+LLNTQN ++G  +W++NNVS  +P   YL ++++  ++ F 
Sbjct: 364 FALMGSPKPPPQ-NHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYG-INAFD 423

Query: 435 QEKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWH 494
            + PP  +  K+YD+   APN  +T  + +Y L FN+T+DIILQNAN ++ + SE HPWH
Sbjct: 424 TKPPPDNFP-KDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWH 483

Query: 495 LHGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHC 554
           LHGHDFWVLGYGEGKF   KD  K+NL+NP ++NT  + P+GWTALRFV DNPGVWAFHC
Sbjct: 484 LHGHDFWVLGYGEGKFS-EKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHC 543

Query: 555 HIDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFL 575
           HI+ H  MGMGV+F EG+  V K+P   + CG + + L
Sbjct: 544 HIEPHLHMGMGVIFAEGVHLVKKIPKEALACGLTGKML 572

[Close]

BLAST of CSPI06G30450 vs. ExPASy TrEMBL
Match: A0A0A0KK62 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1)

HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 575/576 (99.83%), Postives = 576/576 (100.00%), Query Frame = 0


Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLHPLTVVPGKTY 240
           ATGLATPGSGFNWVGEPDSLLIQGRGRFNCS+LGSSSGTCNSSSTECVLHPLTVVPGKTY
Sbjct: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360

Query: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of CSPI06G30450 vs. ExPASy TrEMBL
Match: A0A5A7SYW4 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002620 PE=3 SV=1)

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 550/576 (95.49%), Postives = 561/576 (97.40%), Query Frame = 0


Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           MAS LQ  +Y L+LLS   FLLQSQTAESRTLNYKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYD DRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLHPLTVVPGKTY 240
           A GLATPGSGF WVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVL+PLTVVPGKTY
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQS+ PPTTPPAPPAWNDV PRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360

Query: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAI+RLDFNSTVD+ILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD+Y DPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of CSPI06G30450 vs. ExPASy TrEMBL
Match: A0A1S3B151 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1)

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 550/576 (95.49%), Postives = 561/576 (97.40%), Query Frame = 0


Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           MAS LQ  +Y L+LLS   FLLQSQTAESRTLNYKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYD DRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLHPLTVVPGKTY 240
           A GLATPGSGF WVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVL+PLTVVPGKTY
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQS+ PPTTPPAPPAWNDV PRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360

Query: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAI+RLDFNSTVD+ILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD+Y DPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of CSPI06G30450 vs. ExPASy TrEMBL
Match: A0A6J1KU77 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111497239 PE=3 SV=1)

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 512/576 (88.89%), Postives = 541/576 (93.92%), Query Frame = 0


Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           M S LQ  +YLLILL F  FL +S+TAESRTL Y+WEVKSELKSPDCFKK+TLTINGRSP
Sbjct: 1   MGSLLQRRQYLLILLCFFVFLWESRTAESRTLYYRWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPF+YD DRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLHPLTVVPGKTY 240
           + GLA PGS FNWVGEPDSLLIQGRGRFNCS+   +S TCNSSS +CVL+P+TVVPG+TY
Sbjct: 181 SAGLAAPGSAFNWVGEPDSLLIQGRGRFNCSL--GASATCNSSSPDCVLYPITVVPGRTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+PSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360
           NYWITTNVVSR+ TTP GLAIFNYYPNHQ + PPTTPPA PAWN V PRLAQS AIKARQ
Sbjct: 301 NYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQ 360

Query: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ KP
Sbjct: 361 GFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNY+IF+P PNPEATVSDAIYRLDFNSTVDIILQNANMM+ N SETHPWHLHGH
Sbjct: 421 PTGYDFKNYNIFDPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD++KDPSKYNL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIE+V KLPTSIMGCGESKRF+RP
Sbjct: 541 HFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 574

[Close]

BLAST of CSPI06G30450 vs. ExPASy TrEMBL
Match: A0A6J1GE53 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111453168 PE=3 SV=1)

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 511/579 (88.26%), Postives = 536/579 (92.57%), Query Frame = 0


Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           MA  LQST+YLLIL  F  FL  S  AESRTL YKWEVK E++SPDCFKK+T+TING SP
Sbjct: 1   MAPLLQSTQYLLILFCFFVFLSASLVAESRTLFYKWEVKYEIRSPDCFKKLTITINGGSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGD VIVELKNSL +ENVA+HWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDNVIVELKNSLASENVAVHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDR GTYLYHAHYGMQR+DGLYGSIVVW+PEGQ+EPFSYD DRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRAGTYLYHAHYGMQRQDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSVLGSS---SGTCNSSSTECVLHPLTVVPG 240
           ATGLATP  GF WVGEPDSLLIQGRGRF CS+LGSS   S TCNSSS +CV +PLTVVPG
Sbjct: 181 ATGLATPDPGFQWVGEPDSLLIQGRGRFKCSLLGSSGLASATCNSSSPQCVAYPLTVVPG 240

Query: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300
           KTYRLRVSSLTSLSALSFQIE HTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIESHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 301 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIK 360
           PSRNYW TTNVVSR RTTPPGLAIFNYYPNH+ +LPPTTPPA PAWNDV PRLAQS AIK
Sbjct: 301 PSRNYWATTNVVSRPRTTPPGLAIFNYYPNHRRKLPPTTPPAGPAWNDVAPRLAQSHAIK 360

Query: 361 ARQGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420
           ARQGFIH PPK ADKVIVLLNTQNTV GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ
Sbjct: 361 ARQGFIHPPPKIADKVIVLLNTQNTVKGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHL 480
            KPPTGYDFKNYDIFNPAPN EAT SDAI+RL+F STVDIILQNANMMSPN+S+THPWHL
Sbjct: 421 AKPPTGYDFKNYDIFNPAPNQEATESDAIFRLEFKSTVDIILQNANMMSPNSSDTHPWHL 480

Query: 481 HGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540
           HGHDFWVLGYGEGKFD+Y+DPSKYNLENPIMKNTV LHPYGWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDIYRDPSKYNLENPIMKNTVALHPYGWTALRFVADNPGVWAFHCH 540

Query: 541 IDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           IDAHFFMGMGVVFEEGI++VG LPTSIMGCGESKRFLRP
Sbjct: 541 IDAHFFMGMGVVFEEGIDQVGNLPTSIMGCGESKRFLRP 579

[Close]

BLAST of CSPI06G30450 vs. NCBI nr
Match: XP_004143482.1 (L-ascorbate oxidase [Cucumis sativus] >KGN48772.1 hypothetical protein Csa_003073 [Cucumis sativus])

HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 575/576 (99.83%), Postives = 576/576 (100.00%), Query Frame = 0


Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLHPLTVVPGKTY 240
           ATGLATPGSGFNWVGEPDSLLIQGRGRFNCS+LGSSSGTCNSSSTECVLHPLTVVPGKTY
Sbjct: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360

Query: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of CSPI06G30450 vs. NCBI nr
Match: XP_008440636.1 (PREDICTED: L-ascorbate oxidase [Cucumis melo] >KAA0036280.1 L-ascorbate oxidase [Cucumis melo var. makuwa] >TYK12674.1 L-ascorbate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 550/576 (95.49%), Postives = 561/576 (97.40%), Query Frame = 0


Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           MAS LQ  +Y L+LLS   FLLQSQTAESRTLNYKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYD DRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLHPLTVVPGKTY 240
           A GLATPGSGF WVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVL+PLTVVPGKTY
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQS+ PPTTPPAPPAWNDV PRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360

Query: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAI+RLDFNSTVD+ILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD+Y DPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of CSPI06G30450 vs. NCBI nr
Match: XP_038881120.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 1145.6 bits (2962), Expect = 0.0e+00
Identity = 538/577 (93.24%), Postives = 556/577 (96.36%), Query Frame = 0


Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           MAS LQS +YLLILL F+ FL+QS  AESRTLNYKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 18  MASLLQSRQYLLILLCFLLFLIQSPIAESRTLNYKWEVKSELKSPDCFKKLTMTINGRSP 77

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+YQ
Sbjct: 78  GPTIYAHQGDTVIVELKNSFSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPFSYD DRNLILTDWYHK+SHEH
Sbjct: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHKTSHEH 197

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSVLGSS-SGTCNSSSTECVLHPLTVVPGKT 240
           A GLATPGSGF WVGEPDSLLIQGRGRFNCSVLGSS S TCNSS+ EC+L+P+TVVPGKT
Sbjct: 198 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSTSATCNSSNPECILYPITVVPGKT 257

Query: 241 YRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPS 300
           YRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPS
Sbjct: 258 YRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPS 317

Query: 301 RNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKAR 360
           RNYWITTNVVSRSRTTPPGLAIFNYYPNHQ +LPPTTPPAPPAWNDV PRLAQS AIKAR
Sbjct: 318 RNYWITTNVVSRSRTTPPGLAIFNYYPNHQRKLPPTTPPAPPAWNDVAPRLAQSHAIKAR 377

Query: 361 QGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEK 420
           QGFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLH FSQ+K
Sbjct: 378 QGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHAFSQDK 437

Query: 421 PPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHG 480
           PPTGYDFKNYDIFNPAPNPEATVSDAI+RLDFNSTVDIILQNANMMSPNNSETHPWHLHG
Sbjct: 438 PPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHG 497

Query: 481 HDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHID 540
           HDFWVLGYGEGKFD+YKDPSK+NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHID
Sbjct: 498 HDFWVLGYGEGKFDIYKDPSKFNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHID 557

Query: 541 AHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           AHFFMGMGVVF EGI+RV KLPTSIMGCGESKRFLRP
Sbjct: 558 AHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 594

[Close]

BLAST of CSPI06G30450 vs. NCBI nr
Match: KAG6594863.1 (L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826.1 L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 514/576 (89.24%), Postives = 542/576 (94.10%), Query Frame = 0


Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           M S LQS +YLLILL F  FL +S+TAESRTL YKWEVKSELKSPDCFKK+TLTINGRSP
Sbjct: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPF+YD DRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLHPLTVVPGKTY 240
           + GLA  GS F WVGEPDSLLIQGRGRFNCS+LG+S+ TCNSSS +CVL+P+TVVPG+TY
Sbjct: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASA-TCNSSSPDCVLYPITVVPGRTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+PSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360
           NYWITTNVVSR+ TTP GLAIFNYYPNHQ + PPTTPPA PAWN V PRLAQS AIKARQ
Sbjct: 301 NYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQ 360

Query: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ KP
Sbjct: 361 GFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNY+IF P PNPEATVSDAIYRLDFNSTVDIILQNANMM+ N SETHPWHLHGH
Sbjct: 421 PTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD++KDPSKYNL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIE+V KLPTSIMGCGESKRF+RP
Sbjct: 541 HFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 575

[Close]

BLAST of CSPI06G30450 vs. NCBI nr
Match: XP_023003749.1 (L-ascorbate oxidase [Cucurbita maxima])

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 512/576 (88.89%), Postives = 541/576 (93.92%), Query Frame = 0


Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           M S LQ  +YLLILL F  FL +S+TAESRTL Y+WEVKSELKSPDCFKK+TLTINGRSP
Sbjct: 1   MGSLLQRRQYLLILLCFFVFLWESRTAESRTLYYRWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPF+YD DRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLHPLTVVPGKTY 240
           + GLA PGS FNWVGEPDSLLIQGRGRFNCS+   +S TCNSSS +CVL+P+TVVPG+TY
Sbjct: 181 SAGLAAPGSAFNWVGEPDSLLIQGRGRFNCSL--GASATCNSSSPDCVLYPITVVPGRTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+PSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360
           NYWITTNVVSR+ TTP GLAIFNYYPNHQ + PPTTPPA PAWN V PRLAQS AIKARQ
Sbjct: 301 NYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQ 360

Query: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ KP
Sbjct: 361 GFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNY+IF+P PNPEATVSDAIYRLDFNSTVDIILQNANMM+ N SETHPWHLHGH
Sbjct: 421 PTGYDFKNYNIFDPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD++KDPSKYNL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIE+V KLPTSIMGCGESKRF+RP
Sbjct: 541 HFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 574

[Close]

BLAST of CSPI06G30450 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 808.1 bits (2086), Expect = 4.5e-234
Identity = 382/569 (67.14%), Postives = 445/569 (78.21%), Query Frame = 0


Query: 12  LILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDT 71
           L++L F+     S   + +   +KWEVK E KSPDCF+K+ +TING+ PGPTI A QGDT
Sbjct: 16  LMVLCFIALFFSSVLCQGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDT 75

Query: 72  VIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLY 131
           ++VELKNS  TENVA+HWHGIRQIGTPW DG EGVTQCPILPGE F YQFVVDRPGTY+Y
Sbjct: 76  IVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMY 135

Query: 132 HAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGF 191
           H+HYGMQRE GL G I V  P  + EPF+YD DRN +LTDWYHKS  E ATGLA+    F
Sbjct: 136 HSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLAS--IPF 195

Query: 192 NWVGEPDSLLIQGRGRFNCSVLGSS-----SGTCNSSSTECVLHPLTVVPGKTYRLRVSS 251
            WVGEP SL+IQGRGRFNCS   ++     SG CN S+ +C    LTV+PGKTYRLR+ S
Sbjct: 196 KWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRLRIGS 255

Query: 252 LTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITT 311
           LT+LSALSFQIEGH +TVVEADGHYVEPF V NLF+YSGETYSVL+KADQ+P RNYWIT+
Sbjct: 256 LTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITS 315

Query: 312 NVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPA--PPAWNDVTPRLAQSQAIKARQGFIH 371
           ++VSR  TTPP  A+ NYYPNH  R PPT+  +   P WND   RLAQS AIKAR+GFIH
Sbjct: 316 SIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGFIH 375

Query: 372 TPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFS-QEKPPTG 431
             P+ +DKVIVLLNTQN VNG RRWS+NNVS++ P TPYLIALK NL + F  +   P  
Sbjct: 376 ALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPEN 435

Query: 432 YDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFW 491
           YD +NYDIF    N  AT SD IYRL FNSTVD+ILQNAN M+ NNSETHPWHLHGHDFW
Sbjct: 436 YDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFW 495

Query: 492 VLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFF 551
           VLGYGEGKF+  +DP +YN  +PI KNTV + P+GWTALRF ADNPGVW+FHCHI++HFF
Sbjct: 496 VLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFF 555

Query: 552 MGMGVVFEEGIERVGKLPTSIMGCGESKR 573
           MGMG+VFE GI++V  LP+SIMGCG++KR
Sbjct: 556 MGMGIVFESGIDKVSSLPSSIMGCGQTKR 582

[Close]

BLAST of CSPI06G30450 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 591.7 bits (1524), Expect = 6.6e-169
Identity = 277/557 (49.73%), Postives = 368/557 (66.07%), Query Frame = 0


Query: 24  SQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTE 83
           + TA +    Y WEV+ +  SPDC +   +T+NG  PGPTI A  GDT++V L N L TE
Sbjct: 30  THTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTE 89

Query: 84  NVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGL 143
            + IHWHGIRQ G+PW+DG  GVTQC I PGETF Y F V++PGT+ YH HYGMQR  GL
Sbjct: 90  GLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGL 149

Query: 144 YGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQ 203
           YGS++V V +G++E   YD + NL+L+DW+H++      GL++      W+GE  S+LI 
Sbjct: 150 YGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPMRWIGEAQSILIN 209

Query: 204 GRGRFNCSVLGSSSGTCN------SSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQI 263
           GRG+FNCS+    S   +          +C    L V P KTYR+R+SS T+L++L+  +
Sbjct: 210 GRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAV 269

Query: 264 EGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPP 323
           +GH + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+  V  R   T  
Sbjct: 270 QGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQ 329

Query: 324 GLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLL 383
            L I NY     S+LP + PP  P W+D       S+ I +  G   +PPK   K ++LL
Sbjct: 330 ALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRKRLILL 389

Query: 384 NTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPN 443
           NTQN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y   +YDI NP P 
Sbjct: 390 NTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPF 449

Query: 444 PEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKD 503
           P  T  + IY   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF    D
Sbjct: 450 PNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGID 509

Query: 504 PSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERV 563
              YNL+NP ++NT  L+PYGWTA+RFV DNPGVW FHCHI+ H  MGMGVVF EG+ R+
Sbjct: 510 EKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRI 569

Query: 564 GKLPTSIMGCGESKRFL 575
           GK+P   +GCG +K+FL
Sbjct: 570 GKVPDEALGCGLTKQFL 582

[Close]

BLAST of CSPI06G30450 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 570.1 bits (1468), Expect = 2.0e-162
Identity = 273/557 (49.01%), Postives = 360/557 (64.63%), Query Frame = 0


Query: 26  TAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENV 85
           +A +  +   WEV+ +   PDC + + + ING+ PGPTI A  GDTVI+ + N L TE V
Sbjct: 18  SASAAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGV 77

Query: 86  AIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYG 145
            IHWHGIRQ GTPW+DG  GVTQCPI PGETF Y+F+VD+ GT+ YH HYGMQR  GLYG
Sbjct: 78  VIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYG 137

Query: 146 SIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGR 205
            ++V  P+   E   YD + NL+L+DW+H+S   HA  LA       W+GEP SLLI GR
Sbjct: 138 MLIVRSPK---ERLIYDGEFNLLLSDWWHQSI--HAQELALSSRPMRWIGEPQSLLINGR 197

Query: 206 GRFNCSVL-----GSSSGTCN-SSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEG 265
           G+FNCS       G     C    + +C    L V P + YRLR++S T+L++L+  ++G
Sbjct: 198 GQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQG 257

Query: 266 HTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGL 325
           H + VVEADG+YV PF VN++ +YSGETYSVL+K +  PS+ YWI+  V  R   TP  L
Sbjct: 258 HQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQAL 317

Query: 326 AIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNT 385
            + NY    +SR P   PP  P WND     + S+ I A +G+   P K+ D+ ++LLNT
Sbjct: 318 TVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQ-LILLNT 377

Query: 386 QNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPE 445
           QN      +WS+NNVS ++P TPYL ++++ L   +  + P       NYDI  P PNP 
Sbjct: 378 QNLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPN 437

Query: 446 ATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPS 505
            T    IY   F   VD+ILQNAN++    SE HPWH+HGHDFWVLGYGEGKF    D  
Sbjct: 438 TTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEK 497

Query: 506 KYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGK 565
            +NL+NP ++NTV L+P+GWTA+RFV DNPGVW FHCHI+ H  MGMGVVF EG++R+GK
Sbjct: 498 TFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGK 557

Query: 566 --LPTSIMGCGESKRFL 575
             +P   +GCG ++++L
Sbjct: 558 MEIPDEALGCGLTRKWL 567

[Close]

BLAST of CSPI06G30450 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 525.4 bits (1352), Expect = 5.8e-149
Identity = 250/513 (48.73%), Postives = 334/513 (65.11%), Query Frame = 0


Query: 24  SQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTE 83
           + TA +    Y WEV+ +  SPDC +   +T+NG  PGPTI A  GDT++V L N L TE
Sbjct: 30  THTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTE 89

Query: 84  NVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGL 143
            + IHWHGIRQ G+PW+DG  GVTQC I PGETF Y F V++PGT+ YH HYGMQR  GL
Sbjct: 90  GLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGL 149

Query: 144 YGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQ 203
           YGS++V V +G++E   YD + NL+L+DW+H++      GL++      W+GE  S+LI 
Sbjct: 150 YGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPMRWIGEAQSILIN 209

Query: 204 GRGRFNCSVLGSSSGTCN------SSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQI 263
           GRG+FNCS+    S   +          +C    L V P KTYR+R+SS T+L++L+  +
Sbjct: 210 GRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAV 269

Query: 264 EGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPP 323
           +GH + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+  V  R   T  
Sbjct: 270 QGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQ 329

Query: 324 GLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLL 383
            L I NY     S+LP + PP  P W+D       S+ I +  G   +PPK   K ++LL
Sbjct: 330 ALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRKRLILL 389

Query: 384 NTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPN 443
           NTQN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y   +YDI NP P 
Sbjct: 390 NTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPF 449

Query: 444 PEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKD 503
           P  T  + IY   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF    D
Sbjct: 450 PNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGID 509

Query: 504 PSKYNLENPIMKNTVPLHPYGWTALRFVADNPG 531
              YNL+NP ++NT  L+PYGWTA+RFV DNPG
Sbjct: 510 EKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

[Close]

BLAST of CSPI06G30450 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 391.7 bits (1005), Expect = 1.0e-108
Identity = 189/382 (49.48%), Postives = 251/382 (65.71%), Query Frame = 0


Query: 199 SLLIQGRGRFNCSVLGSSSGTCN------SSSTECVLHPLTVVPGKTYRLRVSSLTSLSA 258
           S+LI GRG+FNCS+    S   +          +C    L V P KTYR+R+SS T+L++
Sbjct: 12  SILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALAS 71

Query: 259 LSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRS 318
           L+  ++GH + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+  V  R 
Sbjct: 72  LNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRK 131

Query: 319 RTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADK 378
             T   L I NY     S+LP + PP  P W+D       S+ I +  G   +PPK   K
Sbjct: 132 PNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRK 191

Query: 379 VIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIF 438
            ++LLNTQN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y   +YDI 
Sbjct: 192 RLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIM 251

Query: 439 NPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKF 498
           NP P P  T  + IY   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF
Sbjct: 252 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 311

Query: 499 DLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEE 558
               D   YNL+NP ++NT  L+PYGWTA+RFV DNPGVW FHCHI+ H  MGMGVVF E
Sbjct: 312 KPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 371

Query: 559 GIERVGKLPTSIMGCGESKRFL 575
           G+ R+GK+P   +GCG +K+FL
Sbjct: 372 GLNRIGKVPDEALGCGLTKQFL 391

The following BLAST results are available for this feature:

BLAST of CSPI06G30450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P14133	3.5e-175	51.50	L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P24792	3.3e-173	51.06	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2	[more]
P37064	3.3e-173	51.45	L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1	[more]
M4DUF2	1.4e-171	50.90	L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1	[more]
Q40588	3.2e-168	50.52	L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1	[more]

BLAST of CSPI06G30450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KK62	0.0e+00	99.83	L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1	[more]
A0A5A7SYW4	0.0e+00	95.49	L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G...	[more]
A0A1S3B151	0.0e+00	95.49	L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1	[more]
A0A6J1KU77	0.0e+00	88.89	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111497239 PE=3 SV=1	[more]
A0A6J1GE53	0.0e+00	88.26	L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111453168 PE=3 SV=1	[more]

BLAST of CSPI06G30450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143482.1	0.0e+00	99.83	L-ascorbate oxidase [Cucumis sativus] >KGN48772.1 hypothetical protein Csa_00307...	[more]
XP_008440636.1	0.0e+00	95.49	PREDICTED: L-ascorbate oxidase [Cucumis melo] >KAA0036280.1 L-ascorbate oxidase ...	[more]
XP_038881120.1	0.0e+00	93.24	L-ascorbate oxidase [Benincasa hispida]	[more]
KAG6594863.1	0.0e+00	89.24	L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826...	[more]
XP_023003749.1	0.0e+00	88.89	L-ascorbate oxidase [Cucurbita maxima]	[more]

BLAST of CSPI06G30450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39830.1	4.5e-234	67.14	Cupredoxin superfamily protein	[more]
AT5G21105.1	6.6e-169	49.73	Plant L-ascorbate oxidase	[more]
AT5G21100.1	2.0e-162	49.01	Plant L-ascorbate oxidase	[more]
AT5G21105.3	5.8e-149	48.73	Plant L-ascorbate oxidase	[more]
AT5G21105.2	1.0e-108	49.48	Plant L-ascorbate oxidase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 364..574 e-value: 8.0E-70 score: 236.8
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 20..152 e-value: 6.3E-47 score: 161.5
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 159..362 e-value: 5.4E-62 score: 211.5
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 158..348
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 20..178
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 367..569
IPR011706	Multicopper oxidase, C-terminal	PFAM	PF07731	Cu-oxidase_2	coord: 431..553 e-value: 3.2E-37 score: 127.3
IPR017760	L-ascorbate oxidase, plants	TIGRFAM	TIGR03388	TIGR03388	coord: 33..569 e-value: 6.4E-282 score: 934.0
IPR001117	Multicopper oxidase, type 1	PFAM	PF00394	Cu-oxidase	coord: 167..326 e-value: 2.2E-47 score: 161.1
IPR011707	Multicopper oxidase, N-termianl	PFAM	PF07732	Cu-oxidase_3	coord: 41..150 e-value: 1.8E-38 score: 131.2
None	No IPR available	PANTHER	PTHR11709:SF218	OS09G0365900 PROTEIN	coord: 15..570
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 15..570
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 535..546
IPR033138	Multicopper oxidases, conserved site	PROSITE	PS00079	MULTICOPPER_OXIDASE1	coord: 530..550
IPR034267	Ascorbate oxidase, third cupredoxin domain	CDD	cd13893	CuRO_3_AAO	coord: 370..561 e-value: 6.75023E-85 score: 259.274

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G30450.1	CSPI06G30450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1900367	positive regulation of defense response to insect
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0008447	L-ascorbate oxidase activity
molecular_function	GO:0016491	oxidoreductase activity