CSPI06G28970 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G28970
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionNADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
LocationChr6: 25208957 .. 25212582 (+)
RNA-Seq ExpressionCSPI06G28970
SyntenyCSPI06G28970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCAGCCAAATGAGTTACGTTCGGCCTCTCTCTTATTTGATGTTTTTCGTATTATTTTAAAGGCCAACCTCCATTTTCGACAAGTTTTAAACAAAACAACACCTTAAAATAAATCTCTTCTCCGTCGTTCAAAGTTCAATCGAGTTGGAAGTTTGTTTGTAACGGGGGACGAGGCTGACGGAAAACTAGTTTTCGCGTCGAATTTTCTTCATCCACGGATGGCTAACTCCTTGCAGCGAATCTGGAGCCACGGACGTGCTCTTCGGGGTACTCTGGCGCCTCTTTCCAGGTCATTCTCCACCGATTCCTTGGTTGAAGTGAAGCCTGGCGAGATCGGTATAGTCTCTGGAATTCCAGAGGAGCATCTTACTAGAAGGGTATAGCCCAGATATTTCTTTTTCTTTACTTTTTATCAACGAGTTTCCAATTGCTTACATTTTAGTTTAATTTCGAGGGATCGGGTTATTGGATGTTAGTGATGTTTTTTGGGATTAGGTTTTTTTATACCCATCTGCTTGGGTGTTCTTTCTTGTTTATAACTTGGCCTTATTAGAATGGTTTGCAGTGGCTAAACCCCCTTAGGTGTTACTTTGTTACTAGTATGTTGAAATGTAGTCGGTTCCCATGAGGAGATGGAAATGTCCGGCTGTTCATAACCAAAAATTGATTAAAATGAGGTTAAAGTATGTCGAGGAGCCCGAGTTCTCTTCTTATCTGCTACTTTATATTGCTATTGGTTGCAATCTCAAAATCCTAGTCAGTTGATAGGCAGAAATTCGTTTTTTTTTTTAAGTTGATTTAACATAGGGCTTGACCAATTACTATCTCACAGTTTCTGTAAGTCATTAGGACCAATTGTTCAATCATCTATCGTTTAGAGGTATGAAAGGCAAATGTTGAGATTTTCTTTAAAAATATTGTTTTGAATCTCAATTATTTTAGGATTGATCTCTACATGTATTGAGGTTTAATCTTGTAATTCTAATACACTAATCATTGGTGCGGATTTTCCTGCCATGTTATTCGTTTTCTATACTGACCATCTAGATTCATTTGTTTTCCTTTTATGTAATTATGTCAGGTTGTAATTTACTCTCCTGCTAGAACTGCATCCCAACAAGGATCAGGCAAGGTTGGCAAGTGGAAAATCAATTTTGTGTCAACACAAAAGTATGAATTCTTATCTGATTGTTTTCTTTATTTTTTTCTGAGTTTGATCTTAAAGGGAAAAGAAATCAGAAAATTGTTGCGTCGATATTTAACAGCAATTTTAGTTCAGGTCTAGCTGATTTCTAGATGCTAGATTGAAATCTTGTGCAGAACATGTTATACTGGGCAAACTTCAAGTGCCCAAAAGTTACTGGTCTTTGTTCTTTTACTCTTTACAATTACAGTTTTTTTACCCTTATAATTTTTTTCTTTTTGAAATCTTGTTTCTCTGATCAAGCACCTTTTCTGATGGATTGGTTCCATTTTCTATTGCCTGTGTAATATTTAATTTGTTTTCATAAATGTGTGGTTTGTCGTTTAGAGAAGGAAAATATCATACCGAAGGGCAAAAGTTTTTTATGCTTATCTGTCATGTTTGTAGGTGGGAGAACCCATTGATGGGGTGGACTTCTACTGGGGATCCCTATGCCAATGTAGGGGATTCTGCACTGTCTTTTGATAGCGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGATGGGAATATGTGGTAATGCTTGCTTTAACCATTTAGAAAGATTTTTCTTCTTGTAATACCTTTATGTAGTTTGAGTTGTCATATCCTAGGTTAGTTATTAATTATGTTGTTTTTCCACTGTCAGTTTCCTTCAAATAAGTAATACTTAGAGTCGGTAGTGTTGATTAATTGTGCACATACTCTAATATATTCTTGACAACAATCTTCGCATTTTCACAAGGTTGAAAGTGGAACAATGCATTCTGATTCTAAGTAAACTCGAAAGAGCCAAAAAGACAGCCTTAAGGGCAGGGGTTGAGGAAATCCCCTCCCATAAAACTAATATGGAACAAATAAATAATACTGAAATGAAATTGACATTGGATATGGTTGTTTAGTATGTTTATTTAGAATAACAAGTATGATTGATGATTTCTGCTTTTGGGCTATAACATGGTGTTTGACTCTAAATGTAAGATCTTATTTTCGAAGATAGTGGAAATTTAGTCAGTTATATAATATCCCAGTATTAGTTTGAAGCTAGTCTTCATAAGCTTCGAGAGGAGAAATATGAGGGGGAGAAGTCAGTTTTTCTTTCATTAGATTAAGAAAATTTATATTTTTTATTTTAGCATTCTCTTAACATTGCATTACCATTCTGTTTTTGTCCGGAGTGATTAAAAGTTTCTTTAATGTTCAGATTGAGGAAATACTTTTCTGGCCTACATATAATTATGAGACTACATTCATACCATCTCCAAAATTTGGGTTAACTTCTAATCTTTCTGTTGATTGTGTTTCTTGATTTTCTGTTTTTTTCCCCTTCTTTTTGTAGGTTAAGAAGCGCCACACACCACTTTTGAAGGTTTGAGATTCTTTCCCATTATTTCAATTTCACTGAGCTTGTTAACTGTCTTTTTTAGTTCCGTGGGGGTTGGATGCCCAAGTATCGTCCATTGAGCTTTCTGTCTTATTTACAAGCATCGATTATTTCCAAGGAATAAGCCCTTTTGTTAAGTTTATACATTTGATCAATGCAACTTCTTTCATCATCTTCTAGAATTCTGCTGGCGTCTTTTGGTAATTATAAATCCTTTTGCAATATAGTCTTGTGAATAGTTTAGGGGAGATCAGTTTCAGTTTCCTCTTGGTACCTGTAGTTATTTCTGCTTTCTAGATGACTTAAAATTTTATTTGTTATTATTAGAGTACTTTATAGTCCTACTCCTAACTAACAGGTCAGAGTTCCGTTTAAGATAGATGAAGGAGAAAGATGAAGTTCGGACAAATTGGTTTGAAAGTCAATCATGTTTAACACCCACTGATGAAGGAATTTTGATGCTGCTTGTAATTTTTATATCATCTAACTAATGGGAGCATCCAAAATAAAGAAACAGAAAAAACTTACTACTCCCTGCTTTGACTGTAGGCGAAGGCATACGCAGACAATTTCAAATGGAAGGGCCCTCCTGCTGCAGCCAAGGAGGCTTAGACCTTGTTTGACACTCCTTTTCTTATTTAATTGGAGGCTGAACAGACTGGAAGGTTTGTGTAGTCCGTTTCGTTGCTTATTTTTGAGGAAGTTGGTTGTAATAAGTGAGAACCGTGGTGTATGCTCACTTGACCTTGTAACTTTGTTCTCTCTTTACGTTACCGTTACAGAACACCAAATGAAAAGTCTCAAGAAACAAACGAGCATAGGAATTGATAAGTTGATTGTTTTGCGCGATGAATGAAATTTTTTAGCAATAGTATAGCGCAATTCCATCAACATTGTTTAGATCAGTGCCTATTAACTTTTAGTTATATCAGTCCACAAAGTATGGTCCGTGTGCAAAATGTTATCAAGAGCGCAGCTGTAACTTTGTAGTACTGACAATTTTCTCCTCCAGACAACACTTGACTCCCTTTCTAATTTTCTTCTTCACGAGACAGCAG

mRNA sequence

GTCAGCCAAATGAGTTACGTTCGGCCTCTCTCTTATTTGATGTTTTTCGTATTATTTTAAAGGCCAACCTCCATTTTCGACAAGTTTTAAACAAAACAACACCTTAAAATAAATCTCTTCTCCGTCGTTCAAAGTTCAATCGAGTTGGAAGTTTGTTTGTAACGGGGGACGAGGCTGACGGAAAACTAGTTTTCGCGTCGAATTTTCTTCATCCACGGATGGCTAACTCCTTGCAGCGAATCTGGAGCCACGGACGTGCTCTTCGGGGTACTCTGGCGCCTCTTTCCAGGTCATTCTCCACCGATTCCTTGGTTGAAGTGAAGCCTGGCGAGATCGGTATAGTCTCTGGAATTCCAGAGGAGCATCTTACTAGAAGGGTTGTAATTTACTCTCCTGCTAGAACTGCATCCCAACAAGGATCAGGCAAGGTTGGCAAGTGGAAAATCAATTTTGTGTCAACACAAAAGTGGGAGAACCCATTGATGGGGTGGACTTCTACTGGGGATCCCTATGCCAATGTAGGGGATTCTGCACTGTCTTTTGATAGCGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGATGGGAATATGTGGTTAAGAAGCGCCACACACCACTTTTGAAGGCGAAGGCATACGCAGACAATTTCAAATGGAAGGGCCCTCCTGCTGCAGCCAAGGAGGCTTAGACCTTGTTTGACACTCCTTTTCTTATTTAATTGGAGGCTGAACAGACTGGAAGGTTTGTGTAGTCCGTTTCGTTGCTTATTTTTGAGGAAGTTGGTTGTAATAAGTGAGAACCGTGGTGTATGCTCACTTGACCTTGTAACTTTGTTCTCTCTTTACGTTACCGTTACAGAACACCAAATGAAAAGTCTCAAGAAACAAACGAGCATAGGAATTGATAAGTTGATTGTTTTGCGCGATGAATGAAATTTTTTAGCAATAGTATAGCGCAATTCCATCAACATTGTTTAGATCAGTGCCTATTAACTTTTAGTTATATCAGTCCACAAAGTATGGTCCGTGTGCAAAATGTTATCAAGAGCGCAGCTGTAACTTTGTAGTACTGACAATTTTCTCCTCCAGACAACACTTGACTCCCTTTCTAATTTTCTTCTTCACGAGACAGCAG

Coding sequence (CDS)

ATGGCTAACTCCTTGCAGCGAATCTGGAGCCACGGACGTGCTCTTCGGGGTACTCTGGCGCCTCTTTCCAGGTCATTCTCCACCGATTCCTTGGTTGAAGTGAAGCCTGGCGAGATCGGTATAGTCTCTGGAATTCCAGAGGAGCATCTTACTAGAAGGGTTGTAATTTACTCTCCTGCTAGAACTGCATCCCAACAAGGATCAGGCAAGGTTGGCAAGTGGAAAATCAATTTTGTGTCAACACAAAAGTGGGAGAACCCATTGATGGGGTGGACTTCTACTGGGGATCCCTATGCCAATGTAGGGGATTCTGCACTGTCTTTTGATAGCGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGATGGGAATATGTGGTTAAGAAGCGCCACACACCACTTTTGAAGGCGAAGGCATACGCAGACAATTTCAAATGGAAGGGCCCTCCTGCTGCAGCCAAGGAGGCTTAG

Protein sequence

MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPARTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA*
Homology
[Close]
BLAST of CSPI06G28970 vs. ExPASy Swiss-Prot
Match: Q9FJW4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 6.8e-57
Identity = 104/138 (75.36%), Postives = 122/138 (88.41%), Query Frame = 0 

Query: 15  LRGTLAPLSRSFSTDSLVE--VKPGEIGIVSGIPEEHLTRRVVIYSPARTASQQGSGKVG 74
           +  TL  ++R F+TD++VE   K GEIG VSGIPEEHL+R+V+IYSPARTA+Q GSGK+G
Sbjct: 13  IAATLRRVARPFATDAVVESDYKRGEIGKVSGIPEEHLSRKVIIYSPARTATQSGSGKLG 72

Query: 75  KWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHT 134
           KWKINFVST KWENPLMGWTSTGDPYANVGDSAL+FDSEEAAK+FAE+HGW+Y VKK +T
Sbjct: 73  KWKINFVSTLKWENPLMGWTSTGDPYANVGDSALAFDSEEAAKSFAERHGWDYKVKKPNT 132

Query: 135 PLLKAKAYADNFKWKGPP 151
           PLLK K+Y+DNFKWKG P
Sbjct: 133 PLLKVKSYSDNFKWKGNP 150

[Close]
BLAST of CSPI06G28970 vs. ExPASy Swiss-Prot
Match: Q5XIF3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Rattus norvegicus OX=10116 GN=Ndufs4 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 6.9e-25
Identity = 56/121 (46.28%), Postives = 77/121 (63.64%), Query Frame = 0 

Query: 27  STDSLVEVKPGEIGIVSGIPEEHL-TRRVVIYSPARTASQQGSGKVGKWKINFVSTQKWE 86
           + D  ++V P     ++G+PEEH+ TR+V I+ PAR   Q G     KWK+ F + ++WE
Sbjct: 54  TVDEKLDVTP-----LTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWE 113

Query: 87  NPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKAKAYADNFK 146
           NPLMGW ST DP +N+    L+F ++E A AFAEKHGW Y V+ R  P  K+K+Y  NF 
Sbjct: 114 NPLMGWASTADPLSNM---VLTFSAKEDAVAFAEKHGWSYDVEGRKVPKPKSKSYGANFS 166

[Close]
BLAST of CSPI06G28970 vs. ExPASy Swiss-Prot
Match: Q02375 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Bos taurus OX=9913 GN=NDUFS4 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 3.4e-24
Identity = 52/110 (47.27%), Postives = 73/110 (66.36%), Query Frame = 0 

Query: 38  EIGIVSGIPEEHL-TRRVVIYSPARTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGD 97
           +I  ++G+PEEH+ TR+  I+ PAR   Q G     KWK+ F + ++WENPLMGW ST D
Sbjct: 60  DITTITGVPEEHIKTRKARIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTAD 119

Query: 98  PYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKAKAYADNFKW 147
           P +N+    L+F ++E A AFAEK+GW Y V++R  P  K+K+Y  NF W
Sbjct: 120 PLSNL---VLTFSTKEDAVAFAEKNGWSYDVEERKVPKPKSKSYGANFSW 166

[Close]
BLAST of CSPI06G28970 vs. ExPASy Swiss-Prot
Match: Q66XS7 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gekko japonicus OX=146911 GN=NDUFS4 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 4.5e-24
Identity = 52/110 (47.27%), Postives = 75/110 (68.18%), Query Frame = 0 

Query: 38  EIGIVSGIPEEHL-TRRVVIYSPARTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGD 97
           +I  ++G+PEEH+ TR+V I+ PAR   Q G     KWK+ F + ++WENPLMGW+ST D
Sbjct: 60  DITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWSSTAD 119

Query: 98  PYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKAKAYADNFKW 147
           P +N+    L+F ++E A AFAEK+GW + V++R  P  K+K+Y  NF W
Sbjct: 120 PLSNL---VLTFSTKEDAVAFAEKNGWSFDVEERKVPKPKSKSYGANFSW 166

[Close]
BLAST of CSPI06G28970 vs. ExPASy Swiss-Prot
Match: Q0MQH0 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gorilla gorilla gorilla OX=9595 GN=NDUFS4 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 5.8e-24
Identity = 51/110 (46.36%), Postives = 74/110 (67.27%), Query Frame = 0 

Query: 38  EIGIVSGIPEEHL-TRRVVIYSPARTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGD 97
           +I  ++G+PEEH+ TR+V I+ PAR   Q G     KWK+ F + ++WENPLMGW ST D
Sbjct: 60  DITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTAD 119

Query: 98  PYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKAKAYADNFKW 147
           P +N+    L+F ++E A +FAEK+GW Y +++R  P  K+K+Y  NF W
Sbjct: 120 PLSNM---VLTFSTKEDAVSFAEKNGWSYDIEERKVPKPKSKSYGANFSW 166

[Close]
BLAST of CSPI06G28970 vs. ExPASy TrEMBL
Match: A0A0A0KGI3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis sativus OX=3659 GN=Csa_6G495720 PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 5.2e-84
Identity = 154/156 (98.72%), Postives = 156/156 (100.00%), Query Frame = 0 

Query: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVE+KPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEMKPGEIGVVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 157
           HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 156

[Close]
BLAST of CSPI06G28970 vs. ExPASy TrEMBL
Match: A0A5D3CP24 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003960 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 3.7e-82
Identity = 151/156 (96.79%), Postives = 154/156 (98.72%), Query Frame = 0 

Query: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWSHGRALR T+APLSRSFSTDSLV+VKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSHGRALRSTVAPLSRSFSTDSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 157
           HGWEYVVKKRHTPLLKAKAYADNFKWKGPPA AKEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAEAKEA 156

[Close]
BLAST of CSPI06G28970 vs. ExPASy TrEMBL
Match: A0A1S3B1N9 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103484861 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 3.7e-82
Identity = 151/156 (96.79%), Postives = 154/156 (98.72%), Query Frame = 0 

Query: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWSHGRALR T+APLSRSFSTDSLV+VKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSHGRALRSTVAPLSRSFSTDSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 157
           HGWEYVVKKRHTPLLKAKAYADNFKWKGPPA AKEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAEAKEA 156

[Close]
BLAST of CSPI06G28970 vs. ExPASy TrEMBL
Match: A0A6J1BSY3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111005430 PE=3 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.3e-76
Identity = 143/156 (91.67%), Postives = 148/156 (94.87%), Query Frame = 0 

Query: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW+H   LRGTLAPL RSFS DSLV+VKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINF+STQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 157
           HGW+YVVKKRHTPLLK KAYADNFKWKGPP  AKEA
Sbjct: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPP-EAKEA 155

[Close]
BLAST of CSPI06G28970 vs. ExPASy TrEMBL
Match: A0A6J1HE01 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111463327 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.1e-76
Identity = 141/150 (94.00%), Postives = 145/150 (96.67%), Query Frame = 0 

Query: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW+ G ALRGT+APL RSFS DSLVEVKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNRGCALRGTMAPLCRSFSADSLVEVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINF+STQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPP 151
           HGWEYVVKKRHTPLLKAKAYADNFKWKG P
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGHP 150

[Close]
BLAST of CSPI06G28970 vs. NCBI nr
Match: XP_004141860.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucumis sativus] >KGN48618.1 hypothetical protein Csa_004287 [Cucumis sativus])

HSP 1 Score: 320.1 bits (819), Expect = 1.1e-83
Identity = 154/156 (98.72%), Postives = 156/156 (100.00%), Query Frame = 0 

Query: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVE+KPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEMKPGEIGVVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 157
           HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 156

[Close]
BLAST of CSPI06G28970 vs. NCBI nr
Match: XP_008440406.1 (PREDICTED: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucumis melo] >KAA0036408.1 NADH dehydrogenase (ubiquinone) iron-sulfur protein 4 [Cucumis melo var. makuwa] >TYK12804.1 NADH dehydrogenase (ubiquinone) iron-sulfur protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 313.9 bits (803), Expect = 7.7e-82
Identity = 151/156 (96.79%), Postives = 154/156 (98.72%), Query Frame = 0 

Query: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWSHGRALR T+APLSRSFSTDSLV+VKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSHGRALRSTVAPLSRSFSTDSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 157
           HGWEYVVKKRHTPLLKAKAYADNFKWKGPPA AKEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAEAKEA 156

[Close]
BLAST of CSPI06G28970 vs. NCBI nr
Match: XP_038881462.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 300.4 bits (768), Expect = 8.8e-78
Identity = 145/156 (92.95%), Postives = 151/156 (96.79%), Query Frame = 0 

Query: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWSHG ALRGT+APL RSFSTDSLV+VKPGEIG+VSGIPEEHLTRRV+IYSPA
Sbjct: 1   MANSLQRIWSHGCALRGTVAPLCRSFSTDSLVQVKPGEIGMVSGIPEEHLTRRVIIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINF+STQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 157
           HGWEYVV+KRHTPLLK KAYADNFKWKGPP  AKEA
Sbjct: 121 HGWEYVVQKRHTPLLKVKAYADNFKWKGPP-EAKEA 155

[Close]
BLAST of CSPI06G28970 vs. NCBI nr
Match: XP_022132610.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Momordica charantia])

HSP 1 Score: 294.7 bits (753), Expect = 4.8e-76
Identity = 143/156 (91.67%), Postives = 148/156 (94.87%), Query Frame = 0 

Query: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW+H   LRGTLAPL RSFS DSLV+VKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINF+STQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 157
           HGW+YVVKKRHTPLLK KAYADNFKWKGPP  AKEA
Sbjct: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPP-EAKEA 155

[Close]
BLAST of CSPI06G28970 vs. NCBI nr
Match: XP_022962977.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita moschata] >XP_022962978.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita moschata] >XP_023003205.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita maxima] >XP_023517797.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita pepo subsp. pepo] >KAG6594972.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 294.3 bits (752), Expect = 6.3e-76
Identity = 141/150 (94.00%), Postives = 145/150 (96.67%), Query Frame = 0 

Query: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW+ G ALRGT+APL RSFS DSLVEVKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNRGCALRGTMAPLCRSFSADSLVEVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINF+STQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPP 151
           HGWEYVVKKRHTPLLKAKAYADNFKWKG P
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGHP 150

[Close]
BLAST of CSPI06G28970 vs. TAIR 10
Match: AT5G67590.1 (NADH-ubiquinone oxidoreductase-related )

HSP 1 Score: 221.5 bits (563), Expect = 4.9e-58
Identity = 104/138 (75.36%), Postives = 122/138 (88.41%), Query Frame = 0 

Query: 15  LRGTLAPLSRSFSTDSLVE--VKPGEIGIVSGIPEEHLTRRVVIYSPARTASQQGSGKVG 74
           +  TL  ++R F+TD++VE   K GEIG VSGIPEEHL+R+V+IYSPARTA+Q GSGK+G
Sbjct: 13  IAATLRRVARPFATDAVVESDYKRGEIGKVSGIPEEHLSRKVIIYSPARTATQSGSGKLG 72

Query: 75  KWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHT 134
           KWKINFVST KWENPLMGWTSTGDPYANVGDSAL+FDSEEAAK+FAE+HGW+Y VKK +T
Sbjct: 73  KWKINFVSTLKWENPLMGWTSTGDPYANVGDSALAFDSEEAAKSFAERHGWDYKVKKPNT 132

Query: 135 PLLKAKAYADNFKWKGPP 151
           PLLK K+Y+DNFKWKG P
Sbjct: 133 PLLKVKSYSDNFKWKGNP 150

The following BLAST results are available for this feature:
BLAST of CSPI06G28970 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FJW46.8e-5775.36NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Arabidop... [more]
Q5XIF36.9e-2546.28NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Rattus n... [more]
Q023753.4e-2447.27NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Bos taur... [more]
Q66XS74.5e-2447.27NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gekko ja... [more]
Q0MQH05.8e-2446.36NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gorilla ... [more]
BLAST of CSPI06G28970 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KGI35.2e-8498.72NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis ... [more]
A0A5D3CP243.7e-8296.79NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis ... [more]
A0A1S3B1N93.7e-8296.79NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis ... [more]
A0A6J1BSY32.3e-7691.67NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Momordic... [more]
A0A6J1HE013.1e-7694.00NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbit... [more]
BLAST of CSPI06G28970 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004141860.11.1e-8398.72NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucumis sa... [more]
XP_008440406.17.7e-8296.79PREDICTED: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial ... [more]
XP_038881462.18.8e-7892.95NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Benincasa ... [more]
XP_022132610.14.8e-7691.67NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Momordica ... [more]
XP_022962977.16.3e-7694.00NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucur... [more]
BLAST of CSPI06G28970 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G67590.14.9e-5875.36NADH-ubiquinone oxidoreductase-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006885NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrialPFAMPF04800ETC_C1_NDUFA4coord: 53..147
e-value: 5.9E-33
score: 113.0
IPR006885NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrialPANTHERPTHR12219NADH-UBIQUINONE OXIDOREDUCTASEcoord: 13..152
IPR038532NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamilyGENE3D3.30.160.190atu1810 like domaincoord: 52..127
e-value: 6.5E-34
score: 117.7
NoneNo IPR availablePANTHERPTHR12219:SF23NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL-LIKEcoord: 13..152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G28970.1CSPI06G28970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022900 electron transport chain
cellular_component GO:0005743 mitochondrial inner membrane
cellular_component GO:0070469 respirasome
molecular_function GO:0016651 oxidoreductase activity, acting on NAD(P)H