CSPI06G27750 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G27750
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	AB hydrolase-1 domain-containing protein
Location	Chr6: 24337768 .. 24339524 (-)
RNA-Seq Expression	CSPI06G27750
Synteny	CSPI06G27750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCAAATGAAGCATTTTGTTCTGGTTCATGGAGCTTGTCATGGAGCATGGTGTTGGTACAAGATCAAGCTGTTGCTAGAGGCGGCTGGTCACCGTGTAACAATGTTAGACATGGCGGGGGCCGGGGTAAACAGGAAGGCAATCCAGGAGGTTGAGTCATTTGAAGAGTATTCAGAACCATTGTTGAAGACCATGGCTTGTTTGGGTCCAAACGAAAAGGTTATATTGGTTGGCCATAGCTTTGGTGGCATGAGTTTGGCTTTGGCCATGGAGAATTTCCCTCACAAAATATCAGCTTCTGTTTTCGTAACTGCCTTTGTTCCTGATACCCATCACCCACCTTCCTATGTCCTAGAACAGGTAAGAGATCTTTTAGTTCAATCTTCCCATTCTTAATATACTTGAAAAAAGTGATTATTATTCGATGAAGTATTAAAGACCAATCCATTGATTGTTTCAATCAAAAGTCCGTTTAGTATATGCAACCCAAGTCTAAGATATATGTATGGTATATAAGTGATAACATAGTTACGTTTTTTATTATTGTTGAATGAAGTTTCTTGAGAGCCTTCCCAGAGAATTTTGGATGGACACAGAGTTTGGTGAAAACAGAGAGGATGGAGGTTCTTCAAGTTGGTTTTTATTTGGGCCCAAATGCATGGCCAATAAAATCTATCAACTCTCCCATACTGAGGTATCCTGATCTATATATTTCTCCTTTTTCTATTAATAATTACAAAGGAGTGTTGATTTATTATTGTTTGATTTTATGATTATGAAAAGGATCAAGCTTTGGGGAGTAGTCTGGTGAGACCAGCCAAACTATTCATATAAAACTTGGGAAAAGCAGAGAAATTTACAGAGGAAAATTATGGGTCAGTGAAGAAAGTGTATGTGATTGGTGGTGAAGACAGAACAATCCCAAAGCAGTTACAAAAATGGATGATTCAGAATAGTGATAAGAGGATTCAAAATGTAATGGAAATTGATGAAGCTGATCACATAGAAAAAATTAAAGACTAGAAATAGAAATCCCCGTAAATGTGGCAGGGTCAAATCACAGGGATGAAAATCACCCAAATACCTTACAGATGAATTACAAAATGGCATTTCAATGGAAACATGACACCAAATGAAAATATAAATAGAGTGAAAAAGGGTCGAAAACTCACTACTAGTAATTTCAGTACACAAATTACACAAGTAATGCATATGTGAGTAATTGAAGTAAATAAAGTTAAAACATGCTGTAGAGATACGTTGATATATAAACCAACTAAGAGGTATAACTTGTCAATCCTGACGGCCGCTGTGAAGCAGCCACAGCCAGAGGTCGGTCCAAGAAGAATATTCGATAGATACTCCATCTTTGGAATGTAAGAGAATTCCACTTGGGCATATTCTTGTTTCCTGAAACCGCAAGTCAAGTATTCTGTACAGCAGGCCACTAAAAATGCCAAGAATGGCGTGAGATTAGGAAGATATATTGCGTTAGCATCCCTCGAATCAATTGCATCACAAGAAAAAACAAGTCTTGTTCTTTGAGTTTTCATAAGAATTGATCGCATCTATGCTGTATTCTACAGAGTTACAGAAAATTCTCAGGTCGCCTTCCGATCTCTTTCAGAAAGCAACGAGCAATGGTGGACGTTTTGAGAAAAGCCTTCCAATTTTCTTACAAGAAAGATTCCGATACCTCTAGGCTTTTCACTGCCAGTAAATTATCTGATGGCCTCTCCTCGAAGATGTACTGA

mRNA sequence

ATGGATCAAATGAAGCATTTTGTTCTGGTTCATGGAGCTTGTCATGGAGCATGGTGTTGGTACAAGATCAAGCTGTTGCTAGAGGCGGCTGGTCACCGTGTAACAATGTTAGACATGGCGGGGGCCGGGGTAAACAGGAAGGCAATCCAGGAGGTTGAGTCATTTGAAGAGTATTCAGAACCATTGTTGAAGACCATGGCTTGTTTGGGTCCAAACGAAAAGGTTATATTGGTTGGCCATAGCTTTGGTGGCATGAGTTTGGCTTTGGCCATGGAGAATTTCCCTCACAAAATATCAGCTTCTGTTTTCGTAACTGCCTTTGTTCCTGATACCCATCACCCACCTTCCTATGTCCTAGAACAGTTTCTTGAGAGCCTTCCCAGAGAATTTTGGATGGACACAGAGTTTGGTGAAAACAGAGAGGATGGAGGTTCTTCAAGTTGGTTTTTATTTGGGCCCAAATGCATGGCCAATAAAATCTATCAACTCTCCCATACTGAGTCTGGTGAGACCAGCCAAACTATTCATATAAAACTTGGGAAAAGCAGAGAAATTTACAGAGGAAAATTATGGGTCAGTGAAGAAAGTAATTCCACTTGGGCATATTCTTGTTTCCTGAAACCGCAAGTCAAGTATTCTGTACAGCAGGCCACTAAAAATGCCAAGAATGGCAGTTACAGAAAATTCTCAGGTCGCCTTCCGATCTCTTTCAGAAAGCAACGAGCAATGGTGGACGTTTTGAGAAAAGCCTTCCAATTTTCTTACAAGAAAGATTCCGATACCTCTAGGCTTTTCACTGCCAGTAAATTATCTGATGGCCTCTCCTCGAAGATGTACTGA

Coding sequence (CDS)

ATGGATCAAATGAAGCATTTTGTTCTGGTTCATGGAGCTTGTCATGGAGCATGGTGTTGGTACAAGATCAAGCTGTTGCTAGAGGCGGCTGGTCACCGTGTAACAATGTTAGACATGGCGGGGGCCGGGGTAAACAGGAAGGCAATCCAGGAGGTTGAGTCATTTGAAGAGTATTCAGAACCATTGTTGAAGACCATGGCTTGTTTGGGTCCAAACGAAAAGGTTATATTGGTTGGCCATAGCTTTGGTGGCATGAGTTTGGCTTTGGCCATGGAGAATTTCCCTCACAAAATATCAGCTTCTGTTTTCGTAACTGCCTTTGTTCCTGATACCCATCACCCACCTTCCTATGTCCTAGAACAGTTTCTTGAGAGCCTTCCCAGAGAATTTTGGATGGACACAGAGTTTGGTGAAAACAGAGAGGATGGAGGTTCTTCAAGTTGGTTTTTATTTGGGCCCAAATGCATGGCCAATAAAATCTATCAACTCTCCCATACTGAGTCTGGTGAGACCAGCCAAACTATTCATATAAAACTTGGGAAAAGCAGAGAAATTTACAGAGGAAAATTATGGGTCAGTGAAGAAAGTAATTCCACTTGGGCATATTCTTGTTTCCTGAAACCGCAAGTCAAGTATTCTGTACAGCAGGCCACTAAAAATGCCAAGAATGGCAGTTACAGAAAATTCTCAGGTCGCCTTCCGATCTCTTTCAGAAAGCAACGAGCAATGGTGGACGTTTTGAGAAAAGCCTTCCAATTTTCTTACAAGAAAGATTCCGATACCTCTAGGCTTTTCACTGCCAGTAAATTATCTGATGGCCTCTCCTCGAAGATGTACTGA

Protein sequence

MDQMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSEPLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQFLESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGETSQTIHIKLGKSREIYRGKLWVSEESNSTWAYSCFLKPQVKYSVQQATKNAKNGSYRKFSGRLPISFRKQRAMVDVLRKAFQFSYKKDSDTSRLFTASKLSDGLSSKMY*

Homology

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BLAST of CSPI06G27750 vs. ExPASy Swiss-Prot
Match: Q8S8S9 (Methylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=MES1 PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 2.1e-48
Identity = 92/167 (55.09%), Postives = 122/167 (73.05%), Query Frame = 0


Query: 5   KHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVN-RKAIQEVESFEEYSEPLL 64
           +HFVLVHG+CHGAWCWYK+K LLEA GHRVT +D+A +G++  ++I ++ + E+YSEPL 
Sbjct: 8   QHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPLT 67

Query: 65  KTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQFL 124
           K +  L  +EKV+LVGHSFGG++LA+AME FP KIS +VF+TAF+PDT H PS+VL++F 
Sbjct: 68  KLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKFG 127

Query: 125 ESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGE 171
            ++P+E WM TEF     D    S F F P  M   +YQLS  E  E
Sbjct: 128 SNMPQEAWMGTEFEPYGSDNSGLSMF-FSPDFMKLGLYQLSPVEDLE 173

[Close]

BLAST of CSPI06G27750 vs. ExPASy Swiss-Prot
Match: O80477 (Methylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=MES3 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.0e-47
Identity = 89/175 (50.86%), Postives = 126/175 (72.00%), Query Frame = 0


Query: 2   DQMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVN-RKAIQEVESFEEYSE 61
           ++ +H VLVHGACHGAWCWYK+K  LEA+GHRVT +D+A +G++  ++I ++ + E+YSE
Sbjct: 5   ERKQHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSE 64

Query: 62  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLE 121
           PL++ M  L  +EKV+LVGHS GG+SLA+AM+ FP KIS SVFVTA +PDT H PS+V +
Sbjct: 65  PLMQLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVWD 124

Query: 122 QFLESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGETSQTI 176
           +  +   RE W+DT F   + D  S  W +FGP+ MA  +YQLS  +  E ++ +
Sbjct: 125 KLRKETSREEWLDTVFTSEKPDFPSEFW-IFGPEFMAKNLYQLSPVQDLELAKML 178

[Close]

BLAST of CSPI06G27750 vs. ExPASy Swiss-Prot
Match: O80476 (Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.8e-47
Identity = 94/167 (56.29%), Postives = 120/167 (71.86%), Query Frame = 0


Query: 5   KHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVN-RKAIQEVESFEEYSEPLL 64
           +HFVLVHGACHGAWCWYK+K LLEA GHRVT LD+A +G++  ++I ++ + E+YSEPL+
Sbjct: 8   QHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPLM 67

Query: 65  KTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQFL 124
           + M  L  +EKV+LVGHSFGG+SLALAM+ FP KIS SVFVTAF+PDT H PS+V E+F 
Sbjct: 68  QLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKFA 127

Query: 125 ESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGE 171
            S+  E WM +E      D    S F F    M +++YQLS  E  E
Sbjct: 128 SSMTPEGWMGSELETYGSDNSGLSVF-FSTDFMKHRLYQLSPVEDLE 173

[Close]

BLAST of CSPI06G27750 vs. ExPASy Swiss-Prot
Match: Q6RYA0 (Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 5.7e-46
Identity = 88/167 (52.69%), Postives = 117/167 (70.06%), Query Frame = 0


Query: 1   MDQMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSE 60
           M + KHFVLVHGACHG W WYK+K LLEAAGH+VT LD+A +G + + I+E+ +  +Y+ 
Sbjct: 1   MKEGKHFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTL 60

Query: 61  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLE 120
           PL++ M  L  +EKVILVGHS GGM+L LAME +P KI A+VF+ AF+PD+ H  S+VLE
Sbjct: 61  PLMELMESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFVLE 120

Query: 121 QFLESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTE 168
           Q+ E  P E W+DT+F          +   FGPK +A+K+YQL   E
Sbjct: 121 QYNERTPAENWLDTQFLPYGSPEEPLTSMFFGPKFLAHKLYQLCSPE 167

[Close]

BLAST of CSPI06G27750 vs. ExPASy Swiss-Prot
Match: Q9SE93 (Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.8e-44
Identity = 84/175 (48.00%), Postives = 123/175 (70.29%), Query Frame = 0


Query: 3   QMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSEPL 62
           Q KHFVLVHG C GAW WYK+K LLE+AGH+VT +D++ AG+N + + E+ +F +YSEPL
Sbjct: 9   QQKHFVLVHGGCLGAWIWYKLKPLLESAGHKVTAVDLSAAGINPRRLDEIHTFRDYSEPL 68

Query: 63  LKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQF 122
           ++ MA + P+EKV+L+GHSFGGMSL LAME +P KIS +VF++A +PD +H  +Y  E++
Sbjct: 69  MEVMASIPPDEKVVLLGHSFGGMSLGLAMETYPEKISVAVFMSAMMPDPNHSLTYPFEKY 128

Query: 123 LESLPREFWMDTEFGE--NREDGGSSSWFLFGPKCMANKIYQLSHTESGETSQTI 176
            E  P +  +D++F    N E+ G S   + GP+ MA K++Q    E  E ++ +
Sbjct: 129 NEKCPADMMLDSQFSTYGNPENPGMS--MILGPQFMALKMFQNCSVEDLELAKML 181

[Close]

BLAST of CSPI06G27750 vs. ExPASy TrEMBL
Match: A0A0A0KG72 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490080 PE=4 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 7.8e-91
Identity = 161/167 (96.41%), Postives = 163/167 (97.60%), Query Frame = 0


Query: 1   MDQMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSE 60
           MDQMKHFVLVHGACHGAWCWYKIK LLEAAGHRVTMLDM GAGVNR+AIQEVESFEEYSE
Sbjct: 1   MDQMKHFVLVHGACHGAWCWYKIKPLLEAAGHRVTMLDMGGAGVNRRAIQEVESFEEYSE 60

Query: 61  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLE 120
           PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVF+TAFVPDTHHPPSYVLE
Sbjct: 61  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFITAFVPDTHHPPSYVLE 120

Query: 121 QFLESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTE 168
           QFLESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQ S TE
Sbjct: 121 QFLESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQFSPTE 167

[Close]

BLAST of CSPI06G27750 vs. ExPASy TrEMBL
Match: A0A0A0KFL1 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490050 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 5.1e-90
Identity = 161/167 (96.41%), Postives = 163/167 (97.60%), Query Frame = 0


Query: 1   MDQMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSE 60
           MDQMKHFVLVHGACHGAWCWYKIK LLEAAGHRVTMLDMAGAGVNR+AIQEV+SFEEYSE
Sbjct: 1   MDQMKHFVLVHGACHGAWCWYKIKPLLEAAGHRVTMLDMAGAGVNRRAIQEVKSFEEYSE 60

Query: 61  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLE 120
           PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLE
Sbjct: 61  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLE 120

Query: 121 QFLESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTE 168
           QFLESLPREFW DTE GENREDGGSSSWFLFGPKCMANKIYQLS TE
Sbjct: 121 QFLESLPREFWRDTELGENREDGGSSSWFLFGPKCMANKIYQLSPTE 167

[Close]

BLAST of CSPI06G27750 vs. ExPASy TrEMBL
Match: A0A6J1IQY1 (salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477637 PE=4 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.9e-68
Identity = 128/173 (73.99%), Postives = 145/173 (83.82%), Query Frame = 0


Query: 3   QMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSEPL 62
           + KHFVLVHGACHGAW WYKIK LLEAAGHRVT+LDMAGAGVN KAIQEV+SF EYSEPL
Sbjct: 47  EQKHFVLVHGACHGAWTWYKIKPLLEAAGHRVTVLDMAGAGVNMKAIQEVKSFVEYSEPL 106

Query: 63  LKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQF 122
           L+ ++C+GPNEKVILVGHS GGMSLA+AME FPHKI+ASVFVTAFVPDTHH PS V+EQF
Sbjct: 107 LQALSCVGPNEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSDVIEQF 166

Query: 123 LESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGETSQTI 176
            ESLP+EFW DT+   N +    SSWF+FGPKC AN IYQLS +E    + T+
Sbjct: 167 RESLPKEFWQDTQVSTNDK---PSSWFIFGPKCTANNIYQLSPSEDLALAMTL 216

[Close]

BLAST of CSPI06G27750 vs. ExPASy TrEMBL
Match: A0A6J1GDS1 (salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111453076 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 2.7e-67
Identity = 127/173 (73.41%), Postives = 143/173 (82.66%), Query Frame = 0


Query: 3   QMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSEPL 62
           + KHFV+VHGACHGAW WYKIK LLEAAGHRV +LDMAGAGVN KAIQEV+SF EYSEPL
Sbjct: 24  EQKHFVMVHGACHGAWTWYKIKPLLEAAGHRVMVLDMAGAGVNMKAIQEVKSFVEYSEPL 83

Query: 63  LKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQF 122
           L+ M+C+GPNEKVILVGHS GGMSLA+AME FPHKI+ASVFVTAFVPDTHH PS V+EQF
Sbjct: 84  LQAMSCVGPNEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSDVIEQF 143

Query: 123 LESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGETSQTI 176
            ESLP EFW DT+   N +    SSWF+FGPKC AN IYQLS +E    + T+
Sbjct: 144 RESLPMEFWQDTQVPTNDK---PSSWFIFGPKCTANNIYQLSPSEDLALAMTL 193

[Close]

BLAST of CSPI06G27750 vs. ExPASy TrEMBL
Match: A0A5D3CMY2 (Methylesterase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005100 PE=4 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 1.1e-60
Identity = 114/121 (94.21%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 1   MDQMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSE 60
           MDQ KHFVLVHGACHGAWCWYKIK LLE AGHRVTMLDMAGAGVNRKAIQEV+SFEEYSE
Sbjct: 1   MDQKKHFVLVHGACHGAWCWYKIKPLLEVAGHRVTMLDMAGAGVNRKAIQEVKSFEEYSE 60

Query: 61  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLE 120
           PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVF+TAF+PDTHHPPSYV+E
Sbjct: 61  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFLTAFLPDTHHPPSYVVE 120

Query: 121 Q 122
           Q
Sbjct: 121 Q 121

[Close]

BLAST of CSPI06G27750 vs. NCBI nr
Match: XP_011657843.2 (salicylic acid-binding protein 2 [Cucumis sativus] >KAE8647492.1 hypothetical protein Csa_003158 [Cucumis sativus])

HSP 1 Score: 344.7 bits (883), Expect = 7.3e-91
Identity = 162/167 (97.01%), Postives = 163/167 (97.60%), Query Frame = 0


Query: 1   MDQMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSE 60
           MDQMKHFVLVHGACHGAWCWYKIK LLEAAGHRVTMLDM GAGVNRKAIQEVESFEEYSE
Sbjct: 1   MDQMKHFVLVHGACHGAWCWYKIKPLLEAAGHRVTMLDMGGAGVNRKAIQEVESFEEYSE 60

Query: 61  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLE 120
           PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVF+TAFVPDTHHPPSYVLE
Sbjct: 61  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFITAFVPDTHHPPSYVLE 120

Query: 121 QFLESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTE 168
           QFLESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQ S TE
Sbjct: 121 QFLESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQFSPTE 167

[Close]

BLAST of CSPI06G27750 vs. NCBI nr
Match: XP_004142018.1 (putative inactive methylesterase 20 [Cucumis sativus] >KGN48490.1 hypothetical protein Csa_003444 [Cucumis sativus])

HSP 1 Score: 340.9 bits (873), Expect = 1.1e-89
Identity = 161/167 (96.41%), Postives = 163/167 (97.60%), Query Frame = 0


Query: 1   MDQMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSE 60
           MDQMKHFVLVHGACHGAWCWYKIK LLEAAGHRVTMLDMAGAGVNR+AIQEV+SFEEYSE
Sbjct: 1   MDQMKHFVLVHGACHGAWCWYKIKPLLEAAGHRVTMLDMAGAGVNRRAIQEVKSFEEYSE 60

Query: 61  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLE 120
           PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLE
Sbjct: 61  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLE 120

Query: 121 QFLESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTE 168
           QFLESLPREFW DTE GENREDGGSSSWFLFGPKCMANKIYQLS TE
Sbjct: 121 QFLESLPREFWRDTELGENREDGGSSSWFLFGPKCMANKIYQLSPTE 167

[Close]

BLAST of CSPI06G27750 vs. NCBI nr
Match: XP_038881260.1 (salicylic acid-binding protein 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 280.8 bits (717), Expect = 1.3e-71
Identity = 144/195 (73.85%), Postives = 159/195 (81.54%), Query Frame = 0


Query: 3   QMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSEPL 62
           + +HFVLVHGACHGAWCWYKI+ LLEAAGHRVT+LDMAGAGV+RKAIQEV+S EEYSEPL
Sbjct: 2   EQRHFVLVHGACHGAWCWYKIQPLLEAAGHRVTVLDMAGAGVHRKAIQEVKSLEEYSEPL 61

Query: 63  LKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQF 122
           LKTMAC+GPNEKVILVGHSFGGMSLALAME FPH ISASVF+TA  PDTHH PSYV EQF
Sbjct: 62  LKTMACVGPNEKVILVGHSFGGMSLALAMEKFPHNISASVFLTALAPDTHHHPSYVSEQF 121

Query: 123 LESLPREFWMDTEF--GENR-EDGGSSSWFLFGPKCMANKIYQLSHTESGETSQTIHIKL 182
           LESLP+EFWMDT+    ENR +D  SSS+FLFGPKCMAN IY LS +E         + L
Sbjct: 122 LESLPKEFWMDTQSCGYENRVDDEASSSYFLFGPKCMANFIYHLSPSED--------LAL 181

Query: 183 GKSREIYRGKLWVSE 195
           GKS  +  G L V E
Sbjct: 182 GKSL-VRPGSLLVEE 187

[Close]

BLAST of CSPI06G27750 vs. NCBI nr
Match: XP_022977269.1 (salicylic acid-binding protein 2-like [Cucurbita maxima])

HSP 1 Score: 269.2 bits (687), Expect = 3.9e-68
Identity = 128/173 (73.99%), Postives = 145/173 (83.82%), Query Frame = 0


Query: 3   QMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSEPL 62
           + KHFVLVHGACHGAW WYKIK LLEAAGHRVT+LDMAGAGVN KAIQEV+SF EYSEPL
Sbjct: 47  EQKHFVLVHGACHGAWTWYKIKPLLEAAGHRVTVLDMAGAGVNMKAIQEVKSFVEYSEPL 106

Query: 63  LKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQF 122
           L+ ++C+GPNEKVILVGHS GGMSLA+AME FPHKI+ASVFVTAFVPDTHH PS V+EQF
Sbjct: 107 LQALSCVGPNEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSDVIEQF 166

Query: 123 LESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGETSQTI 176
            ESLP+EFW DT+   N +    SSWF+FGPKC AN IYQLS +E    + T+
Sbjct: 167 RESLPKEFWQDTQVSTNDK---PSSWFIFGPKCTANNIYQLSPSEDLALAMTL 216

[Close]

BLAST of CSPI06G27750 vs. NCBI nr
Match: XP_022949785.1 (salicylic acid-binding protein 2-like [Cucurbita moschata])

HSP 1 Score: 265.4 bits (677), Expect = 5.6e-67
Identity = 127/173 (73.41%), Postives = 143/173 (82.66%), Query Frame = 0


Query: 3   QMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSEPL 62
           + KHFV+VHGACHGAW WYKIK LLEAAGHRV +LDMAGAGVN KAIQEV+SF EYSEPL
Sbjct: 24  EQKHFVMVHGACHGAWTWYKIKPLLEAAGHRVMVLDMAGAGVNMKAIQEVKSFVEYSEPL 83

Query: 63  LKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQF 122
           L+ M+C+GPNEKVILVGHS GGMSLA+AME FPHKI+ASVFVTAFVPDTHH PS V+EQF
Sbjct: 84  LQAMSCVGPNEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSDVIEQF 143

Query: 123 LESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGETSQTI 176
            ESLP EFW DT+   N +    SSWF+FGPKC AN IYQLS +E    + T+
Sbjct: 144 RESLPMEFWQDTQVPTNDK---PSSWFIFGPKCTANNIYQLSPSEDLALAMTL 193

[Close]

BLAST of CSPI06G27750 vs. TAIR 10
Match: AT2G23620.1 (methyl esterase 1 )

HSP 1 Score: 194.1 bits (492), Expect = 1.5e-49
Identity = 92/167 (55.09%), Postives = 122/167 (73.05%), Query Frame = 0


Query: 5   KHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVN-RKAIQEVESFEEYSEPLL 64
           +HFVLVHG+CHGAWCWYK+K LLEA GHRVT +D+A +G++  ++I ++ + E+YSEPL 
Sbjct: 8   QHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPLT 67

Query: 65  KTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQFL 124
           K +  L  +EKV+LVGHSFGG++LA+AME FP KIS +VF+TAF+PDT H PS+VL++F 
Sbjct: 68  KLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKFG 127

Query: 125 ESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGE 171
            ++P+E WM TEF     D    S F F P  M   +YQLS  E  E
Sbjct: 128 SNMPQEAWMGTEFEPYGSDNSGLSMF-FSPDFMKLGLYQLSPVEDLE 173

[Close]

BLAST of CSPI06G27750 vs. TAIR 10
Match: AT2G23610.1 (methyl esterase 3 )

HSP 1 Score: 191.8 bits (486), Expect = 7.3e-49
Identity = 89/175 (50.86%), Postives = 126/175 (72.00%), Query Frame = 0


Query: 2   DQMKHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVN-RKAIQEVESFEEYSE 61
           ++ +H VLVHGACHGAWCWYK+K  LEA+GHRVT +D+A +G++  ++I ++ + E+YSE
Sbjct: 5   ERKQHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSE 64

Query: 62  PLLKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLE 121
           PL++ M  L  +EKV+LVGHS GG+SLA+AM+ FP KIS SVFVTA +PDT H PS+V +
Sbjct: 65  PLMQLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVWD 124

Query: 122 QFLESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGETSQTI 176
           +  +   RE W+DT F   + D  S  W +FGP+ MA  +YQLS  +  E ++ +
Sbjct: 125 KLRKETSREEWLDTVFTSEKPDFPSEFW-IFGPEFMAKNLYQLSPVQDLELAKML 178

[Close]

BLAST of CSPI06G27750 vs. TAIR 10
Match: AT2G23600.1 (acetone-cyanohydrin lyase )

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-48
Identity = 94/167 (56.29%), Postives = 120/167 (71.86%), Query Frame = 0


Query: 5   KHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVN-RKAIQEVESFEEYSEPLL 64
           +HFVLVHGACHGAWCWYK+K LLEA GHRVT LD+A +G++  ++I ++ + E+YSEPL+
Sbjct: 8   QHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPLM 67

Query: 65  KTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQFL 124
           + M  L  +EKV+LVGHSFGG+SLALAM+ FP KIS SVFVTAF+PDT H PS+V E+F 
Sbjct: 68  QLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKFA 127

Query: 125 ESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGE 171
            S+  E WM +E      D    S F F    M +++YQLS  E  E
Sbjct: 128 SSMTPEGWMGSELETYGSDNSGLSVF-FSTDFMKHRLYQLSPVEDLE 173

[Close]

BLAST of CSPI06G27750 vs. TAIR 10
Match: AT2G23590.1 (methyl esterase 8 )

HSP 1 Score: 177.2 bits (448), Expect = 1.9e-44
Identity = 83/166 (50.00%), Postives = 118/166 (71.08%), Query Frame = 0


Query: 5   KHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSEPLLK 64
           +HFVLVHG+C GAWCWYK+K LLEA+GHRVT LD+A  G++ ++I ++ + E+YSEPL++
Sbjct: 26  QHFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDLAACGIDTRSITDISTCEQYSEPLIQ 85

Query: 65  TMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQFLE 124
            M  L  +EKV+LVGHS+GG++LA+AM+ FP KIS SVFVT+F+PDT + PS+VLE+F  
Sbjct: 86  LMTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKISVSVFVTSFMPDTKNSPSFVLEKFAS 145

Query: 125 SLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGE 171
           ++  E WM +E            + +F  +   ++I QLS  E  E
Sbjct: 146 TMTPEDWMGSEL---------EPYVVFSAEFTKHRILQLSPIEDLE 182

[Close]

BLAST of CSPI06G27750 vs. TAIR 10
Match: AT2G23580.1 (methyl esterase 4 )

HSP 1 Score: 176.8 bits (447), Expect = 2.4e-44
Identity = 82/172 (47.67%), Postives = 119/172 (69.19%), Query Frame = 0


Query: 5   KHFVLVHGACHGAWCWYKIKLLLEAAGHRVTMLDMAGAGVNRKAIQEVESFEEYSEPLLK 64
           K FVLVHG CHGAWCWYK+K  LEA GH VT +D+A +G+N   ++E+++ ++Y +PLL+
Sbjct: 7   KRFVLVHGLCHGAWCWYKVKTHLEAVGHCVTAVDLAASGINMTRLEEIQTLKDYCKPLLE 66

Query: 65  TMACLG-PNEKVILVGHSFGGMSLALAMENFPHKISASVFVTAFVPDTHHPPSYVLEQFL 124
            +  LG  ++KVILV HS GG+  ALA + FP KI+  VF+TAF+PDT + P+YV ++ +
Sbjct: 67  LLNSLGSDDDKVILVAHSMGGIPAALASDIFPSKIATIVFLTAFMPDTRNLPAYVYQKLI 126

Query: 125 ESLPREFWMDTEFGENREDGGSSSWFLFGPKCMANKIYQLSHTESGETSQTI 176
            S+P+E W+DT FG   +      + LFGPK MA  +YQLS  +  E ++ +
Sbjct: 127 RSVPQEGWLDTVFGTYGKHECPLEFALFGPKFMAKNLYQLSPVQDLELAKML 178

The following BLAST results are available for this feature:

BLAST of CSPI06G27750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S8S9	2.1e-48	55.09	Methylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=MES1 PE=1 SV=1	[more]
O80477	1.0e-47	50.86	Methylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=MES3 PE=2 SV=1	[more]
O80476	1.8e-47	56.29	Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1	[more]
Q6RYA0	5.7e-46	52.69	Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1	[more]
Q9SE93	1.8e-44	48.00	Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=...	[more]

BLAST of CSPI06G27750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KG72	7.8e-91	96.41	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490...	[more]
A0A0A0KFL1	5.1e-90	96.41	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490...	[more]
A0A6J1IQY1	1.9e-68	73.99	salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11147763...	[more]
A0A6J1GDS1	2.7e-67	73.41	salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111453...	[more]
A0A5D3CMY2	1.1e-60	94.21	Methylesterase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of CSPI06G27750 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011657843.2	7.3e-91	97.01	salicylic acid-binding protein 2 [Cucumis sativus] >KAE8647492.1 hypothetical pr...	[more]
XP_004142018.1	1.1e-89	96.41	putative inactive methylesterase 20 [Cucumis sativus] >KGN48490.1 hypothetical p...	[more]
XP_038881260.1	1.3e-71	73.85	salicylic acid-binding protein 2-like isoform X1 [Benincasa hispida]	[more]
XP_022977269.1	3.9e-68	73.99	salicylic acid-binding protein 2-like [Cucurbita maxima]	[more]
XP_022949785.1	5.6e-67	73.41	salicylic acid-binding protein 2-like [Cucurbita moschata]	[more]

BLAST of CSPI06G27750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23620.1	1.5e-49	55.09	methyl esterase 1	[more]
AT2G23610.1	7.3e-49	50.86	methyl esterase 3	[more]
AT2G23600.1	1.3e-48	56.29	acetone-cyanohydrin lyase	[more]
AT2G23590.1	1.9e-44	50.00	methyl esterase 8	[more]
AT2G23580.1	2.4e-44	47.67	methyl esterase 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 6..130 e-value: 3.8E-17 score: 62.8
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 3..223 e-value: 4.7E-45 score: 156.4
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 6..172
None	No IPR available	PANTHER	PTHR10992:SF1027	METHYLESTERASE 1-RELATED	coord: 4..175
None	No IPR available	PANTHER	PTHR10992	METHYLESTERASE FAMILY MEMBER	coord: 4..175

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G27750.1	CSPI06G27750.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009694	jasmonic acid metabolic process
biological_process	GO:0009696	salicylic acid metabolic process
molecular_function	GO:0080030	methyl indole-3-acetate esterase activity
molecular_function	GO:0080032	methyl jasmonate esterase activity
molecular_function	GO:0080031	methyl salicylate esterase activity