CSPI06G26530 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G26530
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	cell wall / vacuolar inhibitor of fructosidase 2
Location	Chr6: 23397454 .. 23399061 (+)
RNA-Seq Expression	CSPI06G26530
Synteny	CSPI06G26530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATATAAATTTAGCTTTTCATCATTCCTCCTCACATTCTCTTCACAGTTCTTTCTTAATTATTCCTTCTCTCTCTCTCTCTCTTTTCCCTCAATCTCTCCAATTTATCCAATCTCGAGATCACTCTCATAAGCCTTAGCCTTGCAGAGATAGTTGTTTTTTTTCTTAATACAGATCAGTGATATATATCTTCTTCATTCTCTTCCCTAACAAGAACTCTCATATCTTCCTTTTCGTAACCCTATCTACCATTTGGAAAACACCAAAGAAATTAAACCTTCTCTTTTTCATCTCTCTCACTCAAAAAGAAGTAATTTGAGGTATTTTTTTTATATCATTTTTCTCTCATTTTGTCCTATGAACATTGTGAGATTTTTCTTTAGAATGTTATAAGTGTCATATCTTCTTCCATTGTCCCTCTCTATATCTCTCTCTTAACACCCAAAAAGAGAACTTGAGAGAGTGCCTTATTCATTATCCTCTCTCTCTCTCTCTCTCTCTCTCTTTCCCACATTCAGAAAAAGGATAGACTCTCATATGGATATATGAAGATCTTGCTTTTCTCCAATCTCTCTACCATTTAAAACAACCAAAAAGAAAAAAACCATCTCCTTCATCTCTCTCTCCAAAAAGAAGTAATCTTTGAGGAAAATCTCCAATGGGTATGAAGAATTTCTCTATTTCTCTCATTTTCTTTGCAATCCCATTAATCTTTTTCCACAAAAATAATGGAGTTTCATTAGCATCAGCTGACCAAACCCTAATCCAAAAAACATGCACAAACACTCTTTACTACAAACTTTGCATGTCATCCCTTAAATCCGACCCCGCCAGCCTCACCGCCGACACCAAAGGCCTTGCCGTCATCATGGCTTCCATCGGTGCTGCTAACGCCACCGCCACCGCCAGCTACCTCTCGTCTCAGCTCCCTACATCCTCCTCCGGTGCCGCTGCCAACAACAACAAAACAAAATTGCTGAGACAATGCTCCGAGAAATACGCATTTGCAGCTGAAGCACTTAGAGAATCTTTGAAAGATTTGGGTGATGAGACTTTTGACTATGCTTATATGCATGTTTCTGCGGCGGCGGATTATGCCAATGTTTGCCGTGATGCCTTTAAAGGGTTCCCAGCGGTGAGTTATCCGACGAAGCTTGGCCGGAGAGAAGAAGGGTTGAAGCGTATTTGCAGAGTGGTTTTGGGGATTTTGGATCTTCTTGGCTGGTGATTTTCATCAATCATTATGTTCTCTCTCTAATCATATTTTTGTTTTTCTTTTTTTTACTTTTACTTTTTGCTTTTACTTTTTTTTGGGGTAATAAACTTGTTTGTCTTTTTAGGTGAAACGTTGATGATGCTTTTCTTTAAGGGATTTAATGACTCCTTTATTTGTTAAAATAATTTATTTCTTCTACAGTAAGATCTCATGTGTTTGAATTAGGTTTAGTAATGACACTTTTGATGTTGTAAACTTATAGAGGATTTTATATTTTTGTCATTCTTTTTTTAAGTTTTGAGTTATGATGGATAATATATAATAGGTCGAATCAAAACCTCTTATCTATCTAAGTTGTAATATTTTTAATTTTGTATGTTCGAATTTTTTGATA

mRNA sequence

ATATAAATTTAGCTTTTCATCATTCCTCCTCACATTCTCTTCACAGTTCTTTCTTAATTATTCCTTCTCTCTCTCTCTCTCTTTTCCCTCAATCTCTCCAATTTATCCAATCTCGAGATCACTCTCATAAGCCTTAGCCTTGCAGAGATAGTTGTTTTTTTTCTTAATACAGATCAGTGATATATATCTTCTTCATTCTCTTCCCTAACAAGAACTCTCATATCTTCCTTTTCGTAACCCTATCTACCATTTGGAAAACACCAAAGAAATTAAACCTTCTCTTTTTCATCTCTCTCACTCAAAAAGAAGTAATTTGAGGTATTTTTTTTATATCATTTTTCTCTCATTTTGTCCTATGAACATTGTGAGATTTTTCTTTAGAATGTTATAAGTGTCATATCTTCTTCCATTGTCCCTCTCTATATCTCTCTCTTAACACCCAAAAAGAGAACTTGAGAGAGTGCCTTATTCATTATCCTCTCTCTCTCTCTCTCTCTCTCTCTTTCCCACATTCAGAAAAAGGATAGACTCTCATATGGATATATGAAGATCTTGCTTTTCTCCAATCTCTCTACCATTTAAAACAACCAAAAAGAAAAAAACCATCTCCTTCATCTCTCTCTCCAAAAAGAAGTAATCTTTGAGGAAAATCTCCAATGGGTATGAAGAATTTCTCTATTTCTCTCATTTTCTTTGCAATCCCATTAATCTTTTTCCACAAAAATAATGGAGTTTCATTAGCATCAGCTGACCAAACCCTAATCCAAAAAACATGCACAAACACTCTTTACTACAAACTTTGCATGTCATCCCTTAAATCCGACCCCGCCAGCCTCACCGCCGACACCAAAGGCCTTGCCGTCATCATGGCTTCCATCGGTGCTGCTAACGCCACCGCCACCGCCAGCTACCTCTCGTCTCAGCTCCCTACATCCTCCTCCGGTGCCGCTGCCAACAACAACAAAACAAAATTGCTGAGACAATGCTCCGAGAAATACGCATTTGCAGCTGAAGCACTTAGAGAATCTTTGAAAGATTTGGGTGATGAGACTTTTGACTATGCTTATATGCATGTTTCTGCGGCGGCGGATTATGCCAATGTTTGCCGTGATGCCTTTAAAGGGTTCCCAGCGGTGAGTTATCCGACGAAGCTTGGCCGGAGAGAAGAAGGGTTGAAGCGTATTTGCAGAGTGGTTTTGGGGATTTTGGATCTTCTTGGCTGGTGATTTTCATCAATCATTATGTTCTCTCTCTAATCATATTTTTGTTTTTCTTTTTTTTACTTTTACTTTTTGCTTTTACTTTTTTTTGGGGTAATAAACTTGTTTGTCTTTTTAGGTGAAACGTTGATGATGCTTTTCTTTAAGGGATTTAATGACTCCTTTATTTGTTAAAATAATTTATTTCTTCTACAGTAAGATCTCATGTGTTTGAATTAGGTTTAGTAATGACACTTTTGATGTTGTAAACTTATAGAGGATTTTATATTTTTGTCATTCTTTTTTTAAGTTTTGAGTTATGATGGATAATATATAATAGGTCGAATCAAAACCTCTTATCTATCTAAGTTGTAATATTTTTAATTTTGTATGTTCGAATTTTTTGATA

Coding sequence (CDS)

ATGGGTATGAAGAATTTCTCTATTTCTCTCATTTTCTTTGCAATCCCATTAATCTTTTTCCACAAAAATAATGGAGTTTCATTAGCATCAGCTGACCAAACCCTAATCCAAAAAACATGCACAAACACTCTTTACTACAAACTTTGCATGTCATCCCTTAAATCCGACCCCGCCAGCCTCACCGCCGACACCAAAGGCCTTGCCGTCATCATGGCTTCCATCGGTGCTGCTAACGCCACCGCCACCGCCAGCTACCTCTCGTCTCAGCTCCCTACATCCTCCTCCGGTGCCGCTGCCAACAACAACAAAACAAAATTGCTGAGACAATGCTCCGAGAAATACGCATTTGCAGCTGAAGCACTTAGAGAATCTTTGAAAGATTTGGGTGATGAGACTTTTGACTATGCTTATATGCATGTTTCTGCGGCGGCGGATTATGCCAATGTTTGCCGTGATGCCTTTAAAGGGTTCCCAGCGGTGAGTTATCCGACGAAGCTTGGCCGGAGAGAAGAAGGGTTGAAGCGTATTTGCAGAGTGGTTTTGGGGATTTTGGATCTTCTTGGCTGGTGA

Protein sequence

MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLLGW*

Homology

[Close]

BLAST of CSPI06G26530 vs. ExPASy Swiss-Prot
Match: O49603 (Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702 GN=C/VIF2 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-39
Identity = 89/179 (49.72%), Postives = 118/179 (65.92%), Query Frame = 0


Query: 9   SLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLA 68
           SLIF  +  + F  +  +S  S   T+I+ TC  T YYK C+S+LKSDP S TADTKGLA
Sbjct: 4   SLIFLLLVTLTFSASTLISAKSNTTTIIESTCKTTNYYKFCVSALKSDPRSPTADTKGLA 63

Query: 69  VIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLKDL 128
            IM  +G  NAT+TA+Y++  L    S    +    K+L+ CSEKYA AA++LR +++DL
Sbjct: 64  SIMVGVGMTNATSTANYIAGNL----SATVKDTVLKKVLQDCSEKYALAADSLRLTIQDL 123

Query: 129 GDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLL 188
            DE +DYA MHV AA DY NVCR+ F+    ++YP ++ RRE  L+RIC VV GILD L
Sbjct: 124 DDEAYDYASMHVLAAQDYPNVCRNIFRRVKGLAYPVEIRRREASLRRICGVVSGILDRL 178

[Close]

BLAST of CSPI06G26530 vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.2e-24
Identity = 63/165 (38.18%), Postives = 98/165 (59.39%), Query Frame = 0


Query: 26  VSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLAVIMASIGAANATA---T 85
           V +A     L++ TC +T YY +C+S+L +DP+S TAD +GL+ I  S+ AANA+     
Sbjct: 52  VPVAPQAAALVRATCNSTAYYDVCVSALAADPSSTTADVRGLSAIAVSVAAANASGAAQA 111

Query: 86  ASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLKDLGDETFDYAYMHVSA 145
           A+ L++     ++ AA +     LLR C+ KY  A +AL  + + +G + +D A +HVSA
Sbjct: 112 AAALANGTAPLAAAAAGDGTVQALLRACAGKYGDARDALAAAKESMGQQDYDLATVHVSA 171

Query: 146 AADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLL 188
            A+Y  VC+  F+     +YP +L  REE L ++C V L I+ LL
Sbjct: 172 GAEYPQVCKALFRRQRPGAYPAELAAREEALNKLCSVALDIIALL 216

[Close]

BLAST of CSPI06G26530 vs. ExPASy TrEMBL
Match: A0A0A0KJ03 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483430 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.8e-97
Identity = 188/189 (99.47%), Postives = 188/189 (99.47%), Query Frame = 0


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL
Sbjct: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGA ANNNKTKLLRQCSEKYAFAAEA
Sbjct: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV
Sbjct: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180

Query: 181 LGILDLLGW 190
           LGILDLLGW
Sbjct: 181 LGILDLLGW 189

[Close]

BLAST of CSPI06G26530 vs. ExPASy TrEMBL
Match: A0A1S3B228 (cell wall / vacuolar inhibitor of fructosidase 2 OS=Cucumis melo OX=3656 GN=LOC103485273 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 3.5e-90
Identity = 179/190 (94.21%), Postives = 184/190 (96.84%), Query Frame = 0


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNFSISLIFFAIPLIFFHK NGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDP+SL
Sbjct: 1   MGMKNFSISLIFFAIPLIFFHK-NGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPSSL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAA-ANNNKTKLLRQCSEKYAFAAE 120
           TADTKGLA+IMASIGAANATAT++YLSSQLPTSSS A  ANNNKTKLLRQCSEKYAFAAE
Sbjct: 61  TADTKGLAIIMASIGAANATATSTYLSSQLPTSSSAATNANNNKTKLLRQCSEKYAFAAE 120

Query: 121 ALRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRV 180
           ALRESLKDL DET+DYAYMHVSAAADYANVCRDAFKGFPAVSYP KLGRREEGLKRICRV
Sbjct: 121 ALRESLKDLADETYDYAYMHVSAAADYANVCRDAFKGFPAVSYPAKLGRREEGLKRICRV 180

Query: 181 VLGILDLLGW 190
           VLGILDLLGW
Sbjct: 181 VLGILDLLGW 189

[Close]

BLAST of CSPI06G26530 vs. ExPASy TrEMBL
Match: A0A6J1KU64 (cell wall / vacuolar inhibitor of fructosidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497231 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.7e-74
Identity = 149/189 (78.84%), Postives = 162/189 (85.71%), Query Frame = 0


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MG+KNF I L+F A P IF H+       +ADQTLIQKTC NTLYY LCMSSLKSDP SL
Sbjct: 1   MGLKNFLIFLLFLANPTIFLHQT--TKFVAADQTLIQKTCRNTLYYDLCMSSLKSDPTSL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLA IM SIG +NATATA+YLS+QLPT S+GAAANNN+TKLLRQCSEKY FAAEA
Sbjct: 61  TADTKGLAAIMVSIGVSNATATATYLSTQLPT-SAGAAANNNRTKLLRQCSEKYGFAAEA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           LR SLKDL +ET+DYAYMHVSAAADYANVC D FKGFP V+YP KLGRREEGLKRICRVV
Sbjct: 121 LRASLKDLAEETYDYAYMHVSAAADYANVCHDGFKGFPTVAYPAKLGRREEGLKRICRVV 180

Query: 181 LGILDLLGW 190
           LGILD+LGW
Sbjct: 181 LGILDVLGW 186

[Close]

BLAST of CSPI06G26530 vs. ExPASy TrEMBL
Match: A0A6J1HHS2 (cell wall / vacuolar inhibitor of fructosidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111463098 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.7e-74
Identity = 150/189 (79.37%), Postives = 161/189 (85.19%), Query Frame = 0


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNF I L+F A P IF H+       +ADQTLIQKTC NTLYY LCMSSLKSDP SL
Sbjct: 1   MGMKNFLIFLLFLANPSIFLHQT--TKFVAADQTLIQKTCRNTLYYDLCMSSLKSDPTSL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLA IM SIG +NATATA+YLS+QLPT S+GAAANNN+TKLLRQCSEKY FAAEA
Sbjct: 61  TADTKGLAAIMVSIGVSNATATATYLSTQLPT-SAGAAANNNRTKLLRQCSEKYGFAAEA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           LR SLKDL +ET+DYAYMHVSAAADYANVC D FKGFP V+YP KLGRREEGLKRICRVV
Sbjct: 121 LRASLKDLAEETYDYAYMHVSAAADYANVCHDGFKGFPTVAYPAKLGRREEGLKRICRVV 180

Query: 181 LGILDLLGW 190
           LGILD LGW
Sbjct: 181 LGILDALGW 186

[Close]

BLAST of CSPI06G26530 vs. ExPASy TrEMBL
Match: A0A6J1GFG5 (cell wall / vacuolar inhibitor of fructosidase 2 OS=Cucurbita moschata OX=3662 GN=LOC111453451 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.2e-66
Identity = 137/189 (72.49%), Postives = 155/189 (82.01%), Query Frame = 0


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKN  +        L+F +     +  +ADQTLIQKTC +TLYY LCMSSLKSDP SL
Sbjct: 1   MGMKNLGM--------LLFLYLVTPTTFVAADQTLIQKTCRSTLYYALCMSSLKSDPTSL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLA IMASIGAANATAT++YLSSQLPTS++    N+N+TKL+RQCSEKYAFAAEA
Sbjct: 61  TADTKGLAAIMASIGAANATATSTYLSSQLPTSANNNKNNSNRTKLIRQCSEKYAFAAEA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           LR SLKDL +ET+DYAYMHV+AAADYANVCR+ FK FP V+YP  L RREEGLKRICRVV
Sbjct: 121 LRASLKDLAEETYDYAYMHVAAAADYANVCRNGFKVFPTVAYPATLRRREEGLKRICRVV 180

Query: 181 LGILDLLGW 190
           LGILDLLGW
Sbjct: 181 LGILDLLGW 181

[Close]

BLAST of CSPI06G26530 vs. NCBI nr
Match: XP_004148650.1 (cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus] >KGN48362.1 hypothetical protein Csa_003804 [Cucumis sativus])

HSP 1 Score: 364.0 bits (933), Expect = 7.9e-97
Identity = 188/189 (99.47%), Postives = 188/189 (99.47%), Query Frame = 0


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL
Sbjct: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGA ANNNKTKLLRQCSEKYAFAAEA
Sbjct: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAGANNNKTKLLRQCSEKYAFAAEA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV
Sbjct: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180

Query: 181 LGILDLLGW 190
           LGILDLLGW
Sbjct: 181 LGILDLLGW 189

[Close]

BLAST of CSPI06G26530 vs. NCBI nr
Match: XP_008441045.1 (PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis melo])

HSP 1 Score: 340.9 bits (873), Expect = 7.1e-90
Identity = 179/190 (94.21%), Postives = 184/190 (96.84%), Query Frame = 0


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNFSISLIFFAIPLIFFHK NGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDP+SL
Sbjct: 1   MGMKNFSISLIFFAIPLIFFHK-NGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPSSL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAA-ANNNKTKLLRQCSEKYAFAAE 120
           TADTKGLA+IMASIGAANATAT++YLSSQLPTSSS A  ANNNKTKLLRQCSEKYAFAAE
Sbjct: 61  TADTKGLAIIMASIGAANATATSTYLSSQLPTSSSAATNANNNKTKLLRQCSEKYAFAAE 120

Query: 121 ALRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRV 180
           ALRESLKDL DET+DYAYMHVSAAADYANVCRDAFKGFPAVSYP KLGRREEGLKRICRV
Sbjct: 121 ALRESLKDLADETYDYAYMHVSAAADYANVCRDAFKGFPAVSYPAKLGRREEGLKRICRV 180

Query: 181 VLGILDLLGW 190
           VLGILDLLGW
Sbjct: 181 VLGILDLLGW 189

[Close]

BLAST of CSPI06G26530 vs. NCBI nr
Match: XP_038882809.1 (cell wall / vacuolar inhibitor of fructosidase 2 [Benincasa hispida])

HSP 1 Score: 315.8 bits (808), Expect = 2.5e-82
Identity = 163/189 (86.24%), Postives = 172/189 (91.01%), Query Frame = 0


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMK FSI L++ AIPLIFFHKN  VSL +ADQTLIQKTCTNTLYY LCMSSLKSDP SL
Sbjct: 1   MGMKKFSIFLLYLAIPLIFFHKNE-VSLVAADQTLIQKTCTNTLYYNLCMSSLKSDPTSL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLA IMASIGA NATATA+YLSSQLPTS+S AA NNNKTKL+RQCSEKY FAAEA
Sbjct: 61  TADTKGLAAIMASIGATNATATATYLSSQLPTSASTAANNNNKTKLIRQCSEKYGFAAEA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           LR SLKDL DET+DYAYMHVSAAADYANVC DAF+GFPAV+YP KLGRREEGLKRICRVV
Sbjct: 121 LRASLKDLADETYDYAYMHVSAAADYANVCHDAFRGFPAVTYPAKLGRREEGLKRICRVV 180

Query: 181 LGILDLLGW 190
           LGILDLLGW
Sbjct: 181 LGILDLLGW 188

[Close]

BLAST of CSPI06G26530 vs. NCBI nr
Match: KAG6595125.1 (Cell wall / vacuolar inhibitor of fructosidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 288.9 bits (738), Expect = 3.2e-74
Identity = 150/189 (79.37%), Postives = 162/189 (85.71%), Query Frame = 0


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNF I L+F A P IF H+       +ADQTLIQKTC NTLYY LCMSSLKSDP SL
Sbjct: 1   MGMKNFLIFLLFLANPSIFLHQT--TKFVAADQTLIQKTCRNTLYYDLCMSSLKSDPTSL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLA IM SIG +NATATA+YLS+QLPT S+GAAANNN+TKLLRQCSEKY FAAEA
Sbjct: 61  TADTKGLAAIMVSIGVSNATATATYLSTQLPT-STGAAANNNRTKLLRQCSEKYGFAAEA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           LR SLKDL +ET+DYAYMHVSAAADYANVC D FKGFP V+YP KLGRREEGLKRICRVV
Sbjct: 121 LRASLKDLAEETYDYAYMHVSAAADYANVCHDGFKGFPTVAYPAKLGRREEGLKRICRVV 180

Query: 181 LGILDLLGW 190
           LGILD+LGW
Sbjct: 181 LGILDVLGW 186

[Close]

BLAST of CSPI06G26530 vs. NCBI nr
Match: KAG7027144.1 (Cell wall / vacuolar inhibitor of fructosidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 288.9 bits (738), Expect = 3.2e-74
Identity = 150/189 (79.37%), Postives = 162/189 (85.71%), Query Frame = 0


Query: 1   MGMKNFSISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASL 60
           MGMKNF I L+F A P IF H+       +ADQTLIQKTC NTLYY LCMSSLKSDP SL
Sbjct: 1   MGMKNFLIFLLFLANPSIFLHQT--TKFVAADQTLIQKTCRNTLYYDLCMSSLKSDPTSL 60

Query: 61  TADTKGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEA 120
           TADTKGLA IM SIG +NATATA+YLS+QLPT S+GAAANNN+TKLLRQCSEKY FAAEA
Sbjct: 61  TADTKGLAAIMVSIGVSNATATATYLSTQLPT-SAGAAANNNRTKLLRQCSEKYGFAAEA 120

Query: 121 LRESLKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVV 180
           LR SLKDL +ET+DYAYMHVSAAADYANVC D FKGFP V+YP KLGRREEGLKRICRVV
Sbjct: 121 LRASLKDLAEETYDYAYMHVSAAADYANVCHDGFKGFPTVAYPAKLGRREEGLKRICRVV 180

Query: 181 LGILDLLGW 190
           LGILD+LGW
Sbjct: 181 LGILDVLGW 186

[Close]

BLAST of CSPI06G26530 vs. TAIR 10
Match: AT5G64620.1 (cell wall / vacuolar inhibitor of fructosidase 2 )

HSP 1 Score: 164.5 bits (415), Expect = 8.5e-41
Identity = 89/179 (49.72%), Postives = 118/179 (65.92%), Query Frame = 0


Query: 9   SLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLA 68
           SLIF  +  + F  +  +S  S   T+I+ TC  T YYK C+S+LKSDP S TADTKGLA
Sbjct: 4   SLIFLLLVTLTFSASTLISAKSNTTTIIESTCKTTNYYKFCVSALKSDPRSPTADTKGLA 63

Query: 69  VIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLKDL 128
            IM  +G  NAT+TA+Y++  L    S    +    K+L+ CSEKYA AA++LR +++DL
Sbjct: 64  SIMVGVGMTNATSTANYIAGNL----SATVKDTVLKKVLQDCSEKYALAADSLRLTIQDL 123

Query: 129 GDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGILDLL 188
            DE +DYA MHV AA DY NVCR+ F+    ++YP ++ RRE  L+RIC VV GILD L
Sbjct: 124 DDEAYDYASMHVLAAQDYPNVCRNIFRRVKGLAYPVEIRRREASLRRICGVVSGILDRL 178

[Close]

BLAST of CSPI06G26530 vs. TAIR 10
Match: AT1G55770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 44.3 bits (103), Expect = 1.3e-04
Identity = 47/183 (25.68%), Postives = 77/183 (42.08%), Query Frame = 0


Query: 7   SISLIFFAIPLIFFHKNNGVSLASADQTLIQKTCTNTLYYKLCMSSLKSDPAS--LTADT 66
           S  ++F  I LI F   N      A+  +I   C ++    LC+SS+ S P S      +
Sbjct: 4   SAGVLFLCIVLISFVTGN------ANSGMISDLCKHSDDPNLCLSSITSRPESGEFAGTS 63

Query: 67  KGLAVIMASIGAANATATASYLSSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRES 126
             + +I  S  +ANA+AT+SY+  +L       A  +     L  C + Y  A E L +S
Sbjct: 64  NQIEIIAISAASANASATSSYIKQKLSNEDLEPAIEDT----LEDCQKDYQDAVEQLDDS 123

Query: 127 LKDLGDETFDYAYMHVSAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKRICRVVLGIL 186
           +  +  +      + +SAA      C  A           +L +R E   ++C+  L I 
Sbjct: 124 ISAMLADAHTDVDVWLSAAISAIESCGSALGS--RAGNDAELSQRNEVFLKLCKNALMIN 174

Query: 187 DLL 188
            +L
Sbjct: 184 KML 174

[Close]

BLAST of CSPI06G26530 vs. TAIR 10
Match: AT1G23205.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 1.7e-04
Identity = 48/190 (25.26%), Postives = 78/190 (41.05%), Query Frame = 0


Query: 16  PLIFFHKNNGVSL------ASADQTLIQKTCTNTLYYKLCMSSLKSDPASLTADTKGLAV 75
           PL+FF   + + L       S D   I+ +C  TLY  LC SSL S  +S+  D   LA 
Sbjct: 8   PLLFFFLASVLPLILTVHSQSDDSDFIRTSCNTTLYPDLCFSSLSSFSSSVHNDPALLAR 67

Query: 76  IMASIGAANATATASYL---SSQLPTSSSGAAANNNKTKLLRQCSEKYAFAAEALRESLK 135
              S+        ASYL   ++  P S+   A +     +   C +    A E ++ S+K
Sbjct: 68  AAISVTLTKTLDLASYLANITTLQPESNEDGAHHPTAAAVFHDCFDNLKDAVEEMKGSMK 127

Query: 136 DLGD-------ETFDYAYMHV----SAAADYANVCRDAFKGFPAVSYPTKLGRREEGLKR 186
            + +       E+F +   +V    SAA      C D FK          +  R + +K+
Sbjct: 128 QMRELVSTGSLESFRFQMSNVQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKK 187

The following BLAST results are available for this feature:

BLAST of CSPI06G26530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49603	1.2e-39	49.72	Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702...	[more]
Q0J8J8	3.2e-24	38.18	Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 P...	[more]

BLAST of CSPI06G26530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KJ03	3.8e-97	99.47	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483430 PE=4 S...	[more]
A0A1S3B228	3.5e-90	94.21	cell wall / vacuolar inhibitor of fructosidase 2 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1KU64	2.7e-74	78.84	cell wall / vacuolar inhibitor of fructosidase 2-like OS=Cucurbita maxima OX=366...	[more]
A0A6J1HHS2	2.7e-74	79.37	cell wall / vacuolar inhibitor of fructosidase 2-like OS=Cucurbita moschata OX=3...	[more]
A0A6J1GFG5	1.2e-66	72.49	cell wall / vacuolar inhibitor of fructosidase 2 OS=Cucurbita moschata OX=3662 G...	[more]

BLAST of CSPI06G26530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148650.1	7.9e-97	99.47	cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus] >KGN48362.1 h...	[more]
XP_008441045.1	7.1e-90	94.21	PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis melo]	[more]
XP_038882809.1	2.5e-82	86.24	cell wall / vacuolar inhibitor of fructosidase 2 [Benincasa hispida]	[more]
KAG6595125.1	3.2e-74	79.37	Cell wall / vacuolar inhibitor of fructosidase 2, partial [Cucurbita argyrosperm...	[more]
KAG7027144.1	3.2e-74	79.37	Cell wall / vacuolar inhibitor of fructosidase 2, partial [Cucurbita argyrosperm...	[more]

BLAST of CSPI06G26530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G64620.1	8.5e-41	49.72	cell wall / vacuolar inhibitor of fructosidase 2	[more]
AT1G55770.1	1.3e-04	25.68	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G23205.1	1.7e-04	25.26	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 31..183 e-value: 1.0E-28 score: 111.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 5..186 e-value: 7.6E-35 score: 117.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 35..183 e-value: 2.2E-27 score: 96.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 30..187 e-value: 4.0E-33 score: 116.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 33..187
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 24..187
None	No IPR available	PANTHER	PTHR31080:SF152	CELL WALL / VACUOLAR INHIBITOR OF FRUCTOSIDASE 2	coord: 24..187
None	No IPR available	CDD	cd14859	PMEI_like	coord: 40..186 e-value: 1.75338E-34 score: 117.147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G26530.1	CSPI06G26530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity