CSPI06G26320 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G26320
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCysteine proteinase inhibitor 5-like
LocationChr6: 23273241 .. 23273582 (-)
RNA-Seq ExpressionCSPI06G26320
SyntenyCSPI06G26320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAATAAGTTTGTCAATATTACAAGTAAGTAAAAGGTCTAAAATGGCTTCTGATCCAGTGTCCAACGTCTATAGTCCAGTTGAGGATCCTCAAAGTCAACGCATGAAAGAATTAGCAGAATGGATAGTAGCAGAGCACAACAAAAACGAAGGGACGCACTTGAAGTTCATTCGTATATGGAAGTGTGAGGTGCAAATTGTGAATGGAGTCAACCACCGCTTCACTTTGACGGCTAAGGATGAAAACGATTATGAAGCTGCATACATGGCTGTTGTCTTGGAGCAACAATGGAAGCATCTCAAGGAGCTCGTTTACTTTAAGAAACTCTTCTTGGCCGAATAA

mRNA sequence

GTAATAAGTTTGTCAATATTACAAGTAAGTAAAAGGTCTAAAATGGCTTCTGATCCAGTGTCCAACGTCTATAGTCCAGTTGAGGATCCTCAAAGTCAACGCATGAAAGAATTAGCAGAATGGATAGTAGCAGAGCACAACAAAAACGAAGGGACGCACTTGAAGTTCATTCGTATATGGAAGTGTGAGGTGCAAATTGTGAATGGAGTCAACCACCGCTTCACTTTGACGGCTAAGGATGAAAACGATTATGAAGCTGCATACATGGCTGTTGTCTTGGAGCAACAATGGAAGCATCTCAAGGAGCTCGTTTACTTTAAGAAACTCTTCTTGGCCGAATAA

Coding sequence (CDS)

ATGGCTTCTGATCCAGTGTCCAACGTCTATAGTCCAGTTGAGGATCCTCAAAGTCAACGCATGAAAGAATTAGCAGAATGGATAGTAGCAGAGCACAACAAAAACGAAGGGACGCACTTGAAGTTCATTCGTATATGGAAGTGTGAGGTGCAAATTGTGAATGGAGTCAACCACCGCTTCACTTTGACGGCTAAGGATGAAAACGATTATGAAGCTGCATACATGGCTGTTGTCTTGGAGCAACAATGGAAGCATCTCAAGGAGCTCGTTTACTTTAAGAAACTCTTCTTGGCCGAATAA

Protein sequence

MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRFTLTAKDENDYEAAYMAVVLEQQWKHLKELVYFKKLFLAE*
Homology
BLAST of CSPI06G26320 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 1.9e-07
Identity = 27/86 (31.40%), Postives = 49/86 (56.98%), Query Frame = 0

Query: 10  YSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRFTLTAKDEND 69
           + P+E+  S  ++++A++ V+EHNK     L++  + +   Q+V G N+R  + AKD   
Sbjct: 32  WRPIENLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLVIAAKD-GA 91

Query: 70  YEAAYMAVVLEQQWKHLKELVYFKKL 96
               Y AVV ++ W H + L  F+K+
Sbjct: 92  VVGNYEAVVWDKPWMHFRNLTSFRKV 116

BLAST of CSPI06G26320 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 2.4e-07
Identity = 27/86 (31.40%), Postives = 48/86 (55.81%), Query Frame = 0

Query: 10  YSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRFTLTAKDEND 69
           + P+E   S  ++++A++ V+EHNK     L++  + +   Q+V G N+R  + AKD   
Sbjct: 32  WRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLVIAAKD-GA 91

Query: 70  YEAAYMAVVLEQQWKHLKELVYFKKL 96
               Y AVV ++ W H + L  F+K+
Sbjct: 92  VVGNYEAVVWDKPWMHFRNLTSFRKV 116

BLAST of CSPI06G26320 vs. ExPASy Swiss-Prot
Match: Q84WT8 (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 6.0e-06
Identity = 26/84 (30.95%), Postives = 44/84 (52.38%), Query Frame = 0

Query: 12  PVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRFTLTAKDENDYE 71
           P+++     +  +A++ + EHNK     L F+++ +   Q+V+G  +   + AKD     
Sbjct: 34  PIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTKYDLKIAAKDGGGKI 93

Query: 72  AAYMAVVLEQQWKHLKELVYFKKL 96
             Y AVV+E+ W H K L  FK L
Sbjct: 94  KNYEAVVVEKLWLHSKSLESFKAL 117

BLAST of CSPI06G26320 vs. ExPASy Swiss-Prot
Match: P20907 (Cysteine proteinase inhibitor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0494200 PE=2 SV=3)

HSP 1 Score: 50.8 bits (120), Expect = 1.0e-05
Identity = 27/73 (36.99%), Postives = 37/73 (50.68%), Query Frame = 0

Query: 23  ELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRFTLTAKDENDYEAAYMAVVLEQQ 82
           ELA + VAEHN      L+  R+ K   Q+V G  H  T+  K+       Y A V E+ 
Sbjct: 74  ELARFAVAEHNSKANAMLELERVVKVRQQVVGGFMHYLTVEVKEPGGANKLYEAKVWERA 133

Query: 83  WKHLKELVYFKKL 96
           W++ K+L  FK L
Sbjct: 134 WENFKQLQDFKPL 146

BLAST of CSPI06G26320 vs. ExPASy Swiss-Prot
Match: Q9LEI7 (Cysteine proteinase inhibitor OS=Hordeum vulgare OX=4513 GN=ICY PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 3.0e-05
Identity = 28/71 (39.44%), Postives = 39/71 (54.93%), Query Frame = 0

Query: 23  ELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRFTLTAKDENDYEAAYMAVVLEQQ 82
           ELA + VAEHN      L+F ++ K   Q+V G  H FT+  K E   +  Y A V E+ 
Sbjct: 35  ELARFAVAEHNAKANALLEFEKLVKVRQQVVAGCMHYFTIEVK-EGGAKKLYEAKVWEKA 94

Query: 83  WKHLKELVYFK 94
           W++ K+L  FK
Sbjct: 95  WENFKQLQEFK 104

BLAST of CSPI06G26320 vs. ExPASy TrEMBL
Match: A0A0A0KKR3 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483247 PE=4 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 7.0e-50
Identity = 99/99 (100.00%), Postives = 99/99 (100.00%), Query Frame = 0

Query: 1   MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRF 60
           MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRF
Sbjct: 1   MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRF 60

Query: 61  TLTAKDENDYEAAYMAVVLEQQWKHLKELVYFKKLFLAE 100
           TLTAKDENDYEAAYMAVVLEQQWKHLKELVYFKKLFLAE
Sbjct: 61  TLTAKDENDYEAAYMAVVLEQQWKHLKELVYFKKLFLAE 99

BLAST of CSPI06G26320 vs. ExPASy TrEMBL
Match: A0A5D3DPT6 (Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1230G00020 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.7e-44
Identity = 90/99 (90.91%), Postives = 93/99 (93.94%), Query Frame = 0

Query: 1   MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRF 60
           MASD VSN+YSPVEDPQSQRMKELAEW VAEHNK EGTHLKFIRI KCEVQIVNGVNHRF
Sbjct: 1   MASDLVSNIYSPVEDPQSQRMKELAEWTVAEHNKKEGTHLKFIRIMKCEVQIVNGVNHRF 60

Query: 61  TLTAKDENDYEAAYMAVVLEQQWKHLKELVYFKKLFLAE 100
           TLTAKDENDYEA YMAVVLEQQWKHLKELVYF++L LAE
Sbjct: 61  TLTAKDENDYEAGYMAVVLEQQWKHLKELVYFRQLLLAE 99

BLAST of CSPI06G26320 vs. ExPASy TrEMBL
Match: A0A0A0KF22 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G473520 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.3e-29
Identity = 71/101 (70.30%), Postives = 79/101 (78.22%), Query Frame = 0

Query: 1   MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRF 60
           MASD V   Y+PVE+PQS RMKELAEW VAEHNK  GTHL FI I  CE QIV+GVN+RF
Sbjct: 1   MASDLVPGGYTPVENPQSSRMKELAEWAVAEHNKKAGTHLMFIGILTCESQIVDGVNYRF 60

Query: 61  TLTAKDEND--YEAAYMAVVLEQQWKHLKELVYFKKLFLAE 100
           TLTAKDE D     +YMAVV EQ W+H+KELVYF+KL LAE
Sbjct: 61  TLTAKDEKDNCEIESYMAVVFEQPWEHIKELVYFQKLLLAE 101

BLAST of CSPI06G26320 vs. ExPASy TrEMBL
Match: A0A0A0KF46 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483245 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 4.4e-28
Identity = 67/92 (72.83%), Postives = 76/92 (82.61%), Query Frame = 0

Query: 10  YSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRFTLTAKDEND 69
           Y+PV+DPQS RMKELAEW VAEHNK EGTHL+FI I KCE QIVNGVN+RFTLTAKDE+D
Sbjct: 9   YTPVKDPQSPRMKELAEWAVAEHNKKEGTHLRFISILKCESQIVNGVNYRFTLTAKDESD 68

Query: 70  --YEAAYMAVVLEQQWKHLKELVYFKKLFLAE 100
                 YMA+V EQ  +HLKELV+FK+L LAE
Sbjct: 69  NCLPGNYMAIVYEQLSEHLKELVFFKQLLLAE 100

BLAST of CSPI06G26320 vs. ExPASy TrEMBL
Match: A0A5A7TGI6 (Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4249G00020 PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 4.1e-26
Identity = 68/103 (66.02%), Postives = 78/103 (75.73%), Query Frame = 0

Query: 1   MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRF 60
           MASD VS  Y+PVE+PQS RMKELAEW +AEHNK EGTHL+FI I  CE QIVNGVN+RF
Sbjct: 1   MASDIVSGGYTPVENPQSPRMKELAEWAIAEHNKKEGTHLRFISILTCESQIVNGVNYRF 60

Query: 61  TLTAKDENDYE----AAYMAVVLEQQWKHLKELVYFKKLFLAE 100
           TLTAK  NDY+      YMA+V EQ  +  KELV+FK+L L E
Sbjct: 61  TLTAK--NDYDNCLPGKYMAIVYEQPSECFKELVFFKQLLLDE 101

BLAST of CSPI06G26320 vs. NCBI nr
Match: KAE8647458.1 (hypothetical protein Csa_004041 [Cucumis sativus])

HSP 1 Score: 188.0 bits (476), Expect = 4.1e-44
Identity = 89/90 (98.89%), Postives = 90/90 (100.00%), Query Frame = 0

Query: 1  MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRF 60
          MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRF
Sbjct: 1  MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRF 60

Query: 61 TLTAKDENDYEAAYMAVVLEQQWKHLKELV 91
          TLTAKDENDYEAAYMAVVLEQQWKHLKEL+
Sbjct: 61 TLTAKDENDYEAAYMAVVLEQQWKHLKELM 90

BLAST of CSPI06G26320 vs. NCBI nr
Match: KAA0025518.1 (cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa] >TYK25673.1 cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 186.4 bits (472), Expect = 1.2e-43
Identity = 90/99 (90.91%), Postives = 93/99 (93.94%), Query Frame = 0

Query: 1   MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRF 60
           MASD VSN+YSPVEDPQSQRMKELAEW VAEHNK EGTHLKFIRI KCEVQIVNGVNHRF
Sbjct: 1   MASDLVSNIYSPVEDPQSQRMKELAEWTVAEHNKKEGTHLKFIRIMKCEVQIVNGVNHRF 60

Query: 61  TLTAKDENDYEAAYMAVVLEQQWKHLKELVYFKKLFLAE 100
           TLTAKDENDYEA YMAVVLEQQWKHLKELVYF++L LAE
Sbjct: 61  TLTAKDENDYEAGYMAVVLEQQWKHLKELVYFRQLLLAE 99

BLAST of CSPI06G26320 vs. NCBI nr
Match: KAA0040697.1 (cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa] >TYK07890.1 cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 127.1 bits (318), Expect = 8.5e-26
Identity = 68/103 (66.02%), Postives = 78/103 (75.73%), Query Frame = 0

Query: 1   MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRF 60
           MASD VS  Y+PVE+PQS RMKELAEW +AEHNK EGTHL+FI I  CE QIVNGVN+RF
Sbjct: 1   MASDIVSGGYTPVENPQSPRMKELAEWAIAEHNKKEGTHLRFISILTCESQIVNGVNYRF 60

Query: 61  TLTAKDENDYE----AAYMAVVLEQQWKHLKELVYFKKLFLAE 100
           TLTAK  NDY+      YMA+V EQ  +  KELV+FK+L L E
Sbjct: 61  TLTAK--NDYDNCLPGKYMAIVYEQPSECFKELVFFKQLLLDE 101

BLAST of CSPI06G26320 vs. NCBI nr
Match: XP_008441065.1 (PREDICTED: cysteine proteinase inhibitor 5-like [Cucumis melo] >KAA0025519.1 cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 123.6 bits (309), Expect = 9.4e-25
Identity = 62/101 (61.39%), Postives = 74/101 (73.27%), Query Frame = 0

Query: 1   MASDPVSNV--YSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNH 60
           MASDPV     Y+PV+DPQS  MKELAEW VAE+NK EGTHL+F+ I +CE QIV GVN+
Sbjct: 1   MASDPVPGAGGYTPVKDPQSPHMKELAEWAVAEYNKKEGTHLRFVCILECESQIVEGVNY 60

Query: 61  RFTLTAKDENDYEAAYMAVVLEQQWKHLKELVYFKKLFLAE 100
           RF L A DE   E  Y A+V E+ W+  KELVYFK+L L+E
Sbjct: 61  RFILKANDEKANEGNYEAIVWEKTWEDFKELVYFKQLLLSE 101

BLAST of CSPI06G26320 vs. NCBI nr
Match: KAE8645936.1 (hypothetical protein Csa_021372 [Cucumis sativus])

HSP 1 Score: 123.2 bits (308), Expect = 1.2e-24
Identity = 63/91 (69.23%), Postives = 70/91 (76.92%), Query Frame = 0

Query: 1  MASDPVSNVYSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRF 60
          MASD V   Y+PVE+PQS RMKELAEW VAEHNK  GTHL FI I  CE QIV+GVN+RF
Sbjct: 1  MASDLVPGGYTPVENPQSSRMKELAEWAVAEHNKKAGTHLMFIGILTCESQIVDGVNYRF 60

Query: 61 TLTAKDEND--YEAAYMAVVLEQQWKHLKEL 90
          TLTAKDE D     +YMAVV EQ W+H+KEL
Sbjct: 61 TLTAKDEKDNCEIESYMAVVFEQPWEHIKEL 91

BLAST of CSPI06G26320 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 4.2e-07
Identity = 26/84 (30.95%), Postives = 44/84 (52.38%), Query Frame = 0

Query: 12  PVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRFTLTAKDENDYE 71
           P+++     +  +A++ + EHNK     L F+++ +   Q+V+G  +   + AKD     
Sbjct: 34  PIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTKYDLKIAAKDGGGKI 93

Query: 72  AAYMAVVLEQQWKHLKELVYFKKL 96
             Y AVV+E+ W H K L  FK L
Sbjct: 94  KNYEAVVVEKLWLHSKSLESFKAL 117

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P864721.9e-0731.40Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841... [more]
Q6TPK42.4e-0731.40Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
Q84WT86.0e-0630.95Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=... [more]
P209071.0e-0536.99Cysteine proteinase inhibitor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05... [more]
Q9LEI73.0e-0539.44Cysteine proteinase inhibitor OS=Hordeum vulgare OX=4513 GN=ICY PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KKR37.0e-50100.00Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483247 PE... [more]
A0A5D3DPT65.7e-4490.91Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A0A0KF222.3e-2970.30Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G473520 PE... [more]
A0A0A0KF464.4e-2872.83Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483245 PE... [more]
A0A5A7TGI64.1e-2666.02Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
KAE8647458.14.1e-4498.89hypothetical protein Csa_004041 [Cucumis sativus][more]
KAA0025518.11.2e-4390.91cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa] >TYK25673.1 cyst... [more]
KAA0040697.18.5e-2666.02cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa] >TYK07890.1 cyst... [more]
XP_008441065.19.4e-2561.39PREDICTED: cysteine proteinase inhibitor 5-like [Cucumis melo] >KAA0025519.1 cys... [more]
KAE8645936.11.2e-2469.23hypothetical protein Csa_021372 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT4G16500.14.2e-0730.95Cystatin/monellin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainSMARTSM00043CY_4coord: 8..95
e-value: 0.005
score: 24.4
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 15..94
e-value: 2.1E-20
score: 72.8
IPR000010Cystatin domainCDDcd00042CYcoord: 22..93
e-value: 1.22103E-5
score: 38.6092
NoneNo IPR availableGENE3D3.10.450.10coord: 3..96
e-value: 5.0E-22
score: 79.9
NoneNo IPR availablePANTHERPTHR47364CYSTEINE PROTEINASE INHIBITOR 5coord: 3..95
NoneNo IPR availablePANTHERPTHR47364:SF2CYSTEINE PROTEINASE INHIBITOR 5coord: 3..95
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 7..93

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G26320.1CSPI06G26320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity