CSPI06G25760 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G25760
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Lipid droplet-associated hydrolase
Location	Chr6: 22864719 .. 22869763 (-)
RNA-Seq Expression	CSPI06G25760
Synteny	CSPI06G25760

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATTTTTAAAAAACCAATTATAAAAAACGAAATGGTTTATCATTGGGGGAATCTTGATATTTATTAAAAATCAACAGATGGATTCATTATGATCCATACTCGAAAGAGAACATGAATTGCGGGGATTTCGAGAGCCGTAGAAGAATCCATGTTCTTACCGCTTCCTTCACTCACCGTCCGACCTACCCTTCTCATATCATATTCATATTCCAGGTATTTCACTTCCATTCTTCATAATTTCATTACTTTAATCTTTAACTTTTCATTACCCATTTTGTTTATTATATTCAATTCAACGTGCAGTGTAATGTTCCCAAGTTTTGCATCACAGTGATGCACCATAGTCAACACACTTCTCCATTTTACCGCCAATTCACTTGGAACATCCCATCCCATTTCATCCATTCTTTTGTTCCCATTTTAATCTTTCAGGTTGAAGAATTTCTCTAGGTGTTCGTTTCTCCATATGGGTCATCAAGTTCCGCAATCTTTTAGTAAAAGGCGTGTGGAGTTTCGTTTATGCAATGTATCGGGGTAATTGATTCATTCCCGTTTATATTCACTGAACTTCTCTTGGAATTGCGTTTTATTTGACTTTCCATTTCTGTGTTTTGTTAGTTTTACCAATGAATTACTGGAGATTCACTCGGATGATCCATCATTGCACGTATTGTTTATTCCTGGAAATCCAGGTGCGTTTGCGTTTTGGGGATGTGGGTTTTTTTTTTATTCTTCTGTTGATTTCGTATAATTTCTGAACTTGGATTGTTTTGGGCTTTCTTTCCAAGACAGGTATTATTTCATTTTATAAAGATTTTCTCGAATCACTGTATCAGTTGCTTGGAGGTCATGTGTCTATAACAGGCAAGATATGAGTTGTTGACATTATTCGTAGCCCTTTCATTTTTTTTTCTATATGAAATCGTTGAATTTTTTATATTTGGGCTGACATTTTTTCTTGTTCTGTTTTTTCTTCTTTGGCCCTCCTTCTGTCTACTGTCAGCTATTGGGCATATATGTCAAACGAAGAAGGTACTTGGTCATGGGTCATATAAAGTTTATATCTGCTAGTTATGTTTAATTTTAATGCTTCAGAAAGCAAATTAGTTGATATTGATGTTGTTCATATGGATATATTGTAGTTTGTTATGTAAATTTCCCTGGTGCTTTTGTGGAATGTATATGACGTTTCTTCTCTTTTAGGACTGGGAAGGTGGAAGGTTATTTTCATTGCAAGAACAAATTGATCATAAGGTAACTGGCTGTTTTCTCCGTTGATTTGATTGAATTGTTGATGGGTTTAAAGTAGCTACGGTCCATGGAAAATTTAAATTTTGAAGTTGCTTTATAAGCTAGTGGACTTCAGTTTTCACGTCCAAGATAGGTATGCAATCGGAGATAGCTAAGCGGAACTTTGAAATTAACATCCCTTGCTCAAGATTACTGTTCTTTTCATTATTATCTGTTTTTTGCATACCCCTTTCTCTATCGTGTTTCAGTGGTTGATGCCAGAATTCTATACGGGGCTGGACAATATTCATCTTTCTTTTGCAGGTTGAATTTGTTAGACAGGAGTTACAGAATAAAGACATCCCATTACTACTGGTAATGATACTCTTATGATTGCAATTGATGCTATGTTTTCACAATAGTCATAAAAGAGTTGCACATTGCAAAATTTTCAATTGTATTATTTTGATATTATCTGAGTACACGTCAGATATTATCTGAAAATTTTCAATTTATAAAATTTGATTGTAGTTTAACACATACGCATACGTAATGGTTCGTTGTGGTGCTTGTATTATTGTTACTTGTTTGCAAAAAGAAAAAAAAAAAAGGTCCACGTTATTGTTTGTATTGTACATTATATGCATCTCAGATATACTTAATTGGAAATTGATATGAGATGATACAGGCCTTGAAAATAAGAATGAAGTTGTTACATTATTGTTTTTTATAACTGTTCTTTTCCACTTGTGGTAACTGACTTCTCGGTGTCTTTAAAGGTTGGACATTCTGTTGGATCGTATATCTCCATTGAATTGTTCCGCAGGTTCCAGGATCGGGTATTGCCATAAAATCTTTGTCCCTCCTGTGTCTTTACAACTTGCAGGAGATGGTTTATTGACAATTCTTCTTGACCCTATAGGCGGTGTATTGCATTGGTTTGCATCCATTTATGATGGTGAACAGAGAATCACGTCAGCAGTTTTTTATTGAAAAACTTGCAAGGTATGCATGTTATTTTTTCTGATAAAATGTATTATATATGGCTTCCTGAATCTATATTTCAGATTTTTTAGCAGTGATTTTGTTGCATATCACGATTTTCAAAATCTCCCATTTCACTTATATGTTGGTACCAATGGTGTGCTAGTGGCAACCATTTTCAATGAGTTCAAAGTTTCAAACTCTGCCTAAGGGAGCTTTTCAGTTTTTGCGAGTGTGAACCTAAAAATTTTTACTGCCTCAAACAGTTACACTCCACACCATGTGATGTGGCTTCATTGGATTGTTCAAAAATCTAGAATCCTCTAAGACCACAAACTGGTGTAATTTCGGAATCTTCAGATTTCTGTTGGTTGTGTTTTTGTGGGGAGTATTTGCATTTTCCAGTGGGTATGCACTTGGGTTGAACCAATTTGGGAACTTAGACTAATGGAGATATGTGCTTGGTCTATAACCAGTTAAGTGTGCCGAATACTACGTTTAACGTCTGGTTGAGTTTCATGGCTTCGTGACCTTGTTAACTATTTAGACCACCTTTTGGTGGCTGCTTTGTAGTTGGACCTTAATGGTTAGTAAAATTGGACCATGTAGAAGTCCTTCATACATTGTCCACAAACTGGATCTTAATCCTTTTAAGCATTCACTACTATGGCCGATATTACTTTGGTCCATGGCTGATTACAAACTAATAGATGAGGTCATTTTAAAGCTTTGGTATTCAAGAATGTCTTTGATGATGTTGTTTTCTTCTACCAAATATTATTGTTACTTTGTAAGGATTCACCTGAAATTTTTTTGTCGCAGGTCTCCACTGCTATCAACTTTGTTTTCTTCTTTTGCTGCGTTGTTGGGTACATTACCAATTCAAGCTTCAAGTTTTGTTGTGAAAAAAACTATTGGGAAGTCATGGTCCAGAACTGCTAGTGAGGCTGCTTGCTCTCATTTGCTAAAGGTGAAATGAAATCTAATTTACTTCTGTAGATGTGCTCTTATAGTATTGACTCGTAACTGCTATAGATTTTGGTTTGACTGTTTTTTCTGATATTTGGAATGTTGTCTTTGTTTCCTACAGTACCATAGCATGCGAAATGTGCTATATATGGCAATGACAGAGTTTGATAAGGTATACACTATAAAAAAAAATCACTTTAGTTTGGTAGACATGTTTCGGACATGTTGGATACCATAACACTTGCTGCCCATGTATCAAAAAAACTTTGCTTCACTGTACCATAGTCTTTTATTTATTTAAATTGAGAACTTTTGATTAAAAAGAACCATAAAAGAGAATTAATAAATATTTTAGAGTAAAAGACTTCCAACTTGCTGTCCAAACATATGATTGTACAAACCATGCTTAATTGTATAATACATTATTGTTATAAGCTTTGAGCTTGTTATAGTACAGTTGTCCAAGCATAAAGGGTTTAACTTAATTGTATAAGCTTTGAGACATTGCATTATTGTTATAGTACAGTAGTCCAAATATAGAAGCCTGGCCACAAAATGTAAATTTGATGACGTTCAGTCACAATATGTGAGTAAATGAAACTTGTCAATCAGAACATCGTTCTCCGTGTTTTCTTAATAGATTTTTGTACATGGTGGTCACTTCTCAGAATTATGCATTACAGAGCCTAATATCATTAATAATCAAATTTTCACATCTGATTGTACATGGACATTGTTTAGTTTTCAGAAACACCAGATTGGGCATTTATGAAGAAAGTGAGTCAGAAATTATCATTTTTGTTCTGTATGGATGACCATTGGGCTCCAATGCATGTGTACGAGGAGGTAAGATATAAACTACTTTCCATTTTGTGTATTAACTCTCTTGTATGATTGTAGGTTGTCCAAATACTATGCCTGAAGGGATTATAGTTATAATATTGTTTATAATGGATTAGTGAGTACATTTTATTGGCTCAGAATCCTTTTCTTTGTAATATAGTCAATAACATCAAAGCAGCTGAGGAACCATCATGGATTAGTCTAGTGGTAAATAACTGACTAAAAGAGAATGAGTTCAATCCATGCTAATGTTTAATATCCTATGAGTTTCCTTCTCAAATTTAGTGTCTGGCAAGTTATTCTATAAGATTAGTTGAGGTGTATGTAAGTTGGTCCCACAGTCGGCGATATATAAAAAAAGTTTCAAAATAGCAGGATTGAAATACTACATATTGTATTTTCAAGAAGAAAAAAGAGGGGCAAAAGAGCTCAAAATTTTGACACACTGCAATGGTGAATTCAAGTTATACTTTGTTAGTTTGTGCTCTCTTTTCTCCACAGATATCCAAGCAGGTCCCTGAAATTGATCTATCAGTTGAAAGGGAGGGCTATTCACATGCCTTTTGCTGCTCTGAAGCTGCCTCAATGTATATTGCTCAATATGTGGCTAGCTTGGTCAAGAAACATCTCTCAGATTAAAGGTCAGATTCATTTCTTCTTACTCCATTAGAGATTTCCTTTGCGTTTTCTTCTATGGGACTTCCTTAATCTTTTATACCATTTTGCATGTTTTTCAACAGTTTCTCCCCGTGCTTTTATCCTTATATAAAGAACTCGTGTTGTTGACAAAATAGATGAAAAGGAATAATAAAAGCACCTTCTGATGTCGAGCATAACGAGTCGAGGCACCATATGTTGTAGTATTGAGAATCAAATTATGGGTTAGTATTTTTTTGAACAAGAAATAAATGTCATCGAATCAAATTATTAGTTGTTTTTAAGAAGGTGATTCGTCTAGTTTATAAGGCTTAACGAAACAGATCTTATTTATGTAACTTTAGCCTCCACTGAAGGAG

mRNA sequence

ATGAATTTTTAAAAAACCAATTATAAAAAACGAAATGGTTTATCATTGGGGGAATCTTGATATTTATTAAAAATCAACAGATGGATTCATTATGATCCATACTCGAAAGAGAACATGAATTGCGGGGATTTCGAGAGCCGTAGAAGAATCCATGTTCTTACCGCTTCCTTCACTCACCGTCCGACCTACCCTTCTCATATCATATTCATATTCCAGGTTGAAGAATTTCTCTAGGTGTTCGTTTCTCCATATGGGTCATCAAGTTCCGCAATCTTTTAGTAAAAGGCGTGTGGAGTTTCGTTTATGCAATGTATCGGGTTTTACCAATGAATTACTGGAGATTCACTCGGATGATCCATCATTGCACGTATTGTTTATTCCTGGAAATCCAGGTATTATTTCATTTTATAAAGATTTTCTCGAATCACTGTATCAGTTGCTTGGAGGTCATGTGTCTATAACAGCTATTGGGCATATATGTCAAACGAAGAAGGACTGGGAAGGTGGAAGGTTATTTTCATTGCAAGAACAAATTGATCATAAGGTTGAATTTGTTAGACAGGAGTTACAGAATAAAGACATCCCATTACTACTGGTTGGACATTCTGTTGGATCGTATATCTCCATTGAATTGTTCCGCAGGTTCCAGGATCGGGCGGTGTATTGCATTGGTTTGCATCCATTTATGATGGTGAACAGAGAATCACGTCAGCAGTTTTTTATTGAAAAACTTGCAAGGTCTCCACTGCTATCAACTTTGTTTTCTTCTTTTGCTGCGTTGTTGGGTACATTACCAATTCAAGCTTCAAGTTTTGTTGTGAAAAAAACTATTGGGAAGTCATGGTCCAGAACTGCTAGTGAGGCTGCTTGCTCTCATTTGCTAAAGTACCATAGCATGCGAAATGTGCTATATATGGCAATGACAGAGTTTGATAAGTTTTCAGAAACACCAGATTGGGCATTTATGAAGAAAGTGAGTCAGAAATTATCATTTTTGTTCTGTATGGATGACCATTGGGCTCCAATGCATGTGTACGAGGAGATATCCAAGCAGGTCCCTGAAATTGATCTATCAGTTGAAAGGGAGGGCTATTCACATGCCTTTTGCTGCTCTGAAGCTGCCTCAATGTATATTGCTCAATATGTGGCTAGCTTGGTCAAGAAACATCTCTCAGATTAAAGTTTCTCCCCGTGCTTTTATCCTTATATAAAGAACTCGTGTTGTTGACAAAATAGATGAAAAGGAATAATAAAAGCACCTTCTGATGTCGAGCATAACGAGTCGAGGCACCATATGTTGTAGTATTGAGAATCAAATTATGGGTTAGTATTTTTTTGAACAAGAAATAAATGTCATCGAATCAAATTATTAGTTGTTTTTAAGAAGGTGATTCGTCTAGTTTATAAGGCTTAACGAAACAGATCTTATTTATGTAACTTTAGCCTCCACTGAAGGAG

Coding sequence (CDS)

ATGTTCTTACCGCTTCCTTCACTCACCGTCCGACCTACCCTTCTCATATCATATTCATATTCCAGGTTGAAGAATTTCTCTAGGTGTTCGTTTCTCCATATGGGTCATCAAGTTCCGCAATCTTTTAGTAAAAGGCGTGTGGAGTTTCGTTTATGCAATGTATCGGGTTTTACCAATGAATTACTGGAGATTCACTCGGATGATCCATCATTGCACGTATTGTTTATTCCTGGAAATCCAGGTATTATTTCATTTTATAAAGATTTTCTCGAATCACTGTATCAGTTGCTTGGAGGTCATGTGTCTATAACAGCTATTGGGCATATATGTCAAACGAAGAAGGACTGGGAAGGTGGAAGGTTATTTTCATTGCAAGAACAAATTGATCATAAGGTTGAATTTGTTAGACAGGAGTTACAGAATAAAGACATCCCATTACTACTGGTTGGACATTCTGTTGGATCGTATATCTCCATTGAATTGTTCCGCAGGTTCCAGGATCGGGCGGTGTATTGCATTGGTTTGCATCCATTTATGATGGTGAACAGAGAATCACGTCAGCAGTTTTTTATTGAAAAACTTGCAAGGTCTCCACTGCTATCAACTTTGTTTTCTTCTTTTGCTGCGTTGTTGGGTACATTACCAATTCAAGCTTCAAGTTTTGTTGTGAAAAAAACTATTGGGAAGTCATGGTCCAGAACTGCTAGTGAGGCTGCTTGCTCTCATTTGCTAAAGTACCATAGCATGCGAAATGTGCTATATATGGCAATGACAGAGTTTGATAAGTTTTCAGAAACACCAGATTGGGCATTTATGAAGAAAGTGAGTCAGAAATTATCATTTTTGTTCTGTATGGATGACCATTGGGCTCCAATGCATGTGTACGAGGAGATATCCAAGCAGGTCCCTGAAATTGATCTATCAGTTGAAAGGGAGGGCTATTCACATGCCTTTTGCTGCTCTGAAGCTGCCTCAATGTATATTGCTCAATATGTGGCTAGCTTGGTCAAGAAACATCTCTCAGATTAA

Protein sequence

MFLPLPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD*

Homology

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BLAST of CSPI06G25760 vs. ExPASy Swiss-Prot
Match: Q5HZX7 (Lipid droplet-associated hydrolase OS=Rattus norvegicus OX=10116 GN=Ldah PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.8e-25
Identity = 87/312 (27.88%), Postives = 142/312 (45.51%), Query Frame = 0


Query: 47  VEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAI 106
           VE ++     +TN   +     P   +  IPGNPG+  FY  F ++LY L+ GH  +  I
Sbjct: 21  VETQIIKCGPWTNLFDKQGVSKPKHLIFVIPGNPGLSPFYVPFAKALYSLVKGHFPVWII 80

Query: 107 GH--ICQTKKD------------WEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHS 166
            H   C T KD             E   ++ L  QI+HK+ F+R  +  KD+ L+ +GHS
Sbjct: 81  SHAGFCLTPKDKKILTAPQEPNAQEIEDIYGLNGQIEHKIAFLRAHVP-KDVKLIFIGHS 140

Query: 167 VGSYISIELFRRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLARSPLLS----TLFSSFA 226
           VGSYI++ +  R  +  V    +H F++     R          +P L      L+++  
Sbjct: 141 VGSYIALHVMNRAPELPV----VHTFLLFPTIERMSESPNGKFATPFLCRFRYMLYAASY 200

Query: 227 ALLGTLPIQASSFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPD 286
            +    P    SF+V+K + K   +  SE   + LL+   + N  Y+   E     +  D
Sbjct: 201 LIFKPCPEMIKSFIVQKVLEKMSFK--SELRLTDLLQPFCLANAAYLGGQEMIHVVKRDD 260

Query: 287 WAFMKKVSQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASMYI 341
              +K++  KL+F +   D W P++ YE++ +  PE +L +  +G  HAF    +  M  
Sbjct: 261 -GIIKELLPKLTFYYGKTDGWCPVNYYEDMKRDFPEANLHLCEKGIPHAFVLGFSQEM-- 320

[Close]

BLAST of CSPI06G25760 vs. ExPASy Swiss-Prot
Match: Q54LL8 (Lipid droplet-associated hydrolase OS=Dictyostelium discoideum OX=44689 GN=DDB_G0286581 PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 3.1e-22
Identity = 87/308 (28.25%), Postives = 145/308 (47.08%), Query Frame = 0


Query: 60  ELLEIHSDDPS-LHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEG 119
           E++   S  PS + ++ I GNPGI SFY++F++ L         I  +GHI    K    
Sbjct: 21  EIIYTKSQTPSNIKIIVIAGNPGIESFYQEFVKVLNLSFNSKYDIYGVGHIGHCGK--IE 80

Query: 120 GRLFSLQEQIDHKVEFVRQELQN-------KDIPLLLVGHSVGSYISIELFRRFQDRAVY 179
            + FS++EQI HK  F+   L+N       KDI  +L+GHSVGSYIS+++  RF ++  +
Sbjct: 81  NKTFSVEEQIKHKELFLEYLLKNKYGDKDRKDIKFILIGHSVGSYISLKVVSRFSEKFEF 140

Query: 180 CI-------------GLHPF--MMVNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPI 239
                          GL PF  M+V RES +                 +  +  L  +P 
Sbjct: 141 LSVVNLFPTFKNLYDGLSPFIKMVVMRESTR-----------------NGLSTFLHYIPS 200

Query: 240 QASSFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPD---WAFMK 299
              S V+K  +    SR A ++     + Y+S  N+LYMA TE +   E  D     F  
Sbjct: 201 IVVSNVLKWILPSDESRIAVQSK----INYYSALNILYMAYTETEDIKEIDDECHSVFNS 260

Query: 300 KVSQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCC--SEAASMYIAQY 340
           +++Q L F++   D + P   Y+E+ +  P  ++        HAF    S+  ++ ++++
Sbjct: 261 RLNQ-LLFIYGQTDSYTPKSFYDEMKQLYPAGNIEYSSSYVPHAFVLHHSQEVALRVSEW 304

[Close]

BLAST of CSPI06G25760 vs. ExPASy Swiss-Prot
Match: Q8BVA5 (Lipid droplet-associated hydrolase OS=Mus musculus OX=10090 GN=Ldah PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 7.7e-21
Identity = 77/307 (25.08%), Postives = 138/307 (44.95%), Query Frame = 0


Query: 47  VEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAI 106
           VE ++     +TN   +     P   +  IPGNPG  +FY  F ++LY L+     +  I
Sbjct: 21  VETKIIKCGPWTNLFEKQDVSKPKQLIFIIPGNPGYSAFYVPFAKALYTLMKSRFPVWII 80

Query: 107 GH--ICQTKKD-------------WEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGH 166
            H     T KD              +   ++ L  QI+HK+ F+R  +  KD+ L+L+GH
Sbjct: 81  SHAGFSVTPKDKKVLAAPQEESNAQKIEDVYGLNGQIEHKIAFLRAHVP-KDVKLILIGH 140

Query: 167 SVGSYISIELFRRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLARSPLLS----TLFSSF 226
           SVG+Y+++ + +R  +  V     H F++     R          +P L      L+++ 
Sbjct: 141 SVGTYMTLHVMKRVLELPV----AHAFLLFPTIERMSESPNGKFATPFLCQFRYLLYATS 200

Query: 227 AALLGTLPIQASSFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETP 286
             L    P    SF+++K +G+       E   + +L+   + N  Y+   E  +  +  
Sbjct: 201 YLLFKPCPEVIKSFIIQKLMGQ--MNIKLELPLTDILQPFCLANAAYLGSQEMVQIVKRD 260

Query: 287 DWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCC--SEAAS 333
           D   +K+   KL F +   D W P+  YE++ K  PE ++ +  +G  HAF    S+  +
Sbjct: 261 D-DIIKEFLPKLKFYYGKTDGWCPVKYYEDMKKDFPEGNIYLCEKGIPHAFVLDFSQEMA 319

[Close]

BLAST of CSPI06G25760 vs. ExPASy Swiss-Prot
Match: Q5F477 (Lipid droplet-associated hydrolase OS=Gallus gallus OX=9031 GN=LDAH PE=2 SV=2)

HSP 1 Score: 102.4 bits (254), Expect = 1.0e-20
Identity = 76/275 (27.64%), Postives = 127/275 (46.18%), Query Frame = 0


Query: 69  PSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTK-------------KD 128
           P L  + IPGNPG+  +Y+ F+++LY  L     +  + H    K             K+
Sbjct: 43  PRLLFMIIPGNPGLAGYYRTFIKALYCGLNQQYPVWVVSHAGHCKPPSGMEMIEDTDIKE 102

Query: 129 WEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGL 188
            E   +F L  Q++HK+ F+++ + +KDI L+L+ HS+G YI++E+ +R  +  V    L
Sbjct: 103 LED--VFGLNGQVEHKLNFLKKNV-SKDIKLVLIAHSIGCYITLEMMKRASELQV----L 162

Query: 189 HPFMMVNRESRQQFFIEKLARSPLLSTLFSSF---AALLGTLPIQASSFVVKKTI-GKSW 248
              ++     R     +    +PLL  L  +      LL  LP    + +V+  + G   
Sbjct: 163 RSVLLFPTIERMAQSPQGKLMTPLLCKLRYALYMPVYLLSFLPEGVKASLVRFALRGMKT 222

Query: 249 SRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAP 308
              +S     +L     + N+LYMA  E  K  E  D   +K+  +KL F +   D W P
Sbjct: 223 CDESSITTSVNLFSVDCIANILYMASQEMMKVVER-DSTTIKQNLKKLIFYYGTGDSWCP 282

Query: 309 MHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASM 327
            + Y+EI    P+ D+ +  +G  HAF    +  M
Sbjct: 283 QNYYDEIKMDFPDGDIRLCEKGLRHAFVLDASKEM 309

[Close]

BLAST of CSPI06G25760 vs. ExPASy Swiss-Prot
Match: Q9H6V9 (Lipid droplet-associated hydrolase OS=Homo sapiens OX=9606 GN=LDAH PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.2e-19
Identity = 79/291 (27.15%), Postives = 130/291 (44.67%), Query Frame = 0


Query: 69  PSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGH-----------ICQTKKD-- 128
           P L +  IPGNPG  +FY  F ++LY L      +  I H           I  T +D  
Sbjct: 43  PKLLIFIIPGNPGFSAFYVPFAKALYSLTNRRFPVWTISHAGHALAPKDKKILTTSEDSN 102

Query: 129 -WEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIG 188
             E   ++ L  QI+HK+ F+R  +  KD+ L+L+GHS+GSY ++++ +R  +  V    
Sbjct: 103 AQEIKDIYGLNGQIEHKLAFLRTHVP-KDMKLVLIGHSIGSYFTLQMLKRVPELPV---- 162

Query: 189 LHPFMMVNRESRQQFFIEKLARSPLLS----TLFSSFAALLGTLPIQASSFVVKKTIGKS 248
           +  F++     R          +PLL      L+ +   LL   P    S ++++  G  
Sbjct: 163 IRAFLLFPTIERMSESPNGRIATPLLCWFRYVLYVTGYLLLKPCPETIKSLLIRR--GLQ 222

Query: 249 WSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWA 308
                +E +  ++L+   + N  Y+   E  +  +  D   +K+   KL+F +   D W 
Sbjct: 223 VMNLENEFSPLNILEPFCLANAAYLGGQEMMEVVKRDD-ETIKEHLCKLTFYYGTIDPWC 282

Query: 309 PMHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLS 342
           P   YE+I K  PE D+ +  +   HAF       M  A  +A  +K  LS
Sbjct: 283 PKEYYEDIKKDFPEGDIRLCEKNIPHAFITHFNQEM--ADMIADSLKDDLS 323

[Close]

BLAST of CSPI06G25760 vs. ExPASy TrEMBL
Match: A0A0A0KFF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454400 PE=3 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 6.5e-196
Identity = 341/342 (99.71%), Postives = 342/342 (100.00%), Query Frame = 0


Query: 1   MFLPLPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNE 60
           MFLPLPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNE
Sbjct: 1   MFLPLPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNE 60

Query: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGR 120
           LLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGR
Sbjct: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGR 120

Query: 121 LFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180
           LFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM
Sbjct: 121 LFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180

Query: 181 VNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAAC 240
           VNRESRQQFFIEKL+RSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAAC
Sbjct: 181 VNRESRQQFFIEKLSRSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAAC 240

Query: 241 SHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISK 300
           SHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISK
Sbjct: 241 SHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISK 300

Query: 301 QVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD 343
           QVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD
Sbjct: 301 QVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD 342

[Close]

BLAST of CSPI06G25760 vs. ExPASy TrEMBL
Match: A0A5A7SKD4 (Lipid droplet-associated hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001690 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.0e-188
Identity = 328/342 (95.91%), Postives = 335/342 (97.95%), Query Frame = 0


Query: 1   MFLPLPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNE 60
           MFL LPSLTVRPT+LISYSYSRLKNFSRCSFLHMGHQV QSFSKRRVEFRLCNVSGFTNE
Sbjct: 1   MFLQLPSLTVRPTVLISYSYSRLKNFSRCSFLHMGHQVLQSFSKRRVEFRLCNVSGFTNE 60

Query: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGR 120
           LLEIHSDDPSLHVLFIPGNPGIISFYKDF+ESLYQLLGGHVSITAIGHICQTKKDWEGGR
Sbjct: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFVESLYQLLGGHVSITAIGHICQTKKDWEGGR 120

Query: 121 LFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180
           LFSLQEQIDHKVEFVRQELQNKDIPL+LVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM
Sbjct: 121 LFSLQEQIDHKVEFVRQELQNKDIPLILVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180

Query: 181 VNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAAC 240
           VN+ESRQQFFIEKLARSPLLST FSSF ALLG LPIQASSFVV+KTIGKSWSRTA EAAC
Sbjct: 181 VNKESRQQFFIEKLARSPLLSTFFSSFVALLGILPIQASSFVVRKTIGKSWSRTACEAAC 240

Query: 241 SHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISK 300
           SHLLKYHSMRNVLYMAMTEF+KFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEI K
Sbjct: 241 SHLLKYHSMRNVLYMAMTEFEKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEIFK 300

Query: 301 QVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD 343
           QVPEIDLSVEREGYSHAFCCSEAAS+YIAQYVASLVKKHLSD
Sbjct: 301 QVPEIDLSVEREGYSHAFCCSEAASIYIAQYVASLVKKHLSD 342

[Close]

BLAST of CSPI06G25760 vs. ExPASy TrEMBL
Match: A0A1S3CHU9 (LOW QUALITY PROTEIN: lipid droplet-associated hydrolase OS=Cucumis melo OX=3656 GN=LOC103500925 PE=3 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 5.0e-188
Identity = 327/342 (95.61%), Postives = 334/342 (97.66%), Query Frame = 0


Query: 1   MFLPLPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNE 60
           MFL LPSLTVRPT+LISYSYSRLKNFSRCSFLHMGHQV QSFSKRRVEFRLCNVSGFTNE
Sbjct: 1   MFLQLPSLTVRPTVLISYSYSRLKNFSRCSFLHMGHQVLQSFSKRRVEFRLCNVSGFTNE 60

Query: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGR 120
           LLEIHSDDPSLHVLFIPGNPGIISFYKDF+ESLYQLLGGHVSITAIGHICQTKKDWEGGR
Sbjct: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFVESLYQLLGGHVSITAIGHICQTKKDWEGGR 120

Query: 121 LFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180
           LFSLQEQIDHKVEFVRQELQNKDIPL+LVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM
Sbjct: 121 LFSLQEQIDHKVEFVRQELQNKDIPLILVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180

Query: 181 VNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAAC 240
           VN+ESRQQFFIEKLARSPLLS  FSSF ALLG LPIQASSFVV+KTIGKSWSRTA EAAC
Sbjct: 181 VNKESRQQFFIEKLARSPLLSXFFSSFVALLGILPIQASSFVVRKTIGKSWSRTACEAAC 240

Query: 241 SHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISK 300
           SHLLKYHSMRNVLYMAMTEF+KFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEI K
Sbjct: 241 SHLLKYHSMRNVLYMAMTEFEKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEIFK 300

Query: 301 QVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD 343
           QVPEIDLSVEREGYSHAFCCSEAAS+YIAQYVASLVKKHLSD
Sbjct: 301 QVPEIDLSVEREGYSHAFCCSEAASIYIAQYVASLVKKHLSD 342

[Close]

BLAST of CSPI06G25760 vs. ExPASy TrEMBL
Match: A0A6J1BTR5 (lipid droplet-associated hydrolase OS=Momordica charantia OX=3673 GN=LOC111005683 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.9e-156
Identity = 272/343 (79.30%), Postives = 302/343 (88.05%), Query Frame = 0


Query: 1   MFLP-LPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTN 60
           MFL  LPSLTV  T    +  S+LK FSRC+F+HMG +V  SFS+R  EFR CNVSGFTN
Sbjct: 14  MFLRLLPSLTVGSTNFSFFFNSKLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTN 73

Query: 61  ELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGG 120
           ELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGG VSITAIGH+ QTKKDWEGG
Sbjct: 74  ELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGRVSITAIGHVSQTKKDWEGG 133

Query: 121 RLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFM 180
           RLFSLQEQIDHK+EFVRQEL N DIPLLLVGHS+GSYISIELFRR QD+ VYCIGLHPF+
Sbjct: 134 RLFSLQEQIDHKIEFVRQELPNNDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFL 193

Query: 181 MVNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAA 240
           M+N++SRQQ  I K A SPLLSTLFS+  AL+G LP +A SFVV+KTI KSWS TA+EAA
Sbjct: 194 MINKQSRQQLLIGKFAGSPLLSTLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAA 253

Query: 241 CSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEIS 300
           CSHLLKYH+MRNVL+MAMTEF + SE PDWAFMKK SQKLSFLFC+DDHWAPMH+YEEIS
Sbjct: 254 CSHLLKYHTMRNVLFMAMTEFKELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEIS 313

Query: 301 KQVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD 343
           KQVPEIDLSVEREG++HAFCCSEAAS+YIAQ+VA+L+K HL D
Sbjct: 314 KQVPEIDLSVEREGHTHAFCCSEAASLYIAQHVANLIKNHLPD 356

[Close]

BLAST of CSPI06G25760 vs. ExPASy TrEMBL
Match: A0A6J1I921 (lipid droplet-associated hydrolase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471128 PE=3 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 3.6e-154
Identity = 271/351 (77.21%), Postives = 306/351 (87.18%), Query Frame = 0


Query: 1   MFLPLPSLTVRPTLLISYSYS---------RLKNFSRCSFLHMGHQVPQSFSKRRVEFRL 60
           MFL LPSLTVR   L  +  S         RLK FSR +F+HMG +V   FS+ R +FRL
Sbjct: 1   MFLNLPSLTVRSINLPFFFSSVLWLPNCSFRLKKFSRSAFVHMGQEVLNPFSRWRAQFRL 60

Query: 61  CNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQ 120
           CNVSG+TNELLEIH+DDPSLHVLFIPGNPGI+SFYK F+ESLYQLLGG VSITAIGH+ Q
Sbjct: 61  CNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKYFVESLYQLLGGRVSITAIGHLSQ 120

Query: 121 TKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVY 180
           T KDWEGGRLFSLQEQIDHK+EFVRQELQ+KDIPLLLVGHS+GSYISIEL+R+FQDR VY
Sbjct: 121 TTKDWEGGRLFSLQEQIDHKIEFVRQELQSKDIPLLLVGHSIGSYISIELYRKFQDRVVY 180

Query: 181 CIGLHPFMMVNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSW 240
           CIGLHPFMMVNR+SRQQF I KLA S LLSTLFSSF ALLG LP + SSFVV KTIGKSW
Sbjct: 181 CIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGVLPSRVSSFVVGKTIGKSW 240

Query: 241 SRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAP 300
           SRTASEAAC++LLKYH MRN+L+MA+TEF+K SETPDW FMKK S+KLSFLFCMDDHWAP
Sbjct: 241 SRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWGFMKKSSRKLSFLFCMDDHWAP 300

Query: 301 MHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD 343
           MHV+EEISKQ+PE+D+SVEREG++HAFCCSEA S+YIAQ+VASL+K H+SD
Sbjct: 301 MHVFEEISKQIPEMDVSVEREGHTHAFCCSEAGSLYIAQHVASLIKNHISD 351

[Close]

BLAST of CSPI06G25760 vs. NCBI nr
Match: XP_004143334.1 (lipid droplet-associated hydrolase isoform X1 [Cucumis sativus] >KGN48293.1 hypothetical protein Csa_004459 [Cucumis sativus])

HSP 1 Score: 693.0 bits (1787), Expect = 1.3e-195
Identity = 341/342 (99.71%), Postives = 342/342 (100.00%), Query Frame = 0


Query: 1   MFLPLPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNE 60
           MFLPLPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNE
Sbjct: 1   MFLPLPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNE 60

Query: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGR 120
           LLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGR
Sbjct: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGR 120

Query: 121 LFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180
           LFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM
Sbjct: 121 LFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180

Query: 181 VNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAAC 240
           VNRESRQQFFIEKL+RSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAAC
Sbjct: 181 VNRESRQQFFIEKLSRSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAAC 240

Query: 241 SHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISK 300
           SHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISK
Sbjct: 241 SHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISK 300

Query: 301 QVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD 343
           QVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD
Sbjct: 301 QVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD 342

[Close]

BLAST of CSPI06G25760 vs. NCBI nr
Match: KAA0025227.1 (lipid droplet-associated hydrolase [Cucumis melo var. makuwa] >TYK07439.1 lipid droplet-associated hydrolase [Cucumis melo var. makuwa])

HSP 1 Score: 669.1 bits (1725), Expect = 2.1e-188
Identity = 328/342 (95.91%), Postives = 335/342 (97.95%), Query Frame = 0


Query: 1   MFLPLPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNE 60
           MFL LPSLTVRPT+LISYSYSRLKNFSRCSFLHMGHQV QSFSKRRVEFRLCNVSGFTNE
Sbjct: 1   MFLQLPSLTVRPTVLISYSYSRLKNFSRCSFLHMGHQVLQSFSKRRVEFRLCNVSGFTNE 60

Query: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGR 120
           LLEIHSDDPSLHVLFIPGNPGIISFYKDF+ESLYQLLGGHVSITAIGHICQTKKDWEGGR
Sbjct: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFVESLYQLLGGHVSITAIGHICQTKKDWEGGR 120

Query: 121 LFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180
           LFSLQEQIDHKVEFVRQELQNKDIPL+LVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM
Sbjct: 121 LFSLQEQIDHKVEFVRQELQNKDIPLILVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180

Query: 181 VNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAAC 240
           VN+ESRQQFFIEKLARSPLLST FSSF ALLG LPIQASSFVV+KTIGKSWSRTA EAAC
Sbjct: 181 VNKESRQQFFIEKLARSPLLSTFFSSFVALLGILPIQASSFVVRKTIGKSWSRTACEAAC 240

Query: 241 SHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISK 300
           SHLLKYHSMRNVLYMAMTEF+KFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEI K
Sbjct: 241 SHLLKYHSMRNVLYMAMTEFEKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEIFK 300

Query: 301 QVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD 343
           QVPEIDLSVEREGYSHAFCCSEAAS+YIAQYVASLVKKHLSD
Sbjct: 301 QVPEIDLSVEREGYSHAFCCSEAASIYIAQYVASLVKKHLSD 342

[Close]

BLAST of CSPI06G25760 vs. NCBI nr
Match: XP_008462607.1 (PREDICTED: LOW QUALITY PROTEIN: lipid droplet-associated hydrolase [Cucumis melo])

HSP 1 Score: 666.8 bits (1719), Expect = 1.0e-187
Identity = 327/342 (95.61%), Postives = 334/342 (97.66%), Query Frame = 0


Query: 1   MFLPLPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNE 60
           MFL LPSLTVRPT+LISYSYSRLKNFSRCSFLHMGHQV QSFSKRRVEFRLCNVSGFTNE
Sbjct: 1   MFLQLPSLTVRPTVLISYSYSRLKNFSRCSFLHMGHQVLQSFSKRRVEFRLCNVSGFTNE 60

Query: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGR 120
           LLEIHSDDPSLHVLFIPGNPGIISFYKDF+ESLYQLLGGHVSITAIGHICQTKKDWEGGR
Sbjct: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFVESLYQLLGGHVSITAIGHICQTKKDWEGGR 120

Query: 121 LFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180
           LFSLQEQIDHKVEFVRQELQNKDIPL+LVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM
Sbjct: 121 LFSLQEQIDHKVEFVRQELQNKDIPLILVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180

Query: 181 VNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAAC 240
           VN+ESRQQFFIEKLARSPLLS  FSSF ALLG LPIQASSFVV+KTIGKSWSRTA EAAC
Sbjct: 181 VNKESRQQFFIEKLARSPLLSXFFSSFVALLGILPIQASSFVVRKTIGKSWSRTACEAAC 240

Query: 241 SHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISK 300
           SHLLKYHSMRNVLYMAMTEF+KFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEI K
Sbjct: 241 SHLLKYHSMRNVLYMAMTEFEKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEIFK 300

Query: 301 QVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD 343
           QVPEIDLSVEREGYSHAFCCSEAAS+YIAQYVASLVKKHLSD
Sbjct: 301 QVPEIDLSVEREGYSHAFCCSEAASIYIAQYVASLVKKHLSD 342

[Close]

BLAST of CSPI06G25760 vs. NCBI nr
Match: XP_038881163.1 (lipid droplet-associated hydrolase [Benincasa hispida])

HSP 1 Score: 632.5 bits (1630), Expect = 2.1e-177
Identity = 311/342 (90.94%), Postives = 323/342 (94.44%), Query Frame = 0


Query: 1   MFLPLPSLTVRPTLLISYSYSRLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNE 60
           MFL LPSLTVR T    +SYSRLKNF+R SFLHMGHQV QSFSK RVE RLCNVSG+TNE
Sbjct: 1   MFLQLPSLTVRSTRFTFFSYSRLKNFTRGSFLHMGHQVLQSFSKSRVELRLCNVSGYTNE 60

Query: 61  LLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGR 120
           LLEIHSD PSLHVLFIPGNPGIISFYKDF+ESLYQLLGG VSITAIGHICQTKKDWEGGR
Sbjct: 61  LLEIHSDHPSLHVLFIPGNPGIISFYKDFVESLYQLLGGRVSITAIGHICQTKKDWEGGR 120

Query: 121 LFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMM 180
           LFSLQEQIDHK+EFVRQELQNKDIPLLLVGHSVGSYISIEL+RRFQDR VYCIGLHPFMM
Sbjct: 121 LFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSVGSYISIELYRRFQDRVVYCIGLHPFMM 180

Query: 181 VNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAAC 240
           VNR+SRQQFFIEKLARS LLSTLFSSF ALLG LPI+ASSFVV+KT GKSWSRTASEAAC
Sbjct: 181 VNRQSRQQFFIEKLARSSLLSTLFSSFVALLGVLPIRASSFVVRKTFGKSWSRTASEAAC 240

Query: 241 SHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISK 300
           SHLLKYHSMRNVLYMAM EF+KFSETPDWAFMKK SQKLSFLFCMDDHWAPMHVYEEISK
Sbjct: 241 SHLLKYHSMRNVLYMAMMEFEKFSETPDWAFMKKASQKLSFLFCMDDHWAPMHVYEEISK 300

Query: 301 QVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLSD 343
           QVPEIDLSVEREG+SHAFCCSEAAS+YIAQYVASLVKKHLSD
Sbjct: 301 QVPEIDLSVEREGHSHAFCCSEAASLYIAQYVASLVKKHLSD 342

[Close]

BLAST of CSPI06G25760 vs. NCBI nr
Match: XP_011657723.1 (lipid droplet-associated hydrolase isoform X2 [Cucumis sativus])

HSP 1 Score: 629.4 bits (1622), Expect = 1.8e-176
Identity = 308/309 (99.68%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 34  MGHQVPQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESL 93
           MGHQVPQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESL
Sbjct: 1   MGHQVPQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESL 60

Query: 94  YQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSV 153
           YQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSV
Sbjct: 61  YQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSV 120

Query: 154 GSYISIELFRRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLARSPLLSTLFSSFAALLGT 213
           GSYISIELFRRFQDRAVYCIGLHPFMMVNRESRQQFFIEKL+RSPLLSTLFSSFAALLGT
Sbjct: 121 GSYISIELFRRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLSRSPLLSTLFSSFAALLGT 180

Query: 214 LPIQASSFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMK 273
           LPIQASSFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMK
Sbjct: 181 LPIQASSFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMK 240

Query: 274 KVSQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASMYIAQYVA 333
           KVSQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASMYIAQYVA
Sbjct: 241 KVSQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASMYIAQYVA 300

Query: 334 SLVKKHLSD 343
           SLVKKHLSD
Sbjct: 301 SLVKKHLSD 309

[Close]

BLAST of CSPI06G25760 vs. TAIR 10
Match: AT3G11620.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 374.4 bits (960), Expect = 9.8e-104
Identity = 168/299 (56.19%), Postives = 229/299 (76.59%), Query Frame = 0


Query: 43  SKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVS 102
           +KR V+ RLC VSG   E++EI +++P+ HVLFIPGNPG++SFYKDFLESLY+ LGG+ S
Sbjct: 11  TKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYEFLGGNAS 70

Query: 103 ITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELF 162
           + AIG I  T KDWE GRLFS QEQIDHK++F+RQEL++  +P++LVGHS+GSYIS+EL 
Sbjct: 71  VIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGSYISLELL 130

Query: 163 RRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFV 222
           ++F D+ VYCIGL+PF+ +N++S +Q  I KLA S +LS   S   A L  LP+ A+  +
Sbjct: 131 KKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLPMSAARLL 190

Query: 223 VKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFL 282
           V K+IG SWS TA +A C+HL +YH+MRNVL+MA +EF + +  PDW FM++   KL+FL
Sbjct: 191 VSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFRELAAEPDWDFMRENQSKLAFL 250

Query: 283 FCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLS 342
           F +DDHW P+ ++EEISKQ P   LS+EREG++H FCC+ A S ++AQ+VA+L+K   S
Sbjct: 251 FGIDDHWGPLQLFEEISKQAPHTSLSIEREGHTHGFCCTVAGSAWVAQHVATLIKNRFS 309

[Close]

BLAST of CSPI06G25760 vs. TAIR 10
Match: AT3G11620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 374.4 bits (960), Expect = 9.8e-104
Identity = 168/299 (56.19%), Postives = 229/299 (76.59%), Query Frame = 0


Query: 43  SKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVS 102
           +KR V+ RLC VSG   E++EI +++P+ HVLFIPGNPG++SFYKDFLESLY+ LGG+ S
Sbjct: 11  TKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYEFLGGNAS 70

Query: 103 ITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELF 162
           + AIG I  T KDWE GRLFS QEQIDHK++F+RQEL++  +P++LVGHS+GSYIS+EL 
Sbjct: 71  VIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGSYISLELL 130

Query: 163 RRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFV 222
           ++F D+ VYCIGL+PF+ +N++S +Q  I KLA S +LS   S   A L  LP+ A+  +
Sbjct: 131 KKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLPMSAARLL 190

Query: 223 VKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMKKVSQKLSFL 282
           V K+IG SWS TA +A C+HL +YH+MRNVL+MA +EF + +  PDW FM++   KL+FL
Sbjct: 191 VSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFRELAAEPDWDFMRENQSKLAFL 250

Query: 283 FCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASMYIAQYVASLVKKHLS 342
           F +DDHW P+ ++EEISKQ P   LS+EREG++H FCC+ A S ++AQ+VA+L+K   S
Sbjct: 251 FGIDDHWGPLQLFEEISKQAPHTSLSIEREGHTHGFCCTVAGSAWVAQHVATLIKNRFS 309

[Close]

BLAST of CSPI06G25760 vs. TAIR 10
Match: AT3G11620.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 278.9 bits (712), Expect = 5.6e-75
Identity = 127/218 (58.26%), Postives = 170/218 (77.98%), Query Frame = 0


Query: 43  SKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVS 102
           +KR V+ RLC VSG   E++EI +++P+ HVLFIPGNPG++SFYKDFLESLY+ LGG+ S
Sbjct: 11  TKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYEFLGGNAS 70

Query: 103 ITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELF 162
           + AIG I  T KDWE GRLFS QEQIDHK++F+RQEL++  +P++LVGHS+GSYIS+EL 
Sbjct: 71  VIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGSYISLELL 130

Query: 163 RRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFV 222
           ++F D+ VYCIGL+PF+ +N++S +Q  I KLA S +LS   S   A L  LP+ A+  +
Sbjct: 131 KKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLPMSAARLL 190

Query: 223 VKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEF 261
           V K+IG SWS TA +A C+HL +YH+MRNVL+MA +EF
Sbjct: 191 VSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEF 228

[Close]

BLAST of CSPI06G25760 vs. TAIR 10
Match: AT3G11620.4 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 278.9 bits (712), Expect = 5.6e-75
Identity = 127/218 (58.26%), Postives = 170/218 (77.98%), Query Frame = 0


Query: 43  SKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGHVS 102
           +KR V+ RLC VSG   E++EI +++P+ HVLFIPGNPG++SFYKDFLESLY+ LGG+ S
Sbjct: 11  TKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYEFLGGNAS 70

Query: 103 ITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSVGSYISIELF 162
           + AIG I  T KDWE GRLFS QEQIDHK++F+RQEL++  +P++LVGHS+GSYIS+EL 
Sbjct: 71  VIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGSYISLELL 130

Query: 163 RRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLARSPLLSTLFSSFAALLGTLPIQASSFV 222
           ++F D+ VYCIGL+PF+ +N++S +Q  I KLA S +LS   S   A L  LP+ A+  +
Sbjct: 131 KKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLPMSAARLL 190

Query: 223 VKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEF 261
           V K+IG SWS TA +A C+HL +YH+MRNVL+MA +EF
Sbjct: 191 VSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEF 228

The following BLAST results are available for this feature:

BLAST of CSPI06G25760 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5HZX7	1.8e-25	27.88	Lipid droplet-associated hydrolase OS=Rattus norvegicus OX=10116 GN=Ldah PE=2 SV...	[more]
Q54LL8	3.1e-22	28.25	Lipid droplet-associated hydrolase OS=Dictyostelium discoideum OX=44689 GN=DDB_G...	[more]
Q8BVA5	7.7e-21	25.08	Lipid droplet-associated hydrolase OS=Mus musculus OX=10090 GN=Ldah PE=1 SV=1	[more]
Q5F477	1.0e-20	27.64	Lipid droplet-associated hydrolase OS=Gallus gallus OX=9031 GN=LDAH PE=2 SV=2	[more]
Q9H6V9	4.2e-19	27.15	Lipid droplet-associated hydrolase OS=Homo sapiens OX=9606 GN=LDAH PE=1 SV=1	[more]

BLAST of CSPI06G25760 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KFF6	6.5e-196	99.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454400 PE=3 SV=1	[more]
A0A5A7SKD4	1.0e-188	95.91	Lipid droplet-associated hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3CHU9	5.0e-188	95.61	LOW QUALITY PROTEIN: lipid droplet-associated hydrolase OS=Cucumis melo OX=3656 ...	[more]
A0A6J1BTR5	2.9e-156	79.30	lipid droplet-associated hydrolase OS=Momordica charantia OX=3673 GN=LOC11100568...	[more]
A0A6J1I921	3.6e-154	77.21	lipid droplet-associated hydrolase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]

BLAST of CSPI06G25760 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143334.1	1.3e-195	99.71	lipid droplet-associated hydrolase isoform X1 [Cucumis sativus] >KGN48293.1 hypo...	[more]
KAA0025227.1	2.1e-188	95.91	lipid droplet-associated hydrolase [Cucumis melo var. makuwa] >TYK07439.1 lipid ...	[more]
XP_008462607.1	1.0e-187	95.61	PREDICTED: LOW QUALITY PROTEIN: lipid droplet-associated hydrolase [Cucumis melo...	[more]
XP_038881163.1	2.1e-177	90.94	lipid droplet-associated hydrolase [Benincasa hispida]	[more]
XP_011657723.1	1.8e-176	99.68	lipid droplet-associated hydrolase isoform X2 [Cucumis sativus]	[more]

BLAST of CSPI06G25760 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G11620.2	9.8e-104	56.19	alpha/beta-Hydrolases superfamily protein	[more]
AT3G11620.1	9.8e-104	56.19	alpha/beta-Hydrolases superfamily protein	[more]
AT3G11620.3	5.6e-75	58.26	alpha/beta-Hydrolases superfamily protein	[more]
AT3G11620.4	5.6e-75	58.26	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019363	Lipid droplet-associated hydrolase	PFAM	PF10230	LIDHydrolase	coord: 71..319 e-value: 3.3E-64 score: 217.0
IPR019363	Lipid droplet-associated hydrolase	PANTHER	PTHR13390	LIPASE	coord: 44..340
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 50..338 e-value: 4.9E-13 score: 51.3
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 67..322
None	No IPR available	PANTHER	PTHR13390:SF2	BNAA05G27450D PROTEIN	coord: 44..340

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G25760.1	CSPI06G25760.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019915	lipid storage
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005811	lipid droplet
molecular_function	GO:0016298	lipase activity