CSPI06G25380 (gene) Cucumber (PI 183967) v1

BLAST of CSPI06G25380 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.5e-99
Identity = 193/366 (52.73%), Postives = 246/366 (67.21%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFF--TLSLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGN 60
           MD  +KL F  F F  T  LF   G   G  G+    H  +P R TKLFVFGDSY DTGN
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLF---GEINGVEGS-NQNHHLYPFRPTKLFVFGDSYADTGN 60

Query: 61  ILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKE 120
           I   FSS+ +FPYGITFPGKP+GRFSDGRV TDF AK +G+KSPIP+  +   G++RL +
Sbjct: 61  IKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRL-Q 120

Query: 121 SGINFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYS 180
            G+NFA+GGTGVFNT  PLPNMTTQID+F+ +      +    ++  S+ALVSV+GNDYS
Sbjct: 121 YGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNIL-TTGDIYYPPELTSSVALVSVAGNDYS 180

Query: 181 FYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQ 240
            ++A N  A     FI  VV+Q  V+LRRI  LGVKKI V  L PLGCLP FT   SF++
Sbjct: 181 NFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQR 240

Query: 241 CNQTINSFVQFHNFLLKQAVDKLNKQITKHQHYSSSSSSKIFILDVYDAFLSIIQGRGSG 300
           CN+T N+ V  HN LL+Q V KLN +         +  S   ILD+Y+AFL++ + +GS 
Sbjct: 241 CNETQNALVNLHNNLLQQVVAKLNNE---------TKQSTFIILDLYNAFLTVFKNKGSN 300

Query: 301 SGSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTG 360
            GS R     F++PLKPCC GVS E+ CGSVDE+G KK+++C++PK+AFFWD +HPT+ G
Sbjct: 301 PGSTR-----FESPLKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEG 346

Query: 361 WAHAFS 365
           W   +S
Sbjct: 361 WRSVYS 346

BLAST of CSPI06G25380 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 2.0e-86
Identity = 173/361 (47.92%), Postives = 231/361 (63.99%), Query Frame = 0

Query: 6   KLLFTFFFFTL-SLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFS 65
           KLL + F F+  S F G  S    + NL+   R  P+R   LFVFGDSY DTGNI    S
Sbjct: 5   KLLISLFLFSFSSFFLGAESDYPQHSNLK---RLRPNR---LFVFGDSYADTGNIRKSLS 64

Query: 66  SAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFA 125
            + + PYGITFP KPSGRFSDGRV TDF A++LG+KSPIP++ +   G+ERL   G+N+A
Sbjct: 65  DSWKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLL-YGMNYA 124

Query: 126 FGGTGVFNTL-VPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLAT 185
           +GGTGVF T   PLPNMTTQID F+++      + S  D+  SLALVSV+GNDY+ +LA 
Sbjct: 125 YGGTGVFKTKDNPLPNMTTQIDYFQRVL-AAGNIYSPSDLPSSLALVSVAGNDYATFLAL 184

Query: 186 NGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTI 245
                 L  F+  VV+QI V+  RI +LGV KIV+  + PLGCLP  T   SF++CN T 
Sbjct: 185 KRPLTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATD 244

Query: 246 NSFVQFHNFLLKQAVDKLNKQITKHQHYSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGR 305
           N+    HN+LL +A+ +LN +         +  S   +LD Y+AFL++ + +G   G  R
Sbjct: 245 NASTNLHNYLLHKAIARLNNE---------TKPSTFVVLDHYNAFLTVFKNKGPEPGVSR 304

Query: 306 VGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGWAHAF 365
            G      PLKPCC GV+S ++C +VDE+G KK+++C DPK+AFFWD  HP++ GW   +
Sbjct: 305 FG-----NPLKPCCVGVNSSYDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVY 343

BLAST of CSPI06G25380 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 252.7 bits (644), Expect = 6.5e-66
Identity = 146/354 (41.24%), Postives = 205/354 (57.91%), Query Frame = 0

Query: 13  FFTLSLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYG 72
           FF+LSLF G   A G          S  ++  KLFVFG+SY DTGN + P + + + PYG
Sbjct: 8   FFSLSLFFGINGAVG------SNQPSQANKNPKLFVFGNSYADTGN-MKPTALSWKLPYG 67

Query: 73  ITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLK-ESGINFAFGGTGVF 132
           ITFPGKPSGR+SDG   TDF AK LG K  +P+  R+  G++++K   G+NFAFGG+ VF
Sbjct: 68  ITFPGKPSGRYSDGLTATDFLAKQLGAK--LPYLWRTH-GKKKVKLNRGMNFAFGGSEVF 127

Query: 133 NTLVP-LPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGL 192
           ++ V   PN++TQ+     L         + D+  S AL+S SG DY  ++  N +    
Sbjct: 128 DSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFIDQNPNMAAY 187

Query: 193 KPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFH 252
             F+  +V  I   L  +  L  K I VT L PLGCLP  T   SF+ CN++ +  V+ H
Sbjct: 188 PAFVEFIVEDIQYSLGIMNGLKFKNIAVTSLHPLGCLPRVTVASSFRSCNESYSDLVRLH 247

Query: 253 NFLLKQAVDKLNKQITKHQHYSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFK 312
           N  LK+AV KLNK     +    +   +  I+D++ AF++I++ +G+          +FK
Sbjct: 248 NESLKKAVAKLNK-----EDKFRTKGDRFVIVDLHKAFMTILEKKGN---------KRFK 307

Query: 313 TPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGWAHAFS 365
           +PLKPCC G     +C  +D +G KK+ LCNDPKSAFFWD ++PTQ GW   +S
Sbjct: 308 SPLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYS 332

BLAST of CSPI06G25380 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 6.1e-64
Identity = 135/330 (40.91%), Postives = 194/330 (58.79%), Query Frame = 0

Query: 40  PSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGV 99
           P +  KLFVFGDSY DTGN     + A   PYGITFPGKPSGR+ DG + TDF  K LG 
Sbjct: 9   PRKVPKLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGA 68

Query: 100 KSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL--VPLPNMTTQIDLFEQLRDDESGL 159
           +SP  +      G ++  + G+NFAFGG+ + ++    P PN+T Q++    L    +G 
Sbjct: 69  ESPYLYRTH---GRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL--VLAGR 128

Query: 160 ISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIV 219
           +        ++L+S +G DY +Y+  N  A GLK  +  VV+ + V++  +  L  KKI 
Sbjct: 129 VYGDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIA 188

Query: 220 VTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQ---ITKHQHYSSS 279
           VT L P+GCLP +T+  SFK CN++ ++ V+ HN LLK+ V KLN+Q   + K QH+   
Sbjct: 189 VTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHF--- 248

Query: 280 SSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGN 339
                FI+D+++AF+++++ +GS          +FK P+K CC G      CG     G 
Sbjct: 249 -----FIIDIHNAFMTVMKNKGS---------KRFKNPMKSCCEGY-----CGR-SSDGG 308

Query: 340 KKFVLCNDPKSAFFWDSVHPTQTGWAHAFS 365
           K + LC+DPKS FFWD+VHPTQ GW   +S
Sbjct: 309 KLYTLCDDPKSFFFWDAVHPTQEGWRSIYS 309

BLAST of CSPI06G25380 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 238.4 bits (607), Expect = 1.3e-61
Identity = 144/353 (40.79%), Postives = 198/353 (56.09%), Query Frame = 0

Query: 15  TLSLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGIT 74
           ++S  S Q    GF+G           + TKLFVFGDSY DTGN  F    + +FP GIT
Sbjct: 27  SISPSSEQTQEDGFFG----------FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGIT 86

Query: 75  FPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLK-ESGINFAFGGTGVFNT 134
           FPG P+GRFSDGRV TD+ AK++GV++PI +    + G  RL  + G+NFA+GG G F T
Sbjct: 87  FPGIPTGRFSDGRVSTDYLAKYIGVRTPITYK-WGKYGRPRLAVKRGMNFAYGGAGAFET 146

Query: 135 LVPL-PNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKP 194
           +  L P  + QID FEQL      + S  D++ S+A  S+ GNDY  Y   NGS +G   
Sbjct: 147 MFKLVPTASVQIDSFEQLL--MRNVYSPADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSA 206

Query: 195 FINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNF 254
               VV QI++D++RI+ LGV+K++V    P  CLP    P   K C+ T ++    HN 
Sbjct: 207 LTRKVVKQILLDVKRIKDLGVRKVLVALSPPQKCLPKLVTP---KGCD-TNDTSTYLHNS 266

Query: 255 LLKQAVDKLN-KQITKHQHYSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKT 314
           LL++ + KLN K+I       +++      LD+Y+AF++I + +G        G+  F  
Sbjct: 267 LLRKGLIKLNDKEI-------NNNDKSFMTLDLYNAFVTIFKNKGVS------GVSTFPD 326

Query: 315 PLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGWAHAFS 365
           P K CC      F CG     G K + LC+DPKS FFWD+VH +  GW   FS
Sbjct: 327 PFKACCATKRGTF-CGDRSLSGKKLYTLCDDPKSFFFWDNVHISDQGWRSVFS 348

BLAST of CSPI06G25380 vs. ExPASy TrEMBL
Match: A0A5A7SLZ4 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001230 PE=3 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 4.2e-185
Identity = 334/377 (88.59%), Postives = 347/377 (92.04%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFFTLSLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNIL 60
           MDFNLKLLFTFFFFT+SLFSGQGSAGG+Y N RH HRSH  R TKLFVFGDSYVDTGNIL
Sbjct: 1   MDFNLKLLFTFFFFTVSLFSGQGSAGGYYSN-RH-HRSHRLRATKLFVFGDSYVDTGNIL 60

Query: 61  FPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESG 120
            PFSSA+QFPYGITFPGKPSGRFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESG
Sbjct: 61  VPFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESG 120

Query: 121 INFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFY 180
           INFAFGGTGVF+T VPLPNMTTQIDLFEQLR  ESGL SNRDVHLS+ALVSVSGNDYSFY
Sbjct: 121 INFAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGL-SNRDVHLSVALVSVSGNDYSFY 180

Query: 181 LATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN 240
           LATNGSAQGLKPFINSVVNQ+M+DL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN
Sbjct: 181 LATNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN 240

Query: 241 QTINSFVQFHNFLLKQAVDKLNKQITKHQH-------YSSSSSSKIFILDVYDAFLSIIQ 300
           QTINSFVQFHNFLLKQAVDKLNKQIT  QH        SSSSSSKIFILDVYDAFLS+IQ
Sbjct: 241 QTINSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQ 300

Query: 301 GRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVH 360
           GR    GSGRVGLLKFKTPLKPCCFGVSS F CGSVDE+GNKKFVLCNDPKSAFFWDSVH
Sbjct: 301 GR----GSGRVGLLKFKTPLKPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVH 360

Query: 361 PTQTGWAHAFSSFTSFL 371
           PTQTGW+ AFSSFTS L
Sbjct: 361 PTQTGWSLAFSSFTSIL 370

BLAST of CSPI06G25380 vs. ExPASy TrEMBL
Match: A0A1S3CJ29 (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500972 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.8e-175
Identity = 315/358 (87.99%), Postives = 328/358 (91.62%), Query Frame = 0

Query: 20  SGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKP 79
           +GQGSAGG+Y N RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFPGKP
Sbjct: 2   TGQGSAGGYYSN-RH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKP 61

Query: 80  SGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPN 139
           SGRFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T VPLPN
Sbjct: 62  SGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPN 121

Query: 140 MTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVN 199
           MTTQIDLFEQLR  ESGL SNRDVHLS+ALVSVSGNDYSFYLATNGSAQGLKPFINSVVN
Sbjct: 122 MTTQIDLFEQLRHAESGL-SNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVN 181

Query: 200 QIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVD 259
           Q+M+DL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVD
Sbjct: 182 QVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVD 241

Query: 260 KLNKQITKHQH-------YSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTP 319
           KLNKQIT  QH        SSSSSSKIFILDVYDAFLS+IQGR    GSGRVGLLKFKTP
Sbjct: 242 KLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR----GSGRVGLLKFKTP 301

Query: 320 LKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGWAHAFSSFTSFL 371
           LKPCCFGVSS F CGSVDE+GNKKFVLCNDPKSAFFWDSVHPTQTGW+ AFSSFTS L
Sbjct: 302 LKPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 352

BLAST of CSPI06G25380 vs. ExPASy TrEMBL
Match: A0A5D3C686 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001210 PE=3 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 4.4e-174
Identity = 314/356 (88.20%), Postives = 326/356 (91.57%), Query Frame = 0

Query: 22  QGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSG 81
           QGSAGG+Y N RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFPGKPSG
Sbjct: 5   QGSAGGYYSN-RH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSG 64

Query: 82  RFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMT 141
           RFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T VPLPNMT
Sbjct: 65  RFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMT 124

Query: 142 TQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQI 201
           TQIDLFEQLR  ESGL SNRDVHLS+ALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQ+
Sbjct: 125 TQIDLFEQLRHAESGL-SNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQV 184

Query: 202 MVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKL 261
           M+DL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKL
Sbjct: 185 MIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKL 244

Query: 262 NKQITKHQH-------YSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLK 321
           NKQIT  QH        SSSSSSKIFILDVYDAFLS+IQGR    GSGRVGLLKFKTPLK
Sbjct: 245 NKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR----GSGRVGLLKFKTPLK 304

Query: 322 PCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGWAHAFSSFTSFL 371
           PCCFGVSS F CGSVDE+GNKKFVLCNDPKSAFFWDSVHPTQTGW+ AFSSFTS L
Sbjct: 305 PCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 353

BLAST of CSPI06G25380 vs. ExPASy TrEMBL
Match: A0A6J1ECT1 (GDSL esterase/lipase At5g03610-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 9.0e-135
Identity = 250/371 (67.39%), Postives = 289/371 (77.90%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFFTLSLFSGQGSAGGFYGNLRH--RHRSHPSRGTKLFVFGDSYVDTGN 60
           MDF L+LLF+     +SLFSG+GSAGG Y +  H  RH  H  R TKLFVFGDSY DTGN
Sbjct: 1   MDFVLRLLFSLLLL-ISLFSGRGSAGGVYNHHHHRRRHLRHRFRPTKLFVFGDSYADTGN 60

Query: 61  ILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKE 120
           ILFP S A QFPYGITFPGKP GRFSDGRVLTDFAA+H+G+KSPIPFS+R+ VG  R KE
Sbjct: 61  ILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKE 120

Query: 121 SGINFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYS 180
           +G+NFAFGGTGVF+T  PLPNMTTQID F+QLR  +S + +N D   S+ALVS SGNDY+
Sbjct: 121 TGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDS-IFTNTDAQSSVALVSASGNDYA 180

Query: 181 FYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQ 240
           FY A N S QGLKPFI SVV+QI+V+L+RI  LGVKKI VTGLGPLGCLP+FTAP S+KQ
Sbjct: 181 FYFAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQ 240

Query: 241 CNQTINSFVQFHNFLLKQAVDKLNKQITKHQHYSSSSSSKIFILDVYDAFLSIIQGRGSG 300
           CN+TINS VQFHN LLK+AV KLN++ TKH       SSK+F++DVY AF+SII+GR   
Sbjct: 241 CNETINSLVQFHNLLLKEAVKKLNQE-TKH-------SSKVFVVDVYGAFMSIIEGR--- 300

Query: 301 SGSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTG 360
               R    +FKTPLKPCC+GV S F CGSVD +G K FVLC D K+AFFWDSVHPTQ G
Sbjct: 301 ---KRYNNGEFKTPLKPCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDSVHPTQQG 355

Query: 361 WAHAFSSFTSF 370
           WA A S+F SF
Sbjct: 361 WAAAISAFKSF 355

BLAST of CSPI06G25380 vs. ExPASy TrEMBL
Match: A0A6J1EIS2 (GDSL esterase/lipase At5g03610-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 9.0e-135
Identity = 250/371 (67.39%), Postives = 289/371 (77.90%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFFTLSLFSGQGSAGGFYGNLRH--RHRSHPSRGTKLFVFGDSYVDTGN 60
           MDF L+LLF+     +SLFSG+GSAGG Y +  H  RH  H  R TKLFVFGDSY DTGN
Sbjct: 2   MDFVLRLLFSLLLL-ISLFSGRGSAGGVYNHHHHRRRHLRHRFRPTKLFVFGDSYADTGN 61

Query: 61  ILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKE 120
           ILFP S A QFPYGITFPGKP GRFSDGRVLTDFAA+H+G+KSPIPFS+R+ VG  R KE
Sbjct: 62  ILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKE 121

Query: 121 SGINFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYS 180
           +G+NFAFGGTGVF+T  PLPNMTTQID F+QLR  +S + +N D   S+ALVS SGNDY+
Sbjct: 122 TGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDS-IFTNTDAQSSVALVSASGNDYA 181

Query: 181 FYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQ 240
           FY A N S QGLKPFI SVV+QI+V+L+RI  LGVKKI VTGLGPLGCLP+FTAP S+KQ
Sbjct: 182 FYFAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQ 241

Query: 241 CNQTINSFVQFHNFLLKQAVDKLNKQITKHQHYSSSSSSKIFILDVYDAFLSIIQGRGSG 300
           CN+TINS VQFHN LLK+AV KLN++ TKH       SSK+F++DVY AF+SII+GR   
Sbjct: 242 CNETINSLVQFHNLLLKEAVKKLNQE-TKH-------SSKVFVVDVYGAFMSIIEGR--- 301

Query: 301 SGSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTG 360
               R    +FKTPLKPCC+GV S F CGSVD +G K FVLC D K+AFFWDSVHPTQ G
Sbjct: 302 ---KRYNNGEFKTPLKPCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDSVHPTQQG 356

Query: 361 WAHAFSSFTSF 370
           WA A S+F SF
Sbjct: 362 WAAAISAFKSF 356

BLAST of CSPI06G25380 vs. NCBI nr
Match: XP_004143379.2 (GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48533.2 hypothetical protein Csa_003217 [Cucumis sativus])

HSP 1 Score: 732.3 bits (1889), Expect = 2.1e-207
Identity = 365/371 (98.38%), Postives = 365/371 (98.38%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFFTLSLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNIL 60
           MDFNLKLLFTFFFFTLSLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNIL
Sbjct: 1   MDFNLKLLFTFFFFTLSLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNIL 60

Query: 61  FPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESG 120
           FPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESG
Sbjct: 61  FPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESG 120

Query: 121 INFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFY 180
           INFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFY
Sbjct: 121 INFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFY 180

Query: 181 LATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN 240
           LATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN
Sbjct: 181 LATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN 240

Query: 241 QTINSFVQFHNFLLKQAVDKLNKQITKHQHY-SSSSSSKIFILDVYDAFLSIIQGRGSGS 300
           QTINSFVQFHNFLLKQAVDKLNKQITK QHY SSSSSSKIFILDVYDAFLSIIQGR    
Sbjct: 241 QTINSFVQFHNFLLKQAVDKLNKQITKQQHYSSSSSSSKIFILDVYDAFLSIIQGR---- 300

Query: 301 GSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGW 360
           GSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGW
Sbjct: 301 GSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGW 360

Query: 361 AHAFSSFTSFL 371
           AHAFSSFTSFL
Sbjct: 361 AHAFSSFTSFL 367

BLAST of CSPI06G25380 vs. NCBI nr
Match: KAA0025271.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 657.1 bits (1694), Expect = 8.8e-185
Identity = 334/377 (88.59%), Postives = 347/377 (92.04%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFFTLSLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNIL 60
           MDFNLKLLFTFFFFT+SLFSGQGSAGG+Y N RH HRSH  R TKLFVFGDSYVDTGNIL
Sbjct: 1   MDFNLKLLFTFFFFTVSLFSGQGSAGGYYSN-RH-HRSHRLRATKLFVFGDSYVDTGNIL 60

Query: 61  FPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESG 120
            PFSSA+QFPYGITFPGKPSGRFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESG
Sbjct: 61  VPFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESG 120

Query: 121 INFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFY 180
           INFAFGGTGVF+T VPLPNMTTQIDLFEQLR  ESGL SNRDVHLS+ALVSVSGNDYSFY
Sbjct: 121 INFAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGL-SNRDVHLSVALVSVSGNDYSFY 180

Query: 181 LATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN 240
           LATNGSAQGLKPFINSVVNQ+M+DL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN
Sbjct: 181 LATNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN 240

Query: 241 QTINSFVQFHNFLLKQAVDKLNKQITKHQH-------YSSSSSSKIFILDVYDAFLSIIQ 300
           QTINSFVQFHNFLLKQAVDKLNKQIT  QH        SSSSSSKIFILDVYDAFLS+IQ
Sbjct: 241 QTINSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQ 300

Query: 301 GRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVH 360
           GR    GSGRVGLLKFKTPLKPCCFGVSS F CGSVDE+GNKKFVLCNDPKSAFFWDSVH
Sbjct: 301 GR----GSGRVGLLKFKTPLKPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVH 360

Query: 361 PTQTGWAHAFSSFTSFL 371
           PTQTGW+ AFSSFTS L
Sbjct: 361 PTQTGWSLAFSSFTSIL 370

BLAST of CSPI06G25380 vs. NCBI nr
Match: XP_008462669.1 (PREDICTED: GDSL esterase/lipase At5g03610-like, partial [Cucumis melo])

HSP 1 Score: 623.2 bits (1606), Expect = 1.4e-174
Identity = 315/358 (87.99%), Postives = 328/358 (91.62%), Query Frame = 0

Query: 20  SGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKP 79
           +GQGSAGG+Y N RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFPGKP
Sbjct: 2   TGQGSAGGYYSN-RH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKP 61

Query: 80  SGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPN 139
           SGRFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T VPLPN
Sbjct: 62  SGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPN 121

Query: 140 MTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVN 199
           MTTQIDLFEQLR  ESGL SNRDVHLS+ALVSVSGNDYSFYLATNGSAQGLKPFINSVVN
Sbjct: 122 MTTQIDLFEQLRHAESGL-SNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVN 181

Query: 200 QIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVD 259
           Q+M+DL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVD
Sbjct: 182 QVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVD 241

Query: 260 KLNKQITKHQH-------YSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTP 319
           KLNKQIT  QH        SSSSSSKIFILDVYDAFLS+IQGR    GSGRVGLLKFKTP
Sbjct: 242 KLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR----GSGRVGLLKFKTP 301

Query: 320 LKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGWAHAFSSFTSFL 371
           LKPCCFGVSS F CGSVDE+GNKKFVLCNDPKSAFFWDSVHPTQTGW+ AFSSFTS L
Sbjct: 302 LKPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 352

BLAST of CSPI06G25380 vs. NCBI nr
Match: TYK07397.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 620.5 bits (1599), Expect = 9.1e-174
Identity = 314/356 (88.20%), Postives = 326/356 (91.57%), Query Frame = 0

Query: 22  QGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSG 81
           QGSAGG+Y N RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFPGKPSG
Sbjct: 5   QGSAGGYYSN-RH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSG 64

Query: 82  RFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMT 141
           RFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T VPLPNMT
Sbjct: 65  RFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMT 124

Query: 142 TQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQI 201
           TQIDLFEQLR  ESGL SNRDVHLS+ALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQ+
Sbjct: 125 TQIDLFEQLRHAESGL-SNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQV 184

Query: 202 MVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKL 261
           M+DL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKL
Sbjct: 185 MIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKL 244

Query: 262 NKQITKHQH-------YSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLK 321
           NKQIT  QH        SSSSSSKIFILDVYDAFLS+IQGR    GSGRVGLLKFKTPLK
Sbjct: 245 NKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR----GSGRVGLLKFKTPLK 304

Query: 322 PCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGWAHAFSSFTSFL 371
           PCCFGVSS F CGSVDE+GNKKFVLCNDPKSAFFWDSVHPTQTGW+ AFSSFTS L
Sbjct: 305 PCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 353

BLAST of CSPI06G25380 vs. NCBI nr
Match: XP_038883594.1 (GDSL esterase/lipase At5g03610-like [Benincasa hispida])

HSP 1 Score: 590.5 bits (1521), Expect = 1.0e-164
Identity = 304/372 (81.72%), Postives = 325/372 (87.37%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFFTLSLFSGQG--SAGGFYG-NLRHRHRSHPSRGTKLFVFGDSYVDTG 60
           M+FNL LLF+FFFF +SLFSGQG  SAG +YG +  H HR H  R TKLFVFGDSYVDTG
Sbjct: 1   MNFNLSLLFSFFFFMISLFSGQGSASAGRYYGDHHHHHHRRHRLRPTKLFVFGDSYVDTG 60

Query: 61  NILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLK 120
           NIL PFSSA+QFPYGITFPGKPSGRFSDGRVLTDF AKHLGVKSPIPFSIRSEVG ERLK
Sbjct: 61  NILLPFSSAQQFPYGITFPGKPSGRFSDGRVLTDFLAKHLGVKSPIPFSIRSEVGVERLK 120

Query: 121 ESGINFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDY 180
           ESGINFAFGGTGVF+T VPLPNMTTQID F+ LR  ES + SN D   S+ALVSVSGNDY
Sbjct: 121 ESGINFAFGGTGVFDTSVPLPNMTTQIDFFQHLRHVES-VFSNGDAQHSVALVSVSGNDY 180

Query: 181 SFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFK 240
           SFYLATNGSAQGLKPFINSVVNQI++DL+RIRRLGVKKIVVTGLGPLGCLPIFTAPFSFK
Sbjct: 181 SFYLATNGSAQGLKPFINSVVNQIVIDLKRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFK 240

Query: 241 QCNQTINSFVQFHNFLLKQAVDKLNKQITKHQHYSSSSSSKIFILDVYDAFLSIIQGRGS 300
           QCNQTINSFV+FHNFLL QAV KLNK+ TKH    +SSSSKIF+LDVYDAFLSIIQ R  
Sbjct: 241 QCNQTINSFVRFHNFLLNQAVAKLNKE-TKH----ASSSSKIFVLDVYDAFLSIIQSRSK 300

Query: 301 GSGSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQT 360
             G     +L+FKTPLKPCCFGVSS FECGSVDEQGNKKFVLCN+PKSAFFWDSVHPTQT
Sbjct: 301 KPGV----VLEFKTPLKPCCFGVSSGFECGSVDEQGNKKFVLCNNPKSAFFWDSVHPTQT 360

Query: 361 GWAHAFSSFTSF 370
           GW+ AFSSF SF
Sbjct: 361 GWSVAFSSFKSF 362

BLAST of CSPI06G25380 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.1e-100
Identity = 193/366 (52.73%), Postives = 246/366 (67.21%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFF--TLSLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGN 60
           MD  +KL F  F F  T  LF   G   G  G+    H  +P R TKLFVFGDSY DTGN
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLF---GEINGVEGS-NQNHHLYPFRPTKLFVFGDSYADTGN 60

Query: 61  ILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKE 120
           I   FSS+ +FPYGITFPGKP+GRFSDGRV TDF AK +G+KSPIP+  +   G++RL +
Sbjct: 61  IKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRL-Q 120

Query: 121 SGINFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYS 180
            G+NFA+GGTGVFNT  PLPNMTTQID+F+ +      +    ++  S+ALVSV+GNDYS
Sbjct: 121 YGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNIL-TTGDIYYPPELTSSVALVSVAGNDYS 180

Query: 181 FYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQ 240
            ++A N  A     FI  VV+Q  V+LRRI  LGVKKI V  L PLGCLP FT   SF++
Sbjct: 181 NFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQR 240

Query: 241 CNQTINSFVQFHNFLLKQAVDKLNKQITKHQHYSSSSSSKIFILDVYDAFLSIIQGRGSG 300
           CN+T N+ V  HN LL+Q V KLN +         +  S   ILD+Y+AFL++ + +GS 
Sbjct: 241 CNETQNALVNLHNNLLQQVVAKLNNE---------TKQSTFIILDLYNAFLTVFKNKGSN 300

Query: 301 SGSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTG 360
            GS R     F++PLKPCC GVS E+ CGSVDE+G KK+++C++PK+AFFWD +HPT+ G
Sbjct: 301 PGSTR-----FESPLKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEG 346

Query: 361 WAHAFS 365
           W   +S
Sbjct: 361 WRSVYS 346

BLAST of CSPI06G25380 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 320.9 bits (821), Expect = 1.4e-87
Identity = 173/361 (47.92%), Postives = 231/361 (63.99%), Query Frame = 0

Query: 6   KLLFTFFFFTL-SLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFS 65
           KLL + F F+  S F G  S    + NL+   R  P+R   LFVFGDSY DTGNI    S
Sbjct: 5   KLLISLFLFSFSSFFLGAESDYPQHSNLK---RLRPNR---LFVFGDSYADTGNIRKSLS 64

Query: 66  SAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFA 125
            + + PYGITFP KPSGRFSDGRV TDF A++LG+KSPIP++ +   G+ERL   G+N+A
Sbjct: 65  DSWKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLL-YGMNYA 124

Query: 126 FGGTGVFNTL-VPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLAT 185
           +GGTGVF T   PLPNMTTQID F+++      + S  D+  SLALVSV+GNDY+ +LA 
Sbjct: 125 YGGTGVFKTKDNPLPNMTTQIDYFQRVL-AAGNIYSPSDLPSSLALVSVAGNDYATFLAL 184

Query: 186 NGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTI 245
                 L  F+  VV+QI V+  RI +LGV KIV+  + PLGCLP  T   SF++CN T 
Sbjct: 185 KRPLTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATD 244

Query: 246 NSFVQFHNFLLKQAVDKLNKQITKHQHYSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGR 305
           N+    HN+LL +A+ +LN +         +  S   +LD Y+AFL++ + +G   G  R
Sbjct: 245 NASTNLHNYLLHKAIARLNNE---------TKPSTFVVLDHYNAFLTVFKNKGPEPGVSR 304

Query: 306 VGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGWAHAF 365
            G      PLKPCC GV+S ++C +VDE+G KK+++C DPK+AFFWD  HP++ GW   +
Sbjct: 305 FG-----NPLKPCCVGVNSSYDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVY 343

BLAST of CSPI06G25380 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 4.3e-65
Identity = 135/330 (40.91%), Postives = 194/330 (58.79%), Query Frame = 0

Query: 40  PSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGV 99
           P +  KLFVFGDSY DTGN     + A   PYGITFPGKPSGR+ DG + TDF  K LG 
Sbjct: 9   PRKVPKLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGA 68

Query: 100 KSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL--VPLPNMTTQIDLFEQLRDDESGL 159
           +SP  +      G ++  + G+NFAFGG+ + ++    P PN+T Q++    L    +G 
Sbjct: 69  ESPYLYRTH---GRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL--VLAGR 128

Query: 160 ISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIV 219
           +        ++L+S +G DY +Y+  N  A GLK  +  VV+ + V++  +  L  KKI 
Sbjct: 129 VYGDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIA 188

Query: 220 VTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQ---ITKHQHYSSS 279
           VT L P+GCLP +T+  SFK CN++ ++ V+ HN LLK+ V KLN+Q   + K QH+   
Sbjct: 189 VTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHF--- 248

Query: 280 SSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGN 339
                FI+D+++AF+++++ +GS          +FK P+K CC G      CG     G 
Sbjct: 249 -----FIIDIHNAFMTVMKNKGS---------KRFKNPMKSCCEGY-----CGR-SSDGG 308

Query: 340 KKFVLCNDPKSAFFWDSVHPTQTGWAHAFS 365
           K + LC+DPKS FFWD+VHPTQ GW   +S
Sbjct: 309 KLYTLCDDPKSFFFWDAVHPTQEGWRSIYS 309

BLAST of CSPI06G25380 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 238.4 bits (607), Expect = 9.1e-63
Identity = 144/353 (40.79%), Postives = 198/353 (56.09%), Query Frame = 0

Query: 15  TLSLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGIT 74
           ++S  S Q    GF+G           + TKLFVFGDSY DTGN  F    + +FP GIT
Sbjct: 27  SISPSSEQTQEDGFFG----------FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGIT 86

Query: 75  FPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLK-ESGINFAFGGTGVFNT 134
           FPG P+GRFSDGRV TD+ AK++GV++PI +    + G  RL  + G+NFA+GG G F T
Sbjct: 87  FPGIPTGRFSDGRVSTDYLAKYIGVRTPITYK-WGKYGRPRLAVKRGMNFAYGGAGAFET 146

Query: 135 LVPL-PNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKP 194
           +  L P  + QID FEQL      + S  D++ S+A  S+ GNDY  Y   NGS +G   
Sbjct: 147 MFKLVPTASVQIDSFEQLL--MRNVYSPADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSA 206

Query: 195 FINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNF 254
               VV QI++D++RI+ LGV+K++V    P  CLP    P   K C+ T ++    HN 
Sbjct: 207 LTRKVVKQILLDVKRIKDLGVRKVLVALSPPQKCLPKLVTP---KGCD-TNDTSTYLHNS 266

Query: 255 LLKQAVDKLN-KQITKHQHYSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKT 314
           LL++ + KLN K+I       +++      LD+Y+AF++I + +G        G+  F  
Sbjct: 267 LLRKGLIKLNDKEI-------NNNDKSFMTLDLYNAFVTIFKNKGVS------GVSTFPD 326

Query: 315 PLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGWAHAFS 365
           P K CC      F CG     G K + LC+DPKS FFWD+VH +  GW   FS
Sbjct: 327 PFKACCATKRGTF-CGDRSLSGKKLYTLCDDPKSFFFWDNVHISDQGWRSVFS 348

BLAST of CSPI06G25380 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 144.4 bits (363), Expect = 1.8e-34
Identity = 93/303 (30.69%), Postives = 137/303 (45.21%), Query Frame = 0

Query: 62  PFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGI 121
           P + + + PYGITFPGKPSGR+SDG   TDF              + SEV +  +  S  
Sbjct: 3   PTALSWKLPYGITFPGKPSGRYSDGLTATDF--------------LGSEVFDSPVDRS-- 62

Query: 122 NFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYL 181
                           PN++TQ+     L         + D+  S AL+S SG DY  ++
Sbjct: 63  ----------------PNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFI 122

Query: 182 ATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQ 241
             N +      F+  +V  I                                     CN+
Sbjct: 123 DQNPNMAAYPAFVEFIVEDI------------------------------------HCNE 182

Query: 242 TINSFVQFHNFLLKQAVDKLNKQITKHQHYSSSSSSKIFILDVYDAFLSIIQGRGSGSGS 301
           + +  V+ HN  LK+AV KLNK     +    +   +  I+D++ AF++I++ +G+    
Sbjct: 183 SYSDLVRLHNESLKKAVAKLNK-----EDKFRTKGDRFVIVDLHKAFMTILEKKGN---- 218

Query: 302 GRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGWAH 361
                 +FK+PLKPCC G     +C  +D +G KK+ LCNDPKSAFFWD ++PTQ GW  
Sbjct: 243 -----KRFKSPLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRS 218

Query: 362 AFS 365
            +S
Sbjct: 303 IYS 218