CSPI06G25010 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G25010
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	L-threonine dehydratase
Location	Chr6: 22304530 .. 22304772 (+)
RNA-Seq Expression	CSPI06G25010
Synteny	CSPI06G25010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGGCTAATCTTTCTAAAGAGGAGCTTAAAAATGGAGTTGTTTGTGCATCAACTGGTGGAGACCATGCACAAGCAGCCCTTGCTTTGGCTGCCAACAAAATAGGAATTACTGAGATCGCCATTGTTATGCCTTCTACAACCCCTTCAATAAAGGTGCAAACTCTTTTCTTCGTTTTAGCTAAAACCCTCCACTACTTGTTAGAAAGAGAAATGGTGTGGATAACACAAAAGTGTGGAGAG

mRNA sequence

ATGATGGCTAATCTTTCTAAAGAGGAGCTTAAAAATGGAGTTGTTTGTGCATCAACTGGTGGAGACCATGCACAAGCAGCCCTTGCTTTGGCTGCCAACAAAATAGGAATTACTGAGATCGCCATTGTTATGCCTTCTACAACCCCTTCAATAAAGGTGCAAACTCTTTTCTTCGTTTTAGCTAAAACCCTCCACTACTTGTTAGAAAGAGAAATGGTGTGGATAACACAAAAGTGTGGAGAG

Coding sequence (CDS)

ATGATGGCTAATCTTTCTAAAGAGGAGCTTAAAAATGGAGTTGTTTGTGCATCAACTGGTGGAGACCATGCACAAGCAGCCCTTGCTTTGGCTGCCAACAAAATAGGAATTACTGAGATCGCCATTGTTATGCCTTCTACAACCCCTTCAATAAAGGTGCAAACTCTTTTCTTCGTTTTAGCTAAAACCCTCCACTACTTGTTAGAAAGAGAAATGGTGTGGATAACACAAAAGTGTGGAGAG

Protein sequence

MMANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIKVQTLFFVLAKTLHYLLEREMVWITQKCGE

Homology

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BLAST of CSPI06G25010 vs. ExPASy Swiss-Prot
Match: A0FKE6 (Threonine dehydratase 1 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4081 GN=TD1 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 5.3e-05
Identity = 28/52 (53.85%), Postives = 38/52 (73.08%), Query Frame = 0


Query: 1   MMANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIK 53
           MMA L KE+L+ GV+C S+ G+HAQ  +AL+A ++G  +  IVMP TTP IK
Sbjct: 161 MMAKLPKEQLEKGVIC-SSAGNHAQ-GVALSAQRLG-CDAVIVMPVTTPDIK 209

[Close]

BLAST of CSPI06G25010 vs. ExPASy Swiss-Prot
Match: P25306 (Threonine dehydratase 2 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4081 GN=TD2 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 3.5e-04
Identity = 28/56 (50.00%), Postives = 38/56 (67.86%), Query Frame = 0


Query: 1   MMANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIKVQTL 57
           MM+NLS+EEL  GV+ AS  G+HAQ  +ALA  ++      IVMP+TTP IK+  +
Sbjct: 150 MMSNLSREELDKGVITAS-AGNHAQ-GVALAGQRLNCV-AKIVMPTTTPQIKIDAV 202

[Close]

BLAST of CSPI06G25010 vs. ExPASy Swiss-Prot
Match: Q9AXU4 (Threonine dehydratase OS=Nicotiana attenuata OX=49451 GN=TD PE=1 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 5.9e-04
Identity = 28/56 (50.00%), Postives = 38/56 (67.86%), Query Frame = 0


Query: 1   MMANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIKVQTL 57
           MM  LSKE+L+ GV+ AS  G+HAQ  +AL A ++  T   IVMP TTP IK++ +
Sbjct: 157 MMTKLSKEQLERGVITAS-AGNHAQ-GVALGAQRLKCT-ATIVMPVTTPEIKIEAV 209

[Close]

BLAST of CSPI06G25010 vs. ExPASy TrEMBL
Match: C7RJQ5 (L-threonine dehydratase OS=Accumulibacter phosphatis (strain UW-1) OX=522306 GN=ilvA PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.2e-05
Identity = 33/55 (60.00%), Postives = 43/55 (78.18%), Query Frame = 0


Query: 2   MANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIKVQTL 57
           + +LSKE+LK GV+CAS  G+HAQ  +ALAANK+G   + IVMP+TTP IKVQ +
Sbjct: 59  IVHLSKEKLKRGVICAS-AGNHAQ-GVALAANKVGCRAV-IVMPTTTPQIKVQAV 110

[Close]

BLAST of CSPI06G25010 vs. ExPASy TrEMBL
Match: A0A7X6WN68 (Threonine ammonia-lyase, biosynthetic OS=Oxalobacter sp. OX=2093374 GN=ilvA PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.2e-04
Identity = 30/55 (54.55%), Postives = 45/55 (81.82%), Query Frame = 0


Query: 2   MANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIKVQTL 57
           MA+LS+++LKNGV+CAS  G+HAQ  +ALAAN++G   + IVMP+TTP +K++ +
Sbjct: 59  MAHLSRKQLKNGVICAS-AGNHAQ-GVALAANRLGCRAM-IVMPTTTPPVKIEAV 110

[Close]

BLAST of CSPI06G25010 vs. ExPASy TrEMBL
Match: A0A4Q3U2V9 (Pyridoxal-phosphate dependent enzyme (Fragment) OS=bacterium OX=1869227 GN=EON82_20740 PE=4 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.6e-04
Identity = 32/55 (58.18%), Postives = 42/55 (76.36%), Query Frame = 0


Query: 2  MANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIKVQTL 57
          MANLS E+L  GV+CAS  G+HAQ  +ALAA K+G   + IVMP+TTPSIK++ +
Sbjct: 34 MANLSPEQLAKGVICAS-AGNHAQ-GVALAARKLGCRAV-IVMPNTTPSIKIEAV 85

[Close]

BLAST of CSPI06G25010 vs. ExPASy TrEMBL
Match: A0A2G2VU03 (Threonine dehydratase OS=Capsicum baccatum OX=33114 GN=CQW23_24157 PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.8e-04
Identity = 31/52 (59.62%), Postives = 41/52 (78.85%), Query Frame = 0


Query: 1  MMANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIK 53
          MMANLS+E+LK GV+C S+ G+HAQ  +ALA++K+G   + IVMP TTP IK
Sbjct: 1  MMANLSEEQLKRGVIC-SSAGNHAQ-GVALASHKLGCNAV-IVMPVTTPQIK 49

[Close]

BLAST of CSPI06G25010 vs. ExPASy TrEMBL
Match: A0A2G2YLM1 (PALP domain-containing protein OS=Capsicum annuum OX=4072 GN=T459_25751 PE=4 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.8e-04
Identity = 31/52 (59.62%), Postives = 41/52 (78.85%), Query Frame = 0


Query: 1   MMANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIK 53
           MMANLS+E+LK GV+C S+ G+HAQ  +ALA++K+G   + IVMP TTP IK
Sbjct: 75  MMANLSEEQLKRGVIC-SSAGNHAQ-GVALASHKLGCNAV-IVMPVTTPQIK 123

[Close]

BLAST of CSPI06G25010 vs. NCBI nr
Match: XP_038882927.1 (threonine dehydratase 1 biosynthetic, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 70.5 bits (171), Expect = 7.7e-09
Identity = 40/54 (74.07%), Postives = 44/54 (81.48%), Query Frame = 0


Query: 1   MMANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIKVQ 55
           MMANL KEEL  GV+ ASTGGDHAQ  LALAANK+G TE  IVMP+TTP IKV+
Sbjct: 194 MMANLPKEELDKGVIFASTGGDHAQ-GLALAANKLG-TETVIVMPTTTPPIKVE 245

[Close]

BLAST of CSPI06G25010 vs. NCBI nr
Match: KAF3663063.1 (hypothetical protein FXO38_10829 [Capsicum annuum])

HSP 1 Score: 58.9 bits (141), Expect = 2.3e-05
Identity = 36/65 (55.38%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 1   MMANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIKVQTLFFVL 60
           MMANLSKE+LK GV+C S+ G+HAQ  +AL ++K+G   + IVMP TTP IKV+T F  L
Sbjct: 47  MMANLSKEQLKRGVIC-SSAGNHAQ-GVALTSHKLGCNAV-IVMPVTTPQIKVRT-FVTL 106

Query: 61  AKTLH 66
              +H
Sbjct: 107 LSFMH 107

[Close]

BLAST of CSPI06G25010 vs. NCBI nr
Match: MBL8422642.1 (threonine ammonia-lyase, biosynthetic [Candidatus Accumulibacter phosphatis])

HSP 1 Score: 57.0 bits (136), Expect = 8.8e-05
Identity = 33/55 (60.00%), Postives = 43/55 (78.18%), Query Frame = 0


Query: 2   MANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIKVQTL 57
           + +LSKE+LK GV+CAS  G+HAQ  +ALAANK+G   + IVMP+TTP IKVQ +
Sbjct: 59  IVHLSKEKLKRGVICAS-AGNHAQ-GVALAANKVGCRAV-IVMPTTTPQIKVQAV 110

[Close]

BLAST of CSPI06G25010 vs. NCBI nr
Match: WP_012807069.1 (threonine ammonia-lyase, biosynthetic [Candidatus Accumulibacter phosphatis] >MBN8495793.1 threonine ammonia-lyase, biosynthetic [Candidatus Accumulibacter sp.] >MBO3713637.1 threonine ammonia-lyase, biosynthetic [Candidatus Accumulibacter sp.])

HSP 1 Score: 57.0 bits (136), Expect = 8.8e-05
Identity = 33/55 (60.00%), Postives = 43/55 (78.18%), Query Frame = 0


Query: 2   MANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIKVQTL 57
           + +LSKE+LK GV+CAS  G+HAQ  +ALAANK+G   + IVMP+TTP IKVQ +
Sbjct: 59  IVHLSKEKLKRGVICAS-AGNHAQ-GVALAANKVGCRAV-IVMPTTTPQIKVQAV 110

[Close]

BLAST of CSPI06G25010 vs. NCBI nr
Match: NQW80975.1 (threonine ammonia-lyase, biosynthetic [Polaromonas sp.])

HSP 1 Score: 55.8 bits (133), Expect = 2.0e-04
Identity = 32/55 (58.18%), Postives = 41/55 (74.55%), Query Frame = 0


Query: 2   MANLSKEELKNGVVCASTGGDHAQAALALAANKIGITEIAIVMPSTTPSIKVQTL 57
           MANLS  +LK GV+CAS  G+HAQ  +AL+ANK+G T   IVMPSTTP +K+  +
Sbjct: 73  MANLSPAQLKKGVICAS-AGNHAQ-GVALSANKLG-TRAVIVMPSTTPQLKIDAV 124

The following BLAST results are available for this feature:

BLAST of CSPI06G25010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0FKE6	5.3e-05	53.85	Threonine dehydratase 1 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4...	[more]
P25306	3.5e-04	50.00	Threonine dehydratase 2 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4...	[more]
Q9AXU4	5.9e-04	50.00	Threonine dehydratase OS=Nicotiana attenuata OX=49451 GN=TD PE=1 SV=1	[more]

BLAST of CSPI06G25010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
C7RJQ5	4.2e-05	60.00	L-threonine dehydratase OS=Accumulibacter phosphatis (strain UW-1) OX=522306 GN=...	[more]
A0A7X6WN68	1.2e-04	54.55	Threonine ammonia-lyase, biosynthetic OS=Oxalobacter sp. OX=2093374 GN=ilvA PE=4...	[more]
A0A4Q3U2V9	1.6e-04	58.18	Pyridoxal-phosphate dependent enzyme (Fragment) OS=bacterium OX=1869227 GN=EON82...	[more]
A0A2G2VU03	2.8e-04	59.62	Threonine dehydratase OS=Capsicum baccatum OX=33114 GN=CQW23_24157 PE=3 SV=1	[more]
A0A2G2YLM1	2.8e-04	59.62	PALP domain-containing protein OS=Capsicum annuum OX=4072 GN=T459_25751 PE=4 SV=...	[more]

BLAST of CSPI06G25010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038882927.1	7.7e-09	74.07	threonine dehydratase 1 biosynthetic, chloroplastic-like [Benincasa hispida]	[more]
KAF3663063.1	2.3e-05	55.38	hypothetical protein FXO38_10829 [Capsicum annuum]	[more]
MBL8422642.1	8.8e-05	60.00	threonine ammonia-lyase, biosynthetic [Candidatus Accumulibacter phosphatis]	[more]
WP_012807069.1	8.8e-05	60.00	threonine ammonia-lyase, biosynthetic [Candidatus Accumulibacter phosphatis] >MB...	[more]
NQW80975.1	2.0e-04	58.18	threonine ammonia-lyase, biosynthetic [Polaromonas sp.]	[more]

BLAST of CSPI06G25010 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 1..56 e-value: 2.5E-11 score: 45.3
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 4..56

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G25010.1	CSPI06G25010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009097	isoleucine biosynthetic process
biological_process	GO:0006567	threonine catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004794	L-threonine ammonia-lyase activity
molecular_function	GO:0030170	pyridoxal phosphate binding