CSPI06G22820 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G22820
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial
LocationChr6: 20602923 .. 20608193 (+)
RNA-Seq ExpressionCSPI06G22820
SyntenyCSPI06G22820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAATATTTGGCCAATTTTATTTAAAATTATCCTAAGGCACCGTATATATTAAGTAGGCTTCTTCGTCTTCTCCACGCTCTGTGTTCAGCGGCGAATATGCTATCCACACTGCAACCCCCTCGCTGCTCTCTCTCTCGATTCACTCTCCATCTTCCCTCCAAACCTTCCTTCTTTCACTCCAAACAAACCCCACTTTCAACTCCACATTCCACTCTTACAGACCAACTTCCCACTTCCAATTCTTCCCAACCCCTTCAGTCCCAAATTCTCTCCATCCGCCATTCACTTCTTTCCCGCCAAATCACTGCCACCCAACTCGCCGATTCCTATCTCAATCGCCTTCGTGCTTTGGAGCCTCACCTCAAGTCATTTCTCCACGTCTCTGAAACTGTCCGCTCTGATGCCCGTGAGATTGATGACAAAATTCTCAGAAATGAGGAACTGGGTCCTTTAGCTGGGGTGCTTGTGGCCGTTAAGGACAATATTTGTACTGCGGGTATGCCTTCCACTGCTGGGTCTCGGATTCTTGATGGATATCGCCCACCATTTGACGCTACTGCTGTGAAGAGAATCAAGGAGTTGGGTGGGATTGTGATTGGGAAGACGAATTTGGATGAATTTGGCATGGGTAGCTCTACTGAAGCCTCTGCTTTCCAGGTTTTCGTTCTCTTTAATATATATAACTGTCCTCTGCTTATTATCATATTGTGGGGGCTGGAAGTGTGTGGGTGCGGTTTTTTGTTTTTCTCAGTATTACTTTTTGTCATGAAAATGAGGTTAAAGTAGACACTTCAATGGGGTTTGAGCATGTTGTACTTATTTGGTAAAAGTGTGGCTGTGTGGTGTTACTTCCCACTGGTAACAGGGGTTGGTTTACTCAGGTGGTGAGTCTGTTCATTCAATAGGTGACTGCAAATCCGTGGGATTTGAGTAGGGTCCCGGGAGGATCATCAGGAGGATCTGCTTCAGCTGTTTCTGCTAGGCAGTGTGTGGTGTCATTGGGAAGTGATACAGGTGGAAGTGTGCGGCAGCCAGCATCTTTTTGCGGTGTTGTTGGGTTGAAACCAACATATGGTCGTGTCTCAAGATTTGGACTTATGGCCTATGCTTCTTCGCTTGATGTTATTGGCTGCTTGAGTACAACAGTTGCTGATGCTGGGATTCTTCTACATGCAATTTCTGGTCATGATACCCTTGATGCCACTAGTAGTAAACGAGTAAGGACTTTCTTGCTTTGTTCCATTCTTTTTTTTTTTTTTGAACTCCTTTGCTATTGTTTAAGATTTATATCGTTTTACACACTTGAGCTTGAGATAGAAACGTTAGTTTATGCCTGTTTCTTGGGAAATTTAAAGTTGTTAAGTAACTTTGGTAGTCACATGTTGCTGTTTTTGATTGTTCATGAGGATTTTTTCTTTAGTGCTCAGGTAAGCAAGCTGCTTGAATTTAGAAACTGTAGAATCAGATAATGAATTATTTTTATATCTTCTGGAGTAGTTTCAATATATGGTGCATTGTTTTAGCATTTTCTTATTTGTTTTTGTTCATGCCTTCCAGGAGGTTTCTGATTTCACATCACAATTTTCTGCCGTTGATTCATTCGAATCTAAACCCTTGAGAGGATTGAGGATTGGCCTGATTCGTGAAACCCTTGATAAGGGTGTCGATGGTCAAGTAAATTCTGCTATACGTGCTGCTGCTTCACATCTAGAAGAGTTAGGATGTTCCATCAATGAGGTTTCTATTTTCTTTTATTTTTCATTGCTTTTTGTGCTTTACTTCCTAGTCGTCTTGATTTTTTTTTCTCCATTCATTCCCTTCTTAGAAAAACTTATTCTCTTGTAGGTATCACTACCATCATTTTCTCTTGGGCTGCCAGCGTATTATATTCTTGCATCATCTGAATCATCTTCAAACTTAGCACGATATGATGGTGTCAGGTATCCTGCTGAAGTCGATGTTTGTTATAAAAAATATTTTAGTGTTTTCTTGAAATAAAAAGATTCTAATATCCCTTTGAGTTTATTGACGTTCAGGTTTAAGAATTTTCCTTAGCGTACTTTCTCAGCTCATGATATTGAATCCTCTGAATGGTTATTTGGTTCTCTTATGTTGAAGAAACCATTTAATTACCTAAATAATTTTTCAGGTGCTTTTGTTTTGTTACTGTATTGTGATAACTTATTCTGATTCAATTTGGCCACCGTAACCTACATATAGATATGGAAATCAAGCTATTGCTGATGAGCTAACTGGTCTATATGAAAATTCCCGAGCAACAGGATTTGGTTCAGAGGTACTAGTTGGAAAATGTTAAAAAATTTATCTTGCATTGAAATTGGACATTTTACTTTCTTATTCACTTTGTAAAGGTTAAATTCTTTTATTTGGTTGAGTTGTGATATATGTATACTAGAATTTTAACCTTATCCTCTTTCCTTTTGGGAAATTGACAACAAATCAGTGACATTTACATTTTCTCATTGCAGGTTAAAATGAGAATTTTGATGGGAACATATGCTCTCTCAGCTGGTTATTATGATGCTTACTACAAGCGTGCTCAGCAGGTGACATGCTTATTCTTGGATCCCCCCTTTCCTCACGAATAATAAGATAATAATAATGAAAAGCAACATGAAACAAATAGTGAACATTGATTAGTGTCTTAGAACAGTAGTTACTGAACGTGATGTCTATTTCCAGGAAGCTCATATGTCAGTGACAATTGTTATGGAACCTTACTCGAAATGTTGGATAAAAGCTAACCATCATGTGTTGACCTAGTGATAAAAAAGGAGACAGTCTTAATAACTAATTTAGAGGTTGTGAGTTCAATCCATAGTGACTACCTACTTAGAAGTTAATTTCCTACGAGTTTCCTTTGACACCCAAATATTGTAGAATCAGGTAGGCTGTCCCATGAGATTAGTCGAGATACGCGTAAGCAGACCCCAAACACTCGTGGATATCAATAAAAAAAAGTTGGATAAAAGCTTAACGGTTCTTTGTGATTTCTTATTAAAATAAAATAATAATTATAAGGGCTCTTCATAATTCCAACGGTGTTATTAGGTGAGAACAATTATCCAGAAAAGCTTCAGAGCAGCACTAGATGAACATGACATCTTAATATCACCTGCTGCACCATCTGCCGCTTATAAAATTGGTATGCCTTTGCCTTCCAAAATTTTGTGTCTGTCAATTTGGTGTACATTACTTATTGATCATTGCTTGTCAACAGGTGAAAAGGTGGATGACCCATTGGCAATGTATGCAGGTGACATCATGACTGTAAGCATTTCTACTCCCCTTTGTTTGAGTACTTTCCGATTTAATATCTAGGCTTGTATATGTAGAGAAATTATTAGTGGTAAGGAATCCGAATCTTGAAATAGAGCTCTCTCATTAAAGAAACCTGGTTAGTTAGTCCTAATATTTCTACTGTTAGTTAGAGTTTTTGTTTATATAACAGAGAAATCAACTATCACGTTTATAGATTTACATTCAGTGACATTCCCCAGTAACTAATTGGTCATTTTCACTATGCACAACCACTCCATTGTTCTTGGTTCTCAACTTTTCAACATTGACATAAATACCCACTGTCAAGGATTTTTACAAATTAATTGTGAGGCGAGAATATGCTCCAAAAGCATCGCTGTCTTGACGAACCTTTTATGTTTGAGATGATTAACACGATGATATATTATTCTTGTTCCAAGGAGTCCATCTAAATCCCCAGTCCATATTGCCTTATCCATTAGACTGCCTTGATACGTCTGCCACTGAAACAAAAAGAAATGTGATAATGGATCTCTTTGCCTTGGACCTTTTGAATCTTTGAAGGACGCTTCTTGGTTTACTATTATAATTAAGAAAAGAGAATTATGTATCGGAAGTCAAAACTACATCCATAAGTCCACGCTCTTCACATTGTGCGCCTGCTGTAAAAAACTTACTTGTAAAGAACTTCCTTTAGAAAATTTCAATTTTATTGTGGGCTGTCTTGACATTGATATCCTTTTTCTTCCAAACAATGCAATTAATCATCTCACAATTTCGTTAGCTATAAGCACTAATTCTAAAAGTTCACGCTTTTATTTTCCTCCTTTATATGTTACATCCTCAACAGGTCAATGTTAACTTGGCTGGACTACCTGCATTGGTATTGCCTTGTGGATTTGTTCAAGATGGGTCCTCTAACCTTCCTGTTGGCCTTCAAATGATTGGTGCAGCTTTTGATGAGGTACTGTTTAACCTATTGCACAGCATGTTACTTTTAATTACTGCTGAGTGCTGAGATTGATTGTACTTCTATACATCTTTGGTTAAAATCACTTTGTCATGTTTAAAAATGCTCCAAAACATGTCTTTAATGAACTCAATCCAATTAAAATTGATTTTGAATTTTTAAATGCACATTCTAAATTGATTTTAAACATGACAAAAGCAATCACTCTCGAACATGCCCTTTGATTCTTTGCCCGGTTAATTCCCGTTGAGGTAGGGTGTGTCTGGAATACATCTCCAAGTGTTTAATTTAAAAAAATAAGTCTTTAAGGAAAATGTGGGAGTGTTTGACAACCAATACTTTTGAATTGTATATAAACAATTTTCTGGAGAGTAAATACAAATGAGAAAAAAGTATAAGTCAATTCAAATGGACTAGTAATGTAACCCAAATACTTCATTGAAAAAAAATAAAAGGATAATGCAATGCGGACGATTATTAAGGAGATTAAGAACAAATTAGTTCATGTTTGTTTGTTTCTTCTTTTAGTACAAATTGGAAGTACCTTTGAACCAAGGACCTTTTGATCCTCATTAGATGAGTAGCATGTTTGTTTGCTTGTTGATGCCACGGTAGTACAGAGTTCATTGTGGAGAACGAGAACTTTAGAACTCTGAATCATTGACAATTTACATCATGCTTACAGGGCAAACTTCTTAAAGTGGGACACATCTTCGAACAAACTCTTGCCGACTGCAGATTTGTTCCACCCTTACTAGCTGACGACATTGTAGGTTAGCTCCAAATCAGTTGTGTTGTGAAAGCAGGCAAAATTGTAAGTCCGGGAATGCTACAATTGATTGATATTGTATTATTGATTATTATTAGATGAGCATTACTAGCTGGAGGAATTATTATTTTTTTGTATTTTCTCTTATATATTAGAACAAATGGATTGTGTTTTCCATTATCTATATGACAATAAAGAGAGCAAAATTTTGTTTGCTATTGGGAGCTAATTAAACTTAGTCTTAACT

mRNA sequence

AAAAAATATTTGGCCAATTTTATTTAAAATTATCCTAAGGCACCGTATATATTAAGTAGGCTTCTTCGTCTTCTCCACGCTCTGTGTTCAGCGGCGAATATGCTATCCACACTGCAACCCCCTCGCTGCTCTCTCTCTCGATTCACTCTCCATCTTCCCTCCAAACCTTCCTTCTTTCACTCCAAACAAACCCCACTTTCAACTCCACATTCCACTCTTACAGACCAACTTCCCACTTCCAATTCTTCCCAACCCCTTCAGTCCCAAATTCTCTCCATCCGCCATTCACTTCTTTCCCGCCAAATCACTGCCACCCAACTCGCCGATTCCTATCTCAATCGCCTTCGTGCTTTGGAGCCTCACCTCAAGTCATTTCTCCACGTCTCTGAAACTGTCCGCTCTGATGCCCGTGAGATTGATGACAAAATTCTCAGAAATGAGGAACTGGGTCCTTTAGCTGGGGTGCTTGTGGCCGTTAAGGACAATATTTGTACTGCGGGTATGCCTTCCACTGCTGGGTCTCGGATTCTTGATGGATATCGCCCACCATTTGACGCTACTGCTGTGAAGAGAATCAAGGAGTTGGGTGGGATTGTGATTGGGAAGACGAATTTGGATGAATTTGGCATGGGTAGCTCTACTGAAGCCTCTGCTTTCCAGGTGACTGCAAATCCGTGGGATTTGAGTAGGGTCCCGGGAGGATCATCAGGAGGATCTGCTTCAGCTGTTTCTGCTAGGCAGTGTGTGGTGTCATTGGGAAGTGATACAGGTGGAAGTGTGCGGCAGCCAGCATCTTTTTGCGGTGTTGTTGGGTTGAAACCAACATATGGTCGTGTCTCAAGATTTGGACTTATGGCCTATGCTTCTTCGCTTGATGTTATTGGCTGCTTGAGTACAACAGTTGCTGATGCTGGGATTCTTCTACATGCAATTTCTGGTCATGATACCCTTGATGCCACTAGTAGTAAACGAGAGGTTTCTGATTTCACATCACAATTTTCTGCCGTTGATTCATTCGAATCTAAACCCTTGAGAGGATTGAGGATTGGCCTGATTCGTGAAACCCTTGATAAGGGTGTCGATGGTCAAGTAAATTCTGCTATACGTGCTGCTGCTTCACATCTAGAAGAGTTAGGATGTTCCATCAATGAGGTATCACTACCATCATTTTCTCTTGGGCTGCCAGCGTATTATATTCTTGCATCATCTGAATCATCTTCAAACTTAGCACGATATGATGGTGTCAGATATGGAAATCAAGCTATTGCTGATGAGCTAACTGGTCTATATGAAAATTCCCGAGCAACAGGATTTGGTTCAGAGGTTAAAATGAGAATTTTGATGGGAACATATGCTCTCTCAGCTGGTTATTATGATGCTTACTACAAGCGTGCTCAGCAGGTGAGAACAATTATCCAGAAAAGCTTCAGAGCAGCACTAGATGAACATGACATCTTAATATCACCTGCTGCACCATCTGCCGCTTATAAAATTGGTGAAAAGGTGGATGACCCATTGGCAATGTATGCAGGTGACATCATGACTGTCAATGTTAACTTGGCTGGACTACCTGCATTGGTATTGCCTTGTGGATTTGTTCAAGATGGGTCCTCTAACCTTCCTGTTGGCCTTCAAATGATTGGTGCAGCTTTTGATGAGGGCAAACTTCTTAAAGTGGGACACATCTTCGAACAAACTCTTGCCGACTGCAGATTTGTTCCACCCTTACTAGCTGACGACATTGTAGGTTAGCTCCAAATCAGTTGTGTTGTGAAAGCAGGCAAAATTGTAAGTCCGGGAATGCTACAATTGATTGATATTGTATTATTGATTATTATTAGATGAGCATTACTAGCTGGAGGAATTATTATTTTTTTGTATTTTCTCTTATATATTAGAACAAATGGATTGTGTTTTCCATTATCTATATGACAATAAAGAGAGCAAAATTTTGTTTGCTATTGGGAGCTAATTAAACTTAGTCTTAACT

Coding sequence (CDS)

ATGCTATCCACACTGCAACCCCCTCGCTGCTCTCTCTCTCGATTCACTCTCCATCTTCCCTCCAAACCTTCCTTCTTTCACTCCAAACAAACCCCACTTTCAACTCCACATTCCACTCTTACAGACCAACTTCCCACTTCCAATTCTTCCCAACCCCTTCAGTCCCAAATTCTCTCCATCCGCCATTCACTTCTTTCCCGCCAAATCACTGCCACCCAACTCGCCGATTCCTATCTCAATCGCCTTCGTGCTTTGGAGCCTCACCTCAAGTCATTTCTCCACGTCTCTGAAACTGTCCGCTCTGATGCCCGTGAGATTGATGACAAAATTCTCAGAAATGAGGAACTGGGTCCTTTAGCTGGGGTGCTTGTGGCCGTTAAGGACAATATTTGTACTGCGGGTATGCCTTCCACTGCTGGGTCTCGGATTCTTGATGGATATCGCCCACCATTTGACGCTACTGCTGTGAAGAGAATCAAGGAGTTGGGTGGGATTGTGATTGGGAAGACGAATTTGGATGAATTTGGCATGGGTAGCTCTACTGAAGCCTCTGCTTTCCAGGTGACTGCAAATCCGTGGGATTTGAGTAGGGTCCCGGGAGGATCATCAGGAGGATCTGCTTCAGCTGTTTCTGCTAGGCAGTGTGTGGTGTCATTGGGAAGTGATACAGGTGGAAGTGTGCGGCAGCCAGCATCTTTTTGCGGTGTTGTTGGGTTGAAACCAACATATGGTCGTGTCTCAAGATTTGGACTTATGGCCTATGCTTCTTCGCTTGATGTTATTGGCTGCTTGAGTACAACAGTTGCTGATGCTGGGATTCTTCTACATGCAATTTCTGGTCATGATACCCTTGATGCCACTAGTAGTAAACGAGAGGTTTCTGATTTCACATCACAATTTTCTGCCGTTGATTCATTCGAATCTAAACCCTTGAGAGGATTGAGGATTGGCCTGATTCGTGAAACCCTTGATAAGGGTGTCGATGGTCAAGTAAATTCTGCTATACGTGCTGCTGCTTCACATCTAGAAGAGTTAGGATGTTCCATCAATGAGGTATCACTACCATCATTTTCTCTTGGGCTGCCAGCGTATTATATTCTTGCATCATCTGAATCATCTTCAAACTTAGCACGATATGATGGTGTCAGATATGGAAATCAAGCTATTGCTGATGAGCTAACTGGTCTATATGAAAATTCCCGAGCAACAGGATTTGGTTCAGAGGTTAAAATGAGAATTTTGATGGGAACATATGCTCTCTCAGCTGGTTATTATGATGCTTACTACAAGCGTGCTCAGCAGGTGAGAACAATTATCCAGAAAAGCTTCAGAGCAGCACTAGATGAACATGACATCTTAATATCACCTGCTGCACCATCTGCCGCTTATAAAATTGGTGAAAAGGTGGATGACCCATTGGCAATGTATGCAGGTGACATCATGACTGTCAATGTTAACTTGGCTGGACTACCTGCATTGGTATTGCCTTGTGGATTTGTTCAAGATGGGTCCTCTAACCTTCCTGTTGGCCTTCAAATGATTGGTGCAGCTTTTGATGAGGGCAAACTTCTTAAAGTGGGACACATCTTCGAACAAACTCTTGCCGACTGCAGATTTGTTCCACCCTTACTAGCTGACGACATTGTAGGTTAG

Protein sequence

MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSIRHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLAGVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSSTEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQFSAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLGLPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYALSAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDIMTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFVPPLLADDIVG*
Homology
BLAST of CSPI06G22820 vs. ExPASy Swiss-Prot
Match: E0CTY1 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Vitis vinifera OX=29760 GN=GATA PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 1.6e-225
Identity = 415/550 (75.45%), Postives = 462/550 (84.00%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTP--LSTPHSTLTDQLPTSNSSQPLQSQIL 60
           MLST+Q PR  L R   H P K   FHS  TP  LS P +  +   P+S    P QSQIL
Sbjct: 1   MLSTVQNPR-FLLRQCRHFPPKRRRFHS-STPLFLSQPQTLASTDPPSS----PPQSQIL 60

Query: 61  SIRHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGP 120
           +IR +L++R+ TA +LA   LNRLR  EP + SF+ VSETV  +A EID KI RNEELGP
Sbjct: 61  TIRDNLINRRTTAVELAQLSLNRLRLTEPQINSFISVSETVLREAEEIDAKIARNEELGP 120

Query: 121 LAGVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMG 180
           LAGV V VKDNICTA MPST GSRIL+ YRPPFDATAVKR+K+ GGIV+GKTNLDEFGMG
Sbjct: 121 LAGVFVGVKDNICTADMPSTGGSRILENYRPPFDATAVKRVKDCGGIVVGKTNLDEFGMG 180

Query: 181 SSTEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVG 240
           S+TE SAFQVTANPWD+SRVPGGSSGGSA+AVSARQCVVSLGSDTGGSVRQPASFCGVVG
Sbjct: 181 STTEGSAFQVTANPWDVSRVPGGSSGGSAAAVSARQCVVSLGSDTGGSVRQPASFCGVVG 240

Query: 241 LKPTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTS 300
           LKPTYGRVSRFGLMAYASSLDVIGC  ++VAD GILLHAI+G+D LD+TSSK EV ++TS
Sbjct: 241 LKPTYGRVSRFGLMAYASSLDVIGCFGSSVADTGILLHAIAGYDGLDSTSSKHEVPNYTS 300

Query: 301 QFSAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFS 360
           QF ++ S ESKPL+GLR+GLIRETLD GVD  V S+I  AASHLEELGC++ EVSLPSFS
Sbjct: 301 QFMSISSLESKPLKGLRVGLIRETLDDGVDKAVVSSIVGAASHLEELGCTVTEVSLPSFS 360

Query: 361 LGLPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTY 420
           LGLPAYYILASSESSSNL+RYDGVRYG+Q  AD L  LY +SRA GFG EVKMRIL GTY
Sbjct: 361 LGLPAYYILASSESSSNLSRYDGVRYGSQVGADRLNSLYGDSRAKGFGPEVKMRILTGTY 420

Query: 421 ALSAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAG 480
           ALSAGYYDAYYKRAQQVRT++QKSF+AAL+E+DILISPAAPS AY IGEK +DPLAMYAG
Sbjct: 421 ALSAGYYDAYYKRAQQVRTLVQKSFKAALNENDILISPAAPSPAYSIGEKKNDPLAMYAG 480

Query: 481 DIMTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCR 540
           DIMTVNVNLAGLPALVLPCGFV+ G + LPVGLQMIGAAFDE KLLKVGHIFEQ L   +
Sbjct: 481 DIMTVNVNLAGLPALVLPCGFVEGGPAGLPVGLQMIGAAFDEEKLLKVGHIFEQMLKGYK 540

Query: 541 FVPPLLADDI 549
           F+PPLLAD I
Sbjct: 541 FIPPLLADGI 544

BLAST of CSPI06G22820 vs. ExPASy Swiss-Prot
Match: Q9LI77 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GATA PE=2 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 6.0e-225
Identity = 412/546 (75.46%), Postives = 461/546 (84.43%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60
           MLSTLQPPR SLS   L        F   +T +S   S   D   TS  S P QSQIL+ 
Sbjct: 1   MLSTLQPPR-SLSLLPLRR------FQISKTIVSAASSKTID---TSVISPP-QSQILTT 60

Query: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120
           R SLLS + TA ++A SYL+R+R  EP LK FLHVSE V  DA+EID +I + EELGPLA
Sbjct: 61  RRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEIDQRIAKGEELGPLA 120

Query: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180
           GVL+ VKDNICT GMPSTA SRIL+ YRPPFDATAVK+IKELGGIV+GKTN+DEFGMGS+
Sbjct: 121 GVLIGVKDNICTQGMPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEFGMGST 180

Query: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240
           TEASAFQVTANPWDLSRVPGGSSGGSA+AV+ARQC+VSLGSDTGGSVRQPASFCGVVGLK
Sbjct: 181 TEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLK 240

Query: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300
           PTYGRVSRFGLMAYASSLDVIGC  +TVADAG+LLHAISG+D  D+TSSK++V +F SQF
Sbjct: 241 PTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSSKQDVPEFQSQF 300

Query: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360
            +VD FESKPL G+++G+IRETL+ GVD  V SA + AASHLE LGC + EVSLPSFSLG
Sbjct: 301 LSVDHFESKPLNGVKVGIIRETLEDGVDSGVRSATQEAASHLEALGCILTEVSLPSFSLG 360

Query: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420
           LPAYY++ASSESSSNL+RYDGVRYGNQ +A+EL  LYE SR  GFG EVKMRILMGTYAL
Sbjct: 361 LPAYYVIASSESSSNLSRYDGVRYGNQVMAEELNKLYECSRGEGFGGEVKMRILMGTYAL 420

Query: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480
           SAGYYDAYYKRAQQVRT+I+K F+AAL+++DILISPAAPSAAYKIGEK DDPLAMYAGDI
Sbjct: 421 SAGYYDAYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAGDI 480

Query: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540
           MTVNVNLAGLPA+VLPCG V+ G S LPVGLQMIGAAFDE KLLKVGHIFEQTL    FV
Sbjct: 481 MTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLKGSSFV 535

Query: 541 PPLLAD 547
           PPLLA+
Sbjct: 541 PPLLAN 535

BLAST of CSPI06G22820 vs. ExPASy Swiss-Prot
Match: B6U151 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Zea mays OX=4577 GN=GATA PE=2 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 5.1e-200
Identity = 364/545 (66.79%), Postives = 434/545 (79.63%), Query Frame = 0

Query: 5   LQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSIRHSL 64
           LQ  R  +S   L  P++  F         T  S++     T+ +  P  S ILSIR SL
Sbjct: 5   LQAHRLLISHRRLPTPARRRF---------TAASSVQSAPATTLAPGPATSSILSIRESL 64

Query: 65  LSRQITATQLADSYLNRLRALEPHLKSFLHVSE-TVRSDAREIDDKILRNEE--LGPLAG 124
           LS + TA  +   YL+RLR  EP L+SF+HV++     +A E+D +I   E+  +GPLAG
Sbjct: 65  LSGERTAADITSEYLSRLRRTEPTLRSFIHVADAAAEREAEELDRRIASGEKDAVGPLAG 124

Query: 125 VLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSST 184
           VLV VKDN+CTA MPST GSRILDGYRP +DATAV+R++E G IV+GKTNLDEFGMGS+T
Sbjct: 125 VLVGVKDNLCTANMPSTGGSRILDGYRPAYDATAVRRLREAGAIVLGKTNLDEFGMGSTT 184

Query: 185 EASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKP 244
           E SAFQVT NPWD SRVPGGSSGGSA+AVSARQCVVSLGSDTGGSVRQPASFCGVVGLKP
Sbjct: 185 EGSAFQVTTNPWDDSRVPGGSSGGSAAAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKP 244

Query: 245 TYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQFS 304
           TYGRVSRFGLMAYASSLDV+GC  ++V D   +L  ++GHD +DATSS + V ++ S+  
Sbjct: 245 TYGRVSRFGLMAYASSLDVVGCFGSSVFDTATILSVVAGHDKMDATSSSQVVPEYASELV 304

Query: 305 AVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLGL 364
           ++D  ESKPL G+RIG+I+ETL +GV   V S+I+ AASHLE+LG  + EVSLPSFSLGL
Sbjct: 305 SLDLLESKPLNGVRIGIIQETLGEGVASGVVSSIKGAASHLEQLGSVVEEVSLPSFSLGL 364

Query: 365 PAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYALS 424
           PAYYILASSE+SSNL+RYDG+RYG Q  AD+L  LY  SRA GFG EVKMRILMGTYALS
Sbjct: 365 PAYYILASSEASSNLSRYDGIRYGGQVSADDLNELYGESRANGFGHEVKMRILMGTYALS 424

Query: 425 AGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDIM 484
           AGYYDAYYKRAQQVRT++++SF+ AL+ +DILISPAAPSAAYKIGEK++DPLAMYAGDIM
Sbjct: 425 AGYYDAYYKRAQQVRTLVKQSFKDALERYDILISPAAPSAAYKIGEKINDPLAMYAGDIM 484

Query: 485 TVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFVP 544
           TVNVNLAGLPALV+PCGFV+ G++ LPVGLQM+G+ F EG LL+VGHIFEQTL +  FVP
Sbjct: 485 TVNVNLAGLPALVVPCGFVEGGAAGLPVGLQMMGSPFCEGNLLRVGHIFEQTLQNLSFVP 540

Query: 545 PLLAD 547
           PLLA+
Sbjct: 545 PLLAE 540

BLAST of CSPI06G22820 vs. ExPASy Swiss-Prot
Match: C5Y8Z8 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Sorghum bicolor OX=4558 GN=GATA PE=3 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 1.5e-199
Identity = 365/545 (66.97%), Postives = 432/545 (79.27%), Query Frame = 0

Query: 5   LQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSIRHSL 64
           LQ  R  +S   L  P++  F         T  S+L     T+ +  P  S ILSIR SL
Sbjct: 5   LQAHRLLISHRRLPSPARRRF---------TAASSLQSAPATTLAPGPATSSILSIRESL 64

Query: 65  LSRQITATQLADSYLNRLRALEPHLKSFLHVSE-TVRSDAREIDDKILRNEE--LGPLAG 124
           LS + TA  +   YL+RLR  EP L+SF+HV++     +A E+D +I   E+  +GPLAG
Sbjct: 65  LSGERTAADITSEYLSRLRRTEPSLRSFIHVADAAAEREAEELDRRIASGEKDAVGPLAG 124

Query: 125 VLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSST 184
           VLV VKDN+CTA MPST GSRILDGYRP +DATAV+R++E G IV+GKTNLDEFGMGS+T
Sbjct: 125 VLVGVKDNLCTANMPSTGGSRILDGYRPAYDATAVRRLQEAGAIVVGKTNLDEFGMGSTT 184

Query: 185 EASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKP 244
           E SAFQVT NPWD SRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKP
Sbjct: 185 EGSAFQVTTNPWDDSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKP 244

Query: 245 TYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQFS 304
           TYGRVSRFGLMAYASSLDV+GC  ++V D   +L  ++GHD +D+TSS + V D+ S+  
Sbjct: 245 TYGRVSRFGLMAYASSLDVVGCFGSSVFDTATILSVVAGHDKMDSTSSSQVVPDYASELV 304

Query: 305 AVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLGL 364
           ++D  ESKPL GLRIG+I+ETL +GV   V S+I+ AASHLE+LG  + EVSLPSFSLGL
Sbjct: 305 SLDLLESKPLAGLRIGIIQETLGEGVANGVISSIKGAASHLEQLGSVVEEVSLPSFSLGL 364

Query: 365 PAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYALS 424
           PAYYILASSE+SSNL+RYDG+RYG Q  AD+L  LY  SRA G G EVKMRILMGTYALS
Sbjct: 365 PAYYILASSEASSNLSRYDGIRYGRQFSADDLNELYGESRANGLGHEVKMRILMGTYALS 424

Query: 425 AGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDIM 484
           AGYYDAYYKRAQQVRT++++SF+ AL+ +DILISPAAPSAAYKIGEK++DPLAMYAGDI+
Sbjct: 425 AGYYDAYYKRAQQVRTLVKESFKDALERYDILISPAAPSAAYKIGEKINDPLAMYAGDIL 484

Query: 485 TVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFVP 544
           TVNVNLAGLPALV+PCGFV+ G + LPVGLQ+IG+ F EG LL+VGHIFEQTL +  FVP
Sbjct: 485 TVNVNLAGLPALVVPCGFVEGGPAGLPVGLQLIGSPFCEGNLLRVGHIFEQTLQNLSFVP 540

Query: 545 PLLAD 547
           PLLA+
Sbjct: 545 PLLAE 540

BLAST of CSPI06G22820 vs. ExPASy Swiss-Prot
Match: Q25A68 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=GATA PE=3 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 5.7e-199
Identity = 360/543 (66.30%), Postives = 430/543 (79.19%), Query Frame = 0

Query: 7   PPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSIRHSLLS 66
           PP     R  L     PS    + T +S+  S+    +  + +  P  S ILSIR SLLS
Sbjct: 2   PPPLQAQRLLLSHRRLPSPHRRRFTAVSSLPSSPAKTVAAAAAHAP--SSILSIRESLLS 61

Query: 67  RQITATQLADSYLNRLRALEPHLKSFLHVSE-TVRSDAREIDDKILRN--EELGPLAGVL 126
            + TA ++   YL+RLR  EP ++SF+HV++     +A E+D +I     + +GPLAGVL
Sbjct: 62  GERTAAEITAEYLSRLRRTEPSVRSFIHVADAAAEREAEELDRRIATEGLDAVGPLAGVL 121

Query: 127 VAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSSTEA 186
           V VKDN+CTA MPST GSRILDGY+P +DATAV+R++E G IV+GKTNLDEFGMGS+TE 
Sbjct: 122 VGVKDNLCTANMPSTGGSRILDGYQPAYDATAVRRLREAGAIVVGKTNLDEFGMGSTTEG 181

Query: 187 SAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKPTY 246
           S FQVT NPWD SRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKPTY
Sbjct: 182 SGFQVTTNPWDDSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKPTY 241

Query: 247 GRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQFSAV 306
           GRVSRFGLMAYASSLDV+GC  ++V D   +L  I+GHD +D+TSS  +VSD+ S+   +
Sbjct: 242 GRVSRFGLMAYASSLDVVGCFGSSVVDTATILSVIAGHDKMDSTSSSHDVSDYKSELVPL 301

Query: 307 DSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLGLPA 366
           D  ESKPL G+RIG+I+ETL +GV+  V S+I+ AASHLE+LG  + EVSLPSFSLGLPA
Sbjct: 302 DLLESKPLNGMRIGIIQETLGEGVETGVISSIKDAASHLEQLGSVVEEVSLPSFSLGLPA 361

Query: 367 YYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYALSAG 426
           YYILASSE+SSNL+RYDG+RYG Q   D+L  LY  SRA G G EVKMRILMGTYALSAG
Sbjct: 362 YYILASSEASSNLSRYDGIRYGRQVSGDDLNELYGGSRANGLGHEVKMRILMGTYALSAG 421

Query: 427 YYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDIMTV 486
           YYDAYYKRAQQVRT+++KSF+ AL+ +DIL+SPAAPSAAYKIGEK++DPLAMYAGD MTV
Sbjct: 422 YYDAYYKRAQQVRTLVKKSFKEALERYDILVSPAAPSAAYKIGEKINDPLAMYAGDTMTV 481

Query: 487 NVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFVPPL 546
           NVNLAGLPALV+PCGFV+ GS+ LPVGLQMIG+ F EG LL++GHIFEQTL +  FVPPL
Sbjct: 482 NVNLAGLPALVVPCGFVEGGSAGLPVGLQMIGSPFSEGNLLRIGHIFEQTLQNYSFVPPL 541

BLAST of CSPI06G22820 vs. ExPASy TrEMBL
Match: A0A0A0KEN8 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cucumis sativus OX=3659 GN=GATA PE=3 SV=1)

HSP 1 Score: 1059.7 bits (2739), Expect = 4.2e-306
Identity = 547/550 (99.45%), Postives = 549/550 (99.82%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60
           MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI
Sbjct: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60

Query: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120
           RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA
Sbjct: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120

Query: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180
           GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS
Sbjct: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180

Query: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240
           TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK
Sbjct: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240

Query: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300
           PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLH+ISGHDTLDATSSKREVSDFTSQF
Sbjct: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHSISGHDTLDATSSKREVSDFTSQF 300

Query: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360
           SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG
Sbjct: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360

Query: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420
           LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL
Sbjct: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420

Query: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480
           SAGYYDAYYKRAQQVRTIIQKSFRAALDE+DILISP APSAAYKIGEKVDDPLAMYAGDI
Sbjct: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEYDILISPTAPSAAYKIGEKVDDPLAMYAGDI 480

Query: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540
           MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV
Sbjct: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540

Query: 541 PPLLADDIVG 551
           PPLLADDIVG
Sbjct: 541 PPLLADDIVG 550

BLAST of CSPI06G22820 vs. ExPASy TrEMBL
Match: A0A1S3BM64 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC103491372 PE=3 SV=1)

HSP 1 Score: 1055.8 bits (2729), Expect = 6.1e-305
Identity = 545/550 (99.09%), Postives = 549/550 (99.82%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60
           MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI
Sbjct: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60

Query: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120
           RHSLLSRQITATQLADSYL+RLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA
Sbjct: 61  RHSLLSRQITATQLADSYLDRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120

Query: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180
           GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVK+IKELGGIVIGKTNLDEFGMGSS
Sbjct: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKKIKELGGIVIGKTNLDEFGMGSS 180

Query: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240
           TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK
Sbjct: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240

Query: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300
           PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF
Sbjct: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300

Query: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360
           SAVDSFESKPL+GLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG
Sbjct: 301 SAVDSFESKPLKGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360

Query: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420
           LPAYYILASSESSSNLARYDGVRYGNQA ADELTGLYENSRATGFGSEVKMRILMGTYAL
Sbjct: 361 LPAYYILASSESSSNLARYDGVRYGNQATADELTGLYENSRATGFGSEVKMRILMGTYAL 420

Query: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480
           SAGYYDAYYKRAQQVRTIIQKSFRAALDE+DILISPAAPSAAYKIGEKVDDPLAMYAGDI
Sbjct: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480

Query: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540
           MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV
Sbjct: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540

Query: 541 PPLLADDIVG 551
           PPLLADDIVG
Sbjct: 541 PPLLADDIVG 550

BLAST of CSPI06G22820 vs. ExPASy TrEMBL
Match: A0A5D3DC36 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=GATA PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.6e-289
Identity = 524/550 (95.27%), Postives = 528/550 (96.00%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60
           MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI
Sbjct: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60

Query: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120
           RHSLLSRQITATQLADSYL+RLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA
Sbjct: 61  RHSLLSRQITATQLADSYLDRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120

Query: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180
           GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVK+IKELGGIVIGKTNLDEFGMGSS
Sbjct: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKKIKELGGIVIGKTNLDEFGMGSS 180

Query: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240
           TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK
Sbjct: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240

Query: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300
           PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF
Sbjct: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300

Query: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360
           SAVDSFESKPL+GLRIGLIRETLDKGVDGQ                     VSLPSFSLG
Sbjct: 301 SAVDSFESKPLKGLRIGLIRETLDKGVDGQ---------------------VSLPSFSLG 360

Query: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420
           LPAYYILASSESSSNLARYDGVRYGNQA ADELTGLYENSRATGFGSEVKMRILMGTYAL
Sbjct: 361 LPAYYILASSESSSNLARYDGVRYGNQATADELTGLYENSRATGFGSEVKMRILMGTYAL 420

Query: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480
           SAGYYDAYYKRAQQVRTIIQKSFRAALDE+DILISPAAPSAAYKIGEKVDDPLAMYAGDI
Sbjct: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480

Query: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540
           MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV
Sbjct: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 529

Query: 541 PPLLADDIVG 551
           PPLLADDIVG
Sbjct: 541 PPLLADDIVG 529

BLAST of CSPI06G22820 vs. ExPASy TrEMBL
Match: A0A6J1HZN2 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111468379 PE=3 SV=1)

HSP 1 Score: 984.9 bits (2545), Expect = 1.3e-283
Identity = 509/550 (92.55%), Postives = 526/550 (95.64%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60
           MLSTLQPPRCSLSRF LHLPSKP FFHSKQTP    +S LTDQLP+SN+SQ L SQILSI
Sbjct: 1   MLSTLQPPRCSLSRFPLHLPSKPFFFHSKQTPYPIANSALTDQLPSSNASQSLHSQILSI 60

Query: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120
           RHSLLSRQITATQLA+SYLNRLRALEPHL SFLHVSE+V SDAREID KI RNEE+GPLA
Sbjct: 61  RHSLLSRQITATQLAESYLNRLRALEPHLNSFLHVSESVLSDAREIDGKIQRNEEVGPLA 120

Query: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180
           GV V VKDNICTAGMPST GSRIL+GYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS
Sbjct: 121 GVFVGVKDNICTAGMPSTGGSRILEGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180

Query: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240
           TE SAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK
Sbjct: 181 TEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240

Query: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300
           PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDT DATSSKREV DF SQF
Sbjct: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTHDATSSKREVPDFASQF 300

Query: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360
           SAVDS ESKPL+GLRIGLIRETLD GVDG+VNSAIRAAASHLEELGCSINEVSLPSFSLG
Sbjct: 301 SAVDSLESKPLKGLRIGLIRETLDNGVDGEVNSAIRAAASHLEELGCSINEVSLPSFSLG 360

Query: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420
           LPAYYILASSE+SSNLARYDGVRYGNQA+ADELTGLYENSRATGFGSEVKMRILMGTYAL
Sbjct: 361 LPAYYILASSEASSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMGTYAL 420

Query: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480
           SAGYYDAYYKRAQQVRTIIQKSFR ALDE+DILISPAAPSAAYKIGEKV+DPLAMYAGDI
Sbjct: 421 SAGYYDAYYKRAQQVRTIIQKSFREALDENDILISPAAPSAAYKIGEKVNDPLAMYAGDI 480

Query: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540
           MTVNVNLAGLPALVLPCGFV++GSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTL++ RFV
Sbjct: 481 MTVNVNLAGLPALVLPCGFVRNGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLSERRFV 540

Query: 541 PPLLADDIVG 551
           PPLLADDIVG
Sbjct: 541 PPLLADDIVG 550

BLAST of CSPI06G22820 vs. ExPASy TrEMBL
Match: A0A6J1JPA2 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111486276 PE=3 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 2.3e-283
Identity = 509/554 (91.88%), Postives = 530/554 (95.67%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPH----STLTDQLPTSNSSQPLQSQ 60
           MLSTLQPPR SLSRF LHLPSKP FFHSKQTPLS+P+    +TLTDQ+ TS SS  L SQ
Sbjct: 1   MLSTLQPPRYSLSRFPLHLPSKPFFFHSKQTPLSSPYPIANATLTDQIATSASSPSLHSQ 60

Query: 61  ILSIRHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEEL 120
           ILSIRHSLLSRQ TATQLA+SYLNRLRALEPHLKSFLHVS++V SD REIDDKILRNEEL
Sbjct: 61  ILSIRHSLLSRQFTATQLAESYLNRLRALEPHLKSFLHVSDSVLSDGREIDDKILRNEEL 120

Query: 121 GPLAGVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFG 180
           GPLAGVLV VKDNICTAGMPST GSRILDGYRPPFDATAV+RIKELGGIV+GKTNLDEFG
Sbjct: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVRRIKELGGIVVGKTNLDEFG 180

Query: 181 MGSSTEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240
           MGS+TEASAFQVTANPWDL RVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV
Sbjct: 181 MGSTTEASAFQVTANPWDLKRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240

Query: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDF 300
           VGLKPTYGRVSRFGLMAYASSLDVIGCL TTVADAGILLHAISGHDTLDATSSKREV DF
Sbjct: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLHAISGHDTLDATSSKREVHDF 300

Query: 301 TSQFSAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPS 360
           TSQF+AVDS ESKPL+GLRIGLIRETLD GVDG+V+SAIRAAASHLEELGCSINEVSLPS
Sbjct: 301 TSQFTAVDSLESKPLKGLRIGLIRETLDNGVDGKVSSAIRAAASHLEELGCSINEVSLPS 360

Query: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMG 420
           FSLGLPAYYILASSESSSNLARYDGVRYGNQAIADELTGLY+NSRATGFGSEVKMRILMG
Sbjct: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYQNSRATGFGSEVKMRILMG 420

Query: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMY 480
           TYALSAGYYDAYYKRAQQVRTIIQKSF+AALDE+DILISPAAPSAAYKIGEKV+DPLAMY
Sbjct: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFKAALDEYDILISPAAPSAAYKIGEKVNDPLAMY 480

Query: 481 AGDIMTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLAD 540
           AGDIMTVNVNLAGLPALVLPCGFV+DGSS+LPVGLQMIGAAFDEGKL+KVGHIFEQTL D
Sbjct: 481 AGDIMTVNVNLAGLPALVLPCGFVRDGSSDLPVGLQMIGAAFDEGKLVKVGHIFEQTLPD 540

Query: 541 CRFVPPLLADDIVG 551
           CRFVPPLLAD IVG
Sbjct: 541 CRFVPPLLADHIVG 554

BLAST of CSPI06G22820 vs. NCBI nr
Match: XP_004140137.1 (glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial [Cucumis sativus])

HSP 1 Score: 1059.7 bits (2739), Expect = 8.7e-306
Identity = 547/550 (99.45%), Postives = 549/550 (99.82%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60
           MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI
Sbjct: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60

Query: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120
           RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA
Sbjct: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120

Query: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180
           GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS
Sbjct: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180

Query: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240
           TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK
Sbjct: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240

Query: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300
           PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLH+ISGHDTLDATSSKREVSDFTSQF
Sbjct: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHSISGHDTLDATSSKREVSDFTSQF 300

Query: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360
           SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG
Sbjct: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360

Query: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420
           LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL
Sbjct: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420

Query: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480
           SAGYYDAYYKRAQQVRTIIQKSFRAALDE+DILISP APSAAYKIGEKVDDPLAMYAGDI
Sbjct: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEYDILISPTAPSAAYKIGEKVDDPLAMYAGDI 480

Query: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540
           MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV
Sbjct: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540

Query: 541 PPLLADDIVG 551
           PPLLADDIVG
Sbjct: 541 PPLLADDIVG 550

BLAST of CSPI06G22820 vs. NCBI nr
Match: KGN48252.2 (hypothetical protein Csa_003783 [Cucumis sativus])

HSP 1 Score: 1056.6 bits (2731), Expect = 7.4e-305
Identity = 545/549 (99.27%), Postives = 548/549 (99.82%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60
           MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI
Sbjct: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60

Query: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120
           RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA
Sbjct: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120

Query: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180
           GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS
Sbjct: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180

Query: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240
           TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK
Sbjct: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240

Query: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300
           PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLH+ISGHDTLDATSSKREVSDFTSQF
Sbjct: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHSISGHDTLDATSSKREVSDFTSQF 300

Query: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360
           SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG
Sbjct: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360

Query: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420
           LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL
Sbjct: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420

Query: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480
           SAGYYDAYYKRAQQVRTIIQKSFRAALDE+DILISP APSAAYKIGEKVDDPLAMYAGDI
Sbjct: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEYDILISPTAPSAAYKIGEKVDDPLAMYAGDI 480

Query: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540
           MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV
Sbjct: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540

Query: 541 PPLLADDIV 550
           PPLLADDI+
Sbjct: 541 PPLLADDII 549

BLAST of CSPI06G22820 vs. NCBI nr
Match: XP_008449505.1 (PREDICTED: glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial [Cucumis melo])

HSP 1 Score: 1055.8 bits (2729), Expect = 1.3e-304
Identity = 545/550 (99.09%), Postives = 549/550 (99.82%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60
           MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI
Sbjct: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60

Query: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120
           RHSLLSRQITATQLADSYL+RLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA
Sbjct: 61  RHSLLSRQITATQLADSYLDRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120

Query: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180
           GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVK+IKELGGIVIGKTNLDEFGMGSS
Sbjct: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKKIKELGGIVIGKTNLDEFGMGSS 180

Query: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240
           TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK
Sbjct: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240

Query: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300
           PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF
Sbjct: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300

Query: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360
           SAVDSFESKPL+GLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG
Sbjct: 301 SAVDSFESKPLKGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360

Query: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420
           LPAYYILASSESSSNLARYDGVRYGNQA ADELTGLYENSRATGFGSEVKMRILMGTYAL
Sbjct: 361 LPAYYILASSESSSNLARYDGVRYGNQATADELTGLYENSRATGFGSEVKMRILMGTYAL 420

Query: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480
           SAGYYDAYYKRAQQVRTIIQKSFRAALDE+DILISPAAPSAAYKIGEKVDDPLAMYAGDI
Sbjct: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480

Query: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540
           MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV
Sbjct: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540

Query: 541 PPLLADDIVG 551
           PPLLADDIVG
Sbjct: 541 PPLLADDIVG 550

BLAST of CSPI06G22820 vs. NCBI nr
Match: XP_038888240.1 (glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 1008.8 bits (2607), Expect = 1.8e-290
Identity = 523/554 (94.40%), Postives = 534/554 (96.39%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLST----PHSTLTDQLPTSNSSQPLQSQ 60
           MLSTLQPPRCSLSRF LHLPSKP FFHSKQTPLS+    P+STLTDQLPTSNSSQ L SQ
Sbjct: 1   MLSTLQPPRCSLSRFPLHLPSKPFFFHSKQTPLSSSHSIPNSTLTDQLPTSNSSQSLHSQ 60

Query: 61  ILSIRHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEEL 120
           ILSIRHSLLSR ITATQLA+SYLNRLRALEPHLKSFLHVSETV SDAREIDDKI RNEEL
Sbjct: 61  ILSIRHSLLSRHITATQLAESYLNRLRALEPHLKSFLHVSETVLSDAREIDDKIHRNEEL 120

Query: 121 GPLAGVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFG 180
           GPLAGVLV VKDNICT+GMPST GSRILDGYRPPFDATAVKRIKELGGIV+GKTNLDEFG
Sbjct: 121 GPLAGVLVGVKDNICTSGMPSTGGSRILDGYRPPFDATAVKRIKELGGIVVGKTNLDEFG 180

Query: 181 MGSSTEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240
           MGSSTE SAFQVTANPWDL+RVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV
Sbjct: 181 MGSSTEGSAFQVTANPWDLNRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240

Query: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDF 300
           VGLKPTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAI GHDTLDATSSKREV D 
Sbjct: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAICGHDTLDATSSKREVPDL 300

Query: 301 TSQFSAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPS 360
           TSQFSAVDS ESKPL+GLRIGLIRETLD GVDGQVNSAIRAAASHLEELGCSINEVSLPS
Sbjct: 301 TSQFSAVDSLESKPLKGLRIGLIRETLDNGVDGQVNSAIRAAASHLEELGCSINEVSLPS 360

Query: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMG 420
           FSLGLPAYYILASSESSSNLARYD VRYGNQA+ADELTGLYENSRATGFGSEVKMRILMG
Sbjct: 361 FSLGLPAYYILASSESSSNLARYDSVRYGNQAVADELTGLYENSRATGFGSEVKMRILMG 420

Query: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMY 480
           TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDE+DILISPAAP+AAYKIGEKVDDPLAMY
Sbjct: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPTAAYKIGEKVDDPLAMY 480

Query: 481 AGDIMTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLAD 540
           AGDIMTVNVNLAGLPALVLPCGFVQDGSS+LPVGLQMIGAAFDEGKLLKVGHIFEQTLAD
Sbjct: 481 AGDIMTVNVNLAGLPALVLPCGFVQDGSSSLPVGLQMIGAAFDEGKLLKVGHIFEQTLAD 540

Query: 541 CRFVPPLLADDIVG 551
           CRFVPPLLADDIVG
Sbjct: 541 CRFVPPLLADDIVG 554

BLAST of CSPI06G22820 vs. NCBI nr
Match: KAA0061669.1 (glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo var. makuwa] >TYK21144.1 glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo var. makuwa])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.4e-289
Identity = 524/550 (95.27%), Postives = 528/550 (96.00%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60
           MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI
Sbjct: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60

Query: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120
           RHSLLSRQITATQLADSYL+RLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA
Sbjct: 61  RHSLLSRQITATQLADSYLDRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120

Query: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180
           GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVK+IKELGGIVIGKTNLDEFGMGSS
Sbjct: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKKIKELGGIVIGKTNLDEFGMGSS 180

Query: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240
           TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK
Sbjct: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240

Query: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300
           PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF
Sbjct: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300

Query: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360
           SAVDSFESKPL+GLRIGLIRETLDKGVDGQ                     VSLPSFSLG
Sbjct: 301 SAVDSFESKPLKGLRIGLIRETLDKGVDGQ---------------------VSLPSFSLG 360

Query: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420
           LPAYYILASSESSSNLARYDGVRYGNQA ADELTGLYENSRATGFGSEVKMRILMGTYAL
Sbjct: 361 LPAYYILASSESSSNLARYDGVRYGNQATADELTGLYENSRATGFGSEVKMRILMGTYAL 420

Query: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480
           SAGYYDAYYKRAQQVRTIIQKSFRAALDE+DILISPAAPSAAYKIGEKVDDPLAMYAGDI
Sbjct: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480

Query: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540
           MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV
Sbjct: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 529

Query: 541 PPLLADDIVG 551
           PPLLADDIVG
Sbjct: 541 PPLLADDIVG 529

BLAST of CSPI06G22820 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 781.6 bits (2017), Expect = 4.3e-226
Identity = 412/546 (75.46%), Postives = 461/546 (84.43%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPHSTLTDQLPTSNSSQPLQSQILSI 60
           MLSTLQPPR SLS   L        F   +T +S   S   D   TS  S P QSQIL+ 
Sbjct: 1   MLSTLQPPR-SLSLLPLRR------FQISKTIVSAASSKTID---TSVISPP-QSQILTT 60

Query: 61  RHSLLSRQITATQLADSYLNRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEELGPLA 120
           R SLLS + TA ++A SYL+R+R  EP LK FLHVSE V  DA+EID +I + EELGPLA
Sbjct: 61  RRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEIDQRIAKGEELGPLA 120

Query: 121 GVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVIGKTNLDEFGMGSS 180
           GVL+ VKDNICT GMPSTA SRIL+ YRPPFDATAVK+IKELGGIV+GKTN+DEFGMGS+
Sbjct: 121 GVLIGVKDNICTQGMPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEFGMGST 180

Query: 181 TEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLK 240
           TEASAFQVTANPWDLSRVPGGSSGGSA+AV+ARQC+VSLGSDTGGSVRQPASFCGVVGLK
Sbjct: 181 TEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLK 240

Query: 241 PTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDFTSQF 300
           PTYGRVSRFGLMAYASSLDVIGC  +TVADAG+LLHAISG+D  D+TSSK++V +F SQF
Sbjct: 241 PTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSSKQDVPEFQSQF 300

Query: 301 SAVDSFESKPLRGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPSFSLG 360
            +VD FESKPL G+++G+IRETL+ GVD  V SA + AASHLE LGC + EVSLPSFSLG
Sbjct: 301 LSVDHFESKPLNGVKVGIIRETLEDGVDSGVRSATQEAASHLEALGCILTEVSLPSFSLG 360

Query: 361 LPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYENSRATGFGSEVKMRILMGTYAL 420
           LPAYY++ASSESSSNL+RYDGVRYGNQ +A+EL  LYE SR  GFG EVKMRILMGTYAL
Sbjct: 361 LPAYYVIASSESSSNLSRYDGVRYGNQVMAEELNKLYECSRGEGFGGEVKMRILMGTYAL 420

Query: 421 SAGYYDAYYKRAQQVRTIIQKSFRAALDEHDILISPAAPSAAYKIGEKVDDPLAMYAGDI 480
           SAGYYDAYYKRAQQVRT+I+K F+AAL+++DILISPAAPSAAYKIGEK DDPLAMYAGDI
Sbjct: 421 SAGYYDAYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAGDI 480

Query: 481 MTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLADCRFV 540
           MTVNVNLAGLPA+VLPCG V+ G S LPVGLQMIGAAFDE KLLKVGHIFEQTL    FV
Sbjct: 481 MTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLKGSSFV 535

Query: 541 PPLLAD 547
           PPLLA+
Sbjct: 541 PPLLAN 535

BLAST of CSPI06G22820 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 144.8 bits (364), Expect = 2.0e-34
Identity = 122/452 (26.99%), Postives = 204/452 (45.13%), Query Frame = 0

Query: 99  VRSDAREI----DDKILRNEELGPLA---GVLVAVKDNICTAGMPSTAGSRILDGYRP-P 158
           +R DA E+    +    R E+  P++   G+ V +KD+I     P+  G+  L   R   
Sbjct: 170 IRFDANEVIKQAEASTRRFEQGNPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVE 229

Query: 159 FDATAVKRIKELGGIVIGKTNLDEFGMGSSTEASAFQVTANPWDLSRVPGGSSGGSASAV 218
            D+  V +++  G I++GK N+ E GMG++   S +  T NP D  R  GGSS GSA+ V
Sbjct: 230 KDSAVVSKLRSCGAILLGKANMHELGMGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIV 289

Query: 219 SARQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCLSTTVAD 278
           +A  C  +LG+D GGSVR P++ CG+ GLK TYGR    G +    ++++IG L++++ D
Sbjct: 290 AAGLCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLED 349

Query: 279 AGILLHAISGHDTLDATSSKREVSDFTSQFSAVDSFESKPLRGLRIGLIRETLDKGVDGQ 338
           A ++  AI G  + D  + K     F    S      S  +  LR+G   +  +      
Sbjct: 350 AFLVYAAILGSSSADRYNLKPSPPCFPKLLS---HNGSNAIGSLRLGKYTKWFNDVSSSD 409

Query: 339 VNSAIRAAASHL-EELGCSINEVSLPSFSLGLPAYYILASSESSSNLARYDGVRYGNQAI 398
           ++         L    GC + E+ +P       A+ I   S + S+L  Y      ++  
Sbjct: 410 ISDKCEDILKLLSNNHGCKVVEIVVPELEEMRAAHVISIGSPTLSSLTPYCEAGKNSKLS 469

Query: 399 ADELTGLYENSRATGFGSEVKMRILMGTYALSAGYYDAYYKRAQQVRTIIQKSFRAALDE 458
            D  T                      ++A+   +  + Y  AQ +R  + +       +
Sbjct: 470 YDTRT----------------------SFAIFRSFSASDYIAAQCLRRRLMEYHLNIFKD 529

Query: 459 HDILISPAA-------PSAAYKIGEKVDDPLAMYAGDIM--TVNVNLAGLPALVLPCGFV 518
            D++++P         P  A K GE           D+M   +  NL G PA+ +P G+ 
Sbjct: 530 VDVIVTPTTGMTAPVIPPDALKNGE----TNIQVTTDLMRFVLAANLLGFPAISVPVGYD 589

Query: 519 QDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQ 533
           ++G   LP+GLQ++G  + E  +L +    E+
Sbjct: 590 KEG---LPIGLQIMGRPWAEATVLGLAAAVEE 589

BLAST of CSPI06G22820 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 124.0 bits (310), Expect = 3.7e-28
Identity = 81/251 (32.27%), Postives = 127/251 (50.60%), Query Frame = 0

Query: 57  ILSIRHSLLSRQITATQLADSYLNRLRALEPHLKSFL-HVSETVRSDAREIDDKILRNEE 116
           +L +   + +RQIT+ +L   YL +L+     L++ + +  E     A+E DD + +   
Sbjct: 205 VLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTY 264

Query: 117 LGPLAGVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVI-----GKT 176
           LGPL G+   +KD +   G  +T GS          +A   KR+K  G +++     G  
Sbjct: 265 LGPLHGIPYGLKDIVAVPGYKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSM 324

Query: 177 NLDEFGMGSSTEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQP 236
             D+   G  T         NPW++     GSS G A++ SA     ++GS+T GS+  P
Sbjct: 325 AYDDIWFGGRTR--------NPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYP 384

Query: 237 ASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSK 296
           A+ CG+  L+PT+G V R G+M+ + SLD +G    T AD  ++L AI G D  D +S  
Sbjct: 385 AARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSS-- 444

Query: 297 REVSDFTSQFS 302
           RE++ F   FS
Sbjct: 445 REIA-FEDPFS 444

BLAST of CSPI06G22820 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 117.9 bits (294), Expect = 2.7e-26
Identity = 81/251 (32.27%), Postives = 125/251 (49.80%), Query Frame = 0

Query: 57  ILSIRHSLLSRQITATQLADSYLNRLRALEPHLKSFL-HVSETVRSDAREIDDKILRNEE 116
           +L +   + +RQIT+ +L   YL +L+     L++ + +  E     A+E DD + +   
Sbjct: 205 VLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTY 264

Query: 117 LGPLAGVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKRIKELGGIVI-----GKT 176
           LGPL G+   +KD +   G  +T GS          +A   KR+K  G +++     G  
Sbjct: 265 LGPLHGIPYGLKDIVAVPGYKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSM 324

Query: 177 NLDEFGMGSSTEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQP 236
             D+   G  T         NPW++     GSS G A++ SA       GS+T GS+  P
Sbjct: 325 AYDDIWFGGRTR--------NPWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYP 384

Query: 237 ASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSK 296
           A+ CG+  L+PT+G V R G+M+ + SLD +G    T AD  ++L AI G D  D +S  
Sbjct: 385 AARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSS-- 437

Query: 297 REVSDFTSQFS 302
           RE++ F   FS
Sbjct: 445 REIA-FEDPFS 437

BLAST of CSPI06G22820 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 96.3 bits (238), Expect = 8.3e-20
Identity = 61/155 (39.35%), Postives = 82/155 (52.90%), Query Frame = 0

Query: 119 LAGVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATA----VKRIKELGGIVIGKTNLDE 178
           L G+  A+KD     G  +  G+   D  R    AT+    V  + E G   +G T +DE
Sbjct: 28  LQGLTFAIKDIFDVEGRVTGFGNP--DWLRTHSAATSTAPVVSSLLEAGATALGITIMDE 87

Query: 179 FGMGSSTEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFC 238
                + E + +    NP    RVPGGSS GSA AV+AR    S+G+DTGGSVR PAS+C
Sbjct: 88  MAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLVDFSIGTDTGGSVRVPASYC 147

Query: 239 GVVGLKPTYGRVSRFGLMAYASSLDVIGCLSTTVA 270
           G+ G +P++G VS  GL   A S D +G  +   A
Sbjct: 148 GIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTA 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
E0CTY11.6e-22575.45Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Vi... [more]
Q9LI776.0e-22575.46Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Ar... [more]
B6U1515.1e-20066.79Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Ze... [more]
C5Y8Z81.5e-19966.97Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=So... [more]
Q25A685.7e-19966.30Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Or... [more]
Match NameE-valueIdentityDescription
A0A0A0KEN84.2e-30699.45Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cu... [more]
A0A1S3BM646.1e-30599.09Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cu... [more]
A0A5D3DC363.6e-28995.27Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cu... [more]
A0A6J1HZN21.3e-28392.55Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cu... [more]
A0A6J1JPA22.3e-28391.88Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cu... [more]
Match NameE-valueIdentityDescription
XP_004140137.18.7e-30699.45glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial [Cucu... [more]
KGN48252.27.4e-30599.27hypothetical protein Csa_003783 [Cucumis sativus][more]
XP_008449505.11.3e-30499.09PREDICTED: glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochon... [more]
XP_038888240.11.8e-29094.40glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial [Beni... [more]
KAA0061669.17.4e-28995.27glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo var. makuwa] >TYK211... [more]
Match NameE-valueIdentityDescription
AT3G25660.14.3e-22675.46Amidase family protein [more]
AT5G64440.12.0e-3426.99fatty acid amide hydrolase [more]
AT5G07360.13.7e-2832.27Amidase family protein [more]
AT5G07360.22.7e-2632.27Amidase family protein [more]
AT1G08980.18.3e-2039.35amidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 52..538
e-value: 5.1E-170
score: 568.5
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 55..541
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 75..524
e-value: 6.2E-144
score: 480.3
IPR004412Glutamyl-tRNA(Gln) amidotransferase A subunitTIGRFAMTIGR00132TIGR00132coord: 62..534
e-value: 1.7E-174
score: 579.0
IPR004412Glutamyl-tRNA(Gln) amidotransferase A subunitHAMAPMF_00120GatAcoord: 60..535
score: 88.690704
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..52
NoneNo IPR availablePANTHERPTHR11895:SF7GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIALcoord: 54..531
IPR000120AmidasePANTHERPTHR11895TRANSAMIDASEcoord: 54..531
IPR020556Amidase, conserved sitePROSITEPS00571AMIDASEScoord: 200..231

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G22820.1CSPI06G22820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070681 glutaminyl-tRNAGln biosynthesis via transamidation
biological_process GO:0032543 mitochondrial translation
biological_process GO:0006412 translation
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0030956 glutamyl-tRNA(Gln) amidotransferase complex
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0050567 glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
molecular_function GO:0016740 transferase activity
molecular_function GO:0003824 catalytic activity