CSPI06G22650 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G22650
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description1-aminocyclopropane-1-carboxylate oxidase 1
LocationChr6: 20476809 .. 20479234 (+)
RNA-Seq ExpressionCSPI06G22650
SyntenyCSPI06G22650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTCTTTCCATATGTATTGAATCAAAGAGAGGAAGGGGAAGTAGGGGATATGGCATTGGCAGACATAAAAATAATAAAGTTAAATCAAATTGGGTCCCTCAGACTCACCAAAGAGGAAATTCAGTGTTGAATAAAGCCAAAGCCAAAGCCAAAGCCAAAGCCAAAGCCACCTTCCCCCTCTTTCCCACATACATGCATGAAATTTTAAGGGCCCATTCTTTTTATCATCACATTTTTAATAACTTATCTTCTCTCTTCTTCTTCTTCTTCTGCTCTTTTCTAATCAATTTCTTCCCACTTTCCAATCCTAAGTAATTTCACTACTATAAATACCCCTTCATTATAACTTGATCCAACACACACCCACCAAACAAAAACAAAACCTCGATACCATAGAGAGTTTCTTTTTTACCTTTAGTTGGGCAAACCAAAATTTGTATCTACAAAAAAGAATGGCTGTCTTTCCTATCATCAACTTGGAAAACATCAACGGTGATGGTAGAGCTAAGATATTGGAGCAAATTGAAGATGCCTGCCAAAATTGGGGTTTCTTTGAGGTGATTTCATAGATTAATTATCCTCTCTCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTCTCTCTTTGAGGTGATGTCCTGATTTGATTTTAATATGCAGTTGGTGAACCATGGGATCCCACATGAGTTTTTGGACATGGTTGAGAAGATGACAAGAGATCATGACAAGAAATGTATGGAAGAGAGGTTTAAGGAGACTGTTCTTAGCAAAGGCTTAGAGGCTGCACAAGCTGAAGTTAATGATATGGATTGGGAAAGCACCTTCTTCTTACGCCATCTTCCCGAATCAAACATCTCCCAGATGTCTGATCTCGACGAGGAGTATAAGTATGATATTATCTAATTTAAATTGTATATAAATGTGTGTATAATATTAATCTTTTAGGACATTACTATTCGGATTCTCAACAATTTTTAATCATATGTGAATGATTGGCAGGAAAATTATGAAGGAATTTGCGAAGAAATTGGAGAATCTTGCTGAGGAGTTGTTGGACCTGCTATGTGAGAATCTTGGGTTGGAAAAGGGTTATCTCAAAAATGCTTTCTATGGTTCGAAAGGTCCTACATTTGGAACAAAGGTTAGCAAATATCCACCGTGTCCCAAGCCCGACCTCATCAAGGGTCTTCGAGCCCACACCGACGCCGGTGGCATCATCCTCCTCTTCCAAGATGACAAGGTAGGTGGCCTGCAACTCCTGAAAGATGGCGACTGGATCGATGTGCCCCCAATGCGCCATGCCATTGTCGTCAACCTCGGAGACCAACTTGAGGTAATATTATATACTAATACTTATAAAGCAAAGTAGAAAAGAAGGACAACGATTAAATGATCTATTATCTGCGTTGCATAAAAGATTAGATGGAACATAAGACAATATCCACATAAGTTTTATTTTCTATTAACGTAAAACTTGGCAGGTGATCACAAATGGAAGATACAAAAGTGTGATGCACAGAGTGTTAATTACTCAAACAAGTGGAACTGGACGAATGTCGATAGCTTCATTCTACAATCCGGGGAGCGACGCGGTGATCTACCCTGCGCCGGCGCTAGTAGAGAAAGATGAGGAGAAGAAGGAAGTGTACCCCAAGTTTGTGTTTGAAGATTACATGAAGCTGTACCTAGGAGTAAAGTTTCAGGCTAAGGAGCCAAGATTTGAAGCCATGAAAGCCAATGCTAATTTGGGTCCAATGGCAACAGCATAAAACGCCCACTTTTTAATCAATTATTAGTAATAAAGGAATGTTAGAGGGCTTGTCTTTGTGTTTGCCCTTTTTAAGTGGGTCATTATGTGTTATTAAATTTAATTAAGTGGGTCATTATTGTTATTAAATTTAGTTGAAAAGTTAAAACCAAATATATATATATATATATATTATTTATGTTTGTGTTGTTCCC

mRNA sequence

GTTTTCTTTCCATATGTATTGAATCAAAGAGAGGAAGGGGAAGTAGGGGATATGGCATTGGCAGACATAAAAATAATAAAGTTAAATCAAATTGGGTCCCTCAGACTCACCAAAGAGGAAATTCAGTGTTGAATAAAGCCAAAGCCAAAGCCAAAGCCAAAGCCAAAGCCACCTTCCCCCTCTTTCCCACATACATGCATGAAATTTTAAGGGCCCATTCTTTTTATCATCACATTTTTAATAACTTATCTTCTCTCTTCTTCTTCTTCTTCTGCTCTTTTCTAATCAATTTCTTCCCACTTTCCAATCCTAAGTAATTTCACTACTATAAATACCCCTTCATTATAACTTGATCCAACACACACCCACCAAACAAAAACAAAACCTCGATACCATAGAGAGTTTCTTTTTTACCTTTAGTTGGGCAAACCAAAATTTGTATCTACAAAAAAGAATGGCTGTCTTTCCTATCATCAACTTGGAAAACATCAACGGTGATGGTAGAGCTAAGATATTGGAGCAAATTGAAGATGCCTGCCAAAATTGGGGTTTCTTTGAGTTGGTGAACCATGGGATCCCACATGAGTTTTTGGACATGGTTGAGAAGATGACAAGAGATCATGACAAGAAATGTATGGAAGAGAGGTTTAAGGAGACTGTTCTTAGCAAAGGCTTAGAGGCTGCACAAGCTGAAGTTAATGATATGGATTGGGAAAGCACCTTCTTCTTACGCCATCTTCCCGAATCAAACATCTCCCAGATGTCTGATCTCGACGAGGAGTATAAGAAAATTATGAAGGAATTTGCGAAGAAATTGGAGAATCTTGCTGAGGAGTTGTTGGACCTGCTATGTGAGAATCTTGGGTTGGAAAAGGGTTATCTCAAAAATGCTTTCTATGGTTCGAAAGGTCCTACATTTGGAACAAAGGTTAGCAAATATCCACCGTGTCCCAAGCCCGACCTCATCAAGGGTCTTCGAGCCCACACCGACGCCGGTGGCATCATCCTCCTCTTCCAAGATGACAAGGTAGGTGGCCTGCAACTCCTGAAAGATGGCGACTGGATCGATGTGCCCCCAATGCGCCATGCCATTGTCGTCAACCTCGGAGACCAACTTGAGGTGATCACAAATGGAAGATACAAAAGTGTGATGCACAGAGTGTTAATTACTCAAACAAGTGGAACTGGACGAATGTCGATAGCTTCATTCTACAATCCGGGGAGCGACGCGGTGATCTACCCTGCGCCGGCGCTAGTAGAGAAAGATGAGGAGAAGAAGGAAGTGTACCCCAAGTTTGTGTTTGAAGATTACATGAAGCTGTACCTAGGAGTAAAGTTTCAGGCTAAGGAGCCAAGATTTGAAGCCATGAAAGCCAATGCTAATTTGGGTCCAATGGCAACAGCATAAAACGCCCACTTTTTAATCAATTATTAGTAATAAAGGAATGTTAGAGGGCTTGTCTTTGTGTTTGCCCTTTTTAAGTGGGTCATTATGTGTTATTAAATTTAATTAAGTGGGTCATTATTGTTATTAAATTTAGTTGAAAAGTTAAAACCAAATATATATATATATATATATTATTTATGTTTGTGTTGTTCCC

Coding sequence (CDS)

ATGGCTGTCTTTCCTATCATCAACTTGGAAAACATCAACGGTGATGGTAGAGCTAAGATATTGGAGCAAATTGAAGATGCCTGCCAAAATTGGGGTTTCTTTGAGTTGGTGAACCATGGGATCCCACATGAGTTTTTGGACATGGTTGAGAAGATGACAAGAGATCATGACAAGAAATGTATGGAAGAGAGGTTTAAGGAGACTGTTCTTAGCAAAGGCTTAGAGGCTGCACAAGCTGAAGTTAATGATATGGATTGGGAAAGCACCTTCTTCTTACGCCATCTTCCCGAATCAAACATCTCCCAGATGTCTGATCTCGACGAGGAGTATAAGAAAATTATGAAGGAATTTGCGAAGAAATTGGAGAATCTTGCTGAGGAGTTGTTGGACCTGCTATGTGAGAATCTTGGGTTGGAAAAGGGTTATCTCAAAAATGCTTTCTATGGTTCGAAAGGTCCTACATTTGGAACAAAGGTTAGCAAATATCCACCGTGTCCCAAGCCCGACCTCATCAAGGGTCTTCGAGCCCACACCGACGCCGGTGGCATCATCCTCCTCTTCCAAGATGACAAGGTAGGTGGCCTGCAACTCCTGAAAGATGGCGACTGGATCGATGTGCCCCCAATGCGCCATGCCATTGTCGTCAACCTCGGAGACCAACTTGAGGTGATCACAAATGGAAGATACAAAAGTGTGATGCACAGAGTGTTAATTACTCAAACAAGTGGAACTGGACGAATGTCGATAGCTTCATTCTACAATCCGGGGAGCGACGCGGTGATCTACCCTGCGCCGGCGCTAGTAGAGAAAGATGAGGAGAAGAAGGAAGTGTACCCCAAGTTTGTGTTTGAAGATTACATGAAGCTGTACCTAGGAGTAAAGTTTCAGGCTAAGGAGCCAAGATTTGAAGCCATGAAAGCCAATGCTAATTTGGGTCCAATGGCAACAGCATAA

Protein sequence

MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKCMEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKKLENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDAGGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQTSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEPRFEAMKANANLGPMATA*
Homology
BLAST of CSPI06G22650 vs. ExPASy Swiss-Prot
Match: Q04644 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=ACO1 PE=2 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 2.1e-177
Identity = 310/319 (97.18%), Postives = 311/319 (97.49%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           MAVFPIINLENIN DGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDH KKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LENLAEELLDLLCENLGLEKGYLK AFYGSKGPTFGTKVS YPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKV GLQLLKDG+WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL TQ
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL-TQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEK--DEEKKEVYPKFVFEDYMKLYLGVKFQAK 300
           TSGTGRMSIASFYNPGSDAVIYPAPALVEK  DEEKKEVYPKFVFEDYMKLYLGVKFQAK
Sbjct: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAK 300

Query: 301 EPRFEAMKANANLGPMATA 318
           EPRFEAMKANANLGPMATA
Sbjct: 301 EPRFEAMKANANLGPMATA 318

BLAST of CSPI06G22650 vs. ExPASy Swiss-Prot
Match: P05116 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Solanum lycopersicum OX=4081 GN=ACO1 PE=2 SV=2)

HSP 1 Score: 518.8 bits (1335), Expect = 4.2e-146
Identity = 255/318 (80.19%), Postives = 281/318 (88.36%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           M  FPIINLE +NGD RA  +E I+DAC+NWGFFELVNHGIPHE +D VEKMT+ H KKC
Sbjct: 1   MENFPIINLEKLNGDERANTMEMIKDACENWGFFELVNHGIPHEVMDTVEKMTKGHYKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           ME+RFKE V SKGLEA QAEV D+DWESTFFLRHLP SNISQ+ DLDEEY+++M++FAK+
Sbjct: 61  MEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPTSNISQVPDLDEEYREVMRDFAKR 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LE LAEELLDLLCENLGLEKGYLKNAFYGSKGP FGTKVS YPPCPKPDLIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKV GLQLLKD  WIDVPPMRH+IVVNLGDQLEVITNG+YKSV+HRV I Q
Sbjct: 181 GGIILLFQDDKVSGLQLLKDEQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRV-IAQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKD-EEKKEVYPKFVFEDYMKLYLGVKFQAKE 300
           T GT RMS+ASFYNPGSDAVIYPA  LVEK+ EE  +VYPKFVF+DYMKLY G+KFQAKE
Sbjct: 241 TDGT-RMSLASFYNPGSDAVIYPAKTLVEKEAEESTQVYPKFVFDDYMKLYAGLKFQAKE 300

Query: 301 PRFEAMKANANLGPMATA 318
           PRFEAMKA  +  P+A+A
Sbjct: 301 PRFEAMKAMES-DPIASA 315

BLAST of CSPI06G22650 vs. ExPASy Swiss-Prot
Match: P24157 (1-aminocyclopropane-1-carboxylate oxidase 4 OS=Solanum lycopersicum OX=4081 GN=ACO4 PE=2 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 4.2e-146
Identity = 251/307 (81.76%), Postives = 276/307 (89.90%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           M  FPIINLEN+NGD RAK +E I+DAC+NWGFFELVNHGIPHE +D VEK+T+ H KKC
Sbjct: 1   MENFPIINLENLNGDERAKTMEMIKDACENWGFFELVNHGIPHEVMDTVEKLTKGHYKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           ME+RFKE V SKGLEA QAEV D+DWESTFFLRHLP SNISQ+ DLDEEY+++M++FAK+
Sbjct: 61  MEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPTSNISQVPDLDEEYREVMRDFAKR 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LE LAEELLDLLCENLGLEKGYLKNAFYGSKGP FGTKVS YPPCPKPDLIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKV GLQLLKD  WIDVPPMRH+IVVNLGDQLEVITNG+YKSVMHRV I Q
Sbjct: 181 GGIILLFQDDKVSGLQLLKDEQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVMHRV-IAQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP 300
           T GT RMS+ASFYNPG+DAVIYPAP+L+   EE K+VYPKFVF+DYMKLY G+KFQ KEP
Sbjct: 241 TDGT-RMSLASFYNPGNDAVIYPAPSLI---EESKQVYPKFVFDDYMKLYAGLKFQPKEP 300

Query: 301 RFEAMKA 308
           RFEAMKA
Sbjct: 301 RFEAMKA 302

BLAST of CSPI06G22650 vs. ExPASy Swiss-Prot
Match: Q08507 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 2.7e-145
Identity = 253/322 (78.57%), Postives = 281/322 (87.27%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           M  FPIINLE +NG  R   +E I+DAC+NWGFFELVNHGIPHE +D VEK+T+ H KKC
Sbjct: 1   MENFPIINLEKLNGSERDATMEMIKDACENWGFFELVNHGIPHEVMDTVEKLTKGHYKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           ME+RFKE V SKGLEA QAEV D+DWESTFFLRHLP SNIS++ DLD+EY+++M++FAK+
Sbjct: 61  MEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNISEVPDLDDEYREVMRDFAKR 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LE LAEELLDLLCENLGLEKGYLK AFYGSKGP FGTKVS YPPCPKPDLIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKV GLQLLKDG WIDVPPMRH+IVVNLGDQLEVITNG+YKSV+HRV I Q
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRV-IAQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEK--DEEKKEVYPKFVFEDYMKLYLGVKFQAK 300
           T GT RMS+ASFYNPGSDAVIYPAP LVEK  D+E K+VYPKFVF+DYMKLY G+KFQAK
Sbjct: 241 TDGT-RMSLASFYNPGSDAVIYPAPTLVEKEADQECKQVYPKFVFDDYMKLYAGLKFQAK 300

Query: 301 EPRFEAMK---ANANLGPMATA 318
           EPRFEAMK   A+    P+ATA
Sbjct: 301 EPRFEAMKAREADVKSDPIATA 320

BLAST of CSPI06G22650 vs. ExPASy Swiss-Prot
Match: Q08506 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 PE=1 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 6.7e-144
Identity = 251/320 (78.44%), Postives = 279/320 (87.19%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           M  FPII+L+ +NG  RA  +E I+DAC+NWGFFELVNHGIP E +D VEKMT+ H KKC
Sbjct: 1   MENFPIISLDKVNGVERAATMEMIKDACENWGFFELVNHGIPREVMDTVEKMTKGHYKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           ME+RFKE V SK LE  QAEV DMDWESTFFL+HLP SNIS++ DLDEEY+++M++FAK+
Sbjct: 61  MEQRFKELVASKALEGVQAEVTDMDWESTFFLKHLPISNISEVPDLDEEYREVMRDFAKR 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LE LAEELLDLLCENLGLEKGYLKNAFYGSKGP FGTKVS YPPCPKPDLIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKV GLQLLKDG WIDVPPMRH+IVVNLGDQLEVITNG+YKSVMHRV I Q
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVMHRV-IAQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKD-EEKKEVYPKFVFEDYMKLYLGVKFQAKE 300
             G  RMS+ASFYNPGSDAVIYPAPALVEK+ EE K+VYPKFVF+DYMKLY G+KFQAKE
Sbjct: 241 KDG-ARMSLASFYNPGSDAVIYPAPALVEKEAEENKQVYPKFVFDDYMKLYAGLKFQAKE 300

Query: 301 PRFEAMKA---NANLGPMAT 317
           PRFEAMKA   +  + P+AT
Sbjct: 301 PRFEAMKAMETDVKMDPIAT 318

BLAST of CSPI06G22650 vs. ExPASy TrEMBL
Match: O82126 (ACC oxidase OS=Cucumis sativus OX=3659 GN=CS-ACO1 PE=2 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.3e-182
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC
Sbjct: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ
Sbjct: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP 300
           TSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP
Sbjct: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP 300

Query: 301 RFEAMKANANLGPMATA 318
           RFEAMKANANLGPMATA
Sbjct: 301 RFEAMKANANLGPMATA 317

BLAST of CSPI06G22650 vs. ExPASy TrEMBL
Match: O64947 (ACC oxidase 3 OS=Cucumis sativus OX=3659 GN=Cs-ACO3 PE=2 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 4.9e-182
Identity = 316/317 (99.68%), Postives = 316/317 (99.68%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC
Sbjct: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ
Sbjct: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP 300
            SGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP
Sbjct: 241 NSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP 300

Query: 301 RFEAMKANANLGPMATA 318
           RFEAMKANANLGPMATA
Sbjct: 301 RFEAMKANANLGPMATA 317

BLAST of CSPI06G22650 vs. ExPASy TrEMBL
Match: A0A5D3C3Q0 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1465G00060 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.2e-175
Identity = 311/319 (97.49%), Postives = 312/319 (97.81%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDH KKC
Sbjct: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LENLAEELLDLLCENLGLEKGYLK AFYGSKGPTFGTKVS YPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKV GLQLLKDG+WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL TQ
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL-TQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEK--DEEKKEVYPKFVFEDYMKLYLGVKFQAK 300
           TSGTGRMSIASFYNPGSDAVIYPAPALVEK  DEEKKEVYPKFVFEDYMKLYLGVKFQAK
Sbjct: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAK 300

Query: 301 EPRFEAMKANANLGPMATA 318
           EPRFEAMKANANLGPMATA
Sbjct: 301 EPRFEAMKANANLGPMATA 318

BLAST of CSPI06G22650 vs. ExPASy TrEMBL
Match: D0V4H6 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=LOC103491357 PE=2 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 7.6e-175
Identity = 310/319 (97.18%), Postives = 311/319 (97.49%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           MAVFPIINLENIN DGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDH KKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LENLAEELLDLLCENLGLEKGYLK AFYGSKGPTFGTKVS YPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKV GLQLLKDG+WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL TQ
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL-TQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEK--DEEKKEVYPKFVFEDYMKLYLGVKFQAK 300
           TSGTGRMSIASFYNPGSDAVIYPAPALVEK  DEEKKEVYPKFVFEDYMKLYLGVKFQAK
Sbjct: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAK 300

Query: 301 EPRFEAMKANANLGPMATA 318
           EPRFEAMKANANLGPMATA
Sbjct: 301 EPRFEAMKANANLGPMATA 318

BLAST of CSPI06G22650 vs. ExPASy TrEMBL
Match: C8KIS1 (ACC oxidase OS=Cucumis melo OX=3656 GN=CM-ACO1 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.7e-174
Identity = 309/319 (96.87%), Postives = 311/319 (97.49%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           MAVFPIINLENIN DGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDH KKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LENLAEELLDLLCENLGLEKGYLK AFYGSKGPTFGTKVS YPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKV GLQLLKDG+WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL TQ
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL-TQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEK--DEEKKEVYPKFVFEDYMKLYLGVKFQAK 300
           TSGTGRMSIASFYNPGSDAVIYPAPA+VEK  DEEKKEVYPKFVFEDYMKLYLGVKFQAK
Sbjct: 241 TSGTGRMSIASFYNPGSDAVIYPAPAVVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAK 300

Query: 301 EPRFEAMKANANLGPMATA 318
           EPRFEAMKANANLGPMATA
Sbjct: 301 EPRFEAMKANANLGPMATA 318

BLAST of CSPI06G22650 vs. NCBI nr
Match: NP_001267694.1 (1-aminocyclopropane-1-carboxylate oxidase 1-like [Cucumis sativus] >KAE8647342.1 hypothetical protein Csa_004013 [Cucumis sativus] >BAA33377.1 ACC oxidase [Cucumis sativus])

HSP 1 Score: 648.7 bits (1672), Expect = 2.7e-182
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC
Sbjct: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ
Sbjct: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP 300
           TSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP
Sbjct: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP 300

Query: 301 RFEAMKANANLGPMATA 318
           RFEAMKANANLGPMATA
Sbjct: 301 RFEAMKANANLGPMATA 317

BLAST of CSPI06G22650 vs. NCBI nr
Match: AAC67234.1 (ACC oxidase 3 [Cucumis sativus])

HSP 1 Score: 646.7 bits (1667), Expect = 1.0e-181
Identity = 316/317 (99.68%), Postives = 316/317 (99.68%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC
Sbjct: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ
Sbjct: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP 300
            SGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP
Sbjct: 241 NSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEP 300

Query: 301 RFEAMKANANLGPMATA 318
           RFEAMKANANLGPMATA
Sbjct: 301 RFEAMKANANLGPMATA 317

BLAST of CSPI06G22650 vs. NCBI nr
Match: KAA0061654.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo var. makuwa] >TYK05852.1 1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 625.5 bits (1612), Expect = 2.4e-175
Identity = 311/319 (97.49%), Postives = 312/319 (97.81%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDH KKC
Sbjct: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LENLAEELLDLLCENLGLEKGYLK AFYGSKGPTFGTKVS YPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKV GLQLLKDG+WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL TQ
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL-TQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEK--DEEKKEVYPKFVFEDYMKLYLGVKFQAK 300
           TSGTGRMSIASFYNPGSDAVIYPAPALVEK  DEEKKEVYPKFVFEDYMKLYLGVKFQAK
Sbjct: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAK 300

Query: 301 EPRFEAMKANANLGPMATA 318
           EPRFEAMKANANLGPMATA
Sbjct: 301 EPRFEAMKANANLGPMATA 318

BLAST of CSPI06G22650 vs. NCBI nr
Match: NP_001284392.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo] >Q04644.1 RecName: Full=1-aminocyclopropane-1-carboxylate oxidase 1; Short=ACC oxidase 1; AltName: Full=Ethylene-forming enzyme; Short=EFE; AltName: Full=PMEL1 [Cucumis melo] >ACY08855.1 ACC oxidase [Cucumis melo] >CAA49553.1 enzyme-forming ethylene [Cucumis melo] >CAA64797.1 ACC oxidase [Cucumis melo] >BAA06526.1 1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo])

HSP 1 Score: 622.9 bits (1605), Expect = 1.6e-174
Identity = 310/319 (97.18%), Postives = 311/319 (97.49%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           MAVFPIINLENIN DGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDH KKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LENLAEELLDLLCENLGLEKGYLK AFYGSKGPTFGTKVS YPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKV GLQLLKDG+WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL TQ
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL-TQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEK--DEEKKEVYPKFVFEDYMKLYLGVKFQAK 300
           TSGTGRMSIASFYNPGSDAVIYPAPALVEK  DEEKKEVYPKFVFEDYMKLYLGVKFQAK
Sbjct: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAK 300

Query: 301 EPRFEAMKANANLGPMATA 318
           EPRFEAMKANANLGPMATA
Sbjct: 301 EPRFEAMKANANLGPMATA 318

BLAST of CSPI06G22650 vs. NCBI nr
Match: BAI39989.1 (ACC oxidase [Cucumis melo])

HSP 1 Score: 621.7 bits (1602), Expect = 3.5e-174
Identity = 309/319 (96.87%), Postives = 311/319 (97.49%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           MAVFPIINLENIN DGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDH KKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           LENLAEELLDLLCENLGLEKGYLK AFYGSKGPTFGTKVS YPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKV GLQLLKDG+WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL TQ
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVL-TQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVEK--DEEKKEVYPKFVFEDYMKLYLGVKFQAK 300
           TSGTGRMSIASFYNPGSDAVIYPAPA+VEK  DEEKKEVYPKFVFEDYMKLYLGVKFQAK
Sbjct: 241 TSGTGRMSIASFYNPGSDAVIYPAPAVVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAK 300

Query: 301 EPRFEAMKANANLGPMATA 318
           EPRFEAMKANANLGPMATA
Sbjct: 301 EPRFEAMKANANLGPMATA 318

BLAST of CSPI06G22650 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 494.6 bits (1272), Expect = 6.0e-140
Identity = 237/324 (73.15%), Postives = 280/324 (86.42%), Query Frame = 0

Query: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60
           M  FPIINLE +NG+ RA  +E+I+DAC+NWGFFE VNHGI  E LD VEKMT++H KKC
Sbjct: 1   MESFPIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKC 60

Query: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120
           MEERFKE++ ++GL++ ++EVND+DWESTF+L+HLP SNIS + DLD++Y+ +MK+FA K
Sbjct: 61  MEERFKESIKNRGLDSLRSEVNDVDWESTFYLKHLPVSNISDVPDLDDDYRTLMKDFAGK 120

Query: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180
           +E L+EELLDLLCENLGLEKGYLK  FYGSK PTFGTKVS YPPCP PDL+KGLRAHTDA
Sbjct: 121 IEKLSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPDLVKGLRAHTDA 180

Query: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240
           GGIILLFQDDKV GLQLLKDG+W+DVPP++H+IVVNLGDQLEVITNG+YKSV HRVL +Q
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEHRVL-SQ 240

Query: 241 TSGTGRMSIASFYNPGSDAVIYPAPALV--EKDEEKKEVYPKFVFEDYMKLYLGVKFQAK 300
           T G GRMSIASFYNPGSD+VI+PAP L+  E ++EKKE YP+FVFEDYMKLY  VKFQAK
Sbjct: 241 TDGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAVKFQAK 300

Query: 301 EPRFEAMKANA-----NLGPMATA 318
           EPRFEAMKA       N+GP+ATA
Sbjct: 301 EPRFEAMKAMETTVANNVGPLATA 323

BLAST of CSPI06G22650 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 455.7 bits (1171), Expect = 3.1e-128
Identity = 213/315 (67.62%), Postives = 260/315 (82.54%), Query Frame = 0

Query: 4   FPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKCMEE 63
           FP+++L  +NG+ R + +  I +AC+NWGFFE+VNHG+PH+ +D +EKMT+DH K C E+
Sbjct: 7   FPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQEQ 66

Query: 64  RFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKKLEN 123
           +F + + SKGL+  + EV D+DWESTF++RHLP+SN++ +SD+ +EY+  MK+F K+LEN
Sbjct: 67  KFNDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAMKDFGKRLEN 126

Query: 124 LAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDAGGI 183
           LAE+LLDLLCENLGLEKGYLK  F+G+KGPTFGTKVS YPPCPKP++IKGLRAHTDAGGI
Sbjct: 127 LAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAHTDAGGI 186

Query: 184 ILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQTSG 243
           ILLFQDDKV GLQLLKDGDWIDVPP+ H+IV+NLGDQLEVITNG+YKSV+HRV +TQ  G
Sbjct: 187 ILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKYKSVLHRV-VTQQEG 246

Query: 244 TGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEPRFE 303
             RMS+ASFYNPGSDA I PA +LVEKD E    YP FVF+DYMKLY GVKFQ KEPRF 
Sbjct: 247 -NRMSVASFYNPGSDAEISPATSLVEKDSE----YPSFVFDDYMKLYAGVKFQPKEPRFA 306

Query: 304 AMK---ANANLGPMA 316
           AMK   A   L P A
Sbjct: 307 AMKNASAVTELNPTA 315

BLAST of CSPI06G22650 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 449.9 bits (1156), Expect = 1.7e-126
Identity = 213/315 (67.62%), Postives = 262/315 (83.17%), Query Frame = 0

Query: 4   FPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKCMEE 63
           FP+I+L  +NG+ R + +  I+DACQNWGFFELVNHG+P++ +D +E+MT++H KK ME+
Sbjct: 7   FPVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKKHMEQ 66

Query: 64  RFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKKLEN 123
           +FKE + SKGL+  + EV D+DWESTF+L HLP+SN+  + D+  EY+  MK+F K+LE 
Sbjct: 67  KFKEMLRSKGLDTLETEVEDVDWESTFYLHHLPQSNLYDIPDMSNEYRLAMKDFGKRLEI 126

Query: 124 LAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDAGGI 183
           LAEELLDLLCENLGLEKGYLK  F+G+ GPTF TK+S YPPCPKP++IKGLRAHTDAGG+
Sbjct: 127 LAEELLDLLCENLGLEKGYLKKVFHGTTGPTFATKLSNYPPCPKPEMIKGLRAHTDAGGL 186

Query: 184 ILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQTSG 243
           ILLFQDDKV GLQLLKDGDW+DVPP++H+IV+NLGDQLEVITNG+YKSVMHRV+ TQ  G
Sbjct: 187 ILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMHRVM-TQKEG 246

Query: 244 TGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVKFQAKEPRFE 303
             RMSIASFYNPGSDA I PA +LV+KD +    YP FVF+DYMKLY G+KFQAKEPRFE
Sbjct: 247 -NRMSIASFYNPGSDAEISPATSLVDKDSK----YPSFVFDDYMKLYAGLKFQAKEPRFE 306

Query: 304 AMK---ANANLGPMA 316
           AMK   A A+L P+A
Sbjct: 307 AMKNAEAAADLNPVA 315

BLAST of CSPI06G22650 vs. TAIR 10
Match: AT2G19590.1 (ACC oxidase 1 )

HSP 1 Score: 302.8 bits (774), Expect = 3.4e-82
Identity = 147/308 (47.73%), Postives = 207/308 (67.21%), Query Frame = 0

Query: 5   PIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKCMEER 64
           P+I+   ++G+ R+K +  ++ AC  WGFF + NHGI  E ++ V+KM   H ++ ++E+
Sbjct: 12  PVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEHLKEK 71

Query: 65  FKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKKLENL 124
           F ++ + K L  ++ + +D DWES+FF+ H P SNI Q+ ++ EE  K M E+  +L   
Sbjct: 72  FYQSEMVKAL--SEGKTSDADWESSFFISHKPTSNICQIPNISEELSKTMDEYVCQLHKF 131

Query: 125 AEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDAGGII 184
           AE L  L+CENLGL++  + NAF G KGP FGTKV+KYP CP+P+L++GLR HTDAGGII
Sbjct: 132 AERLSKLMCENLGLDQEDIMNAFSGPKGPAFGTKVAKYPECPRPELMRGLREHTDAGGII 191

Query: 185 LLFQDDKVGGLQLLKDGDWIDVPPMR-HAIVVNLGDQLEVITNGRYKSVMHRVLITQTSG 244
           LL QDD+V GL+  KDG W+ +PP + + I VN GDQLE+++NGRYKSV+HRV+  +   
Sbjct: 192 LLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSVVHRVMTVKHG- 251

Query: 245 TGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPK-FVFEDYMKLYLGVKFQAKEPRF 304
             R+SIA+FYNP  DA+I PAP L         +YP  + F+DY+KLY   KF  K PR 
Sbjct: 252 -SRLSIATFYNPAGDAIISPAPKL---------LYPSGYRFQDYLKLYSTTKFGDKGPRL 306

Query: 305 EAMKANAN 311
           E MK   N
Sbjct: 312 ETMKKMGN 306

BLAST of CSPI06G22650 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 287.3 bits (734), Expect = 1.5e-77
Identity = 151/313 (48.24%), Postives = 204/313 (65.18%), Query Frame = 0

Query: 5   PIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKCMEER 64
           P+I+   +NG+ R K L +I  AC+ WGFF+LVNHGIP E L+ V+K++ D  K   EE 
Sbjct: 4   PVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTEREEA 63

Query: 65  FKET----VLSKGLEAAQAE-VNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAK 124
           FK +    +L++ ++    E + ++DWE  F L           ++     K+ M E+ +
Sbjct: 64  FKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLD------HNQNEWPSNIKETMGEYRE 123

Query: 125 KLENLAEELLDLLCENLGLEKGYLKNAFY-----GSKGPTFGTKVSKYPPCPKPDLIKGL 184
           ++  LA ++++++ ENLGL KGY+K AF      G +   FGTKVS YPPCP P+L+ GL
Sbjct: 124 EVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELVNGL 183

Query: 185 RAHTDAGGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMH 244
           RAHTDAGG++LLFQDD+  GLQ+LKDG+WIDV P+ +AIV+N GDQ+EV++NGRYKS  H
Sbjct: 184 RAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKSAWH 243

Query: 245 RVLITQTSGTGRMSIASFYNPGSDAVIYPAPALVEKDEEKKEVYPKFVFEDYMKLYLGVK 304
           RVL  +     R SIASFYNP   A I PA    E+  EKK  YPKFVF DYM +Y   K
Sbjct: 244 RVLAREEG--NRRSIASFYNPSYKAAIGPAAVAEEEGSEKK--YPKFVFGDYMDVYANQK 303

Query: 305 FQAKEPRFEAMKA 308
           F  KEPRF A+K+
Sbjct: 304 FMPKEPRFLAVKS 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q046442.1e-17797.181-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=ACO1 PE=2... [more]
P051164.2e-14680.191-aminocyclopropane-1-carboxylate oxidase 1 OS=Solanum lycopersicum OX=4081 GN=A... [more]
P241574.2e-14681.761-aminocyclopropane-1-carboxylate oxidase 4 OS=Solanum lycopersicum OX=4081 GN=A... [more]
Q085072.7e-14578.571-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 P... [more]
Q085066.7e-14478.441-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 P... [more]
Match NameE-valueIdentityDescription
O821261.3e-182100.00ACC oxidase OS=Cucumis sativus OX=3659 GN=CS-ACO1 PE=2 SV=1[more]
O649474.9e-18299.68ACC oxidase 3 OS=Cucumis sativus OX=3659 GN=Cs-ACO3 PE=2 SV=1[more]
A0A5D3C3Q01.2e-17597.491-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo var. makuwa OX=11946... [more]
D0V4H67.6e-17597.181-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=LOC103491... [more]
C8KIS11.7e-17496.87ACC oxidase OS=Cucumis melo OX=3656 GN=CM-ACO1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
NP_001267694.12.7e-182100.001-aminocyclopropane-1-carboxylate oxidase 1-like [Cucumis sativus] >KAE8647342.1... [more]
AAC67234.11.0e-18199.68ACC oxidase 3 [Cucumis sativus][more]
KAA0061654.12.4e-17597.491-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo var. makuwa] >TYK05852... [more]
NP_001284392.11.6e-17497.181-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo] >Q04644.1 RecName: Fu... [more]
BAI39989.13.5e-17496.87ACC oxidase [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G05010.16.0e-14073.15ethylene-forming enzyme [more]
AT1G62380.13.1e-12867.62ACC oxidase 2 [more]
AT1G12010.11.7e-12667.622-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G19590.13.4e-8247.73ACC oxidase 1 [more]
AT1G77330.11.5e-7748.242-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 103..134
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 4..307
NoneNo IPR availablePANTHERPTHR47990:SF63ACC OXIDASE 2coord: 4..307
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 4..305
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 161..255
e-value: 6.0E-29
score: 100.5
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 3..316
e-value: 2.9E-99
score: 334.3
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 5..94
e-value: 2.2E-12
score: 47.8
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 153..255
score: 14.089303

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G22650.1CSPI06G22650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009693 ethylene biosynthetic process
biological_process GO:0009835 fruit ripening
molecular_function GO:0009815 1-aminocyclopropane-1-carboxylate oxidase activity
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0046872 metal ion binding