CSPI06G20500 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G20500
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Aspartic proteinase nepenthesin-1
Location	Chr6: 18581848 .. 18582233 (+)
RNA-Seq Expression	CSPI06G20500
Synteny	CSPI06G20500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACTTCAGAGGATGCCTTTGGTCAGCAAGTATTGGGCGTGACCTCTGCTTTGAGCTCCCTTCCCACAAAAATAGTAAACTTGATGTGCCTGATTTGATATTTCATTTTGAAGGTCTTGACTTGAAGCTTCCAGTTGATAACTATATGGTCGTCGACGAGGAGCTAGGGATTACACGATTCACGATGGTGGCTGCAGGAGGTTGGTCGGTTTTAGGCAGCATGCAACATCAAAATATGTTGGTTCTTCATGATCTTGAAACAACATTTGTATCATTCATTCCCACACAATGTTCTCAACTCAATTACACTAGAAGTCAGAACTTGATCATTTGTATCATTTGAATAACATTGGTCCTACCAGTGCTCTGCTCCCAGTTAACTAG

mRNA sequence

ATGTACTTCAGAGGATGCCTTTGGTCAGCAAGTATTGGGCGTGACCTCTGCTTTGAGCTCCCTTCCCACAAAAATAGTAAACTTGATGTGCCTGATTTGATATTTCATTTTGAAGGTCTTGACTTGAAGCTTCCAGTTGATAACTATATGGTCGTCGACGAGGAGCTAGGGATTACACGATTCACGATGGTGGCTGCAGGAGAACTTGATCATTTGTATCATTTGAATAACATTGGTCCTACCAGTGCTCTGCTCCCAGTTAACTAG

Coding sequence (CDS)

ATGTACTTCAGAGGATGCCTTTGGTCAGCAAGTATTGGGCGTGACCTCTGCTTTGAGCTCCCTTCCCACAAAAATAGTAAACTTGATGTGCCTGATTTGATATTTCATTTTGAAGGTCTTGACTTGAAGCTTCCAGTTGATAACTATATGGTCGTCGACGAGGAGCTAGGGATTACACGATTCACGATGGTGGCTGCAGGAGAACTTGATCATTTGTATCATTTGAATAACATTGGTCCTACCAGTGCTCTGCTCCCAGTTAACTAG

Protein sequence

MYFRGCLWSASIGRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTMVAAGELDHLYHLNNIGPTSALLPVN*

Homology

[Close]

BLAST of CSPI06G20500 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.3e-04
Identity = 20/42 (47.62%), Postives = 28/42 (66.67%), Query Frame = 0


Query: 10  ASIGRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMV 52
           +S G DLCF+ PS   S L +P  + HF+G DL+LP +NY +
Sbjct: 347 SSSGFDLCFQTPSDP-SNLQIPTFVMHFDGGDLELPSENYFI 387

[Close]

BLAST of CSPI06G20500 vs. ExPASy TrEMBL
Match: A0A2N9HF56 (Peptidase A1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS40859 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 6.3e-10
Identity = 31/57 (54.39%), Postives = 40/57 (70.18%), Query Frame = 0


Query: 13  GRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTMVAAGEL 70
           G D+CF+LPS     + VP L+FHFEGLDL+LPV+NYM+ D +LG+    M A G L
Sbjct: 357 GLDVCFDLPSQDVKDIKVPKLVFHFEGLDLELPVENYMIADTKLGVVCLAMGATGRL 413

[Close]

BLAST of CSPI06G20500 vs. ExPASy TrEMBL
Match: A0A438C3C6 (Aspartic proteinase nepenthesin-1 OS=Vitis vinifera OX=29760 GN=nep1_8 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.8e-09
Identity = 33/64 (51.56%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 9   SASIGRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTMVAAGE 68
           S++ G DLCF LP    +++ VP LIFHF+GLDL LPV+NYMV D E+G+    + A G 
Sbjct: 440 SSTTGLDLCFHLPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGS 499

Query: 69  LDHL 73
           L  L
Sbjct: 500 LSIL 503

[Close]

BLAST of CSPI06G20500 vs. ExPASy TrEMBL
Match: F6H0S5 (Peptidase A1 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001g03090 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.1e-09
Identity = 32/61 (52.46%), Postives = 42/61 (68.85%), Query Frame = 0


Query: 9   SASIGRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTMVAAGE 68
           S++ G DLCF LP    +++ VP LIFHF+GLDL LPV+NYMV D E+G+    + A G 
Sbjct: 354 SSTTGLDLCFHLPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGS 413

Query: 69  L 70
           L
Sbjct: 414 L 414

[Close]

BLAST of CSPI06G20500 vs. ExPASy TrEMBL
Match: A0A438KMS9 (Aspartic proteinase nepenthesin-1 OS=Vitis vinifera OX=29760 GN=nep1_0 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.1e-09
Identity = 32/61 (52.46%), Postives = 42/61 (68.85%), Query Frame = 0


Query: 9   SASIGRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTMVAAGE 68
           S++ G DLCF LP    +++ VP LIFHF+GLDL LPV+NYMV D E+G+    + A G 
Sbjct: 354 SSTTGLDLCFHLPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGS 413

Query: 69  L 70
           L
Sbjct: 414 L 414

[Close]

BLAST of CSPI06G20500 vs. ExPASy TrEMBL
Match: A0A1S4C8X9 (aspartic proteinase nepenthesin-1-like isoform X2 OS=Nicotiana tabacum OX=4097 GN=LOC107816390 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.0e-09
Identity = 30/55 (54.55%), Postives = 39/55 (70.91%), Query Frame = 0


Query: 9   SASIGRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTM 64
           S+S G DLCF LPS+ N+ ++VP L+FHFEG DL LP DNYM+ D  +G+    M
Sbjct: 361 SSSTGLDLCFTLPSNTNN-IEVPKLVFHFEGADLDLPADNYMIADSSMGVACLAM 414

[Close]

BLAST of CSPI06G20500 vs. NCBI nr
Match: XP_038886455.1 (aspartic proteinase nepenthesin-2-like [Benincasa hispida])

HSP 1 Score: 100.9 bits (250), Expect = 5.8e-18
Identity = 49/63 (77.78%), Postives = 53/63 (84.13%), Query Frame = 0


Query: 7   LWSASIGRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTMVAA 66
           +WS SIG DLCF+LPSH NSKLDVPDLIFHFE LDLKLPV+NYMVV+EELGI    M AA
Sbjct: 337 IWS-SIGLDLCFQLPSHNNSKLDVPDLIFHFEDLDLKLPVENYMVVNEELGIVCLAMGAA 396

Query: 67  GEL 70
           G L
Sbjct: 397 GGL 398

[Close]

BLAST of CSPI06G20500 vs. NCBI nr
Match: KAG6655526.1 (hypothetical protein CIPAW_05G222900 [Carya illinoinensis])

HSP 1 Score: 75.5 bits (184), Expect = 2.6e-10
Identity = 31/59 (52.54%), Postives = 44/59 (74.58%), Query Frame = 0


Query: 9   SASIGRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTMVAAG 68
           S + G DLCFE+P+ +  +++VP+L FHFEGLDL LP +NYM+VDE++G+    M  AG
Sbjct: 351 SDNSGLDLCFEIPNGRAKQIEVPELTFHFEGLDLALPPENYMIVDEKMGLACLAMAPAG 409

[Close]

BLAST of CSPI06G20500 vs. NCBI nr
Match: XP_042979078.1 (aspartic proteinase nepenthesin-1-like [Carya illinoinensis] >KAG2709021.1 hypothetical protein I3760_05G219700 [Carya illinoinensis] >KAG6714738.1 hypothetical protein I3842_05G216700 [Carya illinoinensis] >KAG7980780.1 hypothetical protein I3843_05G200700 [Carya illinoinensis])

HSP 1 Score: 75.5 bits (184), Expect = 2.6e-10
Identity = 31/59 (52.54%), Postives = 44/59 (74.58%), Query Frame = 0


Query: 9   SASIGRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTMVAAG 68
           S + G DLCFE+P+ +  +++VP+L FHFEGLDL LP +NYM+VDE++G+    M  AG
Sbjct: 351 SDNSGLDLCFEIPNGRAKQIEVPELTFHFEGLDLALPPENYMIVDEKMGLACLAMAPAG 409

[Close]

BLAST of CSPI06G20500 vs. NCBI nr
Match: RVW17763.1 (Aspartic proteinase nepenthesin-1 [Vitis vinifera])

HSP 1 Score: 71.6 bits (174), Expect = 3.8e-09
Identity = 33/64 (51.56%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 9   SASIGRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTMVAAGE 68
           S++ G DLCF LP    +++ VP LIFHF+GLDL LPV+NYMV D E+G+    + A G 
Sbjct: 440 SSTTGLDLCFHLPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGS 499

Query: 69  LDHL 73
           L  L
Sbjct: 500 LSIL 503

[Close]

BLAST of CSPI06G20500 vs. NCBI nr
Match: XP_010664223.1 (PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera])

HSP 1 Score: 70.9 bits (172), Expect = 6.4e-09
Identity = 32/61 (52.46%), Postives = 42/61 (68.85%), Query Frame = 0


Query: 9   SASIGRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTMVAAGE 68
           S++ G DLCF LP    +++ VP LIFHF+GLDL LPV+NYMV D E+G+    + A G 
Sbjct: 354 SSTTGLDLCFHLPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGS 413

Query: 69  L 70
           L
Sbjct: 414 L 414

[Close]

BLAST of CSPI06G20500 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 58.5 bits (140), Expect = 3.1e-09
Identity = 27/55 (49.09%), Postives = 35/55 (63.64%), Query Frame = 0


Query: 9   SASIGRDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTM 64
           S S G DLCF+LP    + + VP +IFHF+G DL+LP +NYMV D   G+    M
Sbjct: 370 SGSTGLDLCFKLPDAAKN-IAVPKMIFHFKGADLELPGENYMVADSSTGVLCLAM 423

[Close]

BLAST of CSPI06G20500 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 42.7 bits (99), Expect = 1.8e-04
Identity = 22/64 (34.38%), Postives = 33/64 (51.56%), Query Frame = 0


Query: 15  DLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTMVAAGELDHLYH 74
           D CF+L +    K  VP ++ HF G D+ LP  NY++  +  G  +F    AG +  L  
Sbjct: 402 DTCFDLSNMNEVK--VPTVVLHFRGADVSLPATNYLIPVDTNG--KFCFAFAGTMGGLSI 461

Query: 75  LNNI 79
           + NI
Sbjct: 462 IGNI 461

[Close]

BLAST of CSPI06G20500 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 42.0 bits (97), Expect = 3.0e-04
Identity = 23/64 (35.94%), Postives = 32/64 (50.00%), Query Frame = 0


Query: 15  DLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGITRFTMVAAGELDHLYH 74
           D CF+L      K  VP ++FHF G ++ LP  NY++     G  RF    AG +  L  
Sbjct: 401 DTCFDLSGMTTVK--VPTVVFHFGGGEVSLPASNYLIPVNTEG--RFCFAFAGTMGSLSI 460

Query: 75  LNNI 79
           + NI
Sbjct: 461 IGNI 460

The following BLAST results are available for this feature:

BLAST of CSPI06G20500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q766C3	1.3e-04	47.62	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]

BLAST of CSPI06G20500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2N9HF56	6.3e-10	54.39	Peptidase A1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4...	[more]
A0A438C3C6	1.8e-09	51.56	Aspartic proteinase nepenthesin-1 OS=Vitis vinifera OX=29760 GN=nep1_8 PE=3 SV=1	[more]
F6H0S5	3.1e-09	52.46	Peptidase A1 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001...	[more]
A0A438KMS9	3.1e-09	52.46	Aspartic proteinase nepenthesin-1 OS=Vitis vinifera OX=29760 GN=nep1_0 PE=3 SV=1	[more]
A0A1S4C8X9	7.0e-09	54.55	aspartic proteinase nepenthesin-1-like isoform X2 OS=Nicotiana tabacum OX=4097 G...	[more]

BLAST of CSPI06G20500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886455.1	5.8e-18	77.78	aspartic proteinase nepenthesin-2-like [Benincasa hispida]	[more]
KAG6655526.1	2.6e-10	52.54	hypothetical protein CIPAW_05G222900 [Carya illinoinensis]	[more]
XP_042979078.1	2.6e-10	52.54	aspartic proteinase nepenthesin-1-like [Carya illinoinensis] >KAG2709021.1 hypot...	[more]
RVW17763.1	3.8e-09	51.56	Aspartic proteinase nepenthesin-1 [Vitis vinifera]	[more]
XP_010664223.1	6.4e-09	52.46	PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera]	[more]

BLAST of CSPI06G20500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G03200.1	3.1e-09	49.09	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	1.8e-04	34.38	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	3.0e-04	35.94	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 8..81 e-value: 3.5E-7 score: 31.7
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 8..72

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G20500.1	CSPI06G20500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0005488	binding
molecular_function	GO:0008233	peptidase activity