CSPI06G19270 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G19270
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Separase
Location	Chr6: 17416752 .. 17419062 (-)
RNA-Seq Expression	CSPI06G19270
Synteny	CSPI06G19270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATCAGCCAAGCATTCTGACGGTTCTTCTGCAATCCGTTCCCTAGCTAAGGAGTTTCTCTCTTTTCTCAATCGCGCTCTCTCCATTCTCCCCAAACGCCTCACCAATCCTTCTAAACTGGGCAACCATCTCGATTTCGCCCTAGAGTTATTTCAAACCTATAAGCTTTGCTTGGGTTGTTTGGAATCTTTGACGTCGCAGCTTTCGTGTAAACCCTATTCTGTCGATGTCCAGAGGATTCGGATGGTGCATTGCAAGGAGGATTGGGGACTCTTCAAGGATGCGGAGGCAGATGGGTTTAGGATTTTGCAGAGGCTTGGGATAAAGGTAGTGGTGATGAAGGTTTTTGTTTGCTATTCGTGGAGGTCGTTGCAACGGTTGTTAAGTGTACGGCATGTGGTCGTAGTAAGGAAAGTGGCGACTATCGTAGGGTTCTTGGTTTGGTGGAGGAGGTTAGACCATGGTTCAGGTAAGAACTTCACTGTTTAAGTATCAAAGTTGATACCATTTTCGTTTCCCACCTTTTTAATATGGTGGCTTAGCTCGTAGCTAGATTGGAGTATATAGAAATTGTTGAAGGCAAGATTTGTTAGTGGAAAAGTTACTGAAATTGCAGGTTTCATACTAGTCCAGACCCCAGACCCAGTAGACAGTTTATTGATCTGGTATACACATTCCTTCGGCAGGTTTCTAGATGCTAAAGTATCCGAGAAGACTCAGAGAGCAGTGGTGACATATCTAGGCAAGTGTACTATTTTCTTGGCAGAGGAGCTAGTTTGTTTTGGTGAGAGTCTCGTAAGTCTCTTCTGCCTCACAACATTTGCTGAATATGCCAAGTCGCCTTTAAGAGATCAGATTTACAAGGTGATGTATCAACTTTTTTCTTCTTCTTTGTGGATACTATATTATATGCTAACATTGTTCTTTATTTATTTTCAGTTGGCCCGTCGGATATGTTCCATCCTTTTCTCTCTACAACAGGAATATCATACATCTGTGCTCGTCACAAACATACTAGCATGTGTCTTGAAGTCTCTTACCCTAGAAATAGAATGGAAGGTATAGTTAATGGAGAAGTTACGCCTAAAATCTTTTCCAATACCGATACCACTAAATATATCTTTCATTGAGTGTATGATATCTTGATGGTGTATTAAAGGGTGTCAAAAGAAAGAATTAAGGCCAACTTTGTTTCCTCTATAGATTTTCTTTATATGGTACTTTGTTGTTGTGCAGCATAATTGTGTCAATGTAGAAGAGAAAGAATGCTTCTCTAAACCAGAGGCTGAGTTTGTTAAGTTTTCTTAGAGAATTCCTCACCATTTAGGTTCTCTCATTCCTTTATTAAAATGTTAGGACTGGTGACACGTTTGGGGAGAATGGAGAGAGTACGATTGAGAAAAGAGAGAAACTCCTTAACTAGGGAACGGATTGCAGAAGAATCCTCGGTAGGCTTGGCTGATTAAATGCATTTCTTGGGCTTTTTGATATCGGAGAAAGGATGAAGAAAATCGGAGACTGGGGAGAATATACCTTTGGAATTCGTTGTTTCGAGGATAGAGATGAGAGCGGAGTTTGTGAGTGACACCATTGCCGGTGAAATGAAATGGAGCAAACGCCGGAGGAGATGGACGGAGACTGACTGGCTGAGTTTCTTCTTAAGAACGCTAGTGGAGAAGCCCGCCAATTTAATTCATTTTGAATTCTTCTCTCTTTTATTTTCCCTTATTTTTCTGATTTCTTTTCTTCTTGTAAAGGGCTCTTCTTTTTTCTTTTAATTTTTTGCATTGAAAACGTATTATCTTGCTCTGTGGAATTCTAAGGTCTTGATTTCATGTGTTTATTCAATGATTTTATAGGTAACGTTAGAGCGGATAATTAGTTCGATTTTCTTTTTGGGATCGACCAATAAACAAAATTAGCTTTGTTTCTTCATCCTCTATCTATTTACAACGTTAGTTTTTAGTTTTCTAGCCTTTCCACCACCATGGAAAAGGTAAGATCTATAGTTTTCTAATTTGGAGATTGTGGAGGAACTTATTGTAATTTTGAAGTATTTTGATAATGTGAACTACATCTCTTGTTTGTGAGTTTTAGTTTGGATACATTTAAATATTGATCGGCTTCCCATGCTTAGACCGCATAGTTTGATGGTTTAGGTTAACATATATAATACATGGCTTATAATTATAGGTTAGTCTCTAAGAAAAGGGATAGACCACCTTTTGATTCAAAGGGAGAAGAAGAGGCAAAAATTGGAACAAAAATATCATTTGATTCGTCAATCCTTAAAAGACAAAATTGTTGA

mRNA sequence

ATGAAATCAGCCAAGCATTCTGACGGTTCTTCTGCAATCCGTTCCCTAGCTAAGGAGTTTCTCTCTTTTCTCAATCGCGCTCTCTCCATTCTCCCCAAACGCCTCACCAATCCTTCTAAACTGGGCAACCATCTCGATTTCGCCCTAGAGTTATTTCAAACCTATAAGCTTTGCTTGGGTTGTTTGGAATCTTTGACGTCGCAGCTTTCGTGTAAACCCTATTCTGTCGATGTCCAGAGGATTCGGATGGTGCATTGCAAGGAGGATTGGGGACTCTTCAAGGATGCGGAGGCAGATGGGTTTAGGATTTTGCAGAGGCTTGGGATAAAGGTAGTGGTGATGAAGGTCGTTGCAACGGTTGTTAAGTGTACGGCATGTGGTCGTAGTAAGGAAAGTGGCGACTATCGTAGGGTTCTTGGTTTGGTGGAGGAGGCAAGATTTGTTAGTGGAAAAGTTACTGAAATTGCAGGTTTCATACTAGTCCAGACCCCAGACCCAGTAGACAGTTTATTGATCTGGTATACACATTCCTTCGGCAGGTTTCTAGATGCTAAAGTATCCGAGAAGACTCAGAGAGCAGTGGTGACATATCTAGGCAAGTGTACTATTTTCTTGGCAGAGGAGCTAGTTTGTTTTGGTGAGAGTCTCGTAAGTCTCTTCTGCCTCACAACATTTGCTGAATATGCCAAGTCGCCTTTAAGAGATCAGATTTACAAGTTGGCCCGTCGGATATGTTCCATCCTTTTCTCTCTACAACAGGAATATCATACATCTGTGCTCGTCACAAACATACTAGCATGTGTCTTGAAGTCTCTTACCCTAGAAATAGAATGGAAGCATAATTGTGTCAATGTAGAAGAGAAAGAATGCTTCTCTAAACCAGAGGCTGAGTTTGTTAAAAAATCGGAGACTGGGGAGAATATACCTTTGGAATTCGTTGTTTCGAGGATAGAGATGAGAGCGGAGTTTGTGAGTGACACCATTGCCGGTGAAATGAAATGGAGCAAACGCCGGAGGAGATGGACGGAGACTGACTGGCTGACCTTTCCACCACCATGGAAAAGGTTAGTCTCTAAGAAAAGGGATAGACCACCTTTTGATTCAAAGGGAGAAGAAGAGGCAAAAATTGGAACAAAAATATCATTTGATTCGTCAATCCTTAAAAGACAAAATTGTTGA

Coding sequence (CDS)

ATGAAATCAGCCAAGCATTCTGACGGTTCTTCTGCAATCCGTTCCCTAGCTAAGGAGTTTCTCTCTTTTCTCAATCGCGCTCTCTCCATTCTCCCCAAACGCCTCACCAATCCTTCTAAACTGGGCAACCATCTCGATTTCGCCCTAGAGTTATTTCAAACCTATAAGCTTTGCTTGGGTTGTTTGGAATCTTTGACGTCGCAGCTTTCGTGTAAACCCTATTCTGTCGATGTCCAGAGGATTCGGATGGTGCATTGCAAGGAGGATTGGGGACTCTTCAAGGATGCGGAGGCAGATGGGTTTAGGATTTTGCAGAGGCTTGGGATAAAGGTAGTGGTGATGAAGGTCGTTGCAACGGTTGTTAAGTGTACGGCATGTGGTCGTAGTAAGGAAAGTGGCGACTATCGTAGGGTTCTTGGTTTGGTGGAGGAGGCAAGATTTGTTAGTGGAAAAGTTACTGAAATTGCAGGTTTCATACTAGTCCAGACCCCAGACCCAGTAGACAGTTTATTGATCTGGTATACACATTCCTTCGGCAGGTTTCTAGATGCTAAAGTATCCGAGAAGACTCAGAGAGCAGTGGTGACATATCTAGGCAAGTGTACTATTTTCTTGGCAGAGGAGCTAGTTTGTTTTGGTGAGAGTCTCGTAAGTCTCTTCTGCCTCACAACATTTGCTGAATATGCCAAGTCGCCTTTAAGAGATCAGATTTACAAGTTGGCCCGTCGGATATGTTCCATCCTTTTCTCTCTACAACAGGAATATCATACATCTGTGCTCGTCACAAACATACTAGCATGTGTCTTGAAGTCTCTTACCCTAGAAATAGAATGGAAGCATAATTGTGTCAATGTAGAAGAGAAAGAATGCTTCTCTAAACCAGAGGCTGAGTTTGTTAAAAAATCGGAGACTGGGGAGAATATACCTTTGGAATTCGTTGTTTCGAGGATAGAGATGAGAGCGGAGTTTGTGAGTGACACCATTGCCGGTGAAATGAAATGGAGCAAACGCCGGAGGAGATGGACGGAGACTGACTGGCTGACCTTTCCACCACCATGGAAAAGGTTAGTCTCTAAGAAAAGGGATAGACCACCTTTTGATTCAAAGGGAGAAGAAGAGGCAAAAATTGGAACAAAAATATCATTTGATTCGTCAATCCTTAAAAGACAAAATTGTTGA

Protein sequence

MKSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGCLESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRLGIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTEIAGFILVQTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGESLVSLFCLTTFAEYAKSPLRDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTLEIEWKHNCVNVEEKECFSKPEAEFVKKSETGENIPLEFVVSRIEMRAEFVSDTIAGEMKWSKRRRRWTETDWLTFPPPWKRLVSKKRDRPPFDSKGEEEAKIGTKISFDSSILKRQNC*

Homology

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BLAST of CSPI06G19270 vs. ExPASy Swiss-Prot
Match: Q5IBC5 (Separase OS=Arabidopsis thaliana OX=3702 GN=ESP1 PE=2 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 3.8e-40
Identity = 103/301 (34.22%), Postives = 163/301 (54.15%), Query Frame = 0


Query: 3   SAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGCL 62
           S K  D ++ IR+LAK+FL FLN+++S+LPKRL+  +      + AL+LF+ Y+LCL CL
Sbjct: 37  SRKKQDRATTIRALAKQFLPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCL 96

Query: 63  ESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRL--------------- 122
           E +++QL+CKP++V  QR+RM+HC + WGL+++   + F++L++L               
Sbjct: 97  ELVSAQLACKPHTVQSQRLRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRLLPE 156

Query: 123 ------GIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTEIAGFILV 182
                  + +VV+  VA + +  A  +  +   YR+VL L+E          E+ G++  
Sbjct: 157 VQDGDAEMALVVVDAVAAIFRAVAMSQQLDDKRYRKVLLLLE----------EVGGWL-- 216

Query: 183 QTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGESLVSLFC 242
                             R LDAKV EK  RA+VT +GKC + L  E   F   LV  FC
Sbjct: 217 ------------------RVLDAKVYEKLHRAMVTSMGKCAVSLVREAERFNGDLVISFC 276

Query: 243 LTTFAEYAKSPL-RDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTLEIEWKH 282
             T  E+ KS L +D++YK AR + S+LF  +    +  +  +I   VL+SL+ + E + 
Sbjct: 277 DLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMS--VTIDISMSVLRSLSCQFEDES 305

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BLAST of CSPI06G19270 vs. ExPASy TrEMBL
Match: A0A0A0KDL0 (Separase OS=Cucumis sativus OX=3659 GN=Csa_6G364113 PE=4 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 5.6e-111
Identity = 222/306 (72.55%), Postives = 235/306 (76.80%), Query Frame = 0


Query: 2   KSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGC 61
           KS K +D SS IRSLAKEFLSFLNRALSILPKRLT+PSKLGNHLDFALELF+ YKLCLGC
Sbjct: 43  KSVKPTDDSSTIRSLAKEFLSFLNRALSILPKRLTDPSKLGNHLDFALELFEIYKLCLGC 102

Query: 62  LESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRL-------------- 121
           LE LTSQLSCKPY+VDVQRIRMVHC EDWGLFKDAEA+GFRIL RL              
Sbjct: 103 LECLTSQLSCKPYTVDVQRIRMVHCMEDWGLFKDAEAEGFRILDRLRDIDRRSKAGKLDF 162

Query: 122 -------------GIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTE 181
                        G  ++ ++VVATVVKCTACGRSKESGDYRRVLGLVEE R        
Sbjct: 163 RVIHDGDKGGGDEGFCLLFVEVVATVVKCTACGRSKESGDYRRVLGLVEEVR-------- 222

Query: 182 IAGFILVQTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGE 241
                             W+     RFLDAKVSEKTQRA+VTYLGKCTIFLAEELVCFGE
Sbjct: 223 -----------------TWF-----RFLDAKVSEKTQRALVTYLGKCTIFLAEELVCFGE 282

Query: 242 SLVSLFCLTTFAEYAKSPLRDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTL 281
           SLVSLFCLTTFAEYAKS LRDQIYKLARRICSILFSLQQE+HTS+LVTNILACVLKSLTL
Sbjct: 283 SLVSLFCLTTFAEYAKSSLRDQIYKLARRICSILFSLQQEHHTSMLVTNILACVLKSLTL 318

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BLAST of CSPI06G19270 vs. ExPASy TrEMBL
Match: A0A5A7VCK1 (Separase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00310 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 9.9e-108
Identity = 222/316 (70.25%), Postives = 237/316 (75.00%), Query Frame = 0


Query: 2   KSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGC 61
           KSAK +D SSAIRSLAKEFLSFLNRALSILPKRL+NPSKLGN LDFALELF+ YKLCLGC
Sbjct: 43  KSAKPTDDSSAIRSLAKEFLSFLNRALSILPKRLSNPSKLGNDLDFALELFEIYKLCLGC 102

Query: 62  LESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRL-------------- 121
           LESLTSQLSCKPY+VDVQRIRMVHC EDWGLFKDAEA+GFRIL RL              
Sbjct: 103 LESLTSQLSCKPYTVDVQRIRMVHCMEDWGLFKDAEAEGFRILGRLRDIDSRSKAGKMDC 162

Query: 122 -------------GIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTE 181
                        G  ++ ++VVATVVKCTACGRSKESGDYRRVLGLVEE R        
Sbjct: 163 RVIHDGDKGGGDEGFCLLFVEVVATVVKCTACGRSKESGDYRRVLGLVEEVR-------- 222

Query: 182 IAGFILVQTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGE 241
                             W+     RFLDAKVSEKTQRA+VTYLGKCTIFLAEELVCFG 
Sbjct: 223 -----------------PWF-----RFLDAKVSEKTQRALVTYLGKCTIFLAEELVCFGG 282

Query: 242 SLVSLFCLTTFAEYAKSPLRDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTL 291
           SLVSLFCL TFAEYAKS LRDQ YKLARRICSILFSLQQE+HTS+LVT+ILACVLKSLTL
Sbjct: 283 SLVSLFCLATFAEYAKSSLRDQTYKLARRICSILFSLQQEHHTSMLVTSILACVLKSLTL 322

[Close]

BLAST of CSPI06G19270 vs. ExPASy TrEMBL
Match: A0A1S4DV87 (Separase OS=Cucumis melo OX=3656 GN=LOC103488503 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 9.9e-108
Identity = 221/316 (69.94%), Postives = 238/316 (75.32%), Query Frame = 0


Query: 2   KSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGC 61
           KSAK ++ SSAIRSLAKEFLSFLNRALSILPKRL+NPSKLGN LDFALELF+ YKLCLGC
Sbjct: 43  KSAKPTEDSSAIRSLAKEFLSFLNRALSILPKRLSNPSKLGNDLDFALELFEIYKLCLGC 102

Query: 62  LESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRL-------------- 121
           LESLTSQLSCKPY+VDVQRIRMVHC EDWGLFKDAEA+GFRIL+RL              
Sbjct: 103 LESLTSQLSCKPYTVDVQRIRMVHCMEDWGLFKDAEAEGFRILERLRDIDSRSKAGKMDC 162

Query: 122 -------------GIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTE 181
                        G  ++ ++VVATVVKCTACGRSKESGDYRRVLGLVEE R        
Sbjct: 163 RVIHDGDKGGGDEGFCLLFVEVVATVVKCTACGRSKESGDYRRVLGLVEEVR-------- 222

Query: 182 IAGFILVQTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGE 241
                             W+     RFLDAKVSEKTQRA+VTYLGKCTIFLAEELVCFG 
Sbjct: 223 -----------------PWF-----RFLDAKVSEKTQRALVTYLGKCTIFLAEELVCFGG 282

Query: 242 SLVSLFCLTTFAEYAKSPLRDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTL 291
           SLVSLFCL TFAEYAKS LRDQ YKLARRICSILFSLQQE+HTS+LVT+ILACVLKSLTL
Sbjct: 283 SLVSLFCLATFAEYAKSSLRDQTYKLARRICSILFSLQQEHHTSMLVTSILACVLKSLTL 322

[Close]

BLAST of CSPI06G19270 vs. ExPASy TrEMBL
Match: A0A1S3BCF1 (Separase OS=Cucumis melo OX=3656 GN=LOC103488503 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 9.9e-108
Identity = 221/316 (69.94%), Postives = 238/316 (75.32%), Query Frame = 0


Query: 2   KSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGC 61
           KSAK ++ SSAIRSLAKEFLSFLNRALSILPKRL+NPSKLGN LDFALELF+ YKLCLGC
Sbjct: 43  KSAKPTEDSSAIRSLAKEFLSFLNRALSILPKRLSNPSKLGNDLDFALELFEIYKLCLGC 102

Query: 62  LESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRL-------------- 121
           LESLTSQLSCKPY+VDVQRIRMVHC EDWGLFKDAEA+GFRIL+RL              
Sbjct: 103 LESLTSQLSCKPYTVDVQRIRMVHCMEDWGLFKDAEAEGFRILERLRDIDSRSKAGKMDC 162

Query: 122 -------------GIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTE 181
                        G  ++ ++VVATVVKCTACGRSKESGDYRRVLGLVEE R        
Sbjct: 163 RVIHDGDKGGGDEGFCLLFVEVVATVVKCTACGRSKESGDYRRVLGLVEEVR-------- 222

Query: 182 IAGFILVQTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGE 241
                             W+     RFLDAKVSEKTQRA+VTYLGKCTIFLAEELVCFG 
Sbjct: 223 -----------------PWF-----RFLDAKVSEKTQRALVTYLGKCTIFLAEELVCFGG 282

Query: 242 SLVSLFCLTTFAEYAKSPLRDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTL 291
           SLVSLFCL TFAEYAKS LRDQ YKLARRICSILFSLQQE+HTS+LVT+ILACVLKSLTL
Sbjct: 283 SLVSLFCLATFAEYAKSSLRDQTYKLARRICSILFSLQQEHHTSMLVTSILACVLKSLTL 322

[Close]

BLAST of CSPI06G19270 vs. ExPASy TrEMBL
Match: A0A1S3BCW7 (Separase OS=Cucumis melo OX=3656 GN=LOC103488503 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 9.9e-108
Identity = 221/316 (69.94%), Postives = 238/316 (75.32%), Query Frame = 0


Query: 2   KSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGC 61
           KSAK ++ SSAIRSLAKEFLSFLNRALSILPKRL+NPSKLGN LDFALELF+ YKLCLGC
Sbjct: 43  KSAKPTEDSSAIRSLAKEFLSFLNRALSILPKRLSNPSKLGNDLDFALELFEIYKLCLGC 102

Query: 62  LESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRL-------------- 121
           LESLTSQLSCKPY+VDVQRIRMVHC EDWGLFKDAEA+GFRIL+RL              
Sbjct: 103 LESLTSQLSCKPYTVDVQRIRMVHCMEDWGLFKDAEAEGFRILERLRDIDSRSKAGKMDC 162

Query: 122 -------------GIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTE 181
                        G  ++ ++VVATVVKCTACGRSKESGDYRRVLGLVEE R        
Sbjct: 163 RVIHDGDKGGGDEGFCLLFVEVVATVVKCTACGRSKESGDYRRVLGLVEEVR-------- 222

Query: 182 IAGFILVQTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGE 241
                             W+     RFLDAKVSEKTQRA+VTYLGKCTIFLAEELVCFG 
Sbjct: 223 -----------------PWF-----RFLDAKVSEKTQRALVTYLGKCTIFLAEELVCFGG 282

Query: 242 SLVSLFCLTTFAEYAKSPLRDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTL 291
           SLVSLFCL TFAEYAKS LRDQ YKLARRICSILFSLQQE+HTS+LVT+ILACVLKSLTL
Sbjct: 283 SLVSLFCLATFAEYAKSSLRDQTYKLARRICSILFSLQQEHHTSMLVTSILACVLKSLTL 322

[Close]

BLAST of CSPI06G19270 vs. NCBI nr
Match: KAE8647229.1 (hypothetical protein Csa_019082 [Cucumis sativus])

HSP 1 Score: 503.8 bits (1296), Expect = 1.3e-138
Identity = 263/302 (87.09%), Postives = 267/302 (88.41%), Query Frame = 0


Query: 1   MKSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLG 60
           MKSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLG
Sbjct: 1   MKSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLG 60

Query: 61  CLESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRLGIKVVVMKVVATV 120
           CLESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRLGIKVVVMKVVATV
Sbjct: 61  CLESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRLGIKVVVMKVVATV 120

Query: 121 VKCTACGRSKESGDYRRVLGLVEEARFVSGKVTEIAGFILVQTPDPVDSLLIWYTHSFGR 180
           VKCT CGRSKESGDYRRVLGLVEE                         +  W+     R
Sbjct: 121 VKCTGCGRSKESGDYRRVLGLVEE------------------------EVRPWF-----R 180

Query: 181 FLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGESLVSLFCLTTFAEYAKSPLRDQIYKL 240
           FLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGESLVSLFCLTTFAEYAK PLRDQIYKL
Sbjct: 181 FLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGESLVSLFCLTTFAEYAKWPLRDQIYKL 240

Query: 241 ARRICSILFSLQQEYHTSVLVTNILACVLKSLTLEIEWKHNCVNVEEKECFSKPEAEFVK 300
           ARRICSILFSLQQEYHTS+LVTNILACVLKSLTLEIEWKHNCVNVEE+ECFSKPEAEFVK
Sbjct: 241 ARRICSILFSLQQEYHTSMLVTNILACVLKSLTLEIEWKHNCVNVEEQECFSKPEAEFVK 273

Query: 301 KS 303
            S
Sbjct: 301 FS 273

[Close]

BLAST of CSPI06G19270 vs. NCBI nr
Match: XP_011657383.1 (separase isoform X1 [Cucumis sativus] >XP_031743001.1 separase isoform X1 [Cucumis sativus] >XP_031743002.1 separase isoform X1 [Cucumis sativus])

HSP 1 Score: 408.3 bits (1048), Expect = 7.5e-110
Identity = 224/316 (70.89%), Postives = 238/316 (75.32%), Query Frame = 0


Query: 2   KSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGC 61
           KS K +D SS IRSLAKEFLSFLNRALSILPKRLT+PSKLGNHLDFALELF+ YKLCLGC
Sbjct: 43  KSVKPTDDSSTIRSLAKEFLSFLNRALSILPKRLTDPSKLGNHLDFALELFEIYKLCLGC 102

Query: 62  LESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRL-------------- 121
           LE LTSQLSCKPY+VDVQRIRMVHC EDWGLFKDAEA+GFRIL RL              
Sbjct: 103 LECLTSQLSCKPYTVDVQRIRMVHCMEDWGLFKDAEAEGFRILDRLRDIDRRSKAGKLDF 162

Query: 122 -------------GIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTE 181
                        G  ++ ++VVATVVKCTACGRSKESGDYRRVLGLVEE R        
Sbjct: 163 RVIHDGDKGGGDEGFCLLFVEVVATVVKCTACGRSKESGDYRRVLGLVEEVR-------- 222

Query: 182 IAGFILVQTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGE 241
                             W+     RFLDAKVSEKTQRA+VTYLGKCTIFLAEELVCFGE
Sbjct: 223 -----------------TWF-----RFLDAKVSEKTQRALVTYLGKCTIFLAEELVCFGE 282

Query: 242 SLVSLFCLTTFAEYAKSPLRDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTL 291
           SLVSLFCLTTFAEYAKS LRDQIYKLARRICSILFSLQQE+HTS+LVTNILACVLKSLTL
Sbjct: 283 SLVSLFCLTTFAEYAKSSLRDQIYKLARRICSILFSLQQEHHTSMLVTNILACVLKSLTL 322

[Close]

BLAST of CSPI06G19270 vs. NCBI nr
Match: XP_011657384.1 (separase isoform X2 [Cucumis sativus] >KAE8647231.1 hypothetical protein Csa_019070 [Cucumis sativus])

HSP 1 Score: 408.3 bits (1048), Expect = 7.5e-110
Identity = 224/316 (70.89%), Postives = 238/316 (75.32%), Query Frame = 0


Query: 2   KSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGC 61
           KS K +D SS IRSLAKEFLSFLNRALSILPKRLT+PSKLGNHLDFALELF+ YKLCLGC
Sbjct: 43  KSVKPTDDSSTIRSLAKEFLSFLNRALSILPKRLTDPSKLGNHLDFALELFEIYKLCLGC 102

Query: 62  LESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRL-------------- 121
           LE LTSQLSCKPY+VDVQRIRMVHC EDWGLFKDAEA+GFRIL RL              
Sbjct: 103 LECLTSQLSCKPYTVDVQRIRMVHCMEDWGLFKDAEAEGFRILDRLRDIDRRSKAGKLDF 162

Query: 122 -------------GIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTE 181
                        G  ++ ++VVATVVKCTACGRSKESGDYRRVLGLVEE R        
Sbjct: 163 RVIHDGDKGGGDEGFCLLFVEVVATVVKCTACGRSKESGDYRRVLGLVEEVR-------- 222

Query: 182 IAGFILVQTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGE 241
                             W+     RFLDAKVSEKTQRA+VTYLGKCTIFLAEELVCFGE
Sbjct: 223 -----------------TWF-----RFLDAKVSEKTQRALVTYLGKCTIFLAEELVCFGE 282

Query: 242 SLVSLFCLTTFAEYAKSPLRDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTL 291
           SLVSLFCLTTFAEYAKS LRDQIYKLARRICSILFSLQQE+HTS+LVTNILACVLKSLTL
Sbjct: 283 SLVSLFCLTTFAEYAKSSLRDQIYKLARRICSILFSLQQEHHTSMLVTNILACVLKSLTL 322

[Close]

BLAST of CSPI06G19270 vs. NCBI nr
Match: XP_011657385.1 (separase isoform X3 [Cucumis sativus])

HSP 1 Score: 408.3 bits (1048), Expect = 7.5e-110
Identity = 224/316 (70.89%), Postives = 238/316 (75.32%), Query Frame = 0


Query: 2   KSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGC 61
           KS K +D SS IRSLAKEFLSFLNRALSILPKRLT+PSKLGNHLDFALELF+ YKLCLGC
Sbjct: 43  KSVKPTDDSSTIRSLAKEFLSFLNRALSILPKRLTDPSKLGNHLDFALELFEIYKLCLGC 102

Query: 62  LESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRL-------------- 121
           LE LTSQLSCKPY+VDVQRIRMVHC EDWGLFKDAEA+GFRIL RL              
Sbjct: 103 LECLTSQLSCKPYTVDVQRIRMVHCMEDWGLFKDAEAEGFRILDRLRDIDRRSKAGKLDF 162

Query: 122 -------------GIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTE 181
                        G  ++ ++VVATVVKCTACGRSKESGDYRRVLGLVEE R        
Sbjct: 163 RVIHDGDKGGGDEGFCLLFVEVVATVVKCTACGRSKESGDYRRVLGLVEEVR-------- 222

Query: 182 IAGFILVQTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGE 241
                             W+     RFLDAKVSEKTQRA+VTYLGKCTIFLAEELVCFGE
Sbjct: 223 -----------------TWF-----RFLDAKVSEKTQRALVTYLGKCTIFLAEELVCFGE 282

Query: 242 SLVSLFCLTTFAEYAKSPLRDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTL 291
           SLVSLFCLTTFAEYAKS LRDQIYKLARRICSILFSLQQE+HTS+LVTNILACVLKSLTL
Sbjct: 283 SLVSLFCLTTFAEYAKSSLRDQIYKLARRICSILFSLQQEHHTSMLVTNILACVLKSLTL 322

[Close]

BLAST of CSPI06G19270 vs. NCBI nr
Match: XP_016899899.1 (PREDICTED: separase isoform X1 [Cucumis melo])

HSP 1 Score: 400.2 bits (1027), Expect = 2.0e-107
Identity = 221/316 (69.94%), Postives = 238/316 (75.32%), Query Frame = 0


Query: 2   KSAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGC 61
           KSAK ++ SSAIRSLAKEFLSFLNRALSILPKRL+NPSKLGN LDFALELF+ YKLCLGC
Sbjct: 43  KSAKPTEDSSAIRSLAKEFLSFLNRALSILPKRLSNPSKLGNDLDFALELFEIYKLCLGC 102

Query: 62  LESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRL-------------- 121
           LESLTSQLSCKPY+VDVQRIRMVHC EDWGLFKDAEA+GFRIL+RL              
Sbjct: 103 LESLTSQLSCKPYTVDVQRIRMVHCMEDWGLFKDAEAEGFRILERLRDIDSRSKAGKMDC 162

Query: 122 -------------GIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTE 181
                        G  ++ ++VVATVVKCTACGRSKESGDYRRVLGLVEE R        
Sbjct: 163 RVIHDGDKGGGDEGFCLLFVEVVATVVKCTACGRSKESGDYRRVLGLVEEVR-------- 222

Query: 182 IAGFILVQTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGE 241
                             W+     RFLDAKVSEKTQRA+VTYLGKCTIFLAEELVCFG 
Sbjct: 223 -----------------PWF-----RFLDAKVSEKTQRALVTYLGKCTIFLAEELVCFGG 282

Query: 242 SLVSLFCLTTFAEYAKSPLRDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTL 291
           SLVSLFCL TFAEYAKS LRDQ YKLARRICSILFSLQQE+HTS+LVT+ILACVLKSLTL
Sbjct: 283 SLVSLFCLATFAEYAKSSLRDQTYKLARRICSILFSLQQEHHTSMLVTSILACVLKSLTL 322

[Close]

BLAST of CSPI06G19270 vs. TAIR 10
Match: AT4G22970.1 (homolog of separase )

HSP 1 Score: 167.2 bits (422), Expect = 2.7e-41
Identity = 103/301 (34.22%), Postives = 163/301 (54.15%), Query Frame = 0


Query: 3   SAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGCL 62
           S K  D ++ IR+LAK+FL FLN+++S+LPKRL+  +      + AL+LF+ Y+LCL CL
Sbjct: 37  SRKKQDRATTIRALAKQFLPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCL 96

Query: 63  ESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRL--------------- 122
           E +++QL+CKP++V  QR+RM+HC + WGL+++   + F++L++L               
Sbjct: 97  ELVSAQLACKPHTVQSQRLRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRLLPE 156

Query: 123 ------GIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTEIAGFILV 182
                  + +VV+  VA + +  A  +  +   YR+VL L+E          E+ G++  
Sbjct: 157 VQDGDAEMALVVVDAVAAIFRAVAMSQQLDDKRYRKVLLLLE----------EVGGWL-- 216

Query: 183 QTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGESLVSLFC 242
                             R LDAKV EK  RA+VT +GKC + L  E   F   LV  FC
Sbjct: 217 ------------------RVLDAKVYEKLHRAMVTSMGKCAVSLVREAERFNGDLVISFC 276

Query: 243 LTTFAEYAKSPL-RDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTLEIEWKH 282
             T  E+ KS L +D++YK AR + S+LF  +    +  +  +I   VL+SL+ + E + 
Sbjct: 277 DLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMS--VTIDISMSVLRSLSCQFEDES 305

[Close]

BLAST of CSPI06G19270 vs. TAIR 10
Match: AT4G22970.2 (homolog of separase )

HSP 1 Score: 167.2 bits (422), Expect = 2.7e-41
Identity = 103/301 (34.22%), Postives = 163/301 (54.15%), Query Frame = 0


Query: 3   SAKHSDGSSAIRSLAKEFLSFLNRALSILPKRLTNPSKLGNHLDFALELFQTYKLCLGCL 62
           S K  D ++ IR+LAK+FL FLN+++S+LPKRL+  +      + AL+LF+ Y+LCL CL
Sbjct: 37  SRKKQDRATTIRALAKQFLPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCL 96

Query: 63  ESLTSQLSCKPYSVDVQRIRMVHCKEDWGLFKDAEADGFRILQRL--------------- 122
           E +++QL+CKP++V  QR+RM+HC + WGL+++   + F++L++L               
Sbjct: 97  ELVSAQLACKPHTVQSQRLRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRLLPE 156

Query: 123 ------GIKVVVMKVVATVVKCTACGRSKESGDYRRVLGLVEEARFVSGKVTEIAGFILV 182
                  + +VV+  VA + +  A  +  +   YR+VL L+E          E+ G++  
Sbjct: 157 VQDGDAEMALVVVDAVAAIFRAVAMSQQLDDKRYRKVLLLLE----------EVGGWL-- 216

Query: 183 QTPDPVDSLLIWYTHSFGRFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGESLVSLFC 242
                             R LDAKV EK  RA+VT +GKC + L  E   F   LV  FC
Sbjct: 217 ------------------RVLDAKVYEKLHRAMVTSMGKCAVSLVREAERFNGDLVISFC 276

Query: 243 LTTFAEYAKSPL-RDQIYKLARRICSILFSLQQEYHTSVLVTNILACVLKSLTLEIEWKH 282
             T  E+ KS L +D++YK AR + S+LF  +    +  +  +I   VL+SL+ + E + 
Sbjct: 277 DLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMS--VTIDISMSVLRSLSCQFEDES 305

[Close]

BLAST of CSPI06G19270 vs. TAIR 10
Match: AT4G24300.1 (Peptidase C50, separase )

HSP 1 Score: 54.3 bits (129), Expect = 2.6e-07
Identity = 35/99 (35.35%), Postives = 56/99 (56.57%), Query Frame = 0


Query: 180 RFLDAKVSEKTQRAVVTYLGKCTIFLAEELVCFGESLVSLFCLTTFAEYAKSPL-RDQIY 239
           R+LDA    K  + V++ +G+C + +  E   F E+ V  FC++T  EY+ SPL +   Y
Sbjct: 36  RYLDANEHHKFLKLVLSDMGECALSVIREAERFDEAFVHSFCISTLEEYSVSPLPKCHFY 95

Query: 240 KLARRICSILFSLQQEYHTSVLVTNILACVLKSLTLEIE 278
           K +R++ S+LF   +E  TS L   I+  VLK +  E +
Sbjct: 96  KFSRQVLSLLFP-SKEAKTS-LNLKIMMSVLKFVAGEFQ 132

The following BLAST results are available for this feature:

BLAST of CSPI06G19270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5IBC5	3.8e-40	34.22	Separase OS=Arabidopsis thaliana OX=3702 GN=ESP1 PE=2 SV=1	[more]

BLAST of CSPI06G19270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KDL0	5.6e-111	72.55	Separase OS=Cucumis sativus OX=3659 GN=Csa_6G364113 PE=4 SV=1	[more]
A0A5A7VCK1	9.9e-108	70.25	Separase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00310 PE=4 S...	[more]
A0A1S4DV87	9.9e-108	69.94	Separase OS=Cucumis melo OX=3656 GN=LOC103488503 PE=4 SV=1	[more]
A0A1S3BCF1	9.9e-108	69.94	Separase OS=Cucumis melo OX=3656 GN=LOC103488503 PE=4 SV=1	[more]
A0A1S3BCW7	9.9e-108	69.94	Separase OS=Cucumis melo OX=3656 GN=LOC103488503 PE=4 SV=1	[more]

BLAST of CSPI06G19270 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8647229.1	1.3e-138	87.09	hypothetical protein Csa_019082 [Cucumis sativus]	[more]
XP_011657383.1	7.5e-110	70.89	separase isoform X1 [Cucumis sativus] >XP_031743001.1 separase isoform X1 [Cucum...	[more]
XP_011657384.1	7.5e-110	70.89	separase isoform X2 [Cucumis sativus] >KAE8647231.1 hypothetical protein Csa_019...	[more]
XP_011657385.1	7.5e-110	70.89	separase isoform X3 [Cucumis sativus]	[more]
XP_016899899.1	2.0e-107	69.94	PREDICTED: separase isoform X1 [Cucumis melo]	[more]

BLAST of CSPI06G19270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G22970.1	2.7e-41	34.22	homolog of separase	[more]
AT4G22970.2	2.7e-41	34.22	homolog of separase	[more]
AT4G24300.1	2.6e-07	35.35	Peptidase C50, separase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005314	Peptidase C50, separase	PANTHER	PTHR12792	EXTRA SPINDLE POLES 1-RELATED	coord: 7..286
None	No IPR available	PANTHER	PTHR12792:SF0	SEPARIN	coord: 7..286

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G19270.1	CSPI06G19270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007059	chromosome segregation
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004197	cysteine-type endopeptidase activity