CSPI06G17480 (gene) Cucumber (PI 183967) v1

BLAST of CSPI06G17480 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 8.0e-188
Identity = 318/575 (55.30%), Postives = 420/575 (73.04%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60
           MS G+ + E  K+KK+  IIS+SS++L++MVVAVT+GV+       +  DN G E   EI
Sbjct: 1   MSYGY-DDEDAKRKKRYVIISISSVLLISMVVAVTIGVS------VNKSDNAGDE---EI 60

Query: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120
           T+S KAI+ +C PTDYKETCE +L  +A +T+DP ELVK  F    + +++  K S T+ 
Sbjct: 61  TTSVKAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMI 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180
           EL KDPR   AL  C+EL++YAI +L++SFE +G F+  K+D+ +  L+IWLS  +++EQ
Sbjct: 121 ELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQ 180

Query: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDG 240
           TCLDGF+ T G+AG  +++ LK+A Q+T NGL +V E+S  LG +Q+P M+ RRLL    
Sbjct: 181 TCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLL---S 240

Query: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300
           +E PSW+    RRL+ A  + +KPD+VVAQDGSG+YKTIN AL  VP K N TFV+H+K 
Sbjct: 241 QEFPSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKE 300

Query: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360
           GIYKE V + + MTHL   GDGP KTV++GS ++ DGI T+KTAT + +G +F A+++ F
Sbjct: 301 GIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAF 360

Query: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420
           ENTAGA KHQAVA+RV +D SIF+NC+ DGYQDTLYAH+HRQFYRDCTISGTIDF+FG+A
Sbjct: 361 ENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDA 420

Query: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480
           A  FQNC L+VRKPL NQ C +TAHGR + +E T  + Q C  +G+P YL  K  +K YL
Sbjct: 421 AAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYL 480

Query: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540
           GRPWKEYSRTII+ + I D + PEGW PW+G+FGLNTLFY+EVQN G GA  +KRV W G
Sbjct: 481 GRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPG 540

Query: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGM 576
           IK ++ +    FTP ++I GDAWIP KG+PY  G+
Sbjct: 541 IKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CSPI06G17480 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 656.4 bits (1692), Expect = 3.1e-187
Identity = 310/578 (53.63%), Postives = 424/578 (73.36%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60
           MS G+   +  K+K++  +I++SS++L++MVVAVTVGV+      +H  D+ G     E+
Sbjct: 1   MSYGY--DDESKRKRRYIVITISSVLLISMVVAVTVGVSL----NKHDGDSKGK---AEV 60

Query: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120
            +S KA++ +C PTDY++TCE +L     NTTDP ELVK  F +  + + +A K S T+ 
Sbjct: 61  NASVKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180
           EL KD RT  AL  C+EL++YA+D+L+ SFE +G F+   LD+ + +L+IWLS A+++E+
Sbjct: 121 ELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEE 180

Query: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDG 240
           TCL+GF+ T G+AG  M++ LK+A ++T NGL I++E+S  +G +Q+PG++ RRLL +  
Sbjct: 181 TCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEG- 240

Query: 241 REMPSWVSDGKRRLMQAGAA--AMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHV 300
              PSWV    R+L+QA AA   +KPD+VVAQDGSG+YKTIN AL  VP K N TFV+H+
Sbjct: 241 --FPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHI 300

Query: 301 KAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDM 360
           KAG+YKE V + K M+HL   GDGP KT+++G+ N+ DGI T++TAT + +G  F A+++
Sbjct: 301 KAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNI 360

Query: 361 GFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFG 420
           GFENTAGA KHQAVA+RVQSD SIFFNC+ DGYQDTLY H+HRQF+RDCTISGTIDF+FG
Sbjct: 361 GFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFG 420

Query: 421 NAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKA 480
           +AA  FQNC L+VRKPL NQ C +TAHGR + +E T  +FQ C   G+P YL  K  +KA
Sbjct: 421 DAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKA 480

Query: 481 YLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKW 540
           YLGRPWKEYSRTII+ + I D +QP+GW PW+GDFGL TLFY+EVQN G G+  + RV W
Sbjct: 481 YLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTW 540

Query: 541 RGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 577
            GIK ++ +    FTP ++I GD WIP KG+PY++G++
Sbjct: 541 AGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of CSPI06G17480 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 3.0e-166
Identity = 281/574 (48.95%), Postives = 400/574 (69.69%), Query Frame = 0

Query: 9   EGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTP-EITSSTKAI 68
           +G+ KKKK  I  V + +LV MVVAV +  +         + +H  +  P +I ++T A+
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSR--------NTSHSEKIVPVQIKTATTAV 63

Query: 69  QAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTT--LKELAKD 128
           +A+C PTDYKETC  SL   + ++T P +L+K+GF +  RS+ ++IK ++     + A D
Sbjct: 64  EAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAAND 123

Query: 129 PRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTCLDG 188
             T  AL+ C +L+  A DDL +  +    F + +++DFV DL++WLSG++ Y+QTC+D 
Sbjct: 124 KDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDT 183

Query: 189 FENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSG------RRLLE-D 248
           FE T       MQ+  K+++++T+NGL ++  +S +LG   + G++G      R+LL  +
Sbjct: 184 FEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAE 243

Query: 249 DGREMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHV 308
           DG  +PSWV    RRLM A    +K ++VVA DGSG+YKTIN AL  VP  + K FVI++
Sbjct: 244 DG--IPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYI 303

Query: 309 KAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDG-IQTFKTATFSAIGANFYARD 368
           K G+Y E V + K MTH+T  GDGPTKT +TGSLN+  G ++T+ TAT +  G NF A++
Sbjct: 304 KQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKN 363

Query: 369 MGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVF 428
           +GFENTAG   HQAVALRV +D ++F+NCQIDGYQDTLY H+HRQF+RDCT+SGT+DF+F
Sbjct: 364 IGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIF 423

Query: 429 GNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINK 488
           G+     QNC +VVRKP+ +Q C++TA GR +++E T L+ Q+CH  G+PAY+P K+INK
Sbjct: 424 GDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINK 483

Query: 489 AYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVK 548
           AYLGRPWKE+SRTII+G+TIDD+I P GWLPW GDF LNTL+YAE +N G G+++++RVK
Sbjct: 484 AYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVK 543

Query: 549 WRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPY 572
           W GIK ++P+ A  FTP RF+ G+ WIP   +PY
Sbjct: 544 WPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of CSPI06G17480 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 545.4 bits (1404), Expect = 7.6e-154
Identity = 273/578 (47.23%), Postives = 388/578 (67.13%), Query Frame = 0

Query: 8   AEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEITSSTKAI 67
           ++GDKKKK I   SVS  +++ +V    V   ++P      D+NH       I  +TKA+
Sbjct: 3   SDGDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSP----KDDENH-------IRKTTKAV 62

Query: 68  QAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAI-KNSTTLKELA-KD 127
           QA+C PTD+K+TC  SL   + ++ DP +L+K+GFK+  +S+NE++ K S  +K  A K+
Sbjct: 63  QAVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKN 122

Query: 128 PRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTCLDG 187
           P    A + C +L+  AIDDL +  +    F + +++ FV DL++WLSG++ ++QTC+D 
Sbjct: 123 PEAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDS 182

Query: 188 FENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSG------RRLLEDD 247
           F     +    M +  K+++++++N L +V  +ST++ +  L G++G      R+LL  +
Sbjct: 183 FGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTE 242

Query: 248 GREMPSWVSDGKRRLMQA---GAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVI 307
              +P+WV    RRLM A   G   +K + VVAQDG+G++KTI  AL  VP  +   F+I
Sbjct: 243 D-SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFII 302

Query: 308 HVKAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNF-IDGIQTFKTATFSAIGANFYA 367
           H+K GIYKE V + K M H+T  GDGP KT++TGSLNF I  ++TF TAT +  G +F A
Sbjct: 303 HIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTA 362

Query: 368 RDMGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDF 427
           +++G ENTAG    QAVALRV +D ++F +CQIDG+QDTLY H+HRQFYRDCT+SGT+DF
Sbjct: 363 KNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDF 422

Query: 428 VFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAI 487
           +FG+A    QNCK+VVRKP   Q C+VTA GR N +E T L+   CH  GDPAY+P K++
Sbjct: 423 IFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSV 482

Query: 488 NKAYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKR 547
           NKAYLGRPWKE+SRTII+ +TIDD+I P GWLPW GDF L TL+YAE  N G G+++++R
Sbjct: 483 NKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQR 542

Query: 548 VKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSS 574
           VKW GIK +TPQ A  +T  RF+ GD WIP   +PY++
Sbjct: 543 VKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of CSPI06G17480 vs. ExPASy Swiss-Prot
Match: Q42920 (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 2.3e-134
Identity = 236/452 (52.21%), Postives = 311/452 (68.81%), Query Frame = 0

Query: 132 LQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTCLDGFENTTG 191
           ++ C E+L+YA+D +++S   +  F   KL ++  DLK+WL+G L+++QTCLDGF NTT 
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTT 60

Query: 192 DAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMS-GRRLLEDDGREMPSWVSDG 251
            AG  M + LK++ ++++N + +++ +S IL        S  RRLL DDG  +PSWV+DG
Sbjct: 61  KAGETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRLLSDDG--IPSWVNDG 120

Query: 252 KRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKAGIYKEIVVIP 311
            RRL+  G   ++P+ VVAQDGSG++KT+  AL  VP K+   FVIHVKAG+YKE V + 
Sbjct: 121 HRRLLAGG--NVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKETVNVA 180

Query: 312 KHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAGAAKHQ 371
           K M ++T+ GDGPTKT  TGSLN+ DGI T+ TATF   GANF A+D+GFENTAG  KHQ
Sbjct: 181 KEMNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGKHQ 240

Query: 372 AVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLV 431
           AVALRV +D++IF+NCQ+DG+QDTLY  + RQFYRDC+ISGTIDFVFG     FQNCKLV
Sbjct: 241 AVALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLV 300

Query: 432 VRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYLGRPWKEYSRT 491
            R P   QQC+VTA GR  +   +AL+FQS HF G+PA L       +YLGRPWK+YS+ 
Sbjct: 301 CRLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPA-LTSVTPKVSYLGRPWKQYSKV 360

Query: 492 IIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRGIKHITPQHAA 551
           +I+ STID I  PEG++PWMG     T  Y E  NKG GAD + RVKW G+K +T   AA
Sbjct: 361 VIMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTSNVAA 420

Query: 552 DFTPRRFID------GDAWIPAKGIPYSSGMM 577
           ++ P +F +       D WI   G+PYS G M
Sbjct: 421 EYYPGKFFEIVNATARDTWIVKSGVPYSLGPM 447

BLAST of CSPI06G17480 vs. ExPASy TrEMBL
Match: A0A0A0KD24 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1)

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 575/576 (99.83%), Postives = 575/576 (99.83%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60
           MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60

Query: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120
           TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK
Sbjct: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180
           ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ
Sbjct: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180

Query: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDG 240
           TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDD 
Sbjct: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDD 240

Query: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300
           REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA
Sbjct: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300

Query: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360
           GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF
Sbjct: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360

Query: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420
           ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA
Sbjct: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420

Query: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480
           ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL
Sbjct: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480

Query: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540
           GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG
Sbjct: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540

Query: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 577
           IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM
Sbjct: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of CSPI06G17480 vs. ExPASy TrEMBL
Match: A0A1S3C7B6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1)

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 540/576 (93.75%), Postives = 564/576 (97.92%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60
           MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVG+NNAP+ EEHG+ + GS+TTPEI
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPY-EEHGEGDQGSKTTPEI 60

Query: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120
           +SSTKAIQAICQPTDYKETCEKSL AEAGNTTDPKELVKVGFKIATRSLNEAI+NSTTL+
Sbjct: 61  SSSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQ 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180
           EL+KDPRTNQALQNCRELLEYAIDDLN+SFERIGTFQMSKLD+FVADLKIWLSGALTYEQ
Sbjct: 121 ELSKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQ 180

Query: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDG 240
           TCLDGFENTTGDAG RMQEFLKSAQ+MTTNGLGIVNELSTILGSLQ+PGM+GRRLLEDD 
Sbjct: 181 TCLDGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPGMTGRRLLEDDD 240

Query: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300
            EMPSW+SDGKRRLMQAGAA MKPDL+VAQDGSGKYKTINAALADVPLKSNKTFVI+VKA
Sbjct: 241 HEMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKA 300

Query: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360
           GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF
Sbjct: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360

Query: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420
           ENTAG+AKHQAVALRVQSDRSIF+NCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA
Sbjct: 361 ENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420

Query: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480
           ATNFQNCKLVVRKP+DNQQCIVTAHGR+NRKEPTALIFQ CHFMGDPAYLPFK+INKAYL
Sbjct: 421 ATNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAYL 480

Query: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540
           GRPWK+YSRTIIIGS IDDIIQPEGWLPWMGDFGLNTLFYAEVQNKG GA E+KRVKWRG
Sbjct: 481 GRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWRG 540

Query: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 577
           IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM
Sbjct: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

BLAST of CSPI06G17480 vs. ExPASy TrEMBL
Match: A0A6J1KZN0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499042 PE=3 SV=1)

HSP 1 Score: 953.4 bits (2463), Expect = 4.5e-274
Identity = 472/604 (78.15%), Postives = 522/604 (86.42%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGE-----------EHGD 60
           MSNGF +AEGDKKKKKIA+ISVSSLILVAMVVAVTVGVNNAPH +           +HGD
Sbjct: 1   MSNGF-DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPHEDLDDGGGGGDLHDHGD 60

Query: 61  D-----NHG------------SETTPEITSSTKAIQAICQPTDYKETCEKSLEAEAGNTT 120
           D     +HG             +   +I+SSTKAI+ ICQPTDYKETCE+SL  EAGNTT
Sbjct: 61  DGGDVHDHGDYGGGGGDAHDHGDDGEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTT 120

Query: 121 DPKELVKVGFKIATRSLNEAIKNSTTLKELAKDPRTNQALQNCRELLEYAIDDLNQSFER 180
           D KELVK GFKIA   LN AI+NSTTL+ELAKDPR NQALQNCRELLEYAIDDL+ S+ R
Sbjct: 121 DLKELVKAGFKIAVEKLNAAIRNSTTLQELAKDPRANQALQNCRELLEYAIDDLHDSYHR 180

Query: 181 IGTFQMSKLDDFVADLKIWLSGALTYEQTCLDGFENTTGDAGVRMQEFLKSAQQMTTNGL 240
           IGTF +SK ++FV DLKIWLS ALTYEQTCLDGFENTTGD+G RM+EFLKSAQ+MTTNGL
Sbjct: 181 IGTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGL 240

Query: 241 GIVNELSTILGSLQLPGMSGRRLLEDDGREMPSWVSDGKRRLMQAGAAAMKPDLVVAQDG 300
           GIV+ELS ILG+L  P  +GRRLL+ D   MP WVSDGKRRLMQ    A+KPDLVVAQDG
Sbjct: 241 GIVSELSNILGTLHEPAATGRRLLDTD---MPLWVSDGKRRLMQLETGAIKPDLVVAQDG 300

Query: 301 SGKYKTINAALADVPLKSNKTFVIHVKAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSL 360
           SGKYKTIN AL DVP+KSNKTFVI+VKAGIYKEIVVIPKHMTHLTMYGDGPTK+VVTG+L
Sbjct: 301 SGKYKTINEALVDVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNL 360

Query: 361 NFIDGIQTFKTATFSAIGANFYARDMGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQ 420
           NF+DGIQTFKTATF+AIGANF+ RDMGF N+AG  KHQAVALR+QSDR+I +NCQIDG+Q
Sbjct: 361 NFVDGIQTFKTATFAAIGANFFVRDMGFVNSAGPTKHQAVALRIQSDRAIIYNCQIDGFQ 420

Query: 421 DTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKE 480
           DTLYAHA+RQFYRDCTISGTIDFVFGN+A  FQNCKLVVRKPLDNQQCIVTAHGRLNR+E
Sbjct: 421 DTLYAHANRQFYRDCTISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRRE 480

Query: 481 PTALIFQSCHFMGDPAYLPFKAINKAYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGD 540
           PTALIFQ CHFMGDPAYLPFK +NKAYLGRPWK+YSRTII+GS IDD+IQPEGWLPWMGD
Sbjct: 481 PTALIFQGCHFMGDPAYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGD 540

Query: 541 FGLNTLFYAEVQNKGAGADESKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYS 577
           FGLNTLFYAEVQN+G GAD+S RVKWRGI+HITPQHAA +T RRFIDGDAWIP +G+PYS
Sbjct: 541 FGLNTLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAASYTARRFIDGDAWIPVRGVPYS 600

BLAST of CSPI06G17480 vs. ExPASy TrEMBL
Match: A0A6J1H4H8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.9e-273
Identity = 466/583 (79.93%), Postives = 514/583 (88.16%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPH-------GEEHGDDNHG 60
           MSNGF +AE DKKKKKIA+ISVSSLILVAMVVAVTVGVNNAP        G   GD +  
Sbjct: 1   MSNGF-DAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDH 60

Query: 61  SETTPEITSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAI 120
            +   +I+SSTKAI+ ICQPTDYKETCE+SL  EAGNTTDPKELVK GFKIA   LN AI
Sbjct: 61  GDDAEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAI 120

Query: 121 KNSTTLKELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLS 180
           +NSTTL+ELA+DPR NQALQNCRELLEYAIDDL+ S+ RIGTF +SK ++FV DLKIWLS
Sbjct: 121 RNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLS 180

Query: 181 GALTYEQTCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGR 240
            ALTYEQTCLDGFENTTGD+G RM+EFLKSAQ+MTTNGLGIV+ELS ILG+L  P  +GR
Sbjct: 181 AALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLDAPAATGR 240

Query: 241 RLLEDDGREMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKT 300
           RLL+ D   +P WVSDGKRRLMQ G  A+KPDLVVAQDGSGKYKTIN AL  VP+KSNKT
Sbjct: 241 RLLDTD---IPLWVSDGKRRLMQVGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKT 300

Query: 301 FVIHVKAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANF 360
           FVI+VKAGIYKEIVVIPKHMTHLTMYGDGPTK+VVTG+LNF+DGIQTFKTATF+AIGANF
Sbjct: 301 FVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANF 360

Query: 361 YARDMGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTI 420
           + RDMGF NTAG  KHQAVALRVQSDR+I +NCQIDGYQDTLYAHA+RQFYRDC ISGTI
Sbjct: 361 FVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTI 420

Query: 421 DFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFK 480
           DFVFGN+A  FQNCKLVVRKPLDNQQCIVTAHGRLNR+EPTALIFQ CHFMGDP+YLPFK
Sbjct: 421 DFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFK 480

Query: 481 AINKAYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADES 540
            +NKAYLGRPWK+YSRTII+GS IDD+IQPEGWLPWMGDF LNTLFYAEVQN+G GAD+S
Sbjct: 481 KVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQS 540

Query: 541 KRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 577
            RVKWRGI+HITPQHAA +T RRFIDGDAWIP +G+PYSSGMM
Sbjct: 541 HRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of CSPI06G17480 vs. ExPASy TrEMBL
Match: A0A6J1KJ31 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.9e-253
Identity = 436/576 (75.69%), Postives = 489/576 (84.90%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60
           MSNGF +AEGD KKKK+A + VSSL LVAMVVAVTVGVNN+ H  E   D HG +   EI
Sbjct: 1   MSNGF-DAEGDNKKKKMAFVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHG-DGAGEI 60

Query: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120
           +SSTKAIQ IC+PTDYKETCEKSL +E  NTTDP+ELVK+GFK+    LN AI+NSTTL+
Sbjct: 61  SSSTKAIQTICRPTDYKETCEKSLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQ 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180
            LAK+PRTNQALQNCRELLEYAIDD+  SF RIGTFQM+KLD FV DL IWLSGALTYEQ
Sbjct: 121 ALAKEPRTNQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSIWLSGALTYEQ 180

Query: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDG 240
           TCLDGFENTTGDAG RM+ FLKSAQ+MT+NGL IV EL  +LGSLQ    +GRRLL  D 
Sbjct: 181 TCLDGFENTTGDAGERMKMFLKSAQEMTSNGLAIVTELKNMLGSLQ-GSKAGRRLLAGD- 240

Query: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300
            E+PSWVS  KRRLMQ   +A+ P++VVAQDGSGKYKTIN AL DVP KSN TFVI+VKA
Sbjct: 241 -EVPSWVSSEKRRLMQLPRSAITPNVVVAQDGSGKYKTINEALVDVPKKSNITFVIYVKA 300

Query: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360
           GIYKE V+IPK +TH+TMYGDGPTKT+V+G+LNF+DGIQTFKTATF+ +GANF+ RDMGF
Sbjct: 301 GIYKETVLIPKALTHVTMYGDGPTKTIVSGALNFVDGIQTFKTATFAVVGANFFCRDMGF 360

Query: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420
           ENTAG+AKHQAVALRVQSDRSIFFNCQ+DGYQDTLYA  HRQFYRDC +SGTIDFVFGN+
Sbjct: 361 ENTAGSAKHQAVALRVQSDRSIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNS 420

Query: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480
           A  FQNC LVVRKPL NQQCIVTA GRL  +EPT  +FQ CH MGDPAYLPFKA+NKAYL
Sbjct: 421 AAVFQNCNLVVRKPLANQQCIVTAQGRLEAREPTGFVFQKCHLMGDPAYLPFKAVNKAYL 480

Query: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540
           GRPWK+YSRTII+GS IDD+IQPEGWLPWMGDFGLN+LFYAE QNKG GAD + RVKW+G
Sbjct: 481 GRPWKQYSRTIIMGSEIDDLIQPEGWLPWMGDFGLNSLFYAEFQNKGPGADLTNRVKWQG 540

Query: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 577
           IK +T Q AADFTP+ FIDGDAWI   G+PYS GMM
Sbjct: 541 IKTLTAQQAADFTPKVFIDGDAWILNTGVPYSPGMM 571

BLAST of CSPI06G17480 vs. NCBI nr
Match: XP_004150503.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423.1 hypothetical protein Csa_021732 [Cucumis sativus])

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 575/576 (99.83%), Postives = 575/576 (99.83%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60
           MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60

Query: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120
           TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK
Sbjct: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180
           ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ
Sbjct: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180

Query: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDG 240
           TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDD 
Sbjct: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDD 240

Query: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300
           REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA
Sbjct: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300

Query: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360
           GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF
Sbjct: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360

Query: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420
           ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA
Sbjct: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420

Query: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480
           ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL
Sbjct: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480

Query: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540
           GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG
Sbjct: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540

Query: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 577
           IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM
Sbjct: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of CSPI06G17480 vs. NCBI nr
Match: XP_008458409.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo])

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 540/576 (93.75%), Postives = 564/576 (97.92%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60
           MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVG+NNAP+ EEHG+ + GS+TTPEI
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPY-EEHGEGDQGSKTTPEI 60

Query: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120
           +SSTKAIQAICQPTDYKETCEKSL AEAGNTTDPKELVKVGFKIATRSLNEAI+NSTTL+
Sbjct: 61  SSSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQ 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180
           EL+KDPRTNQALQNCRELLEYAIDDLN+SFERIGTFQMSKLD+FVADLKIWLSGALTYEQ
Sbjct: 121 ELSKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQ 180

Query: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDG 240
           TCLDGFENTTGDAG RMQEFLKSAQ+MTTNGLGIVNELSTILGSLQ+PGM+GRRLLEDD 
Sbjct: 181 TCLDGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPGMTGRRLLEDDD 240

Query: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300
            EMPSW+SDGKRRLMQAGAA MKPDL+VAQDGSGKYKTINAALADVPLKSNKTFVI+VKA
Sbjct: 241 HEMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKA 300

Query: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360
           GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF
Sbjct: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360

Query: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420
           ENTAG+AKHQAVALRVQSDRSIF+NCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA
Sbjct: 361 ENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420

Query: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480
           ATNFQNCKLVVRKP+DNQQCIVTAHGR+NRKEPTALIFQ CHFMGDPAYLPFK+INKAYL
Sbjct: 421 ATNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAYL 480

Query: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540
           GRPWK+YSRTIIIGS IDDIIQPEGWLPWMGDFGLNTLFYAEVQNKG GA E+KRVKWRG
Sbjct: 481 GRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWRG 540

Query: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 577
           IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM
Sbjct: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

BLAST of CSPI06G17480 vs. NCBI nr
Match: XP_038875843.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 1067.0 bits (2758), Expect = 5.7e-308
Identity = 528/576 (91.67%), Postives = 554/576 (96.18%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60
           MSNGFANAEGDKKKKK+AIISVSSLILVAMVVAVTVGVNNAP  EEHG D HG E T EI
Sbjct: 1   MSNGFANAEGDKKKKKMAIISVSSLILVAMVVAVTVGVNNAPK-EEHG-DGHGGE-THEI 60

Query: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120
           +S+TKAIQAICQPTDYK+ CEK+L AEAGNTTDPKEL KVG +IATR+LNEAI+NSTTL+
Sbjct: 61  SSTTKAIQAICQPTDYKDVCEKNLMAEAGNTTDPKELAKVGLRIATRNLNEAIRNSTTLQ 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180
           ELAKDPRTNQALQNCRELLEYAIDDLN+SFERIGTFQMSKLD+FVADLKIWLSGALTYEQ
Sbjct: 121 ELAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQ 180

Query: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDG 240
           TCLDGFENTTGDAGVRMQEFLKS+QQMTTNGL IVNELS +LGSLQ+PGM+GRRLLEDD 
Sbjct: 181 TCLDGFENTTGDAGVRMQEFLKSSQQMTTNGLAIVNELSGMLGSLQIPGMTGRRLLEDDA 240

Query: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300
            EMPSWVS+GKRRLMQA AA+MKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVI+VKA
Sbjct: 241 DEMPSWVSEGKRRLMQAAAASMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIYVKA 300

Query: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360
           GIYKEIVVIPK MTHLTMYGDGPTKT+VTGSLNFIDGIQTFKTATF+AIGANFYARDMGF
Sbjct: 301 GIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGIQTFKTATFAAIGANFYARDMGF 360

Query: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420
           ENTAGAAKHQAVALRVQSDRSIF+NC+IDGYQDTLYAHAHRQFYRDC ISGTIDFVFGNA
Sbjct: 361 ENTAGAAKHQAVALRVQSDRSIFYNCKIDGYQDTLYAHAHRQFYRDCIISGTIDFVFGNA 420

Query: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480
           AT FQNCKLVVRKP+DNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFK INKAYL
Sbjct: 421 ATIFQNCKLVVRKPMDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKNINKAYL 480

Query: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540
           GRPWK+YSRTIIIGS IDDIIQPEGWLPWMGDFGLNTLFYAEVQNKG GADESKRVKWRG
Sbjct: 481 GRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGADESKRVKWRG 540

Query: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 577
           I+HITPQHAAD+TPRRFIDGDAWIPAKGIPYSSGMM
Sbjct: 541 IRHITPQHAADYTPRRFIDGDAWIPAKGIPYSSGMM 573

BLAST of CSPI06G17480 vs. NCBI nr
Match: XP_023549205.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 957.2 bits (2473), Expect = 6.4e-275
Identity = 469/583 (80.45%), Postives = 516/583 (88.51%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPH-------GEEHGDDNHG 60
           MSNGF +AEGDKKKKKIA+ISVSSLILVAMVVAVTVGVNNAP        G   GD +  
Sbjct: 1   MSNGF-DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDH 60

Query: 61  SETTPEITSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAI 120
            +   +++SSTKAI+ ICQPTDYKETCE+SL  EAGNTTDPKELVK GFKIA   LN AI
Sbjct: 61  GDDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAI 120

Query: 121 KNSTTLKELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLS 180
           +NSTTL+ELA+DPR NQALQNCRELLEYAIDDL+ S+ RIGTF +SK ++FV DLKIWLS
Sbjct: 121 RNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLS 180

Query: 181 GALTYEQTCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGR 240
            ALTYEQTCLDGFENTTGD+G RM+EFLKSAQ+MTTNGLGIV+ELS ILG+L  P  +GR
Sbjct: 181 AALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSNILGTLHAPAATGR 240

Query: 241 RLLEDDGREMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKT 300
           RLL+ D   MP WVSDGKRRLMQ G  A+KPDLVVAQDGSGKYKTIN AL DVP+KSNKT
Sbjct: 241 RLLDTD---MPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVDVPMKSNKT 300

Query: 301 FVIHVKAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANF 360
           FVI+VKAGIYKEIVVIPKHMTHLTMYGDGPTK+VVTG+LNF+DGIQTFKTATF+AIGANF
Sbjct: 301 FVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANF 360

Query: 361 YARDMGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTI 420
           + RDMGF NTAG  KHQAVALRVQSDR+I +NCQIDGYQDTLYAHA+RQFYRDC ISGTI
Sbjct: 361 FVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTI 420

Query: 421 DFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFK 480
           DFVFGN+A  FQNCKLVVRKPLDNQQCIVTAHGRLNR+EPTALIFQ CHFMGDPAYLPFK
Sbjct: 421 DFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPAYLPFK 480

Query: 481 AINKAYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADES 540
            +NKAYLGRPWK+YSRTII+GS IDD+IQPEGWLPWMGDF LNTLFYAEVQN+G GAD+S
Sbjct: 481 KVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQS 540

Query: 541 KRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 577
            RVKWRGI+HITPQHAA +T RRFIDGDAWIP +G+PYSSGMM
Sbjct: 541 HRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of CSPI06G17480 vs. NCBI nr
Match: XP_023006255.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita maxima])

HSP 1 Score: 953.4 bits (2463), Expect = 9.2e-274
Identity = 472/604 (78.15%), Postives = 522/604 (86.42%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGE-----------EHGD 60
           MSNGF +AEGDKKKKKIA+ISVSSLILVAMVVAVTVGVNNAPH +           +HGD
Sbjct: 1   MSNGF-DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPHEDLDDGGGGGDLHDHGD 60

Query: 61  D-----NHG------------SETTPEITSSTKAIQAICQPTDYKETCEKSLEAEAGNTT 120
           D     +HG             +   +I+SSTKAI+ ICQPTDYKETCE+SL  EAGNTT
Sbjct: 61  DGGDVHDHGDYGGGGGDAHDHGDDGEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTT 120

Query: 121 DPKELVKVGFKIATRSLNEAIKNSTTLKELAKDPRTNQALQNCRELLEYAIDDLNQSFER 180
           D KELVK GFKIA   LN AI+NSTTL+ELAKDPR NQALQNCRELLEYAIDDL+ S+ R
Sbjct: 121 DLKELVKAGFKIAVEKLNAAIRNSTTLQELAKDPRANQALQNCRELLEYAIDDLHDSYHR 180

Query: 181 IGTFQMSKLDDFVADLKIWLSGALTYEQTCLDGFENTTGDAGVRMQEFLKSAQQMTTNGL 240
           IGTF +SK ++FV DLKIWLS ALTYEQTCLDGFENTTGD+G RM+EFLKSAQ+MTTNGL
Sbjct: 181 IGTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGL 240

Query: 241 GIVNELSTILGSLQLPGMSGRRLLEDDGREMPSWVSDGKRRLMQAGAAAMKPDLVVAQDG 300
           GIV+ELS ILG+L  P  +GRRLL+ D   MP WVSDGKRRLMQ    A+KPDLVVAQDG
Sbjct: 241 GIVSELSNILGTLHEPAATGRRLLDTD---MPLWVSDGKRRLMQLETGAIKPDLVVAQDG 300

Query: 301 SGKYKTINAALADVPLKSNKTFVIHVKAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSL 360
           SGKYKTIN AL DVP+KSNKTFVI+VKAGIYKEIVVIPKHMTHLTMYGDGPTK+VVTG+L
Sbjct: 301 SGKYKTINEALVDVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNL 360

Query: 361 NFIDGIQTFKTATFSAIGANFYARDMGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQ 420
           NF+DGIQTFKTATF+AIGANF+ RDMGF N+AG  KHQAVALR+QSDR+I +NCQIDG+Q
Sbjct: 361 NFVDGIQTFKTATFAAIGANFFVRDMGFVNSAGPTKHQAVALRIQSDRAIIYNCQIDGFQ 420

Query: 421 DTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKE 480
           DTLYAHA+RQFYRDCTISGTIDFVFGN+A  FQNCKLVVRKPLDNQQCIVTAHGRLNR+E
Sbjct: 421 DTLYAHANRQFYRDCTISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRRE 480

Query: 481 PTALIFQSCHFMGDPAYLPFKAINKAYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGD 540
           PTALIFQ CHFMGDPAYLPFK +NKAYLGRPWK+YSRTII+GS IDD+IQPEGWLPWMGD
Sbjct: 481 PTALIFQGCHFMGDPAYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGD 540

Query: 541 FGLNTLFYAEVQNKGAGADESKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYS 577
           FGLNTLFYAEVQN+G GAD+S RVKWRGI+HITPQHAA +T RRFIDGDAWIP +G+PYS
Sbjct: 541 FGLNTLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAASYTARRFIDGDAWIPVRGVPYS 600

BLAST of CSPI06G17480 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 658.3 bits (1697), Expect = 5.7e-189
Identity = 318/575 (55.30%), Postives = 420/575 (73.04%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60
           MS G+ + E  K+KK+  IIS+SS++L++MVVAVT+GV+       +  DN G E   EI
Sbjct: 1   MSYGY-DDEDAKRKKRYVIISISSVLLISMVVAVTIGVS------VNKSDNAGDE---EI 60

Query: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120
           T+S KAI+ +C PTDYKETCE +L  +A +T+DP ELVK  F    + +++  K S T+ 
Sbjct: 61  TTSVKAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMI 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180
           EL KDPR   AL  C+EL++YAI +L++SFE +G F+  K+D+ +  L+IWLS  +++EQ
Sbjct: 121 ELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQ 180

Query: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDG 240
           TCLDGF+ T G+AG  +++ LK+A Q+T NGL +V E+S  LG +Q+P M+ RRLL    
Sbjct: 181 TCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLL---S 240

Query: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300
           +E PSW+    RRL+ A  + +KPD+VVAQDGSG+YKTIN AL  VP K N TFV+H+K 
Sbjct: 241 QEFPSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKE 300

Query: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360
           GIYKE V + + MTHL   GDGP KTV++GS ++ DGI T+KTAT + +G +F A+++ F
Sbjct: 301 GIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAF 360

Query: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420
           ENTAGA KHQAVA+RV +D SIF+NC+ DGYQDTLYAH+HRQFYRDCTISGTIDF+FG+A
Sbjct: 361 ENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDA 420

Query: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480
           A  FQNC L+VRKPL NQ C +TAHGR + +E T  + Q C  +G+P YL  K  +K YL
Sbjct: 421 AAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYL 480

Query: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540
           GRPWKEYSRTII+ + I D + PEGW PW+G+FGLNTLFY+EVQN G GA  +KRV W G
Sbjct: 481 GRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPG 540

Query: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGM 576
           IK ++ +    FTP ++I GDAWIP KG+PY  G+
Sbjct: 541 IKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CSPI06G17480 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 656.4 bits (1692), Expect = 2.2e-188
Identity = 310/578 (53.63%), Postives = 424/578 (73.36%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60
           MS G+   +  K+K++  +I++SS++L++MVVAVTVGV+      +H  D+ G     E+
Sbjct: 1   MSYGY--DDESKRKRRYIVITISSVLLISMVVAVTVGVSL----NKHDGDSKGK---AEV 60

Query: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120
            +S KA++ +C PTDY++TCE +L     NTTDP ELVK  F +  + + +A K S T+ 
Sbjct: 61  NASVKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180
           EL KD RT  AL  C+EL++YA+D+L+ SFE +G F+   LD+ + +L+IWLS A+++E+
Sbjct: 121 ELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEE 180

Query: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDG 240
           TCL+GF+ T G+AG  M++ LK+A ++T NGL I++E+S  +G +Q+PG++ RRLL +  
Sbjct: 181 TCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEG- 240

Query: 241 REMPSWVSDGKRRLMQAGAA--AMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHV 300
              PSWV    R+L+QA AA   +KPD+VVAQDGSG+YKTIN AL  VP K N TFV+H+
Sbjct: 241 --FPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHI 300

Query: 301 KAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDM 360
           KAG+YKE V + K M+HL   GDGP KT+++G+ N+ DGI T++TAT + +G  F A+++
Sbjct: 301 KAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNI 360

Query: 361 GFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFG 420
           GFENTAGA KHQAVA+RVQSD SIFFNC+ DGYQDTLY H+HRQF+RDCTISGTIDF+FG
Sbjct: 361 GFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFG 420

Query: 421 NAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKA 480
           +AA  FQNC L+VRKPL NQ C +TAHGR + +E T  +FQ C   G+P YL  K  +KA
Sbjct: 421 DAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKA 480

Query: 481 YLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKW 540
           YLGRPWKEYSRTII+ + I D +QP+GW PW+GDFGL TLFY+EVQN G G+  + RV W
Sbjct: 481 YLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTW 540

Query: 541 RGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 577
            GIK ++ +    FTP ++I GD WIP KG+PY++G++
Sbjct: 541 AGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of CSPI06G17480 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 586.6 bits (1511), Expect = 2.1e-167
Identity = 281/574 (48.95%), Postives = 400/574 (69.69%), Query Frame = 0

Query: 9   EGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTP-EITSSTKAI 68
           +G+ KKKK  I  V + +LV MVVAV +  +         + +H  +  P +I ++T A+
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSR--------NTSHSEKIVPVQIKTATTAV 63

Query: 69  QAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTT--LKELAKD 128
           +A+C PTDYKETC  SL   + ++T P +L+K+GF +  RS+ ++IK ++     + A D
Sbjct: 64  EAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAAND 123

Query: 129 PRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTCLDG 188
             T  AL+ C +L+  A DDL +  +    F + +++DFV DL++WLSG++ Y+QTC+D 
Sbjct: 124 KDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDT 183

Query: 189 FENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSG------RRLLE-D 248
           FE T       MQ+  K+++++T+NGL ++  +S +LG   + G++G      R+LL  +
Sbjct: 184 FEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAE 243

Query: 249 DGREMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHV 308
           DG  +PSWV    RRLM A    +K ++VVA DGSG+YKTIN AL  VP  + K FVI++
Sbjct: 244 DG--IPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYI 303

Query: 309 KAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDG-IQTFKTATFSAIGANFYARD 368
           K G+Y E V + K MTH+T  GDGPTKT +TGSLN+  G ++T+ TAT +  G NF A++
Sbjct: 304 KQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKN 363

Query: 369 MGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVF 428
           +GFENTAG   HQAVALRV +D ++F+NCQIDGYQDTLY H+HRQF+RDCT+SGT+DF+F
Sbjct: 364 IGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIF 423

Query: 429 GNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINK 488
           G+     QNC +VVRKP+ +Q C++TA GR +++E T L+ Q+CH  G+PAY+P K+INK
Sbjct: 424 GDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINK 483

Query: 489 AYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVK 548
           AYLGRPWKE+SRTII+G+TIDD+I P GWLPW GDF LNTL+YAE +N G G+++++RVK
Sbjct: 484 AYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVK 543

Query: 549 WRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPY 572
           W GIK ++P+ A  FTP RF+ G+ WIP   +PY
Sbjct: 544 WPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of CSPI06G17480 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 545.4 bits (1404), Expect = 5.4e-155
Identity = 273/578 (47.23%), Postives = 388/578 (67.13%), Query Frame = 0

Query: 8   AEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEITSSTKAI 67
           ++GDKKKK I   SVS  +++ +V    V   ++P      D+NH       I  +TKA+
Sbjct: 3   SDGDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSP----KDDENH-------IRKTTKAV 62

Query: 68  QAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAI-KNSTTLKELA-KD 127
           QA+C PTD+K+TC  SL   + ++ DP +L+K+GFK+  +S+NE++ K S  +K  A K+
Sbjct: 63  QAVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKN 122

Query: 128 PRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTCLDG 187
           P    A + C +L+  AIDDL +  +    F + +++ FV DL++WLSG++ ++QTC+D 
Sbjct: 123 PEAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDS 182

Query: 188 FENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSG------RRLLEDD 247
           F     +    M +  K+++++++N L +V  +ST++ +  L G++G      R+LL  +
Sbjct: 183 FGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTE 242

Query: 248 GREMPSWVSDGKRRLMQA---GAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVI 307
              +P+WV    RRLM A   G   +K + VVAQDG+G++KTI  AL  VP  +   F+I
Sbjct: 243 D-SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFII 302

Query: 308 HVKAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNF-IDGIQTFKTATFSAIGANFYA 367
           H+K GIYKE V + K M H+T  GDGP KT++TGSLNF I  ++TF TAT +  G +F A
Sbjct: 303 HIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTA 362

Query: 368 RDMGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDF 427
           +++G ENTAG    QAVALRV +D ++F +CQIDG+QDTLY H+HRQFYRDCT+SGT+DF
Sbjct: 363 KNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDF 422

Query: 428 VFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAI 487
           +FG+A    QNCK+VVRKP   Q C+VTA GR N +E T L+   CH  GDPAY+P K++
Sbjct: 423 IFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSV 482

Query: 488 NKAYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKR 547
           NKAYLGRPWKE+SRTII+ +TIDD+I P GWLPW GDF L TL+YAE  N G G+++++R
Sbjct: 483 NKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQR 542

Query: 548 VKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSS 574
           VKW GIK +TPQ A  +T  RF+ GD WIP   +PY++
Sbjct: 543 VKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of CSPI06G17480 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 472.6 bits (1215), Expect = 4.4e-133
Identity = 269/706 (38.10%), Postives = 379/706 (53.68%), Query Frame = 0

Query: 16  KIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEITSSTKAIQAICQPTD 75
           K  ++ V++LI+ AMV+ V     N+P      ++ H  E+   IT +T +I  IC  TD
Sbjct: 3   KYVLLGVTALIM-AMVICVEANDGNSP--SRKMEEVH-RESKLMITKTTVSI--ICASTD 62

Query: 76  YKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAI-KNSTTLKELA-KDPRTNQALQ 135
           YK+ C  SL      + DP+ L++  F +A  S+   I +    LK  A  D  T +AL 
Sbjct: 63  YKQDCTTSLATV--RSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALN 122

Query: 136 NCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTCLDGFENTTGDA 195
            CREL++ AIDDL ++ ++   F  ++L DFV DL +WLSG++TY+QTC+DGFE    +A
Sbjct: 123 TCRELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEA 182

Query: 196 GVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLP------------------------- 255
            V M+  ++  Q +T+NGL I   L  +L + ++P                         
Sbjct: 183 AVMMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVG 242

Query: 256 ------------------------------------------------------------ 315
                                                                       
Sbjct: 243 SSQDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPK 302

Query: 316 --GMSGRRLLED------------------------------------------------ 375
               SG + L+D                                                
Sbjct: 303 KSAFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLD 362

Query: 376 --------DGREMPSWVSDGKRRLM--QAGAAAMKPDLVVAQDGSGKYKTINAALADVPL 435
                   +  E P WV+   RRL+  +     +K ++VVA+DGSGK KTI  ALA VP+
Sbjct: 363 SLKDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPM 422

Query: 436 KSNKTFVIHVKAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFI-DGIQTFKTATFS 495
           K+ K FVIH+K G+YKE V + K M H+   GDGPTKTV+TG + F+ D + T++TA+ +
Sbjct: 423 KNTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVA 482

Query: 496 AIGANFYARDMGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDC 555
             G  F A+D+GFENTAGAA+HQAVALRV +D ++FFNC ++GYQDTLY H HRQFYR+C
Sbjct: 483 VNGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNC 542

Query: 556 TISGTIDFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDP 574
            +SGTIDFVFG+A   FQNC+ V+R+P+++QQCIVTA GR +R+E T ++  +    GD 
Sbjct: 543 RVSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDA 602