CSPI06G12750 (gene) Cucumber (PI 183967) v1

BLAST of CSPI06G12750 vs. ExPASy Swiss-Prot
Match: Q9FKW0 (Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=RING1A PE=1 SV=2)

HSP 1 Score: 367.9 bits (943), Expect = 1.9e-100
Identity = 240/496 (48.39%), Postives = 338/496 (68.15%), Query Frame = 0

Query: 10  DSVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEE--EEEEQREEQLKEGQRVDEGEEEK 69
           D    E   D++  H +A++ + E     D EE++  ++ E+ EE+  E +  +E EEE+
Sbjct: 36  DETKEEKEGDEEVKHDEAEEDQ-EVVKPNDAEEDDDGDDAEEDEEEEVEAEEDEEAEEEE 95

Query: 70  QRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLGIIKKTRTVMECLH 129
           + ++ EE   +   E + SS S ++ EF+ ++L  IRKDVQCPICLGIIKKTRTVMECLH
Sbjct: 96  EEEEEEEEEEEDSKERSPSSISGDQSEFMEIDLGEIRKDVQCPICLGIIKKTRTVMECLH 155

Query: 130 RFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHE 189
           RFCRECIDKSMRLGNNECPACR HCASRRSLRDDP +DALIAAL+ +ID YEEEEL FHE
Sbjct: 156 RFCRECIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHE 215

Query: 190 EERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGV-----ILTRSRRNHRNVHLRRQN 249
           +E  RNKQIQ SIAQI QRQSEAL K+R LGK+ A +     I + SRR   + ++ +QN
Sbjct: 216 DEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQN 275

Query: 250 GRGDEVSGYEDNDDDDDNNEGKD-SSSADERFTEVRQRRKKRHPTVRSSQ-PSSSIANID 309
                   +ED+D+DD+NN G+D  SS+DER TEVRQ+++++  T RS+Q PSSS AN +
Sbjct: 276 AS----EAHEDDDNDDNNNRGRDKDSSSDERGTEVRQKKRRKRSTSRSTQHPSSSGANKN 335

Query: 310 SGDGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHG-SAGGSGNKSSRSSRL 369
           +G+ CA++D ++ R+ + +SPGLV N E+L WGRGG RSN+RHG +  G  +KS R++R+
Sbjct: 336 NGN-CADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARV 395

Query: 370 MKLAKYLR-GLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVSRKTP 429
            KL +YLR  ++ ++ ELD+H+ L+S+D +  P L QP+L CRP+L VK LRE+V+ +  
Sbjct: 396 NKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIH 455

Query: 430 LQADDVEILSLKGR-PRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGLQSD 489
           L+ ++VE+L  + R    +  ++ P+ + +    S      K E+ SLE    L+ L+ D
Sbjct: 456 LKTEEVELLVTRRRVGEDAAIENLPAVTPASAAAS------KDEMLSLEDNETLSRLKID 515

Query: 490 CTYSRDL-LILGYRRK 493
              S +  LI+ YR+K
Sbjct: 516 FISSHEQHLIIAYRKK 519

BLAST of CSPI06G12750 vs. ExPASy Swiss-Prot
Match: Q0WX00 (Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=RING1B PE=1 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 8.7e-90
Identity = 231/475 (48.63%), Postives = 313/475 (65.89%), Query Frame = 0

Query: 25  QDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRVDEGEEEKQRQQLEEATGDRD--SE 84
           Q  + S  ER     +E+EE+ ++  +E+ + G   D+ + ++   + EEA G+ +   E
Sbjct: 16  QLGRNSEAERFNPEAVEKEEDPDKM-DEKDESGDEEDDVKRDQVEAEDEEALGEEEDSKE 75

Query: 85  GTQSSSSEE--KPEFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRL 144
            +QSSS+ E  + E++ V+L+ I KDVQC ICLGII+KTRTVMECLHRFCRECIDKSMRL
Sbjct: 76  RSQSSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRL 135

Query: 145 GNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEERNRNKQIQESI 204
           GNNECP CR HCASRRSLRDDPN+DALIAAL+ +IDK+EEEEL F +++  RNKQIQ SI
Sbjct: 136 GNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASI 195

Query: 205 AQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRRQNGRGDEVSGYEDNDDDDDN 264
           AQ+ QRQS+AL K++ +GK TA  IL+RSRR+      RR+N R  E    E NDDDD N
Sbjct: 196 AQVSQRQSKALVKRKSVGKGTA--ILSRSRRSGGGSR-RRRNCRNIEQDTSEANDDDDQN 255

Query: 265 NEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANIDSGDGCAESDLDLS--RENRT 324
             GKDSSS DE      ++RKKR  T    QPSSS AN  + D CA +  + +  R++R 
Sbjct: 256 KRGKDSSS-DE---PCERQRKKRSAT----QPSSSNAN--NNDNCAGNGTEQTHQRDSRV 315

Query: 325 VSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLMKLAKYLRGLEENNNELDV 384
           +SP LV N+E++ WGRGG RSN+R G+    G  S R++RL +L +YL  LE N+ ELD+
Sbjct: 316 ISPVLVWNSELIAWGRGGTRSNTRQGN-NNQGAISKRNARLKRLVEYLGSLEGNSVELDI 375

Query: 385 HLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVSRKTPLQADDVEILSLKGRPRTSNE 444
           HL L+S+D E   +L +P+L  RP+L VK LREYV+R   L+A++VE+L           
Sbjct: 376 HLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELL----------- 435

Query: 445 QSTPSASISIDGMSLVFDPLKYE-LQSLEGEIFLAGLQSDCTYSRDLLILGYRRK 493
                  +S DG +++ +    E +QSL+ +  +A L+ DC  S   +I+ YRRK
Sbjct: 436 -------VSKDGDTVIGNKTSTEKMQSLQDDETVAKLKVDCISSNGYMIVVYRRK 457

BLAST of CSPI06G12750 vs. ExPASy Swiss-Prot
Match: Q66J69 (E3 ubiquitin-protein ligase RING2-A OS=Xenopus laevis OX=8355 GN=rnf2-a PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.9e-20
Identity = 44/87 (50.57%), Postives = 58/87 (66.67%), Query Frame = 0

Query: 96  IFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 155
           I V    +  ++ CPICL ++K T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 35  IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 94

Query: 156 RSLRDDPNYDALIAALYPDIDKYEEEE 183
           RSLR DPN+DALI+ +YP  D+YE  +
Sbjct: 95  RSLRPDPNFDALISKIYPSRDEYEAHQ 121

BLAST of CSPI06G12750 vs. ExPASy Swiss-Prot
Match: Q7ZWM8 (E3 ubiquitin-protein ligase RING2-B OS=Xenopus laevis OX=8355 GN=rnf2-b PE=2 SV=2)

HSP 1 Score: 102.1 bits (253), Expect = 1.9e-20
Identity = 44/87 (50.57%), Postives = 58/87 (66.67%), Query Frame = 0

Query: 96  IFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 155
           I V    +  ++ CPICL ++K T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 34  IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 93

Query: 156 RSLRDDPNYDALIAALYPDIDKYEEEE 183
           RSLR DPN+DALI+ +YP  D+YE  +
Sbjct: 94  RSLRPDPNFDALISKIYPSRDEYEAHQ 120

BLAST of CSPI06G12750 vs. ExPASy Swiss-Prot
Match: Q99496 (E3 ubiquitin-protein ligase RING2 OS=Homo sapiens OX=9606 GN=RNF2 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 3.4e-17
Identity = 57/179 (31.84%), Postives = 90/179 (50.28%), Query Frame = 0

Query: 96  IFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 155
           I V    +  ++ CPICL ++K T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 38  IVVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCADCIITALRSGNKECPTCRKKLVSK 97

Query: 156 RSLRDDPNYDALIAALYPDIDKYE--EEELTFHEEERNRNKQIQESIAQIFQRQSEALSK 215
           RSLR DPN+DALI+ +YP  D+YE  +E +     + N  + +  SI +           
Sbjct: 98  RSLRPDPNFDALISKIYPSRDEYEAHQERVLARINKHNNQQALSHSIEE----------- 157

Query: 216 KRILGKDTAGVILTRSRRNHRNVHLRRQNGRGDEVSGYEDNDDDDDNNEGKDSSSADER 273
                    G+ +    R  R    + +NG G E +G   +  +   +  +++  +++R
Sbjct: 158 ---------GLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKR 196

BLAST of CSPI06G12750 vs. ExPASy TrEMBL
Match: A0A0A0KB89 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G155540 PE=4 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 9.1e-268
Identity = 493/495 (99.60%), Postives = 495/495 (100.00%), Query Frame = 0

Query: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRV 60
           MPAHKRSWSD+VHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRV
Sbjct: 1   MPAHKRSWSDTVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRV 60

Query: 61  DEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLGIIKKTR 120
           DEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLGIIKKTR
Sbjct: 61  DEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLGIIKKTR 120

Query: 121 TVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEE 180
           TVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEE
Sbjct: 121 TVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEE 180

Query: 181 EELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRR 240
           EELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRR
Sbjct: 181 EELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRR 240

Query: 241 QNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANID 300
           QNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANID
Sbjct: 241 QNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANID 300

Query: 301 SGDGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLM 360
           SGDGCAESDLD+SRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLM
Sbjct: 301 SGDGCAESDLDMSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLM 360

Query: 361 KLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVSRKTPLQ 420
           KLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVSRKTPLQ
Sbjct: 361 KLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVSRKTPLQ 420

Query: 421 ADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGLQSDCTY 480
           ADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGLQSDCTY
Sbjct: 421 ADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGLQSDCTY 480

Query: 481 SRDLLILGYRRKGRS 496
           SRDLLILGYRRKGRS
Sbjct: 481 SRDLLILGYRRKGRS 495

BLAST of CSPI06G12750 vs. ExPASy TrEMBL
Match: A0A1S3BJA1 (putative E3 ubiquitin-protein ligase RING1b isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490502 PE=4 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.8e-255
Identity = 476/501 (95.01%), Postives = 486/501 (97.01%), Query Frame = 0

Query: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQR- 60
           MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERET T+IEEEEEEEEQREEQLKEGQR 
Sbjct: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETNTEIEEEEEEEEQREEQLKEGQRG 60

Query: 61  -----VDEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLG 120
                 DEGEEEKQRQQLEEATGDRDSEG+QSSSSEEKPEFIFVELSGIRKDVQCPICLG
Sbjct: 61  DEGREEDEGEEEKQRQQLEEATGDRDSEGSQSSSSEEKPEFIFVELSGIRKDVQCPICLG 120

Query: 121 IIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPD 180
           IIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPD
Sbjct: 121 IIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPD 180

Query: 181 IDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHR 240
           IDKYEEEELTFHEE+RNRNKQIQESIAQIFQRQSEALSKKR+LGKDTAGVILTRSRRNHR
Sbjct: 181 IDKYEEEELTFHEEDRNRNKQIQESIAQIFQRQSEALSKKRMLGKDTAGVILTRSRRNHR 240

Query: 241 NVHLRRQNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSS 300
           NVHLRRQNGRG+EVSGYEDN+DDDDNNEGKDSSSADERFTEVRQRRKKRHPT+RSSQPSS
Sbjct: 241 NVHLRRQNGRGEEVSGYEDNEDDDDNNEGKDSSSADERFTEVRQRRKKRHPTLRSSQPSS 300

Query: 301 SIANIDSGDGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSS 360
           SIANIDSGDGCAESDLD+SRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSS
Sbjct: 301 SIANIDSGDGCAESDLDMSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSS 360

Query: 361 RSSRLMKLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVS 420
           RSSRLMKL KYLRGLEENNNELDVHLLLISVDKESTPSLQQPHL+CRPSLTVKHLREYVS
Sbjct: 361 RSSRLMKLVKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVS 420

Query: 421 RKTPLQADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGL 480
           RKT LQADDVEILSLKGRPRTSNEQST SASISIDGMSLVFDP KYELQSLEGE  LAGL
Sbjct: 421 RKTSLQADDVEILSLKGRPRTSNEQSTRSASISIDGMSLVFDPFKYELQSLEGEKNLAGL 480

Query: 481 QSDCTYSRDLLILGYRRKGRS 496
           QSDCTYSRD LILGYRR+GRS
Sbjct: 481 QSDCTYSRDHLILGYRRRGRS 501

BLAST of CSPI06G12750 vs. ExPASy TrEMBL
Match: A0A1S3BIP1 (putative E3 ubiquitin-protein ligase RING1b isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490502 PE=4 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 4.4e-254
Identity = 476/502 (94.82%), Postives = 486/502 (96.81%), Query Frame = 0

Query: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQR- 60
           MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERET T+IEEEEEEEEQREEQLKEGQR 
Sbjct: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETNTEIEEEEEEEEQREEQLKEGQRG 60

Query: 61  -----VDEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLG 120
                 DEGEEEKQRQQLEEATGDRDSEG+QSSSSEEKPEFIFVELSGIRKDVQCPICLG
Sbjct: 61  DEGREEDEGEEEKQRQQLEEATGDRDSEGSQSSSSEEKPEFIFVELSGIRKDVQCPICLG 120

Query: 121 IIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPD 180
           IIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPD
Sbjct: 121 IIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPD 180

Query: 181 IDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHR 240
           IDKYEEEELTFHEE+RNRNKQIQESIAQIFQRQSEALSKKR+LGKDTAGVILTRSRRNHR
Sbjct: 181 IDKYEEEELTFHEEDRNRNKQIQESIAQIFQRQSEALSKKRMLGKDTAGVILTRSRRNHR 240

Query: 241 NVHLRRQNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSS 300
           NVHLRRQNGRG+EVSGYEDN+DDDDNNEGKDSSSADERFTEVRQRRKKRHPT+RSSQPSS
Sbjct: 241 NVHLRRQNGRGEEVSGYEDNEDDDDNNEGKDSSSADERFTEVRQRRKKRHPTLRSSQPSS 300

Query: 301 SIANIDSGDGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSS 360
           SIANIDSGDGCAESDLD+SRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSS
Sbjct: 301 SIANIDSGDGCAESDLDMSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSS 360

Query: 361 RSSRLMKLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLRE-YV 420
           RSSRLMKL KYLRGLEENNNELDVHLLLISVDKESTPSLQQPHL+CRPSLTVKHLRE YV
Sbjct: 361 RSSRLMKLVKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREQYV 420

Query: 421 SRKTPLQADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAG 480
           SRKT LQADDVEILSLKGRPRTSNEQST SASISIDGMSLVFDP KYELQSLEGE  LAG
Sbjct: 421 SRKTSLQADDVEILSLKGRPRTSNEQSTRSASISIDGMSLVFDPFKYELQSLEGEKNLAG 480

Query: 481 LQSDCTYSRDLLILGYRRKGRS 496
           LQSDCTYSRD LILGYRR+GRS
Sbjct: 481 LQSDCTYSRDHLILGYRRRGRS 502

BLAST of CSPI06G12750 vs. ExPASy TrEMBL
Match: A0A6J1FY08 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448324 PE=4 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 2.1e-232
Identity = 442/504 (87.70%), Postives = 463/504 (91.87%), Query Frame = 0

Query: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEE-EEEQREEQLKEGQR 60
           MPAHKRSWSDSV+GE+  DQQFSHQDAKQSR ERE KT+ EEEEE EEE++ E LKEG+R
Sbjct: 1   MPAHKRSWSDSVNGEESGDQQFSHQDAKQSRMEREPKTETEEEEEVEEEEQREHLKEGER 60

Query: 61  ------VDEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICL 120
                  DEGEEEKQ  QLEEA G RDSEG+QSSSSEEKPEFIFVELSGIRKDVQCPICL
Sbjct: 61  EDEGREEDEGEEEKQHNQLEEAAGARDSEGSQSSSSEEKPEFIFVELSGIRKDVQCPICL 120

Query: 121 GIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYP 180
           GIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYP
Sbjct: 121 GIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYP 180

Query: 181 DIDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNH 240
           DIDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTA V LTRSRRNH
Sbjct: 181 DIDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAAVFLTRSRRNH 240

Query: 241 RNVHLRRQNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPS 300
           RN HLRRQNGRGDEV GYEDN+D+DDNNEGKDSSSADERFTEVRQRRKKRHPTV SSQPS
Sbjct: 241 RNAHLRRQNGRGDEVLGYEDNEDEDDNNEGKDSSSADERFTEVRQRRKKRHPTVPSSQPS 300

Query: 301 SSIANIDSG--DGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGN 360
           SSIAN+DSG  DGCAES+LD+SRENRTVSPGLVLN+EMLGWGRGGVRSN+RH SAGGS N
Sbjct: 301 SSIANVDSGCTDGCAESELDMSRENRTVSPGLVLNSEMLGWGRGGVRSNARHSSAGGSSN 360

Query: 361 KSSRSSRLMKLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLRE 420
           KSSRSSRL KL  YLRGLEENNNELDVHLLLISVDKES PSLQQPHL+CRPSLTV+HLRE
Sbjct: 361 KSSRSSRLTKLVNYLRGLEENNNELDVHLLLISVDKESAPSLQQPHLHCRPSLTVEHLRE 420

Query: 421 YVSRKTPLQADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFL 480
           YVSRKTPLQADDVEILS+K R  TS+ Q+TPSASISIDG SLVFDPLKYELQ+LEGE  L
Sbjct: 421 YVSRKTPLQADDVEILSMKARHSTSDVQTTPSASISIDGTSLVFDPLKYELQTLEGEETL 480

Query: 481 AGLQSDCTYSRDLLILGYRRKGRS 496
           AGLQ+DC YSRD LILGYRRKGRS
Sbjct: 481 AGLQADCIYSRDHLILGYRRKGRS 504

BLAST of CSPI06G12750 vs. ExPASy TrEMBL
Match: A0A6J1J6A5 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483842 PE=4 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 4.7e-232
Identity = 441/504 (87.50%), Postives = 463/504 (91.87%), Query Frame = 0

Query: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEE-EEEQREEQLKEGQR 60
           MPAHKRSWSDSV+GE+  DQQFSHQDAKQSR ERE KT+ EEEEE EEE++ E LKEG+R
Sbjct: 1   MPAHKRSWSDSVNGEESGDQQFSHQDAKQSRMEREPKTETEEEEEVEEEEQREHLKEGER 60

Query: 61  ------VDEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICL 120
                  DEGEEEKQ  QLEEA G RDSEG+QSSSSEEKPEFIFVELSGIRKDVQCPICL
Sbjct: 61  EDEEREEDEGEEEKQHNQLEEAAGARDSEGSQSSSSEEKPEFIFVELSGIRKDVQCPICL 120

Query: 121 GIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYP 180
           GIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALI+ALYP
Sbjct: 121 GIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALISALYP 180

Query: 181 DIDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNH 240
           DIDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTA V LTRSRRNH
Sbjct: 181 DIDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAAVFLTRSRRNH 240

Query: 241 RNVHLRRQNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPS 300
           RN HLRRQNGRGDEV GYEDN+D+DDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPS
Sbjct: 241 RNAHLRRQNGRGDEVLGYEDNEDEDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPS 300

Query: 301 SSIANIDSG--DGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGN 360
           SSIAN+DSG  DGCAES+LD+SRENRTVSPGLVLN+EMLGWGRGGVRSN+RH SAGGS N
Sbjct: 301 SSIANVDSGCTDGCAESELDMSRENRTVSPGLVLNSEMLGWGRGGVRSNARHSSAGGSSN 360

Query: 361 KSSRSSRLMKLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLRE 420
           KSSRSSRL KL  YLRGLEENNNELDVHLLLISVDKES PSLQQPHL+CRPSLTV+HLRE
Sbjct: 361 KSSRSSRLTKLVNYLRGLEENNNELDVHLLLISVDKESAPSLQQPHLHCRPSLTVEHLRE 420

Query: 421 YVSRKTPLQADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFL 480
           YVSRKTPLQADDVEILS+K R  TS+ Q+TPSASISIDG SLVFDPLKYELQ+LEGE  L
Sbjct: 421 YVSRKTPLQADDVEILSMKARHSTSDVQTTPSASISIDGTSLVFDPLKYELQTLEGEETL 480

Query: 481 AGLQSDCTYSRDLLILGYRRKGRS 496
           AGLQ+DC YSRD LILGYRRKG S
Sbjct: 481 AGLQADCIYSRDHLILGYRRKGLS 504

BLAST of CSPI06G12750 vs. NCBI nr
Match: XP_004149271.1 (putative E3 ubiquitin-protein ligase RING1a isoform X2 [Cucumis sativus])

HSP 1 Score: 932.2 bits (2408), Expect = 1.9e-267
Identity = 493/495 (99.60%), Postives = 495/495 (100.00%), Query Frame = 0

Query: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRV 60
           MPAHKRSWSD+VHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRV
Sbjct: 1   MPAHKRSWSDTVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRV 60

Query: 61  DEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLGIIKKTR 120
           DEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLGIIKKTR
Sbjct: 61  DEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLGIIKKTR 120

Query: 121 TVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEE 180
           TVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEE
Sbjct: 121 TVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEE 180

Query: 181 EELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRR 240
           EELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRR
Sbjct: 181 EELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRR 240

Query: 241 QNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANID 300
           QNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANID
Sbjct: 241 QNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANID 300

Query: 301 SGDGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLM 360
           SGDGCAESDLD+SRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLM
Sbjct: 301 SGDGCAESDLDMSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLM 360

Query: 361 KLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVSRKTPLQ 420
           KLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVSRKTPLQ
Sbjct: 361 KLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVSRKTPLQ 420

Query: 421 ADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGLQSDCTY 480
           ADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGLQSDCTY
Sbjct: 421 ADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGLQSDCTY 480

Query: 481 SRDLLILGYRRKGRS 496
           SRDLLILGYRRKGRS
Sbjct: 481 SRDLLILGYRRKGRS 495

BLAST of CSPI06G12750 vs. NCBI nr
Match: XP_011657079.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucumis sativus])

HSP 1 Score: 927.5 bits (2396), Expect = 4.7e-266
Identity = 493/496 (99.40%), Postives = 495/496 (99.80%), Query Frame = 0

Query: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRV 60
           MPAHKRSWSD+VHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRV
Sbjct: 1   MPAHKRSWSDTVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRV 60

Query: 61  DEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLGIIKKTR 120
           DEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLGIIKKTR
Sbjct: 61  DEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLGIIKKTR 120

Query: 121 TVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEE 180
           TVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEE
Sbjct: 121 TVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEE 180

Query: 181 EELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRR 240
           EELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRR
Sbjct: 181 EELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRR 240

Query: 241 QNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANID 300
           QNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANID
Sbjct: 241 QNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANID 300

Query: 301 SGDGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLM 360
           SGDGCAESDLD+SRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLM
Sbjct: 301 SGDGCAESDLDMSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLM 360

Query: 361 KLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLRE-YVSRKTPL 420
           KLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLRE YVSRKTPL
Sbjct: 361 KLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLREQYVSRKTPL 420

Query: 421 QADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGLQSDCT 480
           QADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGLQSDCT
Sbjct: 421 QADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGLQSDCT 480

Query: 481 YSRDLLILGYRRKGRS 496
           YSRDLLILGYRRKGRS
Sbjct: 481 YSRDLLILGYRRKGRS 496

BLAST of CSPI06G12750 vs. NCBI nr
Match: XP_008448258.1 (PREDICTED: putative E3 ubiquitin-protein ligase RING1b isoform X2 [Cucumis melo])

HSP 1 Score: 891.3 bits (2302), Expect = 3.7e-255
Identity = 476/501 (95.01%), Postives = 486/501 (97.01%), Query Frame = 0

Query: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQR- 60
           MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERET T+IEEEEEEEEQREEQLKEGQR 
Sbjct: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETNTEIEEEEEEEEQREEQLKEGQRG 60

Query: 61  -----VDEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLG 120
                 DEGEEEKQRQQLEEATGDRDSEG+QSSSSEEKPEFIFVELSGIRKDVQCPICLG
Sbjct: 61  DEGREEDEGEEEKQRQQLEEATGDRDSEGSQSSSSEEKPEFIFVELSGIRKDVQCPICLG 120

Query: 121 IIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPD 180
           IIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPD
Sbjct: 121 IIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPD 180

Query: 181 IDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHR 240
           IDKYEEEELTFHEE+RNRNKQIQESIAQIFQRQSEALSKKR+LGKDTAGVILTRSRRNHR
Sbjct: 181 IDKYEEEELTFHEEDRNRNKQIQESIAQIFQRQSEALSKKRMLGKDTAGVILTRSRRNHR 240

Query: 241 NVHLRRQNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSS 300
           NVHLRRQNGRG+EVSGYEDN+DDDDNNEGKDSSSADERFTEVRQRRKKRHPT+RSSQPSS
Sbjct: 241 NVHLRRQNGRGEEVSGYEDNEDDDDNNEGKDSSSADERFTEVRQRRKKRHPTLRSSQPSS 300

Query: 301 SIANIDSGDGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSS 360
           SIANIDSGDGCAESDLD+SRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSS
Sbjct: 301 SIANIDSGDGCAESDLDMSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSS 360

Query: 361 RSSRLMKLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVS 420
           RSSRLMKL KYLRGLEENNNELDVHLLLISVDKESTPSLQQPHL+CRPSLTVKHLREYVS
Sbjct: 361 RSSRLMKLVKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVS 420

Query: 421 RKTPLQADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGL 480
           RKT LQADDVEILSLKGRPRTSNEQST SASISIDGMSLVFDP KYELQSLEGE  LAGL
Sbjct: 421 RKTSLQADDVEILSLKGRPRTSNEQSTRSASISIDGMSLVFDPFKYELQSLEGEKNLAGL 480

Query: 481 QSDCTYSRDLLILGYRRKGRS 496
           QSDCTYSRD LILGYRR+GRS
Sbjct: 481 QSDCTYSRDHLILGYRRRGRS 501

BLAST of CSPI06G12750 vs. NCBI nr
Match: XP_008448257.1 (PREDICTED: putative E3 ubiquitin-protein ligase RING1b isoform X1 [Cucumis melo])

HSP 1 Score: 886.7 bits (2290), Expect = 9.1e-254
Identity = 476/502 (94.82%), Postives = 486/502 (96.81%), Query Frame = 0

Query: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQR- 60
           MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERET T+IEEEEEEEEQREEQLKEGQR 
Sbjct: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETNTEIEEEEEEEEQREEQLKEGQRG 60

Query: 61  -----VDEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLG 120
                 DEGEEEKQRQQLEEATGDRDSEG+QSSSSEEKPEFIFVELSGIRKDVQCPICLG
Sbjct: 61  DEGREEDEGEEEKQRQQLEEATGDRDSEGSQSSSSEEKPEFIFVELSGIRKDVQCPICLG 120

Query: 121 IIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPD 180
           IIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPD
Sbjct: 121 IIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPD 180

Query: 181 IDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHR 240
           IDKYEEEELTFHEE+RNRNKQIQESIAQIFQRQSEALSKKR+LGKDTAGVILTRSRRNHR
Sbjct: 181 IDKYEEEELTFHEEDRNRNKQIQESIAQIFQRQSEALSKKRMLGKDTAGVILTRSRRNHR 240

Query: 241 NVHLRRQNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSS 300
           NVHLRRQNGRG+EVSGYEDN+DDDDNNEGKDSSSADERFTEVRQRRKKRHPT+RSSQPSS
Sbjct: 241 NVHLRRQNGRGEEVSGYEDNEDDDDNNEGKDSSSADERFTEVRQRRKKRHPTLRSSQPSS 300

Query: 301 SIANIDSGDGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSS 360
           SIANIDSGDGCAESDLD+SRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSS
Sbjct: 301 SIANIDSGDGCAESDLDMSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSS 360

Query: 361 RSSRLMKLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLRE-YV 420
           RSSRLMKL KYLRGLEENNNELDVHLLLISVDKESTPSLQQPHL+CRPSLTVKHLRE YV
Sbjct: 361 RSSRLMKLVKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREQYV 420

Query: 421 SRKTPLQADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAG 480
           SRKT LQADDVEILSLKGRPRTSNEQST SASISIDGMSLVFDP KYELQSLEGE  LAG
Sbjct: 421 SRKTSLQADDVEILSLKGRPRTSNEQSTRSASISIDGMSLVFDPFKYELQSLEGEKNLAG 480

Query: 481 LQSDCTYSRDLLILGYRRKGRS 496
           LQSDCTYSRD LILGYRR+GRS
Sbjct: 481 LQSDCTYSRDHLILGYRRRGRS 502

BLAST of CSPI06G12750 vs. NCBI nr
Match: XP_038901730.1 (putative E3 ubiquitin-protein ligase RING1a [Benincasa hispida])

HSP 1 Score: 841.6 bits (2173), Expect = 3.4e-240
Identity = 453/504 (89.88%), Postives = 474/504 (94.05%), Query Frame = 0

Query: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERETKTDI--EEEEEEEEQREEQLKEGQ 60
           MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTERE KT+I  EEEEEEEE++ EQLKEG+
Sbjct: 1   MPAHKRSWSDSVHGEDPRDQQFSHQDAKQSRTEREPKTEIEGEEEEEEEEEQREQLKEGE 60

Query: 61  -----RVDEGEEEKQRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICL 120
                R DEGEEEKQ ++LEEA G RDSEG+QSSSSEEKPEFIFVELSGIRKDVQCPICL
Sbjct: 61  REDEGREDEGEEEKQHKKLEEANGARDSEGSQSSSSEEKPEFIFVELSGIRKDVQCPICL 120

Query: 121 GIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYP 180
           GIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYP
Sbjct: 121 GIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYP 180

Query: 181 DIDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNH 240
           DIDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNH
Sbjct: 181 DIDKYEEEELTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNH 240

Query: 241 RNVHLRRQNGRGDEVSGYEDNDDDDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPS 300
           RN+HLRRQNGRGD+VSGYEDN+D+DDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPS
Sbjct: 241 RNLHLRRQNGRGDDVSGYEDNEDEDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPS 300

Query: 301 SSIANIDSG--DGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGN 360
           SSIA++DSG  DGCAESDLD+SR+NRTVSPGLVLN+EMLGWGRGGVRSNSRHGSAGGS N
Sbjct: 301 SSIAHVDSGCTDGCAESDLDMSRDNRTVSPGLVLNSEMLGWGRGGVRSNSRHGSAGGSSN 360

Query: 361 KSSRSSRLMKLAKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLRE 420
           KSSRSSRLMKL  YLRGL+ENNNELDVHLLLISVDKESTPSL+QPHL+CRPSLTVKHLRE
Sbjct: 361 KSSRSSRLMKLVNYLRGLDENNNELDVHLLLISVDKESTPSLKQPHLHCRPSLTVKHLRE 420

Query: 421 YVSRKTPLQADDVEILSLKGRPRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFL 480
           YVSRKTPLQADDVEILS+KGR  TS +QSTP  SISIDGMSLVFDPLKYELQ LEGE  L
Sbjct: 421 YVSRKTPLQADDVEILSVKGRHSTSKDQSTPRTSISIDGMSLVFDPLKYELQILEGEETL 480

Query: 481 AGLQSDCTYSRDLLILGYRRKGRS 496
           AGLQ DC YS D LILGYRRKGRS
Sbjct: 481 AGLQPDCIYSTDHLILGYRRKGRS 504

BLAST of CSPI06G12750 vs. TAIR 10
Match: AT5G44280.1 (RING 1A )

HSP 1 Score: 367.9 bits (943), Expect = 1.3e-101
Identity = 240/496 (48.39%), Postives = 338/496 (68.15%), Query Frame = 0

Query: 10  DSVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEE--EEEEQREEQLKEGQRVDEGEEEK 69
           D    E   D++  H +A++ + E     D EE++  ++ E+ EE+  E +  +E EEE+
Sbjct: 36  DETKEEKEGDEEVKHDEAEEDQ-EVVKPNDAEEDDDGDDAEEDEEEEVEAEEDEEAEEEE 95

Query: 70  QRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLGIIKKTRTVMECLH 129
           + ++ EE   +   E + SS S ++ EF+ ++L  IRKDVQCPICLGIIKKTRTVMECLH
Sbjct: 96  EEEEEEEEEEEDSKERSPSSISGDQSEFMEIDLGEIRKDVQCPICLGIIKKTRTVMECLH 155

Query: 130 RFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHE 189
           RFCRECIDKSMRLGNNECPACR HCASRRSLRDDP +DALIAAL+ +ID YEEEEL FHE
Sbjct: 156 RFCRECIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHE 215

Query: 190 EERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGV-----ILTRSRRNHRNVHLRRQN 249
           +E  RNKQIQ SIAQI QRQSEAL K+R LGK+ A +     I + SRR   + ++ +QN
Sbjct: 216 DEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQN 275

Query: 250 GRGDEVSGYEDNDDDDDNNEGKD-SSSADERFTEVRQRRKKRHPTVRSSQ-PSSSIANID 309
                   +ED+D+DD+NN G+D  SS+DER TEVRQ+++++  T RS+Q PSSS AN +
Sbjct: 276 AS----EAHEDDDNDDNNNRGRDKDSSSDERGTEVRQKKRRKRSTSRSTQHPSSSGANKN 335

Query: 310 SGDGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHG-SAGGSGNKSSRSSRL 369
           +G+ CA++D ++ R+ + +SPGLV N E+L WGRGG RSN+RHG +  G  +KS R++R+
Sbjct: 336 NGN-CADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARV 395

Query: 370 MKLAKYLR-GLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVSRKTP 429
            KL +YLR  ++ ++ ELD+H+ L+S+D +  P L QP+L CRP+L VK LRE+V+ +  
Sbjct: 396 NKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIH 455

Query: 430 LQADDVEILSLKGR-PRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAGLQSD 489
           L+ ++VE+L  + R    +  ++ P+ + +    S      K E+ SLE    L+ L+ D
Sbjct: 456 LKTEEVELLVTRRRVGEDAAIENLPAVTPASAAAS------KDEMLSLEDNETLSRLKID 515

Query: 490 CTYSRDL-LILGYRRK 493
              S +  LI+ YR+K
Sbjct: 516 FISSHEQHLIIAYRKK 519

BLAST of CSPI06G12750 vs. TAIR 10
Match: AT1G03770.1 (RING 1B )

HSP 1 Score: 332.4 bits (851), Expect = 6.2e-91
Identity = 231/475 (48.63%), Postives = 313/475 (65.89%), Query Frame = 0

Query: 25  QDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRVDEGEEEKQRQQLEEATGDRD--SE 84
           Q  + S  ER     +E+EE+ ++  +E+ + G   D+ + ++   + EEA G+ +   E
Sbjct: 16  QLGRNSEAERFNPEAVEKEEDPDKM-DEKDESGDEEDDVKRDQVEAEDEEALGEEEDSKE 75

Query: 85  GTQSSSSEE--KPEFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRL 144
            +QSSS+ E  + E++ V+L+ I KDVQC ICLGII+KTRTVMECLHRFCRECIDKSMRL
Sbjct: 76  RSQSSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRL 135

Query: 145 GNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEERNRNKQIQESI 204
           GNNECP CR HCASRRSLRDDPN+DALIAAL+ +IDK+EEEEL F +++  RNKQIQ SI
Sbjct: 136 GNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASI 195

Query: 205 AQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRRQNGRGDEVSGYEDNDDDDDN 264
           AQ+ QRQS+AL K++ +GK TA  IL+RSRR+      RR+N R  E    E NDDDD N
Sbjct: 196 AQVSQRQSKALVKRKSVGKGTA--ILSRSRRSGGGSR-RRRNCRNIEQDTSEANDDDDQN 255

Query: 265 NEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANIDSGDGCAESDLDLS--RENRT 324
             GKDSSS DE      ++RKKR  T    QPSSS AN  + D CA +  + +  R++R 
Sbjct: 256 KRGKDSSS-DE---PCERQRKKRSAT----QPSSSNAN--NNDNCAGNGTEQTHQRDSRV 315

Query: 325 VSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLMKLAKYLRGLEENNNELDV 384
           +SP LV N+E++ WGRGG RSN+R G+    G  S R++RL +L +YL  LE N+ ELD+
Sbjct: 316 ISPVLVWNSELIAWGRGGTRSNTRQGN-NNQGAISKRNARLKRLVEYLGSLEGNSVELDI 375

Query: 385 HLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVSRKTPLQADDVEILSLKGRPRTSNE 444
           HL L+S+D E   +L +P+L  RP+L VK LREYV+R   L+A++VE+L           
Sbjct: 376 HLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELL----------- 435

Query: 445 QSTPSASISIDGMSLVFDPLKYE-LQSLEGEIFLAGLQSDCTYSRDLLILGYRRK 493
                  +S DG +++ +    E +QSL+ +  +A L+ DC  S   +I+ YRRK
Sbjct: 436 -------VSKDGDTVIGNKTSTEKMQSLQDDETVAKLKVDCISSNGYMIVVYRRK 457

BLAST of CSPI06G12750 vs. TAIR 10
Match: AT1G03770.3 (RING 1B )

HSP 1 Score: 325.9 bits (834), Expect = 5.8e-89
Identity = 231/481 (48.02%), Postives = 313/481 (65.07%), Query Frame = 0

Query: 25  QDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRVDEGEEEKQRQQLEEATGDRD--SE 84
           Q  + S  ER     +E+EE+ ++  +E+ + G   D+ + ++   + EEA G+ +   E
Sbjct: 16  QLGRNSEAERFNPEAVEKEEDPDKM-DEKDESGDEEDDVKRDQVEAEDEEALGEEEDSKE 75

Query: 85  GTQSSSSEE--KPEFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRL 144
            +QSSS+ E  + E++ V+L+ I KDVQC ICLGII+KTRTVMECLHRFCRECIDKSMRL
Sbjct: 76  RSQSSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRL 135

Query: 145 GNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEERNRNKQIQESI 204
           GNNECP CR HCASRRSLRDDPN+DALIAAL+ +IDK+EEEEL F +++  RNKQIQ SI
Sbjct: 136 GNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASI 195

Query: 205 AQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRRQNGRGDEVSGYEDNDDDDDN 264
           AQ+ QRQS+AL K++ +GK TA  IL+RSRR+      RR+N R  E    E NDDDD N
Sbjct: 196 AQVSQRQSKALVKRKSVGKGTA--ILSRSRRSGGGSR-RRRNCRNIEQDTSEANDDDDQN 255

Query: 265 NEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANIDSGDGCAESDLDLS--RENRT 324
             GKDSSS DE      ++RKKR  T    QPSSS AN  + D CA +  + +  R++R 
Sbjct: 256 KRGKDSSS-DE---PCERQRKKRSAT----QPSSSNAN--NNDNCAGNGTEQTHQRDSRV 315

Query: 325 VSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLMKLAKYLRGLEENNNELDV 384
           +SP LV N+E++ WGRGG RSN+R G+    G  S R++RL +L +YL  LE N+ ELD+
Sbjct: 316 ISPVLVWNSELIAWGRGGTRSNTRQGN-NNQGAISKRNARLKRLVEYLGSLEGNSVELDI 375

Query: 385 HLLLISVDKESTPSLQQPHLYCRPSLTVKHLRE------YVSRKTPLQADDVEILSLKGR 444
           HL L+S+D E   +L +P+L  RP+L VK LRE      YV+R   L+A++VE+L     
Sbjct: 376 HLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVARHLKLKAEEVELL----- 435

Query: 445 PRTSNEQSTPSASISIDGMSLVFDPLKYE-LQSLEGEIFLAGLQSDCTYSRDLLILGYRR 493
                        +S DG +++ +    E +QSL+ +  +A L+ DC  S   +I+ YRR
Sbjct: 436 -------------VSKDGDTVIGNKTSTEKMQSLQDDETVAKLKVDCISSNGYMIVVYRR 463

BLAST of CSPI06G12750 vs. TAIR 10
Match: AT1G03770.2 (RING 1B )

HSP 1 Score: 325.1 bits (832), Expect = 9.8e-89
Identity = 231/483 (47.83%), Postives = 313/483 (64.80%), Query Frame = 0

Query: 25  QDAKQSRTERETKTDIEEEEEEEEQREEQLKEGQRVDEGEEEKQRQQLEEATGDRD---- 84
           Q  + S  ER     +E+EE+ ++  +E+ + G   D+ + ++   + EEA G+ +    
Sbjct: 16  QLGRNSEAERFNPEAVEKEEDPDKM-DEKDESGDEEDDVKRDQVEAEDEEALGEEEVSDS 75

Query: 85  SEGTQSSSSEE--KPEFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSM 144
            E +QSSS+ E  + E++ V+L+ I KDVQC ICLGII+KTRTVMECLHRFCRECIDKSM
Sbjct: 76  KERSQSSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSM 135

Query: 145 RLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEERNRNKQIQE 204
           RLGNNECP CR HCASRRSLRDDPN+DALIAAL+ +IDK+EEEEL F +++  RNKQIQ 
Sbjct: 136 RLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQA 195

Query: 205 SIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNVHLRRQNGRGDEVSGYEDNDDDD 264
           SIAQ+ QRQS+AL K++ +GK TA  IL+RSRR+      RR+N R  E    E NDDDD
Sbjct: 196 SIAQVSQRQSKALVKRKSVGKGTA--ILSRSRRSGGGSR-RRRNCRNIEQDTSEANDDDD 255

Query: 265 DNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANIDSGDGCAESDLDLS--REN 324
            N  GKDSSS DE      ++RKKR  T    QPSSS AN  + D CA +  + +  R++
Sbjct: 256 QNKRGKDSSS-DE---PCERQRKKRSAT----QPSSSNAN--NNDNCAGNGTEQTHQRDS 315

Query: 325 RTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLMKLAKYLRGLEENNNEL 384
           R +SP LV N+E++ WGRGG RSN+R G+    G  S R++RL +L +YL  LE N+ EL
Sbjct: 316 RVISPVLVWNSELIAWGRGGTRSNTRQGN-NNQGAISKRNARLKRLVEYLGSLEGNSVEL 375

Query: 385 DVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLRE------YVSRKTPLQADDVEILSLK 444
           D+HL L+S+D E   +L +P+L  RP+L VK LRE      YV+R   L+A++VE+L   
Sbjct: 376 DIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVARHLKLKAEEVELL--- 435

Query: 445 GRPRTSNEQSTPSASISIDGMSLVFDPLKYE-LQSLEGEIFLAGLQSDCTYSRDLLILGY 493
                          +S DG +++ +    E +QSL+ +  +A L+ DC  S   +I+ Y
Sbjct: 436 ---------------VSKDGDTVIGNKTSTEKMQSLQDDETVAKLKVDCISSNGYMIVVY 465

BLAST of CSPI06G12750 vs. TAIR 10
Match: AT5G44280.2 (RING 1A )

HSP 1 Score: 301.2 bits (770), Expect = 1.5e-81
Identity = 216/500 (43.20%), Postives = 321/500 (64.20%), Query Frame = 0

Query: 10  DSVHGEDPRDQQFSHQDAKQSRTERETKTDIEEEE--EEEEQREEQLKEGQRVDEGEEEK 69
           D    E   D++  H +A++ + E     D EE++  ++ E+ EE+  E +  +E EEE+
Sbjct: 36  DETKEEKEGDEEVKHDEAEEDQ-EVVKPNDAEEDDDGDDAEEDEEEEVEAEEDEEAEEEE 95

Query: 70  QRQQLEEATGDRDSEGTQSSSSEEKPEFIFVELSGIRKDVQCPICLGIIKKTRTVMECLH 129
           + ++ EE   +   E + SS S ++ EF+ ++L  IRKDVQCPICL  +K  R  +   +
Sbjct: 96  EEEEEEEEEEEDSKERSPSSISGDQSEFMEIDLGEIRKDVQCPICLVGMKFLRLFIS-YY 155

Query: 130 RFCRECIDKSMR----LGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEEL 189
           +  ++C   S +      NNECPACR HCASRRSLRDDP +DALIAAL+ +ID YEEEEL
Sbjct: 156 KENKDCDGMSPQFCATFKNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEEL 215

Query: 190 TFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGV-----ILTRSRRNHRNVHL 249
            FHE+E  RNKQIQ SIAQI QRQSEAL K+R LGK+ A +     I + SRR   + ++
Sbjct: 216 AFHEDEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNM 275

Query: 250 RRQNGRGDEVSGYEDNDDDDDNNEGKD-SSSADERFTEVRQRRKKRHPTVRSSQ-PSSSI 309
            +QN        +ED+D+DD+NN G+D  SS+DER TEVRQ+++++  T RS+Q PSSS 
Sbjct: 276 EQQNAS----EAHEDDDNDDNNNRGRDKDSSSDERGTEVRQKKRRKRSTSRSTQHPSSSG 335

Query: 310 ANIDSGDGCAESDLDLSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHG-SAGGSGNKSSR 369
           AN ++G+ CA++D ++ R+ + +SPGLV N E+L WGRGG RSN+RHG +  G  +KS R
Sbjct: 336 ANKNNGN-CADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVR 395

Query: 370 SSRLMKLAKYLR-GLEENNNELDVHLLLISVDKESTPSLQQPHLYCRPSLTVKHLREYVS 429
           ++R+ KL +YLR  ++ ++ ELD+H+ L+S+D +  P L QP+L CRP+L VK LRE+V+
Sbjct: 396 NARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVA 455

Query: 430 RKTPLQADDVEILSLKGR-PRTSNEQSTPSASISIDGMSLVFDPLKYELQSLEGEIFLAG 489
            +  L+ ++VE+L  + R    +  ++ P+ + +    S      K E+ SLE    L+ 
Sbjct: 456 LQIHLKTEEVELLVTRRRVGEDAAIENLPAVTPASAAAS------KDEMLSLEDNETLSR 515

Query: 490 LQSDCTYSRDL-LILGYRRK 493
           L+ D   S +  LI+ YR+K
Sbjct: 516 LKIDFISSHEQHLIIAYRKK 522