CSPI06G08590 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G08590
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	cytochrome b5
Location	Chr6: 7220613 .. 7221850 (+)
RNA-Seq Expression	CSPI06G08590
Synteny	CSPI06G08590

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATAAATTGTTGTACTTTTGCTCACAATATTTACTTCTTTTCTTCTGCTTCTTCTTATCTTCTTCTATTTAAGGTCTAAAATATTGAAACTCTGTTGAAGGGAAGGATTAGAAAAAGAAAAAGAAAAGTATTTGATAGAAAATGGATTCAAATTCAAAAGTGTATTCCTTTGATGAAGTTGCAAAGCACAACCAGCAGAAGGATTGTTGGCTTATAATCTCTGGATATGTATGTTCTTTATTTCCCTTTTCTTCTTGTTGAATAACTCTCTCTACCTAATTTACAAATATATTCATTGCTTGATTAATTAGTATTTCTATTACATATCATCATAATAAGATTTGGATTAATGTTAATGCGCATTCTGTTGGGTCTAAGTTTTAGAAGAGTGTGAGTGTTGTTGTGTTTATAGTTAGTTCTTGTCTGAAGTGGTTTTGGAGGATTGTGAAGCTAAATGTGTATTGTTTTTAGGTGTACGACGTGACTTCATTTTTGCCGGACCATCCAGGCGGCGATGAGCTTCTGCTACTTGCAGTGGGTGAGATGGCTACACACCCATTTCTAATCATACAAATCCATATCTCTACTTAATTACTTTTTCTAATGTTTTATATATGCACATTAACATGGTTTACTTACTATACATTTTACTCTTTATACTTTTACAAATCTTAAAATTAGTCTTCTCTTGTTTCCTTCAAATTAAGATTCATGCAACTTTTCAAAGGATGACCCTGTAATCACCATCCAATTTCAACATTTTGTGTGCTACAAAAACGGATTCAGCTTACGTTTTCTTGTATTTACTTTAACATTTTCTAACGACTAGTTGAAGTATTTTGTGGATTAATTAACATGATAGATTTGAATATATGCAGAAAAAGATGCAACATTTGATTTTAAGAGTGTTGGACATAGCGAACTAGCTCATGAGAAGATGAAAATGTATCAAATAGGGAAGATCGACATGTCGACTCTCCCTGAAAAACAAAAGTACGTGGAAAGTGCACCATATCAACGAACTCCAAGTCTATTTTCCTTCTCACCCATCCATATTCTACTCCCTATTTTGCTCTTAGCCTTGGCTTTTGCATTTCCCTACTTCAAACTTAAGGCCTAAAATTCATATTATTTTACATTTCTATTTTACTTTCCCACAAGTATTATTATAGCCTATGGGCTATAAAATAACAATTTCTAGCAACTTCGTGATTGAATCGTTCAATGGATTTCTACC

mRNA sequence

GAATAAATTGTTGTACTTTTGCTCACAATATTTACTTCTTTTCTTCTGCTTCTTCTTATCTTCTTCTATTTAAGGTCTAAAATATTGAAACTCTGTTGAAGGGAAGGATTAGAAAAAGAAAAAGAAAAGTATTTGATAGAAAATGGATTCAAATTCAAAAGTGTATTCCTTTGATGAAGTTGCAAAGCACAACCAGCAGAAGGATTGTTGGCTTATAATCTCTGGATATGTGTACGACGTGACTTCATTTTTGCCGGACCATCCAGGCGGCGATGAGCTTCTGCTACTTGCAGTGGAAAAAGATGCAACATTTGATTTTAAGAGTGTTGGACATAGCGAACTAGCTCATGAGAAGATGAAAATGTATCAAATAGGGAAGATCGACATGTCGACTCTCCCTGAAAAACAAAAGTACGTGGAAAGTGCACCATATCAACGAACTCCAAGTCTATTTTCCTTCTCACCCATCCATATTCTACTCCCTATTTTGCTCTTAGCCTTGGCTTTTGCATTTCCCTACTTCAAACTTAAGGCCTAAAATTCATATTATTTTACATTTCTATTTTACTTTCCCACAAGTATTATTATAGCCTATGGGCTATAAAATAACAATTTCTAGCAACTTCGTGATTGAATCGTTCAATGGATTTCTACC

Coding sequence (CDS)

ATGGATTCAAATTCAAAAGTGTATTCCTTTGATGAAGTTGCAAAGCACAACCAGCAGAAGGATTGTTGGCTTATAATCTCTGGATATGTGTACGACGTGACTTCATTTTTGCCGGACCATCCAGGCGGCGATGAGCTTCTGCTACTTGCAGTGGAAAAAGATGCAACATTTGATTTTAAGAGTGTTGGACATAGCGAACTAGCTCATGAGAAGATGAAAATGTATCAAATAGGGAAGATCGACATGTCGACTCTCCCTGAAAAACAAAAGTACGTGGAAAGTGCACCATATCAACGAACTCCAAGTCTATTTTCCTTCTCACCCATCCATATTCTACTCCCTATTTTGCTCTTAGCCTTGGCTTTTGCATTTCCCTACTTCAAACTTAAGGCCTAA

Protein sequence

MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKSVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLALAFAFPYFKLKA*

Homology

[Close]

BLAST of CSPI06G08590 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 129.4 bits (324), Expect = 3.0e-29
Identity = 70/136 (51.47%), Postives = 90/136 (66.18%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+ KV SF+EV+KHN+ KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQ------RTPSLFSFSPIHILLP 120
            VGHS+ A + M  Y IG+ID S++P  + YV  AP Q      +TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

Query: 121 ILLLALAFAFPYFKLK 131
           IL+L LA    ++  K
Sbjct: 121 ILILGLALVVRHYTKK 133

[Close]

BLAST of CSPI06G08590 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 8.6e-29
Identity = 69/136 (50.74%), Postives = 88/136 (64.71%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S  KV  F+EV++HN+ KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQ------RTPSLFSFSPIHILLP 120
            VGHS+ A + M+ Y IG+ID ST+P  + YV  AP Q      +TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYV--APVQPAYNQDKTPE-FMIKILQFLVP 120

Query: 121 ILLLALAFAFPYFKLK 131
           IL+L LA     +  K
Sbjct: 121 ILILGLALVVRQYTKK 133

[Close]

BLAST of CSPI06G08590 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 8.1e-27
Identity = 61/130 (46.92%), Postives = 83/130 (63.85%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +KV++  EV++HN  KDCWL+ISG VYDVT FL DHPGGDE+LL A  KDAT DF+
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYV-ESAPY--QRTPSLFSFSPIHILLPILL 120
            VGHS  A   +  Y +G ID +T+P K KY   + P+  Q   S F    +  L+P+++
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LALAFAFPYF 128
           L +AF   ++
Sbjct: 121 LGVAFGIRFY 130

[Close]

BLAST of CSPI06G08590 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.8e-26
Identity = 58/124 (46.77%), Postives = 83/124 (66.94%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +K+++  EV++HNQ  DCW++I+G VY+VT FL DHPGGD++LL +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYV-ESAPY--QRTPSLFSFSPIHILLPILL 120
            VGHSE A E M+ Y +G+ID +T+P+K KY     P+  Q   S F    +  L+P+ +
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LALA 122
           L LA
Sbjct: 121 LGLA 124

[Close]

BLAST of CSPI06G08590 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 2.4e-26
Identity = 62/126 (49.21%), Postives = 81/126 (64.29%), Query Frame = 0


Query: 2   DSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKS 61
           + N KVY+ +EVAKHN + DCWLII G VY+V+ FL DHPGGD++LL +  KDAT DF+ 
Sbjct: 3   NDNKKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFED 62

Query: 62  VGHSELAHEKMKMYQIGKIDMSTLPEKQKYV-ESAPY---QRTPSLFSFSPIHILLPILL 121
           VGHS  A   M  Y +G ID ST+P + KYV    P+    +TP  F    +  L+P+ +
Sbjct: 63  VGHSTTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPE-FIIKILQFLVPLAI 122

Query: 122 LALAFA 124
           L LA A
Sbjct: 123 LGLAVA 127

[Close]

BLAST of CSPI06G08590 vs. ExPASy TrEMBL
Match: A0A0A0KA20 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G107940 PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 7.5e-68
Identity = 130/131 (99.24%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLAL 120
           SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIH+LLPILLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of CSPI06G08590 vs. ExPASy TrEMBL
Match: A0A1S3CAE5 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.1e-63
Identity = 123/131 (93.89%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHN QKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLAL 120
           SVGHSELA EKMKMY+IGKIDMSTLP+KQ YVES PYQ TPSLFSFSPIH+LLPILLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of CSPI06G08590 vs. ExPASy TrEMBL
Match: A0A6J1KHQ2 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.1e-53
Identity = 102/131 (77.86%), Postives = 116/131 (88.55%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSK+Y+F+EVAKHN +KDCWLIISGYVYDVTS+LPDHPGG ELL+L VEKDATFDFK
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLAL 120
           SVGHSE A E MK YQIGKID  TLP +QKYVE+ PY+ TPSL SFSP+H+L+P+LLL L
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of CSPI06G08590 vs. ExPASy TrEMBL
Match: A0A6J1HJ10 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 3.1e-53
Identity = 102/131 (77.86%), Postives = 118/131 (90.08%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVY+FDEVAKHN +KDCWLIISG+VYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLAL 120
           SVGHSE A E+MK YQIGKID  TLP +QK+VE+ P++ TPSL SFSP+H+L+P+LLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of CSPI06G08590 vs. ExPASy TrEMBL
Match: M5X125 (Cytochrome b5 heme-binding domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G194100 PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 3.5e-33
Identity = 78/135 (57.78%), Postives = 95/135 (70.37%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+S+VY FDEVAKHN +KDCW+IISG VYDVT FL DHPGGDE+LLLAVEKDAT DF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTP-----SLFSFSPIHILLPI 120
            VGHS+ A E+M+ + +GK+D ST+PE+  Y    P Q TP     S F    +  LLP+
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSY--KLPAQTTPPSGQSSGFVVKLLQFLLPL 120

Query: 121 LLLALAFAFPYFKLK 131
           L+L  AFA  Y+  K
Sbjct: 121 LILGAAFALQYYGKK 133

[Close]

BLAST of CSPI06G08590 vs. NCBI nr
Match: XP_004140502.1 (cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cucumis sativus])

HSP 1 Score: 266.2 bits (679), Expect = 1.6e-67
Identity = 130/131 (99.24%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLAL 120
           SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIH+LLPILLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of CSPI06G08590 vs. NCBI nr
Match: XP_008459741.1 (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 252.3 bits (643), Expect = 2.3e-63
Identity = 123/131 (93.89%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHN QKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLAL 120
           SVGHSELA EKMKMY+IGKIDMSTLP+KQ YVES PYQ TPSLFSFSPIH+LLPILLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of CSPI06G08590 vs. NCBI nr
Match: XP_038875836.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 233.8 bits (595), Expect = 8.5e-58
Identity = 111/131 (84.73%), Postives = 123/131 (93.89%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDS+SK+YSFDEV+KHN  +DCWLIISGYVYDVT+FLPDHPGG+ELLLLAV +DATFDFK
Sbjct: 3   MDSSSKLYSFDEVSKHNHHRDCWLIISGYVYDVTAFLPDHPGGEELLLLAVGRDATFDFK 62

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLAL 120
           SVGHSELA EKMK Y+IGKIDMSTLP KQ+YVE+ PYQ TPSLFSFSPIH+L+PILLLAL
Sbjct: 63  SVGHSELAQEKMKKYRIGKIDMSTLPRKQQYVETTPYQPTPSLFSFSPIHLLIPILLLAL 122

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 123 AFAFPYFKLKA 133

[Close]

BLAST of CSPI06G08590 vs. NCBI nr
Match: XP_023000205.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 219.2 bits (557), Expect = 2.2e-53
Identity = 102/131 (77.86%), Postives = 116/131 (88.55%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSK+Y+F+EVAKHN +KDCWLIISGYVYDVTS+LPDHPGG ELL+L VEKDATFDFK
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLAL 120
           SVGHSE A E MK YQIGKID  TLP +QKYVE+ PY+ TPSL SFSP+H+L+P+LLL L
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of CSPI06G08590 vs. NCBI nr
Match: XP_022964486.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 217.6 bits (553), Expect = 6.3e-53
Identity = 102/131 (77.86%), Postives = 118/131 (90.08%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVY+FDEVAKHN +KDCWLIISG+VYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHILLPILLLAL 120
           SVGHSE A E+MK YQIGKID  TLP +QK+VE+ P++ TPSL SFSP+H+L+P+LLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of CSPI06G08590 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 129.4 bits (324), Expect = 2.1e-30
Identity = 70/136 (51.47%), Postives = 90/136 (66.18%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+ KV SF+EV+KHN+ KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQ------RTPSLFSFSPIHILLP 120
            VGHS+ A + M  Y IG+ID S++P  + YV  AP Q      +TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

Query: 121 ILLLALAFAFPYFKLK 131
           IL+L LA    ++  K
Sbjct: 121 ILILGLALVVRHYTKK 133

[Close]

BLAST of CSPI06G08590 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 120.2 bits (300), Expect = 1.3e-27
Identity = 58/124 (46.77%), Postives = 83/124 (66.94%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +K+++  EV++HNQ  DCW++I+G VY+VT FL DHPGGD++LL +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYV-ESAPY--QRTPSLFSFSPIHILLPILL 120
            VGHSE A E M+ Y +G+ID +T+P+K KY     P+  Q   S F    +  L+P+ +
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LALA 122
           L LA
Sbjct: 121 LGLA 124

[Close]

BLAST of CSPI06G08590 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 119.4 bits (298), Expect = 2.2e-27
Identity = 61/137 (44.53%), Postives = 85/137 (62.04%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M  + KV++  EV++H+  KDCW++I G VYDVT FL DHPGGDE++L +  KDAT DF+
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYV-----ESAPYQRTPSLFSFSPIHILLPI 120
            VGHS  A   +  Y +G ID +T+P K K+V     ++   Q   S F    +  L+P+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 LLLALAFAFPYF-KLKA 132
           L+L LAF   Y+ K KA
Sbjct: 121 LILGLAFGIRYYTKTKA 137

[Close]

BLAST of CSPI06G08590 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 110.9 bits (276), Expect = 7.8e-25
Identity = 52/126 (41.27%), Postives = 82/126 (65.08%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M + +K+YS +E A HN+Q DCW++I G VYDV+S++ +HPGGD++LL    KDAT DF+
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQK---YVESAPYQRTPSLFSFSPIHILLPILL 120
             GHS+ A E M+ Y IG++D S+LPE  +   Y +  P      LF  +  + ++P+ +
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 LALAFA 124
           + ++ A
Sbjct: 121 ITISVA 126

[Close]

BLAST of CSPI06G08590 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 109.8 bits (273), Expect = 1.7e-24
Identity = 54/131 (41.22%), Postives = 81/131 (61.83%), Query Frame = 0


Query: 5   SKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKSVGH 64
           + + SF +VAKH  + DCW++I G VYD+++F+ +HPGGD +LL    KDA+ DF+ V H
Sbjct: 2   ANLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNH 61

Query: 65  SELAHEKMKMYQIGKIDMSTLPEKQKYV-----ESAPYQRTPSLFSFSPIHILLPILLLA 124
           S+ A E MK Y IG +D ST+P  Q+Y+     ES   + T        +  L+P+L+L 
Sbjct: 62  SKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILG 121

Query: 125 LAFAFPYFKLK 131
           +AFA  ++  K
Sbjct: 122 VAFALRFYNNK 132

The following BLAST results are available for this feature:

BLAST of CSPI06G08590 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42342	3.0e-29	51.47	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P40934	8.6e-29	50.74	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
P49098	8.1e-27	46.92	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
O48845	1.8e-26	46.77	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
P49100	2.4e-26	49.21	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]

BLAST of CSPI06G08590 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KA20	7.5e-68	99.24	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A1S3CAE5	1.1e-63	93.89	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1	[more]
A0A6J1KHQ2	1.1e-53	77.86	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1	[more]
A0A6J1HJ10	3.1e-53	77.86	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1	[more]
M5X125	3.5e-33	57.78	Cytochrome b5 heme-binding domain-containing protein OS=Prunus persica OX=3760 G...	[more]

BLAST of CSPI06G08590 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140502.1	1.6e-67	99.24	cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cuc...	[more]
XP_008459741.1	2.3e-63	93.89	PREDICTED: cytochrome b5 [Cucumis melo]	[more]
XP_038875836.1	8.5e-58	84.73	cytochrome b5-like [Benincasa hispida]	[more]
XP_023000205.1	2.2e-53	77.86	cytochrome b5-like [Cucurbita maxima]	[more]
XP_022964486.1	6.3e-53	77.86	cytochrome b5-like [Cucurbita moschata]	[more]

BLAST of CSPI06G08590 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53560.1	2.1e-30	51.47	cytochrome B5 isoform E	[more]
AT2G32720.1	1.3e-27	46.77	cytochrome B5 isoform B	[more]
AT5G48810.1	2.2e-27	44.53	cytochrome B5 isoform D	[more]
AT1G26340.1	7.8e-25	41.27	cytochrome B5 isoform A	[more]
AT2G46650.1	1.7e-24	41.22	cytochrome B5 isoform C	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 30..40 score: 64.74 coord: 55..62 score: 35.8 coord: 40..54 score: 44.55 coord: 68..80 score: 32.98
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 9.1E-26 score: 101.6
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 e-value: 7.5E-22 score: 77.3
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 26.633057
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..85 e-value: 4.4E-31 score: 108.7
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..88
None	No IPR available	PANTHER	PTHR19359:SF81	SUBFAMILY NOT NAMED	coord: 1..129
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..129
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G08590.1	CSPI06G08590.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding