CSPI06G05890 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G05890
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	vestitone reductase-like
Location	Chr6: 5240150 .. 5243653 (-)
RNA-Seq Expression	CSPI06G05890
Synteny	CSPI06G05890

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAGTGTGGCGAAGGGGAAAGTTTGTGTAACTGGAGGTACTGGATTCGTAGCTTCATGGCTAATCAAGAGGCTTCTTGAAAATGGCTACTCTGTCACTACCACTGTTAGAGCCGACCCAGGTATTTTCTCATTTCTATTTTTTGGTTTCTGTTTTCTGTTTTCCTTTCTTAGATTTTCATGGTTGAGACTTTCTGATGCTGGTTATTTGCTATTAGTGGTAATTTATTCAAACTTGCTGCTGGGTTATCCTATCTTATTATTGGATTGTTAAGGATAAAATTATTTGGTAAGAATGAGTGGCTTTGAGTCTTAGAAATGGGCTTTGAATTGGGATTTCTTTTCCCTGAGCTCTACTTGAGTCATTTGAATATTATTCGAGTCTTTTTGTCCTGCTTCTTACTGCTATGGATTCTTTACTTCATTTCACTTCTTTGACTTCTTCTGAGGTTTGCATGTTTGGTGTTTGATGAAATTACTGTGAGAGTCGATCATCTCACTGCTATTTATGCACAGTGGCTTCGACTGATTACTCTTTTGAAACAGTTCAAATATTAAACCAGTAGGTTATAGAATATTTAATTTTCATACATCACATGTTCTTAGCACCATCCCATCATTACTTGTGCGTTTAGGAAAGTCAAGTGGTAGAGCAGTTTTCATGGCCCCTCTTTCAACTCAGTTTGTGGGTTGAAGTCTTGGTTTTTACACTACGTGTGGGTTTAGTTATATTGACCAGAACATGATGTCATGGGAACACAGAACTATTTATACTAAACAGTCACCAAGTTCAAGTGGATAGGTTGGATATTATATTATTGCCGACCCTCACGAGTCACCTACTAGTAAATAAAGGATTTAATGTCTATGGTGCGACTTACTTAAGATTTAATAATTTGTGGAAACTTAATGTTTAACTCGGGTAGGATTTGACAAATTATCTTGTAATATTAGCTGAGATGTAGGTAAGTTGAACAGAACATTCATAAACATTCTAATAACAATAATAAGAAAGTATATAATATCCTTGCCTTAAAAGACTAATTATTATAGTTTTGTTTGTAGCATCTGCTTAATTTTAAGAGATTGCCCTAATTTATTTGAAGTTTTTAGAATTCGTTAAGGATTTTAAGAGGTAGATCAGACTACAACTGAAGTTATCAGTGAACAAGTATGAATTTATAAACAGGCCACCGAGACAAAATCATTATCAAATAGGTTATAAGAGATATATTTCTATCACTGAATAGATCAATTGTCGAACTTGGTTTTCCTCATCTAAATTTGCTTTCATTTTAATTTACACAGAGAAGAGAAAAGATTACAGCTTCTTGACAAATCTACCCGGAGCATCAGAAAAGCTGCAAATATACCAGGCTGATCTTCACGACCCGAACAGCTTTGCTCCTGCCATTGCAGGTTGCATTGGAGTCTTTCATCTTGCTACCCCAATTGACGTGGATGACAAAGAACCTTTGGAATCAGTGACGAGACGAACGATTGAAGGAACATTGGGCATCCTTAAACTTTCTGTAGATTCGAAAACAGTAAGGCGAGTTGTATACACTTCCAGTGCAGCCACCATGCAGTTTAATCACCACAAAGTAGACTTCTTGGATGAAAGCTGTTGGAGTGACATTGATTACATCAACAGTATTGCACCGTTGGGAAGATCATACCCGATTTCTAAGACTTTAACCGAGAAAGCAGTTCTTGAGTTCTCTCAGCAATATGGATTAGAAGTTGTTACTGTCCTTCCAACGTATGTCGTTGGTCCCTTCATTTGTCCTAAAATTCCCGGCTCTGTTCATGTGATACTGTCTCTGATATTAGGTATTTGTTGTTTTCACTTTTAAAGTATCTTATGCTCTTACTTATAGATTTGGAAATCTGTTAGTACGGACCCTCAAGAACAACAAAAAACATAAAGAATACTAAAAGTAGAAATATATATTGCAATATCAATGGAGAAACTACAGTATACGCTAGCCTTTTGAGAGAGCTTCTCTCTCCCAACTTCCCTCACTGGAAATTACTACTAAAATTTTCAGACCTTGCTTCCAACTCACCTCATCTATTTATATCCTAAAAGTCCTAACAAACTTATTAGTTAATTACTAATATGCTCTTTTCCTTTTAATAGCCATACTAATAATTCTAATATTTTTCGAGCTAGTCTTACAAAATCTACACAGTTTTGTAGGCAGAGTATGGATTGATCTTAGTTCCAAAATGATCTTAACATGTGACTTTTGCAGGTAATGAAACAGAGTACGGATTGATCTTAAAATCAAACATGGTGCATGTGGATGATGTGGCAAGAGCACATATTTATCTATTTGAAAATCCAAATGCAAGTGGGCGATATGTTTGTTCTTCTCACATAATCACATTGGAAGAATTGGCTAACTTCTTCTCTGCCAAGTACCCAGAGTTTCAGATACCATCTCCCGAGTGAGTTCCACTACGTATTACTTTCCCTTGTTACAATTTTTTTGAAAATATAGTTTTCGTTTGTATATATATCTATATCTATATATATATTAACTTTTAACTAAAATTGAGTTTATAAACACTATTTCTACCCACTGATTTATTTGTTTTTTTTGTCTACCTTTTACAAGTTTTCAGATTTCGAACCAATTTTTGAAAACTAACGGAGGAGTTTGCAAAACTAGCAAAAAAAATTACGATAATAGAGTCTATGTCACCTACATTTTCTAAATTGTAAAAATGACAAAATCTGATTACCATCAATTACTAGTGATATTTGTCAAATTTGCAATATGAAAAAAAACGTAGTTTTTAAAACTTGTTTTTGTTTTTGGAGTTTGACTAAAAATTCAAACTTCTATCTAAGAAGATGAAAACCATGGTAAAGAGATTGTGATGAACATGTAAAATTTTCAAAAACAAATAAATAGAATAGTTATCAACTTGTGGATTTTGATTATGGTGTCTATTCATTCATTTTTTTAAATTGTGACTCTATGCACCCATCCATTCTCGTAGGTAACTAAACAATTATATGGTATGCTTATTGAATAAATATTGATTGATTGCATGACTTGATATACAAATTATATGAGAGTTGATACCTGGTAAAAATAAAGTTGCTTGTACTGTTACCCCCTTATTTGAAACATCGTATCAAATCTCATTTATAAAATTATTTGTCTACATGTTCACTATTTTTTTTGAATCGTTTATTTGTCAAATGCCAAGTTTTTCTTCTATTAAACACATCGGATCTTCATCATCTAGTTCATAACACGGAATATGAAGTTTCAAAACTCAAAGTGAGACGTAGTTACTTATGTTGAGTTTAGAGATTAAATAAATACAACTGGCTTGTTGCAGGTCTTTGAAAGATGTCAAAGGCTACATATTCACTGATGTCTCATCAAAGAAGCTTTTGGATGCTGGTTTTCAATACAAGTATGGTGTTGAAGAAATGCTTGATGGAGCAATCCAAAGCTGCAAAGAGAAGGGTTATCTCTAG

mRNA sequence

ATGGAAAGTGTGGCGAAGGGGAAAGTTTGTGTAACTGGAGGTACTGGATTCGTAGCTTCATGGCTAATCAAGAGGCTTCTTGAAAATGGCTACTCTGTCACTACCACTGTTAGAGCCGACCCAGAGAAGAGAAAAGATTACAGCTTCTTGACAAATCTACCCGGAGCATCAGAAAAGCTGCAAATATACCAGGCTGATCTTCACGACCCGAACAGCTTTGCTCCTGCCATTGCAGGTTGCATTGGAGTCTTTCATCTTGCTACCCCAATTGACGTGGATGACAAAGAACCTTTGGAATCAGTGACGAGACGAACGATTGAAGGAACATTGGGCATCCTTAAACTTTCTGTAGATTCGAAAACAGTAAGGCGAGTTGTATACACTTCCAGTGCAGCCACCATGCAGTTTAATCACCACAAAGTAGACTTCTTGGATGAAAGCTGTTGGAGTGACATTGATTACATCAACAGTATTGCACCGTTGGGAAGATCATACCCGATTTCTAAGACTTTAACCGAGAAAGCAGTTCTTGAGTTCTCTCAGCAATATGGATTAGAAGTTGTTACTGTCCTTCCAACGTATGTCGTTGGTCCCTTCATTTGTCCTAAAATTCCCGGCTCTGTTCATGTGATACTGTCTCTGATATTAGGTAATGAAACAGAGTACGGATTGATCTTAAAATCAAACATGGTGCATGTGGATGATGTGGCAAGAGCACATATTTATCTATTTGAAAATCCAAATGCAAGTGGGCGATATGTTTGTTCTTCTCACATAATCACATTGGAAGAATTGGCTAACTTCTTCTCTGCCAAGTACCCAGAGTTTCAGATACCATCTCCCGAGTCTTTGAAAGATGTCAAAGGCTACATATTCACTGATGTCTCATCAAAGAAGCTTTTGGATGCTGGTTTTCAATACAAGTATGGTGTTGAAGAAATGCTTGATGGAGCAATCCAAAGCTGCAAAGAGAAGGGTTATCTCTAG

Coding sequence (CDS)

ATGGAAAGTGTGGCGAAGGGGAAAGTTTGTGTAACTGGAGGTACTGGATTCGTAGCTTCATGGCTAATCAAGAGGCTTCTTGAAAATGGCTACTCTGTCACTACCACTGTTAGAGCCGACCCAGAGAAGAGAAAAGATTACAGCTTCTTGACAAATCTACCCGGAGCATCAGAAAAGCTGCAAATATACCAGGCTGATCTTCACGACCCGAACAGCTTTGCTCCTGCCATTGCAGGTTGCATTGGAGTCTTTCATCTTGCTACCCCAATTGACGTGGATGACAAAGAACCTTTGGAATCAGTGACGAGACGAACGATTGAAGGAACATTGGGCATCCTTAAACTTTCTGTAGATTCGAAAACAGTAAGGCGAGTTGTATACACTTCCAGTGCAGCCACCATGCAGTTTAATCACCACAAAGTAGACTTCTTGGATGAAAGCTGTTGGAGTGACATTGATTACATCAACAGTATTGCACCGTTGGGAAGATCATACCCGATTTCTAAGACTTTAACCGAGAAAGCAGTTCTTGAGTTCTCTCAGCAATATGGATTAGAAGTTGTTACTGTCCTTCCAACGTATGTCGTTGGTCCCTTCATTTGTCCTAAAATTCCCGGCTCTGTTCATGTGATACTGTCTCTGATATTAGGTAATGAAACAGAGTACGGATTGATCTTAAAATCAAACATGGTGCATGTGGATGATGTGGCAAGAGCACATATTTATCTATTTGAAAATCCAAATGCAAGTGGGCGATATGTTTGTTCTTCTCACATAATCACATTGGAAGAATTGGCTAACTTCTTCTCTGCCAAGTACCCAGAGTTTCAGATACCATCTCCCGAGTCTTTGAAAGATGTCAAAGGCTACATATTCACTGATGTCTCATCAAAGAAGCTTTTGGATGCTGGTTTTCAATACAAGTATGGTGTTGAAGAAATGCTTGATGGAGCAATCCAAAGCTGCAAAGAGAAGGGTTATCTCTAG

Protein sequence

MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKLQIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSKTVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFSQQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAHIYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKLLDAGFQYKYGVEEMLDGAIQSCKEKGYL*

Homology

[Close]

BLAST of CSPI06G05890 vs. ExPASy Swiss-Prot
Match: Q40316 (Vestitone reductase OS=Medicago sativa OX=3879 PE=1 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 5.9e-111
Identity = 188/323 (58.20%), Postives = 246/323 (76.16%), Query Frame = 0


Query: 6   KGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKLQIYQA 65
           KG+VCVTGGTGF+ SW+IK LLENGYSV TT+RADPE+++D SFLTNLPGASEKL  + A
Sbjct: 5   KGRVCVTGGTGFLGSWIIKSLLENGYSVNTTIRADPERKRDVSFLTNLPGASEKLHFFNA 64

Query: 66  DLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSKTVRRV 125
           DL +P+SFA AI GC+G+FH A+PID    EP E VT+RT++G LGILK  V+SKTV+R 
Sbjct: 65  DLSNPDSFAAAIEGCVGIFHTASPIDFAVSEPEEIVTKRTVDGALGILKACVNSKTVKRF 124

Query: 126 VYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFSQQYGL 185
           +YTSS + + FN    D LDES WSD+D + S+ P G +Y +SKTL EKAVLEF +Q G+
Sbjct: 125 IYTSSGSAVSFNGKDKDVLDESDWSDVDLLRSVKPFGWNYAVSKTLAEKAVLEFGEQNGI 184

Query: 186 EVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAHIYLFE 245
           +VVT++  ++VG F+CPK+P S+   L L+LG + + G + + +MVHVDDVARAHIYL E
Sbjct: 185 DVVTLILPFIVGRFVCPKLPDSIEKALVLVLGKKEQIG-VTRFHMVHVDDVARAHIYLLE 244

Query: 246 NPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKLLDAGF 305
           N    GRY CS  I+ +EE++   SAKYPE+QI + + LK++KG    D+++KKL+DAGF
Sbjct: 245 NSVPGGRYNCSPFIVPIEEMSQLLSAKYPEYQILTVDELKEIKGARLPDLNTKKLVDAGF 304

Query: 306 QYKYGVEEMLDGAIQSCKEKGYL 329
            +KY +E+M D AIQ CKEKGYL
Sbjct: 305 DFKYTIEDMFDDAIQCCKEKGYL 326

[Close]

BLAST of CSPI06G05890 vs. ExPASy Swiss-Prot
Match: O22133 (Protein BRI1-5 ENHANCED 1 OS=Arabidopsis thaliana OX=3702 GN=BEN1 PE=2 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.1e-83
Identity = 166/328 (50.61%), Postives = 221/328 (67.38%), Query Frame = 0


Query: 7   GKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPE-KRKDYSFLTNLPGASEKLQIYQA 66
           G VCVTGG+GFVASWLI RLL+ GYSV  TVR + E  +KD S+LT LP ASE+LQI+ A
Sbjct: 38  GLVCVTGGSGFVASWLIMRLLQRGYSVRATVRTNSEGNKKDISYLTELPFASERLQIFTA 97

Query: 67  DLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSKTVRRV 126
           DL++P SF PAI GC  VFH+A P+D +  E  E+VT+RT++G +GILK  +D+KTV+R 
Sbjct: 98  DLNEPESFKPAIEGCKAVFHVAHPMDPNSNETEETVTKRTVQGLMGILKSCLDAKTVKRF 157

Query: 127 VYTSSAATMQF---NHHKVDFLDESCWSDIDYINSIAP--LGRSYPISKTLTEKAVLEFS 186
            YTSSA T+ +   N      +DES WSD++   +     +  SY +SK   E A LEF 
Sbjct: 158 FYTSSAVTVFYSGGNGGGGGEVDESVWSDVEVFRNQKEKRVSSSYVVSKMAAETAALEFG 217

Query: 187 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 246
            + GLEVVT++   VVGPFI   +P SV + L+++ GN  E  L    NMVH+DDVARA 
Sbjct: 218 GKNGLEVVTLVIPLVVGPFISSSLPSSVFISLAMLFGNYKEKYLFDTYNMVHIDDVARAM 277

Query: 247 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 306
           I+L E P A GRY+CSS  + ++E+  F S K+P+FQ+PS + L   K      +SSKKL
Sbjct: 278 IFLLEKPVAKGRYICSSVEMKIDEVFEFLSTKFPQFQLPSID-LNKYKVEKRMGLSSKKL 337

Query: 307 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
             AGF++KYG EE+  GAI+SC+ +G+L
Sbjct: 338 KSAGFEFKYGAEEIFSGAIRSCQARGFL 364

[Close]

BLAST of CSPI06G05890 vs. ExPASy Swiss-Prot
Match: Q5XLY0 (Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.9e-80
Identity = 149/329 (45.29%), Postives = 219/329 (66.57%), Query Frame = 0


Query: 9   VCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKLQIYQADLH 68
           VCVTG  GF+ASWL+KRLLE GY V  TVR DPE +   S L NLPGA+++L++++A+L 
Sbjct: 15  VCVTGAAGFMASWLVKRLLEKGYIVHATVR-DPENKAKVSHLLNLPGATDRLKLFRAELC 74

Query: 69  DPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSKTVRRVVYT 128
           +  SF  A+AGC GVFH+ATP +   K+P   + +  IEGTL +LK      +++RVV T
Sbjct: 75  EDGSFDAAVAGCNGVFHVATPTEFMPKDPENDLIKPAIEGTLNVLKSCTKVDSIKRVVVT 134

Query: 129 SSAATMQFNH--HKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFSQQYGLE 188
           SSAAT+  N+   +  ++DESCW+D++++ S  P G +YP+SKTL E+A L++++++ L+
Sbjct: 135 SSAATVSINNSSEQNQYIDESCWTDVNFLTSQKPPGWAYPVSKTLAEQAALKYAEEHSLD 194

Query: 189 VVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLIL-------KSNMVHVDDVARA 248
           VVTV+P  VVGP + P +P SV + LSLI G+E + G +          ++VH+DDV  A
Sbjct: 195 VVTVIPVLVVGPAVTPTVPSSVELALSLITGDEFKMGALKGMQFVSGSISLVHIDDVCSA 254

Query: 249 HIYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKK 308
            I+L E P+A GRY+C      + +LA F S +YP++++P+     DV       +SS+K
Sbjct: 255 QIFLMEKPSAQGRYICFPVNTGIPQLAEFLSKRYPQYKVPT--KFDDVPATPKLTISSQK 314

Query: 309 LLDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           LLD GF +KYG+E++ D AI+  K KG L
Sbjct: 315 LLDCGFSFKYGIEDIYDQAIEYMKTKGLL 340

[Close]

BLAST of CSPI06G05890 vs. ExPASy Swiss-Prot
Match: P51110 (Dihydroflavonol 4-reductase OS=Vitis vinifera OX=29760 GN=DFR PE=1 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.9e-80
Identity = 151/325 (46.46%), Postives = 206/325 (63.38%), Query Frame = 0


Query: 9   VCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKLQIYQADLH 68
           VCVTG +GF+ SWL+ RLLE   +V  TVR DP   K    L +LP A   L +++ADL 
Sbjct: 8   VCVTGASGFIGSWLVMRLLERRLTVRATVR-DPTNVKKVKHLLDLPKAETHLTLWKADLA 67

Query: 69  DPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSKTVRRVVYT 128
           D  SF  AI GC GVFH+ATP+D + K+P   V + TIEG LGI+K    +KTVRR+V+T
Sbjct: 68  DEGSFDEAIKGCTGVFHVATPMDFESKDPENEVIKPTIEGMLGIMKSCAAAKTVRRLVFT 127

Query: 129 SSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFSQQYGLEVV 188
           SSA T+    H++   DESCWSD+++  +       Y +SKTL E+A  +++++  ++ +
Sbjct: 128 SSAGTVNIQEHQLPVYDESCWSDMEFCRAKKMTAWMYFVSKTLAEQAAWKYAKENNIDFI 187

Query: 189 TVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAHIYLFENPN 248
           T++PT VVGPFI   +P S+   LS I GNE  Y +I +   VH+DD+  AHIYLFENP 
Sbjct: 188 TIIPTLVVGPFIMSSMPPSLITALSPITGNEAHYSIIRQGQFVHLDDLCNAHIYLFENPK 247

Query: 249 ASGRYVCSSHIITLEELANFFSAKYPEFQIPS-----PESLKDVKGYIFTDVSSKKLLDA 308
           A GRY+CSSH   + +LA     KYPE+ IP+      E+LK V        SSKKL D 
Sbjct: 248 AEGRYICSSHDCIILDLAKMLREKYPEYNIPTEFKGVDENLKSV------CFSSKKLTDL 307

Query: 309 GFQYKYGVEEMLDGAIQSCKEKGYL 329
           GF++KY +E+M  GA+ +C+ KG L
Sbjct: 308 GFEFKYSLEDMFTGAVDTCRAKGLL 325

[Close]

BLAST of CSPI06G05890 vs. ExPASy Swiss-Prot
Match: Q9XES5 (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestica OX=3750 GN=DFR PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 9.2e-80
Identity = 145/321 (45.17%), Postives = 209/321 (65.11%), Query Frame = 0


Query: 9   VCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKLQIYQADLH 68
           VCVTG +GF+ SWL+ RLLE+GY+V  TVR DP  +K    L +LP A   L +++ADL 
Sbjct: 8   VCVTGASGFIGSWLVMRLLEHGYTVRATVR-DPTNQKKVKHLLDLPKAETHLTLWKADLA 67

Query: 69  DPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSKTVRRVVYT 128
           D  SF  AI GC GVFH+ATP+D + K+P   V + TI G L ILK    +KTVR++V+T
Sbjct: 68  DEGSFDEAIQGCSGVFHVATPMDFESKDPENEVIKPTINGLLDILKACQKAKTVRKLVFT 127

Query: 129 SSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFSQQYGLEVV 188
           SSA T+    H+    DES WSD+++  S+   G  Y +SKTL E+A  +++++  ++ +
Sbjct: 128 SSAGTVNVEEHQKPVYDESNWSDVEFCRSVKMTGWMYFVSKTLAEQAAWKYAKENNIDFI 187

Query: 189 TVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAHIYLFENPN 248
           T++PT V+GPF+ P +P S+   LS IL NE+ YG+I +   VH+DD+  +HIYL+E+P 
Sbjct: 188 TIIPTLVIGPFLMPSMPPSLITGLSPILRNESHYGIIKQGQYVHLDDLCLSHIYLYEHPK 247

Query: 249 ASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIF-TDVSSKKLLDAGFQY 308
           A GRY+CSSH  T+ EL      KYPE+ IP+    K +   +     SSKKL + GF++
Sbjct: 248 AEGRYICSSHDATIHELVKMLREKYPEYNIPT--KFKGIDDNLEPVHFSSKKLREIGFEF 307

Query: 309 KYGVEEMLDGAIQSCKEKGYL 329
           KY +E+M  GA+ +C+ KG +
Sbjct: 308 KYSLEDMFVGAVDACRAKGLI 325

[Close]

BLAST of CSPI06G05890 vs. ExPASy TrEMBL
Match: A0A0A0K9S9 (Cinnamoyl-CoA reductase 16 OS=Cucumis sativus OX=3659 GN=CCR16 PE=2 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 5.3e-187
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60
           MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL
Sbjct: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60

Query: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK 120
           QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK
Sbjct: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK 120

Query: 121 TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS 180
           TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS
Sbjct: 121 TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 240
           QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 240

Query: 241 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300
           IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL
Sbjct: 241 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300

Query: 301 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           LDAGFQYKYGVEEMLDGAIQSCKEKGYL
Sbjct: 301 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 328

[Close]

BLAST of CSPI06G05890 vs. ExPASy TrEMBL
Match: A0A5D3DLI4 (Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00450 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.0e-182
Identity = 317/328 (96.65%), Postives = 327/328 (99.70%), Query Frame = 0


Query: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60
           MES AKGKVCVTGGTGFVASWLIK+LLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL
Sbjct: 1   MESAAKGKVCVTGGTGFVASWLIKKLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60

Query: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK 120
           QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEP+ES+TRRTIEGTLGILKLSVDSK
Sbjct: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPVESLTRRTIEGTLGILKLSVDSK 120

Query: 121 TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS 180
           TVRRVVYTSSAATMQFNHHKVDFLDE+CWSDIDYIN+IAPLGRSYPISKTLTEKAVLEFS
Sbjct: 121 TVRRVVYTSSAATMQFNHHKVDFLDENCWSDIDYINNIAPLGRSYPISKTLTEKAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 240
           QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNE+EYGLILKSNMVHVDDVARAH
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNESEYGLILKSNMVHVDDVARAH 240

Query: 241 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300
           IYLFE+P+ASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL
Sbjct: 241 IYLFEHPSASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300

Query: 301 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           LDAGFQYKYG+EEMLDGAI+SCKEKGYL
Sbjct: 301 LDAGFQYKYGIEEMLDGAIESCKEKGYL 328

[Close]

BLAST of CSPI06G05890 vs. ExPASy TrEMBL
Match: A0A1S3CBT5 (vestitone reductase-like OS=Cucumis melo OX=3656 GN=LOC103499024 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.0e-182
Identity = 317/328 (96.65%), Postives = 327/328 (99.70%), Query Frame = 0


Query: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60
           MES AKGKVCVTGGTGFVASWLIK+LLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL
Sbjct: 1   MESAAKGKVCVTGGTGFVASWLIKKLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60

Query: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK 120
           QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEP+ES+TRRTIEGTLGILKLSVDSK
Sbjct: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPVESLTRRTIEGTLGILKLSVDSK 120

Query: 121 TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS 180
           TVRRVVYTSSAATMQFNHHKVDFLDE+CWSDIDYIN+IAPLGRSYPISKTLTEKAVLEFS
Sbjct: 121 TVRRVVYTSSAATMQFNHHKVDFLDENCWSDIDYINNIAPLGRSYPISKTLTEKAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 240
           QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNE+EYGLILKSNMVHVDDVARAH
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNESEYGLILKSNMVHVDDVARAH 240

Query: 241 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300
           IYLFE+P+ASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL
Sbjct: 241 IYLFEHPSASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300

Query: 301 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           LDAGFQYKYG+EEMLDGAI+SCKEKGYL
Sbjct: 301 LDAGFQYKYGIEEMLDGAIESCKEKGYL 328

[Close]

BLAST of CSPI06G05890 vs. ExPASy TrEMBL
Match: A0A6J1KZ90 (vestitone reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111498944 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.1e-166
Identity = 286/328 (87.20%), Postives = 309/328 (94.21%), Query Frame = 0


Query: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60
           ME+ AKGKVCVTGGTGF+ASWLIK LLENGYSVTTTVRADPE R+DY FLTNLPGASEKL
Sbjct: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60

Query: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK 120
           QIY ADL DPNSFAPAIAGCIGVFHLATPID+ DKE +E +TRRTIEGTLGILK+ +DSK
Sbjct: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120

Query: 121 TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS 180
           TVRRVVYTS+AATMQFNHH VDFLDESCWSDIDYINSIAPLGRSYPISKTLTE+AVLEFS
Sbjct: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 240
           QQYGLEVVTVLPTYVVGPFICP++P S+ V  SL+ GN+ EYGLILKSNMVHVDDVAR+H
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240

Query: 241 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300
           I+LFE+PNA+GRYVCSSH+IT+EELAN FSAKYPEFQIPSPESLKDVKGYIF+DVSSKKL
Sbjct: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300

Query: 301 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           LDAGFQYKYG+EEM DGAIQSCKEKGYL
Sbjct: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYL 328

[Close]

BLAST of CSPI06G05890 vs. ExPASy TrEMBL
Match: A0A6J1H5D7 (vestitone reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111460207 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 5.3e-163
Identity = 282/328 (85.98%), Postives = 304/328 (92.68%), Query Frame = 0


Query: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60
           ME+ AKGKVCVTGGTGF+ASWLIK LLE GYSVTTTVRADPE RKDY FLT+LPGASEKL
Sbjct: 1   MENGAKGKVCVTGGTGFIASWLIKSLLEKGYSVTTTVRADPEMRKDYGFLTSLPGASEKL 60

Query: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK 120
           QIY ADL +PNSFAPAIAGCIGVFHLATPID  DKE +E +TRRTIEGTLGILK+ +DSK
Sbjct: 61  QIYHADLDEPNSFAPAIAGCIGVFHLATPIDASDKEGVEVLTRRTIEGTLGILKVCLDSK 120

Query: 121 TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS 180
           TVRRVVYTS+AATMQFNHH VDFLDESCWSDIDYINSIAP GRSYPISKTLTE+AVLEFS
Sbjct: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPFGRSYPISKTLTERAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 240
           QQYGLEVVTVLPTYVVGPFICP++P S+ V  SL+ GN+ +YGLILKSNMVHVDDVARAH
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKADYGLILKSNMVHVDDVARAH 240

Query: 241 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300
           I+LFE+PNA+GRYVCSSHIITLEEL N FSAKYPEFQIPS ESLKDVKGY+F+DVSSKKL
Sbjct: 241 IFLFEHPNANGRYVCSSHIITLEELVNLFSAKYPEFQIPSSESLKDVKGYLFSDVSSKKL 300

Query: 301 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           LDAGFQYKYG+EEM DGAIQSCKEKGYL
Sbjct: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYL 328

[Close]

BLAST of CSPI06G05890 vs. NCBI nr
Match: XP_004145001.1 (vestitone reductase [Cucumis sativus] >QHJ91613.1 cinnamoyl-CoA reductase 16 [Cucumis sativus])

HSP 1 Score: 663.3 bits (1710), Expect = 1.1e-186
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60
           MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL
Sbjct: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60

Query: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK 120
           QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK
Sbjct: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK 120

Query: 121 TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS 180
           TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS
Sbjct: 121 TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 240
           QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 240

Query: 241 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300
           IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL
Sbjct: 241 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300

Query: 301 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           LDAGFQYKYGVEEMLDGAIQSCKEKGYL
Sbjct: 301 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 328

[Close]

BLAST of CSPI06G05890 vs. NCBI nr
Match: XP_008460121.1 (PREDICTED: vestitone reductase-like [Cucumis melo] >KAA0039998.1 vestitone reductase-like [Cucumis melo var. makuwa] >TYK24503.1 vestitone reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 649.0 bits (1673), Expect = 2.1e-182
Identity = 317/328 (96.65%), Postives = 327/328 (99.70%), Query Frame = 0


Query: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60
           MES AKGKVCVTGGTGFVASWLIK+LLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL
Sbjct: 1   MESAAKGKVCVTGGTGFVASWLIKKLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60

Query: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK 120
           QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEP+ES+TRRTIEGTLGILKLSVDSK
Sbjct: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPVESLTRRTIEGTLGILKLSVDSK 120

Query: 121 TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS 180
           TVRRVVYTSSAATMQFNHHKVDFLDE+CWSDIDYIN+IAPLGRSYPISKTLTEKAVLEFS
Sbjct: 121 TVRRVVYTSSAATMQFNHHKVDFLDENCWSDIDYINNIAPLGRSYPISKTLTEKAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 240
           QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNE+EYGLILKSNMVHVDDVARAH
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNESEYGLILKSNMVHVDDVARAH 240

Query: 241 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300
           IYLFE+P+ASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL
Sbjct: 241 IYLFEHPSASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300

Query: 301 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           LDAGFQYKYG+EEMLDGAI+SCKEKGYL
Sbjct: 301 LDAGFQYKYGIEEMLDGAIESCKEKGYL 328

[Close]

BLAST of CSPI06G05890 vs. NCBI nr
Match: KGN46250.2 (hypothetical protein Csa_004895 [Cucumis sativus])

HSP 1 Score: 648.7 bits (1672), Expect = 2.8e-182
Identity = 328/355 (92.39%), Postives = 328/355 (92.39%), Query Frame = 0


Query: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADP------------------- 60
           MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADP                   
Sbjct: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPGIFSFLFFGFCFLFYFLRF 60

Query: 61  --------EKRKDYSFLTNLPGASEKLQIYQADLHDPNSFAPAIAGCIGVFHLATPIDVD 120
                   EKRKDYSFLTNLPGASEKLQIYQADLHDPNSFAPAIAGCIGVFHLATPIDVD
Sbjct: 61  SWLRLSDAEKRKDYSFLTNLPGASEKLQIYQADLHDPNSFAPAIAGCIGVFHLATPIDVD 120

Query: 121 DKEPLESVTRRTIEGTLGILKLSVDSKTVRRVVYTSSAATMQFNHHKVDFLDESCWSDID 180
           DKEPLESVTRRTIEGTLGILKLSVDSKTVRRVVYTSSAATMQFNHHKVDFLDESCWSDID
Sbjct: 121 DKEPLESVTRRTIEGTLGILKLSVDSKTVRRVVYTSSAATMQFNHHKVDFLDESCWSDID 180

Query: 181 YINSIAPLGRSYPISKTLTEKAVLEFSQQYGLEVVTVLPTYVVGPFICPKIPGSVHVILS 240
           YINSIAPLGRSYPISKTLTEKAVLEFSQQYGLEVVTVLPTYVVGPFICPKIPGSVHVILS
Sbjct: 181 YINSIAPLGRSYPISKTLTEKAVLEFSQQYGLEVVTVLPTYVVGPFICPKIPGSVHVILS 240

Query: 241 LILGNETEYGLILKSNMVHVDDVARAHIYLFENPNASGRYVCSSHIITLEELANFFSAKY 300
           LILGNETEYGLILKSNMVHVDDVARAHIYLFENPNASGRYVCSSHIITLEELANFFSAKY
Sbjct: 241 LILGNETEYGLILKSNMVHVDDVARAHIYLFENPNASGRYVCSSHIITLEELANFFSAKY 300

Query: 301 PEFQIPSPESLKDVKGYIFTDVSSKKLLDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           PEFQIPSPESLKDVKGYIFTDVSSKKLLDAGFQYKYGVEEMLDGAIQSCKEKGYL
Sbjct: 301 PEFQIPSPESLKDVKGYIFTDVSSKKLLDAGFQYKYGVEEMLDGAIQSCKEKGYL 355

[Close]

BLAST of CSPI06G05890 vs. NCBI nr
Match: XP_038906732.1 (vestitone reductase-like [Benincasa hispida])

HSP 1 Score: 614.4 bits (1583), Expect = 5.8e-172
Identity = 300/328 (91.46%), Postives = 313/328 (95.43%), Query Frame = 0


Query: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60
           MES AKGKVCVTGGTGF+ASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLP ASEKL
Sbjct: 1   MESGAKGKVCVTGGTGFIASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPSASEKL 60

Query: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK 120
           QIY ADL+DPNSFAPAIAGCIGVFHLATPIDV DKEP+E +TRRTIEGT+GILKL +DSK
Sbjct: 61  QIYHADLNDPNSFAPAIAGCIGVFHLATPIDVHDKEPVELLTRRTIEGTMGILKLCLDSK 120

Query: 121 TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS 180
           TVRRVVYTSSAATMQF+HH VDFLDESCWSDI YINSIAP GRSYPISKTLTEKAVLEFS
Sbjct: 121 TVRRVVYTSSAATMQFSHHSVDFLDESCWSDIGYINSIAPYGRSYPISKTLTEKAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 240
           QQYGLEVVTVLPTYVVG FICPKIPGSVHV +SLILGNE EYGLILKSNMVHVDDVARAH
Sbjct: 181 QQYGLEVVTVLPTYVVGSFICPKIPGSVHVTMSLILGNEAEYGLILKSNMVHVDDVARAH 240

Query: 241 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300
           I+LFE+PNA+GRYVCSSHIITLEELANF SAKYPEFQIPSPESLKDVKGY+FTDVSSKKL
Sbjct: 241 IFLFEHPNANGRYVCSSHIITLEELANFLSAKYPEFQIPSPESLKDVKGYVFTDVSSKKL 300

Query: 301 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           LDAGFQYKY +EEM DGAIQSCKEKGYL
Sbjct: 301 LDAGFQYKYSIEEMFDGAIQSCKEKGYL 328

[Close]

BLAST of CSPI06G05890 vs. NCBI nr
Match: XP_023006100.1 (vestitone reductase-like [Cucurbita maxima])

HSP 1 Score: 593.6 bits (1529), Expect = 1.1e-165
Identity = 286/328 (87.20%), Postives = 309/328 (94.21%), Query Frame = 0


Query: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60
           ME+ AKGKVCVTGGTGF+ASWLIK LLENGYSVTTTVRADPE R+DY FLTNLPGASEKL
Sbjct: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60

Query: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK 120
           QIY ADL DPNSFAPAIAGCIGVFHLATPID+ DKE +E +TRRTIEGTLGILK+ +DSK
Sbjct: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120

Query: 121 TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS 180
           TVRRVVYTS+AATMQFNHH VDFLDESCWSDIDYINSIAPLGRSYPISKTLTE+AVLEFS
Sbjct: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 240
           QQYGLEVVTVLPTYVVGPFICP++P S+ V  SL+ GN+ EYGLILKSNMVHVDDVAR+H
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240

Query: 241 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300
           I+LFE+PNA+GRYVCSSH+IT+EELAN FSAKYPEFQIPSPESLKDVKGYIF+DVSSKKL
Sbjct: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300

Query: 301 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           LDAGFQYKYG+EEM DGAIQSCKEKGYL
Sbjct: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYL 328

[Close]

BLAST of CSPI06G05890 vs. TAIR 10
Match: AT2G45400.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 7.5e-85
Identity = 166/328 (50.61%), Postives = 221/328 (67.38%), Query Frame = 0


Query: 7   GKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPE-KRKDYSFLTNLPGASEKLQIYQA 66
           G VCVTGG+GFVASWLI RLL+ GYSV  TVR + E  +KD S+LT LP ASE+LQI+ A
Sbjct: 38  GLVCVTGGSGFVASWLIMRLLQRGYSVRATVRTNSEGNKKDISYLTELPFASERLQIFTA 97

Query: 67  DLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSKTVRRV 126
           DL++P SF PAI GC  VFH+A P+D +  E  E+VT+RT++G +GILK  +D+KTV+R 
Sbjct: 98  DLNEPESFKPAIEGCKAVFHVAHPMDPNSNETEETVTKRTVQGLMGILKSCLDAKTVKRF 157

Query: 127 VYTSSAATMQF---NHHKVDFLDESCWSDIDYINSIAP--LGRSYPISKTLTEKAVLEFS 186
            YTSSA T+ +   N      +DES WSD++   +     +  SY +SK   E A LEF 
Sbjct: 158 FYTSSAVTVFYSGGNGGGGGEVDESVWSDVEVFRNQKEKRVSSSYVVSKMAAETAALEFG 217

Query: 187 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 246
            + GLEVVT++   VVGPFI   +P SV + L+++ GN  E  L    NMVH+DDVARA 
Sbjct: 218 GKNGLEVVTLVIPLVVGPFISSSLPSSVFISLAMLFGNYKEKYLFDTYNMVHIDDVARAM 277

Query: 247 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 306
           I+L E P A GRY+CSS  + ++E+  F S K+P+FQ+PS + L   K      +SSKKL
Sbjct: 278 IFLLEKPVAKGRYICSSVEMKIDEVFEFLSTKFPQFQLPSID-LNKYKVEKRMGLSSKKL 337

Query: 307 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
             AGF++KYG EE+  GAI+SC+ +G+L
Sbjct: 338 KSAGFEFKYGAEEIFSGAIRSCQARGFL 364

[Close]

BLAST of CSPI06G05890 vs. TAIR 10
Match: AT5G42800.1 (dihydroflavonol 4-reductase )

HSP 1 Score: 297.4 bits (760), Expect = 1.5e-80
Identity = 149/328 (45.43%), Postives = 209/328 (63.72%), Query Frame = 0


Query: 6   KGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKLQIYQA 65
           K  VCVTG +GF+ SWL+ RLLE GY V  TVR DP   K    L +LP A   L +++A
Sbjct: 5   KETVCVTGASGFIGSWLVMRLLERGYFVRATVR-DPGNLKKVQHLLDLPNAKTLLTLWKA 64

Query: 66  DLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSKTVRRV 125
           DL +  S+  AI GC GVFH+ATP+D + K+P   V + T+ G LGI+K  V +KTVRR 
Sbjct: 65  DLSEEGSYDDAINGCDGVFHVATPMDFESKDPENEVIKPTVNGMLGIMKACVKAKTVRRF 124

Query: 126 VYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFSQQYGL 185
           V+TSSA T+    H+ +  DE+ WSD+++I S    G  Y +SKTL EKA  +F+++ GL
Sbjct: 125 VFTSSAGTVNVEEHQKNVYDENDWSDLEFIMSKKMTGWMYFVSKTLAEKAAWDFAEEKGL 184

Query: 186 EVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAHIYLFE 245
           + ++++PT VVGPFI   +P S+   LS I  NE  Y +I +   VH+DD+  AHI+L+E
Sbjct: 185 DFISIIPTLVVGPFITTSMPPSLITALSPITRNEAHYSIIRQGQYVHLDDLCNAHIFLYE 244

Query: 246 NPNASGRYVCSSHIITLEELANFFSAKYPEFQIPS-----PESLKDVKGYIFTDVSSKKL 305
              A GRY+CSSH  T+  ++ F   KYPE+ +PS      E+LK +      + SSKKL
Sbjct: 245 QAAAKGRYICSSHDATILTISKFLRPKYPEYNVPSTFEGVDENLKSI------EFSSKKL 304

Query: 306 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
            D GF +KY +EEM   +I++C++KG+L
Sbjct: 305 TDMGFNFKYSLEEMFIESIETCRQKGFL 325

[Close]

BLAST of CSPI06G05890 vs. TAIR 10
Match: AT4G35420.1 (dihydroflavonol 4-reductase-like1 )

HSP 1 Score: 248.4 bits (633), Expect = 7.8e-66
Identity = 139/329 (42.25%), Postives = 200/329 (60.79%), Query Frame = 0


Query: 5   AKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKLQIYQ 64
           AKGKVCVTG +GF+ASWL+KRLL  GY V  TVR DP   K  + L  L GA E+L++ +
Sbjct: 4   AKGKVCVTGASGFLASWLVKRLLLEGYEVIGTVR-DPGNEKKLAHLWKLEGAKERLRLVK 63

Query: 65  ADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSKTVRR 124
           ADL +  SF  AI GC GVFH A+P+      P E + R  IEGTL +L+    + +++R
Sbjct: 64  ADLMEEGSFDNAIMGCQGVFHTASPVLKPTSNPEEEILRPAIEGTLNVLRSCRKNPSLKR 123

Query: 125 VVYTSSAATMQFNHHKVDF-----LDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEF 184
           VV TSS++T++    + DF     LDES W+ ++           Y +SKTL E+A  +F
Sbjct: 124 VVLTSSSSTVRI---RDDFDPKIPLDESIWTSVELCKRFQVW---YALSKTLAEQAAWKF 183

Query: 185 SQQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARA 244
           S++ G+++VTVLP+++VGP + P +  +   +L L+ G   ++    +   VH+DDVAR 
Sbjct: 184 SEENGIDLVTVLPSFLVGPSLPPDLCSTASDVLGLLKGETEKFQWHGQMGYVHIDDVART 243

Query: 245 HIYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKK 304
           HI +FE+  A GRY+CSS++I+LEEL +F SA+YP   IP      +   Y   D  + K
Sbjct: 244 HIVVFEHEAAQGRYICSSNVISLEELVSFLSARYPSLPIPKRFEKLNRLHY---DFDTSK 303

Query: 305 LLDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           +   G ++K  +EEM D  I S  E+GYL
Sbjct: 304 IQSLGLKFK-SLEEMFDDCIASLVEQGYL 321

[Close]

BLAST of CSPI06G05890 vs. TAIR 10
Match: AT1G61720.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.7e-60
Identity = 126/332 (37.95%), Postives = 198/332 (59.64%), Query Frame = 0


Query: 8   KVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKLQIYQADL 67
           K CV GGTG +AS LIK LL++GY V TTVR DPE  K  + L  L    + L+I++ADL
Sbjct: 12  KACVIGGTGNLASILIKHLLQSGYKVNTTVR-DPENEKKIAHLRKLQELGD-LKIFKADL 71

Query: 68  HDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSKTVRRVVY 127
            D +SF  + +GC  +FH+ATPI+   ++P + + +  I+G + +LK  + SK+V+RV+Y
Sbjct: 72  TDEDSFESSFSGCEYIFHVATPINFKSEDPEKDMIKPAIQGVINVLKSCLKSKSVKRVIY 131

Query: 128 TSSAATMQFNHHKVD--FLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFSQQYGL 187
           TSSAA +  N+       ++E  W+D++++    P    YPISK L EK   EF+++  +
Sbjct: 132 TSSAAAVSINNLSGTGIVMNEENWTDVEFLTEEKPFNWGYPISKVLAEKTAWEFAKENKI 191

Query: 188 EVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETE-------YGLILKSNMVHVDDVAR 247
            +VTV+P  + G  +    P S+ + +S I G E           L    + VHVDD+AR
Sbjct: 192 NLVTVIPALIAGNSLLSDPPSSLSLSMSFITGKEMHVTGLKEMQKLSGSISFVHVDDLAR 251

Query: 248 AHIYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPS--PESLKDVKGYIFTDVS 307
           AH++L E   ASGRY+C ++  ++ E+A+F   +YP++ + S   E L   K      +S
Sbjct: 252 AHLFLAEKETASGRYICCAYNTSVPEIADFLIQRYPKYNVLSEFEEGLSIPK----LTLS 311

Query: 308 SKKLLDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           S+KL++ GF+++YG+ EM D  I+  + KG +
Sbjct: 312 SQKLINEGFRFEYGINEMYDQMIEYFESKGLI 337

[Close]

BLAST of CSPI06G05890 vs. TAIR 10
Match: AT2G02400.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 211.8 bits (538), Expect = 8.1e-55
Identity = 125/330 (37.88%), Postives = 197/330 (59.70%), Query Frame = 0


Query: 4   VAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKLQIY 63
           +AK  VCVTG  GF+ SW+I+ L+E GY   T + A      D + L  LPG+  K++I+
Sbjct: 1   MAKETVCVTGANGFIGSWIIRTLIEKGY---TKIHASIYPGSDPTHLLQLPGSDSKIKIF 60

Query: 64  QADLHDPNSFAPAIAGCIGVFHLATPIDVDDK-EPLESVTRRTIEGTLGILKLSVDSKTV 123
           +ADL D ++ + AI GC GVFH+A+P  +D   +P + +    ++GT+ +L+ +     V
Sbjct: 61  EADLLDSDAISRAIDGCAGVFHVASPCTLDPPVDPEKELVEPAVKGTINVLE-AAKRFNV 120

Query: 124 RRVVYTSSAATMQFNHH---KVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEF 183
           RRVV TSS + +  N +   KV  +DES WSD+D+  S     + YPISKTL EKA  EF
Sbjct: 121 RRVVITSSISALVPNPNWPEKVP-VDESSWSDLDFCKS---RQKWYPISKTLAEKAAWEF 180

Query: 184 SQQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGN-ETEYGLILKSNMVHVDDVAR 243
           S+++G  +VT+ P+  +GP + P +  S  V+L L+ G+ ET+    L   +VHV DVA+
Sbjct: 181 SEKHGTNIVTIHPSTCLGPLLQPNLNASCAVLLQLLQGSTETQEHHWL--GVVHVKDVAK 240

Query: 244 AHIYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSK 303
            H+ LFE P+ASGR++C++ I    E A   S  +PEF +   +  +   G    + ++K
Sbjct: 241 GHVMLFETPDASGRFLCTNGIYQFSEFAALVSKLFPEFAVHKFDK-ETQPGLTSCNDAAK 300

Query: 304 KLLDAGFQYKYGVEEMLDGAIQSCKEKGYL 329
           +L++ G  +   VE+ +   +QS ++KG+L
Sbjct: 301 RLIELGLVFT-AVEDAVKETVQSLRDKGFL 318

The following BLAST results are available for this feature:

BLAST of CSPI06G05890 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q40316	5.9e-111	58.20	Vestitone reductase OS=Medicago sativa OX=3879 PE=1 SV=1	[more]
O22133	1.1e-83	50.61	Protein BRI1-5 ENHANCED 1 OS=Arabidopsis thaliana OX=3702 GN=BEN1 PE=2 SV=1	[more]
Q5XLY0	1.9e-80	45.29	Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1	[more]
P51110	1.9e-80	46.46	Dihydroflavonol 4-reductase OS=Vitis vinifera OX=29760 GN=DFR PE=1 SV=1	[more]
Q9XES5	9.2e-80	45.17	Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestic...	[more]

BLAST of CSPI06G05890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K9S9	5.3e-187	100.00	Cinnamoyl-CoA reductase 16 OS=Cucumis sativus OX=3659 GN=CCR16 PE=2 SV=1	[more]
A0A5D3DLI4	1.0e-182	96.65	Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3CBT5	1.0e-182	96.65	vestitone reductase-like OS=Cucumis melo OX=3656 GN=LOC103499024 PE=3 SV=1	[more]
A0A6J1KZ90	5.1e-166	87.20	vestitone reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111498944 PE=3 SV=1	[more]
A0A6J1H5D7	5.3e-163	85.98	vestitone reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111460207 PE=3 SV=1	[more]

BLAST of CSPI06G05890 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145001.1	1.1e-186	100.00	vestitone reductase [Cucumis sativus] >QHJ91613.1 cinnamoyl-CoA reductase 16 [Cu...	[more]
XP_008460121.1	2.1e-182	96.65	PREDICTED: vestitone reductase-like [Cucumis melo] >KAA0039998.1 vestitone reduc...	[more]
KGN46250.2	2.8e-182	92.39	hypothetical protein Csa_004895 [Cucumis sativus]	[more]
XP_038906732.1	5.8e-172	91.46	vestitone reductase-like [Benincasa hispida]	[more]
XP_023006100.1	1.1e-165	87.20	vestitone reductase-like [Cucurbita maxima]	[more]

BLAST of CSPI06G05890 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45400.1	7.5e-85	50.61	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G42800.1	1.5e-80	45.43	dihydroflavonol 4-reductase	[more]
AT4G35420.1	7.8e-66	42.25	dihydroflavonol 4-reductase-like1	[more]
AT1G61720.1	1.7e-60	37.95	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G02400.1	8.1e-55	37.88	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001509	NAD-dependent epimerase/dehydratase	PFAM	PF01370	Epimerase	coord: 9..250 e-value: 6.5E-24 score: 84.7
None	No IPR available	GENE3D	3.40.50.720		coord: 6..328 e-value: 7.8E-121 score: 405.5
None	No IPR available	PANTHER	PTHR10366	NAD DEPENDENT EPIMERASE/DEHYDRATASE	coord: 7..320
None	No IPR available	PANTHER	PTHR10366:SF563	VESTITONE REDUCTASE-LIKE	coord: 7..320
None	No IPR available	CDD	cd08958	FR_SDR_e	coord: 9..305 e-value: 9.5319E-139 score: 393.093
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 4..323

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G05890.1	CSPI06G05890.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
molecular_function	GO:0003824	catalytic activity