CSPI06G05810 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G05810
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	calmodulin-like protein 7
Location	Chr6: 5196446 .. 5197472 (-)
RNA-Seq Expression	CSPI06G05810
Synteny	CSPI06G05810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTAAGAGCCGTAGGACTTTAAACTTCTCTTCTACTTACATAAACTGTTTCTTTTCACAATTTCTCTTATAGAAAAATTTCACTTCTCTTAGTTATGTTCAATCTTACCTCTTCCTTATTCCCTACTTTCTTCCATAGGAATATTTCCTCAAAACCCTTCTTCCAAAAATCCCTAAACAACCAAAAGTTACCATCTTTCACCACTTTTAACCACTTCCAGCTCCTCTTCAACCTCCTCGACTCCGATGGTGACGGCAAGATCTCGATGAAAGAGCTCAGTCAATTTCTCTATCGTCTAGGATATAAAAAGCTGAAAGCTACAATGGAAGCTGAAGAAATGGTGAAGGAAATGGATTCAGACCGTGATGGATTCATTGAAATGGATGAATTTTTAGAGTGAGTATGACTTACATATTAGTTTAAGCACTTTTTATAAAACGTGCTTCAACTTTTGATCTAACATAGACCATTTAAATACTACTTTGATTTCATTTTAATCTTTATACTTTCAAATTTGGCTTTTTTTTTTTTTTTGACTGTACTTGTATATTTTGGAAAATTTAGAGAGACGACTGGGTTAGGCGTAACGGATGGTAAACACATTCTTAGCTTTTAATTTTGATGACGAAACGTTTTAATATAGGGTTTTGCAGAAGGGGGAGGAAGAAGAAGAAATTCTTAGGGAAGCTTTTCTGATATTTGATGGTGATAAGAATGGATTGATTTGTGCTGAGAAATTAAAGAGAACATTGAGCAAATTTGGATGGAAAAAATGTGACCTCAAACAATGTAAAAAGATGATTGAAGGAGTTGATCATGATGGCGATGGCTTTGTGAATTTTCAAGACTTTCGGCTCATGATGACACAGAAAAGATAATCATATATATAATATAGAGATTATTATCTGTAATTTTATATTTGATTTATCTCTAAGAAATGTAATGTCGTCTTTAATTAATGCTTATGATTATGACTTGTAATAATATATCTTCTTTTTCTTTTCTTTTTTTAAAAATTGTAATGGG

mRNA sequence

AATTAAGAGCCGTAGGACTTTAAACTTCTCTTCTACTTACATAAACTGTTTCTTTTCACAATTTCTCTTATAGAAAAATTTCACTTCTCTTAGTTATGTTCAATCTTACCTCTTCCTTATTCCCTACTTTCTTCCATAGGAATATTTCCTCAAAACCCTTCTTCCAAAAATCCCTAAACAACCAAAAGTTACCATCTTTCACCACTTTTAACCACTTCCAGCTCCTCTTCAACCTCCTCGACTCCGATGGTGACGGCAAGATCTCGATGAAAGAGCTCAGTCAATTTCTCTATCGTCTAGGATATAAAAAGCTGAAAGCTACAATGGAAGCTGAAGAAATGGTGAAGGAAATGGATTCAGACCGTGATGGATTCATTGAAATGGATGAATTTTTAGAGGTTTTGCAGAAGGGGGAGGAAGAAGAAGAAATTCTTAGGGAAGCTTTTCTGATATTTGATGGTGATAAGAATGGATTGATTTGTGCTGAGAAATTAAAGAGAACATTGAGCAAATTTGGATGGAAAAAATGTGACCTCAAACAATGTAAAAAGATGATTGAAGGAGTTGATCATGATGGCGATGGCTTTGTGAATTTTCAAGACTTTCGGCTCATGATGACACAGAAAAGATAATCATATATATAATATAGAGATTATTATCTGTAATTTTATATTTGATTTATCTCTAAGAAATGTAATGTCGTCTTTAATTAATGCTTATGATTATGACTTGTAATAATATATCTTCTTTTTCTTTTCTTTTTTTAAAAATTGTAATGGG

Coding sequence (CDS)

ATGTTCAATCTTACCTCTTCCTTATTCCCTACTTTCTTCCATAGGAATATTTCCTCAAAACCCTTCTTCCAAAAATCCCTAAACAACCAAAAGTTACCATCTTTCACCACTTTTAACCACTTCCAGCTCCTCTTCAACCTCCTCGACTCCGATGGTGACGGCAAGATCTCGATGAAAGAGCTCAGTCAATTTCTCTATCGTCTAGGATATAAAAAGCTGAAAGCTACAATGGAAGCTGAAGAAATGGTGAAGGAAATGGATTCAGACCGTGATGGATTCATTGAAATGGATGAATTTTTAGAGGTTTTGCAGAAGGGGGAGGAAGAAGAAGAAATTCTTAGGGAAGCTTTTCTGATATTTGATGGTGATAAGAATGGATTGATTTGTGCTGAGAAATTAAAGAGAACATTGAGCAAATTTGGATGGAAAAAATGTGACCTCAAACAATGTAAAAAGATGATTGAAGGAGTTGATCATGATGGCGATGGCTTTGTGAATTTTCAAGACTTTCGGCTCATGATGACACAGAAAAGATAA

Protein sequence

MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR*

Homology

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BLAST of CSPI06G05810 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.3e-22
Identity = 59/161 (36.65%), Postives = 97/161 (60.25%), Query Frame = 0


Query: 18  SSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATM 77
           +  P+ QK+ + +     T     + +F   D +GDGKIS KEL   +  LG++  +   
Sbjct: 20  ADSPYLQKARSGK-----TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEE-- 79

Query: 78  EAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEI---LREAFLIFDGDKNGLICAEKLK 137
           E E+ + E+D   DG+I  +EF+E+  KG ++ ++   L++AF ++D D NG I AE+L 
Sbjct: 80  ELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELH 139

Query: 138 RTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
             L   G  +C + +C+KMI GVD DGDG ++F++F++MMT
Sbjct: 140 EVLRSLG-DECSIAECRKMIGGVDKDGDGTIDFEEFKIMMT 172

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BLAST of CSPI06G05810 vs. ExPASy Swiss-Prot
Match: P14533 (Squidulin OS=Doryteuthis pealeii OX=1051067 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 5.3e-21
Identity = 56/136 (41.18%), Postives = 87/136 (63.97%), Query Frame = 0


Query: 45  FNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQ 104
           F++ D DGDG+I+ KEL   +  LG  +  +  E EEM++E+D+D +G IE  EF+E++ 
Sbjct: 16  FDMFDIDGDGQITSKELRSVMKSLG--RTPSDAELEEMIREVDTDGNGTIEYAEFVEMMA 75

Query: 105 K---GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDG 164
           K     + E+ +REAF +FD D NGLI A +L++ ++ F  +K   ++  +MI   D DG
Sbjct: 76  KQMGPTDPEKEMREAFRVFDKDGNGLITAAELRQVMANFSDEKLTSEEISEMIREADIDG 135

Query: 165 DGFVNFQDFRLMMTQK 178
           DG VN+++F  MMT K
Sbjct: 136 DGMVNYEEFVKMMTPK 149

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BLAST of CSPI06G05810 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 9.0e-21
Identity = 56/132 (42.42%), Postives = 84/132 (63.64%), Query Frame = 0


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVL 103
           +F+  DS+GDGKIS+ EL      +G    +   E   +++E+D+DRDG+I +DEF  + 
Sbjct: 27  VFDQFDSNGDGKISVLELGGVFKAMGTSYTET--ELNRVLEEVDTDRDGYINLDEFSTLC 86

Query: 104 QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDG 163
           +      EI R+AF ++D DKNGLI A +L + L++ G   C ++ C +MI  VD DGDG
Sbjct: 87  RSSSSAAEI-RDAFDLYDQDKNGLISASELHQVLNRLG-MSCSVEDCTRMIGPVDADGDG 146

Query: 164 FVNFQDFRLMMT 176
            VNF++F+ MMT
Sbjct: 147 NVNFEEFQKMMT 154

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BLAST of CSPI06G05810 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.5e-20
Identity = 67/172 (38.95%), Postives = 93/172 (54.07%), Query Frame = 0


Query: 10  PTFFHRNISSK--PFFQKS-LNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKELSQFLY 69
           P  F R   SK  P  Q    +    P+ T     + +F   D++GDG+IS  EL     
Sbjct: 7   PALFRRRSGSKSPPLPQADPASGGGSPAPTPEEEMERVFRKFDANGDGRISRSELGALFE 66

Query: 70  RLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEF--LEVLQKGEEE--EEILREAFLIFDG 129
            LG+       E   M+ E D+D DGFI +DEF  L     G+    EE LR AF +FD 
Sbjct: 67  SLGH--AATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDA 126

Query: 130 DKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMM 175
           D NG I A +L R L   G +K  ++QC++MIEGVD +GDG ++F++F++MM
Sbjct: 127 DGNGTISAAELARVLHGLG-EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

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BLAST of CSPI06G05810 vs. ExPASy Swiss-Prot
Match: P11121 (Calmodulin OS=Pyuridae sp. OX=7734 PE=1 SV=2)

HSP 1 Score: 100.5 bits (249), Expect = 2.0e-20
Identity = 58/140 (41.43%), Postives = 84/140 (60.00%), Query Frame = 0


Query: 41  FQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFL 100
           F+  F+L D DGDG I+ KEL   +  LG    +A  E ++M+ E+D+D DG I+  EFL
Sbjct: 13  FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA--ELQDMINEVDADGDGTIDFPEFL 72

Query: 101 EVL---QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGV 160
            ++    K  + EE +REAF +FD D NG I A +L+  ++  G K  D ++  +MI   
Sbjct: 73  TMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTD-EEVDEMIREA 132

Query: 161 DHDGDGFVNFQDFRLMMTQK 178
           D DGDG VN+++F  MMT K
Sbjct: 133 DIDGDGQVNYEEFVTMMTSK 149

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BLAST of CSPI06G05810 vs. ExPASy TrEMBL
Match: A0A0A0K8Z4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G076830 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 3.7e-94
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKE 60
           MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKIS KE
Sbjct: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISTKE 60

Query: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120
           LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF
Sbjct: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120

Query: 121 DGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
           DGDKNGLICAEKLKRTLSKFGW+KCDLKQCKKMIEGVDHDGDG+VNFQDFRLMMTQKR
Sbjct: 121 DGDKNGLICAEKLKRTLSKFGWQKCDLKQCKKMIEGVDHDGDGYVNFQDFRLMMTQKR 178

[Close]

BLAST of CSPI06G05810 vs. ExPASy TrEMBL
Match: A0A1S3CBX8 (calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103499032 PE=4 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 9.5e-90
Identity = 168/178 (94.38%), Postives = 171/178 (96.07%), Query Frame = 0


Query: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKE 60
           M NLTSSLF TFFHRNI+SKPFFQKSLNNQKLPSFTTFNHFQLLFN+LDS GDGKIS KE
Sbjct: 1   MLNLTSSLFSTFFHRNITSKPFFQKSLNNQKLPSFTTFNHFQLLFNILDSGGDGKISTKE 60

Query: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120
           LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF
Sbjct: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120

Query: 121 DGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
           DGDKNGLICAE+LKRT  KFGW KCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR
Sbjct: 121 DGDKNGLICAEELKRTFRKFGWTKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 178

[Close]

BLAST of CSPI06G05810 vs. ExPASy TrEMBL
Match: A0A5A7TEW8 (Calmodulin-like protein 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold122G002880 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.0e-43
Identity = 95/101 (94.06%), Postives = 96/101 (95.05%), Query Frame = 0


Query: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKE 60
           M NLTSSLF TFFHRNI+SKPFFQKSLNNQKLPSFTT NHFQLLFNLLDSDGDGKIS KE
Sbjct: 1   MLNLTSSLFSTFFHRNITSKPFFQKSLNNQKLPSFTTSNHFQLLFNLLDSDGDGKISTKE 60

Query: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLE 102
           LSQFLYRLGYKKLK TMEAEEMVKEMDSDRDGFIEMDEFLE
Sbjct: 61  LSQFLYRLGYKKLKPTMEAEEMVKEMDSDRDGFIEMDEFLE 101

[Close]

BLAST of CSPI06G05810 vs. ExPASy TrEMBL
Match: A0A067JXS4 (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_14512 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.9e-34
Identity = 83/164 (50.61%), Postives = 115/164 (70.12%), Query Frame = 0


Query: 15  RNISSKPFFQKSLNNQKLPSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYK 74
           RN+SS  F     +    PSF +    N F+ +FNL+D++GDGKIS  +L + L  LG  
Sbjct: 25  RNLSSSTF-----DFSDTPSFASMEIPNQFKQVFNLIDANGDGKISSYDLIELLLCLGCD 84

Query: 75  KLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIFDGDKNGLICAE 134
           K KA  EAE MVKE+D + DGF++ DEF  +L    +EE+ L +AF+IFD DKNGLI A+
Sbjct: 85  KSKAKAEAEGMVKELDRNGDGFVDFDEFFYIL-NNYDEEDYLMDAFMIFDADKNGLISAK 144

Query: 135 KLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
           +L++ LS+ G++KC LK+C+ MI+GVD DGDGFV+F++FR MMT
Sbjct: 145 ELQKVLSRLGFEKCSLKECRIMIKGVDKDGDGFVDFEEFRSMMT 182

[Close]

BLAST of CSPI06G05810 vs. ExPASy TrEMBL
Match: B9RDE4 (Calmodulin, putative OS=Ricinus communis OX=3988 GN=RCOM_1612330 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 2.5e-34
Identity = 81/154 (52.60%), Postives = 107/154 (69.48%), Query Frame = 0


Query: 33  PSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSD 92
           PSF      N F+ +F L+D++GDGKIS  ELS+ L  LG+ K KAT EAE MV+EMD +
Sbjct: 24  PSFAAMEVHNQFKQVFKLIDANGDGKISSCELSELLLCLGFDKSKATSEAEGMVREMDCN 83

Query: 93  RDGFIEMDEFLEVLQK--------GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFG 152
            DGF++MDEF+  L          G+ +++ L +AFLIFD DKNGLI AE+LK+ L+  G
Sbjct: 84  GDGFVDMDEFIYTLNDDGKLRGVGGDNKKDYLMDAFLIFDADKNGLISAEELKKVLTNLG 143

Query: 153 WKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
              C LK+C++MI+GVD DGDG VNF++FR MMT
Sbjct: 144 CDNCSLKKCRRMIKGVDKDGDGSVNFEEFRSMMT 177

[Close]

BLAST of CSPI06G05810 vs. NCBI nr
Match: XP_004153117.1 (calmodulin-beta [Cucumis sativus] >KGN46235.1 hypothetical protein Csa_005220 [Cucumis sativus])

HSP 1 Score: 354.0 bits (907), Expect = 7.7e-94
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKE 60
           MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKIS KE
Sbjct: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISTKE 60

Query: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120
           LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF
Sbjct: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120

Query: 121 DGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
           DGDKNGLICAEKLKRTLSKFGW+KCDLKQCKKMIEGVDHDGDG+VNFQDFRLMMTQKR
Sbjct: 121 DGDKNGLICAEKLKRTLSKFGWQKCDLKQCKKMIEGVDHDGDGYVNFQDFRLMMTQKR 178

[Close]

BLAST of CSPI06G05810 vs. NCBI nr
Match: XP_008460131.1 (PREDICTED: calmodulin-like protein 7 [Cucumis melo])

HSP 1 Score: 339.3 bits (869), Expect = 2.0e-89
Identity = 168/178 (94.38%), Postives = 171/178 (96.07%), Query Frame = 0


Query: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKE 60
           M NLTSSLF TFFHRNI+SKPFFQKSLNNQKLPSFTTFNHFQLLFN+LDS GDGKIS KE
Sbjct: 1   MLNLTSSLFSTFFHRNITSKPFFQKSLNNQKLPSFTTFNHFQLLFNILDSGGDGKISTKE 60

Query: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120
           LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF
Sbjct: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120

Query: 121 DGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
           DGDKNGLICAE+LKRT  KFGW KCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR
Sbjct: 121 DGDKNGLICAEELKRTFRKFGWTKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 178

[Close]

BLAST of CSPI06G05810 vs. NCBI nr
Match: KAA0040005.1 (calmodulin-like protein 7 [Cucumis melo var. makuwa])

HSP 1 Score: 186.4 bits (472), Expect = 2.1e-43
Identity = 95/101 (94.06%), Postives = 96/101 (95.05%), Query Frame = 0


Query: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKE 60
           M NLTSSLF TFFHRNI+SKPFFQKSLNNQKLPSFTT NHFQLLFNLLDSDGDGKIS KE
Sbjct: 1   MLNLTSSLFSTFFHRNITSKPFFQKSLNNQKLPSFTTSNHFQLLFNLLDSDGDGKISTKE 60

Query: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLE 102
           LSQFLYRLGYKKLK TMEAEEMVKEMDSDRDGFIEMDEFLE
Sbjct: 61  LSQFLYRLGYKKLKPTMEAEEMVKEMDSDRDGFIEMDEFLE 101

[Close]

BLAST of CSPI06G05810 vs. NCBI nr
Match: XP_012083557.1 (probable calcium-binding protein CML27 [Jatropha curcas] >KDP28741.1 hypothetical protein JCGZ_14512 [Jatropha curcas])

HSP 1 Score: 155.6 bits (392), Expect = 4.0e-34
Identity = 83/164 (50.61%), Postives = 115/164 (70.12%), Query Frame = 0


Query: 15  RNISSKPFFQKSLNNQKLPSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYK 74
           RN+SS  F     +    PSF +    N F+ +FNL+D++GDGKIS  +L + L  LG  
Sbjct: 25  RNLSSSTF-----DFSDTPSFASMEIPNQFKQVFNLIDANGDGKISSYDLIELLLCLGCD 84

Query: 75  KLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIFDGDKNGLICAE 134
           K KA  EAE MVKE+D + DGF++ DEF  +L    +EE+ L +AF+IFD DKNGLI A+
Sbjct: 85  KSKAKAEAEGMVKELDRNGDGFVDFDEFFYIL-NNYDEEDYLMDAFMIFDADKNGLISAK 144

Query: 135 KLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
           +L++ LS+ G++KC LK+C+ MI+GVD DGDGFV+F++FR MMT
Sbjct: 145 ELQKVLSRLGFEKCSLKECRIMIKGVDKDGDGFVDFEEFRSMMT 182

[Close]

BLAST of CSPI06G05810 vs. NCBI nr
Match: EEF50402.1 (calmodulin, putative [Ricinus communis])

HSP 1 Score: 155.2 bits (391), Expect = 5.2e-34
Identity = 81/154 (52.60%), Postives = 107/154 (69.48%), Query Frame = 0


Query: 33  PSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSD 92
           PSF      N F+ +F L+D++GDGKIS  ELS+ L  LG+ K KAT EAE MV+EMD +
Sbjct: 24  PSFAAMEVHNQFKQVFKLIDANGDGKISSCELSELLLCLGFDKSKATSEAEGMVREMDCN 83

Query: 93  RDGFIEMDEFLEVLQK--------GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFG 152
            DGF++MDEF+  L          G+ +++ L +AFLIFD DKNGLI AE+LK+ L+  G
Sbjct: 84  GDGFVDMDEFIYTLNDDGKLRGVGGDNKKDYLMDAFLIFDADKNGLISAEELKKVLTNLG 143

Query: 153 WKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
              C LK+C++MI+GVD DGDG VNF++FR MMT
Sbjct: 144 CDNCSLKKCRRMIKGVDKDGDGSVNFEEFRSMMT 177

[Close]

BLAST of CSPI06G05810 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 107.8 bits (268), Expect = 8.9e-24
Identity = 59/161 (36.65%), Postives = 97/161 (60.25%), Query Frame = 0


Query: 18  SSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATM 77
           +  P+ QK+ + +     T     + +F   D +GDGKIS KEL   +  LG++  +   
Sbjct: 20  ADSPYLQKARSGK-----TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEE-- 79

Query: 78  EAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEI---LREAFLIFDGDKNGLICAEKLK 137
           E E+ + E+D   DG+I  +EF+E+  KG ++ ++   L++AF ++D D NG I AE+L 
Sbjct: 80  ELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELH 139

Query: 138 RTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
             L   G  +C + +C+KMI GVD DGDG ++F++F++MMT
Sbjct: 140 EVLRSLG-DECSIAECRKMIGGVDKDGDGTIDFEEFKIMMT 172

[Close]

BLAST of CSPI06G05810 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 101.7 bits (252), Expect = 6.4e-22
Identity = 56/132 (42.42%), Postives = 84/132 (63.64%), Query Frame = 0


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVL 103
           +F+  DS+GDGKIS+ EL      +G    +   E   +++E+D+DRDG+I +DEF  + 
Sbjct: 27  VFDQFDSNGDGKISVLELGGVFKAMGTSYTET--ELNRVLEEVDTDRDGYINLDEFSTLC 86

Query: 104 QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDG 163
           +      EI R+AF ++D DKNGLI A +L + L++ G   C ++ C +MI  VD DGDG
Sbjct: 87  RSSSSAAEI-RDAFDLYDQDKNGLISASELHQVLNRLG-MSCSVEDCTRMIGPVDADGDG 146

Query: 164 FVNFQDFRLMMT 176
            VNF++F+ MMT
Sbjct: 147 NVNFEEFQKMMT 154

[Close]

BLAST of CSPI06G05810 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 101.7 bits (252), Expect = 6.4e-22
Identity = 56/132 (42.42%), Postives = 84/132 (63.64%), Query Frame = 0


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVL 103
           +F+  DS+GDGKIS+ EL      +G    +   E   +++E+D+DRDG+I +DEF  + 
Sbjct: 27  VFDQFDSNGDGKISVLELGGVFKAMGTSYTET--ELNRVLEEVDTDRDGYINLDEFSTLC 86

Query: 104 QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDG 163
           +      EI R+AF ++D DKNGLI A +L + L++ G   C ++ C +MI  VD DGDG
Sbjct: 87  RSSSSAAEI-RDAFDLYDQDKNGLISASELHQVLNRLG-MSCSVEDCTRMIGPVDADGDG 146

Query: 164 FVNFQDFRLMMT 176
            VNF++F+ MMT
Sbjct: 147 NVNFEEFQKMMT 154

[Close]

BLAST of CSPI06G05810 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 98.6 bits (244), Expect = 5.4e-21
Identity = 57/135 (42.22%), Postives = 84/135 (62.22%), Query Frame = 0


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVL 103
           +F + D +GDGKI+  EL  F   +G   +    E  EM+ +MD + DG +++DEF  + 
Sbjct: 9   VFQMFDKNGDGKIAKNELKDFFKSVGI--MVPENEINEMIAKMDVNGDGAMDIDEFGSLY 68

Query: 104 Q---KGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKK-CDLKQCKKMIEGVDH 163
           Q   + +EEEE +REAF +FD + +G I  E+L+  L+  G K+   L+ CKKMI  VD 
Sbjct: 69  QEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDV 128

Query: 164 DGDGFVNFQDFRLMM 175
           DGDG VNF++F+ MM
Sbjct: 129 DGDGMVNFKEFKQMM 141

[Close]

BLAST of CSPI06G05810 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 97.8 bits (242), Expect = 9.2e-21
Identity = 59/150 (39.33%), Postives = 81/150 (54.00%), Query Frame = 0


Query: 31  KLPSFTTFNHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDR 90
           ++PS  ++      F L+D D DG +S  +L   L RLG   L    E   M+KE+D D 
Sbjct: 61  EVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPL-TEEEINVMLKEVDCDG 120

Query: 91  DGFIEMDEFLE--VLQKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLK 150
           DG I ++E     V      +   L+E F  FD D++GLI A++L R  S  G ++C L 
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLD 180

Query: 151 QCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
            CK+MI  VD DGDGFV F +F  MM  +R
Sbjct: 181 DCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

The following BLAST results are available for this feature:

BLAST of CSPI06G05810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYK2	1.3e-22	36.65	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
P14533	5.3e-21	41.18	Squidulin OS=Doryteuthis pealeii OX=1051067 PE=1 SV=1	[more]
Q9LE22	9.0e-21	42.42	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
Q8RZB5	1.5e-20	38.95	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
P11121	2.0e-20	41.43	Calmodulin OS=Pyuridae sp. OX=7734 PE=1 SV=2	[more]

BLAST of CSPI06G05810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K8Z4	3.7e-94	98.31	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G076830 PE=4 SV=1	[more]
A0A1S3CBX8	9.5e-90	94.38	calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103499032 PE=4 SV=1	[more]
A0A5A7TEW8	1.0e-43	94.06	Calmodulin-like protein 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A067JXS4	1.9e-34	50.61	Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_14512 PE=4 SV=1	[more]
B9RDE4	2.5e-34	52.60	Calmodulin, putative OS=Ricinus communis OX=3988 GN=RCOM_1612330 PE=4 SV=1	[more]

BLAST of CSPI06G05810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004153117.1	7.7e-94	98.31	calmodulin-beta [Cucumis sativus] >KGN46235.1 hypothetical protein Csa_005220 [C...	[more]
XP_008460131.1	2.0e-89	94.38	PREDICTED: calmodulin-like protein 7 [Cucumis melo]	[more]
KAA0040005.1	2.1e-43	94.06	calmodulin-like protein 7 [Cucumis melo var. makuwa]	[more]
XP_012083557.1	4.0e-34	50.61	probable calcium-binding protein CML27 [Jatropha curcas] >KDP28741.1 hypothetica...	[more]
EEF50402.1	5.2e-34	52.60	calmodulin, putative [Ricinus communis]	[more]

BLAST of CSPI06G05810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	8.9e-24	36.65	EF hand calcium-binding protein family	[more]
AT1G18210.1	6.4e-22	42.42	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	6.4e-22	42.42	Calcium-binding EF-hand family protein	[more]
AT4G12860.1	5.4e-21	42.22	EF hand calcium-binding protein family	[more]
AT3G10190.1	9.2e-21	39.33	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 40..68 e-value: 0.0026 score: 27.0 coord: 149..177 e-value: 0.0015 score: 27.8 coord: 78..106 e-value: 0.027 score: 23.6 coord: 112..140 e-value: 0.91 score: 18.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 113..174 e-value: 5.2E-7 score: 30.0 coord: 42..103 e-value: 2.9E-12 score: 46.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 74..109 score: 12.365459
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 145..178 score: 11.584389
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 36..71 score: 13.369694
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 110..143 score: 10.245409
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 40..104 e-value: 2.98644E-12 score: 56.7873
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 113..175 e-value: 2.02201E-11 score: 54.8613
None	No IPR available	GENE3D	1.10.238.10		coord: 108..178 e-value: 1.8E-17 score: 65.3
None	No IPR available	GENE3D	1.10.238.10		coord: 12..107 e-value: 4.0E-17 score: 64.7
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 28..176
None	No IPR available	PANTHER	PTHR23050:SF382	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 28..176
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 87..99
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 158..170
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 49..61
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 37..175

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G05810.1	CSPI06G05810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding