CSPI06G05250 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G05250
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Procollagen-proline 4-dioxygenase
Location	Chr6: 4794120 .. 4798371 (+)
RNA-Seq Expression	CSPI06G05250
Synteny	CSPI06G05250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCTCTATGAATTTTGGAACTTCCATTACATTCCCATCAAATTTCCTCCGATAACTCTCTCTCTCTCTCTCTCCCTCTCGCGCGCGCGCTCTTTCTAATTTGATCCGATCGAGACTATGTTCAAATTTGATAATCTGTTATTCATCTTCTTGATTTTGACCTCATCGTTTATTCGGGAATCAACTTGTTCTTATGCTGGTTCGGCTAGCGCAACCGTAGATCCTAGTAAAGTGAAGCAGATTTCATGGAAACCGAGGTGTTTATTGCTAGCATTTTATAACGAACGGATCTTCTTTTTGTTTTCTTCGTTCTATTGTGTTTGTTTTCTGAGAAAGTTGTGGAACGGAAATTATTCAACTCTAACTTGTTTTAGCTTCTAGCTTCCGGTCGTTTAGGTTTAGTTCAGTGGCTATTGATAGTAATTTATGCTTTTCAAATTCTTGATAAGGCGATTTCACTGAATGTTGCAGAGCTTTTGTATATGAAGGTTTTCTAACGGACCTAGAATGCGACCATCTGGTTTCTATAGTAAGTTTAGATTTAGTGATATTCATTGTTCAGTTTGATATGGAAGTCATTTCGTTTCGTCAATACAAATCTATTGTTGAAGGATTGTTTTTCTTCTGTGACAGGCGAGATCCGAGCTAAAGAGATCTGAGGTTGCTGATAATGATTCGGGAAAGAGTAAGCTCAGTACTGTACGAACGAGTTCAGGAATGTTCATTTCTAAAAACAAGGTTAGGTTGAATCTCCACACTAACAATCTTCTAAGTTTTCTGGATGTTTTCTCTAATTTCTCTTTCGATTTTTGTTTTTGATCGGAATCGAGGTTCTGTTACTAGTCGTCACCGAAGCTATTTATTGAATTAAACTTAATGGAAGAATCTTCTCCGTTGCGAAACTGAAATATATTGCCATTTCAAAGAGATTAAACAATCAAGTTACGGCAGGAATGTTCCTGATAGTATGGATGCAATAATCAACATTACGTTCATACAAAATTAGAGAGTAAACAATCTTTCAGGAATCATAACTTAAAACCTCCTCCTCTTCTATCTTGTTGAATTAGGACTAGATTAACAAGTGTGTATTTTGTGCTCTTCACCTGTATCTTAAAATTGATGTATATAGCAGGACAATAATTATTTCCTTTGGTTCTGTTGAGTATCAGTTGAGATCTTTACACCCATGTTTGTATCGAATATTTACATTTTGATTTGATATTTAGGATCCTATTGTTTCTGGCATAGAGGACAAAATTTCTGCATGGACTTTTCTTCCAAAAGGTACACGTTCACTGTCAGATCACATATTTTGTAATTGATATGTTATGTCATATGCTTTTGCTTTACTAGAGCTGATTGCCATAAGTAACATTCATGGTTTCTTTATAATGAAATGGTTGTCAATGTCGAGTATCACTGCTAAATGCCGTGTGTATTTCTCGTTTTTCAGAAAATGGGGAGGACATTCAGGTATTGAGATATGAGCATGGGCAGAAATATGAGTCACATTATGATTACTTTGTTGACAAGGTGAATATTGCCTGGGGTGGACATCGTTTAGCTACGGTCCTTATGTATCTCTCTAATGTGACCCAAGGTGGTGAAACAGTTTTCCCCTTGGCAGAGGTTAGTTCAAGCGTTAATTCGTACTTTACTAGAATTGCACTTTATATTCCTCATCGTAAGACTACTTCTTCTTAGATCTCAGTACTCTCTAAATAATAATCATACAACTCTGTCTTCTGATGGAGAAGATCCTTTTATAAGACTGATACGTGGAAATATTGAAATGAAACATGAGAAAGACTCGATGTGTTGGATAATCCAGAGGATGTTGCCATTATTTTATAGGAATCACAACTGGAAATGACCTGATTTTTGTTCAGGTTGATGACACCGAACCCTTTTACCCAATCTGAACCATTTTTCTGTTTCTTTTGTTTAATTTTCTTATTTTTTGGATTTTGTTTTTCTATCTAAATGTTTATCAATCGATCTATATTCATTATGGATGCAATACAATCCTGCTTAGAGGGATTTAGCACTTTTTGCTACCATCTTTTTAAGTACTTCAGTTAGGACAAATGTTATTGCAGTCATTTTAAAGCACCGAAATTCACCAATTATCTAAAGATTTGAACCCAACTATTGTTGAATTGGATTAGACATGAAACATCTGAGATTATTGAAGTTGTTCCTTGCTGAGAAGCCAACTTGAAACAAACTTCTATAATTAGTTATGAAGTTACATATAGATTGGAAATGGATTTGATTCAAAAATTACTTTAATCAATAGCAAACCTGTTTAACCTCGTTGTATAACCTCGGAGCAGAAACCTTCCCACCGGAGAGCTTATGAAACAGACGAGGACCTCTCAGAGTGCGCTAAGAAAGGAGTTGCAGGTGAATCACCAAACAAACTATAAATGCTTCTTTTTCGCTACTTATAAAAAACTATAAAACTTTCATGTAATTAATTGATGTACTTTGTTATTTAACATGATTTGCTTGGAATGATGTAGTGAAACCAAAGAAAGGCGATGCCCTTCTTTTCTTTAGCCTTGAACCAAATGCTATACCAGACACAAACAGTCTCCATGGAGGTTGCCCTGTCCTTGAAGGAGAAAAATGGTCAGCAACAAAGTGGATTCACGTAGATTCTTTCAGCAAAAACTTAGGAGACATTGGGAATTGTACTGATCTAAATGAAAGTTGTGAGAGATGGGCTGCCTTAGGGGAATGCACCAAAAACCCAGAATATATGGTTGGATCTCCAGAAATGCCCGGCTACTGTCGGAGGAGTTGCAGGATCTGTTGATTTCATTTCAACCTTACACTCGAAATTCCGATACCTTTTCGTGGTAAACTTCTTACTCCTTGGTTGGATAGTAGTAATCAACTTGTTGCTAAGCTTTCGTTCAAACCATTTGGTTTTTAGTTTTTGAAAATTAAGCCTTTAAATACTTATATCTCGACTAAATTTCTTGTTTTGTAATGATTATTTATCTATATTTTCAAAAACCAAGTCAAATTTTAGAATTTAAAAACAATAGAGTTTTTTAAAAATAACAAAATATGAAACTATTTACGAAATATAACAAAATTGAGAATGATACATACTATTGATTTTTGTTTTCGGAATTTGACAAAGATTTCAATTACCATCGTAAAAAATGAACATGAGTTTTTGAAAATAAAAAAAAACAAATTGGTTACTGGATGAGACTGAATTATTGGCTTTTAAGTTTCGGAATTTCTTTTCATTTGCGTGGCACTTCAATTTGTTTAGGCTTTCCCTGTTACCTACACTCAATTGTCATATGTTTCCTTCCAAAAATGAAATAGCTTAAATTGCAAGTTTAGTACCCATAGTAGGAAAGAAGTTAGAATTCAGCTCATGGCTTATAATGTAAATTTAGTTTTTATGGTAGCACTGTTTATGAAAATTTTATGGACCTATAAAGACTAAAGTCTTAACCTTTAAAATAAGGATTAAATTCCAACTTACTCTAGAATCAATTTTGTAATTTAATCGAAAAATATAACTCTTAGATAAAGCTTTGTCCCTCATGAATTTCTTTACCTTTTTTTTTCTTTTTTCTTTTTTCTTTTTTGGTTTTGGTGTCATTTGGAAAGTCAGTCATTTGTGGAATGGAAAGCGATACAACATTGATTGTGAAGCTATCGTGGAAGCACAGATGGGATGAAGCAGTAGCCGTTGGGTAATTATGACCTTTCCTTTTTTTTAAAAAAAAATTCATGATTATGTATTACTTTATATTCTCGTTATTTTTCTTACTTGAATTTTGATACGGTTTTCTTATAGGAAAGAATATTGTTGTTTTAAAAACTAAAGTTTACATACGTTACAGAAAAAAAGAGTAATTTTGCTGATGCGACAATGAATTGATTAGAGAGTCTAACCACCAGCCTTTCCTTTCAATATGAAAGATATAAAAATACAATCCCTTCACCTTTCAGGAAAAAAGAACAATCATTCTTTTAAATGCTTTCGCTATTTATTCTCTTAGCTCATTTTAGTTTCTTTTTTCTCTATAGGAAAAAATAGTTTCAATTCCTTTTGTTTTTTATTCCTCTATTGAGGAGACAGTGTTCGTAAACGGGTCGATAATCATTTGCATGCAAAGTCAATTGACTCTCTCAAATCTTGAAAACTGCTTTCTTTATATATATTTTTCTCGACCTCATTGTGATTTATCATTTGATTCCATGTCATCTACAAGCA

mRNA sequence

CTTCTCTATGAATTTTGGAACTTCCATTACATTCCCATCAAATTTCCTCCGATAACTCTCTCTCTCTCTCTCTCCCTCTCGCGCGCGCGCTCTTTCTAATTTGATCCGATCGAGACTATGTTCAAATTTGATAATCTGTTATTCATCTTCTTGATTTTGACCTCATCGTTTATTCGGGAATCAACTTGTTCTTATGCTGGTTCGGCTAGCGCAACCGTAGATCCTAGTAAAGTGAAGCAGATTTCATGGAAACCGAGAGCTTTTGTATATGAAGGTTTTCTAACGGACCTAGAATGCGACCATCTGGTTTCTATAGCGAGATCCGAGCTAAAGAGATCTGAGGTTGCTGATAATGATTCGGGAAAGAGTAAGCTCAGTACTGTACGAACGAGTTCAGGAATGTTCATTTCTAAAAACAAGGATCCTATTGTTTCTGGCATAGAGGACAAAATTTCTGCATGGACTTTTCTTCCAAAAGAAAATGGGGAGGACATTCAGGTATTGAGATATGAGCATGGGCAGAAATATGAGTCACATTATGATTACTTTGTTGACAAGGTGAATATTGCCTGGGGTGGACATCGTTTAGCTACGGTCCTTATGTATCTCTCTAATGTGACCCAAGGTGGTGAAACAGTTTTCCCCTTGGCAGAGAAACCTTCCCACCGGAGAGCTTATGAAACAGACGAGGACCTCTCAGAGTGCGCTAAGAAAGGAGTTGCAGTGAAACCAAAGAAAGGCGATGCCCTTCTTTTCTTTAGCCTTGAACCAAATGCTATACCAGACACAAACAGTCTCCATGGAGGTTGCCCTGTCCTTGAAGGAGAAAAATGGTCAGCAACAAAGTGGATTCACGTAGATTCTTTCAGCAAAAACTTAGGAGACATTGGGAATTGTACTGATCTAAATGAAAGTTGTGAGAGATGGGCTGCCTTAGGGGAATGCACCAAAAACCCAGAATATATGGTTGGATCTCCAGAAATGCCCGGCTACTGTCGGAGGAGTTGCAGGATCTGTTGATTTCATTTCAACCTTACACTCGAAATTCCGATACCTTTTCGTGTCAGTCATTTGTGGAATGGAAAGCGATACAACATTGATTGTGAAGCTATCGTGGAAGCACAGATGGGATGAAGCAGTAGCCGTTGGGTAATTATGACCTTTCCTTTTTTTTAAAAAAAAATTCATGATTATGTATTACTTTATATTCTCGTTATTTTTCTTACTTGAATTTTGATACGGTTTTCTTATAGGAAAGAATATTGTTGTTTTAAAAACTAAAGTTTACATACGTTACAGAAAAAAAGAGTAATTTTGCTGATGCGACAATGAATTGATTAGAGAGTCTAACCACCAGCCTTTCCTTTCAATATGAAAGATATAAAAATACAATCCCTTCACCTTTCAGGAAAAAAGAACAATCATTCTTTTAAATGCTTTCGCTATTTATTCTCTTAGCTCATTTTAGTTTCTTTTTTCTCTATAGGAAAAAATAGTTTCAATTCCTTTTGTTTTTTATTCCTCTATTGAGGAGACAGTGTTCGTAAACGGGTCGATAATCATTTGCATGCAAAGTCAATTGACTCTCTCAAATCTTGAAAACTGCTTTCTTTATATATATTTTTCTCGACCTCATTGTGATTTATCATTTGATTCCATGTCATCTACAAGCA

Coding sequence (CDS)

ATGTTCAAATTTGATAATCTGTTATTCATCTTCTTGATTTTGACCTCATCGTTTATTCGGGAATCAACTTGTTCTTATGCTGGTTCGGCTAGCGCAACCGTAGATCCTAGTAAAGTGAAGCAGATTTCATGGAAACCGAGAGCTTTTGTATATGAAGGTTTTCTAACGGACCTAGAATGCGACCATCTGGTTTCTATAGCGAGATCCGAGCTAAAGAGATCTGAGGTTGCTGATAATGATTCGGGAAAGAGTAAGCTCAGTACTGTACGAACGAGTTCAGGAATGTTCATTTCTAAAAACAAGGATCCTATTGTTTCTGGCATAGAGGACAAAATTTCTGCATGGACTTTTCTTCCAAAAGAAAATGGGGAGGACATTCAGGTATTGAGATATGAGCATGGGCAGAAATATGAGTCACATTATGATTACTTTGTTGACAAGGTGAATATTGCCTGGGGTGGACATCGTTTAGCTACGGTCCTTATGTATCTCTCTAATGTGACCCAAGGTGGTGAAACAGTTTTCCCCTTGGCAGAGAAACCTTCCCACCGGAGAGCTTATGAAACAGACGAGGACCTCTCAGAGTGCGCTAAGAAAGGAGTTGCAGTGAAACCAAAGAAAGGCGATGCCCTTCTTTTCTTTAGCCTTGAACCAAATGCTATACCAGACACAAACAGTCTCCATGGAGGTTGCCCTGTCCTTGAAGGAGAAAAATGGTCAGCAACAAAGTGGATTCACGTAGATTCTTTCAGCAAAAACTTAGGAGACATTGGGAATTGTACTGATCTAAATGAAAGTTGTGAGAGATGGGCTGCCTTAGGGGAATGCACCAAAAACCCAGAATATATGGTTGGATCTCCAGAAATGCCCGGCTACTGTCGGAGGAGTTGCAGGATCTGTTGA

Protein sequence

MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC*

Homology

[Close]

BLAST of CSPI06G05250 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 1.3e-128
Identity = 219/294 (74.49%), Postives = 250/294 (85.03%), Query Frame = 0


Query: 7   LLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSI 66
           LL  F  + S  ++ ST S   S+S  V+PSKVKQ+S KPRAFVYEGFLT+LECDH+VS+
Sbjct: 6   LLISFFAIFSVLLQSST-SLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSL 65

Query: 67  ARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDI 126
           A++ LKRS VADNDSG+SK S VRTSSG FISK KDPIVSGIEDKIS WTFLPKENGEDI
Sbjct: 66  AKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDI 125

Query: 127 QVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRA 186
           QVLRYEHGQKY++H+DYF DKVNI  GGHR+AT+LMYLSNVT+GGETVFP AE PS R  
Sbjct: 126 QVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVL 185

Query: 187 YETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIH 246
            E  EDLS+CAK+G+AVKP+KGDALLFF+L P+AIPD  SLHGGCPV+EGEKWSATKWIH
Sbjct: 186 SENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIH 245

Query: 247 VDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           VDSF + +   GNCTD+NESCERWA LGECTKNPEYMVG+ E+PGYCRRSC+ C
Sbjct: 246 VDSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of CSPI06G05250 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 2.3e-125
Identity = 214/300 (71.33%), Postives = 254/300 (84.67%), Query Frame = 0


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M +   LLF+ ++L    ++ STC    S S+ ++PSKVKQ+S KPRAFVYEGFLTDLEC
Sbjct: 3   MSRLGLLLFVAILLV--LLQSSTC-LISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLEC 62

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHL+S+A+  L+RS VADND+G+S++S VRTSSG FISK KDPIVSGIEDK+S WTFLPK
Sbjct: 63  DHLISLAKENLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPK 122

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGED+QVLRYEHGQKY++H+DYF DKVNIA GGHR+ATVL+YLSNVT+GGETVFP A++
Sbjct: 123 ENGEDLQVLRYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQE 182

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
            S R   E  +DLS+CAKKG+AVKPKKG+ALLFF+L+ +AIPD  SLHGGCPV+EGEKWS
Sbjct: 183 FSRRSLSENKDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWS 242

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSF K L   GNCTD+NESCERWA LGEC KNPEYMVG+PE+PG CRRSC+ C
Sbjct: 243 ATKWIHVDSFDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of CSPI06G05250 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 2.8e-99
Identity = 165/272 (60.66%), Postives = 209/272 (76.84%), Query Frame = 0


Query: 29  SASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGKSKLST 88
           ++S   DP++V Q+SW PR F+YEGFL+D ECDH + +A+ +L++S VADNDSG+S  S 
Sbjct: 46  ASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESE 105

Query: 89  VRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKV 148
           VRTSSGMF+SK +D IVS +E K++AWTFLP+ENGE +Q+L YE+GQKYE H+DYF D+ 
Sbjct: 106 VRTSSGMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQA 165

Query: 149 NIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKGVAVKPKKG 208
           N+  GGHR+ATVLMYLSNV +GGETVFP+ +    +     D+  +ECAK+G AVKP+KG
Sbjct: 166 NLELGGHRIATVLMYLSNVEKGGETVFPMWK---GKATQLKDDSWTECAKQGYAVKPRKG 225

Query: 209 DALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGDIGNCTDLNESCE 268
           DALLFF+L PNA  D+NSLHG CPV+EGEKWSAT+WIHV SF +       C D N SCE
Sbjct: 226 DALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCE 285

Query: 269 RWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           +WA  GEC KNP YMVGS +  GYCR+SC+ C
Sbjct: 286 KWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of CSPI06G05250 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.5e-92
Identity = 162/277 (58.48%), Postives = 207/277 (74.73%), Query Frame = 0


Query: 25  SYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRS-EVADNDSGK 84
           S   S S +VDP+++ Q+SW PRAF+Y+GFL+D ECDHL+ +A+ +L++S  VAD DSG+
Sbjct: 18  SQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGE 77

Query: 85  SKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESHYDY 144
           S+ S VRTSSGMF++K +D IV+ +E K++AWTFLP+ENGE +Q+L YE+GQKY+ H+DY
Sbjct: 78  SEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDY 137

Query: 145 FVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKGVAV 204
           F DK  +  GGHR+ATVLMYLSNVT+GGETVFP       +     D+  S+CAK+G AV
Sbjct: 138 FYDKKALELGGHRIATVLMYLSNVTKGGETVFP---NWKGKTPQLKDDSWSKCAKQGYAV 197

Query: 205 KPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGDIGNCTDL 264
           KP+KGDALLFF+L  N   D NSLHG CPV+EGEKWSAT+WIHV SF K       C D 
Sbjct: 198 KPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGKKK---LVCVDD 257

Query: 265 NESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           +ESC+ WA  GEC KNP YMVGS    G+CR+SC+ C
Sbjct: 258 HESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of CSPI06G05250 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.2e-64
Identity = 114/209 (54.55%), Postives = 156/209 (74.64%), Query Frame = 0


Query: 42  ISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNK 101
           +SW+PRAFVY  FL+  EC++L+S+A+  + +S V D+++GKSK S VRTSSG F+ + +
Sbjct: 79  LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGR 138

Query: 102 DPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVL 161
           D I+  IE +I+ +TF+P ++GE +QVL YE GQKYE HYDYFVD+ N   GG R+AT+L
Sbjct: 139 DKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATML 198

Query: 162 MYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAI 221
           MYLS+V +GGETVFP A    +  +     +LSEC KKG++VKP+ GDALLF+S+ P+A 
Sbjct: 199 MYLSDVEEGGETVFPAAN--MNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDAT 258

Query: 222 PDTNSLHGGCPVLEGEKWSATKWIHVDSF 251
            D  SLHGGCPV+ G KWS+TKW+HV  +
Sbjct: 259 LDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

[Close]

BLAST of CSPI06G05250 vs. ExPASy TrEMBL
Match: A0A0A0KCQ5 (Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_6G067350 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 5.5e-175
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
           PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

[Close]

BLAST of CSPI06G05250 vs. ExPASy TrEMBL
Match: A0A1S3C816 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103497901 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 6.3e-171
Identity = 292/300 (97.33%), Postives = 296/300 (98.67%), Query Frame = 0


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKF NLLF FLIL SSF+RESTCSYAGSASATVDPS+VKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFRNLLFFFLILISSFVRESTCSYAGSASATVDPSRVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVT+GGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
            SHRRAYETDEDLSECAKKG+AVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SSHRRAYETDEDLSECAKKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

[Close]

BLAST of CSPI06G05250 vs. ExPASy TrEMBL
Match: A0A5A7SVW6 (Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001080 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 6.3e-171
Identity = 292/300 (97.33%), Postives = 296/300 (98.67%), Query Frame = 0


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKF NLLF FLIL SSF+RESTCSYAGSASATVDPS+VKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFRNLLFFFLILISSFVRESTCSYAGSASATVDPSRVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVT+GGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
            SHRRAYETDEDLSECAKKG+AVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SSHRRAYETDEDLSECAKKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

[Close]

BLAST of CSPI06G05250 vs. ExPASy TrEMBL
Match: A0A6J1H545 (Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111460227 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 5.0e-160
Identity = 275/300 (91.67%), Postives = 290/300 (96.67%), Query Frame = 0


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF NLLF+FLIL SS +RES+CSYAGSA++TVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRNLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG SKLSTVRTSSGMFISK+KDPIVSGIEDKI+AWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIA GGHRLATVLMYLSNVT+GGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
              RRA ETDEDLSECA++G+AVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLSECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLG+IG+C+DLNESCERWAALGECTKNPEYMVGSPE+PGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCSDLNESCERWAALGECTKNPEYMVGSPELPGYCRRSCRIC 300

[Close]

BLAST of CSPI06G05250 vs. ExPASy TrEMBL
Match: A0A6J1L4G1 (Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499059 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.2e-158
Identity = 274/300 (91.33%), Postives = 288/300 (96.00%), Query Frame = 0


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF  LLF+FLIL SS +RES+CSYAGSA++TVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRYLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG SKLSTVRTSSGMFISK+KDPIVSGIEDKI+AWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIA GGHRLATVLMYLSNVT+GGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
              RRA ETDEDLSECA++G+AVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLSECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLG+IG+CTDLNESCERWAALGECTKNPEYMVGS E+PGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCTDLNESCERWAALGECTKNPEYMVGSAELPGYCRRSCRIC 300

[Close]

BLAST of CSPI06G05250 vs. NCBI nr
Match: XP_004144967.1 (probable prolyl 4-hydroxylase 4 [Cucumis sativus] >XP_011656650.1 probable prolyl 4-hydroxylase 4 [Cucumis sativus] >KGN46177.1 hypothetical protein Csa_004844 [Cucumis sativus])

HSP 1 Score: 623.2 bits (1606), Expect = 1.1e-174
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
           PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

[Close]

BLAST of CSPI06G05250 vs. NCBI nr
Match: XP_008458517.1 (PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis melo] >XP_008458518.1 PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis melo] >KAA0033447.1 putative prolyl 4-hydroxylase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 609.8 bits (1571), Expect = 1.3e-170
Identity = 292/300 (97.33%), Postives = 296/300 (98.67%), Query Frame = 0


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKF NLLF FLIL SSF+RESTCSYAGSASATVDPS+VKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFRNLLFFFLILISSFVRESTCSYAGSASATVDPSRVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVT+GGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
            SHRRAYETDEDLSECAKKG+AVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SSHRRAYETDEDLSECAKKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

[Close]

BLAST of CSPI06G05250 vs. NCBI nr
Match: XP_038874583.1 (probable prolyl 4-hydroxylase 4 [Benincasa hispida])

HSP 1 Score: 599.0 bits (1543), Expect = 2.3e-167
Identity = 286/300 (95.33%), Postives = 295/300 (98.33%), Query Frame = 0


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKF NLLFIFLIL S  +RESTCSYAGSAS+TVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFRNLLFIFLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISK+KDPIVSGIEDKISAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKSKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHG+KYESHYDYFVDKVNIAWGGHRLATVLMYLSNVT+GGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGEKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
            +HRRAYETDEDLSECA+KG+AVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SAHRRAYETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLG+IGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

[Close]

BLAST of CSPI06G05250 vs. NCBI nr
Match: KAG6575033.1 (putative prolyl 4-hydroxylase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 573.9 bits (1478), Expect = 7.9e-160
Identity = 275/300 (91.67%), Postives = 290/300 (96.67%), Query Frame = 0


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF NLLF+FLIL SS +RES+CSYAGSA++TVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRNLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG SKLSTVRTSSGMFISK+KDPIVSGIEDKI+AWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIA GGHRLATVLMYLSNVT+GGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
              RRA ETDEDL+ECA++G+AVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLTECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLG+IG+CTDLNESCERWAALGECTKNPEYMVGSPE+PGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRRSCRIC 300

[Close]

BLAST of CSPI06G05250 vs. NCBI nr
Match: XP_022959148.1 (probable prolyl 4-hydroxylase 4 [Cucurbita moschata] >XP_022959149.1 probable prolyl 4-hydroxylase 4 [Cucurbita moschata])

HSP 1 Score: 573.5 bits (1477), Expect = 1.0e-159
Identity = 275/300 (91.67%), Postives = 290/300 (96.67%), Query Frame = 0


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF NLLF+FLIL SS +RES+CSYAGSA++TVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRNLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG SKLSTVRTSSGMFISK+KDPIVSGIEDKI+AWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIA GGHRLATVLMYLSNVT+GGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
              RRA ETDEDLSECA++G+AVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLSECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLG+IG+C+DLNESCERWAALGECTKNPEYMVGSPE+PGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCSDLNESCERWAALGECTKNPEYMVGSPELPGYCRRSCRIC 300

[Close]

BLAST of CSPI06G05250 vs. TAIR 10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 460.7 bits (1184), Expect = 9.1e-130
Identity = 219/294 (74.49%), Postives = 250/294 (85.03%), Query Frame = 0


Query: 7   LLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSI 66
           LL  F  + S  ++ ST S   S+S  V+PSKVKQ+S KPRAFVYEGFLT+LECDH+VS+
Sbjct: 6   LLISFFAIFSVLLQSST-SLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSL 65

Query: 67  ARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDI 126
           A++ LKRS VADNDSG+SK S VRTSSG FISK KDPIVSGIEDKIS WTFLPKENGEDI
Sbjct: 66  AKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDI 125

Query: 127 QVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRA 186
           QVLRYEHGQKY++H+DYF DKVNI  GGHR+AT+LMYLSNVT+GGETVFP AE PS R  
Sbjct: 126 QVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVL 185

Query: 187 YETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIH 246
            E  EDLS+CAK+G+AVKP+KGDALLFF+L P+AIPD  SLHGGCPV+EGEKWSATKWIH
Sbjct: 186 SENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIH 245

Query: 247 VDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           VDSF + +   GNCTD+NESCERWA LGECTKNPEYMVG+ E+PGYCRRSC+ C
Sbjct: 246 VDSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of CSPI06G05250 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 449.9 bits (1156), Expect = 1.6e-126
Identity = 214/300 (71.33%), Postives = 254/300 (84.67%), Query Frame = 0


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M +   LLF+ ++L    ++ STC    S S+ ++PSKVKQ+S KPRAFVYEGFLTDLEC
Sbjct: 3   MSRLGLLLFVAILLV--LLQSSTC-LISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLEC 62

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHL+S+A+  L+RS VADND+G+S++S VRTSSG FISK KDPIVSGIEDK+S WTFLPK
Sbjct: 63  DHLISLAKENLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPK 122

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGED+QVLRYEHGQKY++H+DYF DKVNIA GGHR+ATVL+YLSNVT+GGETVFP A++
Sbjct: 123 ENGEDLQVLRYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQE 182

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
            S R   E  +DLS+CAKKG+AVKPKKG+ALLFF+L+ +AIPD  SLHGGCPV+EGEKWS
Sbjct: 183 FSRRSLSENKDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWS 242

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSF K L   GNCTD+NESCERWA LGEC KNPEYMVG+PE+PG CRRSC+ C
Sbjct: 243 ATKWIHVDSFDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of CSPI06G05250 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 363.2 bits (931), Expect = 2.0e-100
Identity = 165/272 (60.66%), Postives = 209/272 (76.84%), Query Frame = 0


Query: 29  SASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGKSKLST 88
           ++S   DP++V Q+SW PR F+YEGFL+D ECDH + +A+ +L++S VADNDSG+S  S 
Sbjct: 46  ASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESE 105

Query: 89  VRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKV 148
           VRTSSGMF+SK +D IVS +E K++AWTFLP+ENGE +Q+L YE+GQKYE H+DYF D+ 
Sbjct: 106 VRTSSGMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQA 165

Query: 149 NIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKGVAVKPKKG 208
           N+  GGHR+ATVLMYLSNV +GGETVFP+ +    +     D+  +ECAK+G AVKP+KG
Sbjct: 166 NLELGGHRIATVLMYLSNVEKGGETVFPMWK---GKATQLKDDSWTECAKQGYAVKPRKG 225

Query: 209 DALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGDIGNCTDLNESCE 268
           DALLFF+L PNA  D+NSLHG CPV+EGEKWSAT+WIHV SF +       C D N SCE
Sbjct: 226 DALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCE 285

Query: 269 RWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           +WA  GEC KNP YMVGS +  GYCR+SC+ C
Sbjct: 286 KWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of CSPI06G05250 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 340.9 bits (873), Expect = 1.1e-93
Identity = 159/280 (56.79%), Postives = 204/280 (72.86%), Query Frame = 0


Query: 29  SASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGKS---- 88
           ++S   DP++V Q+SW PR F+YEGFL+D ECDH + +A+ +L++S VADNDSG+S    
Sbjct: 46  ASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESE 105

Query: 89  -KLSTVRTSSGMFISKNK---DPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESH 148
             +S VR SS    + +    D IVS +E K++AWTFLP+ENGE +Q+L YE+GQKYE H
Sbjct: 106 DSVSVVRQSSSFIANMDSLEIDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPH 165

Query: 149 YDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKG 208
           +DYF D+ N+  GGHR+ATVLMYLSNV +GGETVFP+ +    +     D+  +ECAK+G
Sbjct: 166 FDYFHDQANLELGGHRIATVLMYLSNVEKGGETVFPMWK---GKATQLKDDSWTECAKQG 225

Query: 209 VAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGDIGNC 268
            AVKP+KGDALLFF+L PNA  D+NSLHG CPV+EGEKWSAT+WIHV SF +       C
Sbjct: 226 YAVKPRKGDALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGC 285

Query: 269 TDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
            D N SCE+WA  GEC KNP YMVGS +  GYCR+SC+ C
Sbjct: 286 MDENVSCEKWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 322

[Close]

BLAST of CSPI06G05250 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 340.1 bits (871), Expect = 1.8e-93
Identity = 162/277 (58.48%), Postives = 207/277 (74.73%), Query Frame = 0


Query: 25  SYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRS-EVADNDSGK 84
           S   S S +VDP+++ Q+SW PRAF+Y+GFL+D ECDHL+ +A+ +L++S  VAD DSG+
Sbjct: 18  SQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGE 77

Query: 85  SKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESHYDY 144
           S+ S VRTSSGMF++K +D IV+ +E K++AWTFLP+ENGE +Q+L YE+GQKY+ H+DY
Sbjct: 78  SEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDY 137

Query: 145 FVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKGVAV 204
           F DK  +  GGHR+ATVLMYLSNVT+GGETVFP       +     D+  S+CAK+G AV
Sbjct: 138 FYDKKALELGGHRIATVLMYLSNVTKGGETVFP---NWKGKTPQLKDDSWSKCAKQGYAV 197

Query: 205 KPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGDIGNCTDL 264
           KP+KGDALLFF+L  N   D NSLHG CPV+EGEKWSAT+WIHV SF K       C D 
Sbjct: 198 KPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGKKK---LVCVDD 257

Query: 265 NESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           +ESC+ WA  GEC KNP YMVGS    G+CR+SC+ C
Sbjct: 258 HESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

The following BLAST results are available for this feature:

BLAST of CSPI06G05250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LAN3	1.3e-128	74.49	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]
F4JAU3	2.3e-125	71.33	Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1	[more]
Q8L970	2.8e-99	60.66	Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=...	[more]
F4J0A8	2.5e-92	58.48	Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=...	[more]
Q9LN20	2.2e-64	54.55	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]

BLAST of CSPI06G05250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KCQ5	5.5e-175	100.00	Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_6G067350 PE=...	[more]
A0A1S3C816	6.3e-171	97.33	Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103497901 PE=3 S...	[more]
A0A5A7SVW6	6.3e-171	97.33	Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A6J1H545	5.0e-160	91.67	Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111460227 ...	[more]
A0A6J1L4G1	1.2e-158	91.33	Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499059 PE...	[more]

BLAST of CSPI06G05250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144967.1	1.1e-174	100.00	probable prolyl 4-hydroxylase 4 [Cucumis sativus] >XP_011656650.1 probable proly...	[more]
XP_008458517.1	1.3e-170	97.33	PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis melo] >XP_008458518.1 PREDIC...	[more]
XP_038874583.1	2.3e-167	95.33	probable prolyl 4-hydroxylase 4 [Benincasa hispida]	[more]
KAG6575033.1	7.9e-160	91.67	putative prolyl 4-hydroxylase 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022959148.1	1.0e-159	91.67	probable prolyl 4-hydroxylase 4 [Cucurbita moschata] >XP_022959149.1 probable pr...	[more]

BLAST of CSPI06G05250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G18900.1	9.1e-130	74.49	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G06300.1	1.6e-126	71.33	P4H isoform 2	[more]
AT3G28480.1	2.0e-100	60.66	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28480.2	1.1e-93	56.79	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28490.1	1.8e-93	58.48	Oxoglutarate/iron-dependent oxygenase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 46..246 e-value: 1.9E-62 score: 223.5
IPR003582	ShKT domain	SMART	SM00254	ShkT_1	coord: 259..300 e-value: 0.0067 score: 25.6
IPR003582	ShKT domain	PFAM	PF01549	ShK	coord: 259..300 e-value: 4.8E-6 score: 27.0
IPR003582	ShKT domain	PROSITE	PS51670	SHKT	coord: 260..300 score: 8.880531
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 38..247 e-value: 5.2E-75 score: 253.7
None	No IPR available	PANTHER	PTHR10869:SF175	PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-LIKE PROTEIN	coord: 8..300
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 126..246 e-value: 4.3E-20 score: 72.4
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 8..300
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 122..247 score: 12.514795

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G05250.1	CSPI06G05250.1	mRNA
CSPI06G05250.2	CSPI06G05250.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component	GO:0005789	endoplasmic reticulum membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen