CSPI06G04020 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G04020
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	ATPase inhibitor, mitochondrial-like
Location	Chr6: 3736501 .. 3738344 (+)
RNA-Seq Expression	CSPI06G04020
Synteny	CSPI06G04020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAGGCTCAATTAGTGTTAGGGCTATAAAAATGAACCGACGGAACCATGCTGTCACTCTCTAGAACATTTCGATTTCCATAAGCTCAAATGGCCATGCGATCGGTGCTCACTCGATCATCGTTGACTACTTCGACGGCGCGTGCCGGTGGATTCCGCTTCCTCTCCGACGGCAAAGGTCGAGTGCTTAGTGAGGAGGAGCGTGCTGCCGAGAACGTCTACATCCAGGTTGAATTATTAGTTTCGAATGTTCGAGAAAAGTTTGTAGAAACCAGTAGAACGCCGAGTGTCGACCGGTTTTTGTAATTTTCAGTTGTTACAAACTCAGATGGAATGGTTTAGACTCTAGAGCGAGAGCGAAGTTACTGTTTCGATACTTTCTTTTTTTGTGCTCGGAATTTGCTTTCTCTGATTTATCGCGGAGTATGGTCTTCAATCTGTTTGACCGAATACACACTTGTTCGAAACTCTAATAAAAATGAACTGGAGTGGGAGCATGTTTACTGTTTATGTCAGATGGTATTTGTGAGGAATTTGTGATCTCTGATTTACTGCAGTAGGAGTTGGCTTGGCGAATTTCCATTTTCTCGAAACTTTAATAGAAATGCACAAGACTGTAAATGAAGTTATGGAATCTATGTTCTCCGATTTTGAGTTATTGTTTTTCTAATGTTTGTAGAAAATGGAAAGAGAAAGGTTGGAGAAGCTGAGGCTTAAGGCAGAGAAAGAAAAGGCTCAGAAGGAGAAAGAACAGTCTGAGAAGGTATTCGTCACTCTTATAATTTTGGTTGAAATTGTCGAATATTGTTTATTAGTTGATTTGTGATATGTATGACAGTGAAAAACTGTGTTTTGTGATTATGCAACATTTCTTTTCATCATGCTTTATGGCTTTGTGGGATTTTGGATCCATCGTCATCCTTTGTTTGTTCCCAACTCTTTGCTTGAGTAGCTTGCACTGACTATTTGTTCGATCAGTATTTGATTTCTTGTATCTATATGGAAATTGAGCCCAAGCTTCTGATAAATAGATATCGATTGCAAGATATTATGGTCAGCTTTTGCTTGTGTCCAATCCAATTATGGTTCATTAGGAACTACGATTTTCATAGCGGCTGAAATTTAATGGGATTTAATCCTTGAATCTTCGTTTCTTGTTTCCTGCAGAAATTTGTTACCACTATTAAAGTATTCTGTAAATAAAGCCCGAATCTAGAATGCTGTTATGGCAGTATGCCGAAGCACCTTTTTGTTCCTTATCTCTTTCTTTCGGTGCTTCGTGTCTCTAGTCATCAGACTGATCGGTTTATACTTGAAATATGACAAAGTTTTGGTACTACTCTTGTGATATGACAAACTTTCTCACTAATTTTTGTACTTTTACCATTTCCCAAACAAATTTACAAGTTGAGTTATTAAATTCCACTTCAGGAGTAGCATATCTATCTTCTAAGGAGTGTGAGGCCTTTTGGTTTTTCTTTTTTCAATGTGGGTTCTCAACAGATCTCTCAAGATGATGTCCCTTTTGTGTTTACCATTCTTTTTTATTGGACCTAATACTTGCCCATATTAGATAATATGGGTTGACATGAGATTCTCCTCCGCTTGATAATGATGATGTCTCACTATATCTATAAGGAAAAAGTTGTAATGTCATAATTTAAATGTTTCAATATTAACTTAACTCTCGTTTCTCATATTGTAAACTTTGTTTACCCTTTTTCTCATCATTGTTTCATATCTGCAGAAACATGAAGGGACCCAGTAGAAAAAGACCGTGGGCACATCTGCTTTGAAGTTTGATCTTGATCGCATGGAATAAAATGTGAGATTTCACCCCCCCCC

mRNA sequence

GAAAGGCTCAATTAGTGTTAGGGCTATAAAAATGAACCGACGGAACCATGCTGTCACTCTCTAGAACATTTCGATTTCCATAAGCTCAAATGGCCATGCGATCGGTGCTCACTCGATCATCGTTGACTACTTCGACGGCGCGTGCCGGTGGATTCCGCTTCCTCTCCGACGGCAAAGGTCGAGTGCTTAGTGAGGAGGAGCGTGCTGCCGAGAACGTCTACATCCAGAAAATGGAAAGAGAAAGGTTGGAGAAGCTGAGGCTTAAGGCAGAGAAAGAAAAGGCTCAGAAGGAGAAAGAACAGTCTGAGAAGAAACATGAAGGGACCCAGTAGAAAAAGACCGTGGGCACATCTGCTTTGAAGTTTGATCTTGATCGCATGGAATAAAATGTGAGATTTCACCCCCCCCC

Coding sequence (CDS)

ATGGCCATGCGATCGGTGCTCACTCGATCATCGTTGACTACTTCGACGGCGCGTGCCGGTGGATTCCGCTTCCTCTCCGACGGCAAAGGTCGAGTGCTTAGTGAGGAGGAGCGTGCTGCCGAGAACGTCTACATCCAGAAAATGGAAAGAGAAAGGTTGGAGAAGCTGAGGCTTAAGGCAGAGAAAGAAAAGGCTCAGAAGGAGAAAGAACAGTCTGAGAAGAAACATGAAGGGACCCAGTAG

Protein sequence

MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKAEKEKAQKEKEQSEKKHEGTQ*

Homology

[Close]

BLAST of CSPI06G04020 vs. ExPASy TrEMBL
Match: A0A0A0K8J7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G046230 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.9e-29
Identity = 80/80 (100.00%), Postives = 80/80 (100.00%), Query Frame = 0


Query: 1  MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKA 60
          MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKA
Sbjct: 1  MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKA 60

Query: 61 EKEKAQKEKEQSEKKHEGTQ 81
          EKEKAQKEKEQSEKKHEGTQ
Sbjct: 61 EKEKAQKEKEQSEKKHEGTQ 80

[Close]

BLAST of CSPI06G04020 vs. ExPASy TrEMBL
Match: A0A1S3C383 (uncharacterized protein LOC103496516 OS=Cucumis melo OX=3656 GN=LOC103496516 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 9.7e-26
Identity = 76/83 (91.57%), Postives = 78/83 (93.98%), Query Frame = 0


Query: 1  MAMRSVLTRSSLTTSTAR---AGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60
          MAMRSVL+RSSLT  TAR   AGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR
Sbjct: 1  MAMRSVLSRSSLTPCTARAAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60

Query: 61 LKAEKEKAQKEKEQSEKKHEGTQ 81
          LKAEKEKA+KEKEQSEKKHEGTQ
Sbjct: 61 LKAEKEKAEKEKEQSEKKHEGTQ 83

[Close]

BLAST of CSPI06G04020 vs. ExPASy TrEMBL
Match: A0A5D3CIM5 (Uncharacterized transmembrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold304G001000 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.2e-22
Identity = 70/77 (90.91%), Postives = 72/77 (93.51%), Query Frame = 0


Query: 1  MAMRSVLTRSSLTTSTAR---AGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60
          MAMRSVL+RSSLT  TAR   AGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR
Sbjct: 1  MAMRSVLSRSSLTPCTARAAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60

Query: 61 LKAEKEKAQKEKEQSEK 75
          LKAEKEKA+KEKEQSEK
Sbjct: 61 LKAEKEKAEKEKEQSEK 77

[Close]

BLAST of CSPI06G04020 vs. ExPASy TrEMBL
Match: A0A6J1JS80 (uncharacterized protein LOC111487345 OS=Cucurbita maxima OX=3661 GN=LOC111487345 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.8e-22
Identity = 70/83 (84.34%), Postives = 74/83 (89.16%), Query Frame = 0


Query: 1  MAMRSVLTRSSLTTSTARA---GGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60
          MAMRSV  RSSLT ST RA   GGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEK R
Sbjct: 1  MAMRSVFGRSSLTPSTVRAAGVGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKQR 60

Query: 61 LKAEKEKAQKEKEQSEKKHEGTQ 81
          LKAEKE+A+K++EQSEKK EGTQ
Sbjct: 61 LKAEKERAEKKEEQSEKKREGTQ 83

[Close]

BLAST of CSPI06G04020 vs. ExPASy TrEMBL
Match: A0A6J1JUA9 (beta-mannosyltransferase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111488968 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.9e-21
Identity = 69/83 (83.13%), Postives = 73/83 (87.95%), Query Frame = 0


Query: 1  MAMRSVLTRSSLTTSTARA---GGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60
          MAMRSVL+R S T  T RA   GGFR+ SDGKGRVLSEEERAAENVYIQKMERE+LEKLR
Sbjct: 1  MAMRSVLSRISPTPLTMRASGPGGFRYFSDGKGRVLSEEERAAENVYIQKMEREKLEKLR 60

Query: 61 LKAEKEKAQKEKEQSEKKHEGTQ 81
          LKAEKEKA+KEKEQSEKK EGTQ
Sbjct: 61 LKAEKEKAEKEKEQSEKKREGTQ 83

[Close]

BLAST of CSPI06G04020 vs. NCBI nr
Match: XP_004140909.1 (uncharacterized protein At2g27730, mitochondrial [Cucumis sativus] >KGN46045.1 hypothetical protein Csa_005746 [Cucumis sativus])

HSP 1 Score: 137.9 bits (346), Expect = 3.9e-29
Identity = 80/80 (100.00%), Postives = 80/80 (100.00%), Query Frame = 0


Query: 1  MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKA 60
          MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKA
Sbjct: 1  MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKA 60

Query: 61 EKEKAQKEKEQSEKKHEGTQ 81
          EKEKAQKEKEQSEKKHEGTQ
Sbjct: 61 EKEKAQKEKEQSEKKHEGTQ 80

[Close]

BLAST of CSPI06G04020 vs. NCBI nr
Match: XP_008456607.1 (PREDICTED: uncharacterized protein LOC103496516 [Cucumis melo])

HSP 1 Score: 125.6 bits (314), Expect = 2.0e-25
Identity = 76/83 (91.57%), Postives = 78/83 (93.98%), Query Frame = 0


Query: 1  MAMRSVLTRSSLTTSTAR---AGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60
          MAMRSVL+RSSLT  TAR   AGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR
Sbjct: 1  MAMRSVLSRSSLTPCTARAAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60

Query: 61 LKAEKEKAQKEKEQSEKKHEGTQ 81
          LKAEKEKA+KEKEQSEKKHEGTQ
Sbjct: 61 LKAEKEKAEKEKEQSEKKHEGTQ 83

[Close]

BLAST of CSPI06G04020 vs. NCBI nr
Match: XP_038884176.1 (uncharacterized protein LOC120075086 [Benincasa hispida])

HSP 1 Score: 120.6 bits (301), Expect = 6.5e-24
Identity = 74/83 (89.16%), Postives = 76/83 (91.57%), Query Frame = 0


Query: 1  MAMRSVLTRSSLTTSTAR---AGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60
          MAMRSVL RSSLT  TAR   AGGFRFLSDGKGR+LSEEERAAENVYIQKMERERLEKLR
Sbjct: 1  MAMRSVLGRSSLTPLTARAAGAGGFRFLSDGKGRILSEEERAAENVYIQKMERERLEKLR 60

Query: 61 LKAEKEKAQKEKEQSEKKHEGTQ 81
          LKAEKEKA+KEKEQSEKK EGTQ
Sbjct: 61 LKAEKEKAEKEKEQSEKKREGTQ 83

[Close]

BLAST of CSPI06G04020 vs. NCBI nr
Match: TYK11773.1 (putative uncharacterized transmembrane protein [Cucumis melo var. makuwa])

HSP 1 Score: 114.4 bits (285), Expect = 4.6e-22
Identity = 70/77 (90.91%), Postives = 72/77 (93.51%), Query Frame = 0


Query: 1  MAMRSVLTRSSLTTSTAR---AGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60
          MAMRSVL+RSSLT  TAR   AGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR
Sbjct: 1  MAMRSVLSRSSLTPCTARAAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60

Query: 61 LKAEKEKAQKEKEQSEK 75
          LKAEKEKA+KEKEQSEK
Sbjct: 61 LKAEKEKAEKEKEQSEK 77

[Close]

BLAST of CSPI06G04020 vs. NCBI nr
Match: XP_022990504.1 (uncharacterized protein LOC111487345 [Cucurbita maxima] >XP_023523530.1 uncharacterized protein LOC111787725 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 113.6 bits (283), Expect = 7.9e-22
Identity = 70/83 (84.34%), Postives = 74/83 (89.16%), Query Frame = 0


Query: 1  MAMRSVLTRSSLTTSTARA---GGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60
          MAMRSV  RSSLT ST RA   GGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEK R
Sbjct: 1  MAMRSVFGRSSLTPSTVRAAGVGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKQR 60

Query: 61 LKAEKEKAQKEKEQSEKKHEGTQ 81
          LKAEKE+A+K++EQSEKK EGTQ
Sbjct: 61 LKAEKERAEKKEEQSEKKREGTQ 83

[Close]

BLAST of CSPI06G04020 vs. TAIR 10
Match: AT5G04750.1 (F1F0-ATPase inhibitor protein, putative )

HSP 1 Score: 52.8 bits (125), Expect = 1.5e-07
Identity = 37/68 (54.41%), Postives = 47/68 (69.12%), Query Frame = 0


Query: 10 SSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKAEKEKAQKEK 69
          +S +  + R    R+ SD KGRVLSEEERA E++YIQKMERERLEK + K E++K   EK
Sbjct: 24 ASRSVVSTRGPAIRYFSDDKGRVLSEEERAKESMYIQKMERERLEK-KKKLEQDKLDGEK 83

Query: 70 EQSEKKHE 78
            + KK E
Sbjct: 84 GSANKKPE 90

[Close]

BLAST of CSPI06G04020 vs. TAIR 10
Match: AT2G27730.1 (copper ion binding )

HSP 1 Score: 40.4 bits (93), Expect = 7.9e-04
Identity = 28/53 (52.83%), Postives = 37/53 (69.81%), Query Frame = 0


Query: 13 TTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKAEKEKA 66
          T +  R    RF S   G+VLSEEERAAENV+I+KME+E+L+KL  +   E+A
Sbjct: 3  TRNALRIVSRRFSS---GKVLSEEERAAENVFIKKMEQEKLQKLARQGPGEQA 52

The following BLAST results are available for this feature:

BLAST of CSPI06G04020 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI06G04020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K8J7	1.9e-29	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G046230 PE=3 SV=1	[more]
A0A1S3C383	9.7e-26	91.57	uncharacterized protein LOC103496516 OS=Cucumis melo OX=3656 GN=LOC103496516 PE=...	[more]
A0A5D3CIM5	2.2e-22	90.91	Uncharacterized transmembrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A6J1JS80	3.8e-22	84.34	uncharacterized protein LOC111487345 OS=Cucurbita maxima OX=3661 GN=LOC111487345...	[more]
A0A6J1JUA9	1.9e-21	83.13	beta-mannosyltransferase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111488968 PE=3...	[more]

BLAST of CSPI06G04020 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140909.1	3.9e-29	100.00	uncharacterized protein At2g27730, mitochondrial [Cucumis sativus] >KGN46045.1 h...	[more]
XP_008456607.1	2.0e-25	91.57	PREDICTED: uncharacterized protein LOC103496516 [Cucumis melo]	[more]
XP_038884176.1	6.5e-24	89.16	uncharacterized protein LOC120075086 [Benincasa hispida]	[more]
TYK11773.1	4.6e-22	90.91	putative uncharacterized transmembrane protein [Cucumis melo var. makuwa]	[more]
XP_022990504.1	7.9e-22	84.34	uncharacterized protein LOC111487345 [Cucurbita maxima] >XP_023523530.1 uncharac...	[more]

BLAST of CSPI06G04020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G04750.1	1.5e-07	54.41	F1F0-ATPase inhibitor protein, putative	[more]
AT2G27730.1	7.9e-04	52.83	copper ion binding	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 45..80
None	No IPR available	GENE3D	1.20.5.500	Single helix bin	coord: 14..77 e-value: 1.4E-5 score: 26.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 58..80
None	No IPR available	PANTHER	PTHR33878:SF4	OS02G0533800 PROTEIN	coord: 1..77
None	No IPR available	PANTHER	PTHR33878	OS08G0559000 PROTEIN	coord: 1..77
IPR007648	Mitochondrial ATPase inhibitor	PFAM	PF04568	IATP	coord: 27..74 e-value: 1.7E-5 score: 25.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G04020.1	CSPI06G04020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032780	negative regulation of ATP-dependent activity
cellular_component	GO:0005747	mitochondrial respiratory chain complex I
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0042030	ATPase inhibitor activity
molecular_function	GO:0016757	glycosyltransferase activity