CSPI06G03660 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G03660
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Non-specific serine/threonine protein kinase
Location	Chr6: 3373136 .. 3377006 (+)
RNA-Seq Expression	CSPI06G03660
Synteny	CSPI06G03660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTGAGCCTCCCTCATACGTGCTTCCGATGTCAATTGGTGTCGGCTGGTTACATCTGCTTGTAAAGAAGGTTCTAACCTGGTTATAAACACATCCATGAGCACACTTTCAGCCATGTCCGTAAGAGGGCTGAGTAATTTAAGAGTTTCTTAACATAATCACTGTAGGATCCATCTTGGGTAATCCTAATTAGTTCTGCTCCCAAGCTCCCTTGTTCAATGTCCCTAAAATGTTGGAAAATCCTCTTTCAAATCCGACCATGATTCAACTTTCCTTCGATTGTGGCTCTATCTATACCAGTCCACTTCATCTTGGCCAAAACTCACCACAGCAACTTTGACCTTTTCAGCTTCTGGAAGATCATTAATCTAAAAAAAAATGTCCTGCACAGTAAACCCATGATTCGGGGTTCTTTACAAGGAACAGGGGCATTTCCAGCTTTTTATACTTCTATCCACAGTGACTGTCGTGTTGTCCAAACGAGTTTACAAGTCACCCATTTTCCCCTTTTGTTTCATCACAGATCCATCGGACATTCCAGATTCATATCTCCTTCGATATAATCTATTTTCTTTCATGTTATCAGCCATACGGTCAACTGCCTTCCTCAACTCGAGCATCATTTCCTTCAAACCCAGAACTTTTTCAGTACCTTCCAAGCACTCTTCCATTTGTTTATTCACCATTAATCTTGTCACAACCCCTAGATTTATGGCTCTGATACCAATATGTTAGGATTCCTCTCTTAAAAGACAATAATAATCCCAACAAAATGATAGAAACAGAGGCAGCACTCTGTCATTTACTTATATTCAATAAACAGAAAGTTCAACAAGGAAAATATCAAGAGAAAAGTAAATAAACAGCATGAAATACACACCAGACCAGCAAAGAAAGGTACAGAAATAGCCCATCCTTAATATGGCTGACGGCTCCTCCCTAAAATTATTCTATTTATACTCATCATTTGTGGGCCCTGCCAGCATCATTCTTTTCTTTAATACTCTCTCCCAGCTCTGTCCCCTATACAATTACCTTCTTACTTTTTCTTTTCTACTGATGAATAATAGGAGGCCTTGCAATGTGCTGCAGTGAGAACATCACCCTATAGATAGGAAGGAAGGAAATGGACAACATAAGGGAACGAAAAACTTCTAAAAGGTGACGGTTCTTTTGCTTATAAACCCCATTTTGATGGAGGGTGTAAGCACAGACACTTAGGTGAATAATTCCTTTAGATGACAAATATTAAACCACCAATTCATCCAAAAGCTTAAGCTAGTGGTTGAAGGCAAATTTAATTATATATCACCAACAACAAAAACTCATTAAGGGTGTGGTTTTGGAACTCACGACCATTATCACATCGTAGGTTTCAATTTTTGTATTGAATTACATTTCTACGATGTGATAGAAGTTCCAAAATGTGGAAGTTCCAGATTTGTTGAAGATAAGGAAAACCCAAGTAAGATGTTAAGGTCGTCAATAAAGGTCACAAACCACCGTTTTCCAGGGGAGGTACCGACCTTGGATGGTCCCCAAACATCACTATGAACAAGAGTGAAAAGTTGGATTGGCTTGTATGGTGGTGAGAGAAAAGAGACTCGATGCTATGTAGCCCCAATGCACACATCACAAGTTAAATAAGAAACATCAACTTTAGAAAAAAGATGAGAAAGTAAGTCCTTCATATATTGAAAGTTGGGGTGGCCTAAATGAAAATATCACAACATACAGTCTTGAAAATAAAAGACTAGTCCTAGAAATACTACTAGAGGAGGCATCATCATCAAGCAGGTAGAGTCCCCTATCGTGTCAGCCAGTGCCAATCATCTTCCCGAAGCTCAAGTCCTGAAAAGAAACATAATCAAGTAAGAATTTTGTTTTGTAGTTGAATCCACTTGTAATCTTGTTTATTGATTGAAGATTATAGGAAATTCTGCACATGCAACACATTGTGTAAGGTTAATCCTTCAAAAGGAGAAACCTTTTCCCTTCCCGGCAATGAGAGCCAAGGAGCCATTTGCAATTCTTGTTTTCTCACTTTTAGCACACAGAATGTAGGGCATAGAATGCTCACAAAAACTTGTCAAATGATTTGTGGCGCCAGAGTCTAAGAAACCCATCAACACTAATAATAAGACTGAAGGACTGAGATATACCTGTATGGTAAATAGCTCCAAGTGTAGTCGGGCTGGGATCCCTAAGTGGCTGAGAAGGGTCGGCACACTCATTCACATAAGCCCATCCTGGGTTTTGTTTGTCGTTGGAAGTGCGTCTCTTCCTTTGGCGAACACCATGTAACTTCCTGATTCAAGTCGAAGAAATCTCGCAGCTTTGTTACCAATTAATGTAAAGCAAAGAAGAATCTTATTCAAAATGTCAATTAATTCTTACAAAACAGAACAGCCGAGAATATATATGTCTACTCGATAGCCAACCTACAACCTGCTGGACAGGTTGTAAACAAACCAAAGACGTGTGTTAACCCTTACAAAACAGACATTTAATAAGTTGGGATTAAGTAAGCAACTACAATAACAGAACATACAAAAACTTTCAGCATACATCAACTACATCATCATTTTGTTCCGTTGTGAACTTTTCAGAAATGAGGGGAGCACTAGTGTCTTGATTTGTTCACGAGGAGGTGGGCTATTGTTGGAACGCATGGAACTTGTTGTGGAGCTATGGGAGGAGAAGATTAAGGTTGGTATTAGTATATCGGATAATCTACTGTCTATTTAATTGTTCATCTTGAGAAGTGTCTTTTTGTTTGATCAGGCTCAGCTTGTCCCTACACCTGATCCCAGCCTAACGGAGCAATATGAATATGCTAACGAGCATGACATAAAATGTCTTGTTATTATTACGGACTCTGGTGTATCTAACACAGGTTCAGTAAAGGTAAAACTTCTAATCTAAATTTCTACTTCTTGGAGCAGCATTTAGGAGTTAAAGCTAAAATGTTGGAAGTGAAGGCATATTAGTCAAACTAGTTCTTCATTCCGCCATTGTTTATAATTCGAAAGTGTACCAAATCATGGCTTATGTCTTGGTTTCCTTGTTTGGTTCAGTTTGGTTCTTTATTTTTAGCAAGGTCTTTACGTTGTTAACCTTTTGTCGTAATCATCAGTACTTTGAACATTACTCTCTTCATTTATTTAATGAAATTTCTGTATCCTTTTTAAAAAGAAAAAATAAATAAATAATCTTTATTGTATTGCAAATAGGATGGAAGAGTTTTGGACGTACTACCCTCTGGAGGGCTTGACTATTTATAACGGTCCAAGTTAACCATTTTAAACGACTCAATTACTTGTGCATGTAAATAAGTTTTCCTAAATCAGTCGTCGTCTGTATGTTGTTGCCTGCTCTCTTACGTTGCACAAAAGCATGGTTCTTATAGATCACATTACATATCCAGGTTCGACACCTAGAACTCAAAAAGGAGAAGAAAGTTGAACGAACATACATTGTCAAGTTTCTCCAGGAGGCAATGGCCAATCAGTTTAAAAATCCTCTCATTTGGAATTAGAACTGTTCTTCATTGCATTACTCCGGCTTCTACTCATCCGATGTTCTATAGATGCTTGACCACAAGCTTTTCGACCATGTGTGATTCCACAGCTAGAGAACAGAACAATATCCAGGTCTTCTCCGCCATATTGCTCAATGAAGTGAATACTTTCAGCTCCATTTATCGGTATCCGTCTTTGTGCATAACCAGGTGAGTACTTCATTCGAATCCATCCATCCGCCTGAGAACAGTGGTTCAGTTTTGGGTCTAAACTTTGTATATAAACAAAGTTACATACATATATAACATCCGAGTTAGTTTTGTTCAGTCGTATCTTGCGTTTTTTTTCCGG

mRNA sequence

GTTGAGCCTCCCTCATACGTGCTTCCGATGTCAATTGGTGTCGGCTGGTTACATCTGCTTGTAAAGAAGGTTCTAACCTGGTTATAAACACATCCATGAGCACACTTTCAGCCATGTCCGTAAGAGGGCTGAGTAATTTAAGAGTTTCTTAACATAATCACTGTAGGATCCATCTTGGGTAATCCTAATTAGTTCTGCTCCCAAGCTCCCTTGTTCAATGTCCCTAAAATGTTGGAAAATCCTCTTTCAAATCCGACCATGATTCAACTTTCCTTCGATTGTGGCTCTATCTATACCAGTCCACTTCATCTTGGCCAAAACTCACCACAGCAACTTTGACCTTTTCAGCTTCTGGAAGATCATTAATCTAAAAAAAAATGTCCTGCACAGTAAACCCATGATTCGGGGTTCTTTACAAGGAACAGGGGCATTTCCAGCTTTTTATACTTCTATCCACAGTGACTGTCGTGTTGTCCAAACGAGTTTACAAGTCACCCATTTTCCCCTTTTGTTTCATCACAGATCCATCGGACATTCCAGATTCATATCTCCTTCGATATAATCTATTTTCTTTCATGTTATCAGCCATACGGTCAACTGCCTTCCTCAACTCGAGCATCATTTCCTTCAAACCCAGAACTTTTTCAGTACCTTCCAAGCACTCTTCCATTTGTTTATTCACCATTAATCTTGTCACAACCCCTAGATTTATGGCTCTGATACCAATATGTTAGGATTCCTCTCTTAAAAGACAATAATAATCCCAACAAAATGATAGAAACAGAGGCAGCACTCTGTCATTTACTTATATTCAATAAACAGAAAGTTCAACAAGGAAAATATCAAGAGAAAAGTAAATAAACAGCATGAAATACACACCAGACCAGCAAAGAAAGGTACAGAAATAGCCCATCCTTAATATGGCTGACGGCTCCTCCCTAAAATTATTCTATTTATACTCATCATTTGTGGGCCCTGCCAGCATCATTCTTTTCTTTAATACTCTCTCCCAGCTCTGTCCCCTATACAATTACCTTCTTACTTTTTCTTTTCTACTGATGAATAATAGGAGGCCTTGCAATGTGCTGCAGTGAGAACATCACCCTATAGATAGGAAGGAAGGAAATGGACAACATAAGGGAACGAAAAACTTCTAAAAGGTGACGGTTCTTTTGCTTATAAACCCCATTTTGATGGAGGGTGTAAGCACAGACACTTAGGTGAATAATTCCTTTAGATGACAAATATTAAACCACCAATTCATCCAAAAGCTTAAGCTAGTGGTTGAAGGCAAATTTAATTATATATCACCAACAACAAAAACTCATTAAGGGTGTGGTTTTGGAACTCACGACCATTATCACATCGTAGGTTTCAATTTTTGTATTGAATTACATTTCTACGATGTGATAGAAGTTCCAAAATGTGGAAGTTCCAGATTTGTTGAAGATAAGGAAAACCCAAGTAAGATGTTAAGGTCGTCAATAAAGGTCACAAACCACCGTTTTCCAGGGGAGGTACCGACCTTGGATGGTCCCCAAACATCACTATGAACAAGAGTGAAAAGTTGGATTGGCTTGTATGGTGGTGAGAGAAAAGAGACTCGATGCTATGTAGCCCCAATGCACACATCACAAGTTAAATAAGAAACATCAACTTTAGAAAAAAGATGAGAAAGTAAGTCCTTCATATATTGAAAGTTGGGGTGGCCTAAATGAAAATATCACAACATACAGTCTTGAAAATAAAAGACTAGTCCTAGAAATACTACTAGAGGAGGCATCATCATCAAGCAGGTAGAGTCCCCTATCGTGTCAGCCAGTGCCAATCATCTTCCCGAAGCTCAAGTCCTGAAAAGAAACATAATCAAGTAAGAATTTTGTTTTGTAGTTGAATCCACTTGTAATCTTGTTTATTGATTGAAGATTATAGGAAATTCTGCACATGCAACACATTGTGTAAGGTTAATCCTTCAAAAGGAGAAACCTTTTCCCTTCCCGGCAATGAGAGCCAAGGAGCCATTTGCAATTCTTGTTTTCTCACTTTTAGCACACAGAATGTAGGGCATAGAATGCTCACAAAAACTTGTCAAATGATTTGTGGCGCCAGAGTCTAAGAAACCCATCAACACTAATAATAAGACTGAAGGACTGAGATATACCTGTATGGTAAATAGCTCCAAGTGTAGTCGGGCTGGGATCCCTAAGTGGCTGAGAAGGGTCGGCACACTCATTCACATAAGCCCATCCTGGGTTTTGTTTGTCGTTGGAAGTGCGTCTCTTCCTTTGGCGAACACCATGTAACTTCCTGATTCAAGTCGAAGAAATCTCGCAGCTTTGTTACCAATTAATGTAAAGCAAAGAAGAATCTTATTCAAAATGTCAATTAATTCTTACAAAACAGAACAGCCGAGAATATATATGTCTACTCGATAGCCAACCTACAACCTGCTGGACAGGTTGTAAACAAACCAAAGACGTGTGTTAACCCTTACAAAACAGACATTTAATAAGTTGGGATTAAGTAAGCAACTACAATAACAGAACATACAAAAACTTTCAGCATACATCAACTACATCATCATTTTGTTCCGTTGTGAACTTTTCAGAAATGAGGGGAGCACTAGTGTCTTGATTTGTTCACGAGGAGGTGGGCTATTGTTGGAACGCATGGAACTTGTTGTGGAGCTATGGGAGGAGAAGATTAAGGCTCAGCTTGTCCCTACACCTGATCCCAGCCTAACGGAGCAATATGAATATGCTAACGAGCATGACATAAAATGTCTTGTTATTATTACGGACTCTGGTGTATCTAACACAGGTTCAGTAAAGGTTCGACACCTAGAACTCAAAAAGGAGAAGAAAGTTGAACGAACATACATTGTCAAGTTTCTCCAGGAGGCAATGGCCAATCAGTTTAAAAATCCTCTCATTTGGAATTAGAACTGTTCTTCATTGCATTACTCCGGCTTCTACTCATCCGATGTTCTATAGATGCTTGACCACAAGCTTTTCGACCATGTGTGATTCCACAGCTAGAGAACAGAACAATATCCAGGTCTTCTCCGCCATATTGCTCAATGAAGTGAATACTTTCAGCTCCATTTATCGGTATCCGTCTTTGTGCATAACCAGGTGAGTACTTCATTCGAATCCATCCATCCGCCTGAGAACAGTGGTTCAGTTTTGGGTCTAAACTTTGTATATAAACAAAGTTACATACATATATAACATCCGAGTTAGTTTTGTTCAGTCGTATCTTGCGTTTTTTTTCCGG

Coding sequence (CDS)

ATGGAACTTGTTGTGGAGCTATGGGAGGAGAAGATTAAGGCTCAGCTTGTCCCTACACCTGATCCCAGCCTAACGGAGCAATATGAATATGCTAACGAGCATGACATAAAATGTCTTGTTATTATTACGGACTCTGGTGTATCTAACACAGGTTCAGTAAAGGTTCGACACCTAGAACTCAAAAAGGAGAAGAAAGTTGAACGAACATACATTGTCAAGTTTCTCCAGGAGGCAATGGCCAATCAGTTTAAAAATCCTCTCATTTGGAATTAG

Protein sequence

MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLELKKEKKVERTYIVKFLQEAMANQFKNPLIWN*

Homology

[Close]

BLAST of CSPI06G03660 vs. ExPASy Swiss-Prot
Match: Q9LX30 (eIF-2-alpha kinase GCN2 OS=Arabidopsis thaliana OX=3702 GN=GCN2 PE=2 SV=2)

HSP 1 Score: 131.7 bits (330), Expect = 4.1e-30
Identity = 66/92 (71.74%), Postives = 76/92 (82.61%), Query Frame = 0


Query: 1    MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNT--GSVKVRHL 60
            MELV ELWE+ IKA+ VPTPDPSLTEQYEYANEH+IKCLVIIT+SGV+      VKVRHL
Sbjct: 1150 MELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVAQNQIEFVKVRHL 1209

Query: 61   ELKKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
            ELKKEK V R  +VKFL +AMA QF+NP +W+
Sbjct: 1210 ELKKEKVVGREELVKFLLDAMAVQFRNPSVWS 1241

[Close]

BLAST of CSPI06G03660 vs. ExPASy Swiss-Prot
Match: Q99KK9 (Histidine--tRNA ligase, mitochondrial OS=Mus musculus OX=10090 GN=Hars2 PE=1 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 6.6e-04
Identity = 23/80 (28.75%), Postives = 46/80 (57.50%), Query Frame = 0


Query: 1   MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 60
           ++++ ELW+  IKA+++   +P L  Q  Y  + DI  +VII +    N G +K+R +  
Sbjct: 425 LKIIAELWDAGIKAEMLYKNNPKLLTQLHYCEKADIPLMVIIGEQ-ERNEGVIKLRSVAS 484

Query: 61  KKEKKVERTYIVKFLQEAMA 81
           ++E  + R  +V  +Q+ ++
Sbjct: 485 REEVTINRESLVAEIQKRLS 503

[Close]

BLAST of CSPI06G03660 vs. ExPASy TrEMBL
Match: A0A0A0KB20 (HGTP_anticodon2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G042430 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.4e-43
Identity = 90/90 (100.00%), Postives = 90/90 (100.00%), Query Frame = 0


Query: 1   MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 60
           MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL
Sbjct: 36  MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 95

Query: 61  KKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
           KKEKKVERTYIVKFLQEAMANQFKNPLIWN
Sbjct: 96  KKEKKVERTYIVKFLQEAMANQFKNPLIWN 125

[Close]

BLAST of CSPI06G03660 vs. ExPASy TrEMBL
Match: A0A1S4DWM3 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103488674 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 4.1e-41
Identity = 87/90 (96.67%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 1   MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 60
           MELVVELWEEKIKAQLVPTPDPS TEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL
Sbjct: 864 MELVVELWEEKIKAQLVPTPDPSFTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 923

Query: 61  KKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
           KKEK+VERT IVKFLQEAMANQFKNPLIWN
Sbjct: 924 KKEKEVERTSIVKFLQEAMANQFKNPLIWN 953

[Close]

BLAST of CSPI06G03660 vs. ExPASy TrEMBL
Match: A0A1S3BDF8 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103488674 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 4.1e-41
Identity = 87/90 (96.67%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 1    MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 60
            MELVVELWEEKIKAQLVPTPDPS TEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL
Sbjct: 1016 MELVVELWEEKIKAQLVPTPDPSFTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 1075

Query: 61   KKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
            KKEK+VERT IVKFLQEAMANQFKNPLIWN
Sbjct: 1076 KKEKEVERTSIVKFLQEAMANQFKNPLIWN 1105

[Close]

BLAST of CSPI06G03660 vs. ExPASy TrEMBL
Match: A0A1S3BCY3 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103488674 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 4.1e-41
Identity = 87/90 (96.67%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 1    MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 60
            MELVVELWEEKIKAQLVPTPDPS TEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL
Sbjct: 1127 MELVVELWEEKIKAQLVPTPDPSFTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 1186

Query: 61   KKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
            KKEK+VERT IVKFLQEAMANQFKNPLIWN
Sbjct: 1187 KKEKEVERTSIVKFLQEAMANQFKNPLIWN 1216

[Close]

BLAST of CSPI06G03660 vs. ExPASy TrEMBL
Match: A0A1S3BE99 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103488674 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 4.1e-41
Identity = 87/90 (96.67%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 1    MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 60
            MELVVELWEEKIKAQLVPTPDPS TEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL
Sbjct: 1149 MELVVELWEEKIKAQLVPTPDPSFTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 1208

Query: 61   KKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
            KKEK+VERT IVKFLQEAMANQFKNPLIWN
Sbjct: 1209 KKEKEVERTSIVKFLQEAMANQFKNPLIWN 1238

[Close]

BLAST of CSPI06G03660 vs. NCBI nr
Match: XP_011656557.1 (eIF-2-alpha kinase GCN2 isoform X2 [Cucumis sativus] >XP_031743596.1 eIF-2-alpha kinase GCN2 isoform X2 [Cucumis sativus] >XP_031743597.1 eIF-2-alpha kinase GCN2 isoform X2 [Cucumis sativus] >XP_031743598.1 eIF-2-alpha kinase GCN2 isoform X2 [Cucumis sativus])

HSP 1 Score: 183.3 bits (464), Expect = 9.1e-43
Identity = 90/90 (100.00%), Postives = 90/90 (100.00%), Query Frame = 0


Query: 1    MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 60
            MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL
Sbjct: 1015 MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 1074

Query: 61   KKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
            KKEKKVERTYIVKFLQEAMANQFKNPLIWN
Sbjct: 1075 KKEKKVERTYIVKFLQEAMANQFKNPLIWN 1104

[Close]

BLAST of CSPI06G03660 vs. NCBI nr
Match: XP_011656554.1 (eIF-2-alpha kinase GCN2 isoform X1 [Cucumis sativus] >XP_011656555.1 eIF-2-alpha kinase GCN2 isoform X1 [Cucumis sativus] >KAE8646623.1 hypothetical protein Csa_005478 [Cucumis sativus])

HSP 1 Score: 183.3 bits (464), Expect = 9.1e-43
Identity = 90/90 (100.00%), Postives = 90/90 (100.00%), Query Frame = 0


Query: 1    MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 60
            MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL
Sbjct: 1148 MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 1207

Query: 61   KKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
            KKEKKVERTYIVKFLQEAMANQFKNPLIWN
Sbjct: 1208 KKEKKVERTYIVKFLQEAMANQFKNPLIWN 1237

[Close]

BLAST of CSPI06G03660 vs. NCBI nr
Match: XP_008445739.1 (PREDICTED: eIF-2-alpha kinase GCN2 isoform X2 [Cucumis melo])

HSP 1 Score: 176.8 bits (447), Expect = 8.5e-41
Identity = 87/90 (96.67%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 1    MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 60
            MELVVELWEEKIKAQLVPTPDPS TEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL
Sbjct: 1127 MELVVELWEEKIKAQLVPTPDPSFTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 1186

Query: 61   KKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
            KKEK+VERT IVKFLQEAMANQFKNPLIWN
Sbjct: 1187 KKEKEVERTSIVKFLQEAMANQFKNPLIWN 1216

[Close]

BLAST of CSPI06G03660 vs. NCBI nr
Match: XP_008445742.1 (PREDICTED: eIF-2-alpha kinase GCN2 isoform X3 [Cucumis melo])

HSP 1 Score: 176.8 bits (447), Expect = 8.5e-41
Identity = 87/90 (96.67%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 1    MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 60
            MELVVELWEEKIKAQLVPTPDPS TEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL
Sbjct: 1016 MELVVELWEEKIKAQLVPTPDPSFTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 1075

Query: 61   KKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
            KKEK+VERT IVKFLQEAMANQFKNPLIWN
Sbjct: 1076 KKEKEVERTSIVKFLQEAMANQFKNPLIWN 1105

[Close]

BLAST of CSPI06G03660 vs. NCBI nr
Match: XP_008445738.1 (PREDICTED: eIF-2-alpha kinase GCN2 isoform X1 [Cucumis melo])

HSP 1 Score: 176.8 bits (447), Expect = 8.5e-41
Identity = 87/90 (96.67%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 1    MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 60
            MELVVELWEEKIKAQLVPTPDPS TEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL
Sbjct: 1149 MELVVELWEEKIKAQLVPTPDPSFTEQYEYANEHDIKCLVIITDSGVSNTGSVKVRHLEL 1208

Query: 61   KKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
            KKEK+VERT IVKFLQEAMANQFKNPLIWN
Sbjct: 1209 KKEKEVERTSIVKFLQEAMANQFKNPLIWN 1238

[Close]

BLAST of CSPI06G03660 vs. TAIR 10
Match: AT3G59410.1 (protein kinase family protein )

HSP 1 Score: 131.7 bits (330), Expect = 2.9e-31
Identity = 66/92 (71.74%), Postives = 76/92 (82.61%), Query Frame = 0


Query: 1    MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNT--GSVKVRHL 60
            MELV ELWE+ IKA+ VPTPDPSLTEQYEYANEH+IKCLVIIT+SGV+      VKVRHL
Sbjct: 1150 MELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVAQNQIEFVKVRHL 1209

Query: 61   ELKKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
            ELKKEK V R  +VKFL +AMA QF+NP +W+
Sbjct: 1210 ELKKEKVVGREELVKFLLDAMAVQFRNPSVWS 1241

[Close]

BLAST of CSPI06G03660 vs. TAIR 10
Match: AT3G59410.2 (protein kinase family protein )

HSP 1 Score: 131.7 bits (330), Expect = 2.9e-31
Identity = 66/92 (71.74%), Postives = 76/92 (82.61%), Query Frame = 0


Query: 1    MELVVELWEEKIKAQLVPTPDPSLTEQYEYANEHDIKCLVIITDSGVSNT--GSVKVRHL 60
            MELV ELWE+ IKA+ VPTPDPSLTEQYEYANEH+IKCLVIIT+SGV+      VKVRHL
Sbjct: 1174 MELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVAQNQIEFVKVRHL 1233

Query: 61   ELKKEKKVERTYIVKFLQEAMANQFKNPLIWN 91
            ELKKEK V R  +VKFL +AMA QF+NP +W+
Sbjct: 1234 ELKKEKVVGREELVKFLLDAMAVQFRNPSVWS 1265

The following BLAST results are available for this feature:

BLAST of CSPI06G03660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LX30	4.1e-30	71.74	eIF-2-alpha kinase GCN2 OS=Arabidopsis thaliana OX=3702 GN=GCN2 PE=2 SV=2	[more]
Q99KK9	6.6e-04	28.75	Histidine--tRNA ligase, mitochondrial OS=Mus musculus OX=10090 GN=Hars2 PE=1 SV=...	[more]

BLAST of CSPI06G03660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KB20	4.4e-43	100.00	HGTP_anticodon2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G04...	[more]
A0A1S4DWM3	4.1e-41	96.67	Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A1S3BDF8	4.1e-41	96.67	Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A1S3BCY3	4.1e-41	96.67	Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A1S3BE99	4.1e-41	96.67	Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348...	[more]

BLAST of CSPI06G03660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011656557.1	9.1e-43	100.00	eIF-2-alpha kinase GCN2 isoform X2 [Cucumis sativus] >XP_031743596.1 eIF-2-alpha...	[more]
XP_011656554.1	9.1e-43	100.00	eIF-2-alpha kinase GCN2 isoform X1 [Cucumis sativus] >XP_011656555.1 eIF-2-alpha...	[more]
XP_008445739.1	8.5e-41	96.67	PREDICTED: eIF-2-alpha kinase GCN2 isoform X2 [Cucumis melo]	[more]
XP_008445742.1	8.5e-41	96.67	PREDICTED: eIF-2-alpha kinase GCN2 isoform X3 [Cucumis melo]	[more]
XP_008445738.1	8.5e-41	96.67	PREDICTED: eIF-2-alpha kinase GCN2 isoform X1 [Cucumis melo]	[more]

BLAST of CSPI06G03660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G59410.1	2.9e-31	71.74	protein kinase family protein	[more]
AT3G59410.2	2.9e-31	71.74	protein kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036621	Anticodon-binding domain superfamily	GENE3D	3.40.50.800		coord: 1..82 e-value: 1.2E-16 score: 62.4
IPR024435	Histidyl tRNA synthetase-related domain	PFAM	PF12745	HGTP_anticodon2	coord: 2..82 e-value: 3.5E-8 score: 33.3
None	No IPR available	PANTHER	PTHR11476:SF10	EIF-2-ALPHA KINASE GCN2	coord: 1..90
None	No IPR available	PANTHER	PTHR11476	HISTIDYL-TRNA SYNTHETASE	coord: 1..90
None	No IPR available	SUPERFAMILY	52954	Class II aaRS ABD-related	coord: 2..81

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G03660.1	CSPI06G03660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018105	peptidyl-serine phosphorylation
biological_process	GO:0006521	regulation of cellular amino acid metabolic process
biological_process	GO:0010998	regulation of translational initiation by eIF2 alpha phosphorylation
biological_process	GO:0009635	response to herbicide
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004694	eukaryotic translation initiation factor 2alpha kinase activity
molecular_function	GO:0106310	protein serine kinase activity
molecular_function	GO:0004712	protein serine/threonine/tyrosine kinase activity
molecular_function	GO:0000049	tRNA binding