CSPI06G03050 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G03050
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionadenylate isopentenyltransferase-like
LocationChr6: 2519180 .. 2520269 (+)
RNA-Seq ExpressionCSPI06G03050
SyntenyCSPI06G03050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGGTAACCGTCGTCGTACGTAAAGAAAAAGGACTGATATTCCTCCGTTCCGTATATGTCTCTTCACATCAATAAACATTACCTCATCATCATCATCATCATCATCATCATTCACATTCTATGACACTTTCCCTCCCTTTCTCCCACTCTTTTCTTCTTTCCCGCCGCCCCCGATGCCCTCTTATCCTCTCCTCTGCACCCTCCGATTCCCTCCTCCTACTTCTTGGCGCCACCGGCTCCGGCAAGTCTTCCCTCTCCATCGACCTCGCCGCTCGCTTCCCTTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACAGAGGCCTCGATATCACCACTAACAAGCTTCCCCTCTCCCACCGCCGCGCGGTTCCTCATCATCTACTTGGCGATTTCGACCCTCTAGCTGGCGAGGTTTCTCCTGCTGCTTTCCGCTCCCATGCCTCCGCCGTCATCTCCGGCGTTATCTCCCGAGGTAACCTTCCACTAGTCGTTGGCGGCTCCAACTCCTTCGTTCACGCTCTTGTCGCCGCTGATTTCGATGCTACCCAAGACGTTTTCTGTGGGTCTAGGAATCGGGTGTCGACGGAGTTTCTGTACCGGTGCTGCTTTCTGTGGGTGGACGTGTCGTTTCCTGTTTTGAAGCGATATTTGTCGAAGCGGGTGGATGAGATGTTGGATGGTGGGATGGTGGAGGAGTTGGCGAAGTTTTATGACCCGGAAATGGCCGATTCGGAGACACGAGTGGGGCTGAGGAAAGCCATTGGAGTGCCAGAATTCGAGGAGTATTTCCGGCGGCATCACCCGAAAGGGAGGGATTACAGAGAGGGGGATCCGCTGCAGGCAAGTGCATTTGAAGAGGCGGTAAGGAAAATAAAAGAGAACACGTGGCAACTGACGAAAAGGCAGTTGTGGAAGATCGGACGGCTTAGAAGGGCTGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTGCTAGCGTCGGAAGTGGCGGAGAAACGGTCAGAAATATGGGAGACACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGGGCTTCATTCAGGAGTAGGTTTTCC

mRNA sequence

CCGGTAACCGTCGTCGTACGTAAAGAAAAAGGACTGATATTCCTCCGTTCCGTATATGTCTCTTCACATCAATAAACATTACCTCATCATCATCATCATCATCATCATCATTCACATTCTATGACACTTTCCCTCCCTTTCTCCCACTCTTTTCTTCTTTCCCGCCGCCCCCGATGCCCTCTTATCCTCTCCTCTGCACCCTCCGATTCCCTCCTCCTACTTCTTGGCGCCACCGGCTCCGGCAAGTCTTCCCTCTCCATCGACCTCGCCGCTCGCTTCCCTTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACAGAGGCCTCGATATCACCACTAACAAGCTTCCCCTCTCCCACCGCCGCGCGGTTCCTCATCATCTACTTGGCGATTTCGACCCTCTAGCTGGCGAGGTTTCTCCTGCTGCTTTCCGCTCCCATGCCTCCGCCGTCATCTCCGGCGTTATCTCCCGAGGTAACCTTCCACTAGTCGTTGGCGGCTCCAACTCCTTCGTTCACGCTCTTGTCGCCGCTGATTTCGATGCTACCCAAGACGTTTTCTGTGGGTCTAGGAATCGGGTGTCGACGGAGTTTCTGTACCGGTGCTGCTTTCTGTGGGTGGACGTGTCGTTTCCTGTTTTGAAGCGATATTTGTCGAAGCGGGTGGATGAGATGTTGGATGGTGGGATGGTGGAGGAGTTGGCGAAGTTTTATGACCCGGAAATGGCCGATTCGGAGACACGAGTGGGGCTGAGGAAAGCCATTGGAGTGCCAGAATTCGAGGAGTATTTCCGGCGGCATCACCCGAAAGGGAGGGATTACAGAGAGGGGGATCCGCTGCAGGCAAGTGCATTTGAAGAGGCGGTAAGGAAAATAAAAGAGAACACGTGGCAACTGACGAAAAGGCAGTTGTGGAAGATCGGACGGCTTAGAAGGGCTGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTGCTAGCGTCGGAAGTGGCGGAGAAACGGTCAGAAATATGGGAGACACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGGGCTTCATTCAGGAGTAGGTTTTCC

Coding sequence (CDS)

ATGACACTTTCCCTCCCTTTCTCCCACTCTTTTCTTCTTTCCCGCCGCCCCCGATGCCCTCTTATCCTCTCCTCTGCACCCTCCGATTCCCTCCTCCTACTTCTTGGCGCCACCGGCTCCGGCAAGTCTTCCCTCTCCATCGACCTCGCCGCTCGCTTCCCTTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACAGAGGCCTCGATATCACCACTAACAAGCTTCCCCTCTCCCACCGCCGCGCGGTTCCTCATCATCTACTTGGCGATTTCGACCCTCTAGCTGGCGAGGTTTCTCCTGCTGCTTTCCGCTCCCATGCCTCCGCCGTCATCTCCGGCGTTATCTCCCGAGGTAACCTTCCACTAGTCGTTGGCGGCTCCAACTCCTTCGTTCACGCTCTTGTCGCCGCTGATTTCGATGCTACCCAAGACGTTTTCTGTGGGTCTAGGAATCGGGTGTCGACGGAGTTTCTGTACCGGTGCTGCTTTCTGTGGGTGGACGTGTCGTTTCCTGTTTTGAAGCGATATTTGTCGAAGCGGGTGGATGAGATGTTGGATGGTGGGATGGTGGAGGAGTTGGCGAAGTTTTATGACCCGGAAATGGCCGATTCGGAGACACGAGTGGGGCTGAGGAAAGCCATTGGAGTGCCAGAATTCGAGGAGTATTTCCGGCGGCATCACCCGAAAGGGAGGGATTACAGAGAGGGGGATCCGCTGCAGGCAAGTGCATTTGAAGAGGCGGTAAGGAAAATAAAAGAGAACACGTGGCAACTGACGAAAAGGCAGTTGTGGAAGATCGGACGGCTTAGAAGGGCTGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTGCTAGCGTCGGAAGTGGCGGAGAAACGGTCAGAAATATGGGAGACACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGGGCTTCATTCAGGAGTAG

Protein sequence

MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIVKGFIQE*
Homology
BLAST of CSPI06G03050 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.7e-86
Identity = 175/301 (58.14%), Postives = 217/301 (72.09%), Query Frame = 0

Query: 29  DSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHL 88
           + LL+L+GATG+GKS LSIDLAA FP E+INSDKMQ+Y+GLDITTNK+ +  R  VPHHL
Sbjct: 30  EKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPHHL 89

Query: 89  LGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDAT-QDVF 148
           LG+ DP  GE++PA FRS A   +S +  R  LP++VGGSNSF+HAL+   FD++   VF
Sbjct: 90  LGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPGVF 149

Query: 149 -CGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPE----M 208
             GS + VS+E  Y CCFLWVDVS  VL  YL+KRVD+ML+ GM +ELA+FY PE     
Sbjct: 150 EEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDEDHD 209

Query: 209 ADSETRVGLRKAIGVPEFEEYFRRHHP---KGRDYREGDPLQASAFEEAVRKIKENTWQL 268
            DS TR GLRKAIGVPEF+ YF +  P   +G D    D ++  AFEEAVR IKENT  L
Sbjct: 210 EDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGR-DRVRRGAFEEAVRAIKENTCHL 269

Query: 269 TKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIVKGFIQ 321
            KRQ+ KI RL+ AGWDL+R+DAT +  A + S+  EK +EIWE QV+EPSVKIV  F+ 
Sbjct: 270 AKRQIGKILRLKGAGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRFLD 329

BLAST of CSPI06G03050 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 310.5 bits (794), Expect = 2.3e-83
Identity = 165/315 (52.38%), Postives = 217/315 (68.89%), Query Frame = 0

Query: 10  SFLLSRRPRCPLI--------LSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSD 69
           SF   RR   PL+         S    D ++++LGATG+GKS LS+DLA RFPSEIINSD
Sbjct: 38  SFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSD 97

Query: 70  KMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGNL 129
           K+Q+Y GL+ITTN++ L  RR VPHHLLG  +P  GE++   FRS AS V+  + SR  +
Sbjct: 98  KIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKV 157

Query: 130 PLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKR 189
           P++ GGSNSFVHAL+A  FD   D F      +S++  Y CCF+WVDVS  VL  YL +R
Sbjct: 158 PIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRR 217

Query: 190 VDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQ 249
           VDEM+D GM EEL++FYDP  +  ETR G+RKAIGVPEF+ YF+ + P+ +   + D L+
Sbjct: 218 VDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPE-KKMIKWDALR 277

Query: 250 ASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKR-SE 309
            +A+++AV  IK NTW L KRQ+ KI  L+ AGW+++RVDATA+  AV+    +EK+  E
Sbjct: 278 KAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDATASFKAVMMKSSSEKKWRE 337

Query: 310 IWETQVVEPSVKIVK 316
            WE QV+EPSVKIVK
Sbjct: 338 NWEEQVLEPSVKIVK 351

BLAST of CSPI06G03050 vs. ExPASy Swiss-Prot
Match: Q9C6L1 (Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT6 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.3e-73
Identity = 161/336 (47.92%), Postives = 220/336 (65.48%), Query Frame = 0

Query: 3   LSLPFSHSFLL---SRRPRCPLILSSAP--------SDSLLLLLGATGSGKSSLSIDLAA 62
           LS P SHS LL   + +   P ++++           D ++L+ G TG+GKS LS+DLA 
Sbjct: 8   LSPPLSHSSLLPTVTTKFGSPRLVTTCMGHAGRKNIKDKVVLITGTTGTGKSRLSVDLAT 67

Query: 63  R-FPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASA 122
           R FP+EIINSDKMQ+Y+G +I TN +PL  +  VPHHLLG F P  GE++PA FRS A+ 
Sbjct: 68  RFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLLGQFHPQDGELTPAEFRSLATL 127

Query: 123 VISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFC--GSRNRVSTEFLYRCCFLWVD 182
            IS +IS   LP+VVGGSNSF HAL+A  FD   D F    S + + ++  Y+CC LWVD
Sbjct: 128 SISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFSPGSSLSTICSDLRYKCCILWVD 187

Query: 183 VSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHH 242
           V  PVL ++L  RVD+M++ G+VE+LA+ YDP + DS  R+G+RK IGV EF+ YF R +
Sbjct: 188 VLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGRRLGVRKTIGVEEFDRYF-RVY 247

Query: 243 PKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVA 302
           PK  D    D  + +A+EE V+ +KE T +L K+Q  KI +L R GW++KR+DATAA++A
Sbjct: 248 PKEMDKGIWDLARKAAYEETVKGMKERTCRLVKKQKEKIMKLIRGGWEIKRLDATAAIMA 307

Query: 303 VLASEVAEKRS----EIWETQVVEPSVKIVKGFIQE 321
            L    A+       EIWE  +V+ SV+IVK F+ E
Sbjct: 308 ELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

BLAST of CSPI06G03050 vs. ExPASy Swiss-Prot
Match: Q9SB60 (Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.8e-73
Identity = 146/298 (48.99%), Postives = 202/298 (67.79%), Query Frame = 0

Query: 28  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 87
           +D +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+  +  RR VPHH
Sbjct: 4   NDKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHH 63

Query: 88  LLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVF 147
           LLG+ +P AGEV+ A FR  A+  IS +  R  LP++ GGSNS++HAL+A  +D     F
Sbjct: 64  LLGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPF 123

Query: 148 CGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSET 207
              +  + +E  Y CCF+W+DV   VL  YLS R+D M+  GM EE+A+F+  + A  E 
Sbjct: 124 SDHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP 183

Query: 208 RVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKI 267
            +G+ KAIGV EF++Y +  +    D  + DP++  A+E+AVR IKENT+QLTK Q+ KI
Sbjct: 184 -LGIWKAIGVQEFDDYLKM-YKWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKI 243

Query: 268 GRLRRAGWDLKRVDATA----AVVAVLASE-VAEKRSEIWETQVVEPSVKIVKGFIQE 321
            +LR AGWD+K+VDATA    A+ A    E VAE + +IW  +V+EP VKIV+  + +
Sbjct: 244 NKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

BLAST of CSPI06G03050 vs. ExPASy Swiss-Prot
Match: Q9LJL4 (Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT8 PE=2 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 8.1e-73
Identity = 141/304 (46.38%), Postives = 199/304 (65.46%), Query Frame = 0

Query: 17  PRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKL 76
           P   + +  +    +++++GATGSGKS LSIDLA RF  EI+NSDK+Q Y GL +TTN++
Sbjct: 31  PMTTVCMEQSYKQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQM 90

Query: 77  PLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALV 136
            +  R  VPHHLLG+  P   E++ + FRS AS  IS + +RGNLP++ GGSNSF+HAL+
Sbjct: 91  SILERCGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALL 150

Query: 137 AADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAK 196
              FD     F  S   +S+   Y CCFLWVDVS  VL  YLSKRVD+M++ GM EELA 
Sbjct: 151 VDRFDPKTYPF-SSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAG 210

Query: 197 FYDPEMADSETRV-GLRKAIGVPEFEEYFRRHHPKGRD-YREGDPLQASAFEEAVRKIKE 256
           FYDP  + S  R  G+ K IG+PEF+ YF  + P+ +    E D  +  A++EAV++IKE
Sbjct: 211 FYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKE 270

Query: 257 NTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIV 316
           NTW+L K+Q+ +I +L+ +GWD++R+DAT +              EIW+  V++ S+K+V
Sbjct: 271 NTWRLAKKQIERIMKLKSSGWDIQRLDATPS--------FGRSSREIWDNTVLDESIKVV 325

Query: 317 KGFI 319
           K F+
Sbjct: 331 KRFL 325

BLAST of CSPI06G03050 vs. ExPASy TrEMBL
Match: A0A0A0K943 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G030440 PE=4 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 3.7e-177
Identity = 319/320 (99.69%), Postives = 319/320 (99.69%), Query Frame = 0

Query: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180
           LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK
Sbjct: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240
           RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300
           QA AFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE
Sbjct: 241 QARAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300

Query: 301 IWETQVVEPSVKIVKGFIQE 321
           IWETQVVEPSVKIVKGFIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

BLAST of CSPI06G03050 vs. ExPASy TrEMBL
Match: A0A5D3CF13 (Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold383G00180 PE=4 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.1e-172
Identity = 309/320 (96.56%), Postives = 314/320 (98.12%), Query Frame = 0

Query: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSF LSRRPRCPL+LSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFPLSRRPRCPLVLSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGD+DPLAGEVSPAAFRSHASAVISGVISRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180
           LPLVVGGSNSF+HALVAADFD TQDVFCGSRNRVS EF YRCCFLWVDVSFPVLKRYLSK
Sbjct: 121 LPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240
           RVDEMLDGGM+EELA+FYDPEMAD ETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300
           QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASE AEKRSE
Sbjct: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300

Query: 301 IWETQVVEPSVKIVKGFIQE 321
           IWETQVVEPSVKIVKGFIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

BLAST of CSPI06G03050 vs. ExPASy TrEMBL
Match: A0A1S3BD73 (adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103488599 PE=4 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.1e-172
Identity = 309/320 (96.56%), Postives = 314/320 (98.12%), Query Frame = 0

Query: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSF LSRRPRCPL+LSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFPLSRRPRCPLVLSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGD+DPLAGEVSPAAFRSHASAVISGVISRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180
           LPLVVGGSNSF+HALVAADFD TQDVFCGSRNRVS EF YRCCFLWVDVSFPVLKRYLSK
Sbjct: 121 LPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240
           RVDEMLDGGM+EELA+FYDPEMAD ETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300
           QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASE AEKRSE
Sbjct: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300

Query: 301 IWETQVVEPSVKIVKGFIQE 321
           IWETQVVEPSVKIVKGFIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

BLAST of CSPI06G03050 vs. ExPASy TrEMBL
Match: A0A6J1FLX4 (adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111445165 PE=4 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.1e-144
Identity = 274/330 (83.03%), Postives = 294/330 (89.09%), Query Frame = 0

Query: 1   MTLSLPFSHSFL----------LSRRPRCPLIL-SSAPSDSLLLLLGATGSGKSSLSIDL 60
           MTLSL FSHS L           SRRPR PL+L SS+PS +L+LLLGATGSGKSSLSIDL
Sbjct: 43  MTLSLSFSHSSLHSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDL 102

Query: 61  AARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHAS 120
           A+RFPSEIINSDKMQLY GLDITTNKLPLS RRAVPHHLLGDFDPLAGEVS +AFRSHA+
Sbjct: 103 ASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAA 162

Query: 121 AVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDV 180
           AVISGVISRGNLPLVVGGSNSF+HALV ADFD + DVF  SRNRVSTEF YRCCFLWVDV
Sbjct: 163 AVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDV 222

Query: 181 SFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHP 240
           SFPVLKRYLSKRVDEML+ GMV ELA+FYDPE+ADSETRVGLRKAIGV EFEE+FRRHHP
Sbjct: 223 SFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHP 282

Query: 241 KGRDYREG-DPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVA 300
           KGR+Y+EG DP+ ASA++EAVRKIKENTW+LTKRQL KIGRLRRAGWDLKRVDATAAVVA
Sbjct: 283 KGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVA 342

Query: 301 VLASEVAEKRSEIWETQVVEPSVKIVKGFI 319
           VL SE AEKRSEIWE +VVE SVKIVK FI
Sbjct: 343 VLGSEAAEKRSEIWEAEVVEASVKIVKRFI 372

BLAST of CSPI06G03050 vs. ExPASy TrEMBL
Match: A0A6J1HF40 (adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463593 PE=4 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.6e-143
Identity = 269/334 (80.54%), Postives = 290/334 (86.83%), Query Frame = 0

Query: 1   MTLSLPFSHSFL--------------LSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLS 60
           MTLSLPFSHS L               SRR + P +LSS PSD+LLLLLGATGSGKSSLS
Sbjct: 1   MTLSLPFSHSSLHYHHHHHPFPLFLSPSRRFQWPRMLSSLPSDNLLLLLGATGSGKSSLS 60

Query: 61  IDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRS 120
           ID+AARFPSEIINSDKMQLYRGLD+TTNKLP S RR VPHHLLGDFDPLAGEVS AAFRS
Sbjct: 61  IDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSAAAFRS 120

Query: 121 HASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLW 180
            ASAVISGV+SRGNLP+VVGGSNS++HALV A FD   DVF G  NRVS EF YRCCFLW
Sbjct: 121 QASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFDQDYDVFDGPLNRVSAEFRYRCCFLW 180

Query: 181 VDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRR 240
           VDVSFPVLK+YLSKRVD+ML+ GMV ELA+FYDPEMA++E RVGLRKAIGVPEFEE+FRR
Sbjct: 181 VDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFEEFFRR 240

Query: 241 HHPKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAV 300
           +HPKGR+YREGDPL+A A+EEAVRKIKENTWQLTKRQL KIGRLR AGWDLKRVDATAAV
Sbjct: 241 YHPKGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRVDATAAV 300

Query: 301 VAVLASEVAEKRSEIWETQVVEPSVKIVKGFIQE 321
           VAVLASE AEKRSEIWE QVVEPSVKIVK FI+E
Sbjct: 301 VAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

BLAST of CSPI06G03050 vs. NCBI nr
Match: XP_004150914.1 (adenylate isopentenyltransferase [Cucumis sativus] >KGN45943.1 hypothetical protein Csa_005709 [Cucumis sativus])

HSP 1 Score: 630.6 bits (1625), Expect = 7.6e-177
Identity = 319/320 (99.69%), Postives = 319/320 (99.69%), Query Frame = 0

Query: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180
           LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK
Sbjct: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240
           RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300
           QA AFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE
Sbjct: 241 QARAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300

Query: 301 IWETQVVEPSVKIVKGFIQE 321
           IWETQVVEPSVKIVKGFIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

BLAST of CSPI06G03050 vs. NCBI nr
Match: XP_008445640.1 (PREDICTED: adenylate isopentenyltransferase-like [Cucumis melo] >KAA0049650.1 adenylate isopentenyltransferase-like [Cucumis melo var. makuwa] >TYK08909.1 adenylate isopentenyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 614.8 bits (1584), Expect = 4.3e-172
Identity = 309/320 (96.56%), Postives = 314/320 (98.12%), Query Frame = 0

Query: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSF LSRRPRCPL+LSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFPLSRRPRCPLVLSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGD+DPLAGEVSPAAFRSHASAVISGVISRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180
           LPLVVGGSNSF+HALVAADFD TQDVFCGSRNRVS EF YRCCFLWVDVSFPVLKRYLSK
Sbjct: 121 LPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240
           RVDEMLDGGM+EELA+FYDPEMAD ETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300
           QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASE AEKRSE
Sbjct: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300

Query: 301 IWETQVVEPSVKIVKGFIQE 321
           IWETQVVEPSVKIVKGFIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

BLAST of CSPI06G03050 vs. NCBI nr
Match: XP_038886381.1 (adenylate isopentenyltransferase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 543.9 bits (1400), Expect = 9.4e-151
Identity = 273/307 (88.93%), Postives = 290/307 (94.46%), Query Frame = 0

Query: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSF LSRR RCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFPLSRRRRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120
           DKMQLY GLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHA++VISGV+SRGN
Sbjct: 61  DKMQLYGGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHAASVISGVVSRGN 120

Query: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180
           LPLVVGGSNSF+HALVAADFDATQDVF GSRNR+S EF YRCCFLWVDVSFPVLKRYLSK
Sbjct: 121 LPLVVGGSNSFIHALVAADFDATQDVFGGSRNRISAEFRYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240
           RVDEML+ GMVEELA+FYDPEMA+SE R+GLRKAIGVPEFEE+FRR+HPKGRDYREGDPL
Sbjct: 181 RVDEMLESGMVEELAEFYDPEMAESEPRIGLRKAIGVPEFEEFFRRYHPKGRDYREGDPL 240

Query: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEK--- 300
           +ASA+EEAVRKIKENTWQLTKRQLWKIGRLR+AGWDLKRVDATAAVVAVLA+E A     
Sbjct: 241 RASAYEEAVRKIKENTWQLTKRQLWKIGRLRKAGWDLKRVDATAAVVAVLAAEAARNGQK 300

Query: 301 --RSEIW 303
             R+ +W
Sbjct: 301 YGRNRLW 307

BLAST of CSPI06G03050 vs. NCBI nr
Match: XP_022939170.1 (adenylate isopentenyltransferase-like [Cucurbita moschata])

HSP 1 Score: 521.2 bits (1341), Expect = 6.5e-144
Identity = 274/330 (83.03%), Postives = 294/330 (89.09%), Query Frame = 0

Query: 1   MTLSLPFSHSFL----------LSRRPRCPLIL-SSAPSDSLLLLLGATGSGKSSLSIDL 60
           MTLSL FSHS L           SRRPR PL+L SS+PS +L+LLLGATGSGKSSLSIDL
Sbjct: 43  MTLSLSFSHSSLHSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDL 102

Query: 61  AARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHAS 120
           A+RFPSEIINSDKMQLY GLDITTNKLPLS RRAVPHHLLGDFDPLAGEVS +AFRSHA+
Sbjct: 103 ASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAA 162

Query: 121 AVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDV 180
           AVISGVISRGNLPLVVGGSNSF+HALV ADFD + DVF  SRNRVSTEF YRCCFLWVDV
Sbjct: 163 AVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDV 222

Query: 181 SFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHP 240
           SFPVLKRYLSKRVDEML+ GMV ELA+FYDPE+ADSETRVGLRKAIGV EFEE+FRRHHP
Sbjct: 223 SFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHP 282

Query: 241 KGRDYREG-DPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVA 300
           KGR+Y+EG DP+ ASA++EAVRKIKENTW+LTKRQL KIGRLRRAGWDLKRVDATAAVVA
Sbjct: 283 KGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVA 342

Query: 301 VLASEVAEKRSEIWETQVVEPSVKIVKGFI 319
           VL SE AEKRSEIWE +VVE SVKIVK FI
Sbjct: 343 VLGSEAAEKRSEIWEAEVVEASVKIVKRFI 372

BLAST of CSPI06G03050 vs. NCBI nr
Match: XP_022963367.1 (adenylate isopentenyltransferase-like [Cucurbita moschata])

HSP 1 Score: 518.8 bits (1335), Expect = 3.2e-143
Identity = 269/334 (80.54%), Postives = 290/334 (86.83%), Query Frame = 0

Query: 1   MTLSLPFSHSFL--------------LSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLS 60
           MTLSLPFSHS L               SRR + P +LSS PSD+LLLLLGATGSGKSSLS
Sbjct: 1   MTLSLPFSHSSLHYHHHHHPFPLFLSPSRRFQWPRMLSSLPSDNLLLLLGATGSGKSSLS 60

Query: 61  IDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRS 120
           ID+AARFPSEIINSDKMQLYRGLD+TTNKLP S RR VPHHLLGDFDPLAGEVS AAFRS
Sbjct: 61  IDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSAAAFRS 120

Query: 121 HASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLW 180
            ASAVISGV+SRGNLP+VVGGSNS++HALV A FD   DVF G  NRVS EF YRCCFLW
Sbjct: 121 QASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFDQDYDVFDGPLNRVSAEFRYRCCFLW 180

Query: 181 VDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRR 240
           VDVSFPVLK+YLSKRVD+ML+ GMV ELA+FYDPEMA++E RVGLRKAIGVPEFEE+FRR
Sbjct: 181 VDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFEEFFRR 240

Query: 241 HHPKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAV 300
           +HPKGR+YREGDPL+A A+EEAVRKIKENTWQLTKRQL KIGRLR AGWDLKRVDATAAV
Sbjct: 241 YHPKGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRVDATAAV 300

Query: 301 VAVLASEVAEKRSEIWETQVVEPSVKIVKGFIQE 321
           VAVLASE AEKRSEIWE QVVEPSVKIVK FI+E
Sbjct: 301 VAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

BLAST of CSPI06G03050 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 310.5 bits (794), Expect = 1.6e-84
Identity = 165/315 (52.38%), Postives = 217/315 (68.89%), Query Frame = 0

Query: 10  SFLLSRRPRCPLI--------LSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSD 69
           SF   RR   PL+         S    D ++++LGATG+GKS LS+DLA RFPSEIINSD
Sbjct: 38  SFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSD 97

Query: 70  KMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGNL 129
           K+Q+Y GL+ITTN++ L  RR VPHHLLG  +P  GE++   FRS AS V+  + SR  +
Sbjct: 98  KIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKV 157

Query: 130 PLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKR 189
           P++ GGSNSFVHAL+A  FD   D F      +S++  Y CCF+WVDVS  VL  YL +R
Sbjct: 158 PIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRR 217

Query: 190 VDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQ 249
           VDEM+D GM EEL++FYDP  +  ETR G+RKAIGVPEF+ YF+ + P+ +   + D L+
Sbjct: 218 VDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPE-KKMIKWDALR 277

Query: 250 ASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKR-SE 309
            +A+++AV  IK NTW L KRQ+ KI  L+ AGW+++RVDATA+  AV+    +EK+  E
Sbjct: 278 KAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDATASFKAVMMKSSSEKKWRE 337

Query: 310 IWETQVVEPSVKIVK 316
            WE QV+EPSVKIVK
Sbjct: 338 NWEEQVLEPSVKIVK 351

BLAST of CSPI06G03050 vs. TAIR 10
Match: AT1G25410.1 (isopentenyltransferase 6 )

HSP 1 Score: 278.1 bits (710), Expect = 8.9e-75
Identity = 161/336 (47.92%), Postives = 220/336 (65.48%), Query Frame = 0

Query: 3   LSLPFSHSFLL---SRRPRCPLILSSAP--------SDSLLLLLGATGSGKSSLSIDLAA 62
           LS P SHS LL   + +   P ++++           D ++L+ G TG+GKS LS+DLA 
Sbjct: 8   LSPPLSHSSLLPTVTTKFGSPRLVTTCMGHAGRKNIKDKVVLITGTTGTGKSRLSVDLAT 67

Query: 63  R-FPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASA 122
           R FP+EIINSDKMQ+Y+G +I TN +PL  +  VPHHLLG F P  GE++PA FRS A+ 
Sbjct: 68  RFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLLGQFHPQDGELTPAEFRSLATL 127

Query: 123 VISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFC--GSRNRVSTEFLYRCCFLWVD 182
            IS +IS   LP+VVGGSNSF HAL+A  FD   D F    S + + ++  Y+CC LWVD
Sbjct: 128 SISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFSPGSSLSTICSDLRYKCCILWVD 187

Query: 183 VSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHH 242
           V  PVL ++L  RVD+M++ G+VE+LA+ YDP + DS  R+G+RK IGV EF+ YF R +
Sbjct: 188 VLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGRRLGVRKTIGVEEFDRYF-RVY 247

Query: 243 PKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVA 302
           PK  D    D  + +A+EE V+ +KE T +L K+Q  KI +L R GW++KR+DATAA++A
Sbjct: 248 PKEMDKGIWDLARKAAYEETVKGMKERTCRLVKKQKEKIMKLIRGGWEIKRLDATAAIMA 307

Query: 303 VLASEVAEKRS----EIWETQVVEPSVKIVKGFIQE 321
            L    A+       EIWE  +V+ SV+IVK F+ E
Sbjct: 308 ELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

BLAST of CSPI06G03050 vs. TAIR 10
Match: AT4G24650.1 (isopentenyltransferase 4 )

HSP 1 Score: 276.9 bits (707), Expect = 2.0e-74
Identity = 146/298 (48.99%), Postives = 202/298 (67.79%), Query Frame = 0

Query: 28  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 87
           +D +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+  +  RR VPHH
Sbjct: 4   NDKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHH 63

Query: 88  LLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVF 147
           LLG+ +P AGEV+ A FR  A+  IS +  R  LP++ GGSNS++HAL+A  +D     F
Sbjct: 64  LLGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPF 123

Query: 148 CGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSET 207
              +  + +E  Y CCF+W+DV   VL  YLS R+D M+  GM EE+A+F+  + A  E 
Sbjct: 124 SDHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP 183

Query: 208 RVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKI 267
            +G+ KAIGV EF++Y +  +    D  + DP++  A+E+AVR IKENT+QLTK Q+ KI
Sbjct: 184 -LGIWKAIGVQEFDDYLKM-YKWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKI 243

Query: 268 GRLRRAGWDLKRVDATA----AVVAVLASE-VAEKRSEIWETQVVEPSVKIVKGFIQE 321
            +LR AGWD+K+VDATA    A+ A    E VAE + +IW  +V+EP VKIV+  + +
Sbjct: 244 NKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

BLAST of CSPI06G03050 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 275.4 bits (703), Expect = 5.8e-74
Identity = 141/304 (46.38%), Postives = 199/304 (65.46%), Query Frame = 0

Query: 17  PRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKL 76
           P   + +  +    +++++GATGSGKS LSIDLA RF  EI+NSDK+Q Y GL +TTN++
Sbjct: 31  PMTTVCMEQSYKQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQM 90

Query: 77  PLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALV 136
            +  R  VPHHLLG+  P   E++ + FRS AS  IS + +RGNLP++ GGSNSF+HAL+
Sbjct: 91  SILERCGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALL 150

Query: 137 AADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAK 196
              FD     F  S   +S+   Y CCFLWVDVS  VL  YLSKRVD+M++ GM EELA 
Sbjct: 151 VDRFDPKTYPF-SSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAG 210

Query: 197 FYDPEMADSETRV-GLRKAIGVPEFEEYFRRHHPKGRD-YREGDPLQASAFEEAVRKIKE 256
           FYDP  + S  R  G+ K IG+PEF+ YF  + P+ +    E D  +  A++EAV++IKE
Sbjct: 211 FYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKE 270

Query: 257 NTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIV 316
           NTW+L K+Q+ +I +L+ +GWD++R+DAT +              EIW+  V++ S+K+V
Sbjct: 271 NTWRLAKKQIERIMKLKSSGWDIQRLDATPS--------FGRSSREIWDNTVLDESIKVV 325

Query: 317 KGFI 319
           K F+
Sbjct: 331 KRFL 325

BLAST of CSPI06G03050 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 211.5 bits (537), Expect = 1.0e-54
Identity = 128/296 (43.24%), Postives = 172/296 (58.11%), Query Frame = 0

Query: 27  PSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPH 86
           P + ++ ++GATGSGKS L+IDLA RF  EIINSDK+QLY+GLD+ TNK+     R VPH
Sbjct: 32  PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPH 91

Query: 87  HLLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDV 146
           HLLG FD  AG ++   +   AS  IS + +   LP+V GGSNS++ ALV          
Sbjct: 92  HLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALV---------- 151

Query: 147 FCGSRNRVSTEFL---YRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMA 206
                   S+ FL   Y CCF+WVDVS PVL  ++SKRVD M++ G++EE+ + ++P+  
Sbjct: 152 ------NHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPK-- 211

Query: 207 DSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQ 266
            +   VG+R+AIGVPE  EY R      R        ++   + AV+ IK+NT  L  RQ
Sbjct: 212 -ANYSVGIRRAIGVPELHEYLRNESLVDR------ATKSKMLDVAVKNIKKNTEILACRQ 271

Query: 267 LWKIGRLRRAGW--DLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIVKGF 318
           L KI RL +  W   + RVDAT     V      E++ E WE  V  PS +IV  F
Sbjct: 272 LKKIQRLHKK-WKMSMHRVDAT----EVFLKRNVEEQDEAWENLVARPSERIVDKF 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5GHF71.7e-8658.14Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID32.3e-8352.38Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9C6L11.3e-7347.92Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9SB602.8e-7348.99Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 ... [more]
Q9LJL48.1e-7346.38Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A0A0K9433.7e-17799.69Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G030440 PE=4 SV=1[more]
A0A5D3CF132.1e-17296.56Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BD732.1e-17296.56adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103488599 PE... [more]
A0A6J1FLX43.1e-14483.03adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111445... [more]
A0A6J1HF401.6e-14380.54adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463... [more]
Match NameE-valueIdentityDescription
XP_004150914.17.6e-17799.69adenylate isopentenyltransferase [Cucumis sativus] >KGN45943.1 hypothetical prot... [more]
XP_008445640.14.3e-17296.56PREDICTED: adenylate isopentenyltransferase-like [Cucumis melo] >KAA0049650.1 ad... [more]
XP_038886381.19.4e-15188.93adenylate isopentenyltransferase 1, chloroplastic-like [Benincasa hispida][more]
XP_022939170.16.5e-14483.03adenylate isopentenyltransferase-like [Cucurbita moschata][more]
XP_022963367.13.2e-14380.54adenylate isopentenyltransferase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G68460.11.6e-8452.38isopentenyltransferase 1 [more]
AT1G25410.18.9e-7547.92isopentenyltransferase 6 [more]
AT4G24650.12.0e-7448.99isopentenyltransferase 4 [more]
AT3G19160.15.8e-7446.38ATP/ADP isopentenyltransferases [more]
AT3G23630.11.0e-5443.24isopentenyltransferase 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01715IPPTcoord: 64..138
e-value: 1.5E-15
score: 57.6
coord: 157..264
e-value: 2.8E-13
score: 50.2
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 171..277
e-value: 5.9E-120
score: 401.5
NoneNo IPR availablePANTHERPTHR11088:SF65ADENYLATE ISOPENTENYLTRANSFERASE 4-RELATEDcoord: 24..318
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 30..318
e-value: 5.9E-120
score: 401.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 31..205
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 24..318

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G03050.1CSPI06G03050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0052381 tRNA dimethylallyltransferase activity