CSPI06G02230 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G02230
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionE3 ubiquitin-protein ligase SHPRH isoform X1
LocationChr6: 1650641 .. 1664202 (-)
RNA-Seq ExpressionCSPI06G02230
SyntenyCSPI06G02230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAGTTGTATTCGTTGGCTTCCTGCATTTCAAGTAACCCTGCAGTTTACCCCCAATGTATTTTCGCTCTAATTTCACTTAAATTTCATCACCAACACAAGAATTTTTGGCTTCTTCATCATGGTTGCGCACCTATAAGCACACTCTTTGCTTCCCAGGTTCAGTCTTTACTCTCTCGCATTTTGTTCTAAATTTTTCTTTTTCTTTTATAGCTATAGAGCTTCATACTTTTGTCAGGTGATTTTCTAGACTTTATATACTTGTTTCTCAGGTTCAGTTTTTTTATGTTTTATCTTATTTTCTCACATTTTCGTTAAATTTTGTTGAATTCTATCATATGCAACAGCACCTCTTTCTCTATATGTATGCATATTTTTCTTATGTATTTATGAACTTATATGTATGTATATTGGATTTTACCTGTGTGTAACGTAGTAATTAGTTGCTTTATATGCCGAAAAGAGCTTAACTCAACTAGCAGCATGTCTTGAGGATCAAGAATTCTGTGGTTCAAATCCTACCCCATTTGTACTCAAAAGAAAGTAGTGGCTTTATATTTCTTTTCATTTAGATATTCATTTGAGTTAATTTTTTGTTGTCTGCTTACTACTACTATAGTTTACCTTTCCATTTCAATTACATTTTAATTGTTTGAGCTTGCTCTATTTTTTGTTAAAATTGTTATAGAATGGGTAGAAGGAAGCAAAAGCAACCTAATCGCTCTGGAGGAATAAGATTTGAAGACCGTGGGGATCCCAAGACACAACTTGATATTAACGAGGTTGTGGAAACTGCTGTGGAATCATCAGACATCAAACTTGACGGGGTTAACGAGCCCATTTTTGTTGAAGTTGATCGAAGTGGATGGTATTCCAATGAGCACTATGATATTTCTGAAGTTTTTCTAGCAGATGTAAGGTTAGAACACCCTTTTGTTGGTTTTTGCTTGGATAAAAGTGTTCGTGAGAACTCGAGATACTCGTTAAGGTTTCGGTTGTGCAATGTGAATGGATCTCTCCTTGATCGTATTAAGTTTGGTCATTGGCCTGTTTTATCTTCAAATGATACATTTTTGGAATTCATTGAGAGAGACATGGAGGAAGATGTGAAAGCTTGCTCGGTGGTGTTATCGGGTAATTTAGATGGACCTGATGAGGCCATTTCTGGCCTTGTTCATCTAGCAAATCTGAAGCTTATGACGTTGAGGCCAGTTGATGGCGTTCCTTTTTCCCAAAATATGGGATCTCTGAGGTTGAGGGTGGAAATACTATCGAGTGCTTTTGATGCTTGTGAGTCAATATTTGATAATGGCAGGCAGTTGTGGAAAAAGAGTATGATGAATACCATCACTTGGTTGCGCCCTGAAGTAGTATTATCTGAGGTCAAGTATGGAGTTGTTAAATCGTCTAATATGGACACTCATTTGCATCATGAGGCGGGGGATGACACGTCCAACTCTAGAAAACATGCAAATTTTGATACTATTGGCTTTTATGATGCCATTAAGCCATCCAAGTGAGTATAGTTAGTGCTCATTTACTCATGTATCTAGTTTTAACTTACAGAACGTATTTGTTTTTCTGCCTTTCCCATTTTTTGATTGCTAGTTCAATGTAGAGATGAACCCATGCTTGATGAAGATATTCCCAATTTGCTTCCGAAACTAAGACCTTATCAGCGTCGTGCTGCCTACTGGATGATACAGCGGGAGAAAGGATTTTCTGGAAATTCAGGTTTAGGGGAAAATGTGCAGCTTATATCTCCCTTGTGTATGGCCCTGAAATGTCTTGACACTTGTTTGAGAGTGTTCTACAATCCTTTCAGGTACCACCATCTTTACTTTTGAGTGTGGTTTTGTGGCATGAATAAAGTTGAAAATATGTGTTTCTCGATGTAGTTAGGAAAACCTACTTTTCCTCTTGTATGCTTACTTCTGTTTATCTACAGTGGAAATCTTACTTTGAATGCAGAGCAAGTTTTGCCACATGTTTACGGGGGCATTCTAGCTGGTAATTCCCACTAATGAAAATTCATTACAGTTTCCTGAACTAGGCATTTCATTTATTATGAGCATGACCTCTGATGCAACTAGCTATTCTTATGGTCGTTATTTTGTCTGAATGATTCGATTTTTGCAGATGAGATGGGTCTAGGAAAAACTATTGAGCTACTGGCATGCATACTTTCTCATCAGATGTCAGTGTTTGAAGGCCGAAAAGGGTTTGATGTTGAAGTTCAACAGCTTGTTGAAGATCAGAGAACCGAATTTAAAAGATTGAAAAGAGAGCGTATTGAGTGTCTGTGCGGAGCTGTTAGTGAAAATTACAGGTACAAAGGGTTATGGGTGCAATGTGATATTTGTGATGCATGGCAACATGCTGATTGCGTTGGTTATTCACCTAAAGGAAGAATTGTCAAATCCGTTGACACTGAGGATGGGAACAGTAGAAGGGAAAAGAGAAATAATAGGAATACATTAAATGTAATTGTTAGAGCTGAGGAACATGTTTGTACACCTTGTTTAGAGCTGATGCAGGCTACTGATTCCCCAATGGCTACAGGTGCAACTCTAATTGTCTGTCCTGCTCCCATATTATTCCAGTGGCAAGCTGAGATTTTACGGTATACTCTCAGTTGACCATATTTCTACTTATATCATTCATGAGAGTGCCTTTGCATTTGATTTTTTGTACACCTGCAACTTTACCACCTCTATTAACTTGGAAATGTTGCTAAGTCACTTGTGACTACTGACTATATCAGGTTTGATAAGCGGCAGCCAAATTTACAATTTTTCTTTCAATAAGACATTGGATTACTTAATAAATAATGGTAAAGGGAATAAAAGGTTGCATGGTGGATCTCCCTATTCTCCTAAGAACAGCAGTCAAGAATTACAAATATACTTTTGCTGTCTAATATCATGAAGCTTTCACTTGATCAGATGCTTTGCATCTGAGCATGATGGTTCCTATGATCTCTAGCTTAGGACATCATACATCAATTCACTTATGTGATATTAGCTTAGTAGAAGGAAATATGTGAGGTCTTCTTTGCTGTGACCCAATTTTTTTTATGCTAAAGTGCCAATCAATAGTTCTGGTATGGATAGGGAGATTTTTATGAACCCCTGGAAAGAAATCACATTGGGTTGGAATTGGTTTAGTGATGAAAATGAAGGTGTCGTGATGAGCTGAGTTAGAAACTGTGGTTTCATTGACGTTACTCCATTTCAATTTCATTTTCTTTGTAGATTAGGCTGCACCTGTTTTTTTTCACGTGCATGCATATCATCTTTGATCAGATACTTTTGTGAAAAGACTTGACACTAACTCACTTAAAAGTTTTTGCATTTGGTAGTGCAAGCTGACTTTTCAGTTATTATTTATTATCCCAGTCATACTCATCCAGGTTCAATAAAACTACTTGTCTACGAGGGAGTGAGGGATACTTCTCTTTTAGGCACATTGTCTGCGCAGATTAACGATCTTATCAACTCTGATATAGTCTTAACATCATATGATGTGCTTAAAGAAGACCTATCACATGATTCTGATAGGCATGAAGGGGATCGTCGCTTCATGAGATTCCAGAAGAGGTCTGTTCTTACCTTCTTGAGAAATGTTCCTTACATTAATTTAATCACTTATTTCTTACATCGTAACTAGTTAGTGTAATATTTTAGATTAAGGCCGTACTCTCTTGTTTTAACCAATTTGCTTCTAGAGATAATATCCTTCTACACTAGAGAATCTTTTTTTTTATTGACATATGAAGTCAGGGGAGAACCCCAAACACCATAAGGTGGCTACATTAGAGAACGCCAATTACTGACTAAAGAGGATAAGCTGTAATGAGTAAAAGAGTGCTTTGTTTTGCACCAAGAAAAAGCCGTAAACAAGAGTAATTCCATAAAACAATAGAGGAGAAAAAAGAATCTTTATTCCCTTCATATCTGTTTTACTACATGCGAACAATTTGACTGCAGAGTTGTAGTGGTATGCTTGTGTTTCTACTTTCAAGTAATTTTAAGTTTCTAACAAGATCGGTCATATTTGATTTGTTGTCAGTCACAGGGATTTGGGGGAAATGTTGACATGTTTTCTTCAGTGTAGTTCTCTTTCAAAACTGAAAATTTTGATAATTTATCCGGACTGTTCATTTTAGGTACCCTGTTATCCCTACTCCTCTCACTAGGATATTTTGGTGGAGGATTTGTTTGGATGAGGGACAAATGGTAGAGAGCAATGCCACTGCTGCTACTGAAATGGCTTCACGACTTTATGCTAGTCACCGCTGGTGCATCACAGGGACTCCCATACAAAGGAAACTTGAAGATTTATATGGATTACTGCGATTTGTGAAAGCCAGTCCATTTAATGTTCATAGATGGTGGGTTGAAGTTATAAGAGATCCATATGAGGTATGTGGCTTTCAAAGATTTGTTCAATCATGGTTATGGAGAAAGGGAAAGGAAAGAGAGAGAGAAAATGCCTCGTAAAACCTTTGTCGGCTTTTGTTATCATAGCAGCTCAGCCATTGTTACTATTTTTTATCCGTTATCTTTTCTTACTTCTTCTTTAGGTATTATTCTGGGATTGCTTCATAGCTGTCATTGATCCATGCTATATTTTATTTTGACTTTCCTGACTCCTTTTTTATTTTACCAACAATTTGCAGAGGAGGGATCCTGGTGCTATGGAATTCACACACAAATTCTTTAAGCAAATAATGTGGCGTTCTTTAAAAATACATGTTACAGATGAATTGCAGCTACCTCCCCAGGAGGAGCAAGTCACTTGGCTAAAATTTTCACCAATTGAAGAGCATTTTTACCAGAGGCAGCATGAAACTTGTGTGAGTTATGCCCGTGAAGTTATTCAAGGTTTGAAAGATGATTTTGTCAAAAGGAAAGTCCCAGGTTGTTAGTGATTGATTGTGACTTACATTCAAGAATCATATTTCTTTGTTGATATATATATATTGACATTATAAGGGAGAAGGCTGCTGTTAATATAACTTACATTGTTTTACTGCTTTCAGATTGTGTCACTTCTGATATTCCATCCGATCTTCTTATCACCCATGCCGATGCTGGAAAACTGCTGAGCACACTTTTGAAACTTCGCCAAGCTTGCTGTCACCCGCAAGTAGGAAGTTCGGGACTCCGCTCATTGCAACAATCCCCAATGACAATGGAGGAAATATTGATGGTATTCCTCAAGTAAAGATCTGTGAGCTTTCATGAAATCTGTTGTGAGACTTTAGTCTAAGTGGTCTTATTTTCAGGTCCTTGTTAGTAAGACCAAGATAGAAGGAGAGGAAGCTCTGAGGAGGTCAGTTGTTGCTTTAAATGCACTTGCTGGAATAGCTATTATTGAGAAGAAATTTTCTGAAGCCTTCTCACTATATAAAGAAGCTCTAGAATTCGCAGAGGAGAACAATGAAGATTTTCGATTAGATCCTCTATTGAGTATTCACATCCATCACAATCTTGCTGAGATACTTCCATTAGCGGTGAACCAGTCACAATCTCCTTTAAAAGATCAACTTTGTCCAAGAACTTGCGAAGTGAAGGCATCCAGGATGGATGACTCTGAAAAGTATGATGATCATGTTCATATCATGAAGAAACAAAAAGTTAGTGAGACCCTGTATGCTACTTGCAGTGAAGATAATACAGGGAAGATGATAGACCATCCTCTTCAGCTGAAAGGAAAAGATACAAACGCCAAAAAGGAAGAAAACTATGAGCCCCATCGATCAAGTGGTTACTTTGATGAAATATCTGTAAGAAAAGTATGTGAGGTTATGAGGCAGAAATATCTAGCTGTTTTTTCCTCAAAGCTATCTATTGCCCAGCAAGAGTTCACAAAGTCCTACATGCAGGTATGTTTCTATATCCTGCTGTCTTCCTCTAGGTGGTTACAATGTGTATAGTCATTCACTATATAACCTGTGTAAGATCCTTTTAACAATAAAGATTGATGGTATGGTTACCACTATGTTCTACTATTCAGTTGCTTATTGACCCCCTTTATTCTTTGGACGCTTAAATCGTAGTTTATTTCTTTATGATGGATAGTTGGGGTGCAAAGTTCACCACTACTAACTTATATGCCATACTTGTTCAACATGAAATGAAGGTTGGAAGTGAACTCAAGGACAGGGAAAATTTTAACGACGTTTGGTGGTTGGAAGCAGTTCATCATGCTGAACAGAATAAGGACTTCTCTCATGAGTTGATAAGAAAGATTGAAGAGGCTGTATCTGGAAACCTAAACAACTCAAAATCTAGAGTTGGATCTCGGTATGTGGTGTCGAACTCTCTTCATTACATCATTACTCTTTTCCTCTCAGTTCCTCAAATCATGCTTTATGGCATGTATATGGCATGAATATTAACTGATCTTTTAGCTTGACAACAGTTTCCGCAGCATTAGTGCTTTGAAGTATCATGTTCAGAGTGGTTTGGACCTTTTGGAGGCTTCTAGGAAAGTGGTGCTTGATCGACTTTTAGAAATTGATCAGACCATGAAAAGTCCAAAAGAAGAAGATATTGAACGTGTTAGGTACTGTCGAATTTGTCAGGCAGACAGTAATGGACCACCTTGTGTTCTTTGTGAACTAGATGAACTATTTCAGGTTCTTCCTGATTAGTCCAATTACCTTGATGCTTCCTCTTTGACTTGCATTACGAATATAGGCTCTCTTCTCTTTCTAGGAATATGAGGCAAGACTTTTCCGGCTTAATAAAGTACAAGGAGGAATGGTTACTTCTGTTGAAGAGGCTGTTGAAGCTCAAAAGAAGAAGTCAGCACTCAATCGTTTCTATTGGAGTCTATTGCAGCAAAATAAGAACTCAAGTTCATCTAAAGTTGGGCATGAAGAACCTAACAAAAGAGATGCTGGAGAAAAAGTTATGGTTAGTTTTCCTTCTGGCTTTACTTTTTTCTTATGACGAGTGCCTCATATCAGTCACACGTGGCTTATAATAGGATAATCTATAGTATCTTGTGATAGAAATTGTGAGCAGTTTGATTTCTTGATATTTTTTTTAAATATTACTTAACAGTGGTTTCCCCTCCATTTCAATCACTAGACCATAACAGGTATGATACACGTGAAATTAGTAGTTTTACTTTAGTTTTTAAAAAAAGTTAAAAAAAATTCTATTTATTGAAATAGTGTTTTACATTGTTTGACTTGTATAGTTGTAACCATTTGAAGAGAAATATAAAAAAAAAATTTATGAAGTTTTAAAGGGATGGAAAATAATCTGATTGTTTGTTTAAATATATGTCCACATAATCAAGGTAAGTTCTCTCTCTCTCTTCTTTCACTTCTCTCCTCCTTTTCCCCTCCCCGACTGTCCCTCCTTCCAGTGGCAACATGGAAGTGAAGAGCTGTAAAATTGTCTGGTCCTTCTACTGCATTTGGTTCGACAAGGGACATTTCAACATTGAAGATGTTGATTTTCGAAGAATCCTACAAGTTTCAAAGAACCAAACTAAGATTGTTTATTGCCGTTATATCAGATTTGGCCAATACGAGGAACCAATTTTACAGTAGATGCAGATGGTACTACAAGACTCACAAAGTTCAGAGCTCCTTCCAGGTGGATAGTTAGATGTGTTGCTTGAAGTTCCTCAAGGTTTCAATCCTTTGTGCACGTCTATTTAGGGGGTGAGAAACAAGGATGGGTCTCTTTCTTACAAATGCTGAGAAGTTTCTTAATAAACCACAATTATGGATTGTGGCACTCATCTCTTCAACCAGCTTCTTGTTCACATGGAAATCAGTTGAATCGGTATATTTGCCTAGGCCTAAGTTATTCAGATAAAGTAAGGCTTCAAACTCAAATTTCTTCCCTGCACAACATATAAAAAAATAGAGTCTGAAGCCAAAAAACTTGATAAGCCATTCCTCTACTCCTCCAGAAACCAAACCTAGAGCTTGCAACGACAATGGGTTCGAAAATGTGACGATGTTATTAGAGAGAATTTCAGTGATTTATGGATTGAAATTCTCTCTAATAACATGCTTAGAAACTTGATCTTTTGCAAGTTTGATGATGATATGCTTAGAAACTTAAAAAAGACGCTTGAAATCTTTTCGTGAGGTTCCGGGTAAAAGATCAATTGGGAAAAGTCAGCAATTTGTGGTATTAATATAGAGGACAGCAAGGTCTCCTCTGCGGCATCCATGTTGAATTGCAAAATAGAAAACTTCCTTTAGTGTACCTAGGTCTTCCTACCCAAAGAGTATTCACTTTTGGCAGCCTGTCTTGGACAAAGTTCAGACGATGCTGTACAAATGGAAGCGTTTTAATCTAACTTGAGGAGGTAGACTTACTCTTTGCTCTTCTGTACTCTCCAATCTTCCTACCTACTGCATGTCCACTTTCCTAATGCCAGAAAAAGTAACTAAAGTTCGTTGAAAGAACTATTGAGGAACTTCTTTTGGGAAGGAAACAATGGCAGCAAATTGAACCATCTTGTTAAAATGGGACTTAGTATCTAAAGCCCAAGAAGAAGGGGGTCTCGGCCTGGACAAACTGAAAATTAGAAATATGGCTCTCCTTGCGAGTGGGGGTGGAAATTCATTGTAGATCAGAATTCTCTTTGGTGTAAGGTGGTTTGTAGTATCCATTGCAGTAGCACATTCCAATGGCACACAAGCGGCAAAGAGACCTCCAGTTTAAGAAGCCTCTGGATAAATATCTCTAGGCAGTGGCATAAAGTTGAAGCCTTGGCAGATTTAAAGTTGGAAAGCGGGACAAAACCTTGTTTTGATTGGACCTATGGCTGGACAGAATTGCTTTGGACACCAAATTTCTGAGGCTGTACAGAATAGCTCTTCTCCCTAAGGGTTCAGCTTCTGATTACTGGGATTCTTCCACCTTATCCTGGTTTATTAATTTTAGAAGATTGTTAAAAGAGGAGGAAATCTCTGATTTTCAACTTCTCATTGATTTATTGGATAGGACAAGGTTATCGAATTTAGATGATAAAAGAAGCTGGTCTCGTGGAGTTTTTTCAATAAAGTCATTATCAAGACACCTTGCTTCTGCCTCCCCTTATGACCCAAAGCTGGAAAAATGCCTTTGGAAATCTAAAAGTTTGAGGAGAGTGAATATAACCATATGGGTCTTGCTGTTCGGGAGTCTAAACTGTGCTTCAGTTATGCAAAAGAAACTTCCCACCCATTATATTTCGCCACATATTTGTCCATTTTGTCTTAGATCTGACCAGGACCTACAACATATTTTCTTTGAATGCATTTACGCCAACAAGTGTTGGAATAAACTCTTTCTCATCTTTAATCACAGCTGGGCCCTTGATAATGTTTGCAAGAATAACGTTAGACAGCTTCCAATTGGTCCAATTATGGGGGAAATTGCATCCTTGATCTGGTGCAGTGCTGTCAAGGCAATCTTGGCAGAAATTTGGTTCGAGAGAAACCAACGGGTCTTCCACCATAAATCTTCCTCTTGACAGAACAGACATGAGTTGGCACGGCTGGATGCCTCTTCATGATGCTCTCCAAGTAAACAGTTCAATGACTAGGCATGATGAGGGCGCTGGGGGATATCAACCTAGTTGAAATGTCCCTGTGCGCCTACTGATCCTTAGGTTTATTGCTCTTTGTATATCCCTCATGTACATCGAGCTTTGTCTCTTTATTATTATTTATATATTCAATAAAGAGATCAGTTTCCTTTTTTAAAAAATTAAACATATGTCCACGTTATGATGTTTCATATCTATGGTTCAACATATCCATACATGAGATGATACACCACCTTATTGTGTAACCATTTCAACCGGAATAATTATAATGGATCAAAGGATGAGATTATTTGTTTAAACATATCTCCACTAATAGATTTGATGTTTCAAATTTATGGTCCAAATCACACCCATGCACAAAAGAGTTGTATTAAGGTAAAGGACTTGATTGTTTGTTCAAACATATATTCATCATTAGATCGTGATGTTTTAATCTAGGTCAAACTATACACAAAAAGAATACATCATTTTATGTGTAACCTCCCTGACCCAAAGAGTCGTGAGTTTGTGACGAAGTACAAAACAGGAATTACTAAAAGATAATAAGGGTGAAATGAAGATTAAAGATTCTCATCTCATAGCCCATTTTGCATATATATATATATATGGTATAATGTAGATCTTCTTTTCCTTTTTTATTAATAATCATGTTGTCTTGCAACAAACCTTATTCCAAATTTTGACTTTGTTGTTCTGACTTTTAGGTGTCTAAGCATCCTTCCGAATTGGAGGTTGTTCTTGGTGTTATCAAGAACTTCTGTAAGACGCAACTAGGAAAAGAAAGTATAGCAGCAGCCAACAAGCAGCTACACCTTCTGGAGGTATTATGTGGCCATATCTATTTTTATGAGAAAACTGCCCTTTAAATGTCCTCGTTTGCTGATGACTGATTCCTTGACACTACTTTGTAAGCTATTGGTGGAAATAATTTGCATTATCAATTATATATGTTGGAGCCCTATTTAATTTATTAGACTGGGTATTATGATATAAAAGTGAACTATAATTCTATTATTTAGGCGACTTTGAATAAATGTTCAAATCTTATACATGCAATATAATTTATTAACTGGACTTATCATTCAATTTGTTTAGCACATGCGAAAGGAGTATGGACATGCAAGGTCCTTGGCCATTGCACAAGCTCAAGTTTTGAATGCTCATGACGAAATTAAGATGGCAACCACGAGATTAAGCTTAAGGGACGATGATGATGACAGCTCAGCTTTTACTATTAGTGAGCACGAGCTACCTGCAGCTAGTGTCCAGTATTCTAGCGATAAGTTCATGTCCTTGGCTATGTTATCACGTGTAAAAGGAAAACTTCGTTATTTGAAGGTTAAAAAGTTTCCAGATTTGTTAAAATAGTTATGAGCATACCCTACGTTGGCTTCTCATACAGTTCTTCCCCCTCGTTTCATTGTCTTTCTACTGGTAGTATGTATGTCATTTTACATTGTCACTCACAACCTTTATTATTTACAGGGTTTGGTGCAATCTAAACAGACCATACCATTAGATAGTTCCAGTGATCTAGCTTTAACTCAAGAACCGGCTATCACGTCAACAGCTACGGAACAAAAGAATGAGAACACATCGAAAGCTGATGAGGAATCATGCCCTGTTTGCCAAGAAAAACTAAACAATCAAAAGATGGTTTTTCAATGTGGACATATTACATGCTGTAAATGTGAGTCTCCTGCACATAATAGAAGACAATCTATTGATGTTTTAGTTCATGATAATGGCATCATTGCAGTCTCAAATTCTGTACAACTTTTATGTTTTCTTGCACTGGATAAAAGTATGATCCAGCTGATTACTTATGCTTTGAGTGAGTTGGAGATGACTCAGGAGAGTGTCAGTTTATGAGAAACACTTGCTTTATATGCACCCTAAAACTTGTCTTAATCTTTTAACAAGTTTCCAGATCATTTTTGACTTGTGTATCCAAACACCATGGAATTTAAAAAAATATATTTTTATTGATTTAAAGAACTCTTCAAAGACTCAAAATTCATACCAGGCTCAACGTTATTGTGCCAGGAAAATCCTTCAGAAAGGCAAGGAGGATGAAACCTTATCTTATCTGTTCCTCTATGAACCTTGTTCCCTTTTGAGTCTTACTTCCTAAACCTGTCATCGACGGTTTCATACATTTTCTAAATGTTCATAACATTCTGTAATGTAAATTCTAATGGAATTTGTTTCCCGCAGGTTTGTTTGCAATGACTGAAAAAACACTGCATGGTAGTAAAATTCAAACTAAATGGGTGATGTGCCCAACCTGTCGCCAACACACAGATTTTGGGAATATTGCTTATGCTGATGATAGTAAAAATGAAACACTGGATCCTTCAACTTCGCTCGAAACTTCTAGAGAGCATGAAATGTCCATTACAGTTCAAGGTTCCTATGGAACAAAGGTTTGCAGCCCTACCTAACACCCATAGTATGCGGCGATGTCTATTCCAGTGCATGTTATTGAGTCAATTGGGTTCTTGTTTTGTTTATTTGCATTGCTTGCATGTTTTCCTCAAATGTTTCCATCCTTCATCGGCACTATTTGTTTATTGCATAAACTCGTTCATCAGCTCCAGTTTTATGCATCATTTCTTGAGTTAGGTTGTTAAAAGTTAATTGAATATAGACGTGATTAGTTGGATTTGCAAAATTTCGGGTTCAGCATGCTTGATTTTCCATTCTTGTACTGGTTGTAAACGAAGGCCTCCTTTGTAGCTTATGTCCAAGTTCTAGGGTGGTGTGGGAAAAAAAATGGAAAACCTGAGGGTAAGAGAGCCTTAACATGTTTCCTAGATATTAGTTTAGTACAGATGGCTTGTGGGATATATGATATGAGGACATTATTTCCAATGCATGCCTCATGATCGTTAGTTTGACGAGTTAATTTAGTTGTATTATAAGTGTTGTCCATCAAAAAACATGGAGAGGAAATTTCATCATGTTAGTTTTTGTATCATTGCTCCAATTGTTTTGTCATTATAAGTAATACTTGCTATCTAAAACCTTTTGTAGATTGAAGCGGTTGTAAGACGAATCTTATGGATCAAGTATACAGATTCCGAAGCGAAAGTTCTAGTTTTCTCAAGTTGGAATGATGTTCTTGACGTTTTACATTATGCATTTGTTGCCAACAACATTACCTTTATCAGGATGAAAGGAGGCAGGTGAGATGATAATTTAAATGACTCAAATTCCAGACCTGGCAGTGTAGCTACGTTTTCTGCTCTTGCACATTATACGTAATAATAATATTGATGATTGGGCTTTGCAAATGCAGAAAATCTCAAACAGCCATTAGTGAATTTAGAGGACAGAAGATCAATGCAAAAGAAAATAAAAAGAAACGACTATCGACAGACTTGCCAGAATTAAGATCTGCTCAGGTTCTATTGCTCTTGATTCAACATGGAGCTAATGGCCTAAACCTTCTGGAAGCTCAACATGTTGTCCTCGTGGAACCACTTCTCAATCCAGCTGCAGAAGCGCAAGCAATTAGCAGGGTACACCGAATTGGACAAGAAAATAAGACATTCGTACACCGATTCGTAGTATGTACAATTTCCTCTTTTTTCAGTCATTTTAGAGATTTCACCAGACCATAGATTTAACAACACTCAACAAACACCTGCTTGTATAATATTATGCATATATCAATCCTTCATCACTCAACAAACACCGGTTCATCAGGTTTAACACTTTTTGGGGTTATTATAGGTTAAAGATACAGTGGAAGAGAGCATATACAAGCTAAACAGAAGCAGAGAGAGCTCTTCTTTCATCACTGGAAATGCAAAGAATCAAGATCAGCCCTTTTTGACGCTTAAAGAAGTTGAGTCTCTGTTTGCATCCAAAGCATCACCATTGAGGGAAAATGATGACAAGGGAAGTGAAACGCTAAGGCAGTTGGCTCCTTCTATGGCTGCTACTATAGCAGCTGAGAGAAGACTCAGAGAGCACACCACATAG

mRNA sequence

GGAAGTTGTATTCGTTGGCTTCCTGCATTTCAAGTAACCCTGCAGTTTACCCCCAATGTATTTTCGCTCTAATTTCACTTAAATTTCATCACCAACACAAGAATTTTTGGCTTCTTCATCATGGTTGCGCACCTATAAGCACACTCTTTGCTTCCCAGAATGGGTAGAAGGAAGCAAAAGCAACCTAATCGCTCTGGAGGAATAAGATTTGAAGACCGTGGGGATCCCAAGACACAACTTGATATTAACGAGGTTGTGGAAACTGCTGTGGAATCATCAGACATCAAACTTGACGGGGTTAACGAGCCCATTTTTGTTGAAGTTGATCGAAGTGGATGGTATTCCAATGAGCACTATGATATTTCTGAAGTTTTTCTAGCAGATGTAAGGTTAGAACACCCTTTTGTTGGTTTTTGCTTGGATAAAAGTGTTCGTGAGAACTCGAGATACTCGTTAAGGTTTCGGTTGTGCAATGTGAATGGATCTCTCCTTGATCGTATTAAGTTTGGTCATTGGCCTGTTTTATCTTCAAATGATACATTTTTGGAATTCATTGAGAGAGACATGGAGGAAGATGTGAAAGCTTGCTCGGTGGTGTTATCGGGTAATTTAGATGGACCTGATGAGGCCATTTCTGGCCTTGTTCATCTAGCAAATCTGAAGCTTATGACGTTGAGGCCAGTTGATGGCGTTCCTTTTTCCCAAAATATGGGATCTCTGAGGTTGAGGGTGGAAATACTATCGAGTGCTTTTGATGCTTGTGAGTCAATATTTGATAATGGCAGGCAGTTGTGGAAAAAGAGTATGATGAATACCATCACTTGGTTGCGCCCTGAAGTAGTATTATCTGAGGTCAAGTATGGAGTTGTTAAATCGTCTAATATGGACACTCATTTGCATCATGAGGCGGGGGATGACACGTCCAACTCTAGAAAACATGCAAATTTTGATACTATTGGCTTTTATGATGCCATTAAGCCATCCAAAGATGAACCCATGCTTGATGAAGATATTCCCAATTTGCTTCCGAAACTAAGACCTTATCAGCGTCGTGCTGCCTACTGGATGATACAGCGGGAGAAAGGATTTTCTGGAAATTCAGGTTTAGGGGAAAATGTGCAGCTTATATCTCCCTTGTGTATGGCCCTGAAATGTCTTGACACTTGTTTGAGAGTGTTCTACAATCCTTTCAGTGGAAATCTTACTTTGAATGCAGAGCAAGTTTTGCCACATGTTTACGGGGGCATTCTAGCTGATGAGATGGGTCTAGGAAAAACTATTGAGCTACTGGCATGCATACTTTCTCATCAGATGTCAGTGTTTGAAGGCCGAAAAGGGTTTGATGTTGAAGTTCAACAGCTTGTTGAAGATCAGAGAACCGAATTTAAAAGATTGAAAAGAGAGCGTATTGAGTGTCTGTGCGGAGCTGTTAGTGAAAATTACAGGTACAAAGGGTTATGGGTGCAATGTGATATTTGTGATGCATGGCAACATGCTGATTGCGTTGGTTATTCACCTAAAGGAAGAATTGTCAAATCCGTTGACACTGAGGATGGGAACAGTAGAAGGGAAAAGAGAAATAATAGGAATACATTAAATGTAATTGTTAGAGCTGAGGAACATGTTTGTACACCTTGTTTAGAGCTGATGCAGGCTACTGATTCCCCAATGGCTACAGGTGCAACTCTAATTGTCTGTCCTGCTCCCATATTATTCCAGTGGCAAGCTGAGATTTTACGTCATACTCATCCAGGTTCAATAAAACTACTTGTCTACGAGGGAGTGAGGGATACTTCTCTTTTAGGCACATTGTCTGCGCAGATTAACGATCTTATCAACTCTGATATAGTCTTAACATCATATGATGTGCTTAAAGAAGACCTATCACATGATTCTGATAGGCATGAAGGGGATCGTCGCTTCATGAGATTCCAGAAGAGGTACCCTGTTATCCCTACTCCTCTCACTAGGATATTTTGGTGGAGGATTTGTTTGGATGAGGGACAAATGGTAGAGAGCAATGCCACTGCTGCTACTGAAATGGCTTCACGACTTTATGCTAGTCACCGCTGGTGCATCACAGGGACTCCCATACAAAGGAAACTTGAAGATTTATATGGATTACTGCGATTTGTGAAAGCCAGTCCATTTAATGTTCATAGATGGTGGGTTGAAGTTATAAGAGATCCATATGAGAGGAGGGATCCTGGTGCTATGGAATTCACACACAAATTCTTTAAGCAAATAATGTGGCGTTCTTTAAAAATACATGTTACAGATGAATTGCAGCTACCTCCCCAGGAGGAGCAAGTCACTTGGCTAAAATTTTCACCAATTGAAGAGCATTTTTACCAGAGGCAGCATGAAACTTGTGTGAGTTATGCCCGTGAAGTTATTCAAGGTTTGAAAGATGATTTTGTCAAAAGGAAAGTCCCAGATTGTGTCACTTCTGATATTCCATCCGATCTTCTTATCACCCATGCCGATGCTGGAAAACTGCTGAGCACACTTTTGAAACTTCGCCAAGCTTGCTGTCACCCGCAAGTAGGAAGTTCGGGACTCCGCTCATTGCAACAATCCCCAATGACAATGGAGGAAATATTGATGGTCCTTGTTAGTAAGACCAAGATAGAAGGAGAGGAAGCTCTGAGGAGGTCAGTTGTTGCTTTAAATGCACTTGCTGGAATAGCTATTATTGAGAAGAAATTTTCTGAAGCCTTCTCACTATATAAAGAAGCTCTAGAATTCGCAGAGGAGAACAATGAAGATTTTCGATTAGATCCTCTATTGAGTATTCACATCCATCACAATCTTGCTGAGATACTTCCATTAGCGGTGAACCAGTCACAATCTCCTTTAAAAGATCAACTTTGTCCAAGAACTTGCGAAGTGAAGGCATCCAGGATGGATGACTCTGAAAAGTATGATGATCATGTTCATATCATGAAGAAACAAAAAGTTAGTGAGACCCTGTATGCTACTTGCAGTGAAGATAATACAGGGAAGATGATAGACCATCCTCTTCAGCTGAAAGGAAAAGATACAAACGCCAAAAAGGAAGAAAACTATGAGCCCCATCGATCAAGTGGTTACTTTGATGAAATATCTGTAAGAAAAGTATGTGAGGTTATGAGGCAGAAATATCTAGCTGTTTTTTCCTCAAAGCTATCTATTGCCCAGCAAGAGTTCACAAAGTCCTACATGCAGGTTGGAAGTGAACTCAAGGACAGGGAAAATTTTAACGACGTTTGGTGGTTGGAAGCAGTTCATCATGCTGAACAGAATAAGGACTTCTCTCATGAGTTGATAAGAAAGATTGAAGAGGCTGTATCTGGAAACCTAAACAACTCAAAATCTAGAGTTGGATCTCGTTTCCGCAGCATTAGTGCTTTGAAGTATCATGTTCAGAGTGGTTTGGACCTTTTGGAGGCTTCTAGGAAAGTGGTGCTTGATCGACTTTTAGAAATTGATCAGACCATGAAAAGTCCAAAAGAAGAAGATATTGAACGTGTTAGGTACTGTCGAATTTGTCAGGCAGACAGTAATGGACCACCTTGTGTTCTTTGTGAACTAGATGAACTATTTCAGGAATATGAGGCAAGACTTTTCCGGCTTAATAAAGTACAAGGAGGAATGGTTACTTCTGTTGAAGAGGCTGTTGAAGCTCAAAAGAAGAAGTCAGCACTCAATCGTTTCTATTGGAGTCTATTGCAGCAAAATAAGAACTCAAGTTCATCTAAAGTTGGGCATGAAGAACCTAACAAAAGAGATGCTGGAGAAAAAGTTATGGTGTCTAAGCATCCTTCCGAATTGGAGGTTGTTCTTGGTGTTATCAAGAACTTCTGTAAGACGCAACTAGGAAAAGAAAGTATAGCAGCAGCCAACAAGCAGCTACACCTTCTGGAGCACATGCGAAAGGAGTATGGACATGCAAGGTCCTTGGCCATTGCACAAGCTCAAGTTTTGAATGCTCATGACGAAATTAAGATGGCAACCACGAGATTAAGCTTAAGGGACGATGATGATGACAGCTCAGCTTTTACTATTAGTGAGCACGAGCTACCTGCAGCTAGTGTCCAGTATTCTAGCGATAAGTTCATGTCCTTGGCTATGTTATCACGTGTAAAAGGAAAACTTCGTTATTTGAAGGGTTTGGTGCAATCTAAACAGACCATACCATTAGATAGTTCCAGTGATCTAGCTTTAACTCAAGAACCGGCTATCACGTCAACAGCTACGGAACAAAAGAATGAGAACACATCGAAAGCTGATGAGGAATCATGCCCTGTTTGCCAAGAAAAACTAAACAATCAAAAGATGGTTTTTCAATGTGGACATATTACATGCTGTAAATGTTTGTTTGCAATGACTGAAAAAACACTGCATGGTAGTAAAATTCAAACTAAATGGGTGATGTGCCCAACCTGTCGCCAACACACAGATTTTGGGAATATTGCTTATGCTGATGATAGTAAAAATGAAACACTGGATCCTTCAACTTCGCTCGAAACTTCTAGAGAGCATGAAATGTCCATTACAGTTCAAGGTTCCTATGGAACAAAGATTGAAGCGGTTGTAAGACGAATCTTATGGATCAAGTATACAGATTCCGAAGCGAAAGTTCTAGTTTTCTCAAGTTGGAATGATGTTCTTGACGTTTTACATTATGCATTTGTTGCCAACAACATTACCTTTATCAGGATGAAAGGAGGCAGAAAATCTCAAACAGCCATTAGTGAATTTAGAGGACAGAAGATCAATGCAAAAGAAAATAAAAAGAAACGACTATCGACAGACTTGCCAGAATTAAGATCTGCTCAGGTTCTATTGCTCTTGATTCAACATGGAGCTAATGGCCTAAACCTTCTGGAAGCTCAACATGTTGTCCTCGTGGAACCACTTCTCAATCCAGCTGCAGAAGCGCAAGCAATTAGCAGGGTACACCGAATTGGACAAGAAAATAAGACATTCGTACACCGATTCGTAGTTAAAGATACAGTGGAAGAGAGCATATACAAGCTAAACAGAAGCAGAGAGAGCTCTTCTTTCATCACTGGAAATGCAAAGAATCAAGATCAGCCCTTTTTGACGCTTAAAGAAGTTGAGTCTCTGTTTGCATCCAAAGCATCACCATTGAGGGAAAATGATGACAAGGGAAGTGAAACGCTAAGGCAGTTGGCTCCTTCTATGGCTGCTACTATAGCAGCTGAGAGAAGACTCAGAGAGCACACCACATAG

Coding sequence (CDS)

ATGGGTAGAAGGAAGCAAAAGCAACCTAATCGCTCTGGAGGAATAAGATTTGAAGACCGTGGGGATCCCAAGACACAACTTGATATTAACGAGGTTGTGGAAACTGCTGTGGAATCATCAGACATCAAACTTGACGGGGTTAACGAGCCCATTTTTGTTGAAGTTGATCGAAGTGGATGGTATTCCAATGAGCACTATGATATTTCTGAAGTTTTTCTAGCAGATGTAAGGTTAGAACACCCTTTTGTTGGTTTTTGCTTGGATAAAAGTGTTCGTGAGAACTCGAGATACTCGTTAAGGTTTCGGTTGTGCAATGTGAATGGATCTCTCCTTGATCGTATTAAGTTTGGTCATTGGCCTGTTTTATCTTCAAATGATACATTTTTGGAATTCATTGAGAGAGACATGGAGGAAGATGTGAAAGCTTGCTCGGTGGTGTTATCGGGTAATTTAGATGGACCTGATGAGGCCATTTCTGGCCTTGTTCATCTAGCAAATCTGAAGCTTATGACGTTGAGGCCAGTTGATGGCGTTCCTTTTTCCCAAAATATGGGATCTCTGAGGTTGAGGGTGGAAATACTATCGAGTGCTTTTGATGCTTGTGAGTCAATATTTGATAATGGCAGGCAGTTGTGGAAAAAGAGTATGATGAATACCATCACTTGGTTGCGCCCTGAAGTAGTATTATCTGAGGTCAAGTATGGAGTTGTTAAATCGTCTAATATGGACACTCATTTGCATCATGAGGCGGGGGATGACACGTCCAACTCTAGAAAACATGCAAATTTTGATACTATTGGCTTTTATGATGCCATTAAGCCATCCAAAGATGAACCCATGCTTGATGAAGATATTCCCAATTTGCTTCCGAAACTAAGACCTTATCAGCGTCGTGCTGCCTACTGGATGATACAGCGGGAGAAAGGATTTTCTGGAAATTCAGGTTTAGGGGAAAATGTGCAGCTTATATCTCCCTTGTGTATGGCCCTGAAATGTCTTGACACTTGTTTGAGAGTGTTCTACAATCCTTTCAGTGGAAATCTTACTTTGAATGCAGAGCAAGTTTTGCCACATGTTTACGGGGGCATTCTAGCTGATGAGATGGGTCTAGGAAAAACTATTGAGCTACTGGCATGCATACTTTCTCATCAGATGTCAGTGTTTGAAGGCCGAAAAGGGTTTGATGTTGAAGTTCAACAGCTTGTTGAAGATCAGAGAACCGAATTTAAAAGATTGAAAAGAGAGCGTATTGAGTGTCTGTGCGGAGCTGTTAGTGAAAATTACAGGTACAAAGGGTTATGGGTGCAATGTGATATTTGTGATGCATGGCAACATGCTGATTGCGTTGGTTATTCACCTAAAGGAAGAATTGTCAAATCCGTTGACACTGAGGATGGGAACAGTAGAAGGGAAAAGAGAAATAATAGGAATACATTAAATGTAATTGTTAGAGCTGAGGAACATGTTTGTACACCTTGTTTAGAGCTGATGCAGGCTACTGATTCCCCAATGGCTACAGGTGCAACTCTAATTGTCTGTCCTGCTCCCATATTATTCCAGTGGCAAGCTGAGATTTTACGTCATACTCATCCAGGTTCAATAAAACTACTTGTCTACGAGGGAGTGAGGGATACTTCTCTTTTAGGCACATTGTCTGCGCAGATTAACGATCTTATCAACTCTGATATAGTCTTAACATCATATGATGTGCTTAAAGAAGACCTATCACATGATTCTGATAGGCATGAAGGGGATCGTCGCTTCATGAGATTCCAGAAGAGGTACCCTGTTATCCCTACTCCTCTCACTAGGATATTTTGGTGGAGGATTTGTTTGGATGAGGGACAAATGGTAGAGAGCAATGCCACTGCTGCTACTGAAATGGCTTCACGACTTTATGCTAGTCACCGCTGGTGCATCACAGGGACTCCCATACAAAGGAAACTTGAAGATTTATATGGATTACTGCGATTTGTGAAAGCCAGTCCATTTAATGTTCATAGATGGTGGGTTGAAGTTATAAGAGATCCATATGAGAGGAGGGATCCTGGTGCTATGGAATTCACACACAAATTCTTTAAGCAAATAATGTGGCGTTCTTTAAAAATACATGTTACAGATGAATTGCAGCTACCTCCCCAGGAGGAGCAAGTCACTTGGCTAAAATTTTCACCAATTGAAGAGCATTTTTACCAGAGGCAGCATGAAACTTGTGTGAGTTATGCCCGTGAAGTTATTCAAGGTTTGAAAGATGATTTTGTCAAAAGGAAAGTCCCAGATTGTGTCACTTCTGATATTCCATCCGATCTTCTTATCACCCATGCCGATGCTGGAAAACTGCTGAGCACACTTTTGAAACTTCGCCAAGCTTGCTGTCACCCGCAAGTAGGAAGTTCGGGACTCCGCTCATTGCAACAATCCCCAATGACAATGGAGGAAATATTGATGGTCCTTGTTAGTAAGACCAAGATAGAAGGAGAGGAAGCTCTGAGGAGGTCAGTTGTTGCTTTAAATGCACTTGCTGGAATAGCTATTATTGAGAAGAAATTTTCTGAAGCCTTCTCACTATATAAAGAAGCTCTAGAATTCGCAGAGGAGAACAATGAAGATTTTCGATTAGATCCTCTATTGAGTATTCACATCCATCACAATCTTGCTGAGATACTTCCATTAGCGGTGAACCAGTCACAATCTCCTTTAAAAGATCAACTTTGTCCAAGAACTTGCGAAGTGAAGGCATCCAGGATGGATGACTCTGAAAAGTATGATGATCATGTTCATATCATGAAGAAACAAAAAGTTAGTGAGACCCTGTATGCTACTTGCAGTGAAGATAATACAGGGAAGATGATAGACCATCCTCTTCAGCTGAAAGGAAAAGATACAAACGCCAAAAAGGAAGAAAACTATGAGCCCCATCGATCAAGTGGTTACTTTGATGAAATATCTGTAAGAAAAGTATGTGAGGTTATGAGGCAGAAATATCTAGCTGTTTTTTCCTCAAAGCTATCTATTGCCCAGCAAGAGTTCACAAAGTCCTACATGCAGGTTGGAAGTGAACTCAAGGACAGGGAAAATTTTAACGACGTTTGGTGGTTGGAAGCAGTTCATCATGCTGAACAGAATAAGGACTTCTCTCATGAGTTGATAAGAAAGATTGAAGAGGCTGTATCTGGAAACCTAAACAACTCAAAATCTAGAGTTGGATCTCGTTTCCGCAGCATTAGTGCTTTGAAGTATCATGTTCAGAGTGGTTTGGACCTTTTGGAGGCTTCTAGGAAAGTGGTGCTTGATCGACTTTTAGAAATTGATCAGACCATGAAAAGTCCAAAAGAAGAAGATATTGAACGTGTTAGGTACTGTCGAATTTGTCAGGCAGACAGTAATGGACCACCTTGTGTTCTTTGTGAACTAGATGAACTATTTCAGGAATATGAGGCAAGACTTTTCCGGCTTAATAAAGTACAAGGAGGAATGGTTACTTCTGTTGAAGAGGCTGTTGAAGCTCAAAAGAAGAAGTCAGCACTCAATCGTTTCTATTGGAGTCTATTGCAGCAAAATAAGAACTCAAGTTCATCTAAAGTTGGGCATGAAGAACCTAACAAAAGAGATGCTGGAGAAAAAGTTATGGTGTCTAAGCATCCTTCCGAATTGGAGGTTGTTCTTGGTGTTATCAAGAACTTCTGTAAGACGCAACTAGGAAAAGAAAGTATAGCAGCAGCCAACAAGCAGCTACACCTTCTGGAGCACATGCGAAAGGAGTATGGACATGCAAGGTCCTTGGCCATTGCACAAGCTCAAGTTTTGAATGCTCATGACGAAATTAAGATGGCAACCACGAGATTAAGCTTAAGGGACGATGATGATGACAGCTCAGCTTTTACTATTAGTGAGCACGAGCTACCTGCAGCTAGTGTCCAGTATTCTAGCGATAAGTTCATGTCCTTGGCTATGTTATCACGTGTAAAAGGAAAACTTCGTTATTTGAAGGGTTTGGTGCAATCTAAACAGACCATACCATTAGATAGTTCCAGTGATCTAGCTTTAACTCAAGAACCGGCTATCACGTCAACAGCTACGGAACAAAAGAATGAGAACACATCGAAAGCTGATGAGGAATCATGCCCTGTTTGCCAAGAAAAACTAAACAATCAAAAGATGGTTTTTCAATGTGGACATATTACATGCTGTAAATGTTTGTTTGCAATGACTGAAAAAACACTGCATGGTAGTAAAATTCAAACTAAATGGGTGATGTGCCCAACCTGTCGCCAACACACAGATTTTGGGAATATTGCTTATGCTGATGATAGTAAAAATGAAACACTGGATCCTTCAACTTCGCTCGAAACTTCTAGAGAGCATGAAATGTCCATTACAGTTCAAGGTTCCTATGGAACAAAGATTGAAGCGGTTGTAAGACGAATCTTATGGATCAAGTATACAGATTCCGAAGCGAAAGTTCTAGTTTTCTCAAGTTGGAATGATGTTCTTGACGTTTTACATTATGCATTTGTTGCCAACAACATTACCTTTATCAGGATGAAAGGAGGCAGAAAATCTCAAACAGCCATTAGTGAATTTAGAGGACAGAAGATCAATGCAAAAGAAAATAAAAAGAAACGACTATCGACAGACTTGCCAGAATTAAGATCTGCTCAGGTTCTATTGCTCTTGATTCAACATGGAGCTAATGGCCTAAACCTTCTGGAAGCTCAACATGTTGTCCTCGTGGAACCACTTCTCAATCCAGCTGCAGAAGCGCAAGCAATTAGCAGGGTACACCGAATTGGACAAGAAAATAAGACATTCGTACACCGATTCGTAGTTAAAGATACAGTGGAAGAGAGCATATACAAGCTAAACAGAAGCAGAGAGAGCTCTTCTTTCATCACTGGAAATGCAAAGAATCAAGATCAGCCCTTTTTGACGCTTAAAGAAGTTGAGTCTCTGTTTGCATCCAAAGCATCACCATTGAGGGAAAATGATGACAAGGGAAGTGAAACGCTAAGGCAGTTGGCTCCTTCTATGGCTGCTACTATAGCAGCTGAGAGAAGACTCAGAGAGCACACCACATAG

Protein sequence

MGRRKQKQPNRSGGIRFEDRGDPKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGWYSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWPVLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPFSQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSSNMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAAYWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVYGGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLNVIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYEGVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWLKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALAGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPLKDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGKDTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSELKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKYHVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDELFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEPNKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLSLRDDDDDSSAFTISEHELPAASVQYSSDKFMSLAMLSRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQEKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIYKLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLRENDDKGSETLRQLAPSMAATIAAERRLREHTT*
Homology
BLAST of CSPI06G02230 vs. ExPASy Swiss-Prot
Match: Q149N8 (E3 ubiquitin-protein ligase SHPRH OS=Homo sapiens OX=9606 GN=SHPRH PE=1 SV=2)

HSP 1 Score: 528.1 bits (1359), Expect = 3.7e-148
Identity = 389/1259 (30.90%), Postives = 580/1259 (46.07%), Query Frame = 0

Query: 416  RIECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNN 475
            R EC+CG + +  R     VQC  C  WQHA CV Y  K                     
Sbjct: 658  RFECICGELDQIDRKPR--VQCLKCHLWQHAKCVNYDEKN-------------------- 717

Query: 476  RNTLNVIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIK 535
                   ++ +   C  CL  M+    P++T ATLI+ P+ I  QW  EI RH    S++
Sbjct: 718  -------LKIKPFYCPHCLVAME----PVSTRATLIISPSSICHQWVDEINRHVRSSSLR 777

Query: 536  LLVYEGVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEG--DRRFMRFQK 595
            +LVY+GV+    L     Q + L   DIV+ +YDVL+ +L++    H    D R +R QK
Sbjct: 778  VLVYQGVKKDGFL-----QPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQK 837

Query: 596  RYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLY 655
            RY  IP+PL  + WWRICLDE QMVE     A EMA RL   +RWCI+GTP+QR LEDL+
Sbjct: 838  RYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGTPVQRGLEDLF 897

Query: 656  GLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPP 715
            GL+ F+   P+ V  WWV ++  PY +++P   +  + F  +I+WRS K  V D++Q+PP
Sbjct: 898  GLVVFLGIEPYCVKHWWVRLLYRPYCKKNP---QHLYSFIAKILWRSAKKDVIDQIQIPP 957

Query: 716  QEEQVTWLKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLIT 775
            Q E++ WL FSP+E HFY RQHE C    ++V+  L      RK+ D        D    
Sbjct: 958  QTEEIHWLHFSPVERHFYHRQHEVC---CQDVVVKL------RKISDWALKLSSLD---- 1017

Query: 776  HADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRS 835
                  +L  LL+LRQACCHPQ        LQ+S MTMEE+L  L  K   E EEA R+ 
Sbjct: 1018 RRTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQL 1077

Query: 836  VVALNALAGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAV 895
            V ALN LAGI II+ +++ A  LY+E L  +EE+    + D L  +H  HNL E+L    
Sbjct: 1078 VCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELL---- 1137

Query: 896  NQSQSPLKDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDH 955
                                              I +   +  TL         G++ + 
Sbjct: 1138 ----------------------------------IARHPGIPPTL-------RDGRLEEE 1197

Query: 956  PLQLKGKDTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKS 1015
              QL                                  R+ Y++  +++++ AQQ     
Sbjct: 1198 AKQL----------------------------------REHYMSKCNTEVAEAQQAL-YP 1257

Query: 1016 YMQVGSELKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNL--NNSKSRVGSR 1075
              Q   EL+ + + N  WWL  +H A +      EL++++   ++ N      K  +  +
Sbjct: 1258 VQQTIHELQRKIHSNSPWWLNVIHRAIE-FTIDEELVQRVRNEITSNYKQQTGKLSMSEK 1317

Query: 1076 FRSISALKYHVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGP 1135
            FR    L++ + + ++ L   +K+V + +  ++     P    IE    C +  A     
Sbjct: 1318 FRDCRGLQFLLTTQMEELNKCQKLVREAVKNLE---GPPSRNVIESATVCHLRPARLPLN 1377

Query: 1136 PCVLCELDELFQEYEARLFRLNKVQGGMVTSVEEAVEAQK-----KKSALNRFYWSLLQQ 1195
             CV C+ DELF EYE++LF  N V+ G     EE +E ++     +     R  W++   
Sbjct: 1378 CCVFCKADELFTEYESKLFS-NTVK-GQTAIFEEMIEDEEGLVDDRAPTTTRGLWAI--- 1437

Query: 1196 NKNSSSSKVGHEEPNKRDAGEKVMVSKHPSELEVVLGVIKNFCKT-QLGKESIAAANKQL 1255
                                         SE E  +  I +F K+ +   E +   +  +
Sbjct: 1438 -----------------------------SETERSMKAILSFAKSHRFDVEFVDEGSTSM 1497

Query: 1256 HLLEHMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLSLRDDDDDSS----AFTISEHEL 1315
             L E  +KEY       +A    ++A DE+ MAT RL +RD  +          I  HE+
Sbjct: 1498 DLFEAWKKEYKLLHEYWMALRNRVSAVDELAMATERLRVRDPREPKPNPPVLHIIEPHEV 1557

Query: 1316 PAASVQYSSDKFMSLAMLSRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATE 1375
                ++  +DK ++ + L +  G+L YL  L +S                          
Sbjct: 1558 EQNRIKLLNDKAVATSQLQKKLGQLLYLTNLEKS-------------------------- 1617

Query: 1376 QKNENTSKADEESCPVCQEKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCP 1435
             +++ +   + E CP+C  +L  Q  V  CGH  C +C+  + E+   GS   +  + C 
Sbjct: 1618 -QDKTSGGVNPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSS--IKCA 1677

Query: 1436 TCRQHTDFGNIAYADDSKNETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKY 1495
             CRQ T    I+Y   S+                E  I V+GS+ TK+EAVVR ++ I+ 
Sbjct: 1678 ICRQTTSHKEISYVFTSE------------KANQEEDIPVKGSHSTKVEAVVRTLMKIQL 1682

Query: 1496 TDSEAKVLVFSSWNDVLDVLHYAFVANNITFIRMKGGRKSQTAISEF-RGQKINAKENKK 1555
             D  AK LVFS+W DVLD++  A   NN+ F ++   +  Q  +S F R  +IN      
Sbjct: 1738 RDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKRDPQIN------ 1682

Query: 1556 KRLSTDLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQE 1615
                          +LLL +  G+NGL ++EA HV+LVEP+LNPA E QAI RVHRIGQ 
Sbjct: 1798 --------------ILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQT 1682

Query: 1616 NKTFVHRFVVKDTVEESIYKLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPL 1660
              T VHRF++K T+EE +  + ++ E  S    +AK+ +   LT+ ++  LF  +   L
Sbjct: 1858 KPTIVHRFLIKATIEERMQAMLKTAE-RSHTNSSAKHSEASVLTVADLADLFTKETEEL 1682

BLAST of CSPI06G02230 vs. ExPASy Swiss-Prot
Match: Q7TPQ3 (E3 ubiquitin-protein ligase SHPRH OS=Mus musculus OX=10090 GN=Shprh PE=1 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.7e-145
Identity = 381/1259 (30.26%), Postives = 575/1259 (45.67%), Query Frame = 0

Query: 416  RIECLCGAVSE-NYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRN 475
            R EC+CG   +  ++ +   VQC  C  WQHA CV Y  K                    
Sbjct: 649  RFECICGEFDQIGHKPR---VQCLKCHLWQHAKCVNYEEKN------------------- 708

Query: 476  NRNTLNVIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSI 535
                    ++ +   C  CL  M+    P++T ATLI+ P+ I  QW  EI RH    S+
Sbjct: 709  --------LKVKPFYCPHCLVAME----PVSTRATLIISPSSICHQWVDEINRHVRSSSL 768

Query: 536  KLLVYEGVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEG--DRRFMRFQ 595
            ++LVY+GV+    L     Q + L   DIV+ +YDVL+ +L++ +  H    D R +R Q
Sbjct: 769  RVLVYQGVKKHGFL-----QPHFLAEQDIVIITYDVLRSELNYVNIPHSNSEDGRRLRNQ 828

Query: 596  KRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDL 655
            KRY  IP+PL  + WWRICLDE QMVE     A EMA RL   +RWCI+GTP+QR LEDL
Sbjct: 829  KRYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGTPVQRGLEDL 888

Query: 656  YGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLP 715
            +GL+ F+   P+ V  WW+ ++  PY +++P   +  + F  +IMWRS K  V D++Q+P
Sbjct: 889  FGLVVFLGIEPYCVKHWWIRLLYHPYCKKNP---QHLYSFIAKIMWRSAKKDVIDQIQIP 948

Query: 716  PQEEQVTWLKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLI 775
            PQ E++ WL FSP+E HFY RQHE C   A   ++ + D  +K    D  T         
Sbjct: 949  PQTEEMHWLHFSPVERHFYHRQHEVCCQDAIVKLRKISDWALKLSSLDRRT--------- 1008

Query: 776  THADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR 835
                   +L  LL+LRQACCHPQ        LQ+S MTMEE+L  L  K   E EEA R+
Sbjct: 1009 ----VSSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQ 1068

Query: 836  SVVALNALAGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLA 895
             V ALN LAGI II+ +++ A  LY+E L  +EE+    + D L  +H  HNL E+L   
Sbjct: 1069 LVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELL--- 1128

Query: 896  VNQSQSPLKDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMID 955
                                                                        
Sbjct: 1129 ------------------------------------------------------------ 1188

Query: 956  HPLQLKGKDTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTK 1015
                         K     P    G  +E       + +R+ Y++  +++++ AQQ   +
Sbjct: 1189 -----------GAKHPGIPPTLRDGRLEE-----EAKQLREHYMSKCNTEVAEAQQAL-Q 1248

Query: 1016 SYMQVGSELKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNL--NNSKSRVGS 1075
               Q   EL+ + + N  WWL  +H A        EL++++   +S N      K  +  
Sbjct: 1249 PVQQSIRELQRKIHSNSPWWLNVIHRA-MEFSVDEELVQRVRNEISSNYKQQTDKLSMSE 1308

Query: 1076 RFRSISALKYHVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNG 1135
            +FR    L++ + + ++ L   +K+V + + +++   K P  E IE    C +  A    
Sbjct: 1309 KFRDCRGLQFLLTTQMEELHKFQKLVREAVKKLE---KPPSREVIESATVCHLRPARLPL 1368

Query: 1136 PPCVLCELDELFQEYEARLFRLNKVQGGMVTSVEEAVEAQK-----KKSALNRFYWSLLQ 1195
              CV C+ DELF EYE++LF  N V+ G     EE +E ++     +     R  W++  
Sbjct: 1369 NCCVFCKADELFTEYESKLF-FNTVK-GQTAIFEEMIEDEEGLVDDRVPTTTRGLWAV-- 1428

Query: 1196 QNKNSSSSKVGHEEPNKRDAGEKVMVSKHPSELEVVLGVIKNFCKT-QLGKESIAAANKQ 1255
                                          SE E  +  I +F ++ +   E +   +  
Sbjct: 1429 ------------------------------SETERSMKAILSFARSHRFDVEYVDEGSVS 1488

Query: 1256 LHLLEHMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLSLRDDDDDSS----AFTISEHE 1315
            + L E  +KEY       +     ++A DE+ MAT RL +R   +          I  HE
Sbjct: 1489 MDLFEAWKKEYKLLHEYWMTLRNRVSAVDELAMATERLRVRHPKEPKPNPPVHHIIEPHE 1548

Query: 1316 LPAASVQYSSDKFMSLAMLSRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAT 1375
            +    ++  +DK ++ + L +  G+L YL  L +S                         
Sbjct: 1549 VEQNRIKLVNDKAVATSQLQKKLGQLLYLTNLEKS------------------------- 1608

Query: 1376 EQKNENTSKADEESCPVCQEKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMC 1435
              +++ +   + E CP+C  +L  Q  V  CGH  C +C   + E+   GS   +  + C
Sbjct: 1609 --QDKTSGGINPEPCPICARQLGKQWAVLTCGHCFCNECTSIIIEQYSVGSHRSS--IKC 1668

Query: 1436 PTCRQHTDFGNIAYADDSKNETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIK 1495
              CRQ T    ++Y   S+                E  I V+GS+ TK+EAVVR ++ I+
Sbjct: 1669 AICRQTTSHKEVSYVFTSE------------KANQEDDIPVKGSHSTKVEAVVRTLMKIQ 1673

Query: 1496 YTDSEAKVLVFSSWNDVLDVLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKK 1555
              D  AK LVFS+W DVLD++  A   NN+ F ++   +  Q  +S F+           
Sbjct: 1729 LRDPGAKALVFSTWQDVLDIISKALTDNNMEFTQISRIKTFQENLSAFKYD--------- 1673

Query: 1556 KRLSTDLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQE 1615
                   P +    +LLL +  G+NGL ++EA HV+LVEP+LNPA E QAI RVHRIGQ 
Sbjct: 1789 -------PHI---NILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQT 1673

Query: 1616 NKTFVHRFVVKDTVEESIYKLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPL 1660
              T VHRF++K T+EE +  + ++ E  S  + + K+ +   LT+  +  LF  +   L
Sbjct: 1849 KPTIVHRFLIKATIEERMQAMLKTAE-RSHTSSSGKHSEASVLTVAGLADLFTKENEEL 1673

BLAST of CSPI06G02230 vs. ExPASy Swiss-Prot
Match: Q9UTL9 (Uncharacterized ATP-dependent helicase C144.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC144.05 PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 4.2e-59
Identity = 327/1403 (23.31%), Postives = 558/1403 (39.77%), Query Frame = 0

Query: 286  PNLLPKLRPYQRRAAYWMIQR--EKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNP 345
            P L  +L P+Q R   WM +R  EK  + N         + PL    K L     V+ N 
Sbjct: 212  PRLSSELLPFQMRVLEWMKRREEEKFLTSND--------LPPLWYHCKSLFDDRMVYVNH 271

Query: 346  FSGNLTLNAEQVL----PHVYGGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQ 405
              G +T + E+        + GGILADEMG+GKT+E+L  +L HQ+ +            
Sbjct: 272  VYGYMTFSKEKTYLLASGDIRGGILADEMGMGKTLEVLGLVLHHQLPI------------ 331

Query: 406  QLVEDQRTEFKRLKRERIECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVK 465
                                                   + D       VG + K     
Sbjct: 332  --------------------------------------SLTDTCTFDQVVGKNVK----- 391

Query: 466  SVDTEDGNSRREKRNNRNTLNVIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILF 525
                                                          + ATLI+ P+ IL 
Sbjct: 392  ---------------------------------------------YSKATLIITPSTILD 451

Query: 526  QWQAEILRHTHPGSIKLLVYEGVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDS 585
            QW +EI    H  S+K+  Y+G+R ++  G  SA+I   ++ DIV+TSY  L+ +L +  
Sbjct: 452  QWLSEI--DLHVPSLKVFHYQGIRKSN--GLKSAKI--FLDCDIVVTSYSDLRFELLYT- 511

Query: 586  DRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWC 645
               E   R +R +KR+    +PL  + WWRIC+DE QMVE++ +   +M  R+   + W 
Sbjct: 512  ---ESHSRTLRHEKRHVSPKSPLIDVCWWRICVDEAQMVETSQSNVAQMIYRIPRVNCWT 571

Query: 646  ITGTPIQRKLEDLYGLLRFVKASPFNVHR--WWVEVIRDPYERRDPGAMEFTHKFFKQIM 705
            ++GTP++ +++DL+GLL  ++ SP  +++   W+++I     R      EF    F  ++
Sbjct: 572  VSGTPVRSEVDDLFGLLFLLRYSPMYLYKKQAWMQIIEKKRVR------EFC-DLFGSLV 631

Query: 706  WRSLKIHVTDELQLPPQEEQVTWLKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRK 765
             R  K  V +EL+LPPQ       + S +EE  YQ      +S A + +   KD    R 
Sbjct: 632  CRHSKQDVEEELKLPPQHRICMTTRLSVVEETNYQ----DLLSEAAKSLHFFKD----RN 691

Query: 766  VPDCVTSDIPSDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPM-TMEEILM 825
            +  C              D   +   L++LRQACCHPQVG     +    PM ++ ++L+
Sbjct: 692  LDLC--------------DEESMRRWLVRLRQACCHPQVGFGNKSAFGGGPMKSINDVLV 751

Query: 826  VLVSKTKIEGEEALRRSVVALNALAG-IAIIEKKFSEAFSLYKEALEFAEENNEDFRLDP 885
             ++ +T      +L R + +   + G I    K +++A +++ E     E          
Sbjct: 752  FMLEQTN-STFSSLNRKLYSDKIIVGQIYDHIKDYNKALAIWSEVRIPVE---------- 811

Query: 886  LLSIHIHHNLAEILPLAVNQSQSPLKDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVS 945
                           LAV + ++ +                  + KY+DH          
Sbjct: 812  ---------------LAVKELENVIY-----------------NSKYEDH---------- 871

Query: 946  ETLYATCSEDNTGKMIDHPLQLKGKDTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKY 1005
                        GK +  P+   G D                +F  + V  V  ++ + Y
Sbjct: 872  ------------GKNL--PINYFGLD----------------HFIHLRVWYV--LLHKIY 931

Query: 1006 LAVFSSKLSIAQQEFTKSYMQVGSELKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEE 1065
              + S+  S+  ++F   +  +  +L+ R+  +DV    + H  E ++ F  + + KI  
Sbjct: 932  FFIASAYFSLKNEKFENEFYLLAQDLR-RKIMSDVIIKTSKHLEEFSEKFIPKKLVKIPR 991

Query: 1066 AVSGNLNNSKSRVGSRFRSISALKYHVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDI 1125
                      +  G      + L   +    ++L   R    DRL+ + +     +E D 
Sbjct: 992  LQKSYAKGLITGHGI-IEDYNRLYKELNDQKEVLIKFR----DRLIHLMKLPLLDQESDP 1051

Query: 1126 ERVRYCRICQADSNGPPCVLCELDELFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKK-- 1185
                Y     A S    C+     +++++  +   R+  V G + T V    E +K K  
Sbjct: 1052 TGDEYEESLNAQSEISYCI-----DVYRQMLSD--RVAAVSGTINTFVSHETELEKYKLI 1111

Query: 1186 SALNRFYWSLLQQNKNSSSSKVGHEEPNKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQL 1245
             ++ +   SL +Q +      + + E  + +A  K    ++ S + +V  ++    ++  
Sbjct: 1112 ESIKKSEKSLDKQAEERDKKYLLYFE-EREEARPK--ADQYGSLINIVSRLLDASNRSTS 1171

Query: 1246 GKESI----------AAANKQLHLLEHMRKEYGHARSLAIAQAQVLNAHDEIKMA----- 1305
              E+           A A +Q  + + + KE    +    ++ +      EI  +     
Sbjct: 1172 SFETSKNMEEYERIDAMAKEQSRICQKLEKELSIIQLTYNSRIEYYKQLQEISDSLMPPP 1231

Query: 1306 TTRLSLRD---DDDDSSAF--------------TISEHELPAASVQYSSDKFMSLAMLSR 1365
             + +SL +   DD+    F               ISE +  A+     ++          
Sbjct: 1232 VSNISLNNYVKDDEKKQKFLNSVIIKASVILEKEISEKQDEASQTTNVAELVNQKISEMN 1291

Query: 1366 VKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKAD-EESCPVCQE 1425
            + G +  L+ L + K      S++   +    +     T        KA+  + C +C++
Sbjct: 1292 IPGHIHLLRELEEEK------SNTQRKIAHFESRRRYLTNLYEHIVLKAESHQICIICRD 1324

Query: 1426 KLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1485
             +  Q  +  CGH+ C  CL A  +   H S        CP C+   +  N  Y  +S++
Sbjct: 1352 -IIKQGFITTCGHLYCSFCLEAWLK---HSSS-------CPMCKTKLNKNNAYYIGESRD 1324

Query: 1486 ------------------ETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYT 1545
                              E LD     + S     ++ ++ S+G+KI+ + + +L++K+ 
Sbjct: 1412 IYSRQEFVTGFNKRDERLEILDDEAYRQIS-----NMELKESFGSKIDTISKHLLYLKHN 1324

Query: 1546 DSEAKVLVFSSWNDVLDVLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKR 1605
            +   KV+VFS W DVLDVLH +F AN I FIR  G  K+ T +  F+             
Sbjct: 1472 ELYPKVVVFSQWLDVLDVLHKSFEANGIVFIRFDGKSKN-TCLKRFK------------- 1324

Query: 1606 LSTDLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENK 1626
                  E RS QVL L  +  ++GL L  A HV + EPLLN   E QAISRVHRIGQ   
Sbjct: 1532 ------EERSLQVLTLHARSQSSGLTLTNATHVFMCEPLLNSGIEMQAISRVHRIGQTRP 1324

BLAST of CSPI06G02230 vs. ExPASy Swiss-Prot
Match: Q9FNI6 (DNA repair protein RAD5A OS=Arabidopsis thaliana OX=3702 GN=RAD5A PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 7.2e-43
Identity = 157/573 (27.40%), Postives = 236/573 (41.19%), Query Frame = 0

Query: 237 VKSSNMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPN-LLPKLRPY 296
           VK+ N D +     GD+        + D I     +       + + + P+ LL +LRPY
Sbjct: 302 VKNMNQDAN-----GDENEQCISDGDLDNI-----VGVGDSSGLKEMETPHTLLCELRPY 361

Query: 297 QRRAAYWMIQREKGFSGNSGLGENVQLISPLCMALKCL--DTCLRVFYNPFSGNLTLNAE 356
           Q++A +WM Q EKG    +   E   ++ P C    CL     L V+ N F+G+ T++  
Sbjct: 362 QKQALHWMTQLEKG----NCTDEAATMLHP-CWEAYCLADKRELVVYLNSFTGDATIHFP 421

Query: 357 QVLPHVYGGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLK 416
             L    GGILAD MGLGKT+  ++ +L+H                              
Sbjct: 422 STLQMARGGILADAMGLGKTVMTISLLLAH------------------------------ 481

Query: 417 RERIECLCGAVSENYRYKGLWVQCDICDAWQHAD----CVGYSPKGRIVKSVDTEDGNSR 476
                                       +W+ A     C  Y     I  SVD       
Sbjct: 482 ----------------------------SWKAASTGFLCPNYEGDKVISSSVD------- 541

Query: 477 REKRNNRNTLNVIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHT 536
                  +  +  V+A + +      L Q   S +  G  LIVCP  +L QW+ EI  H 
Sbjct: 542 -------DLTSPPVKATKFLGFDKRLLEQ--KSVLQNGGNLIVCPMTLLGQWKTEIEMHA 601

Query: 537 HPGSIKLLVYEG---VRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHD-SDRHEGD 596
            PGS+ + V+ G    +D  LL            SD+V+T+Y VL  + S + S  HEG 
Sbjct: 602 KPGSLSVYVHYGQSRPKDAKLLS----------QSDVVITTYGVLTSEFSQENSADHEG- 661

Query: 597 RRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPI 656
                           +  + W+RI LDE   ++++ +  +  A+ L A  RWC+TGTPI
Sbjct: 662 ----------------IYAVRWFRIVLDEAHTIKNSKSQISLAAAALVADRRWCLTGTPI 721

Query: 657 QRKLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHV 716
           Q  LEDLY LLRF++  P+    WW ++++ P+E  D   ++      K IM R  K   
Sbjct: 722 QNNLEDLYSLLRFLRIEPWGTWAWWNKLVQKPFEEGDERGLKLVQSILKPIMLRRTKSST 736

Query: 717 TDE----LQLPPQEEQVTWLKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDC 776
             E    L LPP + +V + + S  E  FY         + R  ++   D FV++     
Sbjct: 782 DREGRPILVLPPADARVIYCELSESERDFYD------ALFKRSKVK--FDQFVEQ----- 736

Query: 777 VTSDIPSDLLITHADAGKLLSTLLKLRQACCHP 795
                   +L  +A    +L  LL+LRQ C HP
Sbjct: 842 ------GKVLHNYA---SILELLLRLRQCCDHP 736


HSP 2 Score: 104.4 bits (259), Expect = 1.3e-20
Identity = 77/290 (26.55%), Postives = 135/290 (46.55%), Query Frame = 0

Query: 1365 ENTSKADEESCPVCQEKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCR 1424
            E   K ++  CP+C E L +  ++  C H  C +CL A    +  G        +CP CR
Sbjct: 784  EELRKGEQGECPICLEALED-AVLTPCAHRLCRECLLASWRNSTSG--------LCPVCR 843

Query: 1425 QHTDFGNIAYADDSKNETLDPSTSLETSREHEMSITVQGSY--GTKIEAVVRRILWIKYT 1484
                         SK E +   T      E    + V+ ++   +KI A++  +  ++  
Sbjct: 844  NTV----------SKQELITAPT------ESRFQVDVEKNWVESSKITALLEELEGLR-- 903

Query: 1485 DSEAKVLVFSSWNDVLDVLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKR 1544
             S +K ++FS W   LD+L      NN +F+R+ G                   + ++++
Sbjct: 904  SSGSKSILFSQWTAFLDLLQIPLSRNNFSFVRLDG----------------TLSQQQREK 963

Query: 1545 LSTDLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENK 1604
            +  +  E  S  VLL+ ++ G  G+NL  A +  +++P  NPA E QA+ R+HRIGQ  +
Sbjct: 964  VLKEFSEDGSILVLLMSLKAGGVGINLTAASNAFVMDPWWNPAVEEQAVMRIHRIGQTKE 1023

Query: 1605 TFVHRFVVKDTVEESIYKLNRSRESSSFITGNAKNQDQPFLTLKEVESLF 1653
              + RF+VK TVEE +  +   ++    I+G   +Q+     ++E++ LF
Sbjct: 1024 VKIRRFIVKGTVEERMEAVQARKQ--RMISGALTDQEVRSARIEELKMLF 1028

BLAST of CSPI06G02230 vs. ExPASy Swiss-Prot
Match: Q9FIY7 (DNA repair protein RAD5B OS=Arabidopsis thaliana OX=3702 GN=RAD5B PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 2.8e-39
Identity = 144/523 (27.53%), Postives = 210/523 (40.15%), Query Frame = 0

Query: 292  LRPYQRRAAYWMIQREKGFSGNSGLGENVQLISPLCMALK-CLDTCLRVFYNPFSGNLTL 351
            LRPYQ++A YWM + EKG      + +  + + P   A + C +    ++ N FSG  T+
Sbjct: 616  LRPYQKQALYWMSESEKGID----VEKAAETLHPCWEAYRICDERAPSIYLNIFSGEATI 675

Query: 352  NAEQVLPHVYGGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFK 411
                      GGILAD MGLGKT+  +A IL+       GR   + E             
Sbjct: 676  QFPTATQMARGGILADAMGLGKTVMTIALILAR-----PGRGNPENE------------- 735

Query: 412  RLKRERIECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRR 471
                                       D+  A  +AD                       
Sbjct: 736  ---------------------------DVLVADVNAD----------------------- 795

Query: 472  EKRNNRNTLNVIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTH 531
             KRN +                  E+  A  +  A G TLI+CP  +L QW+ E+  H+ 
Sbjct: 796  -KRNRK------------------EIHMALTTVKAKGGTLIICPMALLSQWKDELETHSK 855

Query: 532  PGSIKLLVYEGVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMR 591
            P ++ +LVY G       G  +     + + D+VLT+Y VL      D            
Sbjct: 856  PDTVSVLVYYG-------GDRTHDAKAIASHDVVLTTYGVLTSAYKQD------------ 915

Query: 592  FQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLE 651
                  +  +   RI W+RI LDE   ++S  T A +    L +  RWC+TGTP+Q KLE
Sbjct: 916  ------MANSIFHRIDWYRIVLDEAHTIKSWKTQAAKATFELSSHCRWCLTGTPLQNKLE 975

Query: 652  DLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDE-- 711
            DLY LL F+   P+    WW ++I+ PYE  DP  ++      + +M R  K     E  
Sbjct: 976  DLYSLLCFLHVEPWCNWAWWSKLIQKPYENGDPRGLKLIKAILRPLMLRRTKETRDKEGS 1000

Query: 712  --LQLPPQEEQVTWLKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDI 771
              L+LPP + QV   + S  E  FY         + R  +Q   D FV +          
Sbjct: 1036 LILELPPTDVQVIECEQSEAERDFY------TALFKRSKVQ--FDQFVAQ---------- 1000

Query: 772  PSDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPM 810
               +L  +A+   +L  LL+LRQ C HP +  S   S Q + +
Sbjct: 1096 -GKVLHNYAN---ILELLLRLRQCCNHPFLVMSRADSQQYADL 1000


HSP 2 Score: 98.6 bits (244), Expect = 7.2e-19
Identity = 83/324 (25.62%), Postives = 139/324 (42.90%), Query Frame = 0

Query: 1335 SKQTIPLDSSSDLALTQEP------AITSTATEQKNENTSKADEESCPVCQEKLNNQKMV 1394
            S+Q   LDS +   L   P      A +    E+  ++    + + CP+C E  ++  ++
Sbjct: 994  SQQYADLDSLARRFLDNNPDSVSQNAPSRAYIEEVIQDLRDGNSKECPICLESADD-PVL 1053

Query: 1395 FQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTS 1454
              C H  C +CL         G        +CP CR              + E +    S
Sbjct: 1054 TPCAHRMCRECLLTSWRSPSCG--------LCPICRTIL----------KRTELI----S 1113

Query: 1455 LETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLHYAFVAN 1514
              T     + +       +K+  +++ +  IK + S  K +VFS W   LD+L       
Sbjct: 1114 CPTDSIFRVDVVKNWKESSKVSELLKCLEKIKKSGSGEKSIVFSQWTSFLDLLEIPLRRR 1173

Query: 1515 NITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLIQHGANGLN 1574
               F+R  G                   +  ++++  +  E +   +LL+ ++ G  GLN
Sbjct: 1174 GFEFLRFDG----------------KLAQKGREKVLKEFNETKQKTILLMSLKAGGVGLN 1233

Query: 1575 LLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIYKLNRSRESS 1634
            L  A  V L++P  NPA E QAI R+HRIGQ+   FV RF+VKDTVEE + ++   ++  
Sbjct: 1234 LTAASSVFLMDPWWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQVQARKQ-- 1276

Query: 1635 SFITGNAKNQDQPFLTLKEVESLF 1653
              I G   +++     L+E++ LF
Sbjct: 1294 RMIAGALTDEEVRSARLEELKMLF 1276

BLAST of CSPI06G02230 vs. ExPASy TrEMBL
Match: A0A0A0K8S1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014630 PE=3 SV=1)

HSP 1 Score: 3355.8 bits (8700), Expect = 0.0e+00
Identity = 1688/1693 (99.70%), Postives = 1690/1693 (99.82%), Query Frame = 0

Query: 1    MGRRKQKQPNRSGGIRFEDRGDPKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 60
            MGRRKQKQPNRSGGIRFEDRGD KTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW
Sbjct: 101  MGRRKQKQPNRSGGIRFEDRGDHKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 160

Query: 61   YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120
            YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP
Sbjct: 161  YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 220

Query: 121  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 180
            VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF
Sbjct: 221  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 280

Query: 181  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 240
            SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS
Sbjct: 281  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 340

Query: 241  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 300
            NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA
Sbjct: 341  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 400

Query: 301  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 360
            YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY
Sbjct: 401  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 460

Query: 361  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 420
            GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL
Sbjct: 461  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 520

Query: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 480
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN
Sbjct: 521  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 580

Query: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE 540
            VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGS+KLLVYE
Sbjct: 581  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSMKLLVYE 640

Query: 541  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
            GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 641  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 700

Query: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK
Sbjct: 701  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 760

Query: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
            ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 761  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 820

Query: 721  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 780
            LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL
Sbjct: 821  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 880

Query: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL
Sbjct: 881  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 940

Query: 841  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 900
            AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL
Sbjct: 941  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 1000

Query: 901  KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 960
            KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK
Sbjct: 1001 KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 1060

Query: 961  DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020
            DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE
Sbjct: 1061 DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1120

Query: 1021 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080
            LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1121 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1180

Query: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1140
            HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE
Sbjct: 1181 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1240

Query: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1200
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP
Sbjct: 1241 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1300

Query: 1201 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1260
            NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS
Sbjct: 1301 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1360

Query: 1261 LAIAQAQVLNAHDEIKMATTRLSLR-DDDDDSSAFTISEHELPAASVQYSSDKFMSLAML 1320
            LAIAQAQVLNAHDEIKMATTRLSLR DDDDDSSAFTISEHELPAASVQYSSDKFMSLAML
Sbjct: 1361 LAIAQAQVLNAHDEIKMATTRLSLRGDDDDDSSAFTISEHELPAASVQYSSDKFMSLAML 1420

Query: 1321 SRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQ 1380
            SRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQ
Sbjct: 1421 SRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQ 1480

Query: 1381 EKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSK 1440
            EKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSK
Sbjct: 1481 EKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSK 1540

Query: 1441 NETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLD 1500
            NETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLD
Sbjct: 1541 NETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLD 1600

Query: 1501 VLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLL 1560
            VLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLL
Sbjct: 1601 VLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLL 1660

Query: 1561 IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIY 1620
            IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRF+VKDTVEESIY
Sbjct: 1661 IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIY 1720

Query: 1621 KLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLRENDDKGSETLRQLAPSMAA 1680
            KLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPL ENDDKGSETLRQLAPSMAA
Sbjct: 1721 KLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAA 1780

Query: 1681 TIAAERRLREHTT 1693
            TIAAERRLREHTT
Sbjct: 1781 TIAAERRLREHTT 1793

BLAST of CSPI06G02230 vs. ExPASy TrEMBL
Match: A0A1S3C3N1 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496631 PE=3 SV=1)

HSP 1 Score: 3226.0 bits (8363), Expect = 0.0e+00
Identity = 1617/1692 (95.57%), Postives = 1648/1692 (97.40%), Query Frame = 0

Query: 1    MGRRKQKQPNRSGGIRFEDRGDPKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 60
            MGRRKQKQP+ SGGIRFEDRGDPKTQLDIN V ETA E+SDIKLD VNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 61   YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120
            YSNEHYDISEVFL DVRLE+PFV FCLDKS+RENSRYSLRFRLCNVNGSLLDRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 121  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 180
            VLSSND FLE IERD+EEDVKACSVVLSGN DGPDEAISGLVHLANLKLMTLRPVDGV F
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 181  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 240
            SQN GSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTI WLRPEVVLSEVKYGVVKS+
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 241  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 300
            NMDTHLHHE GDD SNSRKH NFDTI FYDAIKPSKDEP LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 301  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 360
            YWM+QREKGFSGNSGLGENVQLISPLCMALKCL+TCLRVFYNPFSGNLTLNAEQ+LPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 361  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 420
            GGILADEMGLGKTIELLACILSHQMSVFEG KGFDVEV+QLVEDQRTEFKRLKRER+ECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 480
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVK VDTEDGNSRREKRNN+NTLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE 540
            VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHT PGSIKLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 540

Query: 541  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
            GVRDTSLLGTLS QINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 541  GVRDTSLLGTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
            ASPFNVH+WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 721  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 780
            LKFSP+EEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCV+SDIPSDLL+THADAGKL
Sbjct: 721  LKFSPVEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVSSDIPSDLLVTHADAGKL 780

Query: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKT+IEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 841  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 900
            AGIAII KKF EAFSLYKEALE AEEN EDFRLDPLLSIHIHHNLAEILPLAVNQSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 901  KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 960
            KDQ CPRTCEVKASRMDDSEKY  HVHIMKKQKVSETLYATCSEDNTGKMIDHPLQL GK
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 961  DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020
            DTNAKKEENYEPHRS+ YFDEI VRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1021 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080
            LKDREN NDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1140
            HVQSGLDLLEASRKVVLDRLLEIDQTM++PKEEDIERVRYCR CQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1200
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEEP 1200

Query: 1201 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1260
            NKRD GEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESIAAANKQLHLLE+MRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLSLRDDDDDSSAFTISEHELPAASVQYSSDKFMSLAMLS 1320
            LAIAQA VLNAHDEIKMATTRL LRD+DDDSSAF+ISEHELPAASVQYSSDKFMSLAMLS
Sbjct: 1261 LAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAMLS 1320

Query: 1321 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQTIPL SSSDLALTQEPAITSTA EQKNENT KADEESCP+CQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQE 1380

Query: 1381 KLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
            KL+NQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 ETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
            ETLDPST LETSR+HEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1501 LHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560
            LHYAF ANNI+FIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI
Sbjct: 1501 LHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIYK 1620
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRF+VKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1620

Query: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLRENDDKGSETLRQLAPSMAAT 1680
            LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPL ENDDKGSETLRQLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAAT 1680

Query: 1681 IAAERRLREHTT 1693
            IAAERRLREHTT
Sbjct: 1681 IAAERRLREHTT 1692

BLAST of CSPI06G02230 vs. ExPASy TrEMBL
Match: A0A1S3C421 (E3 ubiquitin-protein ligase SHPRH isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496631 PE=3 SV=1)

HSP 1 Score: 3125.5 bits (8102), Expect = 0.0e+00
Identity = 1578/1692 (93.26%), Postives = 1607/1692 (94.98%), Query Frame = 0

Query: 1    MGRRKQKQPNRSGGIRFEDRGDPKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 60
            MGRRKQKQP+ SGGIRFEDRGDPKTQLDIN V ETA E+SDIKLD VNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 61   YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120
            YSNEHYDISEVFL DVRLE+PFV FCLDKS+RENSRYSLRFRLCNVNGSLLDRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 121  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 180
            VLSSND FLE IERD+EEDVKACSVVLSGN DGPDEAISGLVHLANLKLMTLRPVDGV F
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 181  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 240
            SQN GSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTI WLRPEVVLSEVKYGVVKS+
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 241  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 300
            NMDTHLHHE GDD SNSRKH NFDTI FYDAIKPSKDEP LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 301  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 360
            YWM+QREKGFSGNSGLGENVQLISPLCMALKCL+TCLRVFYNPFSGNLTLNAEQ+LPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 361  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 420
            GGILADEMGLGKTIELLACILSHQMSVFEG KGFDVEV+QLVEDQRTEFKRLKRER+ECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 480
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVK VDTEDGNSRREKRNN+NTLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE 540
            VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHT PGSIKLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 540

Query: 541  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
            GVRDTSLLGTLS QINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 541  GVRDTSLLGTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
            ASPFNVH+WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 721  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 780
            LKFSP+EEHFYQRQHETC                                         L
Sbjct: 721  LKFSPVEEHFYQRQHETC-----------------------------------------L 780

Query: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKT+IEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 841  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 900
            AGIAII KKF EAFSLYKEALE AEEN EDFRLDPLLSIHIHHNLAEILPLAVNQSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 901  KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 960
            KDQ CPRTCEVKASRMDDSEKY  HVHIMKKQKVSETLYATCSEDNTGKMIDHPLQL GK
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 961  DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020
            DTNAKKEENYEPHRS+ YFDEI VRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1021 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080
            LKDREN NDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1140
            HVQSGLDLLEASRKVVLDRLLEIDQTM++PKEEDIERVRYCR CQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1200
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEEP 1200

Query: 1201 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1260
            NKRD GEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESIAAANKQLHLLE+MRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLSLRDDDDDSSAFTISEHELPAASVQYSSDKFMSLAMLS 1320
            LAIAQA VLNAHDEIKMATTRL LRD+DDDSSAF+ISEHELPAASVQYSSDKFMSLAMLS
Sbjct: 1261 LAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAMLS 1320

Query: 1321 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQTIPL SSSDLALTQEPAITSTA EQKNENT KADEESCP+CQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQE 1380

Query: 1381 KLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
            KL+NQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 ETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
            ETLDPST LETSR+HEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1501 LHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560
            LHYAF ANNI+FIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI
Sbjct: 1501 LHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIYK 1620
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRF+VKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1620

Query: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLRENDDKGSETLRQLAPSMAAT 1680
            LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPL ENDDKGSETLRQLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAAT 1651

Query: 1681 IAAERRLREHTT 1693
            IAAERRLREHTT
Sbjct: 1681 IAAERRLREHTT 1651

BLAST of CSPI06G02230 vs. ExPASy TrEMBL
Match: A0A1S3C4R3 (E3 ubiquitin-protein ligase SHPRH isoform X3 OS=Cucumis melo OX=3656 GN=LOC103496631 PE=3 SV=1)

HSP 1 Score: 3068.1 bits (7953), Expect = 0.0e+00
Identity = 1552/1692 (91.73%), Postives = 1583/1692 (93.56%), Query Frame = 0

Query: 1    MGRRKQKQPNRSGGIRFEDRGDPKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 60
            MGRRKQKQP+ SGGIRFEDRGDPKTQLDIN V ETA E+SDIKLD VNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 61   YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120
            YSNEHYDISEVFL DVRLE+PFV FCLDKS+RENSRYSLRFRLCNVNGSLLDRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 121  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 180
            VLSSND FLE IERD+EEDVKACSVVLSGN DGPDEAISGLVHLANLKLMTLRPVDGV F
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 181  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 240
            SQN GSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTI WLRPEVVLSEVKYGVVKS+
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 241  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 300
            NMDTHLHHE GDD SNSRKH NFDTI FYDAIKPSKDEP LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 301  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 360
            YWM+QREKGFSGNSGLGENVQLISPLCMALKCL+TCLRVFYNPFSGNLTLNAEQ+LPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 361  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 420
            GGILADEMGLGKTIELLACILSHQMSVFEG KGFDVEV+QLVEDQRTEFKRLKRER+ECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 480
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVK VDTEDGNSRREKRNN+NTLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE 540
            VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEIL              
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEIL-------------- 540

Query: 541  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
                                                                 RYPVIPT
Sbjct: 541  -----------------------------------------------------RYPVIPT 600

Query: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
            ASPFNVH+WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 721  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 780
            LKFSP+EEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCV+SDIPSDLL+THADAGKL
Sbjct: 721  LKFSPVEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVSSDIPSDLLVTHADAGKL 780

Query: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKT+IEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 841  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 900
            AGIAII KKF EAFSLYKEALE AEEN EDFRLDPLLSIHIHHNLAEILPLAVNQSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 901  KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 960
            KDQ CPRTCEVKASRMDDSEKY  HVHIMKKQKVSETLYATCSEDNTGKMIDHPLQL GK
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 961  DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020
            DTNAKKEENYEPHRS+ YFDEI VRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1021 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080
            LKDREN NDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1140
            HVQSGLDLLEASRKVVLDRLLEIDQTM++PKEEDIERVRYCR CQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1200
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEEP 1200

Query: 1201 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1260
            NKRD GEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESIAAANKQLHLLE+MRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLSLRDDDDDSSAFTISEHELPAASVQYSSDKFMSLAMLS 1320
            LAIAQA VLNAHDEIKMATTRL LRD+DDDSSAF+ISEHELPAASVQYSSDKFMSLAMLS
Sbjct: 1261 LAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAMLS 1320

Query: 1321 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQTIPL SSSDLALTQEPAITSTA EQKNENT KADEESCP+CQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQE 1380

Query: 1381 KLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
            KL+NQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 ETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
            ETLDPST LETSR+HEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1501 LHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560
            LHYAF ANNI+FIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI
Sbjct: 1501 LHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIYK 1620
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRF+VKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1620

Query: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLRENDDKGSETLRQLAPSMAAT 1680
            LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPL ENDDKGSETLRQLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAAT 1625

Query: 1681 IAAERRLREHTT 1693
            IAAERRLREHTT
Sbjct: 1681 IAAERRLREHTT 1625

BLAST of CSPI06G02230 vs. ExPASy TrEMBL
Match: A0A6J1FE16 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444885 PE=3 SV=1)

HSP 1 Score: 2892.4 bits (7497), Expect = 0.0e+00
Identity = 1455/1692 (85.99%), Postives = 1557/1692 (92.02%), Query Frame = 0

Query: 1    MGRRKQKQPNRSGGIRFEDRGDPKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 60
            MGRRKQKQP+RSGGIRFED   PKTQ+DIN +VET VESSDIKLD VNEP+FVEVDR+GW
Sbjct: 1    MGRRKQKQPHRSGGIRFEDPVSPKTQVDINGIVET-VESSDIKLDEVNEPLFVEVDRTGW 60

Query: 61   YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120
            +SNEH+DISEV L D+RLEHPFVG   ++   E+SRYSLRFRLCN NG LLDRIKFGHWP
Sbjct: 61   HSNEHHDISEVVLMDIRLEHPFVGIQSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWP 120

Query: 121  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 180
            VLSSND  LE IER +EED+  CSVVLSG+ DGPDEAISGLVHLA+LK +TLRPV GV F
Sbjct: 121  VLSSNDISLELIERRVEEDMNGCSVVLSGSFDGPDEAISGLVHLAHLKFVTLRPVMGVSF 180

Query: 181  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 240
            SQNMGSLRLRVEILSSAFDAC SI D+GRQLWKKSMMNTI WLRPEVV SEVKYGVV S+
Sbjct: 181  SQNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVGST 240

Query: 241  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 300
            NMD HLH E GDD S+SRKH NFD++ FYDAIKPSKD+PMLDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAA 300

Query: 301  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 360
            YWM+QREKGFS N G+GENVQLISPLCM L+CLDT  R+FYNPFSG+LT NAEQ+LPHVY
Sbjct: 301  YWMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQILPHVY 360

Query: 361  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 420
            GGILADEMGLGKTIELLACILSH+MSVFE  KG D E+Q LV DQRT+FKRLKRER+EC+
Sbjct: 361  GGILADEMGLGKTIELLACILSHRMSVFESGKGLDDELQPLVGDQRTKFKRLKRERVECV 420

Query: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 480
            CGAVSEN+RY+GLWVQCDICDAWQHADCVGYSPKGRI++ VDT+DGN R++KRN RNT+N
Sbjct: 421  CGAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKRNKRNTVN 480

Query: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE 540
            V VR EEHVC PCLELMQATDSPM TGATLIVCPAPILFQW AEILRHTHPGSIKLLVYE
Sbjct: 481  VTVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYE 540

Query: 541  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
            GVRDTSL  T   QIND+INSDIVLTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PT
Sbjct: 541  GVRDTSLSNTSPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPT 600

Query: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660

Query: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
            ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLK+HVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTW 720

Query: 721  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 780
            L FSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKVP  V+SD+PSD+LITHADAGKL
Sbjct: 721  LTFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKL 780

Query: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
            L+TLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR+VVALNAL
Sbjct: 781  LNTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNAL 840

Query: 841  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 900
            AGIAIIEKKFSEA SLYKEALE AEENNEDFR+DPLLSIHIHHNLAEILPLA++Q QSPL
Sbjct: 841  AGIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPL 900

Query: 901  KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 960
            KDQ  PRTCEVKAS+MDDSEK D+  HI+KKQKV+ TLYA CS+  T K+ID PL+L G 
Sbjct: 901  KDQFYPRTCEVKASKMDDSEKSDN--HIIKKQKVNGTLYAPCSD--TEKIIDSPLELTGL 960

Query: 961  DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020
            D NAKKEENYEPHRSS YF++  +RK CE MRQKYLAVFSSKL IAQQEFTKSY+QV +E
Sbjct: 961  DENAKKEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVCNE 1020

Query: 1021 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080
            LK REN N+VWWL+AVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSI ALKY
Sbjct: 1021 LKSRENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSIDALKY 1080

Query: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1140
            HVQ+GLDLLEASRK+V+ RLLEIDQTM+SPK+EDIERVRYC+ CQADSNGPPCVLCELDE
Sbjct: 1081 HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1200
            LFQEYEARLFRLN VQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKN SSS+ G+EE 
Sbjct: 1141 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1200

Query: 1201 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1260
            NKRDAGE+V+VSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLE+MRKEYGHARS
Sbjct: 1201 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLSLRDDDDDSSAFTISEHELPAASVQYSSDKFMSLAMLS 1320
            LAIAQAQVLNAHDEIKMATTRL LR +DDD SA+ ISEHELPAASVQYSS+KF+SLA LS
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLS 1320

Query: 1321 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQ IPL SSS+ A TQ P ITST+ EQK+ENT KADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQE 1380

Query: 1381 KLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
             L+NQKMVFQCGH+TCCKCLFAMTEK L  S IQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 TLSNQKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 ETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
            ETLDPST  +TSRE E SI VQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1501 LHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560
            L YAF ANNITF+RMKGGRKS TAISEFRGQKINAKEN KK+   ++PE+RSAQVLLLLI
Sbjct: 1501 LQYAFAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIYK 1620
            QHGANGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRF+VKDTVEESI++
Sbjct: 1561 QHGANGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHR 1620

Query: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLRENDDKGSETLRQLAPSMAAT 1680
            LNRSRESSSFITGN KNQDQP  TLKEVESLFAS+ SPLRE+D+K SETLR LAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAAT 1680

Query: 1681 IAAERRLREHTT 1693
            IAAERRL+EHTT
Sbjct: 1681 IAAERRLKEHTT 1687

BLAST of CSPI06G02230 vs. NCBI nr
Match: XP_011656441.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucumis sativus] >KGN45843.2 hypothetical protein Csa_005571 [Cucumis sativus])

HSP 1 Score: 3355.8 bits (8700), Expect = 0.0e+00
Identity = 1688/1693 (99.70%), Postives = 1690/1693 (99.82%), Query Frame = 0

Query: 1    MGRRKQKQPNRSGGIRFEDRGDPKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 60
            MGRRKQKQPNRSGGIRFEDRGD KTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPNRSGGIRFEDRGDHKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 60

Query: 61   YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120
            YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 121  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 180
            VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF
Sbjct: 121  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 180

Query: 181  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 240
            SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS
Sbjct: 181  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 240

Query: 241  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 300
            NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 300

Query: 301  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 360
            YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY
Sbjct: 301  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 360

Query: 361  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 420
            GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 420

Query: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 480
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 480

Query: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE 540
            VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGS+KLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSMKLLVYE 540

Query: 541  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
            GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 541  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660

Query: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
            ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 721  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 780
            LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL
Sbjct: 721  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 780

Query: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840

Query: 841  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 900
            AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL
Sbjct: 841  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 900

Query: 901  KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 960
            KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK
Sbjct: 901  KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 960

Query: 961  DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020
            DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE
Sbjct: 961  DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1021 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080
            LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1140
            HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1200
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1200

Query: 1201 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1260
            NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS
Sbjct: 1201 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLSLR-DDDDDSSAFTISEHELPAASVQYSSDKFMSLAML 1320
            LAIAQAQVLNAHDEIKMATTRLSLR DDDDDSSAFTISEHELPAASVQYSSDKFMSLAML
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLSLRGDDDDDSSAFTISEHELPAASVQYSSDKFMSLAML 1320

Query: 1321 SRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQ 1380
            SRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQ
Sbjct: 1321 SRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQ 1380

Query: 1381 EKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSK 1440
            EKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSK
Sbjct: 1381 EKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSK 1440

Query: 1441 NETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLD 1500
            NETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLD
Sbjct: 1441 NETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLD 1500

Query: 1501 VLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLL 1560
            VLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLL
Sbjct: 1501 VLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLL 1560

Query: 1561 IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIY 1620
            IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRF+VKDTVEESIY
Sbjct: 1561 IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIY 1620

Query: 1621 KLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLRENDDKGSETLRQLAPSMAA 1680
            KLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPL ENDDKGSETLRQLAPSMAA
Sbjct: 1621 KLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAA 1680

Query: 1681 TIAAERRLREHTT 1693
            TIAAERRLREHTT
Sbjct: 1681 TIAAERRLREHTT 1693

BLAST of CSPI06G02230 vs. NCBI nr
Match: XP_008456785.1 (PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucumis melo] >XP_008456786.1 PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucumis melo] >XP_008456787.1 PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucumis melo] >XP_016901994.1 PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucumis melo])

HSP 1 Score: 3226.0 bits (8363), Expect = 0.0e+00
Identity = 1617/1692 (95.57%), Postives = 1648/1692 (97.40%), Query Frame = 0

Query: 1    MGRRKQKQPNRSGGIRFEDRGDPKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 60
            MGRRKQKQP+ SGGIRFEDRGDPKTQLDIN V ETA E+SDIKLD VNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 61   YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120
            YSNEHYDISEVFL DVRLE+PFV FCLDKS+RENSRYSLRFRLCNVNGSLLDRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 121  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 180
            VLSSND FLE IERD+EEDVKACSVVLSGN DGPDEAISGLVHLANLKLMTLRPVDGV F
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 181  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 240
            SQN GSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTI WLRPEVVLSEVKYGVVKS+
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 241  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 300
            NMDTHLHHE GDD SNSRKH NFDTI FYDAIKPSKDEP LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 301  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 360
            YWM+QREKGFSGNSGLGENVQLISPLCMALKCL+TCLRVFYNPFSGNLTLNAEQ+LPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 361  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 420
            GGILADEMGLGKTIELLACILSHQMSVFEG KGFDVEV+QLVEDQRTEFKRLKRER+ECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 480
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVK VDTEDGNSRREKRNN+NTLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE 540
            VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHT PGSIKLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 540

Query: 541  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
            GVRDTSLLGTLS QINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 541  GVRDTSLLGTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
            ASPFNVH+WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 721  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 780
            LKFSP+EEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCV+SDIPSDLL+THADAGKL
Sbjct: 721  LKFSPVEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVSSDIPSDLLVTHADAGKL 780

Query: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKT+IEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 841  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 900
            AGIAII KKF EAFSLYKEALE AEEN EDFRLDPLLSIHIHHNLAEILPLAVNQSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 901  KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 960
            KDQ CPRTCEVKASRMDDSEKY  HVHIMKKQKVSETLYATCSEDNTGKMIDHPLQL GK
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 961  DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020
            DTNAKKEENYEPHRS+ YFDEI VRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1021 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080
            LKDREN NDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1140
            HVQSGLDLLEASRKVVLDRLLEIDQTM++PKEEDIERVRYCR CQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1200
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEEP 1200

Query: 1201 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1260
            NKRD GEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESIAAANKQLHLLE+MRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLSLRDDDDDSSAFTISEHELPAASVQYSSDKFMSLAMLS 1320
            LAIAQA VLNAHDEIKMATTRL LRD+DDDSSAF+ISEHELPAASVQYSSDKFMSLAMLS
Sbjct: 1261 LAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAMLS 1320

Query: 1321 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQTIPL SSSDLALTQEPAITSTA EQKNENT KADEESCP+CQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQE 1380

Query: 1381 KLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
            KL+NQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 ETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
            ETLDPST LETSR+HEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1501 LHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560
            LHYAF ANNI+FIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI
Sbjct: 1501 LHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIYK 1620
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRF+VKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1620

Query: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLRENDDKGSETLRQLAPSMAAT 1680
            LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPL ENDDKGSETLRQLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAAT 1680

Query: 1681 IAAERRLREHTT 1693
            IAAERRLREHTT
Sbjct: 1681 IAAERRLREHTT 1692

BLAST of CSPI06G02230 vs. NCBI nr
Match: XP_008456789.1 (PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X2 [Cucumis melo])

HSP 1 Score: 3125.5 bits (8102), Expect = 0.0e+00
Identity = 1578/1692 (93.26%), Postives = 1607/1692 (94.98%), Query Frame = 0

Query: 1    MGRRKQKQPNRSGGIRFEDRGDPKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 60
            MGRRKQKQP+ SGGIRFEDRGDPKTQLDIN V ETA E+SDIKLD VNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 61   YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120
            YSNEHYDISEVFL DVRLE+PFV FCLDKS+RENSRYSLRFRLCNVNGSLLDRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 121  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 180
            VLSSND FLE IERD+EEDVKACSVVLSGN DGPDEAISGLVHLANLKLMTLRPVDGV F
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 181  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 240
            SQN GSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTI WLRPEVVLSEVKYGVVKS+
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 241  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 300
            NMDTHLHHE GDD SNSRKH NFDTI FYDAIKPSKDEP LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 301  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 360
            YWM+QREKGFSGNSGLGENVQLISPLCMALKCL+TCLRVFYNPFSGNLTLNAEQ+LPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 361  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 420
            GGILADEMGLGKTIELLACILSHQMSVFEG KGFDVEV+QLVEDQRTEFKRLKRER+ECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 480
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVK VDTEDGNSRREKRNN+NTLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE 540
            VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHT PGSIKLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 540

Query: 541  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
            GVRDTSLLGTLS QINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 541  GVRDTSLLGTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
            ASPFNVH+WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 721  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 780
            LKFSP+EEHFYQRQHETC                                         L
Sbjct: 721  LKFSPVEEHFYQRQHETC-----------------------------------------L 780

Query: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKT+IEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 841  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 900
            AGIAII KKF EAFSLYKEALE AEEN EDFRLDPLLSIHIHHNLAEILPLAVNQSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 901  KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 960
            KDQ CPRTCEVKASRMDDSEKY  HVHIMKKQKVSETLYATCSEDNTGKMIDHPLQL GK
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 961  DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020
            DTNAKKEENYEPHRS+ YFDEI VRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1021 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080
            LKDREN NDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1140
            HVQSGLDLLEASRKVVLDRLLEIDQTM++PKEEDIERVRYCR CQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1200
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEEP 1200

Query: 1201 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1260
            NKRD GEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESIAAANKQLHLLE+MRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLSLRDDDDDSSAFTISEHELPAASVQYSSDKFMSLAMLS 1320
            LAIAQA VLNAHDEIKMATTRL LRD+DDDSSAF+ISEHELPAASVQYSSDKFMSLAMLS
Sbjct: 1261 LAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAMLS 1320

Query: 1321 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQTIPL SSSDLALTQEPAITSTA EQKNENT KADEESCP+CQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQE 1380

Query: 1381 KLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
            KL+NQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 ETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
            ETLDPST LETSR+HEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1501 LHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560
            LHYAF ANNI+FIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI
Sbjct: 1501 LHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIYK 1620
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRF+VKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1620

Query: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLRENDDKGSETLRQLAPSMAAT 1680
            LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPL ENDDKGSETLRQLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAAT 1651

Query: 1681 IAAERRLREHTT 1693
            IAAERRLREHTT
Sbjct: 1681 IAAERRLREHTT 1651

BLAST of CSPI06G02230 vs. NCBI nr
Match: XP_038884903.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida] >XP_038884904.1 E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida])

HSP 1 Score: 3095.1 bits (8023), Expect = 0.0e+00
Identity = 1554/1692 (91.84%), Postives = 1610/1692 (95.15%), Query Frame = 0

Query: 1    MGRRKQKQPNRSGGIRFEDRGDPKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 60
            MGRRKQ+QP+RSGGIRFEDRGDPKTQ+DIN     AVESSDIK+  VNEPIFVEVDR+GW
Sbjct: 1    MGRRKQRQPHRSGGIRFEDRGDPKTQIDIN----GAVESSDIKVAEVNEPIFVEVDRTGW 60

Query: 61   YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120
             SNEH+DISEVFL DVRLEHPFV F LDKS  ENSRYSLRFRLCNVNGSLLDRIKFGHWP
Sbjct: 61   NSNEHHDISEVFLMDVRLEHPFVDFRLDKSFCENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 121  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 180
            VLSSND  LE IERDMEED+KACSVVLSGN DGPDEAISGLVHLANLK  TLRPV+GV  
Sbjct: 121  VLSSNDILLELIERDMEEDMKACSVVLSGNFDGPDEAISGLVHLANLKFATLRPVEGVSV 180

Query: 181  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 240
            SQN GSLRLRVEILSSAF ACESIFDNGRQLWKKSMMNTI WLRPEVVLSEVKYGVVKS+
Sbjct: 181  SQNTGSLRLRVEILSSAFAACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 241  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 300
            NMDT LHH  GDDTSNSRKH NFDT+ FYDAIKPSKDEPMLDEDIPNLLPKLR YQRRAA
Sbjct: 241  NMDTSLHHGTGDDTSNSRKHVNFDTVAFYDAIKPSKDEPMLDEDIPNLLPKLRQYQRRAA 300

Query: 301  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 360
            YWM+QREKGFSGN G+GENVQLISPLCMALKC+DTC RVFYNPFSGNLTLNAEQ LPHVY
Sbjct: 301  YWMVQREKGFSGNLGVGENVQLISPLCMALKCVDTCSRVFYNPFSGNLTLNAEQTLPHVY 360

Query: 361  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 420
            GGILADEMGLGKTIELLACILSHQMSVFEG KGF  E+QQ VEDQRTEFKRLKRER+ECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFHDELQQPVEDQRTEFKRLKRERVECL 420

Query: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 480
            CGAVSEN+RYKGLWVQCDICDAWQHADCVGYSPKGRI+K +DTE GN R+EKRNNRNTLN
Sbjct: 421  CGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRILKPIDTEGGNCRKEKRNNRNTLN 480

Query: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE 540
            VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE 540

Query: 541  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
            GVRDTSLL TLS QINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 541  GVRDTSLLSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660

Query: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
            ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 721  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 780
            L FSPIEEHFYQRQHETCVSYAREVIQGLKDDFVK+KVPDC++SDIPSDLLI HADAGKL
Sbjct: 721  LTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKKKVPDCISSDIPSDLLINHADAGKL 780

Query: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840

Query: 841  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 900
            AGIAIIEKKFSEAFSLYKEALE AEENNEDFRLDPLLSIHIHHNLAEILPLAV+QSQS L
Sbjct: 841  AGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVDQSQSRL 900

Query: 901  KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 960
            KDQ CPRT EVKA+RMDDSEKYD+HVH+MKKQKVSETLYA CSEDNTGKMID PL+L  K
Sbjct: 901  KDQFCPRTSEVKAARMDDSEKYDNHVHVMKKQKVSETLYAPCSEDNTGKMIDRPLELTRK 960

Query: 961  DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020
            DTN KKEEN EPH SS YFD+IS+RK CE MRQKYLAVFSSKLS+AQQEFTKSYMQVGSE
Sbjct: 961  DTNTKKEENCEPHLSSSYFDDISIRKACEAMRQKYLAVFSSKLSVAQQEFTKSYMQVGSE 1020

Query: 1021 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080
            LKDR N N VWWLEAVHHAEQNKDFSHELIRK+EEAVSGNLNNSKSRVGSRFRSI+ALKY
Sbjct: 1021 LKDRGNLNHVWWLEAVHHAEQNKDFSHELIRKVEEAVSGNLNNSKSRVGSRFRSINALKY 1080

Query: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1140
            HVQSGLDLLEASRKVVLDRLLEIDQTM++PKEEDIERVRYCR CQADSNGPPCVLCELD+
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDD 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1200
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSS+VG+EEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSRVGYEEP 1200

Query: 1201 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1260
            NKRD GEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLE+MRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLSLRDDDDDSSAFTISEHELPAASVQYSSDKFMSLAMLS 1320
            LAIAQAQVLNAHDEIKMATTRL LR DDDDSSA+ ISEHELPAASVQYSSDKFMSLAML+
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLR-DDDDSSAYAISEHELPAASVQYSSDKFMSLAMLA 1320

Query: 1321 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQ IPLDSSS+LALTQ+PA  ST+ EQK+ENTSKADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLDSSSNLALTQQPATMSTSMEQKSENTSKADEESCPVCQE 1380

Query: 1381 KLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
            KL+NQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 ETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
            ETLDPST  ETSREHE+SITVQGSYGTK+EAVVRRILWIKYTDS+AKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHETSREHELSITVQGSYGTKVEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500

Query: 1501 LHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560
            L YAF AN+ITFIRMKGGR+S TAISEFRGQKINAKEN+KKR S  +PELRSAQVLLLLI
Sbjct: 1501 LQYAFAANSITFIRMKGGRQSHTAISEFRGQKINAKENQKKRPSGKVPELRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIYK 1620
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRF+VKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQKNKTFVHRFIVKDTVEESIYK 1620

Query: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLRENDDKGSETLRQLAPSMAAT 1680
            LNRSRESSSFITGN KNQDQP LTLKEVESLFASKASPLREND+KGSETLR LAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPLLTLKEVESLFASKASPLRENDEKGSETLRHLAPSMAAT 1680

Query: 1681 IAAERRLREHTT 1693
            IAAERRL+ HTT
Sbjct: 1681 IAAERRLKVHTT 1687

BLAST of CSPI06G02230 vs. NCBI nr
Match: XP_008456790.1 (PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X3 [Cucumis melo])

HSP 1 Score: 3068.1 bits (7953), Expect = 0.0e+00
Identity = 1552/1692 (91.73%), Postives = 1583/1692 (93.56%), Query Frame = 0

Query: 1    MGRRKQKQPNRSGGIRFEDRGDPKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 60
            MGRRKQKQP+ SGGIRFEDRGDPKTQLDIN V ETA E+SDIKLD VNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 61   YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120
            YSNEHYDISEVFL DVRLE+PFV FCLDKS+RENSRYSLRFRLCNVNGSLLDRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 121  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 180
            VLSSND FLE IERD+EEDVKACSVVLSGN DGPDEAISGLVHLANLKLMTLRPVDGV F
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 181  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 240
            SQN GSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTI WLRPEVVLSEVKYGVVKS+
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 241  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 300
            NMDTHLHHE GDD SNSRKH NFDTI FYDAIKPSKDEP LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 301  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 360
            YWM+QREKGFSGNSGLGENVQLISPLCMALKCL+TCLRVFYNPFSGNLTLNAEQ+LPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 361  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 420
            GGILADEMGLGKTIELLACILSHQMSVFEG KGFDVEV+QLVEDQRTEFKRLKRER+ECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 480
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVK VDTEDGNSRREKRNN+NTLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE 540
            VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEIL              
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEIL-------------- 540

Query: 541  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
                                                                 RYPVIPT
Sbjct: 541  -----------------------------------------------------RYPVIPT 600

Query: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
            ASPFNVH+WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 721  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 780
            LKFSP+EEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCV+SDIPSDLL+THADAGKL
Sbjct: 721  LKFSPVEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVSSDIPSDLLVTHADAGKL 780

Query: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKT+IEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 841  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 900
            AGIAII KKF EAFSLYKEALE AEEN EDFRLDPLLSIHIHHNLAEILPLAVNQSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 901  KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 960
            KDQ CPRTCEVKASRMDDSEKY  HVHIMKKQKVSETLYATCSEDNTGKMIDHPLQL GK
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 961  DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020
            DTNAKKEENYEPHRS+ YFDEI VRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1021 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080
            LKDREN NDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1140
            HVQSGLDLLEASRKVVLDRLLEIDQTM++PKEEDIERVRYCR CQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1200
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEEP 1200

Query: 1201 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1260
            NKRD GEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESIAAANKQLHLLE+MRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLSLRDDDDDSSAFTISEHELPAASVQYSSDKFMSLAMLS 1320
            LAIAQA VLNAHDEIKMATTRL LRD+DDDSSAF+ISEHELPAASVQYSSDKFMSLAMLS
Sbjct: 1261 LAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAMLS 1320

Query: 1321 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQTIPL SSSDLALTQEPAITSTA EQKNENT KADEESCP+CQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQE 1380

Query: 1381 KLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
            KL+NQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 ETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
            ETLDPST LETSR+HEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1501 LHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560
            LHYAF ANNI+FIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI
Sbjct: 1501 LHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIYK 1620
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRF+VKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1620

Query: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLRENDDKGSETLRQLAPSMAAT 1680
            LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPL ENDDKGSETLRQLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAAT 1625

Query: 1681 IAAERRLREHTT 1693
            IAAERRLREHTT
Sbjct: 1681 IAAERRLREHTT 1625

BLAST of CSPI06G02230 vs. TAIR 10
Match: AT2G40770.1 (zinc ion binding;DNA binding;helicases;ATP binding;nucleic acid binding )

HSP 1 Score: 1758.0 bits (4552), Expect = 0.0e+00
Identity = 959/1710 (56.08%), Postives = 1209/1710 (70.70%), Query Frame = 0

Query: 1    MGRRKQKQPNRSGGIRFEDRGDPKTQLDINEVV-ETAVESSDIKLDGVNEPIFVEVDRSG 60
            MGRRKQ +P R+ G+     G   ++ D N++  + A  S D  ++ +++P +V +  S 
Sbjct: 1    MGRRKQSKPQRAVGL-ITPTG---SESDRNQLPGDEAEGSGDKNVEDIDKPYYVNICLSS 60

Query: 61   WYS--NEHYDISEVFLADVRLEHPFVG----FCLDKSVRENSRYSLRFRLCNVNGSLLDR 120
              S   +++D++EV L +  +    VG    F     V  +   SLRFRLCNV  + +DR
Sbjct: 61   RVSEQQQNFDLAEVVLTNFSVRER-VGSSSTFTKPIEVDHDLDCSLRFRLCNVT-NFVDR 120

Query: 121  IKFGHWPVLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLR 180
            IK GHWPVLSS+D  LE ++  + +D +  SV+ S + DGP E +SGL HLA++K +TLR
Sbjct: 121  IKLGHWPVLSSSDITLELVDNKVSDD-EVGSVIWSASFDGPGEGVSGLAHLASIKFLTLR 180

Query: 181  PVDGVPFSQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVK 240
             + G   ++ + S R+RVE+L  AFDAC+S+ +N RQ+WKKSM++ ++WLRPEV+ SE +
Sbjct: 181  LMPG---NEGLLSPRVRVEMLQQAFDACDSLLENTRQIWKKSMIHVMSWLRPEVMTSEAR 240

Query: 241  YGVVKSSNMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLR 300
            YG  + +  D        D+T +S K ++FD   FY+AIKPSK + ML +DI +LLP+LR
Sbjct: 241  YG-TRFNVKDIESSVVTEDETLDSSKQSSFDAAAFYEAIKPSKTDAMLGDDIADLLPELR 300

Query: 301  PYQRRAAYWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAE 360
            PYQRRAAYWM+QRE+G     G  E+ Q ISPL +++  LD+  ++F NPFSGN++L  E
Sbjct: 301  PYQRRAAYWMVQRERGDPITLGDKEDNQFISPLSISVGFLDSATKMFLNPFSGNISLTPE 360

Query: 361  QVLPHVYGGILADEMGLGKTIELLACILSH------QMSVFEGRKGFDVEVQQLVEDQRT 420
               P + GGILADEMGLGKT+ELLACI SH      ++SV  G    DV           
Sbjct: 361  YFSPRIQGGILADEMGLGKTVELLACIFSHRKPAEDEISVSNGSSVTDV--------LNA 420

Query: 421  EFKRLKRERIECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGN 480
              +RLKRER+EC+CGAVSE+++YKG+WVQCD+CDAWQHADCVGYSPKG+  K     D  
Sbjct: 421  GLRRLKRERVECICGAVSESHKYKGVWVQCDLCDAWQHADCVGYSPKGKGKKDSQHID-E 480

Query: 481  SRREKRNNRNTLNVIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILR 540
               +K++ ++   +IVR  E++C  C EL+Q T SP++TGATLIVCPAPIL QW +EI R
Sbjct: 481  KASQKKSKKDATEIIVREGEYICQMCSELLQVTASPISTGATLIVCPAPILPQWHSEITR 540

Query: 541  HTHPGSIKLLVYEGVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRR 600
            HT  GS+   +YEGVR+ SL       I +L+N+DIVLT+YDVLKEDL+HD DRH+GDR 
Sbjct: 541  HTRLGSLITCIYEGVRNASLSEEPMIDITELLNADIVLTTYDVLKEDLTHDFDRHDGDRH 600

Query: 601  FMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQR 660
             +RFQKRYPVIPTPLTRIFWWRICLDE QMVESNA AATEMA RLY  HRWCITGTPIQR
Sbjct: 601  CLRFQKRYPVIPTPLTRIFWWRICLDEAQMVESNAAAATEMALRLYTKHRWCITGTPIQR 660

Query: 661  KLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTD 720
            KL+DL+GLL+F+KA+PF+V RWW+EVIRDPYERRD  AMEFTHKFFKQ+MWRS K+HV D
Sbjct: 661  KLDDLFGLLKFLKANPFDVSRWWIEVIRDPYERRDTKAMEFTHKFFKQVMWRSSKVHVAD 720

Query: 721  ELQLPPQEEQVTWLKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIP 780
            ELQLPPQEE V+WLKFS IEEHFY RQH+TCVSYAREVI+ LK D +KR      +SD P
Sbjct: 721  ELQLPPQEECVSWLKFSAIEEHFYSRQHDTCVSYAREVIETLKRDILKR---GHTSSDNP 780

Query: 781  SDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGE 840
               L+THA+A KLL++LLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLV KT+ EGE
Sbjct: 781  ---LVTHAEAAKLLNSLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVKKTQSEGE 840

Query: 841  EALRRSVVALNALAGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAE 900
            EALR  +VALN +A IA+++++FSEA SLYKEAL   EE+ EDFRLDPLL+IHI HNLAE
Sbjct: 841  EALRVLIVALNGIAAIAMLKQEFSEAVSLYKEALSITEEHAEDFRLDPLLNIHILHNLAE 900

Query: 901  ILPLAVNQSQSPLKDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNT 960
            ILP+A +     L     P T      ++D   K DDH    K+Q+++E    T     T
Sbjct: 901  ILPMAKSYG-GKLSASGRPET------KID--VKDDDHHRASKRQRINELESLTHDSSET 960

Query: 961  GKMIDHPLQLKGKDTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQ 1020
                 H  +    D   KK+   E H      D      VC+ ++ KYL+ F+SKLS AQ
Sbjct: 961  ----VHQREAIAPDNGLKKDG--ECHEECKTLD-----IVCDTLKVKYLSAFNSKLSAAQ 1020

Query: 1021 QEFTKSYMQVGSELKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNN-SKS 1080
             EF KSY QV   L +      VWWL+A+   EQNKDFS EL RKIEEA+ GNLNN S S
Sbjct: 1021 HEFKKSYNQVSESLSNMGKQRSVWWLDALQLTEQNKDFSSELTRKIEEALHGNLNNSSSS 1080

Query: 1081 RVGSRFRSISALKYHVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQA 1140
            R  SRFR+I  +K H+Q+ +D+LE SRK V+DR+LEIDQTM+ PK EDIER+  C+ C  
Sbjct: 1081 RESSRFRTIHGMKLHLQTCMDMLERSRKKVIDRILEIDQTMEKPKLEDIERISNCKYCNK 1140

Query: 1141 DSNGPPCVLCELDELFQEYEARLFRLNKVQGGM--VTSVEEAVEAQKKKSALNRFYWSLL 1200
            +S+GPPC+ CELDELFQEYEARLFRLNK + G+  + + EE V  QKK+ A N F + L 
Sbjct: 1141 NSDGPPCIHCELDELFQEYEARLFRLNKSRRGVMEIAAAEETVHLQKKRDARNLFLFGLS 1200

Query: 1201 QQNKNSSSSKVGHEEPNKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQ 1260
             ++K+ ++S+   EEP KR+AG+ V++SK PSE E+VLGVI+N CKT L +ES  AA K 
Sbjct: 1201 SRSKDLNASRGDDEEPTKRNAGDIVVLSKSPSETEIVLGVIRNHCKTHLDRESKLAATKH 1260

Query: 1261 LHLLEHMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLSLRDDDDDSSAFTISEHELPAA 1320
            LH LE MRKEY HAR LA  QAQ+L A+DEI M+T RL LR+ +DD+S + +   EL  A
Sbjct: 1261 LHTLEVMRKEYVHARVLARDQAQLLRAYDEINMSTMRLQLRESEDDTSIYALGRDELDVA 1320

Query: 1321 SVQYSSDKFMSLAMLSRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKN 1380
            SV  ++DKFM+ + L  +KGKLRYLKGL++SKQ    +S    +   E    S   EQ++
Sbjct: 1321 SVLNTNDKFMAQSSLLSIKGKLRYLKGLMKSKQKQESESPDLSSPIHETVDASDPAEQES 1380

Query: 1381 ENTSKADEESCPVCQEKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCR 1440
            EN  K D E+CP+C E L NQKMVFQCGH TCC C FAMTE+      +Q KWVMCP CR
Sbjct: 1381 ENLLKRD-EACPICHEILRNQKMVFQCGHSTCCNCFFAMTERKSVQETLQ-KWVMCPICR 1440

Query: 1441 QHTDFGNIAYADDSKNETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDS 1500
            QHTD  NIAYADD +N     S+S +  ++ E S+ VQGSYGTKIEAV RRILWIK +D 
Sbjct: 1441 QHTDVRNIAYADDRRNS----SSSDQDHKDSEASLVVQGSYGTKIEAVTRRILWIKSSDP 1500

Query: 1501 EAKVLVFSSWNDVLDVLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLS 1560
            + KVLVFSSWNDVLDVL +AF AN+IT IRMKGGRKSQTAIS+F+G      E + ++ +
Sbjct: 1501 QTKVLVFSSWNDVLDVLEHAFAANSITCIRMKGGRKSQTAISKFKG-----SEKETQKTN 1560

Query: 1561 TDLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTF 1620
            +   E +S QVLLLL+QHGANGLNLLEAQHV+LVEPLLNPAAEAQA+ RVHRIGQE  T 
Sbjct: 1561 SHQKEEKSIQVLLLLVQHGANGLNLLEAQHVILVEPLLNPAAEAQAVGRVHRIGQEKPTL 1620

Query: 1621 VHRFVVKDTVEESIYKLNRSRES--SSFITGNAKNQDQPFLTLKEVESLFAS---KASPL 1680
            VHRF+V  TVEESIYKLNR++ +  SSF + N KNQDQ FLTLK++ESLFAS   + + +
Sbjct: 1621 VHRFLVSGTVEESIYKLNRNKNTNLSSFSSRNTKNQDQQFLTLKDLESLFASPTAETAEM 1653

Query: 1681 RENDDKGSETLRQLAPSMAATIAAERRLRE 1690
             +N  +  E LR L PS+AA +AAERR++E
Sbjct: 1681 EQNPGERQENLRDLPPSVAAALAAERRMKE 1653

BLAST of CSPI06G02230 vs. TAIR 10
Match: AT5G22750.1 (DNA/RNA helicase protein )

HSP 1 Score: 178.3 bits (451), Expect = 5.1e-44
Identity = 157/573 (27.40%), Postives = 236/573 (41.19%), Query Frame = 0

Query: 237 VKSSNMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPN-LLPKLRPY 296
           VK+ N D +     GD+        + D I     +       + + + P+ LL +LRPY
Sbjct: 302 VKNMNQDAN-----GDENEQCISDGDLDNI-----VGVGDSSGLKEMETPHTLLCELRPY 361

Query: 297 QRRAAYWMIQREKGFSGNSGLGENVQLISPLCMALKCL--DTCLRVFYNPFSGNLTLNAE 356
           Q++A +WM Q EKG    +   E   ++ P C    CL     L V+ N F+G+ T++  
Sbjct: 362 QKQALHWMTQLEKG----NCTDEAATMLHP-CWEAYCLADKRELVVYLNSFTGDATIHFP 421

Query: 357 QVLPHVYGGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLK 416
             L    GGILAD MGLGKT+  ++ +L+H                              
Sbjct: 422 STLQMARGGILADAMGLGKTVMTISLLLAH------------------------------ 481

Query: 417 RERIECLCGAVSENYRYKGLWVQCDICDAWQHAD----CVGYSPKGRIVKSVDTEDGNSR 476
                                       +W+ A     C  Y     I  SVD       
Sbjct: 482 ----------------------------SWKAASTGFLCPNYEGDKVISSSVD------- 541

Query: 477 REKRNNRNTLNVIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHT 536
                  +  +  V+A + +      L Q   S +  G  LIVCP  +L QW+ EI  H 
Sbjct: 542 -------DLTSPPVKATKFLGFDKRLLEQ--KSVLQNGGNLIVCPMTLLGQWKTEIEMHA 601

Query: 537 HPGSIKLLVYEG---VRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHD-SDRHEGD 596
            PGS+ + V+ G    +D  LL            SD+V+T+Y VL  + S + S  HEG 
Sbjct: 602 KPGSLSVYVHYGQSRPKDAKLLS----------QSDVVITTYGVLTSEFSQENSADHEG- 661

Query: 597 RRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPI 656
                           +  + W+RI LDE   ++++ +  +  A+ L A  RWC+TGTPI
Sbjct: 662 ----------------IYAVRWFRIVLDEAHTIKNSKSQISLAAAALVADRRWCLTGTPI 721

Query: 657 QRKLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHV 716
           Q  LEDLY LLRF++  P+    WW ++++ P+E  D   ++      K IM R  K   
Sbjct: 722 QNNLEDLYSLLRFLRIEPWGTWAWWNKLVQKPFEEGDERGLKLVQSILKPIMLRRTKSST 736

Query: 717 TDE----LQLPPQEEQVTWLKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDC 776
             E    L LPP + +V + + S  E  FY         + R  ++   D FV++     
Sbjct: 782 DREGRPILVLPPADARVIYCELSESERDFYD------ALFKRSKVK--FDQFVEQ----- 736

Query: 777 VTSDIPSDLLITHADAGKLLSTLLKLRQACCHP 795
                   +L  +A    +L  LL+LRQ C HP
Sbjct: 842 ------GKVLHNYA---SILELLLRLRQCCDHP 736


HSP 2 Score: 104.4 bits (259), Expect = 9.3e-22
Identity = 77/290 (26.55%), Postives = 135/290 (46.55%), Query Frame = 0

Query: 1365 ENTSKADEESCPVCQEKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCR 1424
            E   K ++  CP+C E L +  ++  C H  C +CL A    +  G        +CP CR
Sbjct: 784  EELRKGEQGECPICLEALED-AVLTPCAHRLCRECLLASWRNSTSG--------LCPVCR 843

Query: 1425 QHTDFGNIAYADDSKNETLDPSTSLETSREHEMSITVQGSY--GTKIEAVVRRILWIKYT 1484
                         SK E +   T      E    + V+ ++   +KI A++  +  ++  
Sbjct: 844  NTV----------SKQELITAPT------ESRFQVDVEKNWVESSKITALLEELEGLR-- 903

Query: 1485 DSEAKVLVFSSWNDVLDVLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKR 1544
             S +K ++FS W   LD+L      NN +F+R+ G                   + ++++
Sbjct: 904  SSGSKSILFSQWTAFLDLLQIPLSRNNFSFVRLDG----------------TLSQQQREK 963

Query: 1545 LSTDLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENK 1604
            +  +  E  S  VLL+ ++ G  G+NL  A +  +++P  NPA E QA+ R+HRIGQ  +
Sbjct: 964  VLKEFSEDGSILVLLMSLKAGGVGINLTAASNAFVMDPWWNPAVEEQAVMRIHRIGQTKE 1023

Query: 1605 TFVHRFVVKDTVEESIYKLNRSRESSSFITGNAKNQDQPFLTLKEVESLF 1653
              + RF+VK TVEE +  +   ++    I+G   +Q+     ++E++ LF
Sbjct: 1024 VKIRRFIVKGTVEERMEAVQARKQ--RMISGALTDQEVRSARIEELKMLF 1028

BLAST of CSPI06G02230 vs. TAIR 10
Match: AT5G43530.1 (Helicase protein with RING/U-box domain )

HSP 1 Score: 166.4 bits (420), Expect = 2.0e-40
Identity = 144/523 (27.53%), Postives = 210/523 (40.15%), Query Frame = 0

Query: 292  LRPYQRRAAYWMIQREKGFSGNSGLGENVQLISPLCMALK-CLDTCLRVFYNPFSGNLTL 351
            LRPYQ++A YWM + EKG      + +  + + P   A + C +    ++ N FSG  T+
Sbjct: 616  LRPYQKQALYWMSESEKGID----VEKAAETLHPCWEAYRICDERAPSIYLNIFSGEATI 675

Query: 352  NAEQVLPHVYGGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFK 411
                      GGILAD MGLGKT+  +A IL+       GR   + E             
Sbjct: 676  QFPTATQMARGGILADAMGLGKTVMTIALILAR-----PGRGNPENE------------- 735

Query: 412  RLKRERIECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRR 471
                                       D+  A  +AD                       
Sbjct: 736  ---------------------------DVLVADVNAD----------------------- 795

Query: 472  EKRNNRNTLNVIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTH 531
             KRN +                  E+  A  +  A G TLI+CP  +L QW+ E+  H+ 
Sbjct: 796  -KRNRK------------------EIHMALTTVKAKGGTLIICPMALLSQWKDELETHSK 855

Query: 532  PGSIKLLVYEGVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMR 591
            P ++ +LVY G       G  +     + + D+VLT+Y VL      D            
Sbjct: 856  PDTVSVLVYYG-------GDRTHDAKAIASHDVVLTTYGVLTSAYKQD------------ 915

Query: 592  FQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLE 651
                  +  +   RI W+RI LDE   ++S  T A +    L +  RWC+TGTP+Q KLE
Sbjct: 916  ------MANSIFHRIDWYRIVLDEAHTIKSWKTQAAKATFELSSHCRWCLTGTPLQNKLE 975

Query: 652  DLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDE-- 711
            DLY LL F+   P+    WW ++I+ PYE  DP  ++      + +M R  K     E  
Sbjct: 976  DLYSLLCFLHVEPWCNWAWWSKLIQKPYENGDPRGLKLIKAILRPLMLRRTKETRDKEGS 1000

Query: 712  --LQLPPQEEQVTWLKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDI 771
              L+LPP + QV   + S  E  FY         + R  +Q   D FV +          
Sbjct: 1036 LILELPPTDVQVIECEQSEAERDFY------TALFKRSKVQ--FDQFVAQ---------- 1000

Query: 772  PSDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPM 810
               +L  +A+   +L  LL+LRQ C HP +  S   S Q + +
Sbjct: 1096 -GKVLHNYAN---ILELLLRLRQCCNHPFLVMSRADSQQYADL 1000


HSP 2 Score: 98.6 bits (244), Expect = 5.1e-20
Identity = 83/324 (25.62%), Postives = 139/324 (42.90%), Query Frame = 0

Query: 1335 SKQTIPLDSSSDLALTQEP------AITSTATEQKNENTSKADEESCPVCQEKLNNQKMV 1394
            S+Q   LDS +   L   P      A +    E+  ++    + + CP+C E  ++  ++
Sbjct: 994  SQQYADLDSLARRFLDNNPDSVSQNAPSRAYIEEVIQDLRDGNSKECPICLESADD-PVL 1053

Query: 1395 FQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTS 1454
              C H  C +CL         G        +CP CR              + E +    S
Sbjct: 1054 TPCAHRMCRECLLTSWRSPSCG--------LCPICRTIL----------KRTELI----S 1113

Query: 1455 LETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLHYAFVAN 1514
              T     + +       +K+  +++ +  IK + S  K +VFS W   LD+L       
Sbjct: 1114 CPTDSIFRVDVVKNWKESSKVSELLKCLEKIKKSGSGEKSIVFSQWTSFLDLLEIPLRRR 1173

Query: 1515 NITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLIQHGANGLN 1574
               F+R  G                   +  ++++  +  E +   +LL+ ++ G  GLN
Sbjct: 1174 GFEFLRFDG----------------KLAQKGREKVLKEFNETKQKTILLMSLKAGGVGLN 1233

Query: 1575 LLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFVVKDTVEESIYKLNRSRESS 1634
            L  A  V L++P  NPA E QAI R+HRIGQ+   FV RF+VKDTVEE + ++   ++  
Sbjct: 1234 LTAASSVFLMDPWWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQVQARKQ-- 1276

Query: 1635 SFITGNAKNQDQPFLTLKEVESLF 1653
              I G   +++     L+E++ LF
Sbjct: 1294 RMIAGALTDEEVRSARLEELKMLF 1276

BLAST of CSPI06G02230 vs. TAIR 10
Match: AT3G20010.1 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )

HSP 1 Score: 122.1 bits (305), Expect = 4.3e-27
Identity = 117/482 (24.27%), Postives = 188/482 (39.00%), Query Frame = 0

Query: 361 GGILADEMGLGKTIELLACILSHQ-MSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIEC 420
           GGILAD+ GLGKT+  +A IL  + +S  +       E + LV D   E    K E    
Sbjct: 304 GGILADDQGLGKTVSTIALILKQKIVSQLKSESSCKQETEALVLDADDESDNAKHE---- 363

Query: 421 LCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTL 480
                       G  V+ ++         V  + +  ++ +   ++ +S   ++      
Sbjct: 364 -----------SGSHVKPEL--------KVSSNSETSVLSACGNDENDSSDMEKAEDEEA 423

Query: 481 NVIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEI-LRHTHPGSIKLLV 540
           N   RA +                     TLIVCPA ++ QW  E+  + +    + +LV
Sbjct: 424 NSSTRAFQW--------------KRPAAGTLIVCPASVVRQWARELDEKVSEESKLSVLV 483

Query: 541 YEGVRDTSLLGTLSAQINDLINSDIVLTSYDVL------------KEDLSHDSDRHEGDR 600
           Y G   T          N+L   D+V+T+Y ++             E+   ++DR+    
Sbjct: 484 YHGSNRTK-------DPNELAEYDVVVTTYAIVTNEAPNKFLVDEDENDEKNTDRYGLAS 543

Query: 601 RFMRFQKRYPVIPT----------------------PLTRIFWWRICLDEGQMVESNATA 660
            F   +KR  V+                        PL ++ W+RI LDE Q +++  T 
Sbjct: 544 GFSNNKKRKVVVGASKKSKRRGRKSTNDTSSEPDCGPLGKVGWFRIVLDEAQTIKNYRTQ 603

Query: 661 ATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPG 720
                  L A  RWC++GTPIQ  ++DLY   RF++  P+ V++ +   I+ P  R    
Sbjct: 604 MARSCCTLRAKRRWCLSGTPIQNTIDDLYSYFRFLRYDPYAVYKSFYSTIKVPISRNSCQ 663

Query: 721 AMEFTHKFFKQIMWRSLKIHVTDE---LQLPPQEEQVTWLKFSPIEEHFYQRQHETCVSY 780
             +      + IM R  K  + D    + LPP+   ++ + FS  E  FY++      S 
Sbjct: 664 GYKKLQAVLRAIMLRRTKGTLLDGKPIINLPPKVVNLSQVDFSVAERSFYKKLEADSRSQ 712

Query: 781 AREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL-------LSTLLKLRQACCHP 797
            +                              +ADAG L       L  LL+LRQAC HP
Sbjct: 724 FK-----------------------------AYADAGTLSQNYANILLLLLRLRQACDHP 712


HSP 2 Score: 68.6 bits (166), Expect = 5.7e-11
Identity = 74/333 (22.22%), Postives = 134/333 (40.24%), Query Frame = 0

Query: 1337 QTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEES--CPVCQEKLNNQKMVFQCGHI 1396
            Q +   +S  +    E A+     E ++   ++ +  S  C  C E    + +V  CGHI
Sbjct: 711  QLVKRYNSDPVGKVSEAAVRRLPREARSRLINRLESSSAICYECNEP-PEKPVVTLCGHI 770

Query: 1397 TCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTSLETSRE 1456
             C +C+        +   +       P C+Q     ++ +++ S        +   +S +
Sbjct: 771  FCYECVLEYITGDENTCPV-------PRCKQQL-ARDVVFSESSLRNCTSDDSGCSSSHD 830

Query: 1457 HEMSITV---QGSYGTKIEAVVRRILWIKYTDS--------------------------- 1516
            + +  +V   +    +KI+AV+  +  +   DS                           
Sbjct: 831  NGLDRSVFQKRDFCSSKIKAVLDILQSLSQPDSPNSAQHGQMPSSSRPYDDDDVTIVEPM 890

Query: 1517 ----------EAKVLVFSSWNDVLDVLHYAFVANNITFIRMKGGRKSQTAISEFRGQKIN 1576
                        K ++FS W  +LD++    + + I F R+ G               + 
Sbjct: 891  RLHSSSPSQGAVKTIIFSQWTGMLDLVELRILESGIEFRRLDG------------TMSLA 950

Query: 1577 AKENKKKRLSTDLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRV 1628
            A++   K  S   P+++   V+L+ ++ G  GLN++ A HV+L++   NP  E QAI R 
Sbjct: 951  ARDRAVKEFSKK-PDVK---VMLMSLKAGNLGLNMVAACHVILLDLWWNPTTEDQAIDRA 1010

BLAST of CSPI06G02230 vs. TAIR 10
Match: AT1G11100.1 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )

HSP 1 Score: 119.4 bits (298), Expect = 2.8e-26
Identity = 83/295 (28.14%), Postives = 137/295 (46.44%), Query Frame = 0

Query: 509 TLIVCPAPILFQWQAEILRH-THPGSIKLLVYEGVRDTSLLGTLSAQINDLINSDIVLTS 568
           TLIVCP  ++ QW  E+ +  T    + +LVY G   T          ++L   D+V+T+
Sbjct: 635 TLIVCPTSLMRQWADELRKKVTLEAHLSVLVYHGCSRTK-------DPHELAKYDVVITT 694

Query: 569 YDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATE 628
           Y ++ +    D +  E              +  PL ++ W+R+ LDE Q +++  T A+ 
Sbjct: 695 YSLVSKRKHMDCEPVE-------------FLSGPLAQVSWFRVVLDEAQSIKNYKTQASI 754

Query: 629 MASRLYASHRWCITGTPIQRKLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAME 688
             S L+A  RWC++GTPIQ  + DLY   RF+K  P++ ++ + E I++P         +
Sbjct: 755 ACSGLHAKRRWCLSGTPIQNSIADLYSYFRFLKYDPYSSYQTFCETIKNPISSYPGEGYK 814

Query: 689 FTHKFFKQIMWRSLKIHVTD---ELQLPPQEEQVTWLKFSPIEEHFYQRQHETCVSYARE 748
                 K++M R  K  + D    + LPP+  ++  + F+  E  FY +    C S    
Sbjct: 815 TLQAILKKVMLRRTKDTLLDGKPVISLPPKSIELRRVDFTKEERDFYSKLE--CDS---- 874

Query: 749 VIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKLLSTLLKLRQACCHPQVGSS 800
                +D F +      V  +  + LL+           LL+LRQAC HP + SS
Sbjct: 875 -----RDQFKEYAEAGTVKQNYVNILLM-----------LLRLRQACGHPLLVSS 887


HSP 2 Score: 68.6 bits (166), Expect = 5.7e-11
Identity = 48/168 (28.57%), Postives = 85/168 (50.60%), Query Frame = 0

Query: 1487 KVLVFSSWNDVLDVLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTD 1546
            K +VF+ W  +LD+L     ++ I + R  G               + A++   +  +T 
Sbjct: 1073 KAIVFTQWTKMLDLLEAGLKSSGIQYRRFDG------------KMTVPARDAAVQDFNT- 1132

Query: 1547 LPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVH 1606
            LP++    V+++ ++  + GLN++ A HV++++   NP  E QAI R HRIGQ     V 
Sbjct: 1133 LPDV---SVMIMSLKAASLGLNMVAACHVIMLDLWWNPTTEDQAIDRAHRIGQTRPVKVV 1192

Query: 1607 RFVVKDTVEESIYKLNRSRES--SSFITGNAKNQDQPFLTLKEVESLF 1653
            RF VKDTVE+ I  L + +    +S    +     +  L+++++  LF
Sbjct: 1193 RFTVKDTVEDRILALQQKKRKMVASAFGEHENGSRESHLSVEDLNYLF 1224

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q149N83.7e-14830.90E3 ubiquitin-protein ligase SHPRH OS=Homo sapiens OX=9606 GN=SHPRH PE=1 SV=2[more]
Q7TPQ31.7e-14530.26E3 ubiquitin-protein ligase SHPRH OS=Mus musculus OX=10090 GN=Shprh PE=1 SV=1[more]
Q9UTL94.2e-5923.31Uncharacterized ATP-dependent helicase C144.05 OS=Schizosaccharomyces pombe (str... [more]
Q9FNI67.2e-4327.40DNA repair protein RAD5A OS=Arabidopsis thaliana OX=3702 GN=RAD5A PE=1 SV=1[more]
Q9FIY72.8e-3927.53DNA repair protein RAD5B OS=Arabidopsis thaliana OX=3702 GN=RAD5B PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K8S10.0e+0099.70RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014630 P... [more]
A0A1S3C3N10.0e+0095.57E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A1S3C4210.0e+0093.26E3 ubiquitin-protein ligase SHPRH isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A1S3C4R30.0e+0091.73E3 ubiquitin-protein ligase SHPRH isoform X3 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1FE160.0e+0085.99E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_011656441.10.0e+0099.70E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucumis sativus] >KGN45843.2 hypot... [more]
XP_008456785.10.0e+0095.57PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucumis melo] >XP_00845... [more]
XP_008456789.10.0e+0093.26PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X2 [Cucumis melo][more]
XP_038884903.10.0e+0091.84E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida] >XP_038884904.1... [more]
XP_008456790.10.0e+0091.73PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X3 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT2G40770.10.0e+0056.08zinc ion binding;DNA binding;helicases;ATP binding;nucleic acid binding [more]
AT5G22750.15.1e-4427.40DNA/RNA helicase protein [more]
AT5G43530.12.0e-4027.53Helicase protein with RING/U-box domain [more]
AT3G20010.14.3e-2724.27SNF2 domain-containing protein / helicase domain-containing protein / zinc finge... [more]
AT1G11100.12.8e-2628.14SNF2 domain-containing protein / helicase domain-containing protein / zinc finge... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1533..1553
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR45865E3 UBIQUITIN-PROTEIN LIGASE SHPRH FAMILY MEMBERcoord: 1..1669
NoneNo IPR availableCDDcd18070DEXQc_SHPRHcoord: 292..700
e-value: 1.14861E-88
score: 287.318
NoneNo IPR availableCDDcd18793SF2_C_SNFcoord: 1465..1609
e-value: 3.2097E-32
score: 120.659
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 1365..1428
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 1499..1598
e-value: 0.0076
score: 24.3
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 288..669
e-value: 8.6E-14
score: 61.8
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 1375..1423
e-value: 1.3E-4
score: 31.3
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 1375..1424
score: 11.22052
IPR001965Zinc finger, PHD-typeSMARTSM00249PHD_3coord: 1374..1424
e-value: 29.0
score: 0.6
coord: 418..494
e-value: 3.7E-4
score: 29.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 1441..1689
e-value: 6.0E-38
score: 132.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 273..697
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 708..1662
IPR038718SNF2-like, N-terminal domain superfamilyGENE3D3.40.50.10810coord: 490..723
e-value: 4.1E-31
score: 109.7
IPR038718SNF2-like, N-terminal domain superfamilyGENE3D3.40.50.10810coord: 346..419
e-value: 1.4E-5
score: 25.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 1358..1440
e-value: 2.9E-9
score: 38.0
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 420..480
e-value: 2.0E-8
score: 35.5
IPR000330SNF2, N-terminalPFAMPF00176SNF2_Ncoord: 295..796
e-value: 4.6E-84
score: 282.1
IPR011011Zinc finger, FYVE/PHD-typeSUPERFAMILY57903FYVE/PHD zinc fingercoord: 415..454

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G02230.1CSPI06G02230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005524 ATP binding
molecular_function GO:0140658 ATP-dependent chromatin remodeler activity
molecular_function GO:0046872 metal ion binding