CSPI06G02220 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G02220
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionGPI transamidase component PIG-S
LocationChr6: 1636962 .. 1644955 (-)
RNA-Seq ExpressionCSPI06G02220
SyntenyCSPI06G02220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCGGATATTTAAGCATGTAAATTCATCCATTCACTTACTATATGCGTTTTAACCAAGGTGTGCATGTTAAGTCGCATCGCGTCCGGTCTATGAAAAAACTCCTAATTACGCTAACACTTGGAAGTAGAAATTAGGTGATCTTCGGCTAAGCTTCTATGAATTAGCTTTAGCTGCCTCGGTAACCCAGCCCAACTTCCGTTTGATTCAGCAATGGCGGAAATCTCAGAACCTTCGAAACCTCCACAACTTGACTCCGGATCATCGGAGGCCGGACTTTCACAGTTCGACCCCAAAACCATGCGGAATACTAAGCCTGGATTCAAGCGCCTAATTCTTACTATCTCCGTCTTCTCCTCTTTCCTCTTAGGTCTCACGCTCTCTCTCTCCCTATCTATGGGAACACATGTGATCGTTAATATCTCGTTCACTCTATGATTCATGCGATTTTCTATTGCAGGTCTTCCATTTCTATGGAAATCCGTTGAAATCTACCGCGCGCCACTTCCGTTCAAGGACATCGATGCCCTCTCCTCTCATATAGAATCGTCACCGTTGCAATTTCCCTGTACTTTTCGGGTTATATTTTTTGGATTTGATTCCATGGCTTCTAGAGCCGAGCAGCTAAAATCGTCTATCCTCGATGAGATGACTAAACTTTCTTCTAAATCCTCACTTTGTGGCAGTTGCAGCAACAACTATGCTGTCTCTGTAGTTATAGAATCGGGTTCTGATTGCTCACAGACGCGCACTGACGCATCCTCGTGTTCTTGGCGTTGTGGAGCTCTCAGTGCTTCTGATTTTGCTGCTTCTTTGGAAAATGGGCTTCAAAGTGCCGATGATTTCTTGGAGGTTGCTCTGGGAGGTTGTTCGAAGCCAGCTTCTGGTGGGAGAGTTTATAGTGTAGTGGTTATGAACAAGGGCGAGAATGTGAAGGCTACCATCGGAAAATATCGTCATGGTTGGATTGTTGGGAAGGTTTCAGAGGCGGAAGCCATAGCAAAAGTAGCAGAAACTTTTGTTAAGTTGTTTGGTAATGGTGGAACGGAGGATGGGTTAATTCCCGGGGAGTTTATGCCTGTTGGTGCTGATGGAAAGATTTTTCTTTCTTTTAATTTGTTGAATGCAGATCCAGATGATTGGATTTATGACTGGTATGTTGCTCTCTTTAGTATCATCTTCCTTTGGCCTGAATTCTTGTTGATCATACTGGCATCATGATGTAGAAGTCATTGTCTAGGCCCGTTATGGTGATGATTTTTGGTATAGCTTCAGTGTTGAATAGTATATCTTGATTGGATTAGACCGTATAGAGAATTTAACAATTCTGCCAGTTTTTCTCATGATAAGTGGGGAATATTTCATGCAGATTGCTAGTTTAGTAACTCTTGATTCTCTGGTCATCCTTAGAAACTATAGCTAGAATGATTTTGGATTGACTTCTTGATGCTTGCTGCCCATTCCAATTTTAGAAGCTCTAATAGTTAATTTAAAGCTTTCCAACTAGTCAAGATAAAGAAAGAATGAAGACAATTAGACACATACAGTCTGTCACTCTCAAACCTCACATATCTCCCTTCCTAAATACTTGAAATCATTTCTATTCCTAAATTATCAACAATCCCAATATCAGAGACTGCTGTAACAGCTCTGATGACTACACTGCTTAAGATATTTTTCATTTCTGCCATTGAGAATTATATTCCTAGTTATAGGCAATTTTCGTACATTAGTTTTTGCAGTCTGTCTATTATGAATCTCTGCTATTTCGTAGGGATTTTCAAAAAGTAGATGAAGTTATATTGAAACCTTTAATCGAGGAATTAGCACCTATAGCAAATGTCTCAGTTGAGAGCCAGGTATGATGTTCCTCATGGAAGGCTTATTGTCAGTTCATTCACTCTCCTGTTTTTCAACTAATTCAATATTTTCCAAAATCCTTGAAAATACACGGACAAGGTATTCAGTCTAATCCAATTTCCATTCACTGACCAGGTTTTATATCATACGCCAACCTCCTCCTTTTCTTACTGGGACAACAAGCAGGAAAGCTACATCTTCAACACTAAAGATCTTCCCTTTTTTGTATGGTTCTTTTAGTTTTTAGTTTACTATTCTCTCCCCCACCTTCAAAAGAAAAAAAAGATAGATGCTCGTTGGTGATTTATCCCTCCTTCTATCAGGCTTAACGTTTTCGTTATTCGTATTGAATTTATATCTGTCTCTATTTGTTTACTCACTTTGCCTGTTTCCTGACACACGGTTATGCCATTTTTTTCACACTTTATCCAGTCTAGAGTTGTAGGATTGCGGTCTCATCCTCCATTTTGTTTATTTATTTTAAAGCATCTTCTTATTTTTTTAAATTTAATAGGTGAATTCAAACGAGTGGCATTTGGATACATCAATTGCAGCAGGCGGGAGGTCAAAGATATTGCATTTTGTGGTGTATAACCTTTAACTCATATCACACTTTTTATTCATTTTTAATCAATCTAAGATACCAATTGTGCCAAGCTCTTACACTATAACTATGTTGGGAAGGTTCATTGTCCTTGATTCCCCACTGTTATGCAAACATGATCTATCTGACAATTGGAATCACTTGAGTTTTCTGTTAGGCCTCTGATAGCTAAGTATATATCAGAAGGCTAGAAAGACCGCGAATAAGAATTAGGATGTCTCTCTAATTAGCTATTTATAACTATTAAAGGGGTGTTTGAGGCAAAGAATTGGTTATTATAATCCTAGGTTATTATAGTTGAGTATGAGTTATAATAGTTTGTGTTGAAGTGTAGATTATTTTAGTTTGGGTTATAATAGTACGTGTTAGAGATATAAACTATTTTAGTTTGAGAAGAAAATTGTAAACACCAAATTGGCCTAGGGTTAAAAAAGGAGACAGTCTTAATAACTAACTAAGAGGTTATGGGTTCCACGTTGGCACCCAAATGTTGTAGGTTCAAGCTGGTTGTACCATGAGATGAGCAGATATGCGTAATCTGGCCGAACAGTTACGAATATATAAACAAATAAAAAACATTATAGTAAAGAATAAAAAATGATAAATGTGGAATAGTAAAACTGTAGCAAGTAGGAGTTTTGAAATAGTGTTTGACTGTAGTTAATTGGGGATTACAAAATAGTACTTATTATAACAAAGAGTTGATTATTATAGCATTAGATAGTCCTTGTCCCAAACAATCCCCAACTGTTTTTAGATCCTTATGTGTTAATTGTATCTTTGTTACTTTAGATGAGTATGAGGATGTTTAGTTAAGTAGCAGTTGGTAGTTTGGGCAAAGATGAAAATTTGTGATATTTCATCAGAATATTGCCGAAATGTCGTCGACATTTCCAATATTTTCGCATCGTTTATCAAATATAAAGCCTATTCTTCACAACTGCTCAATAAAGTGTGTTGGTTGTCTGGTTGCTGTATGGTTAAGCAGTTATACATGAGCGTGCAAGTCCAACACCCACCTGTGTAAAATTTTTAGCGTTTTTGTTTCATTACTTTTTTTTTCTTTTTTCTTCTTTTTTCTTCTTTTAGATCTCCCAATTTTTTTATTTTTATTTTTATAATTTAGGGTCTTTTTCTCTCTCATTCACACTTAGTGATTTTTCTTAATTCTTTCACTATATACTTCTTTTAATTTTTTATACTGTATAATTTATATTTTCCGTTGTTTTTTCTCTTCAATAGTCCATAATCTCAATCCTCTCTTTACCAATTGCCTCATTTTTTTAACTTTTTCAATTTTTTTATTCTGAAATTTGATTTTACTTCATATATTAGAAATACGTACTTTTTAGGAAAATATTGGTTGTAAACTAGAACAATTTAAATCAGTCTACTTGATTGTAAACTAGATCACTAATTCACTGATTGTAAACTCCTGAACTAAATATTCAGTTCATAACCATTTTTCAGTTTTCACTATTATCTATTATTTCATTACCAATCATATTTGGCTTATAATTATTGATTTATATACTTAAAGTCATATTTTATTACTTAAATAAGTTTTCTCTAAAATTTTACAATATTTTTATAAATTTTCTGTGATATCCGTGTCTTTTCCCAGAATATCCATCGATAGATTCGTAAATCTGAGTTACCGATATTTACATTGCTAACAATATTATCGTCCTTTGTTTTGGGAGAGACAATAGCCCTCTTAGCTTGGTTATCAATGAGTAGCTTGTAGTTTATTCTTTAAAGCAATTTCTACCTTTTGAAGATAACTATCATTTTCTTACGGTGATGTCTATTCTGATTGATTTGAATTGAAAGAAGAGGTTAGTGGCCATTATGTTTAAACATAAAGTTCAGTTAAGGATAAGATGTGTAATGAAGGAACCTAGGAGTATTTTCCATTTATGTGTTGAAAAACTGCTAATCTGTTTCTGCTCATTTATATTATTACTCCTAATCAAGTTTTGTTTCATTAGCCTTTTTTTTTGTTGTTGTCAGATATATACCATCAGCAAGGGAGTGCCCTCTTCTTTTACAGCTTCCTGATGGCCAGATTTCAGAAACAAATGGGTTTATATCTCCTGTATGACAATCTTTTTGCTGTTTACATATATGATTTTTGGGTCAAACTTCTCAAAATCAGGTACAAATTAATCTTGCAGACATGGGGAGGCGTAATTGTTTGGAACCCTAAAGGCTGCCTGAGAGACCATGAAAGTAAGCTGCTCCATAGGCATATGATTTTGTATCCGGTAAGATGCTGATGTTATGTACCAAACTTCGGGTATTCTATCTAATTTTCTGATGATTGATCCTATATACTGGAACAGGAGCTCGAGAAAATTGTTGAAGTATTCCTGGGGCAATTTCGGCAACTTTTTGGTCTAAAGTCCAATCCCCAACATGTTGGTCTGTCGGGCACATTCAACATTTTAACCAGTCAGAAAGGCTTTACAGAATGGTTAGGAAAATATGTGTTTTTTCCCTTTTATGTTTCAAGATTAAGTTTTAGATTCATTACCAGCTGTCAAAACTTATCTCACTAATTCCATTATTCTAGCCCTTACTAGCGATATCCTGTTAATTGATAGCATATTTATGTTGCAAACAAACTATATTTTGTTAACAGAAATTCAAGTTTTTTCAATGCACACCCACTTGTCTCATGAAGCTCCTTCGTTCTTTTGAGCCTTGCAATGTCTGCAATAATCTTTATGTCAAATATATGTATTTATATGATTGACCCATCTGTAGGGAAATGGATTTTCTATCGAGGCAGCATTCATGTTTTAATCTTCATTCATGTGCTTCATCCCTCGGATCTCTTTCTAGATTGGTATGTACTTGATTCTTGGTTGAGTTCATAGTAGGATCCGCAGTGTGAAAATTGTTCTTCTTGGTCCCTTTATTTGTTTACTTATTTTTTTCGAACTTTGATTACTTTGTTGTGCAGGTTCAATCACTGCCGAGGATGATTATCATTGATGAGATTGGAAAACAGGTAATCTATTGCTTGTCTGTAAATCTTTTTCTACATTTCTTTTCTGGATTGATTGGCGTACTTACGTTAGATTCTTTTGCTAATCGCCAAATGCTTGAGCTTTGTATGAAATTTCCAAACTTTGTTGGTATGATGTTATTCTAAGTAGTGACCTTCTTCAAGGTTCCTCCAAACTTATTTCTTAAATGGTTTCTAGTAGTGTTTTTCAGCCTGAATGTTATTCTTTCTCTCCTCTGAAACCTCGTCAGCTCTGTCAAGCTTTATAGTCAATAATGGGTTATTGCCAGACAAGAGAATATTTTCTATCTGGTCATTTAAAACTTAAACCTTGCATTTTCTTCAGTCCTCGGACAATAACCAAACAGGGGAGAAAACCAAATCACATCTCTTTCTTTCCGTGTGTGGGTAGGTGGGGGAATACATAACCAAGTAACAAAAGTTTCTGCTTTATTTGTCAAAAGGACCACTGGATTCTCTTTTCCCTCGAAAAAGATCTTGTCTACAGCAAAATCATCCATGTTTGTGATGGATCCAACACCTCTCTTTTGGAAGGCCTATGGATTTGTAATGAACTTAAGAACATACCTAAAAATGAGGCCATCATTTGAGAGACATGGTCAAGCCATTGGGATCATTGGCCAATTGGACGTTTATAGGAAATCAGCTGCACACAGAGATCCAAGTGTGAGCTGCATCAACTCAGATTTTCTCAACCTTTTCGCTTCACTCAAGCAAAGACTAATGCCTTTGGAAATTGCGTTACTCTGCCAAATGTCTCACAAAATATCTCATGGCTCCGATCCAAAATTTCAGTACCTTCTCATTCAAAAATATATGGAAGGTCAAACCCCTGAAAGTTTGACTTTTTCTTTTGGAGCTTGCCCAGTGCCCATTCTTGCTTGAATAACCACGTCAAACTTCAAAAGGAACACCATGGATGGCTTTCTCCTGAAGTCGGTGCTCACTAAAACATACAGCATTTTTCACATGCCTATTTTCTATGATCCTTTTTGGATCATGTCCTTACTGGTTGACTGCCGCACAACGTGCTATAATTTATCCTTGAAGGACATCCTACATTTGAAATGAGAGAGCTGTATATTCAATCAAAAAAATGAAAACTTGGTGCCTCAATTATAAACATGGTTTCTCTTTTATTTAATTGGAGGTTTTGAATACAGGCGTGGCCCTTTTTATTTACTTGTATTCCATGGTGTATAATATGAGGTCTTAATTTTTTGTTGTCTTGATCAGGTAAAATATTCTCTCGAGGCTGCAAATTTGGCCCAGAAAAATGCCTCTATGGGAGTTTTTGATGCAGCAGCCAGTATGATCCTTCTCTCATTACTATATGGTCATTGATTTGTTGACTGTAATTCTGATTGAATTAATTAAAATTCTGAACTGAGTTCCTCTTCCTTATCAGTATCTTCCAGGCAAGCAAGGTCACTAGCAGAAGATGCATTTTTTCACCCATCAATAATGTCAGTCAGCTATTTTTCATTTGAGCACTGCTTTGCTGTCTACTCGGTATGATTATTGGTTCCTACACTTATGTTTATAGTATCTTTGTATTTATAATTCATGTTGGATTAAATTATAAATTTAGTCTACTAGTTTGAGCATAATATCATCGGGTTTTTTCAACTTAACAGATTCCGATTGCATCTCTAATCTTTTACTTTCTATTTAATATCTTGACTCTTTACATTAACACTAAACTTGAATGTTGACATGACAAGCTCATAGTTTGTCATGAATTCTAAGAAATATCTGGTAACATGATTACTAGAATTTTATCATGACGTTTCTCATGGTTTGACACTTTGTTTAGTTGTCACTTAGTCACATCTTGATTCAGGCTATAAAATGACATTGCAACCTTCTGTTAATAGTGTTGATGGTATCAACTAAGTTGGAATATAGTTTAAAGTTTAGGGATATGATTAAAATCTATTAAATTAAGGCGAAATATATATAATATAGCAGTAGAAAAGAAAGGTTGTATGGTTTCATAAACACTAATATCTGTGCAACATTATCATTGATATAGAAAAATTAAATTTCGAACATAGGTGACACATTTTTGGGATTACAAAGAAAATGGCTTTAGCTTTATAGACGGCATTTTGAAACCCCATTGTTATGTTTGTCTTTCTTTGGACAGCCTTTCTTCTTGCCAGTTGCTTTGCACGTAATTTTGGCAGCAGTGAGAGAGTGGAAAAGATACAAGCAAGAACACAAGAAGTATATAGCTTTTTTGGCCAAACCAAAGCAAAGCTAGCAACTTAATCATGGTGAATTTGTGTCGGCTTAGTCAGTTCTTACTTTTTCTTTTTCCATTTTAGGTTGTCCTTGTAACTTGAGAATGCTTCCCAACAATGTATTCTTATATATATATATTGAATGTTAGTTTAGGGTTCTAAACAAGTAGATATCTAATATTTGTGGCATTTTTAACATAAATTGATTCATTTATGAAAGTTTAGGATTTAAATTTATACTATTATTCAACT

mRNA sequence

CTCGGATATTTAAGCATGTAAATTCATCCATTCACTTACTATATGCGTTTTAACCAAGGTGTGCATGTTAAGTCGCATCGCGTCCGGTCTATGAAAAAACTCCTAATTACGCTAACACTTGGAAGTAGAAATTAGGTGATCTTCGGCTAAGCTTCTATGAATTAGCTTTAGCTGCCTCGGTAACCCAGCCCAACTTCCGTTTGATTCAGCAATGGCGGAAATCTCAGAACCTTCGAAACCTCCACAACTTGACTCCGGATCATCGGAGGCCGGACTTTCACAGTTCGACCCCAAAACCATGCGGAATACTAAGCCTGGATTCAAGCGCCTAATTCTTACTATCTCCGTCTTCTCCTCTTTCCTCTTAGGTCTTCCATTTCTATGGAAATCCGTTGAAATCTACCGCGCGCCACTTCCGTTCAAGGACATCGATGCCCTCTCCTCTCATATAGAATCGTCACCGTTGCAATTTCCCTGTACTTTTCGGGTTATATTTTTTGGATTTGATTCCATGGCTTCTAGAGCCGAGCAGCTAAAATCGTCTATCCTCGATGAGATGACTAAACTTTCTTCTAAATCCTCACTTTGTGGCAGTTGCAGCAACAACTATGCTGTCTCTGTAGTTATAGAATCGGGTTCTGATTGCTCACAGACGCGCACTGACGCATCCTCGTGTTCTTGGCGTTGTGGAGCTCTCAGTGCTTCTGATTTTGCTGCTTCTTTGGAAAATGGGCTTCAAAGTGCCGATGATTTCTTGGAGGTTGCTCTGGGAGGTTGTTCGAAGCCAGCTTCTGGTGGGAGAGTTTATAGTGTAGTGGTTATGAACAAGGGCGAGAATGTGAAGGCTACCATCGGAAAATATCGTCATGGTTGGATTGTTGGGAAGGTTTCAGAGGCGGAAGCCATAGCAAAAGTAGCAGAAACTTTTGTTAAGTTGTTTGGTAATGGTGGAACGGAGGATGGGTTAATTCCCGGGGAGTTTATGCCTGTTGGTGCTGATGGAAAGATTTTTCTTTCTTTTAATTTGTTGAATGCAGATCCAGATGATTGGATTTATGACTGGGATTTTCAAAAAGTAGATGAAGTTATATTGAAACCTTTAATCGAGGAATTAGCACCTATAGCAAATGTCTCAGTTGAGAGCCAGGTTTTATATCATACGCCAACCTCCTCCTTTTCTTACTGGGACAACAAGCAGGAAAGCTACATCTTCAACACTAAAGATCTTCCCTTTTTTGTGAATTCAAACGAGTGGCATTTGGATACATCAATTGCAGCAGGCGGGAGGTCAAAGATATTGCATTTTGTGGTATATATACCATCAGCAAGGGAGTGCCCTCTTCTTTTACAGCTTCCTGATGGCCAGATTTCAGAAACAAATGGGTTTATATCTCCTACATGGGGAGGCGTAATTGTTTGGAACCCTAAAGGCTGCCTGAGAGACCATGAAAGTAAGCTGCTCCATAGGCATATGATTTTGTATCCGGAGCTCGAGAAAATTGTTGAAGTATTCCTGGGGCAATTTCGGCAACTTTTTGGTCTAAAGTCCAATCCCCAACATGTTGGTCTGTCGGGCACATTCAACATTTTAACCAGTCAGAAAGGCTTTACAGAATGGGAAATGGATTTTCTATCGAGGCAGCATTCATGTTTTAATCTTCATTCATGTGCTTCATCCCTCGGATCTCTTTCTAGATTGGTTCAATCACTGCCGAGGATGATTATCATTGATGAGATTGGAAAACAGGTAAAATATTCTCTCGAGGCTGCAAATTTGGCCCAGAAAAATGCCTCTATGGGAGTTTTTGATGCAGCAGCCATATCTTCCAGGCAAGCAAGGTCACTAGCAGAAGATGCATTTTTTCACCCATCAATAATGTCAGTCAGCTATTTTTCATTTGAGCACTGCTTTGCTGTCTACTCGCCTTTCTTCTTGCCAGTTGCTTTGCACGTAATTTTGGCAGCAGTGAGAGAGTGGAAAAGATACAAGCAAGAACACAAGAAGTATATAGCTTTTTTGGCCAAACCAAAGCAAAGCTAGCAACTTAATCATGGTGAATTTGTGTCGGCTTAGTCAGTTCTTACTTTTTCTTTTTCCATTTTAGGTTGTCCTTGTAACTTGAGAATGCTTCCCAACAATGTATTCTTATATATATATATTGAATGTTAGTTTAGGGTTCTAAACAAGTAGATATCTAATATTTGTGGCATTTTTAACATAAATTGATTCATTTATGAAAGTTTAGGATTTAAATTTATACTATTATTCAACT

Coding sequence (CDS)

ATGGCGGAAATCTCAGAACCTTCGAAACCTCCACAACTTGACTCCGGATCATCGGAGGCCGGACTTTCACAGTTCGACCCCAAAACCATGCGGAATACTAAGCCTGGATTCAAGCGCCTAATTCTTACTATCTCCGTCTTCTCCTCTTTCCTCTTAGGTCTTCCATTTCTATGGAAATCCGTTGAAATCTACCGCGCGCCACTTCCGTTCAAGGACATCGATGCCCTCTCCTCTCATATAGAATCGTCACCGTTGCAATTTCCCTGTACTTTTCGGGTTATATTTTTTGGATTTGATTCCATGGCTTCTAGAGCCGAGCAGCTAAAATCGTCTATCCTCGATGAGATGACTAAACTTTCTTCTAAATCCTCACTTTGTGGCAGTTGCAGCAACAACTATGCTGTCTCTGTAGTTATAGAATCGGGTTCTGATTGCTCACAGACGCGCACTGACGCATCCTCGTGTTCTTGGCGTTGTGGAGCTCTCAGTGCTTCTGATTTTGCTGCTTCTTTGGAAAATGGGCTTCAAAGTGCCGATGATTTCTTGGAGGTTGCTCTGGGAGGTTGTTCGAAGCCAGCTTCTGGTGGGAGAGTTTATAGTGTAGTGGTTATGAACAAGGGCGAGAATGTGAAGGCTACCATCGGAAAATATCGTCATGGTTGGATTGTTGGGAAGGTTTCAGAGGCGGAAGCCATAGCAAAAGTAGCAGAAACTTTTGTTAAGTTGTTTGGTAATGGTGGAACGGAGGATGGGTTAATTCCCGGGGAGTTTATGCCTGTTGGTGCTGATGGAAAGATTTTTCTTTCTTTTAATTTGTTGAATGCAGATCCAGATGATTGGATTTATGACTGGGATTTTCAAAAAGTAGATGAAGTTATATTGAAACCTTTAATCGAGGAATTAGCACCTATAGCAAATGTCTCAGTTGAGAGCCAGGTTTTATATCATACGCCAACCTCCTCCTTTTCTTACTGGGACAACAAGCAGGAAAGCTACATCTTCAACACTAAAGATCTTCCCTTTTTTGTGAATTCAAACGAGTGGCATTTGGATACATCAATTGCAGCAGGCGGGAGGTCAAAGATATTGCATTTTGTGGTATATATACCATCAGCAAGGGAGTGCCCTCTTCTTTTACAGCTTCCTGATGGCCAGATTTCAGAAACAAATGGGTTTATATCTCCTACATGGGGAGGCGTAATTGTTTGGAACCCTAAAGGCTGCCTGAGAGACCATGAAAGTAAGCTGCTCCATAGGCATATGATTTTGTATCCGGAGCTCGAGAAAATTGTTGAAGTATTCCTGGGGCAATTTCGGCAACTTTTTGGTCTAAAGTCCAATCCCCAACATGTTGGTCTGTCGGGCACATTCAACATTTTAACCAGTCAGAAAGGCTTTACAGAATGGGAAATGGATTTTCTATCGAGGCAGCATTCATGTTTTAATCTTCATTCATGTGCTTCATCCCTCGGATCTCTTTCTAGATTGGTTCAATCACTGCCGAGGATGATTATCATTGATGAGATTGGAAAACAGGTAAAATATTCTCTCGAGGCTGCAAATTTGGCCCAGAAAAATGCCTCTATGGGAGTTTTTGATGCAGCAGCCATATCTTCCAGGCAAGCAAGGTCACTAGCAGAAGATGCATTTTTTCACCCATCAATAATGTCAGTCAGCTATTTTTCATTTGAGCACTGCTTTGCTGTCTACTCGCCTTTCTTCTTGCCAGTTGCTTTGCACGTAATTTTGGCAGCAGTGAGAGAGTGGAAAAGATACAAGCAAGAACACAAGAAGTATATAGCTTTTTTGGCCAAACCAAAGCAAAGCTAG

Protein sequence

MAEISEPSKPPQLDSGSSEAGLSQFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKSVEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTKLSSKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQSADDFLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGKVSEAEAIAKVAETFVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEELAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRHMILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKKYIAFLAKPKQS*
Homology
BLAST of CSPI06G02220 vs. ExPASy Swiss-Prot
Match: Q6PD26 (GPI transamidase component PIG-S OS=Mus musculus OX=10090 GN=Pigs PE=1 SV=3)

HSP 1 Score: 179.5 bits (454), Expect = 1.2e-43
Identity = 151/554 (27.26%), Postives = 259/554 (46.75%), Query Frame = 0

Query: 49  SFLLGLPFLWKSVEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQL 108
           + LLGLP  WK+ E YRAPLP+ DI  L++ +    L+      V+F          E+L
Sbjct: 27  AILLGLPLWWKTTETYRAPLPYSDISGLNALL----LRLMVPVTVVFTRDSVPLDDQEKL 86

Query: 109 KSSILDEMTKLSSKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFA 168
             +++ E  ++  K  +   C    A    +E          +A S        S  +  
Sbjct: 87  PFTVVHE-REIPLKYKMKIKCRFQKAYRRALEH-------EEEALSLG------SVHEAE 146

Query: 169 ASLENGLQSADDFLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGK--V 228
           A L    + A+  L V +           + S +   +   V+  I +     I+G+  V
Sbjct: 147 AMLAEPEKQAEGSLTVYVISEHSSLLPQDMMSYIGPERTAVVRGLIHREAFN-IIGRRIV 206

Query: 229 SEAEAIAKVAETFVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDF 288
             A+A++   +       +   ED     +  P+ +     ++F+LLN DP      WD 
Sbjct: 207 QVAQAMSLTEDVLAAALADHLPEDKWSSDKRRPLKSSLGYEITFSLLNPDPKSHDVHWDI 266

Query: 289 QKVDEVILKPLIEELAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSN 348
           +   +  ++P +  L+   N SV+SQ+LY+        +D    SY      LP  +N  
Sbjct: 267 EGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASSSYSLAMHSLPHVINPV 326

Query: 349 EWHLDTSIAAGGRSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPK 408
           E  L +S  A     +LHF++Y+P     PL +Q  DG    TN F SP WGG++V+N  
Sbjct: 327 ESRLGSS--AASLYPVLHFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVD 386

Query: 409 GCLRDHESKLLHRHMILYPELEKIVEVFLGQFRQLFGLKSN--PQHVGLSGTFNILTSQK 468
             +  + S+L  R  +   ++ +++EVFL Q R LFG+     P    LSG        +
Sbjct: 387 PKIY-NASELPVRVEV---DMVRVMEVFLAQLRLLFGIAQPQVPPKCLLSG-----PKSE 446

Query: 469 GFTEWEMDFLSRQHSCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQ 528
           G   WE+D L    S  NL +  ++L SL++L+  +  ++I D++  +V  ++ A   A 
Sbjct: 447 GLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAA 506

Query: 529 KNASMGVFDAAAISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILA 588
           K  ++G   +A  +S++A + +E AFF PS++ + YF  +  FA+Y P FLP+A+ ++L+
Sbjct: 507 KALALGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLS 550

Query: 589 AVREWKRYKQEHKK 599
            V+ ++  ++  KK
Sbjct: 567 LVKIFQETRKSWKK 550

BLAST of CSPI06G02220 vs. ExPASy Swiss-Prot
Match: Q3SZL5 (GPI transamidase component PIG-S OS=Bos taurus OX=9913 GN=PIGS PE=2 SV=3)

HSP 1 Score: 167.2 bits (422), Expect = 5.9e-40
Identity = 153/566 (27.03%), Postives = 255/566 (45.05%), Query Frame = 0

Query: 51  LLGLPFLWKSVEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQLKS 110
           +LGLP  WK+ E YRAPLP+  I  L+    S  L+      V+F          E+L  
Sbjct: 29  VLGLPLWWKTTETYRAPLPYSQISGLN----SLKLRLMVPVTVVFTQESVPLDDQEKLPF 88

Query: 111 SILDEMTKLSSKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAAS 170
           +++ E  ++  K  L   C    A                          AL   + A S
Sbjct: 89  TVVHE-REIPLKYKLKIKCRFQKAYR-----------------------RALDHEEAALS 148

Query: 171 LENGLQSADDFLEVALGGCSKPASGGRVYSVVVMNKG---ENVKATIGKYRHGW------ 230
           L N +Q A    E  L   S+ A G     V+        +++ + IG  R         
Sbjct: 149 LGN-IQEA----EAMLAEPSEQAEGSLTVYVISERCSLLPQDMMSYIGPKRMAVVRGITH 208

Query: 231 -----IVGK--VSEAEAIAKVAETFVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLN 290
                I+G+  +   +A++   +       +   ED     +  P+ +     ++F+LLN
Sbjct: 209 REAFNIIGRRIIQVVQAMSLTEDVLAAALADHLPEDKWSSDKRRPLKSSLGYEITFSLLN 268

Query: 291 ADPDDWIYDWDFQKVDEVILKPLIEELAPIANVSVESQVLYHTPTSSFSYWDNKQESYIF 350
            DP      WD +      ++P +  L+   N SV+SQ+LY+        +D+   SY  
Sbjct: 269 PDPKSHDVHWDIEGAVRRYVQPFLSALSAAGNFSVDSQILYYAVLGVNPRFDSASSSYYL 328

Query: 351 NTKDLPFFVNSNEWHLDTSIAAGGRSKILHFVVYIPSARECPLLLQLPDGQISETNGFIS 410
               LP  +N  E  L +S  A     +L+F++Y+P     PL +Q  DG    TN F S
Sbjct: 329 AAHSLPHVINPVESRLGSS--AASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHS 388

Query: 411 PTWGGVIVWN--PKGCLRDHESKLLHRHMILYPELEKIVEVFLGQFRQLFGLKSNPQHVG 470
           P WGG++V+N  PK     + S+L  R  +   ++ +++EVFL Q R LFG+ + PQ   
Sbjct: 389 PRWGGIMVYNVDPKAY---NGSQLPVRVEV---DMMRVMEVFLAQLRLLFGI-AQPQLPP 448

Query: 471 LSGTFNILTSQKGFTEWEMDFLSRQHSCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQ 530
               F      +G   WE+D L    S  NL +  ++L SL++L+  +  ++I D++  +
Sbjct: 449 KCLFFG--PKSEGIMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASE 508

Query: 531 VKYSLEAANLAQKNASMGVFDAAAISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSP 590
           V  ++ A   A +  S G   +A  +S++A + +E AFF PS++ + YF  +  FA+Y P
Sbjct: 509 VYRAVAAVQKAAEELSSGHLASAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIP 550

Query: 591 FFLPVALHVILAAVREWKRYKQEHKK 599
            FLP+A+ ++L+  + +   ++  KK
Sbjct: 569 LFLPMAVPILLSLFKIFLETRKSWKK 550

BLAST of CSPI06G02220 vs. ExPASy Swiss-Prot
Match: Q96S52 (GPI transamidase component PIG-S OS=Homo sapiens OX=9606 GN=PIGS PE=1 SV=3)

HSP 1 Score: 165.6 bits (418), Expect = 1.7e-39
Identity = 139/557 (24.96%), Postives = 256/557 (45.96%), Query Frame = 0

Query: 49  SFLLGLPFLWKSVEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQL 108
           + +LGLP  WK+ E YRA LP+  I  L+    +  L+      V+F          E+L
Sbjct: 27  AIVLGLPLWWKTTETYRASLPYSQISGLN----ALQLRLMVPVTVVFTRESVPLDDQEKL 86

Query: 109 KSSILDEMTKLSSKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFA 168
             +++ E  ++  K  +   C    A    ++   +   +              S  +  
Sbjct: 87  PFTVVHE-REIPLKYKMKIKCRFQKAYRRALDHEEEALSSG-------------SVQEAE 146

Query: 169 ASLENGLQSADDFLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGK--V 228
           A L+   + A+  L V +           + S +   +   V+  + +     I+G+  V
Sbjct: 147 AMLDEPQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFN-IIGRRIV 206

Query: 229 SEAEAIAKVAETFVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDF 288
             A+A++   +       +   ED     +  P+ +     ++F+LLN DP      WD 
Sbjct: 207 QVAQAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKSHDVYWDI 266

Query: 289 QKVDEVILKPLIEELAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSN 348
           +      ++P +  L    N SV+SQ+LY+        +D+   SY  +   LP  +N  
Sbjct: 267 EGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVINPV 326

Query: 349 EWHLDTSIAAGGRSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPK 408
           E  L +S  A     +L+F++Y+P     PL +Q  DG    TN F SP WGG++V+N  
Sbjct: 327 ESRLGSS--AASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNV- 386

Query: 409 GCLRDHESKLLHRHMI---LYPELEKIVEVFLGQFRQLFGLKSN--PQHVGLSGTFNILT 468
                 +SK  +  ++   +  ++ +++EVFL Q R LFG+     P    LSG      
Sbjct: 387 ------DSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSG-----P 446

Query: 469 SQKGFTEWEMDFLSRQHSCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAAN 528
           + +G   WE+D L    S  NL +  ++L SL++L+  +  ++I D++  +V  ++ A  
Sbjct: 447 TSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQ 506

Query: 529 LAQKNASMGVFDAAAISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHV 588
            + +  + G   +A ++S++A + +E AFF PS++ + YF  +  FA+Y P FLP+A+ +
Sbjct: 507 KSAEELASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPI 550

Query: 589 ILAAVREWKRYKQEHKK 599
           +L+ V+ +   ++  +K
Sbjct: 567 LLSLVKIFLETRKSWRK 550

BLAST of CSPI06G02220 vs. ExPASy Swiss-Prot
Match: Q5XI31 (GPI transamidase component PIG-S OS=Rattus norvegicus OX=10116 GN=Pigs PE=2 SV=3)

HSP 1 Score: 161.8 bits (408), Expect = 2.5e-38
Identity = 111/382 (29.06%), Postives = 195/382 (51.05%), Query Frame = 0

Query: 222 IVGK--VSEAEAIAKVAETFVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDD 281
           I+G+  +  A+A++   +       +   ED     +  P+ +     ++F+LLN DP  
Sbjct: 181 IIGRRIIHVAQAMSLTEDVLAAALADHLPEDKWSSDKRRPLKSSLGYEITFSLLNPDPKS 240

Query: 282 WIYDWDFQKVDEVILKPLIEELAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDL 341
               WD ++  +  ++P +  L+   N SV+SQ+LY+        +D    SY      L
Sbjct: 241 HDVHWDIEEGVQRYVQPFLNRLSAAGNFSVDSQILYYAMLGVNPRFDPASSSYSLAMHSL 300

Query: 342 PFFVNSNEWHLDTSIAAGGRSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGG 401
           P  +N  E  L +S  A     +L+F++Y+P     PL +Q  DG    TN F SP WGG
Sbjct: 301 PHVINPVESRLGSS--AASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGG 360

Query: 402 VIVWNPKGCLRDHESKLLHRHMILYPELEKIVEVFLGQFRQLFGLKSN--PQHVGLSGTF 461
           ++V+N    +  + S+L  R  +   ++ +++EVFL Q R LFG+     P    LSG  
Sbjct: 361 IMVYNVDPKIY-NASELPVRVEV---DMAQVMEVFLAQLRLLFGIAQPQVPPKCLLSG-- 420

Query: 462 NILTSQKGFTEWEMDFLSRQHSCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSL 521
                 +G   WE+D L    S  NL +  ++L SL++L+  +  ++I D++  +V  ++
Sbjct: 421 ---PKSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAV 480

Query: 522 EAANLAQKNASMGVFDAAAISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPV 581
            A   A +  ++G   +A  +S++A + +E AFF PS++ + YF  +  FA+Y P FLP+
Sbjct: 481 AAVQKAAEALALGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPM 540

Query: 582 ALHVILAAVREWKRYKQEHKKY 600
           A+ ++L+ V   K + + HK +
Sbjct: 541 AVPILLSLV---KIFLETHKSW 548

BLAST of CSPI06G02220 vs. ExPASy Swiss-Prot
Match: Q10351 (GPI transamidase component PIG-S homolog OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpi17 PE=3 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 1.3e-26
Identity = 97/327 (29.66%), Postives = 156/327 (47.71%), Query Frame = 0

Query: 283 DWDFQKVDEVILKPLIEELAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTK--DLP 342
           +WD +   +    PLIE+LA +A +++E+Q+ Y    +     D+K     F TK  DLP
Sbjct: 257 NWDIENAIQKYFNPLIEQLASLAKLNIETQIQYFVEDAEAYIKDDK-----FCTKHADLP 316

Query: 343 FFVNSNEWHLDTSIAAGGRSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGV 402
             VN+ E +L  S +   R   +HFV+Y+PS +  PL L+  D  I  TN  + P WG +
Sbjct: 317 NLVNNFEKYL--SFSPHIREPTIHFVLYVPSPQIQPLWLENEDSNIIPTNSMLLPQWGSI 376

Query: 403 IVWNPKGCLRDHESKLLHRHMILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNIL 462
              N        E KLLH       +L+    V       L G+   P    LS T    
Sbjct: 377 TTIN----FNVTEKKLLHD-----VDLKDYFRVISRDLLLLLGINDVPVS-SLSATL--- 436

Query: 463 TSQKGFTEWEMDFLSRQHSCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAA 522
                      D L RQ    +    + +L +L++LV S+  M +  EI   VK +L + 
Sbjct: 437 ----------ADRLLRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSL 496

Query: 523 NLAQKNASMGVFDAAAISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALH 582
           ++A K  S    + A   S  A S +++A FHPS+++  YF  E  + +Y+P F P+ + 
Sbjct: 497 DMAYKALSQNNLNEALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIYAPLFAPILIP 553

Query: 583 VILAAVREWKRYKQEHKKY-IAFLAKP 607
           ++++ ++E K   +E K + +A + KP
Sbjct: 557 LLISFIKEVKDMLRERKLHRVANVPKP 553

BLAST of CSPI06G02220 vs. ExPASy TrEMBL
Match: A0A0A0KBT3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014620 PE=3 SV=1)

HSP 1 Score: 1210.3 bits (3130), Expect = 0.0e+00
Identity = 606/609 (99.51%), Postives = 607/609 (99.67%), Query Frame = 0

Query: 1   MAEISEPSKPPQLDSGSSEAGLSQFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKS 60
           MAEISEPSKPPQLDSGSSEAGLSQFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKS
Sbjct: 1   MAEISEPSKPPQLDSGSSEAGLSQFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKS 60

Query: 61  VEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTKLS 120
           VEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTKLS
Sbjct: 61  VEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTKLS 120

Query: 121 SKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQSADD 180
           SKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQSADD
Sbjct: 121 SKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQSADD 180

Query: 181 FLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGKVSEAEAIAKVAETFV 240
           FLEVALGGC KPASGGRVYSVVVMNKGENVKATIGKYRHGWIVG+VSEAEAIAKVAETFV
Sbjct: 181 FLEVALGGCYKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGRVSEAEAIAKVAETFV 240

Query: 241 KLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEE 300
           KLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEE
Sbjct: 241 KLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEE 300

Query: 301 LAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRS 360
           L PIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRS
Sbjct: 301 LTPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRS 360

Query: 361 KILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRH 420
           KILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRH
Sbjct: 361 KILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRH 420

Query: 421 MILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSC 480
           MILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSC
Sbjct: 421 MILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSC 480

Query: 481 FNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSR 540
           FNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSR
Sbjct: 481 FNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSR 540

Query: 541 QARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKKYI 600
           QARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKKYI
Sbjct: 541 QARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKKYI 600

Query: 601 AFLAKPKQS 610
           AFLAKPKQS
Sbjct: 601 AFLAKPKQS 609

BLAST of CSPI06G02220 vs. ExPASy TrEMBL
Match: A0A1S3C4Q8 (GPI transamidase component PIG-S OS=Cucumis melo OX=3656 GN=LOC103496630 PE=3 SV=1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 583/609 (95.73%), Postives = 595/609 (97.70%), Query Frame = 0

Query: 1   MAEISEPSKPPQLDSGSSEAGLSQFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKS 60
           M EISEPS+PPQLDSGSSEAGLSQFDPKTMRNTKPG KRLILTISVFSSFLLGLPFLWK+
Sbjct: 1   MGEISEPSEPPQLDSGSSEAGLSQFDPKTMRNTKPGLKRLILTISVFSSFLLGLPFLWKT 60

Query: 61  VEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTKLS 120
           VEIYRAPLPFKDIDALS HIESSPLQFPCTFRV+FFGFDS+ASRAEQLKSSILDEMTKLS
Sbjct: 61  VEIYRAPLPFKDIDALSYHIESSPLQFPCTFRVLFFGFDSIASRAEQLKSSILDEMTKLS 120

Query: 121 SKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQSADD 180
           SKSSL GSCSNNYAVSVVIESGS+CSQTRTDASSC WRCGALSASDFAASLENG QSADD
Sbjct: 121 SKSSLYGSCSNNYAVSVVIESGSECSQTRTDASSCPWRCGALSASDFAASLENGFQSADD 180

Query: 181 FLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGKVSEAEAIAKVAETFV 240
           FLEVALGGCSKPASG RVYSVVVMNKGENV ATIGKYRHGWIVG+VSEA+A AKVAETFV
Sbjct: 181 FLEVALGGCSKPASGVRVYSVVVMNKGENVVATIGKYRHGWIVGRVSEADATAKVAETFV 240

Query: 241 KLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEE 300
           KLFGNGGTE GLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEE
Sbjct: 241 KLFGNGGTEGGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEE 300

Query: 301 LAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRS 360
           LAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRS
Sbjct: 301 LAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRS 360

Query: 361 KILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRH 420
           KILHFVVY+PSARECPLLL+LPDGQISETNGFISPTWGGVIVWNPKGCLRDHESK L RH
Sbjct: 361 KILHFVVYVPSARECPLLLELPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKQLRRH 420

Query: 421 MILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSC 480
           MI  PELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSC
Sbjct: 421 MISNPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSC 480

Query: 481 FNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSR 540
           FNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSR
Sbjct: 481 FNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSR 540

Query: 541 QARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKKYI 600
           QARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAA+REWKRYKQEHKKY+
Sbjct: 541 QARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAALREWKRYKQEHKKYL 600

Query: 601 AFLAKPKQS 610
           AFLAKPK+S
Sbjct: 601 AFLAKPKRS 609

BLAST of CSPI06G02220 vs. ExPASy TrEMBL
Match: A0A6J1KUG2 (GPI transamidase component PIG-S isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497728 PE=3 SV=1)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 539/613 (87.93%), Postives = 574/613 (93.64%), Query Frame = 0

Query: 1   MAEISEPSKPPQLDSGSSEAGLSQ--FDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLW 60
           MAEISEPS PP   SG S+A L Q  FDP TMR TKPGFKRLILTISVFSSFLLGLPFLW
Sbjct: 1   MAEISEPSNPPLAHSGLSDARLEQPAFDPNTMRKTKPGFKRLILTISVFSSFLLGLPFLW 60

Query: 61  KSVEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASR--AEQLKSSILDEM 120
           KSVEIYRAPLPF DIDALSS +ES+PLQFPC+FRVI+ GFDS+A R  AE+LKSSILDE+
Sbjct: 61  KSVEIYRAPLPFMDIDALSSQLESTPLQFPCSFRVIYVGFDSIAPRVSAEKLKSSILDEI 120

Query: 121 TKLSSKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQ 180
           T+LSSKSSLCGSC+NNYAVSVVIESGSDCSQTRTDASSC+WRCGALSASDFA SLENG  
Sbjct: 121 TELSSKSSLCGSCNNNYAVSVVIESGSDCSQTRTDASSCTWRCGALSASDFAVSLENGDD 180

Query: 181 SADDFLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGKVSEAEAIAKVA 240
           SADDFLEVALGGCS+ ASGGR YSVVVMN+ ENV+ TIGKYRH WIVG++SE EAI K+A
Sbjct: 181 SADDFLEVALGGCSRSASGGRGYSVVVMNRDENVEVTIGKYRHAWIVGRISEMEAIPKIA 240

Query: 241 ETFVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKP 300
           E FVKLFGNGG E+GLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQ VDEV+LKP
Sbjct: 241 EIFVKLFGNGGREEGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQTVDEVLLKP 300

Query: 301 LIEELAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAA 360
           LI+ELAP+ANVSVESQVLYHTPTSSFSYWD++QESYIFNTKDLPFFVNSNEWHLDTSIAA
Sbjct: 301 LIKELAPVANVSVESQVLYHTPTSSFSYWDDEQESYIFNTKDLPFFVNSNEWHLDTSIAA 360

Query: 361 GGRSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKL 420
           GGRSKILHFV+YIPSARECPLLLQLPDG ISETNGFISP WGGVIVWNPKGCL D +SK 
Sbjct: 361 GGRSKILHFVIYIPSARECPLLLQLPDGHISETNGFISPMWGGVIVWNPKGCL-DRKSKQ 420

Query: 421 LHRHMILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSR 480
            HR MI YPELEKIVEVFLGQFRQLFGLKS+PQHVGLSGTFNILTS+KGFT+WE+DFLSR
Sbjct: 421 HHRQMISYPELEKIVEVFLGQFRQLFGLKSDPQHVGLSGTFNILTSEKGFTKWEIDFLSR 480

Query: 481 QHSCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAA 540
           QHSCFNLHSCASSLGSLSRLVQSLPRMII+DEIGKQVKYSL+AANLAQ+NAS+GVFDAAA
Sbjct: 481 QHSCFNLHSCASSLGSLSRLVQSLPRMIILDEIGKQVKYSLKAANLAQRNASLGVFDAAA 540

Query: 541 ISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEH 600
           ISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAA+REWKRYKQEH
Sbjct: 541 ISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAALREWKRYKQEH 600

Query: 601 KKYIAFLAKPKQS 610
           KKY+AFLAKP QS
Sbjct: 601 KKYLAFLAKPNQS 612

BLAST of CSPI06G02220 vs. ExPASy TrEMBL
Match: A0A6J1H701 (GPI transamidase component PIG-S OS=Cucurbita moschata OX=3662 GN=LOC111461015 PE=3 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 1.1e-309
Identity = 539/611 (88.22%), Postives = 568/611 (92.96%), Query Frame = 0

Query: 1   MAEISEPSKPPQLDSGSSEAGLSQFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKS 60
           MAEISEPS PP   S         FDP TMR TKPGFKRLILTISVFSSFLLGLPFLWKS
Sbjct: 1   MAEISEPSNPPLEQSA--------FDPSTMRKTKPGFKRLILTISVFSSFLLGLPFLWKS 60

Query: 61  VEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASR--AEQLKSSILDEMTK 120
           VEIYRAPLPF DIDALSS +ES+PLQFPC+FRVI+ GFDS+A R  AEQLKSSILDE+T+
Sbjct: 61  VEIYRAPLPFMDIDALSSQLESTPLQFPCSFRVIYVGFDSIAPRVSAEQLKSSILDEITE 120

Query: 121 LSSKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQSA 180
           LSSKSSLCGSCSNNY VSVVIESGSDCSQTRTDASSCSWRCGALSASDFA SLENG  SA
Sbjct: 121 LSSKSSLCGSCSNNYDVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAVSLENGNDSA 180

Query: 181 DDFLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGKVSEAEAIAKVAET 240
           DDFLEVALGGCS+ ASGGR YSVVVMN+ ENV+ TIGKYRH WIVG++SE EAI KVAE 
Sbjct: 181 DDFLEVALGGCSRSASGGRGYSVVVMNRDENVEVTIGKYRHAWIVGRISEMEAIPKVAEI 240

Query: 241 FVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLI 300
           FVKLFGNGG E+GLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQ VDEV+LKPLI
Sbjct: 241 FVKLFGNGGREEGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQTVDEVLLKPLI 300

Query: 301 EELAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGG 360
           +ELAP+ANVSVESQVLYHTPTSSFSYWD+ QESYIFNTKDLPFFVNSNEWHLDTSIAAGG
Sbjct: 301 KELAPVANVSVESQVLYHTPTSSFSYWDDDQESYIFNTKDLPFFVNSNEWHLDTSIAAGG 360

Query: 361 RSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLH 420
           RSKILHFV+YIPSARECPLLLQLPDGQISETNGFISP WGGVIVWNPKGCL D +SK  H
Sbjct: 361 RSKILHFVIYIPSARECPLLLQLPDGQISETNGFISPMWGGVIVWNPKGCL-DRKSKQHH 420

Query: 421 RHMILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQH 480
           R MI YPELEKIVEVFLGQFRQLFGLKS+PQHVGLSGTFNILTS+KGFT+WE+DFLSRQH
Sbjct: 421 RQMISYPELEKIVEVFLGQFRQLFGLKSDPQHVGLSGTFNILTSEKGFTKWEIDFLSRQH 480

Query: 481 SCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAIS 540
           SCFNLHSCASSLGSLSRLVQSLPRMII+DEIGKQVKYSL+AANLAQ+NAS+GVFDAAAIS
Sbjct: 481 SCFNLHSCASSLGSLSRLVQSLPRMIILDEIGKQVKYSLKAANLAQRNASLGVFDAAAIS 540

Query: 541 SRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKK 600
           SRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAA+REWKRYKQEHKK
Sbjct: 541 SRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAALREWKRYKQEHKK 600

Query: 601 YIAFLAKPKQS 610
           Y+AFLAKPKQS
Sbjct: 601 YLAFLAKPKQS 602

BLAST of CSPI06G02220 vs. ExPASy TrEMBL
Match: A0A6J1KS04 (GPI transamidase component PIG-S isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111497728 PE=3 SV=1)

HSP 1 Score: 1064.7 bits (2752), Expect = 1.5e-307
Identity = 535/613 (87.28%), Postives = 569/613 (92.82%), Query Frame = 0

Query: 1   MAEISEPSKPPQLDSGSSEAGLSQ--FDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLW 60
           MAEISEPS PP          L Q  FDP TMR TKPGFKRLILTISVFSSFLLGLPFLW
Sbjct: 1   MAEISEPSNPP----------LEQPAFDPNTMRKTKPGFKRLILTISVFSSFLLGLPFLW 60

Query: 61  KSVEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASR--AEQLKSSILDEM 120
           KSVEIYRAPLPF DIDALSS +ES+PLQFPC+FRVI+ GFDS+A R  AE+LKSSILDE+
Sbjct: 61  KSVEIYRAPLPFMDIDALSSQLESTPLQFPCSFRVIYVGFDSIAPRVSAEKLKSSILDEI 120

Query: 121 TKLSSKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQ 180
           T+LSSKSSLCGSC+NNYAVSVVIESGSDCSQTRTDASSC+WRCGALSASDFA SLENG  
Sbjct: 121 TELSSKSSLCGSCNNNYAVSVVIESGSDCSQTRTDASSCTWRCGALSASDFAVSLENGDD 180

Query: 181 SADDFLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGKVSEAEAIAKVA 240
           SADDFLEVALGGCS+ ASGGR YSVVVMN+ ENV+ TIGKYRH WIVG++SE EAI K+A
Sbjct: 181 SADDFLEVALGGCSRSASGGRGYSVVVMNRDENVEVTIGKYRHAWIVGRISEMEAIPKIA 240

Query: 241 ETFVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKP 300
           E FVKLFGNGG E+GLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQ VDEV+LKP
Sbjct: 241 EIFVKLFGNGGREEGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQTVDEVLLKP 300

Query: 301 LIEELAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAA 360
           LI+ELAP+ANVSVESQVLYHTPTSSFSYWD++QESYIFNTKDLPFFVNSNEWHLDTSIAA
Sbjct: 301 LIKELAPVANVSVESQVLYHTPTSSFSYWDDEQESYIFNTKDLPFFVNSNEWHLDTSIAA 360

Query: 361 GGRSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKL 420
           GGRSKILHFV+YIPSARECPLLLQLPDG ISETNGFISP WGGVIVWNPKGCL D +SK 
Sbjct: 361 GGRSKILHFVIYIPSARECPLLLQLPDGHISETNGFISPMWGGVIVWNPKGCL-DRKSKQ 420

Query: 421 LHRHMILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSR 480
            HR MI YPELEKIVEVFLGQFRQLFGLKS+PQHVGLSGTFNILTS+KGFT+WE+DFLSR
Sbjct: 421 HHRQMISYPELEKIVEVFLGQFRQLFGLKSDPQHVGLSGTFNILTSEKGFTKWEIDFLSR 480

Query: 481 QHSCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAA 540
           QHSCFNLHSCASSLGSLSRLVQSLPRMII+DEIGKQVKYSL+AANLAQ+NAS+GVFDAAA
Sbjct: 481 QHSCFNLHSCASSLGSLSRLVQSLPRMIILDEIGKQVKYSLKAANLAQRNASLGVFDAAA 540

Query: 541 ISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEH 600
           ISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAA+REWKRYKQEH
Sbjct: 541 ISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAALREWKRYKQEH 600

Query: 601 KKYIAFLAKPKQS 610
           KKY+AFLAKP QS
Sbjct: 601 KKYLAFLAKPNQS 602

BLAST of CSPI06G02220 vs. NCBI nr
Match: XP_004138391.1 (GPI transamidase component PIG-S [Cucumis sativus] >KGN45842.1 hypothetical protein Csa_004823 [Cucumis sativus])

HSP 1 Score: 1210.3 bits (3130), Expect = 0.0e+00
Identity = 606/609 (99.51%), Postives = 607/609 (99.67%), Query Frame = 0

Query: 1   MAEISEPSKPPQLDSGSSEAGLSQFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKS 60
           MAEISEPSKPPQLDSGSSEAGLSQFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKS
Sbjct: 1   MAEISEPSKPPQLDSGSSEAGLSQFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKS 60

Query: 61  VEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTKLS 120
           VEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTKLS
Sbjct: 61  VEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTKLS 120

Query: 121 SKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQSADD 180
           SKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQSADD
Sbjct: 121 SKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQSADD 180

Query: 181 FLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGKVSEAEAIAKVAETFV 240
           FLEVALGGC KPASGGRVYSVVVMNKGENVKATIGKYRHGWIVG+VSEAEAIAKVAETFV
Sbjct: 181 FLEVALGGCYKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGRVSEAEAIAKVAETFV 240

Query: 241 KLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEE 300
           KLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEE
Sbjct: 241 KLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEE 300

Query: 301 LAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRS 360
           L PIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRS
Sbjct: 301 LTPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRS 360

Query: 361 KILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRH 420
           KILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRH
Sbjct: 361 KILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRH 420

Query: 421 MILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSC 480
           MILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSC
Sbjct: 421 MILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSC 480

Query: 481 FNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSR 540
           FNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSR
Sbjct: 481 FNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSR 540

Query: 541 QARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKKYI 600
           QARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKKYI
Sbjct: 541 QARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKKYI 600

Query: 601 AFLAKPKQS 610
           AFLAKPKQS
Sbjct: 601 AFLAKPKQS 609

BLAST of CSPI06G02220 vs. NCBI nr
Match: XP_008456783.1 (PREDICTED: GPI transamidase component PIG-S [Cucumis melo])

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 583/609 (95.73%), Postives = 595/609 (97.70%), Query Frame = 0

Query: 1   MAEISEPSKPPQLDSGSSEAGLSQFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKS 60
           M EISEPS+PPQLDSGSSEAGLSQFDPKTMRNTKPG KRLILTISVFSSFLLGLPFLWK+
Sbjct: 1   MGEISEPSEPPQLDSGSSEAGLSQFDPKTMRNTKPGLKRLILTISVFSSFLLGLPFLWKT 60

Query: 61  VEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTKLS 120
           VEIYRAPLPFKDIDALS HIESSPLQFPCTFRV+FFGFDS+ASRAEQLKSSILDEMTKLS
Sbjct: 61  VEIYRAPLPFKDIDALSYHIESSPLQFPCTFRVLFFGFDSIASRAEQLKSSILDEMTKLS 120

Query: 121 SKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQSADD 180
           SKSSL GSCSNNYAVSVVIESGS+CSQTRTDASSC WRCGALSASDFAASLENG QSADD
Sbjct: 121 SKSSLYGSCSNNYAVSVVIESGSECSQTRTDASSCPWRCGALSASDFAASLENGFQSADD 180

Query: 181 FLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGKVSEAEAIAKVAETFV 240
           FLEVALGGCSKPASG RVYSVVVMNKGENV ATIGKYRHGWIVG+VSEA+A AKVAETFV
Sbjct: 181 FLEVALGGCSKPASGVRVYSVVVMNKGENVVATIGKYRHGWIVGRVSEADATAKVAETFV 240

Query: 241 KLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEE 300
           KLFGNGGTE GLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEE
Sbjct: 241 KLFGNGGTEGGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEE 300

Query: 301 LAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRS 360
           LAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRS
Sbjct: 301 LAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRS 360

Query: 361 KILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRH 420
           KILHFVVY+PSARECPLLL+LPDGQISETNGFISPTWGGVIVWNPKGCLRDHESK L RH
Sbjct: 361 KILHFVVYVPSARECPLLLELPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKQLRRH 420

Query: 421 MILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSC 480
           MI  PELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSC
Sbjct: 421 MISNPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSC 480

Query: 481 FNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSR 540
           FNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSR
Sbjct: 481 FNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSR 540

Query: 541 QARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKKYI 600
           QARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAA+REWKRYKQEHKKY+
Sbjct: 541 QARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAALREWKRYKQEHKKYL 600

Query: 601 AFLAKPKQS 610
           AFLAKPK+S
Sbjct: 601 AFLAKPKRS 609

BLAST of CSPI06G02220 vs. NCBI nr
Match: XP_038898358.1 (GPI transamidase component PIG-S [Benincasa hispida])

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 569/611 (93.13%), Postives = 590/611 (96.56%), Query Frame = 0

Query: 1   MAEISEPSKPPQLDSGSSEAGLSQ--FDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLW 60
           MAEISEPSKPPQLDSGSSEAGLSQ  FDPKTMR TKPGFKRLILTISVFSSFLLGLPFLW
Sbjct: 1   MAEISEPSKPPQLDSGSSEAGLSQFAFDPKTMRKTKPGFKRLILTISVFSSFLLGLPFLW 60

Query: 61  KSVEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTK 120
           KSVEIYRAPLPF+DIDALSSHIESSPLQFPCTFRVIF GFDSMAS AEQLKSS+  EMTK
Sbjct: 61  KSVEIYRAPLPFRDIDALSSHIESSPLQFPCTFRVIFIGFDSMASTAEQLKSSLFHEMTK 120

Query: 121 LSSKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQSA 180
           LSSKSSLCGSC+NN+A+SVVIESGSDCSQTRTDASSCSWRCGALSAS+FAASLENGL S 
Sbjct: 121 LSSKSSLCGSCNNNFAISVVIESGSDCSQTRTDASSCSWRCGALSASNFAASLENGLHST 180

Query: 181 DDFLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGKVSEAEAIAKVAET 240
           DDFLEVALGGCS+ ASGG+VYSVVVMN  ENV+ATIGKYRHGWIVG+VSEAEAI KVAET
Sbjct: 181 DDFLEVALGGCSRSASGGKVYSVVVMNGDENVEATIGKYRHGWIVGRVSEAEAILKVAET 240

Query: 241 FVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLI 300
           FVKLFGNGG EDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQ VDEV+LKPLI
Sbjct: 241 FVKLFGNGGREDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQTVDEVLLKPLI 300

Query: 301 EELAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGG 360
           EELAP+AN+SVESQVLYHTPTSSFSYWD++QESYIFNTKDLPFFVNSNEWHLDTSIAAGG
Sbjct: 301 EELAPVANISVESQVLYHTPTSSFSYWDDEQESYIFNTKDLPFFVNSNEWHLDTSIAAGG 360

Query: 361 RSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLH 420
           RSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCL+D ESK  H
Sbjct: 361 RSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLKDRESKQHH 420

Query: 421 RHMILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQH 480
           RHMI YPELEKIVEVFLGQFRQLFGLKSNPQH G+SGTFNILTSQKGFTEWEMDFLSRQH
Sbjct: 421 RHMISYPELEKIVEVFLGQFRQLFGLKSNPQHGGVSGTFNILTSQKGFTEWEMDFLSRQH 480

Query: 481 SCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAIS 540
           SCFNLHSCASSLGSLS+LVQSLPRMIIIDEIGKQVKYSLEAANLAQKNAS GVFDAAAIS
Sbjct: 481 SCFNLHSCASSLGSLSKLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASAGVFDAAAIS 540

Query: 541 SRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKK 600
           SRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAA+REWKRYKQEHKK
Sbjct: 541 SRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAALREWKRYKQEHKK 600

Query: 601 YIAFLAKPKQS 610
           Y+AFLA+PKQS
Sbjct: 601 YLAFLAEPKQS 611

BLAST of CSPI06G02220 vs. NCBI nr
Match: XP_023004395.1 (GPI transamidase component PIG-S isoform X1 [Cucurbita maxima])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 539/613 (87.93%), Postives = 574/613 (93.64%), Query Frame = 0

Query: 1   MAEISEPSKPPQLDSGSSEAGLSQ--FDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLW 60
           MAEISEPS PP   SG S+A L Q  FDP TMR TKPGFKRLILTISVFSSFLLGLPFLW
Sbjct: 1   MAEISEPSNPPLAHSGLSDARLEQPAFDPNTMRKTKPGFKRLILTISVFSSFLLGLPFLW 60

Query: 61  KSVEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASR--AEQLKSSILDEM 120
           KSVEIYRAPLPF DIDALSS +ES+PLQFPC+FRVI+ GFDS+A R  AE+LKSSILDE+
Sbjct: 61  KSVEIYRAPLPFMDIDALSSQLESTPLQFPCSFRVIYVGFDSIAPRVSAEKLKSSILDEI 120

Query: 121 TKLSSKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQ 180
           T+LSSKSSLCGSC+NNYAVSVVIESGSDCSQTRTDASSC+WRCGALSASDFA SLENG  
Sbjct: 121 TELSSKSSLCGSCNNNYAVSVVIESGSDCSQTRTDASSCTWRCGALSASDFAVSLENGDD 180

Query: 181 SADDFLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGKVSEAEAIAKVA 240
           SADDFLEVALGGCS+ ASGGR YSVVVMN+ ENV+ TIGKYRH WIVG++SE EAI K+A
Sbjct: 181 SADDFLEVALGGCSRSASGGRGYSVVVMNRDENVEVTIGKYRHAWIVGRISEMEAIPKIA 240

Query: 241 ETFVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKP 300
           E FVKLFGNGG E+GLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQ VDEV+LKP
Sbjct: 241 EIFVKLFGNGGREEGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQTVDEVLLKP 300

Query: 301 LIEELAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAA 360
           LI+ELAP+ANVSVESQVLYHTPTSSFSYWD++QESYIFNTKDLPFFVNSNEWHLDTSIAA
Sbjct: 301 LIKELAPVANVSVESQVLYHTPTSSFSYWDDEQESYIFNTKDLPFFVNSNEWHLDTSIAA 360

Query: 361 GGRSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKL 420
           GGRSKILHFV+YIPSARECPLLLQLPDG ISETNGFISP WGGVIVWNPKGCL D +SK 
Sbjct: 361 GGRSKILHFVIYIPSARECPLLLQLPDGHISETNGFISPMWGGVIVWNPKGCL-DRKSKQ 420

Query: 421 LHRHMILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSR 480
            HR MI YPELEKIVEVFLGQFRQLFGLKS+PQHVGLSGTFNILTS+KGFT+WE+DFLSR
Sbjct: 421 HHRQMISYPELEKIVEVFLGQFRQLFGLKSDPQHVGLSGTFNILTSEKGFTKWEIDFLSR 480

Query: 481 QHSCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAA 540
           QHSCFNLHSCASSLGSLSRLVQSLPRMII+DEIGKQVKYSL+AANLAQ+NAS+GVFDAAA
Sbjct: 481 QHSCFNLHSCASSLGSLSRLVQSLPRMIILDEIGKQVKYSLKAANLAQRNASLGVFDAAA 540

Query: 541 ISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEH 600
           ISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAA+REWKRYKQEH
Sbjct: 541 ISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAALREWKRYKQEH 600

Query: 601 KKYIAFLAKPKQS 610
           KKY+AFLAKP QS
Sbjct: 601 KKYLAFLAKPNQS 612

BLAST of CSPI06G02220 vs. NCBI nr
Match: XP_023513935.1 (GPI transamidase component PIG-S isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 540/613 (88.09%), Postives = 573/613 (93.47%), Query Frame = 0

Query: 1   MAEISEPSKPPQLDSGSSEAGLSQ--FDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLW 60
           MAEISEPS PP + SG  +A L Q  FDP TMR TKPGFKRLILTISVFSSFLLGLPFLW
Sbjct: 1   MAEISEPSNPPLVHSGLPDARLEQSAFDPSTMRKTKPGFKRLILTISVFSSFLLGLPFLW 60

Query: 61  KSVEIYRAPLPFKDIDALSSHIESSPLQFPCTFRVIFFGFDSMASR--AEQLKSSILDEM 120
           KSVEIYRAPLPF DID LSS +ES+PLQFPC+FRVI+ GFDS+A R  AEQLKSSILDE+
Sbjct: 61  KSVEIYRAPLPFMDIDVLSSQLESTPLQFPCSFRVIYVGFDSIAPRVSAEQLKSSILDEI 120

Query: 121 TKLSSKSSLCGSCSNNYAVSVVIESGSDCSQTRTDASSCSWRCGALSASDFAASLENGLQ 180
           T+LSSKSSLCGSCSNNY VSVVIESGSDCSQTRTDASSC+WRCGALSASDFA SLENG  
Sbjct: 121 TELSSKSSLCGSCSNNYDVSVVIESGSDCSQTRTDASSCTWRCGALSASDFAVSLENGDD 180

Query: 181 SADDFLEVALGGCSKPASGGRVYSVVVMNKGENVKATIGKYRHGWIVGKVSEAEAIAKVA 240
           SADDFLEVALGGCS+ ASGGR YSVVVMN+ ENV+ TIGKYRH WIVG++SE EAI KVA
Sbjct: 181 SADDFLEVALGGCSRSASGGRGYSVVVMNRDENVEVTIGKYRHAWIVGRISEMEAIPKVA 240

Query: 241 ETFVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKP 300
           E FVKLFGNGG E+GLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQ VDEV+LKP
Sbjct: 241 EIFVKLFGNGGREEGLIPGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQTVDEVLLKP 300

Query: 301 LIEELAPIANVSVESQVLYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAA 360
           LI+ELAP+ANVSVESQVLYHTPTSSFSYWD+ QESYIFNTKDLPFFVNSNEWHLDTSIAA
Sbjct: 301 LIKELAPVANVSVESQVLYHTPTSSFSYWDDDQESYIFNTKDLPFFVNSNEWHLDTSIAA 360

Query: 361 GGRSKILHFVVYIPSARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKL 420
           GGRSKILHFV+YIPSARECPLLLQLP+GQISETNGFISP WGGVIVWNPKGCL D +SK 
Sbjct: 361 GGRSKILHFVIYIPSARECPLLLQLPNGQISETNGFISPMWGGVIVWNPKGCL-DRKSKQ 420

Query: 421 LHRHMILYPELEKIVEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSR 480
            HR MI YPELEKIVEVFLGQFRQLFGLKS+PQHVGLSGTFNILTS+KGFT+WE+DFLSR
Sbjct: 421 HHRQMISYPELEKIVEVFLGQFRQLFGLKSDPQHVGLSGTFNILTSEKGFTKWEIDFLSR 480

Query: 481 QHSCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAA 540
           QHSCFNLHSCASSLGSLSRLVQSLPRMII+DEIGKQVKYSL+AANLAQ+NAS+GVFDAAA
Sbjct: 481 QHSCFNLHSCASSLGSLSRLVQSLPRMIILDEIGKQVKYSLKAANLAQRNASLGVFDAAA 540

Query: 541 ISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEH 600
           ISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAA+REWKRYKQEH
Sbjct: 541 ISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYSPFFLPVALHVILAALREWKRYKQEH 600

Query: 601 KKYIAFLAKPKQS 610
           KKY+AFLAKPKQS
Sbjct: 601 KKYLAFLAKPKQS 612

BLAST of CSPI06G02220 vs. TAIR 10
Match: AT3G07180.1 (GPI transamidase component PIG-S-related )

HSP 1 Score: 731.5 bits (1887), Expect = 5.6e-211
Identity = 369/594 (62.12%), Postives = 464/594 (78.11%), Query Frame = 0

Query: 24  QFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKSVEIYRAPLPFKDIDALSSHIESS 83
           +FDPKTMR+TKPG KRL +T SV  SFLLG+PFLWKSVEIYR+PLPF DID+LS  +ES+
Sbjct: 15  EFDPKTMRSTKPGLKRLFITSSVLFSFLLGVPFLWKSVEIYRSPLPFHDIDSLSDQLEST 74

Query: 84  PLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTKLSSKSSLCGSCSNNYAVSVVIESGS 143
           PL+FPC F  +F GF S  +  + L+S I D + +L+ +SS CGSC  ++++SV +++  
Sbjct: 75  PLRFPCNFHAVFVGFRS--TDPDNLRSQIQDGINQLTHQSSQCGSC--DFSLSVTVQNRE 134

Query: 144 D-CSQTRTDAS-SCSWRCGALSASDFAASLENGLQSADDFLEVALGGCSKPASGGRVYSV 203
           D CS T   +S +CS+RCG +  + F+  L++   + D+ L     GCS+  + G++YSV
Sbjct: 135 DQCSDTLAHSSTTCSYRCGVIKRNGFSVGLDD---TVDESLNDVFSGCSE--NSGKMYSV 194

Query: 204 VVMNK-----GENVKATIGKYRHGWIVGKVSE---AEAIAKVAETFVKLFGNGGTEDGLI 263
           VV+NK     G+ VKA +GK RH WIVG   E    + +A+V+E FV++F NGG E+  I
Sbjct: 195 VVVNKENANGGDEVKAVVGKRRHAWIVGNGLEERYGDIVARVSEIFVQVFMNGGREEDSI 254

Query: 264 PGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEELAPIANVSVESQV 323
            GEFMPVG+DGKI LSF+LLN++P DW+YDWDFQ++DE +L P+ + LAPIAN++VESQV
Sbjct: 255 QGEFMPVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQV 314

Query: 324 LYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRSKILHFVVYIPSAR 383
           LYHTP SSFS WD K +SYIF T DLPFFVNSNEWHLDTS  A GRSKIL FVVYIPS +
Sbjct: 315 LYHTPKSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGK 374

Query: 384 ECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRHMILYPELEKIVEV 443
           ECPLLLQLP+ +IS+TNGFISP WGGVIVWNP  C +D ES    R+ I   +LE+IVE+
Sbjct: 375 ECPLLLQLPNVEISKTNGFISPMWGGVIVWNPGNCDKDSESP--SRNTISLQDLEQIVEI 434

Query: 444 FLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSCFNLHSCASSLGSL 503
           FLGQFRQLFG KS  ++    GT+ ILTS++GFTEWE+D LSR+H+CFNLHSC+++LGSL
Sbjct: 435 FLGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSL 494

Query: 504 SRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSRQARSLAEDAFFHP 563
           SRLV+SLPRMII DEIG+QVKYSL+AA LAQ NAS+G + ++A SSR+ARSLAE+AFFHP
Sbjct: 495 SRLVRSLPRMIIKDEIGEQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHP 554

Query: 564 SIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKKYIAFLAKPK 608
           SIMSVSYFS+EHCFAVYSPFFLPV  HV+LAAVREWKRYKQE  KY+ +L + K
Sbjct: 555 SIMSVSYFSYEHCFAVYSPFFLPVVGHVVLAAVREWKRYKQEKAKYLTWLTRKK 597

BLAST of CSPI06G02220 vs. TAIR 10
Match: AT3G07180.3 (GPI transamidase component PIG-S-related )

HSP 1 Score: 707.2 bits (1824), Expect = 1.1e-203
Identity = 362/594 (60.94%), Postives = 456/594 (76.77%), Query Frame = 0

Query: 24  QFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKSVEIYRAPLPFKDIDALSSHIESS 83
           +FDPKTMR+TKPG KRL +T SV  SFLL        VEIYR+PLPF DID+LS  +ES+
Sbjct: 15  EFDPKTMRSTKPGLKRLFITSSVLFSFLL--------VEIYRSPLPFHDIDSLSDQLEST 74

Query: 84  PLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTKLSSKSSLCGSCSNNYAVSVVIESGS 143
           PL+FPC F  +F GF S  +  + L+S I D + +L+ +SS CGSC  ++++SV +++  
Sbjct: 75  PLRFPCNFHAVFVGFRS--TDPDNLRSQIQDGINQLTHQSSQCGSC--DFSLSVTVQNRE 134

Query: 144 D-CSQTRTDAS-SCSWRCGALSASDFAASLENGLQSADDFLEVALGGCSKPASGGRVYSV 203
           D CS T   +S +CS+RCG +  + F+  L++   + D+ L     GCS+  + G++YSV
Sbjct: 135 DQCSDTLAHSSTTCSYRCGVIKRNGFSVGLDD---TVDESLNDVFSGCSE--NSGKMYSV 194

Query: 204 VVMNK-----GENVKATIGKYRHGWIVGKVSE---AEAIAKVAETFVKLFGNGGTEDGLI 263
           VV+NK     G+ VKA +GK RH WIVG   E    + +A+V+E FV++F NGG E+  I
Sbjct: 195 VVVNKENANGGDEVKAVVGKRRHAWIVGNGLEERYGDIVARVSEIFVQVFMNGGREEDSI 254

Query: 264 PGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEELAPIANVSVESQV 323
            GEFMPVG+DGKI LSF+LLN++P DW+YDWDFQ++DE +L P+ + LAPIAN++VESQV
Sbjct: 255 QGEFMPVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQV 314

Query: 324 LYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRSKILHFVVYIPSAR 383
           LYHTP SSFS WD K +SYIF T DLPFFVNSNEWHLDTS  A GRSKIL FVVYIPS +
Sbjct: 315 LYHTPKSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGK 374

Query: 384 ECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRHMILYPELEKIVEV 443
           ECPLLLQLP+ +IS+TNGFISP WGGVIVWNP  C +D ES    R+ I   +LE+IVE+
Sbjct: 375 ECPLLLQLPNVEISKTNGFISPMWGGVIVWNPGNCDKDSESP--SRNTISLQDLEQIVEI 434

Query: 444 FLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSCFNLHSCASSLGSL 503
           FLGQFRQLFG KS  ++    GT+ ILTS++GFTEWE+D LSR+H+CFNLHSC+++LGSL
Sbjct: 435 FLGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSL 494

Query: 504 SRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSRQARSLAEDAFFHP 563
           SRLV+SLPRMII DEIG+QVKYSL+AA LAQ NAS+G + ++A SSR+ARSLAE+AFFHP
Sbjct: 495 SRLVRSLPRMIIKDEIGEQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHP 554

Query: 564 SIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKKYIAFLAKPK 608
           SIMSVSYFS+EHCFAVYSPFFLPV  HV+LAAVREWKRYKQE  KY+ +L + K
Sbjct: 555 SIMSVSYFSYEHCFAVYSPFFLPVVGHVVLAAVREWKRYKQEKAKYLTWLTRKK 589

BLAST of CSPI06G02220 vs. TAIR 10
Match: AT3G07180.2 (GPI transamidase component PIG-S-related )

HSP 1 Score: 464.2 bits (1193), Expect = 1.7e-130
Identity = 269/594 (45.29%), Postives = 342/594 (57.58%), Query Frame = 0

Query: 24  QFDPKTMRNTKPGFKRLILTISVFSSFLLGLPFLWKSVEIYRAPLPFKDIDALSSHIESS 83
           +FDPKTMR+TKPG KRL +T SV  SFLLG+PFLWKSVEIYR+PLPF DID+LS  +ES+
Sbjct: 15  EFDPKTMRSTKPGLKRLFITSSVLFSFLLGVPFLWKSVEIYRSPLPFHDIDSLSDQLEST 74

Query: 84  PLQFPCTFRVIFFGFDSMASRAEQLKSSILDEMTKLSSKSSLCGSCSNNYAVSVVIESGS 143
           PL+FPC F  +F GF S  +  + L+S I D + +L+ +SS CGSC  ++++SV +++  
Sbjct: 75  PLRFPCNFHAVFVGFRS--TDPDNLRSQIQDGINQLTHQSSQCGSC--DFSLSVTVQNRE 134

Query: 144 D-CSQTRTDAS-SCSWRCGALSASDFAASLENGLQSADDFLEVALGGCSKPASGGRVYSV 203
           D CS T   +S +CS+RCG +  + F+  L++   + D+ L     GCS+  + G++YSV
Sbjct: 135 DQCSDTLAHSSTTCSYRCGVIKRNGFSVGLDD---TVDESLNDVFSGCSE--NSGKMYSV 194

Query: 204 VVMNK-----GENVKATIGKYRHGWIVGKVSE---AEAIAKVAETFVKLFGNGGTEDGLI 263
           VV+NK     G+ VKA +GK RH WIVG   E    + +A+V+E FV++F NGG E+  I
Sbjct: 195 VVVNKENANGGDEVKAVVGKRRHAWIVGNGLEERYGDIVARVSEIFVQVFMNGGREEDSI 254

Query: 264 PGEFMPVGADGKIFLSFNLLNADPDDWIYDWDFQKVDEVILKPLIEELAPIANVSVESQV 323
            GEFMPVG+DGKI LSF+LLN++P DW+YDWDFQ++DE +L P+ + LAPIAN++VESQV
Sbjct: 255 QGEFMPVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQV 314

Query: 324 LYHTPTSSFSYWDNKQESYIFNTKDLPFFVNSNEWHLDTSIAAGGRSKILHFVVYIPSAR 383
           LYHTP SSFS WD K +SYIF T DLPFFVNSNEWHLDTS  A GRSKIL F        
Sbjct: 315 LYHTPKSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQF-------- 374

Query: 384 ECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRHMILYPELEKIVEV 443
                                                                       
Sbjct: 375 ------------------------------------------------------------ 434

Query: 444 FLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSCFNLHSCASSLGSL 503
                                                                       
Sbjct: 435 ------------------------------------------------------------ 452

Query: 504 SRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSRQARSLAEDAFFHP 563
                              VKYSL+AA LAQ NAS+G + ++A SSR+ARSLAE+AFFHP
Sbjct: 495 -------------------VKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHP 452

Query: 564 SIMSVSYFSFEHCFAVYSPFFLPVALHVILAAVREWKRYKQEHKKYIAFLAKPK 608
           SIMSVSYFS+EHCFAVYSPFFLPV  HV+LAAVREWKRYKQE  KY+ +L + K
Sbjct: 555 SIMSVSYFSYEHCFAVYSPFFLPVVGHVVLAAVREWKRYKQEKAKYLTWLTRKK 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6PD261.2e-4327.26GPI transamidase component PIG-S OS=Mus musculus OX=10090 GN=Pigs PE=1 SV=3[more]
Q3SZL55.9e-4027.03GPI transamidase component PIG-S OS=Bos taurus OX=9913 GN=PIGS PE=2 SV=3[more]
Q96S521.7e-3924.96GPI transamidase component PIG-S OS=Homo sapiens OX=9606 GN=PIGS PE=1 SV=3[more]
Q5XI312.5e-3829.06GPI transamidase component PIG-S OS=Rattus norvegicus OX=10116 GN=Pigs PE=2 SV=3[more]
Q103511.3e-2629.66GPI transamidase component PIG-S homolog OS=Schizosaccharomyces pombe (strain 97... [more]
Match NameE-valueIdentityDescription
A0A0A0KBT30.0e+0099.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014620 PE=3 SV=1[more]
A0A1S3C4Q80.0e+0095.73GPI transamidase component PIG-S OS=Cucumis melo OX=3656 GN=LOC103496630 PE=3 SV... [more]
A0A6J1KUG20.0e+0087.93GPI transamidase component PIG-S isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1H7011.1e-30988.22GPI transamidase component PIG-S OS=Cucurbita moschata OX=3662 GN=LOC111461015 P... [more]
A0A6J1KS041.5e-30787.28GPI transamidase component PIG-S isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_004138391.10.0e+0099.51GPI transamidase component PIG-S [Cucumis sativus] >KGN45842.1 hypothetical prot... [more]
XP_008456783.10.0e+0095.73PREDICTED: GPI transamidase component PIG-S [Cucumis melo][more]
XP_038898358.10.0e+0093.13GPI transamidase component PIG-S [Benincasa hispida][more]
XP_023004395.10.0e+0087.93GPI transamidase component PIG-S isoform X1 [Cucurbita maxima][more]
XP_023513935.10.0e+0088.09GPI transamidase component PIG-S isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G07180.15.6e-21162.12GPI transamidase component PIG-S-related [more]
AT3G07180.31.1e-20360.94GPI transamidase component PIG-S-related [more]
AT3G07180.21.7e-13045.29GPI transamidase component PIG-S-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019540Phosphatidylinositol-glycan biosynthesis class S proteinPFAMPF10510PIG-Scoord: 47..594
e-value: 5.2E-129
score: 431.4
IPR019540Phosphatidylinositol-glycan biosynthesis class S proteinPANTHERPTHR21072GPI TRANSAMIDASE COMPONENT PIG-Scoord: 25..604
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..30

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G02220.1CSPI06G02220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016255 attachment of GPI anchor to protein
cellular_component GO:0042765 GPI-anchor transamidase complex