CSPI06G00210 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G00210
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	tRNA pseudouridine synthase
Location	Chr6: 189535 .. 192681 (+)
RNA-Seq Expression	CSPI06G00210
Synteny	CSPI06G00210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAACTATCTTCAATATTTGTATGTTTCTTAGTTCTTAGCTTGATTGTTAAACCCGGTTGATGAGAGATATCAAAATTGAATTTATAGAAAGGTGAGGGCTTCCAAGAGTTTATGCAAATATCATCAACTGAAAGCATGATTCTGTATCCTATAAAAAGAGAGAAATTTAGTCATAGGATATTTTGTTTTCATATATATAATATGTAAAATTTATGAGTTTTGAATTTTCACCCACATATTGTCACCAAAAGGGAAATCTCTGAATTGAAAACTCAACGGATGAGGTGCTGGGCGGTGGGTTCGCCTGTTCTTCCTCCAATTTCCCTTCCCCTTTCACCAATCATTCCATCTCCAAATGGCGCTTCATCTTCCTCCAGTCGTGGATTCCCGGTAACGACAATTCCTTTTCTGATCTCTCAGTTCCTCTTTTCATTTTGGTGGTTTAATTTATTTGATCGCGCCTTGAAAAGAATGCGGAGGATGATGCCTCTTATATCAAAGAGCCTTCTTTCCCATGCTTCAAATGGCGGTTGCTCATCGCTTATGACGGTACTCGATATGCAGGTCCAAGTTTCTTCTTCTTCTCTTTAATTGTCTATTCAGGCGAATGAATTGAACGATTTTGTGTTCGTATCCCAGCTTTATCTTTTTGGGTTAACAGTAATTGTAAATAGCATAAGGTTTTGAGGTTAGTTGTTGTTGCCTGTTGGATGTCTTCCATCACCAGCATCAGTTTCACTTCAGGTTCACCCTAAAGGGAGACTCGTTTAGAAGTACTTAGCCTGCTTTTATTACGAGTTAATTTGGAGAAGTTTGTGCTCACTGCTCAAAATTTGAGGCTTTCTTGTTACATGATATAATATTAAACTTATATTTATCCATAAGTTTAGGGTTATGGGTCAATCGGTGATTTGAGATGGTATTGGTTCAAAATTTGTTGTGTTTAAACTCTTGCAATATTATTTTCTCTCAAATTAATATTGACTTTCACCTGGTTCAAATTCATAAGTGAGGGAGTGTTAGATAATACCATATAAATTTATCTTCACCGAAGCTTAAGTTGTTGGGTCAGTCAGTAATTGAAGACTTGGTGCAAAAGTTTGATAAAATAGTTTTGAATATGTTGATATTAAACTAGGCTGGCAATATCAGAGGTCACCACCCACTATCCAGTGCCTCATTGAAGAAGCCTTGATTGGAGCAACAAAGCTTGAAAGGAAGGATCTTCATTTAGTTGGTGCAAGCAGAACAGATAAAGGAGTACATGCCTGGGGTCAGGTCTGATGTGATTTTATGGAGTTGGGTTAAAACACATAATTGGAATACAATGCTTGCTGTTGCTTACGACAAGTTGAGATTTCAGGTGGCTCACTTTGTTACGCCATTTAACTACGATAGCTTGGAAAGCATTCATGCTGCACTGAATGGCCTTCTTCCTGTGGATATAAGAGTTCGAGAGATGAGTCCTGCTGTACCCGATTTTCATGCTCGGTTTTCTACGTTAAGCAAGGTTTATCACTACAAGATATATAGTGGCACAATCATGGATCCATTTCAGCGTTGCTACTCTTACCACTGTGTTTATAAACTCAACTATGCAGCCATGAAAGAAGCTGCAAATTATTTCGTCGGAAAGCATGATTTTTCTGCTTTTACGAATGCAGCTCATAACGATCGAGTGGTAAATCCAGTGAAGGTCATTTTCCGTTTTGATGTGATAGAAATGGTAAAGTTTTAAGGTCCTTTAATTAGTGATTTGTTTGGTTGGAATTACCAATTTAGTCTATAGCCTTCGAAATTTATGTCTATTTGGTCATCTTTCAAAAGTGTTCAATAGGTCATTGCACTTCAATCTTGTGCCATATAGGACCTTGAACTTCAAAGTGTATTGGAAGGTTCTTAAACTTTCAATTTCATGTCCATTAGATCTCTTATTTTCAATTTTGTATTTATCCTTGACGTATTCGATAATTGCTAAAATTCACAGATTTATTGACATAAAATCGAAAGTTTAGGGTTACATTGACATAATATTCAAATTTATACATTGTAGGTCAATTAATTTTTTTAAAAAAGTCGAATTTGCCAAGGACCTATTAGGCACATAATTGATAGTTTAAGGATTTATCAGACATTTTTAACTGTATGAAATCCATTTGATATATTTGAAAGTTCAAGACCAATAGATTCAAACATTAAAATTTGAAGTTTATGGACTAAATTTACAGTCTAACCAGCTTGTTTGTAATTTTTTTTATCCATATGATGGATGTGTTTGCTTCTCAGGTCTAAAGTTGCTAATTCTGTATGCCCTACTCTTGATCATCTGTGTGAATCATTTCGGTTTCAGCCTTCTACAAAACCTATGAAGCTAAATGCTTTCTTTTGTTCAGGGAGCTCTTGTGCAGCTAGAAGTTGAGGGTTCTGGGTTCTTATATAGACAAGTTCGGAATATGGTAATTTTTGTTATATCTATCTTTACAGTCAAAAGTCCCTTCTCTGATTGTTTTATTTTTACTTATGCTGGTTGCTAAAATGGCTTTATAGTCCTGGAAGCATAAATTTAAGTTTGTAAAATTGGTTTCATGTAACTCAGGAACTGTAAGAATTTCTCTTGTGTGTTTTCAGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCACTTCCTCCTGAGGTTGTTCCTAAGATTTTGGCATCTCGAGATCGCAGAGAGCTTGCTAAGTATGCTTCGGGCGTTCCTCCCCAGGGACTGTGTCTTGTGAGTATTAATTATAAAGAGGACCATTTAAAGCTTCCATCCGATTGCCCTGGGACTAGTTTTGGTAGGACTCGTAGTGTGAGCAAATGTAAAGTCCCTTTTTATTGAATCAATCTTGCACTTATGCCATGAGAATAATCTGCGTTGCAGGGTGTCCCAACAACTTAGGAGGCCGAGATATTATTAGATAAGCAGTGTAGTTTAGTTAACAAAATTTTGGATCAGTTAGTCTTTGTTAATTTATGATTTAGTATATTTATGTTAGTTTGTTATTTGTTTTTTCTTTTTCTTTTCTGTGGTAAGCCTATAGCCACTCTTAGTACACTGTATTGGAACTCTAGAGACTTTGAACATTATTATTGCAATACTTTGTATTTTAGAAGGATTTCT

mRNA sequence

TAACTATCTTCAATATTTGTATGTTTCTTAGTTCTTAGCTTGATTGTTAAACCCGGTTGATGAGAGATATCAAAATTGAATTTATAGAAAGGTGAGGGCTTCCAAGAGTTTATGCAAATATCATCAACTGAAAGCATGATTCTGTATCCTATAAAAAGAGAGAAATTTAGTCATAGGATATTTTGTTTTCATATATATAATATGTAAAATTTATGAGTTTTGAATTTTCACCCACATATTGTCACCAAAAGGGAAATCTCTGAATTGAAAACTCAACGGATGAGGTGCTGGGCGGTGGGTTCGCCTGTTCTTCCTCCAATTTCCCTTCCCCTTTCACCAATCATTCCATCTCCAAATGGCGCTTCATCTTCCTCCAGTCGTGGATTCCCGAATGCGGAGGATGATGCCTCTTATATCAAAGAGCCTTCTTTCCCATGCTTCAAATGGCGGTTGCTCATCGCTTATGACGGTACTCGATATGCAGGCTGGCAATATCAGAGGTCACCACCCACTATCCAGTGCCTCATTGAAGAAGCCTTGATTGGAGCAACAAAGCTTGAAAGGAAGGATCTTCATTTAGTTGGTGCAAGCAGAACAGATAAAGGAGTACATGCCTGGGGTCAGGTGGCTCACTTTGTTACGCCATTTAACTACGATAGCTTGGAAAGCATTCATGCTGCACTGAATGGCCTTCTTCCTGTGGATATAAGAGTTCGAGAGATGAGTCCTGCTGTACCCGATTTTCATGCTCGGTTTTCTACGTTAAGCAAGGTTTATCACTACAAGATATATAGTGGCACAATCATGGATCCATTTCAGCGTTGCTACTCTTACCACTGTGTTTATAAACTCAACTATGCAGCCATGAAAGAAGCTGCAAATTATTTCGTCGGAAAGCATGATTTTTCTGCTTTTACGAATGCAGCTCATAACGATCGAGTGGTAAATCCAGTGAAGGTCATTTTCCGTTTTGATGTGATAGAAATGGGAGCTCTTGTGCAGCTAGAAGTTGAGGGTTCTGGGTTCTTATATAGACAAGTTCGGAATATGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCACTTCCTCCTGAGGTTGTTCCTAAGATTTTGGCATCTCGAGATCGCAGAGAGCTTGCTAAGTATGCTTCGGGCGTTCCTCCCCAGGGACTGTGTCTTGTGAGTATTAATTATAAAGAGGACCATTTAAAGCTTCCATCCGATTGCCCTGGGACTAGTTTTGGTAGGACTCGTAGTGTGAGCAAATGTAAAGTCCCTTTTTATTGAATCAATCTTGCACTTATGCCATGAGAATAATCTGCGTTGCAGGGTGTCCCAACAACTTAGGAGGCCGAGATATTATTAGATAAGCAGTGTAGTTTAGTTAACAAAATTTTGGATCAGTTAGTCTTTGTTAATTTATGATTTAGTATATTTATGTTAGTTTGTTATTTGTTTTTTCTTTTTCTTTTCTGTGGTAAGCCTATAGCCACTCTTAGTACACTGTATTGGAACTCTAGAGACTTTGAACATTATTATTGCAATACTTTGTATTTTAGAAGGATTTCT

Coding sequence (CDS)

ATGAGGTGCTGGGCGGTGGGTTCGCCTGTTCTTCCTCCAATTTCCCTTCCCCTTTCACCAATCATTCCATCTCCAAATGGCGCTTCATCTTCCTCCAGTCGTGGATTCCCGAATGCGGAGGATGATGCCTCTTATATCAAAGAGCCTTCTTTCCCATGCTTCAAATGGCGGTTGCTCATCGCTTATGACGGTACTCGATATGCAGGCTGGCAATATCAGAGGTCACCACCCACTATCCAGTGCCTCATTGAAGAAGCCTTGATTGGAGCAACAAAGCTTGAAAGGAAGGATCTTCATTTAGTTGGTGCAAGCAGAACAGATAAAGGAGTACATGCCTGGGGTCAGGTGGCTCACTTTGTTACGCCATTTAACTACGATAGCTTGGAAAGCATTCATGCTGCACTGAATGGCCTTCTTCCTGTGGATATAAGAGTTCGAGAGATGAGTCCTGCTGTACCCGATTTTCATGCTCGGTTTTCTACGTTAAGCAAGGTTTATCACTACAAGATATATAGTGGCACAATCATGGATCCATTTCAGCGTTGCTACTCTTACCACTGTGTTTATAAACTCAACTATGCAGCCATGAAAGAAGCTGCAAATTATTTCGTCGGAAAGCATGATTTTTCTGCTTTTACGAATGCAGCTCATAACGATCGAGTGGTAAATCCAGTGAAGGTCATTTTCCGTTTTGATGTGATAGAAATGGGAGCTCTTGTGCAGCTAGAAGTTGAGGGTTCTGGGTTCTTATATAGACAAGTTCGGAATATGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCACTTCCTCCTGAGGTTGTTCCTAAGATTTTGGCATCTCGAGATCGCAGAGAGCTTGCTAAGTATGCTTCGGGCGTTCCTCCCCAGGGACTGTGTCTTGTGAGTATTAATTATAAAGAGGACCATTTAAAGCTTCCATCCGATTGCCCTGGGACTAGTTTTGGTAGGACTCGTAGTGTGAGCAAATGTAAAGTCCCTTTTTATTGA

Protein sequence

MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVTPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQRCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY*

Homology

[Close]

BLAST of CSPI06G00210 vs. ExPASy Swiss-Prot
Match: Q6MEE7 (tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=truA1 PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 4.7e-55
Identity = 109/255 (42.75%), Postives = 163/255 (63.92%), Query Frame = 0


Query: 56  WRLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQ 115
           ++L IAYDGT Y+GWQ Q +  +IQ  I+EAL     L+++ + LVG+ RTD GVHA GQ
Sbjct: 4   YKLTIAYDGTNYSGWQIQPNASSIQQKIQEAL--CILLKKEKVVLVGSGRTDAGVHAKGQ 63

Query: 116 VAHFVTPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTI 175
           VAHF    +Y  L  +H +LNGLLP DIR++ + P  P FH+++S + K YHY ++   +
Sbjct: 64  VAHFHFQ-DYIDLSRLHVSLNGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKV 123

Query: 176 MDPFQRCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVV-NPVKVIFRFDVI 235
           MDPFQR YS+H   K++   +K+AA  F G HDF++F N AH      NPV+ ++R D+ 
Sbjct: 124 MDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDIK 183

Query: 236 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 295
                ++LE EG GFLY+ VRN+VG L+ +       E + +I A+++RR+ +  A   P
Sbjct: 184 PNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAA---P 243

Query: 296 PQGLCLVSINYKEDH 310
           P+GL L+ + Y+ ++
Sbjct: 244 PEGLFLIQVFYENEN 252

[Close]

BLAST of CSPI06G00210 vs. ExPASy Swiss-Prot
Match: B1I1B5 (tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=truA PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.8e-47
Identity = 111/251 (44.22%), Postives = 142/251 (56.57%), Query Frame = 0


Query: 57  RLLIAYDGTRYAGWQYQRSP--PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWG 116
           RL +AYDGT Y G+Q Q     PTIQ  +E  L    +L    L + GASRTD GVHA G
Sbjct: 5   RLTLAYDGTAYHGFQKQSGSGLPTIQETLERCL---AELSGAALKVTGASRTDAGVHARG 64

Query: 117 QVAHFVTPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 176
           QV +FVT       E I AALNG+LP DI   +      DFHAR+S ++K Y Y +Y+  
Sbjct: 65  QVVNFVTGRWGIPTERIPAALNGVLPGDIAALDAREVPADFHARYSAVAKTYSYTLYNHP 124

Query: 177 IMDPFQRCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 236
           +  PFQR YS H    L+  AM+ AA + +G+HDFS F  A     V + V+ + R D+ 
Sbjct: 125 VRSPFQRLYSLHVPRALDLRAMRLAARHLMGRHDFSTFQAAGR--PVQSAVRTLTRADIE 184

Query: 237 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 296
               LV+L     GFLY  VR M G L+++G E L PE +P I+AS DR   A+    VP
Sbjct: 185 AQAPLVRLVFRADGFLYNMVRIMAGTLLEVGLERLDPEALPDIIASGDR---ARAGPTVP 244

Query: 297 PQGLCLVSINY 306
           P GLCL  + Y
Sbjct: 245 PHGLCLEEVEY 247

[Close]

BLAST of CSPI06G00210 vs. ExPASy Swiss-Prot
Match: Q8R7Y7 (tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=truA PE=3 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.2e-46
Identity = 109/248 (43.95%), Postives = 146/248 (58.87%), Query Frame = 0


Query: 58  LLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVA 117
           L+I YDGT Y GWQYQ++  T+Q ++E+A+    K+  + ++L+G+SRTD GVHA  QVA
Sbjct: 2   LIIEYDGTNYHGWQYQKNAITVQEVLEKAI---EKVTGEKVNLIGSSRTDTGVHALYQVA 61

Query: 118 HFVTPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMD 177
           +F T       E +  ALN +LP D+ V +       FHAR+S  SK Y Y I +     
Sbjct: 62  NFKTKTKIPP-EKLPYALNAVLPDDVVVVKAKEVDKSFHARYSARSKRYRYLILNRQFPM 121

Query: 178 PFQRCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMG 237
           P  R Y +H  Y L+  AMK+AA +F G HDFSAF   A  ++ VNPVK IF   V + G
Sbjct: 122 PTMRNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAF--KASGNKKVNPVKTIFDLTVEKEG 181

Query: 238 ALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQG 297
            L+++EVE  GFLY  VR +VG LV +G   +    +P I+ S+DR    K     PPQG
Sbjct: 182 DLIKIEVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDR---TKAGITAPPQG 240

Query: 298 LCLVSINY 306
           L LV I Y
Sbjct: 242 LYLVRIVY 240

[Close]

BLAST of CSPI06G00210 vs. ExPASy Swiss-Prot
Match: A4J151 (tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=truA PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.9e-44
Identity = 103/251 (41.04%), Postives = 145/251 (57.77%), Query Frame = 0


Query: 57  RLLIAYDGTRYAGWQYQRSP--PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWG 116
           +L +AYDGT Y G+Q QR     TIQ  +E+AL   + + +  + ++GA RTD GVHA G
Sbjct: 5   KLTLAYDGTNYHGFQEQRGTGLATIQEALEKAL---STIAQTPIQVIGAGRTDAGVHAQG 64

Query: 117 QVAHFVTPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 176
           QV +F +       E    ALN LLP DI+V +      DFHARFS ++K Y Y IY   
Sbjct: 65  QVVNFRSEKWPVPAEKAPLALNVLLPGDIKVVKAEEVPMDFHARFSAVAKTYRYSIYHHR 124

Query: 177 IMDPFQRCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 236
           +M PF R Y YH   +L+  AM+E A Y +G +DF +F   A    V + ++ I+R D+I
Sbjct: 125 VMSPFHRYYCYHEPRRLDVNAMQEGAAYLLGTYDFKSF--QAQGTPVKDTIRTIYRADII 184

Query: 237 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 296
           E   ++ L + G+GFLY  VR + G L+ IG   + PE + KI+ S++R      A   P
Sbjct: 185 EDAPVINLYLRGNGFLYNMVRIITGTLLNIGFGKIKPEDMVKIIESKNRTLAGTTA---P 244

Query: 297 PQGLCLVSINY 306
           PQGLCL+ + Y
Sbjct: 245 PQGLCLMEVEY 247

[Close]

BLAST of CSPI06G00210 vs. ExPASy Swiss-Prot
Match: Q3A3B5 (tRNA pseudouridine synthase A OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=truA PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.9e-43
Identity = 106/262 (40.46%), Postives = 146/262 (55.73%), Query Frame = 0


Query: 57  RLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQV 116
           RL+I YDGT Y GWQ Q +  ++Q ++EEAL    K+      LV + RTD GVHA G V
Sbjct: 5   RLIIEYDGTPYVGWQRQPNGLSVQQVVEEAL---AKVCGGPHSLVSSGRTDAGVHARGMV 64

Query: 117 AHFVTPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIM 176
           AHFVTP     + +    +N  LP  I VR+ +    DFHAR+S + K Y Y IY   + 
Sbjct: 65  AHFVTPQPL-PMAAYREGVNRFLPAQIAVRDAALMPDDFHARYSAVGKWYRYSIYRSPVR 124

Query: 177 DPFQRCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEM 236
            P    +S+H    L+  AM++AA   VG+HDF+AF  +    R    V+ +F  D+++ 
Sbjct: 125 SPLACRFSWHVRTVLDLQAMRQAARLLVGRHDFAAFRASGCAARTT--VREVFSVDIVDA 184

Query: 237 GALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGV--P 296
              V ++V GSGFL   VR MVG LV++G+ A PPE V  +LA   R++     SG+  P
Sbjct: 185 EEWVYVDVRGSGFLRNMVRIMVGTLVEVGRGARPPEEVGAMLAGGCRKQ-----SGITAP 244

Query: 297 PQGLCLVSINYKEDHLKLPSDC 317
            QGLCL+ + Y       P DC
Sbjct: 245 AQGLCLMEVAY------APQDC 249

[Close]

BLAST of CSPI06G00210 vs. ExPASy TrEMBL
Match: A0A0A0KB67 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.1e-187
Identity = 320/322 (99.38%), Postives = 322/322 (100.00%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60
           MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180
           TPFNYDSLES+HAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180

Query: 181 RCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240
           RCY+YHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300

Query: 301 VSINYKEDHLKLPSDCPGTSFG 323
           VSINYKEDHLKLPSDCPGTSFG
Sbjct: 301 VSINYKEDHLKLPSDCPGTSFG 322

[Close]

BLAST of CSPI06G00210 vs. ExPASy TrEMBL
Match: A0A1S3C7L6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 1.8e-187
Identity = 320/335 (95.52%), Postives = 327/335 (97.61%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60
           MRCWAVGSPVLPPISLP SPIIPSPNGASSSSSRGFPNAE D+SYI EP FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAE-DSSYITEPPFPSFKWRLLI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180
           TPFNYDSLES+HAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GTIMDPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQ 180

Query: 181 RCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240
           RCY+YHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMGALV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCL 300

Query: 301 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 336
           VSINYKEDHLKLPSDCPGTS GR+RSV KCKVPFY
Sbjct: 301 VSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

[Close]

BLAST of CSPI06G00210 vs. ExPASy TrEMBL
Match: A0A5D3BY40 (Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00780 PE=3 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 6.4e-180
Identity = 309/322 (95.96%), Postives = 315/322 (97.83%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60
           MRCWAVGSPVLPPISLP SPIIPSPNGASSSSSRGFPNAE D+SYI EP FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAE-DSSYITEPPFPSFKWRLLI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180
           TPFNYDSLES+HAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GTIMDPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQ 180

Query: 181 RCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240
           RCY+YHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMGALV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCL 300

Query: 301 VSINYKEDHLKLPSDCPGTSFG 323
           VSINYKEDHLKLPSDCPGTS G
Sbjct: 301 VSINYKEDHLKLPSDCPGTSLG 321

[Close]

BLAST of CSPI06G00210 vs. ExPASy TrEMBL
Match: A0A6J1L0L1 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 2.1e-167
Identity = 284/335 (84.78%), Postives = 306/335 (91.34%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60
           M CWAVGS VLP +  PLSP IPS  G  SS+ RGFPN+E D+S + EP FP FKWRL+I
Sbjct: 1   MSCWAVGSAVLPSVPRPLSPSIPS-RGTLSSTGRGFPNSE-DSSCVPEPDFPSFKWRLVI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120
           AYDGTRYAGWQYQRSPPTIQCLIEEAL GAT+LERKDLHLVGASRTDKGVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAHFI 120

Query: 121 TPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180
           TPFNYDSL+ IHAALNGLLP DIRVRE+SPAVPDFHARFSTLSKVYHYKIY+  I+DPFQ
Sbjct: 121 TPFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQ 180

Query: 181 RCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240
           RCY+YHCVYKLNYAAMKEAANYFVGKHDFSAF N AHNDRVVNPVKVIFRFDVIEMGAL+
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALI 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMV LL+QIGKEA+PP+VVPKILASRDR+ LAKY + +PPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGLCL 300

Query: 301 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 336
           V INYKEDHLKLPSDCPGTSFGR+ SVSKCK+PFY
Sbjct: 301 VRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 333

[Close]

BLAST of CSPI06G00210 vs. ExPASy TrEMBL
Match: A0A6J1L2M8 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.8e-166
Identity = 284/337 (84.27%), Postives = 306/337 (90.80%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFP--NAEDDASYIKEPSFPCFKWRL 60
           M CWAVGS VLP +  PLSP IPS  G  SS+ RGFP  N+E D+S + EP FP FKWRL
Sbjct: 1   MSCWAVGSAVLPSVPRPLSPSIPS-RGTLSSTGRGFPVMNSE-DSSCVPEPDFPSFKWRL 60

Query: 61  LIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAH 120
           +IAYDGTRYAGWQYQRSPPTIQCLIEEAL GAT+LERKDLHLVGASRTDKGVHAWGQVAH
Sbjct: 61  VIAYDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAH 120

Query: 121 FVTPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDP 180
           F+TPFNYDSL+ IHAALNGLLP DIRVRE+SPAVPDFHARFSTLSKVYHYKIY+  I+DP
Sbjct: 121 FITPFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDP 180

Query: 181 FQRCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGA 240
           FQRCY+YHCVYKLNYAAMKEAANYFVGKHDFSAF N AHNDRVVNPVKVIFRFDVIEMGA
Sbjct: 181 FQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGA 240

Query: 241 LVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGL 300
           L+QLEVEGSGFLYRQVRNMV LL+QIGKEA+PP+VVPKILASRDR+ LAKY + +PPQGL
Sbjct: 241 LIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGL 300

Query: 301 CLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 336
           CLV INYKEDHLKLPSDCPGTSFGR+ SVSKCK+PFY
Sbjct: 301 CLVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 335

[Close]

BLAST of CSPI06G00210 vs. NCBI nr
Match: XP_004138518.2 (uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical protein Csa_005280 [Cucumis sativus])

HSP 1 Score: 693.7 bits (1789), Expect = 7.7e-196
Identity = 333/335 (99.40%), Postives = 335/335 (100.00%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60
           MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI
Sbjct: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140

Query: 121 TPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180
           TPFNYDSLES+HAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ
Sbjct: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200

Query: 181 RCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240
           RCY+YHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV
Sbjct: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL
Sbjct: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320

Query: 301 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 336
           VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY
Sbjct: 321 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 355

[Close]

BLAST of CSPI06G00210 vs. NCBI nr
Match: XP_008458279.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo])

HSP 1 Score: 664.8 bits (1714), Expect = 3.8e-187
Identity = 320/335 (95.52%), Postives = 327/335 (97.61%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60
           MRCWAVGSPVLPPISLP SPIIPSPNGASSSSSRGFPNAE D+SYI EP FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAE-DSSYITEPPFPSFKWRLLI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180
           TPFNYDSLES+HAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GTIMDPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQ 180

Query: 181 RCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240
           RCY+YHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMGALV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCL 300

Query: 301 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 336
           VSINYKEDHLKLPSDCPGTS GR+RSV KCKVPFY
Sbjct: 301 VSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

[Close]

BLAST of CSPI06G00210 vs. NCBI nr
Match: XP_038875516.1 (tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 643.7 bits (1659), Expect = 9.1e-181
Identity = 309/335 (92.24%), Postives = 321/335 (95.82%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60
           MRCWAVGSPVLPPISLPLSP IP PNGASSSSSRG PNAE D+S I  P FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAE-DSSCIIAPPFPSFKWRLLI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFI 120

Query: 121 TPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180
           TPFNYDSLES+HAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIY+G I+DPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQ 180

Query: 181 RCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240
           RCY+YHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI+MG LV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIDMGPLV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILAS+DRRELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCL 300

Query: 301 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 336
           VSINYKEDHLKLPSDCPGTS GR RSVSKCK+PFY
Sbjct: 301 VSINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 334

[Close]

BLAST of CSPI06G00210 vs. NCBI nr
Match: TYK02989.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4 [Cucumis melo var. makuwa])

HSP 1 Score: 639.8 bits (1649), Expect = 1.3e-179
Identity = 309/322 (95.96%), Postives = 315/322 (97.83%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60
           MRCWAVGSPVLPPISLP SPIIPSPNGASSSSSRGFPNAE D+SYI EP FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAE-DSSYITEPPFPSFKWRLLI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180
           TPFNYDSLES+HAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GTIMDPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQ 180

Query: 181 RCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240
           RCY+YHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMGALV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCL 300

Query: 301 VSINYKEDHLKLPSDCPGTSFG 323
           VSINYKEDHLKLPSDCPGTS G
Sbjct: 301 VSINYKEDHLKLPSDCPGTSLG 321

[Close]

BLAST of CSPI06G00210 vs. NCBI nr
Match: XP_038875517.1 (tRNA pseudouridine synthase A 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 618.2 bits (1593), Expect = 4.1e-173
Identity = 300/335 (89.55%), Postives = 311/335 (92.84%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60
           MRCWAVGSPVLPPISLPLSP IP PNGASSSSSRG PNAE D+S I  P FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAE-DSSCIIAPPFPSFKWRLLI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFI 120

Query: 121 TPFNYDSLESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180
           TPFNYDSLES+HAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIY+G I+DPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQ 180

Query: 181 RCYSYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240
           RCY+YHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVK          G LV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVK----------GPLV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILAS+DRRELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCL 300

Query: 301 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 336
           VSINYKEDHLKLPSDCPGTS GR RSVSKCK+PFY
Sbjct: 301 VSINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 324

[Close]

BLAST of CSPI06G00210 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 431.0 bits (1107), Expect = 8.6e-121
Identity = 213/329 (64.74%), Postives = 251/329 (76.29%), Query Frame = 0


Query: 8   SPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLIAYDGTRY 67
           +P LPP SL  +  + SP   S ++        D  +Y         KWRL+IAYDGTR+
Sbjct: 5   TPALPP-SLFTNGYLSSPQNISMTNLNSSVKVSDSGAY---------KWRLVIAYDGTRF 64

Query: 68  AGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVTPFNYDS 127
           AGWQYQ SPPTIQ ++E+ALI  T+L RK+L L+GA RTD GVHAWGQVAHFVTPFNY S
Sbjct: 65  AGWQYQESPPTIQSMLEKALIQITELGRKELQLIGAGRTDAGVHAWGQVAHFVTPFNYTS 124

Query: 128 LESIHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQRCYSYHC 187
           L+S HAALNGLLP DIRVRE+S AVP+FHARFS  SKVY Y+IY+ T MDPFQR ++YHC
Sbjct: 125 LDSFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDTFMDPFQRHWAYHC 184

Query: 188 VYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALVQLEVEGS 247
            YKLN + M+EAAN FVGKHDFSAF NA   D V +P+K I RFDVI+MG+L+QLEVEGS
Sbjct: 185 AYKLNASKMREAANLFVGKHDFSAFANATREDGVPDPLKTISRFDVIQMGSLLQLEVEGS 244

Query: 248 GFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYAS-GVPPQGLCLVSINYK 307
           GFLYRQVRNMV LL+QIGKEAL  ++VP IL ++DRR LAKY S    P GLCLVS+ YK
Sbjct: 245 GFLYRQVRNMVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSLPASPHGLCLVSVKYK 304

Query: 308 EDHLKLPSDCPGTSFGRTRSVSKCKVPFY 336
           EDHLKLP DCP TS GR  +++KCK+PFY
Sbjct: 305 EDHLKLPLDCPVTSSGRHHTITKCKLPFY 323

[Close]

BLAST of CSPI06G00210 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 79.3 bits (194), Expect = 6.4e-15
Identity = 79/265 (29.81%), Postives = 114/265 (43.02%), Query Frame = 0


Query: 58  LLIAYDGTRYAGWQYQ-RSPPTIQCLIEEALIGATKL--ERKDLHLVGASRTDKGVHAWG 117
           L I Y G R+ G+  + +  P+I+  + +AL     L  ++KD       RTDKGV + G
Sbjct: 94  LKIMYFGKRFYGFSAEAQMEPSIESEVFKALERTRLLVGDKKDSCYSRCGRTDKGVSSTG 153

Query: 118 QV------AHFVTPFNYDSLESIHA------------ALNGLLPVDIRVREMSPAVPDFH 177
           QV      +   +P   DS   ++              LN  LP DIRV   SPA  DFH
Sbjct: 154 QVIALFLRSRLKSPPG-DSKAQVNGRTGERPEYDYVRVLNRALPDDIRVIGWSPAPIDFH 213

Query: 178 ARFSTLSKVYHYKIYSGTIMDPFQRCYSYHCVYKLNYAAMKEAANYFVGKHDFSAF---- 237
           ARFS  ++ Y Y  +                   LN +AM  A   F+G+HDF  F    
Sbjct: 214 ARFSCYAREYKYFFWR----------------QNLNLSAMDFAGKKFIGEHDFRNFCKMD 273

Query: 238 -TNAAHNDRVVNPVKVIFRFDVIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPP 295
             N     R V   +V    +  E   L    + GS FL+ Q+R MV +L  IG+     
Sbjct: 274 VANVHCYTRRVTFFEVSPCQNSHEGDQLCTFTMRGSAFLWHQIRCMVAVLFMIGQGVESV 333

The following BLAST results are available for this feature:

BLAST of CSPI06G00210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6MEE7	4.7e-55	42.75	tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=...	[more]
B1I1B5	2.8e-47	44.22	tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477...	[more]
Q8R7Y7	5.2e-46	43.95	tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongen...	[more]
A4J151	2.9e-44	41.04	tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=3491...	[more]
Q3A3B5	1.9e-43	40.46	tRNA pseudouridine synthase A OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC...	[more]

BLAST of CSPI06G00210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KB67	1.1e-187	99.38	tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1	[more]
A0A1S3C7L6	1.8e-187	95.52	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1	[more]
A0A5D3BY40	6.4e-180	95.96	Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A6J1L0L1	2.1e-167	84.78	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=...	[more]
A0A6J1L2M8	6.8e-166	84.27	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=...	[more]

BLAST of CSPI06G00210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138518.2	7.7e-196	99.40	uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical ...	[more]
XP_008458279.1	3.8e-187	95.52	PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo]	[more]
XP_038875516.1	9.1e-181	92.24	tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida]	[more]
TYK02989.1	1.3e-179	95.96	protein TRIGALACTOSYLDIACYLGLYCEROL 4 [Cucumis melo var. makuwa]	[more]
XP_038875517.1	4.1e-173	89.55	tRNA pseudouridine synthase A 1 isoform X2 [Benincasa hispida]	[more]

BLAST of CSPI06G00210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06950.1	8.6e-121	64.74	Pseudouridine synthase family protein	[more]
AT1G34150.1	6.4e-15	29.81	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 52..158 e-value: 1.2E-25 score: 90.0
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 15..157 e-value: 5.2E-40 score: 138.4
None	No IPR available	PANTHER	PTHR11142:SF0	TRNA PSEUDOURIDINE SYNTHASE-LIKE 1	coord: 2..172
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 2..172
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 5..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G00210.1	CSPI06G00210.1	mRNA
CSPI06G00210.2	CSPI06G00210.2	mRNA
CSPI06G00210.3	CSPI06G00210.3	mRNA
CSPI06G00210.4	CSPI06G00210.4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034196	acylglycerol transport
biological_process	GO:1990052	ER to chloroplast lipid transport
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0008033	tRNA processing
biological_process	GO:0009451	RNA modification
molecular_function	GO:0070300	phosphatidic acid binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0106029	tRNA pseudouridine synthase activity
molecular_function	GO:0009982	pseudouridine synthase activity