CSPI06G00090 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G00090
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Pectin lyase-like superfamily protein
Location	Chr6: 67459 .. 70077 (+)
RNA-Seq Expression	CSPI06G00090
Synteny	CSPI06G00090

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACTCAATGGGGTTTTCATTGTAATGATTTGGGTTGTAGCCATTTTGTTTGTGGAGAATTCAAGTAATGTAAATGGAAGATACCATTTTCATAAGGGTAAAAAAGATGAAGATTCCTCTGTTTCTTCACCACCAGAAAATGCAGTTCCTTCAGCTCCAGCTCAGCCACTTCCTTCAGTTCCATCTGATCCTTATCCTAATGATCCTGGAACTGGAAATTCTACTTCTGATTGTGTTTTTGATGTTACTGATTTTGGAGCTGTTGGCGATGGCTGTACTGATGATACTGCTGCCTTTAAGGCTGCATGGAAAGCAGCTTGTGCTGTTGAATCTGCTACTCTTTTAGTTCCTTCCAATCTTTGCTTTAAAATCACTTCAACTATCTTCTCAGGTCCCTGCAAACCTGGACTTGTGTTTAAGGTTTGTAATTCTATATAAGTTTTGAATTTCTCAAGTAATATTTCAGTCCTAATGCAGCCTAGACTACATTATATCCATCTCCACGCATCCTTCAATTAACATTTGCTACATGTCAACTTGGAATTACAAATAGATAGGTGTAGCTCAAAATGTACTTAAGTACTTTTTTGTTTGTAATGAGCAAAAATTCGTTCCAAACAAAAGTGTCTCTCTGTTTGAAATGAGTGAAAGTTCATTCTAGAACAATTTTTATATGAAAGTTTGGACTTATGAGTGAAAATGAAACGAAACAGGTGGACGGGACTTTGATGCCACCAGATGGACCAGAGTCGTGGCCAAAAGCAGATAGTCCAAGGCAATGGCTTGTGTTTTACAGGCTGGATCAAATGACATTGACCGGAAGTGGAACCATTGAAGGGAATGGGCAGAAATGGTGGGAATTGCCATGCAAACCACATAGGGTACCTACATTTGCATTTATTTTCACTTCATCATTTCCTTTGTTATTGAATGTCTCTCCAATTTTCAATCCAAGGCTTAAAATTTCCTTTTTCAGGGTCCAAATGGGTCAACTCTCCCAGGACCATGTGACAGCCCTGCAGTAAGAAAAAACGCATCTTTGCTCTTTAATATTTCTCTCTTCATTTACTATCTATATAAATCAGAAATGCTGACTGAATTTTGGTTTTCTGGTCTTTTTCAGTTGATAAGATTCTTCATGAGCAGTAATTTGGCAGTAAATAGTTTGAGAATCCAAAACAGTCCAATGTTCCATATGAAATTTGATGGGTGTGAAGGAGTTCTGATAGAAAAGCTGTCGATTTCTTCCCCAAAGCTCAGTCCCAATACTGATGGAATCCACATAGAGAATACCAAAGGGGTTGGAATATACAACTCCATGATATCCAATGGTATGAAACAATGGTTTAGTCTTGCCTACTGATTTTCAATTGTTATGAAATCGAGAACCAAAGATACTACAGATATCGATACAAAGATTCACATACTTGGCTAACAAGATAAGAGAAAGAAATATCATATTACAAATTTCTAAAAAGTCAATAGAGTTAGAGTTATATGGTTAATCCCTCTAAACCTTAGGAAAAAAATAAAAATAACATAAATACAAATAAGATTTCGAATAAGAAAAAACTGATCAACACATACCAACACTAATTATCATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAAATACAGGTGATGACTGCATTTCAATTGGGCCTGGTTGTGCCAATGTGGCCATTGAAGGGGTTACTTGTGGGCCTAGTCACGGAATTAGGTACCAACAATGCCAATTCAATTTCTCTTGAATGTGAAATTTTCAATCTACAGTTATTTTTTTATCACCACTCAAACAGAGAGTAATCCGTTGTTCTATGTTTGATTTTGGGAAAGAGCAGCATTGGGAGCTTAGGAGTTCACAATTCTCAAGCATGTGTTTCCAACATCACAGTAAGAAATGCAGTGATTAGAGATTCAGACAACGGATTGAGGATAAAAACATGGCAAGGAGGGTCGGGATCAGTGAGCGACATCTTGTTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAATACTACTGCTTATCAAAGGATTGTCTAAATCAAACATCAGCTGTGTTCGTAAATCAGGTGTTGTACAAGAACATCAAAGGAACTTACGACGTAAGGAACACTCCAATTCACTTCGCTTGCAGCGACTCAGTGGCTTGCACAAATATTACAATGTCTGAAGTAGAGCTGCTGCCACATGAAGGAGAGTTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAAGAGACATTGACCATTCCTCCTATTGATTGCTTGCAAGAGGGAGAACCTCAGAATGTTGCTGAGACTTCAGAATATAGCTGCTGAAAGTGTTTCAGTGCAAACTCATAATAAGAATAATAATACTGTATACAATCCTCCCCACGGCGGAACCACCCAAGAGCCATTTTTGTAAATGGGTCTTGTTTGTTATGGCTTAGGCTCCAACTCCATCCCTTTCTGGGTTCATTTTTTTATCCTCCCATATTGAGTGATGTGATGCCCTGTTGTAAAATTTAAATAGAGATGTGGTGCTATTGGTTTTGAACCGTGTATCTTGTAATTTATAATTTATACACCTAAATGGACCTTTATGTTA

mRNA sequence

ATGGAACTCAATGGGGTTTTCATTGTAATGATTTGGGTTGTAGCCATTTTGTTTGTGGAGAATTCAAGTAATGTAAATGGAAGATACCATTTTCATAAGGGTAAAAAAGATGAAGATTCCTCTGTTTCTTCACCACCAGAAAATGCAGTTCCTTCAGCTCCAGCTCAGCCACTTCCTTCAGTTCCATCTGATCCTTATCCTAATGATCCTGGAACTGGAAATTCTACTTCTGATTGTGTTTTTGATGTTACTGATTTTGGAGCTGTTGGCGATGGCTGTACTGATGATACTGCTGCCTTTAAGGCTGCATGGAAAGCAGCTTGTGCTGTTGAATCTGCTACTCTTTTAGTTCCTTCCAATCTTTGCTTTAAAATCACTTCAACTATCTTCTCAGGTCCCTGCAAACCTGGACTTGTGTTTAAGGTGGACGGGACTTTGATGCCACCAGATGGACCAGAGTCGTGGCCAAAAGCAGATAGTCCAAGGCAATGGCTTGTGTTTTACAGGCTGGATCAAATGACATTGACCGGAAGTGGAACCATTGAAGGGAATGGGCAGAAATGGTGGGAATTGCCATGCAAACCACATAGGGGTCCAAATGGGTCAACTCTCCCAGGACCATGTGACAGCCCTGCATTGATAAGATTCTTCATGAGCAGTAATTTGGCAGTAAATAGTTTGAGAATCCAAAACAGTCCAATGTTCCATATGAAATTTGATGGGTGTGAAGGAGTTCTGATAGAAAAGCTGTCGATTTCTTCCCCAAAGCTCAGTCCCAATACTGATGGAATCCACATAGAGAATACCAAAGGGGTTGGAATATACAACTCCATGATATCCAATGGTGATGACTGCATTTCAATTGGGCCTGGTTGTGCCAATGTGGCCATTGAAGGGGTTACTTGTGGGCCTAGTCACGGAATTAGCATTGGGAGCTTAGGAGTTCACAATTCTCAAGCATGTGTTTCCAACATCACAGTAAGAAATGCAGTGATTAGAGATTCAGACAACGGATTGAGGATAAAAACATGGCAAGGAGGGTCGGGATCAGTGAGCGACATCTTGTTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAATACTACTGCTTATCAAAGGATTGTCTAAATCAAACATCAGCTGTGTTCGTAAATCAGGTGTTGTACAAGAACATCAAAGGAACTTACGACGTAAGGAACACTCCAATTCACTTCGCTTGCAGCGACTCAGTGGCTTGCACAAATATTACAATGTCTGAAGTAGAGCTGCTGCCACATGAAGGAGAGTTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAAGAGACATTGACCATTCCTCCTATTGATTGCTTGCAAGAGGGAGAACCTCAGAATGTTGCTGAGACTTCAGAATATAGCTGCTGAAAGTGTTTCAGTGCAAACTCATAATAAGAATAATAATACTGTATACAATCCTCCCCACGGCGGAACCACCCAAGAGCCATTTTTGTAAATGGGTCTTGTTTGTTATGGCTTAGGCTCCAACTCCATCCCTTTCTGGGTTCATTTTTTTATCCTCCCATATTGAGTGATGTGATGCCCTGTTGTAAAATTTAAATAGAGATGTGGTGCTATTGGTTTTGAACCGTGTATCTTGTAATTTATAATTTATACACCTAAATGGACCTTTATGTTA

Coding sequence (CDS)

ATGGAACTCAATGGGGTTTTCATTGTAATGATTTGGGTTGTAGCCATTTTGTTTGTGGAGAATTCAAGTAATGTAAATGGAAGATACCATTTTCATAAGGGTAAAAAAGATGAAGATTCCTCTGTTTCTTCACCACCAGAAAATGCAGTTCCTTCAGCTCCAGCTCAGCCACTTCCTTCAGTTCCATCTGATCCTTATCCTAATGATCCTGGAACTGGAAATTCTACTTCTGATTGTGTTTTTGATGTTACTGATTTTGGAGCTGTTGGCGATGGCTGTACTGATGATACTGCTGCCTTTAAGGCTGCATGGAAAGCAGCTTGTGCTGTTGAATCTGCTACTCTTTTAGTTCCTTCCAATCTTTGCTTTAAAATCACTTCAACTATCTTCTCAGGTCCCTGCAAACCTGGACTTGTGTTTAAGGTGGACGGGACTTTGATGCCACCAGATGGACCAGAGTCGTGGCCAAAAGCAGATAGTCCAAGGCAATGGCTTGTGTTTTACAGGCTGGATCAAATGACATTGACCGGAAGTGGAACCATTGAAGGGAATGGGCAGAAATGGTGGGAATTGCCATGCAAACCACATAGGGGTCCAAATGGGTCAACTCTCCCAGGACCATGTGACAGCCCTGCATTGATAAGATTCTTCATGAGCAGTAATTTGGCAGTAAATAGTTTGAGAATCCAAAACAGTCCAATGTTCCATATGAAATTTGATGGGTGTGAAGGAGTTCTGATAGAAAAGCTGTCGATTTCTTCCCCAAAGCTCAGTCCCAATACTGATGGAATCCACATAGAGAATACCAAAGGGGTTGGAATATACAACTCCATGATATCCAATGGTGATGACTGCATTTCAATTGGGCCTGGTTGTGCCAATGTGGCCATTGAAGGGGTTACTTGTGGGCCTAGTCACGGAATTAGCATTGGGAGCTTAGGAGTTCACAATTCTCAAGCATGTGTTTCCAACATCACAGTAAGAAATGCAGTGATTAGAGATTCAGACAACGGATTGAGGATAAAAACATGGCAAGGAGGGTCGGGATCAGTGAGCGACATCTTGTTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAATACTACTGCTTATCAAAGGATTGTCTAAATCAAACATCAGCTGTGTTCGTAAATCAGGTGTTGTACAAGAACATCAAAGGAACTTACGACGTAAGGAACACTCCAATTCACTTCGCTTGCAGCGACTCAGTGGCTTGCACAAATATTACAATGTCTGAAGTAGAGCTGCTGCCACATGAAGGAGAGTTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAAGAGACATTGACCATTCCTCCTATTGATTGCTTGCAAGAGGGAGAACCTCAGAATGTTGCTGAGACTTCAGAATATAGCTGCTGA

Protein sequence

MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSAPAQPLPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC*

Homology

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BLAST of CSPI06G00090 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 4.4e-200
Identity = 336/475 (70.74%), Postives = 384/475 (80.84%), Query Frame = 0


Query: 3   LNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSAPAQPLPSVP 62
           L+G+ ++ I +  +LF   ++    RYH+HKG+      +  PP    P   A P   VP
Sbjct: 4   LSGILVIYI-ISTVLFGHFTNVARARYHYHKGRHGVTHPLPPPP--PPPLETANPPDQVP 63

Query: 63  SDPYPN-DPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNL 122
           SDPYP+ DP  G+S S CVFDVT FGAVGDG  DDTAAF+ AWKAACAVES  +L P   
Sbjct: 64  SDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGG 123

Query: 123 CFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTI 182
            FKITSTIFSGPCKPGLVF++DG LMPPDGPE WP+ D+  QWLVFYRLD  T +G GT+
Sbjct: 124 VFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTV 183

Query: 183 EGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDG 242
           EGNGQKWW+LPCKPHRGP+GS+  GPC SP +IRFFMS+N+ V  LRIQNSP FHMKFDG
Sbjct: 184 EGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDG 243

Query: 243 CEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVT 302
           C+GVLI ++ ISSPKLSPNTDGIH+ NT+ VGIYNS++SNGDDCISIG GC++V I+GVT
Sbjct: 244 CQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVT 303

Query: 303 CGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQM 362
           CGPSHGISIGSLGVHNSQACVSNITVRN VIRDSDNGLR+KTWQGG+GSVS++LFENIQM
Sbjct: 304 CGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQM 363

Query: 363 ENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNI 422
           ENV NCIIVDQYYC SKDC N+TSAV V  V Y+NIKGTYDVR+ PIHFACSD+VACTNI
Sbjct: 364 ENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNI 423

Query: 423 TMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEP-QNVAETSEYSC 476
           TMSEVELLP EGELV+DPFCWNA+G QETLTIPPIDCL +G P    A  S Y C
Sbjct: 424 TMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

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BLAST of CSPI06G00090 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 2.5e-86
Identity = 167/388 (43.04%), Postives = 230/388 (59.28%), Query Frame = 0


Query: 77  SDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESAT-LLVPSNLCFKITSTIFSGPCK 136
           S+ +  V +FGA G+G TDDT AF  AWK AC+ +  T +LVP N    +     SGPCK
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCK 108

Query: 137 PGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKP 196
             L  ++ GT++ P+ P+ W +  + R+WL F+ L ++T+ G GT+ G GQ+WWE  CK 
Sbjct: 109 ARLTLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK- 168

Query: 197 HRGPNGSTLPGPC-DSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSISS 256
           H   N      PC  +P  + F    N+ V +L + +S   H+    C  V I  L + +
Sbjct: 169 HNHSN------PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIA 228

Query: 257 PKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLG 316
           P  SPNTDGIHI  ++G+ I N+ +S GDDCISI      ++I  + CGP HGISIGSLG
Sbjct: 229 PATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLG 288

Query: 317 VHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYY 376
              S   V +ITV  A+I D+ NG+RIKTWQGGSG VS I+F NI+M NV N II+DQYY
Sbjct: 289 KSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYY 348

Query: 377 CLS-KDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEG 436
           C S K C NQTSA+ +  + + +++GT       I  +CSDS  C NI + +++L P  G
Sbjct: 349 CDSRKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNG 408

Query: 437 ELVEDPFCWNAFGIQETLTIPPIDCLQE 462
           +   + FCW A+G       PP  CL +
Sbjct: 409 DGFTESFCWEAYGSSSGQVYPP-PCLSD 426

[Close]

BLAST of CSPI06G00090 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.6e-85
Identity = 165/357 (46.22%), Postives = 218/357 (61.06%), Query Frame = 0


Query: 80  VFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLV 139
           +F+V  +GAVGDG  D T AF  AW+AAC   SA LLVP N  F + +  F+GPC+P   
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 140 FKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGP 199
           FKVDG +     P SW    + R WL F +L   TL G G I+G G++WW   CK     
Sbjct: 118 FKVDGIIAAYQNPASW---KNNRIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQCK---WV 177

Query: 200 NGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSP 259
           NG  +    D P  I+F  S+ L +  L++ NSP FH+ F  CEGV I  +SI++P+ SP
Sbjct: 178 NGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSP 237

Query: 260 NTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQ 319
           NTDGI I  +K   +  + I  GDDC++IG G +N+ IE + CGP HGISIGSLG  NS+
Sbjct: 238 NTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSR 297

Query: 320 ACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKD 379
           A VS + V  A   D+ NGLRIKTWQGGSG  S I++EN++M N  N I+++Q+YC S  
Sbjct: 298 AEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSAS 357

Query: 380 -CLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEGEL 436
            C NQ SAV +  V YKNI+GT       I   CSDS+ C +I +S++ L    G++
Sbjct: 358 ACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

[Close]

BLAST of CSPI06G00090 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 6.8e-84
Identity = 167/350 (47.71%), Postives = 212/350 (60.57%), Query Frame = 0


Query: 80  VFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLV 139
           VF+V  +GAVGDG  D T AF   W AAC   SA LLVP+N  F + + +F GPC+P L 
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLS 117

Query: 140 FKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGP 199
           FKVDGT++    P  W  +   + WL F +L    L G+G I+G GQ+WW   CK     
Sbjct: 118 FKVDGTIVAQPDPARWKNS---KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV---V 177

Query: 200 NGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSP 259
           NG T+    + P  I+   S ++ V  L + NSP FH+ F  CEGV I+ L I +P+ SP
Sbjct: 178 NGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSP 237

Query: 260 NTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQ 319
           NTDGI I  +K   I   +I  GDDCI+IG G +N+ I+ + CGP HGISIGSLG  NS+
Sbjct: 238 NTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSR 297

Query: 320 ACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKD 379
           A VS++ V  A   D+ NGLRIKTWQGGSG  S I +EN++M N  N I+++Q+YC S  
Sbjct: 298 AEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSAS 357

Query: 380 -CLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVEL 429
            C NQ SAV +  V YKNI GT       I   CSDSV CT I +S V L
Sbjct: 358 ACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSL 400

[Close]

BLAST of CSPI06G00090 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 3.2e-81
Identity = 166/383 (43.34%), Postives = 226/383 (59.01%), Query Frame = 0


Query: 82  DVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLVFK 141
           +V DFGA GDG  DDT AF+ AWKAAC+  S+ +L+     + +    FSGPCK GL  +
Sbjct: 91  NVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSGLTMQ 150

Query: 142 VDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGPNG 201
           + GT+   D    + K    R WLVF  +  + + G GTI GNG+ WW+  CK ++    
Sbjct: 151 IYGTIEASDDRSDYRK--DGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKAL-- 210

Query: 202 STLPGPC-DSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSPN 261
                PC D+P  + F+ S ++ V +L+I+N+   H+ FD C  V    L +++P+ SPN
Sbjct: 211 -----PCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPN 270

Query: 262 TDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQA 321
           TDGIH+  T+ + I + +I  GDDCISI  G   V +  +TCGP HGISIGSLG  NS+A
Sbjct: 271 TDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEA 330

Query: 322 CVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYC-LSKD 381
            VS++ V  A +  + NG+RIKTWQGGSGS S+I F+N++M NV N II+DQ YC   K 
Sbjct: 331 HVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKP 390

Query: 382 CLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEGELVEDP 441
           C  Q+SAV V  V+Y+NIKGT    N  I F CS    C  I + +V+ L  EG      
Sbjct: 391 CQEQSSAVQVKNVVYQNIKGTC-ASNVAITFDCSKRFPCQGIVLEDVD-LEIEGGAAAKA 450

Query: 442 FCWNAFGIQETLTIPPIDCLQEG 463
            C N   + ET  + P  C +EG
Sbjct: 451 LC-NNVELSETGVVSP-HCQEEG 459

[Close]

BLAST of CSPI06G00090 vs. ExPASy TrEMBL
Match: A0A5D3BVF7 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00640 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.1e-277
Identity = 462/475 (97.26%), Postives = 468/475 (98.53%), Query Frame = 0


Query: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSAPAQPLPS 60
           ME NGV I+MIWV+AILFVENSSNV+GRYHFHKGKKDEDS VSSPPE AVPS PAQ +PS
Sbjct: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTL PPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIE+LSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV
Sbjct: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 476
           ITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 475

[Close]

BLAST of CSPI06G00090 vs. ExPASy TrEMBL
Match: A0A1S3C7J5 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103497761 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.1e-277
Identity = 462/475 (97.26%), Postives = 468/475 (98.53%), Query Frame = 0


Query: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSAPAQPLPS 60
           ME NGV I+MIWV+AILFVENSSNV+GRYHFHKGKKDEDS VSSPPE AVPS PAQ +PS
Sbjct: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTL PPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIE+LSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV
Sbjct: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 476
           ITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 475

[Close]

BLAST of CSPI06G00090 vs. ExPASy TrEMBL
Match: A0A0A0KB54 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000120 PE=3 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 2.1e-269
Identity = 457/475 (96.21%), Postives = 457/475 (96.21%), Query Frame = 0


Query: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSAPAQPLPS 60
           MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPS PAQPLPS
Sbjct: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSTPAQPLPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISN                 GV
Sbjct: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISN-----------------GV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 476
           ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 458

[Close]

BLAST of CSPI06G00090 vs. ExPASy TrEMBL
Match: A0A6J1EWI8 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 PE=3 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 7.2e-254
Identity = 430/484 (88.84%), Postives = 448/484 (92.56%), Query Frame = 0


Query: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKK----DEDSSV-----SSPPENAVP 60
           ME N V  VM+W+ AIL  EN SNV GRYHFHKG+K    DE  S+     S PPE++VP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  SAPAQPLPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 120
           S PA P PSVPSDPYP+DP  GN+TSDCVFDVT++GAVGDGC DDTAAF+AAWKAACAVE
Sbjct: 61  STPADPAPSVPSDPYPDDP--GNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120

Query: 121 SATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLD 180
           SATLLVPS+ CFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADS RQWLVFYRLD
Sbjct: 121 SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSSRQWLVFYRLD 180

Query: 181 QMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQN 240
           QMTLTG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAV+ LRIQN
Sbjct: 181 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQN 240

Query: 241 SPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPG 300
           SP FHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMI NGDDCISIGPG
Sbjct: 241 SPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPG 300

Query: 301 CANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSV 360
           CANV IEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIR+SDNG+RIKTWQGGSG+V
Sbjct: 301 CANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 360

Query: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420
           SDILFENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQVLYKNIKGTYDVRNTPIHFA
Sbjct: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420

Query: 421 CSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETS 476
           CSD+VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEG PQNVAET 
Sbjct: 421 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETY 480

[Close]

BLAST of CSPI06G00090 vs. ExPASy TrEMBL
Match: A0A6J1CB48 (polygalacturonase At1g48100 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009879 PE=3 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.3e-252
Identity = 430/492 (87.40%), Postives = 452/492 (91.87%), Query Frame = 0


Query: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKK----DEDSSV-----SSPPENAVP 60
           ME N V  +++ + AIL VENSSNV GRYHFHKG+K    DED SV     S PPE AVP
Sbjct: 1   MEFNRVLAILVCITAILLVENSSNVQGRYHFHKGQKKQKPDEDPSVPSPVYSPPPEEAVP 60

Query: 61  SA--------PAQPLPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAA 120
           S+        PA+P PSVPSDPYP+DP   NSTSDCVFDVTD+GAVGDGCTDDTAAF+AA
Sbjct: 61  SSPAEPAPSPPAEPAPSVPSDPYPDDP--RNSTSDCVFDVTDYGAVGDGCTDDTAAFRAA 120

Query: 121 WKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQ 180
           WKAACAVESAT+LVPS+ CF ITSTIFSGPCKPGLVFKVDG+LMPPDGPESWPKADS RQ
Sbjct: 121 WKAACAVESATILVPSDHCFIITSTIFSGPCKPGLVFKVDGSLMPPDGPESWPKADSVRQ 180

Query: 181 WLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLA 240
           WLVFYRLDQMT+TG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNL 
Sbjct: 181 WLVFYRLDQMTMTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLV 240

Query: 241 VNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGD 300
           V+ LRIQNSP FHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGD
Sbjct: 241 VSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGD 300

Query: 301 DCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKT 360
           DCISIGPGCANV IEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNG+RIKT
Sbjct: 301 DCISIGPGCANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGVRIKT 360

Query: 361 WQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDV 420
           WQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSK CLNQTSAVFVNQVLY+NIKGTYDV
Sbjct: 361 WQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKGCLNQTSAVFVNQVLYRNIKGTYDV 420

Query: 421 RNTPIHFACSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGE 476
           RNTPIHFACSD+VACTNITMSEVELLPHEGELVEDPFCWNA+GIQETLTIPPIDCLQ+G+
Sbjct: 421 RNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAYGIQETLTIPPIDCLQDGQ 480

[Close]

BLAST of CSPI06G00090 vs. NCBI nr
Match: XP_004138524.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN45612.2 hypothetical protein Csa_005406 [Cucumis sativus])

HSP 1 Score: 983.8 bits (2542), Expect = 5.3e-283
Identity = 474/475 (99.79%), Postives = 474/475 (99.79%), Query Frame = 0


Query: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSAPAQPLPS 60
           MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPS PAQPLPS
Sbjct: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSTPAQPLPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV
Sbjct: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 476
           ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 475

[Close]

BLAST of CSPI06G00090 vs. NCBI nr
Match: XP_008458305.1 (PREDICTED: polygalacturonase At1g48100 [Cucumis melo] >TYK02978.1 polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 964.1 bits (2491), Expect = 4.3e-277
Identity = 462/475 (97.26%), Postives = 468/475 (98.53%), Query Frame = 0


Query: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSAPAQPLPS 60
           ME NGV I+MIWV+AILFVENSSNV+GRYHFHKGKKDEDS VSSPPE AVPS PAQ +PS
Sbjct: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTL PPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIE+LSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV
Sbjct: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 476
           ITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 475

[Close]

BLAST of CSPI06G00090 vs. NCBI nr
Match: XP_038906648.1 (polygalacturonase At1g48100 [Benincasa hispida])

HSP 1 Score: 933.7 bits (2412), Expect = 6.2e-268
Identity = 452/480 (94.17%), Postives = 465/480 (96.88%), Query Frame = 0


Query: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKD----EDSSV-SSPPENAVPSAPA 60
           ME NGVFIVMIW+VA L VENSSNVNGRYHFHKGKK+    EDS V S PPE AVPS+PA
Sbjct: 1   MEFNGVFIVMIWIVATLLVENSSNVNGRYHFHKGKKEEKPNEDSPVFSPPPEKAVPSSPA 60

Query: 61  QPLPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATL 120
           +P+PSVPSDPYPN+P  GNS+SDCVFDVTDFGAVGDGC DDTAAFKAAWKAACAVESATL
Sbjct: 61  EPVPSVPSDPYPNEP--GNSSSDCVFDVTDFGAVGDGCADDTAAFKAAWKAACAVESATL 120

Query: 121 LVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTL 180
           LVPS LCFKITSTIFSGPCKPGLVFKVDGTLMPP+GPESWPKADSPRQWLVFYRLDQMTL
Sbjct: 121 LVPSGLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPESWPKADSPRQWLVFYRLDQMTL 180

Query: 181 TGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMF 240
           TG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMF
Sbjct: 181 TGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMF 240

Query: 241 HMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANV 300
           HMKFDGC+GVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANV
Sbjct: 241 HMKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANV 300

Query: 301 AIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDIL 360
           AI+GVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNG+RIKTWQGGSGSVSDIL
Sbjct: 301 AIDGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGVRIKTWQGGSGSVSDIL 360

Query: 361 FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDS 420
           FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSD+
Sbjct: 361 FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDT 420

Query: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 476
           VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 478

[Close]

BLAST of CSPI06G00090 vs. NCBI nr
Match: XP_022930280.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 885.9 bits (2288), Expect = 1.5e-253
Identity = 430/484 (88.84%), Postives = 448/484 (92.56%), Query Frame = 0


Query: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKK----DEDSSV-----SSPPENAVP 60
           ME N V  VM+W+ AIL  EN SNV GRYHFHKG+K    DE  S+     S PPE++VP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  SAPAQPLPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 120
           S PA P PSVPSDPYP+DP  GN+TSDCVFDVT++GAVGDGC DDTAAF+AAWKAACAVE
Sbjct: 61  STPADPAPSVPSDPYPDDP--GNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120

Query: 121 SATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLD 180
           SATLLVPS+ CFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADS RQWLVFYRLD
Sbjct: 121 SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSSRQWLVFYRLD 180

Query: 181 QMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQN 240
           QMTLTG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAV+ LRIQN
Sbjct: 181 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQN 240

Query: 241 SPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPG 300
           SP FHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMI NGDDCISIGPG
Sbjct: 241 SPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPG 300

Query: 301 CANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSV 360
           CANV IEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIR+SDNG+RIKTWQGGSG+V
Sbjct: 301 CANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 360

Query: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420
           SDILFENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQVLYKNIKGTYDVRNTPIHFA
Sbjct: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420

Query: 421 CSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETS 476
           CSD+VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEG PQNVAET 
Sbjct: 421 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETY 480

[Close]

BLAST of CSPI06G00090 vs. NCBI nr
Match: XP_022138804.1 (polygalacturonase At1g48100 isoform X2 [Momordica charantia])

HSP 1 Score: 880.9 bits (2275), Expect = 4.8e-252
Identity = 430/492 (87.40%), Postives = 452/492 (91.87%), Query Frame = 0


Query: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKK----DEDSSV-----SSPPENAVP 60
           ME N V  +++ + AIL VENSSNV GRYHFHKG+K    DED SV     S PPE AVP
Sbjct: 1   MEFNRVLAILVCITAILLVENSSNVQGRYHFHKGQKKQKPDEDPSVPSPVYSPPPEEAVP 60

Query: 61  SA--------PAQPLPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAA 120
           S+        PA+P PSVPSDPYP+DP   NSTSDCVFDVTD+GAVGDGCTDDTAAF+AA
Sbjct: 61  SSPAEPAPSPPAEPAPSVPSDPYPDDP--RNSTSDCVFDVTDYGAVGDGCTDDTAAFRAA 120

Query: 121 WKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQ 180
           WKAACAVESAT+LVPS+ CF ITSTIFSGPCKPGLVFKVDG+LMPPDGPESWPKADS RQ
Sbjct: 121 WKAACAVESATILVPSDHCFIITSTIFSGPCKPGLVFKVDGSLMPPDGPESWPKADSVRQ 180

Query: 181 WLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLA 240
           WLVFYRLDQMT+TG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNL 
Sbjct: 181 WLVFYRLDQMTMTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLV 240

Query: 241 VNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGD 300
           V+ LRIQNSP FHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGD
Sbjct: 241 VSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGD 300

Query: 301 DCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKT 360
           DCISIGPGCANV IEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNG+RIKT
Sbjct: 301 DCISIGPGCANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGVRIKT 360

Query: 361 WQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDV 420
           WQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSK CLNQTSAVFVNQVLY+NIKGTYDV
Sbjct: 361 WQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKGCLNQTSAVFVNQVLYRNIKGTYDV 420

Query: 421 RNTPIHFACSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGE 476
           RNTPIHFACSD+VACTNITMSEVELLPHEGELVEDPFCWNA+GIQETLTIPPIDCLQ+G+
Sbjct: 421 RNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAYGIQETLTIPPIDCLQDGQ 480

[Close]

BLAST of CSPI06G00090 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 698.7 bits (1802), Expect = 3.1e-201
Identity = 336/475 (70.74%), Postives = 384/475 (80.84%), Query Frame = 0


Query: 3   LNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSAPAQPLPSVP 62
           L+G+ ++ I +  +LF   ++    RYH+HKG+      +  PP    P   A P   VP
Sbjct: 4   LSGILVIYI-ISTVLFGHFTNVARARYHYHKGRHGVTHPLPPPP--PPPLETANPPDQVP 63

Query: 63  SDPYPN-DPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNL 122
           SDPYP+ DP  G+S S CVFDVT FGAVGDG  DDTAAF+ AWKAACAVES  +L P   
Sbjct: 64  SDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGG 123

Query: 123 CFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTI 182
            FKITSTIFSGPCKPGLVF++DG LMPPDGPE WP+ D+  QWLVFYRLD  T +G GT+
Sbjct: 124 VFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTV 183

Query: 183 EGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDG 242
           EGNGQKWW+LPCKPHRGP+GS+  GPC SP +IRFFMS+N+ V  LRIQNSP FHMKFDG
Sbjct: 184 EGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDG 243

Query: 243 CEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVT 302
           C+GVLI ++ ISSPKLSPNTDGIH+ NT+ VGIYNS++SNGDDCISIG GC++V I+GVT
Sbjct: 244 CQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVT 303

Query: 303 CGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQM 362
           CGPSHGISIGSLGVHNSQACVSNITVRN VIRDSDNGLR+KTWQGG+GSVS++LFENIQM
Sbjct: 304 CGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQM 363

Query: 363 ENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNI 422
           ENV NCIIVDQYYC SKDC N+TSAV V  V Y+NIKGTYDVR+ PIHFACSD+VACTNI
Sbjct: 364 ENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNI 423

Query: 423 TMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEP-QNVAETSEYSC 476
           TMSEVELLP EGELV+DPFCWNA+G QETLTIPPIDCL +G P    A  S Y C
Sbjct: 424 TMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

[Close]

BLAST of CSPI06G00090 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 546.2 bits (1406), Expect = 2.6e-155
Identity = 265/447 (59.28%), Postives = 330/447 (73.83%), Query Frame = 0


Query: 26  NGRYHFHKGKKDEDSSVSSPPENAVPSAPAQPLPSVPSDPYPNDPGTGNSTSDCVFDVTD 85
           N   H     K   SS+S PP    P  P  P PS+P  P  +DP   N+    +++V  
Sbjct: 39  NNHNHHSSKPKPPSSSISQPP-TPPPGPPDSPAPSLPPSP-SDDPADDNNG---IYNVRK 98

Query: 86  FGAVGDGCTDDTAAFKAAWKAACAVESAT---LLVPSNLCFKITSTIFSGPCKPGLVFKV 145
           +GAVGDG TDDT AFK AW ++C  E+ T   LLVP    F I STIF+GPC+    F+V
Sbjct: 99  YGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLVPYGYTFMIQSTIFTGPCRSYQFFQV 158

Query: 146 DGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGPNGS 205
           DGT++ PDGPESWP   S RQWLVFYR++ M L G G I+G GQKWW+LPCKPHR  N S
Sbjct: 159 DGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEGVIDGRGQKWWDLPCKPHRSVNKS 218

Query: 206 TL-PGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSPNT 265
            +  GPCDSP  +RFFMSSNL V  L+I+NSP FH +FDGC+GV +E L I++P LSPNT
Sbjct: 219 AIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRFDGCQGVHVESLHITAPPLSPNT 278

Query: 266 DGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGP-SHGISIGSLGVHNSQA 325
           DGIHIEN+  V IYNS+ISNGDDC+SIG G  +V I  +TCGP  HGISIGSLG HNS+A
Sbjct: 279 DGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDVDIRNLTCGPGGHGISIGSLGNHNSRA 338

Query: 326 CVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKDC 385
           CVSNITVR++VI+ SDNG+RIKTWQGGSGSVS + F NI +++VRN II+DQYYC++KDC
Sbjct: 339 CVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVTFNNIHVDSVRNPIIIDQYYCMTKDC 398

Query: 386 LNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEGELVEDPF 445
            N+TSAVFV+ + Y+ IKGTYD+R+ P+HF CSD+V CTN+T+S++ELLP +GE+V DPF
Sbjct: 399 ANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDAVPCTNLTLSDIELLPAKGEIVLDPF 458

Query: 446 CWNAFGIQETLTIPPIDCLQEGEPQNV 468
           CWNA+GI E L+IPP+ CL    P+ +
Sbjct: 459 CWNAYGIAEELSIPPVWCLMSDPPKGL 480

[Close]

BLAST of CSPI06G00090 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 512.7 bits (1319), Expect = 3.2e-145
Identity = 242/409 (59.17%), Postives = 304/409 (74.33%), Query Frame = 0


Query: 59  PSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAAC---AVESATL 118
           PS   +P  ND    ++ +  VFDVT FGA+GD  TDDT+AFK AW AAC     +SA L
Sbjct: 29  PSPAPNPAYND---NDNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALL 88

Query: 119 LVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTL 178
           LVP   CF +  T F+GPC+  LV ++DG ++ PDGP SWP ++  RQW++FYR++ +++
Sbjct: 89  LVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWP-SNYQRQWMMFYRVNGLSI 148

Query: 179 TGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMF 238
            GSG I G GQKWW LPCKPH+G NG+T  GPCDSP  IR F SS + +  +   NS  F
Sbjct: 149 QGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQF 208

Query: 239 HMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANV 298
           H++FD C  V+++ + I +P  SPNTDGIHIENT  V I NSMISNGDDCISIG GC NV
Sbjct: 209 HVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNV 268

Query: 299 AIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDIL 358
            I+ VTCGPSHGISIGSLGVHNSQA VSNITV N+ I +SDNG+RIKTWQGGSGSVS I+
Sbjct: 269 DIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIV 328

Query: 359 FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDS 418
           F NI M NVRN I++DQYYC + +C NQTSAV ++ VLY NIKGTYD+R+ PIHF CSDS
Sbjct: 329 FSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDS 388

Query: 419 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEP 465
           V CTN+T++EV+L P +G+ +E+PFCWNA+G  + +T+PP+ CL +  P
Sbjct: 389 VPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

[Close]

BLAST of CSPI06G00090 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 493.4 bits (1269), Expect = 2.0e-139
Identity = 234/413 (56.66%), Postives = 302/413 (73.12%), Query Frame = 0


Query: 59  PSVPSDPYPNDPGTGNSTS-----DCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE-- 118
           P  PS P PNDP    S S     + V+DV  +GAVG+G  DDT +FK AW +AC+    
Sbjct: 39  PPSPS-PNPNDPSKSPSRSQDLDHEVVYDVRKYGAVGNGVADDTVSFKTAWDSACSNNKN 98

Query: 119 --SATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYR 178
             ++ L VP    F I STIF+GPC+    F+VDGT++P DGP+SWP + + RQWL FYR
Sbjct: 99  NTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYR 158

Query: 179 LDQMTLTGSGTIEGNGQKWWELPCKPH-RGPNGSTLPGPCDSPALIRFFMSSNLAVNSLR 238
           ++ M L G+G I+G GQ WW+LPCKPH +  N + L GPC+SPA +RFFMSSN+ V  L 
Sbjct: 159 INGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAALRFFMSSNVIVKGLS 218

Query: 239 IQNSPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISI 298
           I+NSP  H+K DGC  V I  L I SP  SPNTDGIHIEN+  V IYNS+ISNGDDC+SI
Sbjct: 219 IKNSPQVHLKLDGCHVVHINSLRIISPPASPNTDGIHIENSNSVEIYNSVISNGDDCVSI 278

Query: 299 GPGCANVAIEGVTCGP-SHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGG 358
           GPG  ++ I  +TCGP  HGISIGSLG  NS ACVSN+TVR++ I+ S+NG+RIKTWQGG
Sbjct: 279 GPGAYDIDIRNITCGPGGHGISIGSLGEKNSHACVSNVTVRDSFIKFSENGVRIKTWQGG 338

Query: 359 SGSVSDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTP 418
           SGSVS + F+NI ++ VRN II+DQYYC +K C N+TSAVFVN ++Y++IKGTYD+R+ P
Sbjct: 339 SGSVSGVTFDNIHVDTVRNPIIIDQYYCTTKSCANKTSAVFVNDIVYQSIKGTYDIRSPP 398

Query: 419 IHFACSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQ 461
           +HF CS++V CTN+T+S +ELLP + ++V  PFCWNA+GI +  ++P I CL+
Sbjct: 399 MHFGCSNNVPCTNLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSVPLISCLK 450

[Close]

BLAST of CSPI06G00090 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 371.7 bits (953), Expect = 8.8e-103
Identity = 207/483 (42.86%), Postives = 283/483 (58.59%), Query Frame = 0


Query: 23  SNVNGRYHF-HKGK----------KDEDSS--VSSPPENAVPSAP-AQPLPSVPSDPYPN 82
           S+ N  +H  HK K          K  D S  VS PP+   P  P  QP    P  P P 
Sbjct: 59  SHENNHHHMSHKSKPKPKMKTQPPKSNDGSPVVSQPPQVQQPPPPHVQP----PPTPLPL 118

Query: 83  DPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITST 142
            P   +      F+V DFGA GDG +DDT AF+AAW +AC VE++T+++P +  F +   
Sbjct: 119 QPVEDSQQ----FNVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFLVGPI 178

Query: 143 IFSGP-CKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQK 202
            FSGP C+  +VF+++G ++ P   ESW        W+ F +L  +T+ G+G I+G G  
Sbjct: 179 SFSGPYCQANIVFQLEGMIVAPTDTESW--GGGLMWWIEFTKLSGITIQGNGVIDGRGTV 238

Query: 203 WW-------------------------ELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNL 262
           WW                         E P  P R      +P     P  +RF+ S ++
Sbjct: 239 WWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPS--IKPTALRFYGSIDV 298

Query: 263 AVNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNG 322
            V  + IQNSP  H+KFD C  VL+  +++SSP  SPNTDGIH++NT+ V I+ + ++ G
Sbjct: 299 TVTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACG 358

Query: 323 DDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIK 382
           DDCISI  GC+NV +  V CGP HGISIGSLG  +++ACVSNITVR+ V+ ++  G+RIK
Sbjct: 359 DDCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIK 418

Query: 383 TWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYD 442
           TWQGG GSV  ILF NIQ+  V+  I++DQ+YC    C+N TSAV V  V Y+ I+GTY 
Sbjct: 419 TWQGGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYT 478

Query: 443 VRNTPIHFACSDSVACTNITMSEVELLPHEGEL-VEDPFCWNAFGIQETLTIPPIDCLQE 465
           V+  P+HFACSDS  C ++ +S +EL P + +  + DPFCW  FG   + T+PPIDCLQ 
Sbjct: 479 VK--PVHFACSDSFPCIDVQLSGIELKPVQLQYHMYDPFCWKTFGELNSATVPPIDCLQI 527

The following BLAST results are available for this feature:

BLAST of CSPI06G00090 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949Z1	4.4e-200	70.74	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
Q94AJ5	2.5e-86	43.04	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
P43212	1.6e-85	46.22	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]
Q7M1E7	6.8e-84	47.71	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P35336	3.2e-81	43.34	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1	[more]

BLAST of CSPI06G00090 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BVF7	2.1e-277	97.26	Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G006...	[more]
A0A1S3C7J5	2.1e-277	97.26	polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103497761 PE=3 SV=1	[more]
A0A0A0KB54	2.1e-269	96.21	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000120 PE=3 SV=1	[more]
A0A6J1EWI8	7.2e-254	88.84	polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 P...	[more]
A0A6J1CB48	2.3e-252	87.40	polygalacturonase At1g48100 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of CSPI06G00090 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138524.1	5.3e-283	99.79	polygalacturonase At1g48100 [Cucumis sativus] >KGN45612.2 hypothetical protein C...	[more]
XP_008458305.1	4.3e-277	97.26	PREDICTED: polygalacturonase At1g48100 [Cucumis melo] >TYK02978.1 polygalacturon...	[more]
XP_038906648.1	6.2e-268	94.17	polygalacturonase At1g48100 [Benincasa hispida]	[more]
XP_022930280.1	1.5e-253	88.84	polygalacturonase At1g48100-like [Cucurbita moschata]	[more]
XP_022138804.1	4.8e-252	87.40	polygalacturonase At1g48100 isoform X2 [Momordica charantia]	[more]

BLAST of CSPI06G00090 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48100.1	3.1e-201	70.74	Pectin lyase-like superfamily protein	[more]
AT1G02460.1	2.6e-155	59.28	Pectin lyase-like superfamily protein	[more]
AT1G56710.1	3.2e-145	59.17	Pectin lyase-like superfamily protein	[more]
AT4G01890.1	2.0e-139	56.66	Pectin lyase-like superfamily protein	[more]
AT1G60590.1	8.8e-103	42.86	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 269..290 e-value: 2800.0 score: 4.1 coord: 242..268 e-value: 520.0 score: 9.2 coord: 292..312 e-value: 5300.0 score: 2.1 coord: 322..343 e-value: 6.7 score: 15.7 coord: 351..372 e-value: 390.0 score: 9.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 71..473 e-value: 5.2E-119 score: 399.9
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 112..428 e-value: 4.1E-85 score: 285.7
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 299..312
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 50..66
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 34..74
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 6..466
None	No IPR available	PANTHER	PTHR31375:SF142	POLYGALACTURONASE PLANT-LIKE PROTEIN	coord: 6..466
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 79..473

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G00090.1	CSPI06G00090.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0004650	polygalacturonase activity