CSPI05G30890 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G30890
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionTVP38/TMEM64 family membrane protein
LocationChr5: 28634135 .. 28638971 (+)
RNA-Seq ExpressionCSPI05G30890
SyntenyCSPI05G30890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAAAGAAAATAATAATAATAATAATAAGAGAAGAAGAAGAAAAAAATGTTGTAGAAAAATTGAGTTTCTTCCTCTTCGTTATCACTGCTCTGCTCTTATCTGAGTGAGCTCCATTGATGAGGTGATCAGCTTTCACACAATTTCTTCTCCTCCCTTTTTTTTTTGTTTTGTTTTGTTTATAATGCGAGTCCTTCTGACCTTAAGGCCGGCATCTTCAAACCCACCCCCATGTCTTTCTTCTCCCAATTTCTCTCCTCCTCTTGCCTCTTCTTTCCTCTTCAGTTTCAGACCCAACAAGCGCTTTCACTTCCTCAAGCCTTGTTCCTCTCTCAAGCAATCCAAGAAACCTACTCTTCAGAAGACACCCACTAGCGCCCCTCAAAGCTTTAAGTGGCTTTTTAGCGCCAAGTCCGACGATGACGATGCCGGTGAGAAAGGGAATAAAGGTGTGGATGGAGATGGAGCTGTTTTGGAAGATGACTCTGCGGTTAAGGGCACCCTTTTGGCTGGTGTGTTGCTTGTTGGGGTTATTGGTGGATTTGCATTTGCTGGATATGTGTATAGGGATCCGATTAACGCCTTCTTGAATCAGTTCTCGACCTTTATTGATGGTAATTTTATTATGGTTTATTTGCTTCTTTGTGTGTGTGTGTGTGTGTGTATTTTGAACTGGGTAATGAATTTCTTGGTGTAATGATCAATTTCATTGTGGTTCGATGAGCATTTTGCTGGGGGTGTGTTGTTCATCGCGTTTTGAAATTGGTCTGGATTATGCGTTGGTTATTGTAATGTGAGGCTGTTCTGAAATTCCTCGTGCTGATTCTGATTGTGTGTGTGTGTGGCAATCATATATTGCTCCTCCATCTTCTTCTTCTATGTCTCGTGAAATTTTGATGCAAATTAACCTCAATTTTTGCGAAAATTTGAGTTTTTTAGATTCCCCTTTTAAAAAATTTAACTCTAAAATGCCTGGAGTTAATTTGACATTCCTACTGCTATGCAAAAAAGGGGGAAAACAGCTGAACGTCTTGAATTTCCATAGTTAATAGAGTACTTATATTCTACTTTTCTCTTGTACTTTCATATTTATTCTTTTTTGGTATTAGCTTTCAGACCTTCTGTTTTAGTCCTAGGGGGTAGCGCTCCATTGTAGGTTTAAATTGTTGACCTTGTTAGTTAGACTCTCGGTTGCTTTGGTTGGGTATGAAGTGACTCAATTCCCCTCCACTTATTTAGAGCTCCCGTTTGGAGGGAATTCAAGAACTCAGTTGTTTTGGAATCCTGCTCTGGAAAGTTTTAGAAAAGGTTGTCTTCTTTGAAAAAGACTTACTTTTTCTAAAGGAGGGAGACTCTCTTGTTCAGGTTTTGACGGGGTTCCTAGTTATTTTTTCCTTATTTAGGATTTGGTCTCAGTCTGTAAGAATCTGAGAGATCGATCATTGATGAGAAACTTTTAGTGGCAAGGGGTTGATGAGGGAGAACTTAATGGTTGTGGTGGTCCTTCACTGAAGTCTGAGACTGACGCACTGCGGTGTAGAATTTTTGCAAGTAGTATTTAATGTCATAGGCCATATCCTCCTAAACATCTTGGATAACTACTTATTACTTTGACCACTGAGTTGTCTCATAGTTAAACGTGGCTCTGTTTAAACATAATGGATTAACTGATCAGTAATATGTTATCACCTCTCTACATTTGCAGCTATCTACTTTCTAATTCTAGTTCCCAAATTCTAATAATGGTGTCATTCACTTTAAAGTGCGTGTGGTACTATAGTTGTATTCCTTTGTTTTTAAGCATAAAATCATTACCAGAGAAAAAGGGATATTTTGAATAATTGAGGAGCTGCCATTTCTCTGAAGGATCTCTCTCAATGATGTAATTTTTAACGTAGAGGTTTTGATTAACAGGATATGGTCCAGCAGGATATGCTTTATTTGTAGCAGTATATGCAGGACTGGAGGTACAAATCTGTTTTATTTAATACAAATATTTTGGAAATTTACCTTCTACATTTATAGTGGTGCCCTTTGTTGTAAGGCATGGCATATTTGGGGTCGCTGTAGGGGTGACCCAGTTGGAGAACTTAATAGATTAAAACCTTTGTAGCTTTTTGTCTACTTCGACCCTTTGGGATCAAATGGTGATGGGGCTATGAACATAATGTAAGAATTGGACTTGAAGTAGACCAAAAGTACTATTAGATTTATAACATATATGCTGAAAGCCTGAAACTATGTTTTCCTGTACTAGTTCTACTTGTAGTTGTTGTACCACTTTTTGGTTGCCCATGTGGAGGTGCAAGAGATACTTCCTCCATGGTTTAGTTTTTTACTTTTGCCAACTATCGATTATGAAGTTTATGTTATGATTTCTTTCCTTCTAGATCCTTGCAATTCCCGCCATTCCATTAACAATGTCAGCTGGACTCCTCTTTGGTTCTGTAATTGGGACTGTTATAGTTTCCATCAGTGGAACGGTGAGTGTTCTGTACCAATATGCAAATAGATAGATTATCATGTGCCTCCTAGTTATTAACTTTGTATCTCTTATTAATTCAGGTTGCTGCTAGTGTTGCCTTTTTGATTGCTCGGTATTTTGCTCGTGAGCGTATTCTTAAGCTAGTAGAAGGAAACAAGAAATTTGCTGCCATTGACAAAGCCATTGGAGAAAATGGTTTCAAAGTTGTTACCCTCCTACGTTTGAGCCCATTGCTTCCATTTTCTCTAGGAAATTATTTATATGGACTCACATCTGTCAAATTTGTCCCCTATGTGTTGGGAAGGTCAGTTTTCAATTCCTCAATGAATTTCAAAATTGTACCTCTTGCCCTGTTTCATTTCTATAGTGATATCTATATTCATAAATGATTGTAGCTTTCTTTTTCTTCAATAAACTATTTTGATTCTCCTTAGTTTTATTGATACATACAGAATGCTATGAGCTGTATGAACTTCAAAACATAAACAGATTTATTTTCTTTAATGCAGCTGGCTGGGGATGCTTCCAGGAACATGGGCATATGTGAGTGCGGGTGCCTTTGGACGTGCAATTATTGTAAGTTTTCAACCCGTTGCCCCTCACTACTTAGAGATGTGATAATAACATCTTACCTCATAAATGTGTACTTGACTTGGTTCTTAAAGTGTTCGTGCTTGTGCTTTTTAGGACCGATTGCTTCTTTATTTAAAATTTCTATTAGTAAAAGGTGCATTTGACCAATTATCAGTGCTTTTTAAAGTCTTTCTAGAATGCACTTGGGGTGCATTTGGGATTGATTTTGTAGGAGTTTAAAGTGATTATGAGGAAATTTTAACTGTTTAGTGTTTGGTAAAACTGATTACAGGATAATTGAAAGGAAATCACTTTTTCAATTCAATTGGTTTCTAAAATCTATGTAGAGTAGTTTTTGAGATTGAGTTCCAAAAATATCATGCCTCTTCTAATTAACTACTAAAATTCATAGTTTATTCCTATTCTATAATACTTTTTCTCATTTAAGTTTGAAATTTACCAAAACACTTGTTTACTTCATTCTACAGCCGATATTTTAAAAAGTGCAGCTATTAGTAATTATTTCAAAAGCTACAATAACTAAGCCTTAAAGTGCTCTTTTGTGAAAGCATTTACACTTTTATTAAGAGGTAAGACAAACACATCAATGTGTTTGCTTGAACTATGATCTGGATTCTTAGTTGGGTTTTCTGTCTCTATTGTTTTGTTTCTAAGTTTTCGAGTTTCAATTTTATGGTCTGGTTTCTTTTCCACTCTTAATTTTACTTGGGGTGTTTCAGCAAGAAGAGTCTGAAGTTAGTTTGCTTGGAGGGGGAAATGGTCAGCTATGGACTTTAGGACTGGGATTGCTGGCAACAGCATTGGCTGCTGCATACGTTACACGATTAGCCAAGGTAATATATCTTATTATTGCTATGCTATACTACAGACGTAATGCAATCAAATCTTCATTTTGGAACCACGATCTAGCCAATATGGAATTAACTCATAATCATGAAATTTACTCTATCATCATCATATTACAGTTTATAGGTCCATAGCCCCAGCTTATTCACATCTCTATCTCGCCATTCTTTTACGAGGGAATGTGGTTAGAGAGTTATGTCGTGGACTTAAAAAGGGATATGAGTATTTGAGAATGTTGGGTCTAAATGTTAGGATCAAATAATATTTACATGATATTAGTGAAAAAATACACACAAATTGGATACATTAAAGATTCCATAGTTGATTGATATTGAAATTTGTTGGTTTAAACAATCATTCTTTAATTTCAGTAATTGAATGGGAGGTGGTACGGACATCAAGGATATTTTGCATAAGATCAGACCGTCATTCTATAATGATTGGTCAGGTTATATATGCAAACAAAGGTGTCCAAAATCAAAAGAGTTACTCTATAAGATTAGTCGGCATACATGCATACAAAGATGTACACAATCAAAGTGTGATTTGATTAGTTGGGATATTATATGCAAACATAGTTATGGGTGGGGTTCTTTGGTTAGATTAATGTGAAAGGGTTGTGAATGTTGTTTTAATTTATTTTTGAAAATTGCAGGATGCTATGAAGGATATTGAATAGGCATGGCACCTCGGTGAATGTAGTAGGAGGTATAAAATTAAATTCATAATTGAATTTTGAAAATTGGCAAATGCTCTGTATATTTTTTGTTTGACCAATTCAGAGAAACTTCAACCAGTTGCTGATAATGTATAATGTTTACATTAATAATCAATATAATCAATCTCTAATTGTAACTTAAACCTCTCTCTCTATCTATCTATAACATAAAAGTATATGATATGAGTTTTTGG

mRNA sequence

AAAGAAAGAAAATAATAATAATAATAATAAGAGAAGAAGAAGAAAAAAATGTTGTAGAAAAATTGAGTTTCTTCCTCTTCGTTATCACTGCTCTGCTCTTATCTGAGTGAGCTCCATTGATGAGGTGATCAGCTTTCACACAATTTCTTCTCCTCCCTTTTTTTTTTGTTTTGTTTTGTTTATAATGCGAGTCCTTCTGACCTTAAGGCCGGCATCTTCAAACCCACCCCCATGTCTTTCTTCTCCCAATTTCTCTCCTCCTCTTGCCTCTTCTTTCCTCTTCAGTTTCAGACCCAACAAGCGCTTTCACTTCCTCAAGCCTTGTTCCTCTCTCAAGCAATCCAAGAAACCTACTCTTCAGAAGACACCCACTAGCGCCCCTCAAAGCTTTAAGTGGCTTTTTAGCGCCAAGTCCGACGATGACGATGCCGGTGAGAAAGGGAATAAAGGTGTGGATGGAGATGGAGCTGTTTTGGAAGATGACTCTGCGGTTAAGGGCACCCTTTTGGCTGGTGTGTTGCTTGTTGGGGTTATTGGTGGATTTGCATTTGCTGGATATGTGTATAGGGATCCGATTAACGCCTTCTTGAATCAGTTCTCGACCTTTATTGATGGATATGGTCCAGCAGGATATGCTTTATTTGTAGCAGTATATGCAGGACTGGAGATCCTTGCAATTCCCGCCATTCCATTAACAATGTCAGCTGGACTCCTCTTTGGTTCTGTAATTGGGACTGTTATAGTTTCCATCAGTGGAACGGTTGCTGCTAGTGTTGCCTTTTTGATTGCTCGGTATTTTGCTCGTGAGCGTATTCTTAAGCTAGTAGAAGGAAACAAGAAATTTGCTGCCATTGACAAAGCCATTGGAGAAAATGGTTTCAAAGTTGTTACCCTCCTACGTTTGAGCCCATTGCTTCCATTTTCTCTAGGAAATTATTTATATGGACTCACATCTGTCAAATTTGTCCCCTATGTGTTGGGAAGCTGGCTGGGGATGCTTCCAGGAACATGGGCATATGTGAGTGCGGGTGCCTTTGGACGTGCAATTATTCAAGAAGAGTCTGAAGTTAGTTTGCTTGGAGGGGGAAATGGTCAGCTATGGACTTTAGGACTGGGATTGCTGGCAACAGCATTGGCTGCTGCATACGTTACACGATTAGCCAAGGATGCTATGAAGGATATTGAATAGGCATGGCACCTCGGTGAATGTAGTAGGAGGTATAAAATTAAATTCATAATTGAATTTTGAAAATTGGCAAATGCTCTGTATATTTTTTGTTTGACCAATTCAGAGAAACTTCAACCAGTTGCTGATAATGTATAATGTTTACATTAATAATCAATATAATCAATCTCTAATTGTAACTTAAACCTCTCTCTCTATCTATCTATAACATAAAAGTATATGATATGAGTTTTTGG

Coding sequence (CDS)

ATGCGAGTCCTTCTGACCTTAAGGCCGGCATCTTCAAACCCACCCCCATGTCTTTCTTCTCCCAATTTCTCTCCTCCTCTTGCCTCTTCTTTCCTCTTCAGTTTCAGACCCAACAAGCGCTTTCACTTCCTCAAGCCTTGTTCCTCTCTCAAGCAATCCAAGAAACCTACTCTTCAGAAGACACCCACTAGCGCCCCTCAAAGCTTTAAGTGGCTTTTTAGCGCCAAGTCCGACGATGACGATGCCGGTGAGAAAGGGAATAAAGGTGTGGATGGAGATGGAGCTGTTTTGGAAGATGACTCTGCGGTTAAGGGCACCCTTTTGGCTGGTGTGTTGCTTGTTGGGGTTATTGGTGGATTTGCATTTGCTGGATATGTGTATAGGGATCCGATTAACGCCTTCTTGAATCAGTTCTCGACCTTTATTGATGGATATGGTCCAGCAGGATATGCTTTATTTGTAGCAGTATATGCAGGACTGGAGATCCTTGCAATTCCCGCCATTCCATTAACAATGTCAGCTGGACTCCTCTTTGGTTCTGTAATTGGGACTGTTATAGTTTCCATCAGTGGAACGGTTGCTGCTAGTGTTGCCTTTTTGATTGCTCGGTATTTTGCTCGTGAGCGTATTCTTAAGCTAGTAGAAGGAAACAAGAAATTTGCTGCCATTGACAAAGCCATTGGAGAAAATGGTTTCAAAGTTGTTACCCTCCTACGTTTGAGCCCATTGCTTCCATTTTCTCTAGGAAATTATTTATATGGACTCACATCTGTCAAATTTGTCCCCTATGTGTTGGGAAGCTGGCTGGGGATGCTTCCAGGAACATGGGCATATGTGAGTGCGGGTGCCTTTGGACGTGCAATTATTCAAGAAGAGTCTGAAGTTAGTTTGCTTGGAGGGGGAAATGGTCAGCTATGGACTTTAGGACTGGGATTGCTGGCAACAGCATTGGCTGCTGCATACGTTACACGATTAGCCAAGGATGCTATGAAGGATATTGAATAG

Protein sequence

MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQKTPTSAPQSFKWLFSAKSDDDDAGEKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVIGGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSLLGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE*
Homology
BLAST of CSPI05G30890 vs. ExPASy Swiss-Prot
Match: Q55909 (TVP38/TMEM64 family membrane protein slr0305 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0305 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.3e-30
Identity = 78/198 (39.39%), Postives = 118/198 (59.60%), Query Frame = 0

Query: 141 FIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFL 200
           +IDG G      F+ +Y    ++ +P   LT+ AG++FG ++G++ V I  T+ A+ AFL
Sbjct: 11  WIDGLGTWAAIAFMLLYTVATVVFLPGSILTLGAGVVFGVILGSIYVFIGATLGATAAFL 70

Query: 201 IARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKF 260
           + RY AR  + K + GN+KF AID+A+G+ G K+V L RLSP+ PF+L NY YG+T+V  
Sbjct: 71  VGRYLARGWVAKKIAGNQKFKAIDEAVGKEGLKIVILTRLSPVFPFNLLNYAYGITNVSL 130

Query: 261 VPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSLLGGGNGQL-----WTLGL-GLLA 320
             YV+GS LGM+PGT  YV  G+           ++ LG    Q      WT+ + G +A
Sbjct: 131 KDYVIGS-LGMIPGTIMYVYIGSLA-------GSLATLGTATNQANPTLQWTIRIVGFIA 190

Query: 321 TALAAAYVTRLAKDAMKD 333
           T     YVT++A+ A+ +
Sbjct: 191 TVAVTIYVTKIARKALNE 200

BLAST of CSPI05G30890 vs. ExPASy Swiss-Prot
Match: P76219 (TVP38/TMEM64 family membrane protein YdjX OS=Escherichia coli (strain K12) OX=83333 GN=ydjX PE=3 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 1.7e-20
Identity = 56/144 (38.89%), Postives = 87/144 (60.42%), Query Frame = 0

Query: 135 LNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVA 194
           L    T I   G  GY+L++ ++    +L +P   L ++ G++FG ++GT++  I+ T+A
Sbjct: 33  LPHLQTLIRQSGFFGYSLYILLFIIATLLLLPGSILVIAGGIVFGPLLGTLLSLIAATLA 92

Query: 195 ASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYG 254
           +S +FL+AR+  R+ +LK V  +  F AI+K I  NG   + L RL PL P+++ NY YG
Sbjct: 93  SSCSFLLARWLGRDLLLKYVGHSNTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYG 152

Query: 255 LTSVKFVPYVLGSWLGMLPGTWAY 279
           LT++ F PY L S L  LPG   Y
Sbjct: 153 LTTIAFWPYTLISALTTLPGIVIY 176

BLAST of CSPI05G30890 vs. ExPASy Swiss-Prot
Match: P9WFS2 (TVP38/TMEM64 family membrane protein MT1538 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1538 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 7.8e-10
Identity = 42/130 (32.31%), Postives = 69/130 (53.08%), Query Frame = 0

Query: 153 FVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILK 212
           F+ V+  + +   P    T++AGLLFGSV+G  I  +  T +A +A L+ R  A    L 
Sbjct: 72  FLLVHTVVTVPPFPRTAFTLAAGLLFGSVVGVFIAVVGSTASAVIAMLLVR--ATGWQLN 131

Query: 213 LVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGML 272
            +   +    +D+ + E G+  +  LRL P++PF+  NY  G + V+ + +   +  G+L
Sbjct: 132 SLVRRRAINRLDERLRERGWLAILSLRLIPVVPFAAINYAAGASGVRILSFAWATLAGLL 191

Query: 273 PGTWAYVSAG 283
           PGT A V  G
Sbjct: 192 PGTAAVVILG 199

BLAST of CSPI05G30890 vs. ExPASy Swiss-Prot
Match: P9WFS3 (TVP38/TMEM64 family membrane protein Rv1491c OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv1491c PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 7.8e-10
Identity = 42/130 (32.31%), Postives = 69/130 (53.08%), Query Frame = 0

Query: 153 FVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILK 212
           F+ V+  + +   P    T++AGLLFGSV+G  I  +  T +A +A L+ R  A    L 
Sbjct: 72  FLLVHTVVTVPPFPRTAFTLAAGLLFGSVVGVFIAVVGSTASAVIAMLLVR--ATGWQLN 131

Query: 213 LVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGML 272
            +   +    +D+ + E G+  +  LRL P++PF+  NY  G + V+ + +   +  G+L
Sbjct: 132 SLVRRRAINRLDERLRERGWLAILSLRLIPVVPFAAINYAAGASGVRILSFAWATLAGLL 191

Query: 273 PGTWAYVSAG 283
           PGT A V  G
Sbjct: 192 PGTAAVVILG 199

BLAST of CSPI05G30890 vs. ExPASy Swiss-Prot
Match: P67118 (TVP38/TMEM64 family membrane protein Mb1528c OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB1528C PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 7.8e-10
Identity = 42/130 (32.31%), Postives = 69/130 (53.08%), Query Frame = 0

Query: 153 FVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILK 212
           F+ V+  + +   P    T++AGLLFGSV+G  I  +  T +A +A L+ R  A    L 
Sbjct: 72  FLLVHTVVTVPPFPRTAFTLAAGLLFGSVVGVFIAVVGSTASAVIAMLLVR--ATGWQLN 131

Query: 213 LVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGML 272
            +   +    +D+ + E G+  +  LRL P++PF+  NY  G + V+ + +   +  G+L
Sbjct: 132 SLVRRRAINRLDERLRERGWLAILSLRLIPVVPFAAINYAAGASGVRILSFAWATLAGLL 191

Query: 273 PGTWAYVSAG 283
           PGT A V  G
Sbjct: 192 PGTAAVVILG 199

BLAST of CSPI05G30890 vs. ExPASy TrEMBL
Match: A0A0A0KST0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G649300 PE=4 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.2e-178
Identity = 333/334 (99.70%), Postives = 333/334 (99.70%), Query Frame = 0

Query: 1   MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60
           MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK
Sbjct: 1   MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60

Query: 61  TPTSAPQSFKWLFSAKSDDDDAGEKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVIGGF 120
           TPTSAPQSFKWLFSAKSDDDDAGEKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVIGGF
Sbjct: 61  TPTSAPQSFKWLFSAKSDDDDAGEKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVIGGF 120

Query: 121 AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS 180
           AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS
Sbjct: 121 AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS 180

Query: 181 VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL 240
           VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL
Sbjct: 181 VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL 240

Query: 241 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSLLGG 300
           SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEV LLGG
Sbjct: 241 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGG 300

Query: 301 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 335
           GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE
Sbjct: 301 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 334

BLAST of CSPI05G30890 vs. ExPASy TrEMBL
Match: A0A5A7T8S8 (TVP38/TMEM64 family membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00810 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.3e-174
Identity = 325/334 (97.31%), Postives = 329/334 (98.50%), Query Frame = 0

Query: 1   MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60
           MRVLLTLRP SSNPPPCLSS NFSPPL+SSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK
Sbjct: 1   MRVLLTLRPPSSNPPPCLSSLNFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60

Query: 61  TPTSAPQSFKWLFSAKSDDDDAGEKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVIGGF 120
           TPTSAPQSFKWLFS KS+DDDAG+KGNKG DGDGAVLEDDSAVKGT+LAGVLLVGVIGGF
Sbjct: 61  TPTSAPQSFKWLFSPKSNDDDAGDKGNKGADGDGAVLEDDSAVKGTILAGVLLVGVIGGF 120

Query: 121 AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS 180
           AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS
Sbjct: 121 AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS 180

Query: 181 VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL 240
           VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL
Sbjct: 181 VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL 240

Query: 241 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSLLGG 300
           SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEV LLGG
Sbjct: 241 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGG 300

Query: 301 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 335
           GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE
Sbjct: 301 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 334

BLAST of CSPI05G30890 vs. ExPASy TrEMBL
Match: A0A1S3CB16 (TVP38/TMEM64 family membrane protein slr0305 OS=Cucumis melo OX=3656 GN=LOC103498671 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.3e-174
Identity = 325/334 (97.31%), Postives = 329/334 (98.50%), Query Frame = 0

Query: 1   MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60
           MRVLLTLRP SSNPPPCLSS NFSPPL+SSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK
Sbjct: 1   MRVLLTLRPPSSNPPPCLSSLNFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60

Query: 61  TPTSAPQSFKWLFSAKSDDDDAGEKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVIGGF 120
           TPTSAPQSFKWLFS KS+DDDAG+KGNKG DGDGAVLEDDSAVKGT+LAGVLLVGVIGGF
Sbjct: 61  TPTSAPQSFKWLFSPKSNDDDAGDKGNKGADGDGAVLEDDSAVKGTILAGVLLVGVIGGF 120

Query: 121 AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS 180
           AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS
Sbjct: 121 AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS 180

Query: 181 VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL 240
           VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL
Sbjct: 181 VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL 240

Query: 241 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSLLGG 300
           SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEV LLGG
Sbjct: 241 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGG 300

Query: 301 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 335
           GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE
Sbjct: 301 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 334

BLAST of CSPI05G30890 vs. ExPASy TrEMBL
Match: A0A6J1I7M0 (uncharacterized protein LOC111472021 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472021 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.7e-164
Identity = 312/337 (92.58%), Postives = 324/337 (96.14%), Query Frame = 0

Query: 1   MRVLLTLRPASSN-PPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQ 60
           MR LLTL P+SSN PPPCLSS +FSPPL+SSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQ
Sbjct: 1   MRALLTLIPSSSNPPPPCLSSLHFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQ 60

Query: 61  KTPTSAPQSFKWLFSAKSDDDDAG--EKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVI 120
           K+PT+APQSFKWLFS KSD+DD G  ++G+K  +GD A LEDD+AVKGTLLAGVLLVGVI
Sbjct: 61  KSPTTAPQSFKWLFSPKSDEDDDGDADEGSKAAEGDQAALEDDTAVKGTLLAGVLLVGVI 120

Query: 121 GGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLL 180
           GGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLL
Sbjct: 121 GGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLL 180

Query: 181 FGSVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTL 240
           FGSVIGT+IVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTL
Sbjct: 181 FGSVIGTIIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTL 240

Query: 241 LRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSL 300
           LRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEV L
Sbjct: 241 LRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGL 300

Query: 301 LGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 335
           LGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE
Sbjct: 301 LGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 337

BLAST of CSPI05G30890 vs. ExPASy TrEMBL
Match: A0A6J1EBL2 (uncharacterized protein LOC111432699 OS=Cucurbita moschata OX=3662 GN=LOC111432699 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.9e-163
Identity = 310/337 (91.99%), Postives = 323/337 (95.85%), Query Frame = 0

Query: 1   MRVLLTLRPASSN-PPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQ 60
           MR  LTL P+SS+ PPPCLSS +FSPPL+SSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQ
Sbjct: 1   MRAFLTLIPSSSSPPPPCLSSLHFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQ 60

Query: 61  KTPTSAPQSFKWLFSAKSDDDDAG--EKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVI 120
           K+PT+APQSFKWLFS KSD+DD G  ++G+KG +GD A LEDD+AVKGTLLAGVLLVGVI
Sbjct: 61  KSPTTAPQSFKWLFSPKSDEDDDGDADEGSKGAEGDQAALEDDTAVKGTLLAGVLLVGVI 120

Query: 121 GGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLL 180
           GGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVA YAGLEILAIPAIPLTMSAGLL
Sbjct: 121 GGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAAYAGLEILAIPAIPLTMSAGLL 180

Query: 181 FGSVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTL 240
           FGS+IGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTL
Sbjct: 181 FGSLIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTL 240

Query: 241 LRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSL 300
           LRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEV L
Sbjct: 241 LRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGL 300

Query: 301 LGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 335
           LGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE
Sbjct: 301 LGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 337

BLAST of CSPI05G30890 vs. NCBI nr
Match: XP_004141545.1 (uncharacterized protein LOC101204460 [Cucumis sativus] >KGN52660.1 hypothetical protein Csa_007947 [Cucumis sativus])

HSP 1 Score: 635.6 bits (1638), Expect = 2.5e-178
Identity = 333/334 (99.70%), Postives = 333/334 (99.70%), Query Frame = 0

Query: 1   MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60
           MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK
Sbjct: 1   MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60

Query: 61  TPTSAPQSFKWLFSAKSDDDDAGEKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVIGGF 120
           TPTSAPQSFKWLFSAKSDDDDAGEKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVIGGF
Sbjct: 61  TPTSAPQSFKWLFSAKSDDDDAGEKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVIGGF 120

Query: 121 AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS 180
           AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS
Sbjct: 121 AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS 180

Query: 181 VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL 240
           VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL
Sbjct: 181 VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL 240

Query: 241 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSLLGG 300
           SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEV LLGG
Sbjct: 241 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGG 300

Query: 301 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 335
           GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE
Sbjct: 301 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 334

BLAST of CSPI05G30890 vs. NCBI nr
Match: XP_008459587.1 (PREDICTED: TVP38/TMEM64 family membrane protein slr0305 [Cucumis melo] >KAA0039288.1 TVP38/TMEM64 family membrane protein [Cucumis melo var. makuwa] >TYK00473.1 TVP38/TMEM64 family membrane protein [Cucumis melo var. makuwa])

HSP 1 Score: 621.3 bits (1601), Expect = 4.8e-174
Identity = 325/334 (97.31%), Postives = 329/334 (98.50%), Query Frame = 0

Query: 1   MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60
           MRVLLTLRP SSNPPPCLSS NFSPPL+SSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK
Sbjct: 1   MRVLLTLRPPSSNPPPCLSSLNFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60

Query: 61  TPTSAPQSFKWLFSAKSDDDDAGEKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVIGGF 120
           TPTSAPQSFKWLFS KS+DDDAG+KGNKG DGDGAVLEDDSAVKGT+LAGVLLVGVIGGF
Sbjct: 61  TPTSAPQSFKWLFSPKSNDDDAGDKGNKGADGDGAVLEDDSAVKGTILAGVLLVGVIGGF 120

Query: 121 AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS 180
           AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS
Sbjct: 121 AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS 180

Query: 181 VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL 240
           VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL
Sbjct: 181 VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL 240

Query: 241 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSLLGG 300
           SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEV LLGG
Sbjct: 241 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGG 300

Query: 301 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 335
           GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE
Sbjct: 301 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 334

BLAST of CSPI05G30890 vs. NCBI nr
Match: XP_038890133.1 (TVP38/TMEM64 family membrane protein slr0305 [Benincasa hispida])

HSP 1 Score: 611.7 bits (1576), Expect = 3.8e-171
Identity = 321/334 (96.11%), Postives = 326/334 (97.60%), Query Frame = 0

Query: 1   MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60
           MR LLTLRP SSNPPPCLSS +FSPPL+SSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK
Sbjct: 1   MRALLTLRPPSSNPPPCLSSLHFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60

Query: 61  TPTSAPQSFKWLFSAKSDDDDAGEKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVIGGF 120
           TPTSAPQSFKWLFS KS+DDDAG+KGNKGVDGDG VLED +AV GTLLAGVLLVGVIGGF
Sbjct: 61  TPTSAPQSFKWLFSPKSNDDDAGDKGNKGVDGDGDVLEDGTAVTGTLLAGVLLVGVIGGF 120

Query: 121 AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS 180
           AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS
Sbjct: 121 AFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGS 180

Query: 181 VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL 240
           VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL
Sbjct: 181 VIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRL 240

Query: 241 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSLLGG 300
           SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEV LLGG
Sbjct: 241 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGG 300

Query: 301 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 335
           GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE
Sbjct: 301 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 334

BLAST of CSPI05G30890 vs. NCBI nr
Match: XP_023535509.1 (uncharacterized protein LOC111796928 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 590.1 bits (1520), Expect = 1.2e-164
Identity = 313/337 (92.88%), Postives = 325/337 (96.44%), Query Frame = 0

Query: 1   MRVLLTLRPASSN-PPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQ 60
           MR LLTL P+SSN PPPCLSS +FSPPL+SSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQ
Sbjct: 1   MRALLTLIPSSSNPPPPCLSSLHFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQ 60

Query: 61  KTPTSAPQSFKWLFSAKSDDDDAG--EKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVI 120
           K+PT+APQSFKWLFS KSD+DD G  ++G+KG +GD A LEDD+AVKGTLLAGVLLVGVI
Sbjct: 61  KSPTTAPQSFKWLFSPKSDEDDDGDVDEGSKGAEGDQAALEDDTAVKGTLLAGVLLVGVI 120

Query: 121 GGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLL 180
           GGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLL
Sbjct: 121 GGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLL 180

Query: 181 FGSVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTL 240
           FGS+IGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTL
Sbjct: 181 FGSLIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTL 240

Query: 241 LRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSL 300
           LRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEV L
Sbjct: 241 LRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGL 300

Query: 301 LGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 335
           LGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE
Sbjct: 301 LGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 337

BLAST of CSPI05G30890 vs. NCBI nr
Match: XP_022973470.1 (uncharacterized protein LOC111472021 isoform X2 [Cucurbita maxima] >XP_022973472.1 uncharacterized protein LOC111472021 isoform X2 [Cucurbita maxima])

HSP 1 Score: 588.6 bits (1516), Expect = 3.5e-164
Identity = 312/337 (92.58%), Postives = 324/337 (96.14%), Query Frame = 0

Query: 1   MRVLLTLRPASSN-PPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQ 60
           MR LLTL P+SSN PPPCLSS +FSPPL+SSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQ
Sbjct: 1   MRALLTLIPSSSNPPPPCLSSLHFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQ 60

Query: 61  KTPTSAPQSFKWLFSAKSDDDDAG--EKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVI 120
           K+PT+APQSFKWLFS KSD+DD G  ++G+K  +GD A LEDD+AVKGTLLAGVLLVGVI
Sbjct: 61  KSPTTAPQSFKWLFSPKSDEDDDGDADEGSKAAEGDQAALEDDTAVKGTLLAGVLLVGVI 120

Query: 121 GGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLL 180
           GGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLL
Sbjct: 121 GGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLL 180

Query: 181 FGSVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTL 240
           FGSVIGT+IVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTL
Sbjct: 181 FGSVIGTIIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTL 240

Query: 241 LRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSL 300
           LRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEV L
Sbjct: 241 LRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGL 300

Query: 301 LGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 335
           LGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE
Sbjct: 301 LGGGNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 337

BLAST of CSPI05G30890 vs. TAIR 10
Match: AT1G22850.1 (SNARE associated Golgi protein family )

HSP 1 Score: 441.4 bits (1134), Expect = 6.4e-124
Identity = 246/333 (73.87%), Postives = 274/333 (82.28%), Query Frame = 0

Query: 5   LTLRPASSNPPPCLSSPNF-SPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQKTPT 64
           LTLR +SS+  P   +  F SPP  SS   SFR  KRFHFLKPCSSLKQ+KK   Q  P+
Sbjct: 8   LTLRSSSSSSLPFTCNSRFCSPPPPSS---SFRLYKRFHFLKPCSSLKQTKKKKQQSLPS 67

Query: 65  SA--PQSFKWLFSAKSDDDDAGEKGNKGVDGDGAVLEDDSAVKGTLLAGVLLVGVIGGFA 124
           +A  PQS +W F++KS +D+  E   K    D    E D+A+KGT+LAGVLL+G +GGFA
Sbjct: 68  TAPPPQSLRWFFNSKSTNDENDEDDVKSESDDDGGSEGDAAIKGTILAGVLLIGTVGGFA 127

Query: 125 FAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSV 184
             GYVYRD IN FL QFST+I+GYG AGYALF+AVYAGLEILAIPA+PLTMSAGLLFG +
Sbjct: 128 GVGYVYRDQINTFLTQFSTYIEGYGTAGYALFIAVYAGLEILAIPALPLTMSAGLLFGPL 187

Query: 185 IGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLS 244
           IGT+IVSISGT+AASVAFLIARYFARERILKLVE NKKF AIDKAIGENGF+VVTLLRLS
Sbjct: 188 IGTIIVSISGTMAASVAFLIARYFARERILKLVEDNKKFLAIDKAIGENGFRVVTLLRLS 247

Query: 245 PLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSLLGGG 304
           PLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPG+WAYVSAGAFGRAIIQEES V  L GG
Sbjct: 248 PLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGSWAYVSAGAFGRAIIQEESNVG-LPGG 307

Query: 305 NGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 335
           NGQL TLG+GLL TALA  YVT LAKDA+KDI+
Sbjct: 308 NGQLLTLGVGLLVTALAGTYVTSLAKDAIKDID 336

BLAST of CSPI05G30890 vs. TAIR 10
Match: AT5G19070.1 (SNARE associated Golgi protein family )

HSP 1 Score: 108.2 bits (269), Expect = 1.3e-23
Identity = 76/229 (33.19%), Postives = 120/229 (52.40%), Query Frame = 0

Query: 105 GTLLAGVLLVGVIGGFAFAGYVYRDPINAFLNQFSTFID-GYGPAGYALFVAVYAGLEIL 164
           G+ L   +L+ ++     A Y    P+   L  F  +++   GP G       Y  L +L
Sbjct: 6   GSALRISVLLILVAAIVLACYFL--PVEKLLKDFLLWVEQDLGPWGPFALAVAYIPLTVL 65

Query: 165 AIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAI 224
           A+PA  LT+  G LFG  IG V  S+  T+ +  AFL+ R   +  ++  ++   +F ++
Sbjct: 66  AVPASVLTLGGGYLFGLPIGFVADSVGATLGSGAAFLLGRTIGKPFVVAKLKDYPQFQSV 125

Query: 225 DKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGA 284
             AI ++GFK+  LLRL+PLLPFS+ NYL  +T ++  PY+L SWLGM+P T A V  G 
Sbjct: 126 ALAIEKSGFKICLLLRLAPLLPFSMLNYLLSVTPIRLGPYLLSSWLGMMPITLALVYVGT 185

Query: 285 FGRAIIQEESEVSLLGGGNGQLWT-LGLGLLATALAAAYVTRLAKDAMK 332
             + +     + S    G    W  L   L+ + +    VT++AKDA++
Sbjct: 186 TLKDLSDVTHKWSEFSPGR---WAFLISSLVISVILMVCVTKVAKDALR 229

BLAST of CSPI05G30890 vs. TAIR 10
Match: AT1G03260.1 (SNARE associated Golgi protein family )

HSP 1 Score: 104.8 bits (260), Expect = 1.4e-22
Identity = 64/173 (36.99%), Postives = 96/173 (55.49%), Query Frame = 0

Query: 111 VLLVGVIGGFAFAGYVYRDPINAFLNQFSTFI-DGYGPAGYALFVAVYAGLEILAIPAIP 170
           +LLV ++    F       P+   L  F  +I +  GP G       Y  L I+A+PA  
Sbjct: 18  LLLVAIVSAVIFL------PVEQKLKDFLLWIKEDLGPFGPLALALAYIPLTIVAVPASV 77

Query: 171 LTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGE 230
           LT+  G LFG  +G V  S+  T+ A+ AFL+ R   +  +   ++   KF A+  AI +
Sbjct: 78  LTLGGGYLFGLPVGFVADSLGATLGATAAFLLGRTIGKSYVTSKIKHYPKFQAVSVAIQK 137

Query: 231 NGFKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAG 283
           +GFK+V LLR+ P+LPF++ NYL  +T V+   Y+L +WLGM+P T+A V  G
Sbjct: 138 SGFKIVLLLRVVPILPFNMLNYLLSVTPVRLGEYMLATWLGMMPITFALVYVG 184

BLAST of CSPI05G30890 vs. TAIR 10
Match: AT4G22850.1 (SNARE associated Golgi protein family )

HSP 1 Score: 63.5 bits (153), Expect = 3.6e-10
Identity = 77/274 (28.10%), Postives = 120/274 (43.80%), Query Frame = 0

Query: 78  DDDDAG---------EKGNKGVDGDGAVLEDDSA----VKGTLLAGVLLVGVIGGFAFAG 137
           DDD+ G         EKG +          D S     VK +LL   L    + G+ + G
Sbjct: 19  DDDEKGPYVKLSEVLEKGQESEQEKEEEKTDSSRFWFWVKLSLLFAFLAALAVVGYIWIG 78

Query: 138 YVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGT 197
            +  D     L Q+      +   G  +F +V A    + +P+ P    AG+ FG   G 
Sbjct: 79  PLIMDKELIPLIQWEIRTFTHPVCGLLVFASV-AIFPTILLPSTPSMWIAGMTFGYGYGF 138

Query: 198 VIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENG----FKVVTLLRL 257
           +++  +  V  S+ + I + F   +I   +E     AA+ +A GE      F +VTL+R+
Sbjct: 139 LLIISAAAVGVSLPYFIGQLFC-HKIQGWLERYPDQAAVLRAAGEGNWLHQFLLVTLIRI 198

Query: 258 SPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVSLLGG 317
           SP  P+ L NY    T VK+ PY+ GS LGM+P  +  +  G   R  + E S     G 
Sbjct: 199 SP-FPYILYNYCSVATRVKYGPYITGSLLGMVPEVFVAIYTGILVRT-LAEASSAEEQGL 258

Query: 318 GNGQLWTLGLGLLATALAAAYVTRLAKDAMKDIE 335
              Q+    LG LAT      +T+ AK  ++ ++
Sbjct: 259 SVTQVILNILGFLATVATTILITKYAKRQLETMK 288

BLAST of CSPI05G30890 vs. TAIR 10
Match: AT2G02370.1 (SNARE associated Golgi protein family )

HSP 1 Score: 62.4 bits (150), Expect = 8.0e-10
Identity = 63/238 (26.47%), Postives = 121/238 (50.84%), Query Frame = 0

Query: 107 LLAGVLLVGVIGGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGL-EILAI 166
           + A  LL+ ++ G     +V++  +   L   +T    +G    A+ + V   L  +  I
Sbjct: 61  ICAVALLLTLVFGKWGVPFVFQKVLIPILQWEAT---AFGRPMLAIVLVVSLALFPVFLI 120

Query: 167 PAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDK 226
           P+ P    AG++FG  +G VI+ +  T+   + +LI   F R+R+ + ++   + AA+ +
Sbjct: 121 PSGPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLMF-RDRLHQWLKRWPRQAAVLR 180

Query: 227 AIGENG----FKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSA 286
              E      F+VV + R+SP  P+++ NY   +TS++F PY  GS  GM+P  + Y+ +
Sbjct: 181 LAAEGSWFHQFRVVAIFRVSP-FPYTIFNYAIVVTSMRFWPYFFGSIAGMIPEAFIYIYS 240

Query: 287 GAFGRAIIQEESEVSLLGGGNGQLWTLGLGLLATALAAAYVTRL-----AKDAMKDIE 335
           G     +I+  ++V     G+ +L T+ +     +L  A VT +     AK A+++++
Sbjct: 241 G----RLIRTFADVQY---GHQRLTTVEIVYNVISLVIAVVTTVAFTVYAKRALRELQ 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q559092.3e-3039.39TVP38/TMEM64 family membrane protein slr0305 OS=Synechocystis sp. (strain PCC 68... [more]
P762191.7e-2038.89TVP38/TMEM64 family membrane protein YdjX OS=Escherichia coli (strain K12) OX=83... [more]
P9WFS27.8e-1032.31TVP38/TMEM64 family membrane protein MT1538 OS=Mycobacterium tuberculosis (strai... [more]
P9WFS37.8e-1032.31TVP38/TMEM64 family membrane protein Rv1491c OS=Mycobacterium tuberculosis (stra... [more]
P671187.8e-1032.31TVP38/TMEM64 family membrane protein Mb1528c OS=Mycobacterium bovis (strain ATCC... [more]
Match NameE-valueIdentityDescription
A0A0A0KST01.2e-17899.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G649300 PE=4 SV=1[more]
A0A5A7T8S82.3e-17497.31TVP38/TMEM64 family membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3CB162.3e-17497.31TVP38/TMEM64 family membrane protein slr0305 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1I7M01.7e-16492.58uncharacterized protein LOC111472021 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1EBL21.9e-16391.99uncharacterized protein LOC111432699 OS=Cucurbita moschata OX=3662 GN=LOC1114326... [more]
Match NameE-valueIdentityDescription
XP_004141545.12.5e-17899.70uncharacterized protein LOC101204460 [Cucumis sativus] >KGN52660.1 hypothetical ... [more]
XP_008459587.14.8e-17497.31PREDICTED: TVP38/TMEM64 family membrane protein slr0305 [Cucumis melo] >KAA00392... [more]
XP_038890133.13.8e-17196.11TVP38/TMEM64 family membrane protein slr0305 [Benincasa hispida][more]
XP_023535509.11.2e-16492.88uncharacterized protein LOC111796928 [Cucurbita pepo subsp. pepo][more]
XP_022973470.13.5e-16492.58uncharacterized protein LOC111472021 isoform X2 [Cucurbita maxima] >XP_022973472... [more]
Match NameE-valueIdentityDescription
AT1G22850.16.4e-12473.87SNARE associated Golgi protein family [more]
AT5G19070.11.3e-2333.19SNARE associated Golgi protein family [more]
AT1G03260.11.4e-2236.99SNARE associated Golgi protein family [more]
AT4G22850.13.6e-1028.10SNARE associated Golgi protein family [more]
AT2G02370.18.0e-1026.47SNARE associated Golgi protein family [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032816SNARE associated Golgi proteinPFAMPF09335SNARE_assoccoord: 165..283
e-value: 1.3E-23
score: 83.7
NoneNo IPR availablePANTHERPTHR46826FAMILY NOT NAMEDcoord: 9..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G30890.1CSPI05G30890.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane