CSPI05G30470 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G30470
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationChr5: 28366422 .. 28367572 (-)
RNA-Seq ExpressionCSPI05G30470
SyntenyCSPI05G30470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCGTCCAGCGTTTGTTACGAACTCTCGACTCCACAGCTTCCATGGAAGCACAAGGTCACTCTTCGATTCGCCACTCTACTCTTCAACACTTCTCTCCGATCAGATTTCACCGTCAATCGCCGGCTTCTCACTTTCCTCGACCCCAAAATCCCTCCCAATCCCAATTCAGCTCACTCCGTTTCCTCTTCCGATCTCACTATCGACACTTCTCGTGACCTCTTCCTTCGAATATTCACTCCCAACCCCACCGCCGCTCTCGATGAATCTCTCCCACTGCTGCCGATCATTTTCTACTTCCATGGCGGCGGCTTCGCCTTCGGTTCTGCCGACGCCACTTCAACTGACATGGCTGCCCGCGGCTTCGCCGAAAAGTTACGCGCCGTCGTAATCTCCGTCAACTACCGCCTCGCTCCTGAGTTTCGATTCCCATGCCAATATGACGATGGGTTCGACGCTTTGAAATTCATTGATGAAATGGACGACGACAGTTTACTAGAGAGGGTAGATTTGAGTCGTTGCTTTATTTTAGGGGAAAGCGCCGGTGGAAATCTAGGACACCATGTGGCAGTGAGAGCGAGCGAATACGAGTTCAAAAGAGTGAAGATCATTGGATTTATAGCGTCGCAGCCGTTTTTCGGCGGAAAGGAAAGAACGGAGTCAGAGAATCGACTGTGTAAGCAACTTCCGTTGACTCTATACATGACGGATTGGTTTTGGAGGGCATTTCTGCCAGCCGGGGAAGATCGAGACCATGCGGGGGCGAATATGAATGGACCCAATGGAAGGGATATCTCGGGGTTGGAAAATTTTCCGGCGACAGTGATTTTCGCGGGAGGATTGGATCTGCTGATGGATCGACAAAAAAGTTATTACGAGAGGTTGAAGAGAATGGGAAAGGATGTGAAGCTGGTGGTGTTTTCCAACGCCTTCCATGGATTCTTTGGCTTCCCAGATCTGCCGGAATATTCTTTGATGATTGAGGAGATGAGTGATTTCATTGCAAAGCTCATGTAGGAAAAATGAAGAATTAACATGTCGTGTGATATCTCTGTCTCCATTCTCATTGTTTATGCTCAAAATCAAAAAGTTGAAGGGACACCATTTCCAATAAAAAATAATCTCTACATCAACAAATTCTCTTAGCA

mRNA sequence

ATGGGTTCGTCCAGCGTTTGTTACGAACTCTCGACTCCACAGCTTCCATGGAAGCACAAGGTCACTCTTCGATTCGCCACTCTACTCTTCAACACTTCTCTCCGATCAGATTTCACCGTCAATCGCCGGCTTCTCACTTTCCTCGACCCCAAAATCCCTCCCAATCCCAATTCAGCTCACTCCGTTTCCTCTTCCGATCTCACTATCGACACTTCTCGTGACCTCTTCCTTCGAATATTCACTCCCAACCCCACCGCCGCTCTCGATGAATCTCTCCCACTGCTGCCGATCATTTTCTACTTCCATGGCGGCGGCTTCGCCTTCGGTTCTGCCGACGCCACTTCAACTGACATGGCTGCCCGCGGCTTCGCCGAAAAGTTACGCGCCGTCGTAATCTCCGTCAACTACCGCCTCGCTCCTGAGTTTCGATTCCCATGCCAATATGACGATGGGTTCGACGCTTTGAAATTCATTGATGAAATGGACGACGACAGTTTACTAGAGAGGGTAGATTTGAGTCGTTGCTTTATTTTAGGGGAAAGCGCCGGTGGAAATCTAGGACACCATGTGGCAGTGAGAGCGAGCGAATACGAGTTCAAAAGAGTGAAGATCATTGGATTTATAGCGTCGCAGCCGTTTTTCGGCGGAAAGGAAAGAACGGAGTCAGAGAATCGACTGTGTAAGCAACTTCCGTTGACTCTATACATGACGGATTGGTTTTGGAGGGCATTTCTGCCAGCCGGGGAAGATCGAGACCATGCGGGGGCGAATATGAATGGACCCAATGGAAGGGATATCTCGGGGTTGGAAAATTTTCCGGCGACAGTGATTTTCGCGGGAGGATTGGATCTGCTGATGGATCGACAAAAAAGTTATTACGAGAGGTTGAAGAGAATGGGAAAGGATGTGAAGCTGGTGGTGTTTTCCAACGCCTTCCATGGATTCTTTGGCTTCCCAGATCTGCCGGAATATTCTTTGATGATTGAGGAGATGAGTGATTTCATTGCAAAGCTCATGTAGGAAAAATGAAGAATTAACATGTCGTGTGATATCTCTGTCTCCATTCTCATTGTTTATGCTCAAAATCAAAAAGTTGAAGGGACACCATTTCCAATAAAAAATAATCTCTACATCAACAAATTCTCTTAGCA

Coding sequence (CDS)

ATGGGTTCGTCCAGCGTTTGTTACGAACTCTCGACTCCACAGCTTCCATGGAAGCACAAGGTCACTCTTCGATTCGCCACTCTACTCTTCAACACTTCTCTCCGATCAGATTTCACCGTCAATCGCCGGCTTCTCACTTTCCTCGACCCCAAAATCCCTCCCAATCCCAATTCAGCTCACTCCGTTTCCTCTTCCGATCTCACTATCGACACTTCTCGTGACCTCTTCCTTCGAATATTCACTCCCAACCCCACCGCCGCTCTCGATGAATCTCTCCCACTGCTGCCGATCATTTTCTACTTCCATGGCGGCGGCTTCGCCTTCGGTTCTGCCGACGCCACTTCAACTGACATGGCTGCCCGCGGCTTCGCCGAAAAGTTACGCGCCGTCGTAATCTCCGTCAACTACCGCCTCGCTCCTGAGTTTCGATTCCCATGCCAATATGACGATGGGTTCGACGCTTTGAAATTCATTGATGAAATGGACGACGACAGTTTACTAGAGAGGGTAGATTTGAGTCGTTGCTTTATTTTAGGGGAAAGCGCCGGTGGAAATCTAGGACACCATGTGGCAGTGAGAGCGAGCGAATACGAGTTCAAAAGAGTGAAGATCATTGGATTTATAGCGTCGCAGCCGTTTTTCGGCGGAAAGGAAAGAACGGAGTCAGAGAATCGACTGTGTAAGCAACTTCCGTTGACTCTATACATGACGGATTGGTTTTGGAGGGCATTTCTGCCAGCCGGGGAAGATCGAGACCATGCGGGGGCGAATATGAATGGACCCAATGGAAGGGATATCTCGGGGTTGGAAAATTTTCCGGCGACAGTGATTTTCGCGGGAGGATTGGATCTGCTGATGGATCGACAAAAAAGTTATTACGAGAGGTTGAAGAGAATGGGAAAGGATGTGAAGCTGGTGGTGTTTTCCAACGCCTTCCATGGATTCTTTGGCTTCCCAGATCTGCCGGAATATTCTTTGATGATTGAGGAGATGAGTGATTTCATTGCAAAGCTCATGTAG

Protein sequence

MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM*
Homology
BLAST of CSPI05G30470 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-78
Identity = 152/324 (46.91%), Postives = 202/324 (62.35%), Query Frame = 0

Query: 15  LPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRD 74
           LP K ++ L   + + + + R D T+NRR L   D + PPNP   + VS+SD  +D SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISV 134
           L+ R++TP+ +         +P++ +FHGGGFAF S +A   D   R FA KL A VISV
Sbjct: 73  LWFRLYTPHVSG------DKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISV 132

Query: 135 NYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGESAGGNLGHHVAV-- 194
           NYRLAPE R+P QYDDGFDALK+I+E     L    DLSRCF  G+SAGGN+ H+VA+  
Sbjct: 133 NYRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRI 192

Query: 195 -RASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDR 254
            R     F  VK+IG I+ QPFFGG+ERTE+E +L     ++   TDW W+A    G +R
Sbjct: 193 CREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNR 252

Query: 255 DHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNA 314
           DH   N+ GPN  DISGL+ +P T++   G D L D Q+SYYE LK  GK   L+ + N 
Sbjct: 253 DHEAVNVGGPNAVDISGLD-YPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNM 312

Query: 315 FHGFFGFPDLPEYSLMIEEMSDFI 336
           FH F+ FP+LPE   +I  + DF+
Sbjct: 313 FHAFYIFPELPEAGQLIMRIKDFV 326

BLAST of CSPI05G30470 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.2e-60
Identity = 134/343 (39.07%), Postives = 191/343 (55.69%), Query Frame = 0

Query: 2   GSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHS 61
           GS  V    S   +P    V +    L +N   R D T NR L  FLD K+P N N  + 
Sbjct: 3   GSEEVNLIESKTVVPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNG 62

Query: 62  VSSSDLTIDTSRDLFLRIFTP----NPTAALDESLP----LLPIIFYFHGGGFAFGSADA 121
           V S D+ ID   +L  R++ P       +  D   P    ++P+I +FHGG FA  SA++
Sbjct: 63  VFSFDVIIDRQTNLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANS 122

Query: 122 TSTDMAARGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLS 181
              D   R       AVV+SVNYR APE R+PC YDDG+  LK+++      L  + D  
Sbjct: 123 AIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVN--SSSWLRSKKDSK 182

Query: 182 -RCFILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPL 241
            R F+ G+S+GGN+ H+VAVRA E    R+ ++G I   P FGG ERTESE RL  +  +
Sbjct: 183 VRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFV 242

Query: 242 TLYMTDWFWRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSY 301
           T+   DW+WRAFLP GEDR+H   +  GP  + + GL +FP +++   GLDL+ D Q  Y
Sbjct: 243 TVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKY 302

Query: 302 YERLKRMGKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFI 336
            E LK+ G++VKL+    A  GF+  P+   +  +++E++ F+
Sbjct: 303 AEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of CSPI05G30470 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.4e-59
Identity = 134/326 (41.10%), Postives = 182/326 (55.83%), Query Frame = 0

Query: 28  LLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTPNPTAA 87
           L +N   R+D T  R L  +LD ++P N      VSS D  ID S  L +RI+       
Sbjct: 29  LSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYRAAAEGD 88

Query: 88  LDE-----SLPLL------------PIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAV 147
            +E     + P+L            P+I +FHGG F   SA +T  D   R F +  + V
Sbjct: 89  AEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGV 148

Query: 148 VISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDL-SRCFILGESAGGNLGHH 207
           V+SVNYR APE R+PC YDDG+ ALK++  M    +    D  +R F+ G+S+GGN+ HH
Sbjct: 149 VVSVNYRRAPEHRYPCAYDDGWTALKWV--MSQPFMRSGGDAQARVFLSGDSSGGNIAHH 208

Query: 208 VAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGE 267
           VAVRA++   + VK+ G I     FGG ERTESE RL  +  +TL   DW+W+A+LP   
Sbjct: 209 VAVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDA 268

Query: 268 DRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFS 327
           DRDH   N  GPNGR + GL  F  ++I   GLDL  DRQ +Y + L+  G  VK+V   
Sbjct: 269 DRDHPACNPFGPNGRRLGGLP-FAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCE 328

Query: 328 NAFHGFFGFPDLPEYSLMIEEMSDFI 336
           NA  GF+  P+   Y  ++EE+SDF+
Sbjct: 329 NATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of CSPI05G30470 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.5e-56
Identity = 121/317 (38.17%), Postives = 179/317 (56.47%), Query Frame = 0

Query: 30  FNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTP------N 89
           +N   R D T NR L  +LD K+  N N    V S D+ ID   +L  R++ P       
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  PTAALDESLP----LLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISVNYRLA 149
           P + LD   P    ++P+I +FHGG FA  SA++   D   R      + VV+SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLS-RCFILGESAGGNLGHHVAVRASEYE 209
           PE  +PC YDDG+ AL +++      L  + D     F+ G+S+GGN+ H+VA+RA E  
Sbjct: 151 PENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE-- 210

Query: 210 FKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDRDHAGANM 269
              + ++G I   P FGG ERTESE  L  +  +T+   DW+W+AFLP GEDR+H   N 
Sbjct: 211 -SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNP 270

Query: 270 NGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNAFHGFFGF 329
             P G+ + G+ +FP +++   GLDL+ D Q +Y E LK+ G++VKL+    A  GF+  
Sbjct: 271 FSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLL 330

Query: 330 PDLPEYSLMIEEMSDFI 336
           P+   +  +++E+S F+
Sbjct: 331 PNNNHFHNVMDEISAFV 341

BLAST of CSPI05G30470 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.5e-53
Identity = 118/324 (36.42%), Postives = 182/324 (56.17%), Query Frame = 0

Query: 28  LLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTP----- 87
           L +    R D + NR L  FLD K+P N      V S D  +D++ +L  RI+ P     
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  ---NPTAALDESL---PLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISVNY 147
              + T  L + L    ++P++ +FHGG F   SA++   D   R        VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPEFRFPCQYDDGFDALKFID-----EMDDDSLLERVDLSRCFILGESAGGNLGHHVA 207
           R +PE R+PC YDDG++AL ++      +   DS +        ++ G+S+GGN+ H+VA
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNV------YVYLAGDSSGGNIAHNVA 208

Query: 208 VRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDR 267
           VRA+    + VK++G I   P FGG+ERT+SE  L  +  +T+   DW+WRA+LP GEDR
Sbjct: 209 VRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDR 268

Query: 268 DHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNA 327
           DH   N  GP G+ + G+ NFP +++   GLDL+ D Q +Y + LK+ G +V L+    A
Sbjct: 269 DHPACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQA 328

Query: 328 FHGFFGFPDLPEYSLMIEEMSDFI 336
             GF+  P+   +  ++EE++ F+
Sbjct: 329 TIGFYFLPNNDHFHCLMEELNKFV 341

BLAST of CSPI05G30470 vs. ExPASy TrEMBL
Match: A0A0A0KXS8 (Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 9.2e-195
Identity = 336/339 (99.12%), Postives = 337/339 (99.41%), Query Frame = 0

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M SSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
           SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180
           RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 241 WRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300
           WRAFLPAGEDRDHA AN+NGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM 340
           KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM 339

BLAST of CSPI05G30470 vs. ExPASy TrEMBL
Match: A0A1S3CBA4 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 3.0e-177
Identity = 305/337 (90.50%), Postives = 319/337 (94.66%), Query Frame = 0

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M SS VCY++STPQLPWKH++TLRFATLL+NTSLRSD TVNRRLL F+DPKIP N +S H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
           SVSSSDL +DTSRDLFLRIFTP PTAALDESLPLLPIIFYFHGGGF FGSADATSTDMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180
           R FA+KLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLS CFILGE
Sbjct: 121 RRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSCCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTL MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300
           WRAFLPAGEDRDHA AN+NGPNGRDISGLENFPAT+IFAGGLDLL+DRQK YYERLKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAK 338
           KDVKLVVFSNA HG+FGF DLPEYSLMI+EM DFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of CSPI05G30470 vs. ExPASy TrEMBL
Match: A0A5D3BL91 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001330 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.1e-175
Identity = 303/337 (89.91%), Postives = 317/337 (94.07%), Query Frame = 0

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M SS VCY++STPQLPWKH++TLRFATLL+NTSLRSD TVNRRLL F+DPKIP N +S H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
           SVSSSDL +DTSRDLFLRIFTP PTAALDESLPLLPIIFYFHGGGF FGSADATSTDMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180
           R FA+KL AVVISVNYRLAPEFRFPCQ DDGFD LKFIDEMDDDSLLERVDLSRCFILGE
Sbjct: 121 RRFAKKLCAVVISVNYRLAPEFRFPCQCDDGFDPLKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTL MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300
           WRAFLPAGEDRDHA AN+NGPNGRDISGLENFPAT+IFAGGLDLL+DRQK YYERLKRMG
Sbjct: 241 WRAFLPAGEDRDHATANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAK 338
           KDVKLVVFSNA HG+FGF DLPEYSLMI+EM DFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of CSPI05G30470 vs. ExPASy TrEMBL
Match: A0A6J1ECC8 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.3e-153
Identity = 270/337 (80.12%), Postives = 294/337 (87.24%), Query Frame = 0

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M  SSV  E+ST QLPWKH++TLRFA LLFN S+RSD TVNRRLL FLDPKIPPNPNS H
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
           +VSSSDLTID SRDLFLRIFTP   AA D  LP  P+IFYFHGGGFAFGSADATS DMAA
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPT-VAATDSVLP--PVIFYFHGGGFAFGSADATSMDMAA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDS-LLERVDLSRCFILG 180
           R F+++L AVVISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDDD  LL + DLSRCFILG
Sbjct: 121 RRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILG 180

Query: 181 ESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDW 240
           ESAGGNLGHHVAVRASEY FKRVK+IGFIASQPFFGG+ERTESE RLC Q PLTL + DW
Sbjct: 181 ESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADW 240

Query: 241 FWRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRM 300
           FW+AFLPAGEDRDHA AN+NGPNGRD+SGL NFPAT+I AGGLDLL+DRQ+ YYE LKRM
Sbjct: 241 FWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRM 300

Query: 301 GKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIA 337
           GK+VKLVVF+N  HGFF F D PEYSLMI+EM DFIA
Sbjct: 301 GKEVKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIA 334

BLAST of CSPI05G30470 vs. ExPASy TrEMBL
Match: A0A6J1IER7 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 7.4e-152
Identity = 267/332 (80.42%), Postives = 290/332 (87.35%), Query Frame = 0

Query: 5   SVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSS 64
           SV  E+ST QLPWKH++TLRFA LLFN S+RSD TVNRRLL FLDPKIPPNPNS H+VSS
Sbjct: 5   SVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSS 64

Query: 65  SDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAARGFA 124
           SDLTID SRDLFLRIFTP   AA D  LP  P+IFYFHGGGFAFGSA ATS DMAAR F+
Sbjct: 65  SDLTIDASRDLFLRIFTPT-VAATDSLLP--PVIFYFHGGGFAFGSAAATSMDMAARRFS 124

Query: 125 EKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD-DSLLERVDLSRCFILGESAG 184
           ++L AVVISVNYRL+P+FRFPCQYDDGFDA+KFIDEMDD D LL R DLSRCFILGESAG
Sbjct: 125 KELGAVVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSRCFILGESAG 184

Query: 185 GNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRA 244
           GNLGHHVAVRASEY FKRVK+IGFIASQPFFGG+ERTESE RLC Q PLTL + DWFW+A
Sbjct: 185 GNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKA 244

Query: 245 FLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDV 304
           FLPAGEDRDHA AN+NGPNGRD+SGL NFPAT+I AGGLD L+DRQ+ YYE LKRMGKDV
Sbjct: 245 FLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYEELKRMGKDV 304

Query: 305 KLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFI 336
           KLVVF+N  HGFF F DLPEYSLMI+EM DFI
Sbjct: 305 KLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI 333

BLAST of CSPI05G30470 vs. NCBI nr
Match: XP_004141618.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein Csa_008680 [Cucumis sativus])

HSP 1 Score: 689.1 bits (1777), Expect = 1.9e-194
Identity = 336/339 (99.12%), Postives = 337/339 (99.41%), Query Frame = 0

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M SSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
           SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180
           RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 241 WRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300
           WRAFLPAGEDRDHA AN+NGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM 340
           KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM 339

BLAST of CSPI05G30470 vs. NCBI nr
Match: XP_008459713.1 (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 630.9 bits (1626), Expect = 6.2e-177
Identity = 305/337 (90.50%), Postives = 319/337 (94.66%), Query Frame = 0

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M SS VCY++STPQLPWKH++TLRFATLL+NTSLRSD TVNRRLL F+DPKIP N +S H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
           SVSSSDL +DTSRDLFLRIFTP PTAALDESLPLLPIIFYFHGGGF FGSADATSTDMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180
           R FA+KLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLS CFILGE
Sbjct: 121 RRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSCCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTL MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300
           WRAFLPAGEDRDHA AN+NGPNGRDISGLENFPAT+IFAGGLDLL+DRQK YYERLKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAK 338
           KDVKLVVFSNA HG+FGF DLPEYSLMI+EM DFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of CSPI05G30470 vs. NCBI nr
Match: KAA0039340.1 (putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00523.1 putative carboxylesterase 18 [Cucumis melo var. makuwa])

HSP 1 Score: 624.8 bits (1610), Expect = 4.4e-175
Identity = 303/337 (89.91%), Postives = 317/337 (94.07%), Query Frame = 0

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M SS VCY++STPQLPWKH++TLRFATLL+NTSLRSD TVNRRLL F+DPKIP N +S H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
           SVSSSDL +DTSRDLFLRIFTP PTAALDESLPLLPIIFYFHGGGF FGSADATSTDMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180
           R FA+KL AVVISVNYRLAPEFRFPCQ DDGFD LKFIDEMDDDSLLERVDLSRCFILGE
Sbjct: 121 RRFAKKLCAVVISVNYRLAPEFRFPCQCDDGFDPLKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTL MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300
           WRAFLPAGEDRDHA AN+NGPNGRDISGLENFPAT+IFAGGLDLL+DRQK YYERLKRMG
Sbjct: 241 WRAFLPAGEDRDHATANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAK 338
           KDVKLVVFSNA HG+FGF DLPEYSLMI+EM DFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of CSPI05G30470 vs. NCBI nr
Match: XP_022925491.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 551.6 bits (1420), Expect = 4.8e-153
Identity = 270/337 (80.12%), Postives = 294/337 (87.24%), Query Frame = 0

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M  SSV  E+ST QLPWKH++TLRFA LLFN S+RSD TVNRRLL FLDPKIPPNPNS H
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
           +VSSSDLTID SRDLFLRIFTP   AA D  LP  P+IFYFHGGGFAFGSADATS DMAA
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPT-VAATDSVLP--PVIFYFHGGGFAFGSADATSMDMAA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDS-LLERVDLSRCFILG 180
           R F+++L AVVISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDDD  LL + DLSRCFILG
Sbjct: 121 RRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILG 180

Query: 181 ESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDW 240
           ESAGGNLGHHVAVRASEY FKRVK+IGFIASQPFFGG+ERTESE RLC Q PLTL + DW
Sbjct: 181 ESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADW 240

Query: 241 FWRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRM 300
           FW+AFLPAGEDRDHA AN+NGPNGRD+SGL NFPAT+I AGGLDLL+DRQ+ YYE LKRM
Sbjct: 241 FWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRM 300

Query: 301 GKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIA 337
           GK+VKLVVF+N  HGFF F D PEYSLMI+EM DFIA
Sbjct: 301 GKEVKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIA 334

BLAST of CSPI05G30470 vs. NCBI nr
Match: KAG6592331.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025153.1 putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 547.7 bits (1410), Expect = 6.9e-152
Identity = 267/333 (80.18%), Postives = 292/333 (87.69%), Query Frame = 0

Query: 5   SVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSS 64
           SV  E+ST QLPWKH++TLRFA LLFN S+RSD TVNRRLL FLDPKIPPNPNS H+VSS
Sbjct: 5   SVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSS 64

Query: 65  SDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAARGFA 124
           SDLTID SRDLFLRIFTP   AA D  LP  P+IFYFHGGGFAFGSA ATS DMAAR F+
Sbjct: 65  SDLTIDDSRDLFLRIFTPT-VAATDSVLP--PVIFYFHGGGFAFGSAAATSMDMAARRFS 124

Query: 125 EKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDS-LLERVDLSRCFILGESAG 184
           ++L AVVISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDDD  LL + DLSRCFILGESAG
Sbjct: 125 KELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAG 184

Query: 185 GNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRA 244
           GNLGHHVAVRASEY FKRVK+IGFIASQPFFGG+ERTESE RLC Q PLTL + DWFW+A
Sbjct: 185 GNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKA 244

Query: 245 FLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDV 304
           FLPAGEDRDHA AN+NGPNGRD+SGL NFPAT+I AGGLD+L+DRQ+ YYE LKRMGK+V
Sbjct: 245 FLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEV 304

Query: 305 KLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIA 337
           KLVVF+N  HGFF F DLPEYSLMI+EM DFIA
Sbjct: 305 KLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 334

BLAST of CSPI05G30470 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 295.0 bits (754), Expect = 7.5e-80
Identity = 152/324 (46.91%), Postives = 202/324 (62.35%), Query Frame = 0

Query: 15  LPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRD 74
           LP K ++ L   + + + + R D T+NRR L   D + PPNP   + VS+SD  +D SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISV 134
           L+ R++TP+ +         +P++ +FHGGGFAF S +A   D   R FA KL A VISV
Sbjct: 73  LWFRLYTPHVSG------DKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISV 132

Query: 135 NYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGESAGGNLGHHVAV-- 194
           NYRLAPE R+P QYDDGFDALK+I+E     L    DLSRCF  G+SAGGN+ H+VA+  
Sbjct: 133 NYRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRI 192

Query: 195 -RASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDR 254
            R     F  VK+IG I+ QPFFGG+ERTE+E +L     ++   TDW W+A    G +R
Sbjct: 193 CREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNR 252

Query: 255 DHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNA 314
           DH   N+ GPN  DISGL+ +P T++   G D L D Q+SYYE LK  GK   L+ + N 
Sbjct: 253 DHEAVNVGGPNAVDISGLD-YPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNM 312

Query: 315 FHGFFGFPDLPEYSLMIEEMSDFI 336
           FH F+ FP+LPE   +I  + DF+
Sbjct: 313 FHAFYIFPELPEAGQLIMRIKDFV 326

BLAST of CSPI05G30470 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.6e-61
Identity = 134/343 (39.07%), Postives = 191/343 (55.69%), Query Frame = 0

Query: 2   GSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHS 61
           GS  V    S   +P    V +    L +N   R D T NR L  FLD K+P N N  + 
Sbjct: 3   GSEEVNLIESKTVVPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNG 62

Query: 62  VSSSDLTIDTSRDLFLRIFTP----NPTAALDESLP----LLPIIFYFHGGGFAFGSADA 121
           V S D+ ID   +L  R++ P       +  D   P    ++P+I +FHGG FA  SA++
Sbjct: 63  VFSFDVIIDRQTNLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANS 122

Query: 122 TSTDMAARGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLS 181
              D   R       AVV+SVNYR APE R+PC YDDG+  LK+++      L  + D  
Sbjct: 123 AIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVN--SSSWLRSKKDSK 182

Query: 182 -RCFILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPL 241
            R F+ G+S+GGN+ H+VAVRA E    R+ ++G I   P FGG ERTESE RL  +  +
Sbjct: 183 VRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFV 242

Query: 242 TLYMTDWFWRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSY 301
           T+   DW+WRAFLP GEDR+H   +  GP  + + GL +FP +++   GLDL+ D Q  Y
Sbjct: 243 TVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKY 302

Query: 302 YERLKRMGKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFI 336
            E LK+ G++VKL+    A  GF+  P+   +  +++E++ F+
Sbjct: 303 AEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of CSPI05G30470 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 220.7 bits (561), Expect = 1.8e-57
Identity = 121/317 (38.17%), Postives = 179/317 (56.47%), Query Frame = 0

Query: 30  FNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTP------N 89
           +N   R D T NR L  +LD K+  N N    V S D+ ID   +L  R++ P       
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  PTAALDESLP----LLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISVNYRLA 149
           P + LD   P    ++P+I +FHGG FA  SA++   D   R      + VV+SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLS-RCFILGESAGGNLGHHVAVRASEYE 209
           PE  +PC YDDG+ AL +++      L  + D     F+ G+S+GGN+ H+VA+RA E  
Sbjct: 151 PENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE-- 210

Query: 210 FKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDRDHAGANM 269
              + ++G I   P FGG ERTESE  L  +  +T+   DW+W+AFLP GEDR+H   N 
Sbjct: 211 -SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNP 270

Query: 270 NGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNAFHGFFGF 329
             P G+ + G+ +FP +++   GLDL+ D Q +Y E LK+ G++VKL+    A  GF+  
Sbjct: 271 FSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLL 330

Query: 330 PDLPEYSLMIEEMSDFI 336
           P+   +  +++E+S F+
Sbjct: 331 PNNNHFHNVMDEISAFV 341

BLAST of CSPI05G30470 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 211.5 bits (537), Expect = 1.1e-54
Identity = 118/324 (36.42%), Postives = 182/324 (56.17%), Query Frame = 0

Query: 28  LLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTP----- 87
           L +    R D + NR L  FLD K+P N      V S D  +D++ +L  RI+ P     
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  ---NPTAALDESL---PLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISVNY 147
              + T  L + L    ++P++ +FHGG F   SA++   D   R        VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPEFRFPCQYDDGFDALKFID-----EMDDDSLLERVDLSRCFILGESAGGNLGHHVA 207
           R +PE R+PC YDDG++AL ++      +   DS +        ++ G+S+GGN+ H+VA
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNV------YVYLAGDSSGGNIAHNVA 208

Query: 208 VRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDR 267
           VRA+    + VK++G I   P FGG+ERT+SE  L  +  +T+   DW+WRA+LP GEDR
Sbjct: 209 VRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDR 268

Query: 268 DHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNA 327
           DH   N  GP G+ + G+ NFP +++   GLDL+ D Q +Y + LK+ G +V L+    A
Sbjct: 269 DHPACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQA 328

Query: 328 FHGFFGFPDLPEYSLMIEEMSDFI 336
             GF+  P+   +  ++EE++ F+
Sbjct: 329 TIGFYFLPNNDHFHCLMEELNKFV 341

BLAST of CSPI05G30470 vs. TAIR 10
Match: AT2G45600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 169.1 bits (427), Expect = 6.2e-42
Identity = 105/295 (35.59%), Postives = 157/295 (53.22%), Query Frame = 0

Query: 31  NTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTPNPTAALDE 90
           N +L SD ++ R    F  PK+PP      +  S D+ ++ + + F+RIF P       +
Sbjct: 15  NITLNSDGSLTRH-RDF--PKLPP------TEQSKDIPLNQTNNTFIRIFKPRNIPPESK 74

Query: 91  SLPLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISVNYRLAPEFRFPCQYDD 150
               LPI+ YFHGGGF   SA +     +    A++L+ +++SV YRLAPE R P  Y+D
Sbjct: 75  ----LPILVYFHGGGFILYSAASAPFHESCTKMADRLQTIILSVEYRLAPEHRLPAAYED 134

Query: 151 GFDALKFIDEMD---------DDSLLERVDLSRCFILGESAGGNLGHHVAVRASEYEFKR 210
             +A+ ++ +           D  L + VD S+C+++G S+GGN+ ++VA+R  + +   
Sbjct: 135 AVEAILWLRDQARGPINGGDCDTWLKDGVDFSKCYVMGSSSGGNIVYNVALRVVDTDLSP 194

Query: 211 VKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDRDHAGAN---M 270
           VKI G I +Q FFGG E ++SE+RL       L  T   W   LP G DRDH  +N    
Sbjct: 195 VKIQGLIMNQAFFGGVEPSDSESRLKDDKICPLPATHLLWSLCLPDGVDRDHVYSNPIKS 254

Query: 271 NGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNAFH 314
           +GP  +D  G   FP+T+I   G D L+DRQ+   E LK  G  V+     + FH
Sbjct: 255 SGPQEKDKMG--RFPSTLINGYGGDPLVDRQRHVAEMLKGRGVHVETRFDKDGFH 294

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT101.1e-7846.91Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G62.2e-6039.07Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q6L5455.4e-5941.10Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q9MAA72.5e-5638.17Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Q9LYC11.5e-5336.42Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KXS89.2e-19599.12Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1[more]
A0A1S3CBA43.0e-17790.50probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1[more]
A0A5D3BL912.1e-17589.91Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1ECC82.3e-15380.12probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 ... [more]
A0A6J1IER77.4e-15280.42probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_004141618.11.9e-19499.12probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein ... [more]
XP_008459713.16.2e-17790.50PREDICTED: probable carboxylesterase 18 [Cucumis melo][more]
KAA0039340.14.4e-17589.91putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00523.1 putative car... [more]
XP_022925491.14.8e-15380.12probable carboxylesterase 18 [Cucurbita moschata][more]
KAG6592331.16.9e-15280.18putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
Match NameE-valueIdentityDescription
AT5G23530.17.5e-8046.91carboxyesterase 18 [more]
AT5G27320.11.6e-6139.07alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.11.8e-5738.17alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.11.1e-5436.42alpha/beta-Hydrolases superfamily protein [more]
AT2G45600.16.2e-4235.59alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 98..316
e-value: 9.1E-52
score: 176.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 2..336
e-value: 5.6E-84
score: 284.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 46..337
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 11..336
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 11..336
IPR002168Lipase, GDXG, putative histidine active sitePROSITEPS01173LIPASE_GDXG_HIScoord: 98..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G30470.1CSPI05G30470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity