CSPI05G30430 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G30430
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptioncationic amino acid transporter 1-like
LocationChr5: 28346684 .. 28348964 (+)
RNA-Seq ExpressionCSPI05G30430
SyntenyCSPI05G30430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTGCTTTACCTTAAGAGGGATTCTGATCTCTTTTCTCCACCCTCTTGTTCACTCCTCGCCCACCTCAGGTTAGTTTTTCAACGTATGATTCTCATATAACCATTTCAGACTTACATAAATCTTGTTTTAGTTCTTACTATAAATTAAACCATGTTGTCTCGTATGTTTTAGGGTCAAAGCAAGCTAAGTTCATACACTACTGACTTGAATGTTAGTTGTCTTTGGAGGGTTAAAAAATGAGACTAACTAATAAAACTTTATGAGTTATTTTGAACTTTTTGTTCTTTTTTCAGCGTGAGATAGGAATGGGGCAGCCGAGCAGTGATGGTAGTAATGGAGAAGGGATCAGAAGAAGATCATGTGGTTGTGGGAAAGATGATTTTCTACCAGAGGAGTCCTTTCAGAGTTGGGAAAATTATACAAAAGCACTTAAGGCAACCCCGTTTCGTCTCTTCAACCGGCTCACGGCCCGATCAGCGGATCATACCGAGCTTGTTGAGATGAAGGCGAGAAGCCAGCATCAGATGAAGAAGACACTCACATGGTGGGACCTCATGTGGTTTGGCGTTGGCGCCGTCATTGGTGCTGGTATATTCGTCCTCACCGGTCTCGAAACGAAGAAACATGCTGGCCCTGCAGTCGTCTTGTCTTACATTGTCTCTGGCATCTCTGCCATGCTTTCTGTCTTCTGCTACACTGAGTTTGCCGTTGAGATTCCAGCTGCCGGTATGTGCATGCTTCTTTGCATAAGGTCTCATATATGAATTGTGACTCAAAACTTATTTAACTAGAAAGGTTTTTCCAGGTGGATCATTTGCGTACCTAAGGGTAGAATTAGGGGACTTTGTGGCATTCATCGCTGCCGGAAACATTCTTCTCGAATACATTATCGGAAGTGCAGCTGTAGCTCGTGCATGGACGTCATACTTCGCCACCCTCTGCAACCACCATCCAAACGATTTTCGTGTCCACATATCCGCTTTCCCTGACGACTACAACCAACTCGATCCCATCGCCATTGTCGTCATTCTCATCATCGGCATCTTCGCTCTAGTGAGCACCAAAGGCTCCTCCCGCCTCAACTACGTTGCCTCCATTTTGCACGTTGTTGTCATCGTCTTCATCATCATTGCTGGACTAACTCAAGCCAACCCGAAAAACTTCACTCCCTTTGCCCCTTATGGCCCTCGTGGCATTTTCGTGGCTTCCGCCGTCTTGTTCTTTGCATACGTCGGGTTCGATGCGGTGTCGACATTGGCAGAAGAGACTAAGAATCCAGCTAAAGATATCCCCATAGGACTTGTTGGATCCATGGTGATCACAACCGTTCTATATTGCATTCTTGCTGTTACGCTCTGCCTCATGCAACCGTATCAACAAATTGATGCAGATGCACCGTTTTCTATGGCTTTTGAGGCCGTTGGTTGGAGTTGGGCTAAGTACATTGTCGCTGCTGGTGCTATTAAAGGCATGACAACTGTGTTGTTGGTTGGTTCGGTTGGTCAAGCTCGGTATCTCACACACATTGCTAGAACCCACATGGTGTCTCCATGGTTTGCCAAAGTCCATGAAAAAACTGGTACTGTCATTGCATCCATCCTTGTTTAGTCTAGACGACACACCAATACTAAAATTTGGATTCCATTTAGAGTATTAAATCTTTTTATCTGACATTATATGTTGGCAACTAAATTGAAAATGTTTAATTTTCTAAAACAGGAACTCCTGTAAACGCAACAGCAACAATGCTCATTGCAACAGCGATGGTTGCTTTCTTCACATCAATGAATATCCTCTCAAACCTTCTCTCAATCTCCACCCTCTTCATCTTCATGTTAGTAGCCATAGCCCTTATCATCCGACGCTACTACAGCAGCAGCGAAACCACAACATCAAACCGAAACAAGCTCTTAATATACCTAGTTCTGATAATCGGATCTTCAATGGCAACTGCCGCATATTGGAGCACAAGCGACAATTGGATCGGCTATGCCGTGACCCTTCCAATATGGTTCTTCTCAACTCTAGCTCTATGGCTCGGCGTCCCACAGGCAAAGAAGCCACAAGTCTGGGGTGTGCCATTGGTCCCATGGCTGCCTTCACTGTCCATTGCCATTAACTTTTTCCTTCTTGGGTCCATTGATAGAGCTTCATTTGAAAGGTTTGGGATATGGACTGGGATTCTCTTGATTTACTATTTCTTATTTGGATTGCATGCTTCTTATGACACTGCCATGAACTCCAAGACCAAAACAGCAGAAATTAATGACAGCGTGTGA

mRNA sequence

CTTTGCTTTACCTTAAGAGGGATTCTGATCTCTTTTCTCCACCCTCTTGTTCACTCCTCGCCCACCTCAGCGTGAGATAGGAATGGGGCAGCCGAGCAGTGATGGTAGTAATGGAGAAGGGATCAGAAGAAGATCATGTGGTTGTGGGAAAGATGATTTTCTACCAGAGGAGTCCTTTCAGAGTTGGGAAAATTATACAAAAGCACTTAAGGCAACCCCGTTTCGTCTCTTCAACCGGCTCACGGCCCGATCAGCGGATCATACCGAGCTTGTTGAGATGAAGGCGAGAAGCCAGCATCAGATGAAGAAGACACTCACATGGTGGGACCTCATGTGGTTTGGCGTTGGCGCCGTCATTGGTGCTGGTATATTCGTCCTCACCGGTCTCGAAACGAAGAAACATGCTGGCCCTGCAGTCGTCTTGTCTTACATTGTCTCTGGCATCTCTGCCATGCTTTCTGTCTTCTGCTACACTGAGTTTGCCGTTGAGATTCCAGCTGCCGGTGGATCATTTGCGTACCTAAGGGTAGAATTAGGGGACTTTGTGGCATTCATCGCTGCCGGAAACATTCTTCTCGAATACATTATCGGAAGTGCAGCTGTAGCTCGTGCATGGACGTCATACTTCGCCACCCTCTGCAACCACCATCCAAACGATTTTCGTGTCCACATATCCGCTTTCCCTGACGACTACAACCAACTCGATCCCATCGCCATTGTCGTCATTCTCATCATCGGCATCTTCGCTCTAGTGAGCACCAAAGGCTCCTCCCGCCTCAACTACGTTGCCTCCATTTTGCACGTTGTTGTCATCGTCTTCATCATCATTGCTGGACTAACTCAAGCCAACCCGAAAAACTTCACTCCCTTTGCCCCTTATGGCCCTCGTGGCATTTTCGTGGCTTCCGCCGTCTTGTTCTTTGCATACGTCGGGTTCGATGCGGTGTCGACATTGGCAGAAGAGACTAAGAATCCAGCTAAAGATATCCCCATAGGACTTGTTGGATCCATGGTGATCACAACCGTTCTATATTGCATTCTTGCTGTTACGCTCTGCCTCATGCAACCGTATCAACAAATTGATGCAGATGCACCGTTTTCTATGGCTTTTGAGGCCGTTGGTTGGAGTTGGGCTAAGTACATTGTCGCTGCTGGTGCTATTAAAGGCATGACAACTGTGTTGTTGGTTGGTTCGGTTGGTCAAGCTCGGTATCTCACACACATTGCTAGAACCCACATGGTGTCTCCATGGTTTGCCAAAGTCCATGAAAAAACTGGAACTCCTGTAAACGCAACAGCAACAATGCTCATTGCAACAGCGATGGTTGCTTTCTTCACATCAATGAATATCCTCTCAAACCTTCTCTCAATCTCCACCCTCTTCATCTTCATGTTAGTAGCCATAGCCCTTATCATCCGACGCTACTACAGCAGCAGCGAAACCACAACATCAAACCGAAACAAGCTCTTAATATACCTAGTTCTGATAATCGGATCTTCAATGGCAACTGCCGCATATTGGAGCACAAGCGACAATTGGATCGGCTATGCCGTGACCCTTCCAATATGGTTCTTCTCAACTCTAGCTCTATGGCTCGGCGTCCCACAGGCAAAGAAGCCACAAGTCTGGGGTGTGCCATTGGTCCCATGGCTGCCTTCACTGTCCATTGCCATTAACTTTTTCCTTCTTGGGTCCATTGATAGAGCTTCATTTGAAAGGTTTGGGATATGGACTGGGATTCTCTTGATTTACTATTTCTTATTTGGATTGCATGCTTCTTATGACACTGCCATGAACTCCAAGACCAAAACAGCAGAAATTAATGACAGCGTGTGA

Coding sequence (CDS)

ATGGGGCAGCCGAGCAGTGATGGTAGTAATGGAGAAGGGATCAGAAGAAGATCATGTGGTTGTGGGAAAGATGATTTTCTACCAGAGGAGTCCTTTCAGAGTTGGGAAAATTATACAAAAGCACTTAAGGCAACCCCGTTTCGTCTCTTCAACCGGCTCACGGCCCGATCAGCGGATCATACCGAGCTTGTTGAGATGAAGGCGAGAAGCCAGCATCAGATGAAGAAGACACTCACATGGTGGGACCTCATGTGGTTTGGCGTTGGCGCCGTCATTGGTGCTGGTATATTCGTCCTCACCGGTCTCGAAACGAAGAAACATGCTGGCCCTGCAGTCGTCTTGTCTTACATTGTCTCTGGCATCTCTGCCATGCTTTCTGTCTTCTGCTACACTGAGTTTGCCGTTGAGATTCCAGCTGCCGGTGGATCATTTGCGTACCTAAGGGTAGAATTAGGGGACTTTGTGGCATTCATCGCTGCCGGAAACATTCTTCTCGAATACATTATCGGAAGTGCAGCTGTAGCTCGTGCATGGACGTCATACTTCGCCACCCTCTGCAACCACCATCCAAACGATTTTCGTGTCCACATATCCGCTTTCCCTGACGACTACAACCAACTCGATCCCATCGCCATTGTCGTCATTCTCATCATCGGCATCTTCGCTCTAGTGAGCACCAAAGGCTCCTCCCGCCTCAACTACGTTGCCTCCATTTTGCACGTTGTTGTCATCGTCTTCATCATCATTGCTGGACTAACTCAAGCCAACCCGAAAAACTTCACTCCCTTTGCCCCTTATGGCCCTCGTGGCATTTTCGTGGCTTCCGCCGTCTTGTTCTTTGCATACGTCGGGTTCGATGCGGTGTCGACATTGGCAGAAGAGACTAAGAATCCAGCTAAAGATATCCCCATAGGACTTGTTGGATCCATGGTGATCACAACCGTTCTATATTGCATTCTTGCTGTTACGCTCTGCCTCATGCAACCGTATCAACAAATTGATGCAGATGCACCGTTTTCTATGGCTTTTGAGGCCGTTGGTTGGAGTTGGGCTAAGTACATTGTCGCTGCTGGTGCTATTAAAGGCATGACAACTGTGTTGTTGGTTGGTTCGGTTGGTCAAGCTCGGTATCTCACACACATTGCTAGAACCCACATGGTGTCTCCATGGTTTGCCAAAGTCCATGAAAAAACTGGAACTCCTGTAAACGCAACAGCAACAATGCTCATTGCAACAGCGATGGTTGCTTTCTTCACATCAATGAATATCCTCTCAAACCTTCTCTCAATCTCCACCCTCTTCATCTTCATGTTAGTAGCCATAGCCCTTATCATCCGACGCTACTACAGCAGCAGCGAAACCACAACATCAAACCGAAACAAGCTCTTAATATACCTAGTTCTGATAATCGGATCTTCAATGGCAACTGCCGCATATTGGAGCACAAGCGACAATTGGATCGGCTATGCCGTGACCCTTCCAATATGGTTCTTCTCAACTCTAGCTCTATGGCTCGGCGTCCCACAGGCAAAGAAGCCACAAGTCTGGGGTGTGCCATTGGTCCCATGGCTGCCTTCACTGTCCATTGCCATTAACTTTTTCCTTCTTGGGTCCATTGATAGAGCTTCATTTGAAAGGTTTGGGATATGGACTGGGATTCTCTTGATTTACTATTTCTTATTTGGATTGCATGCTTCTTATGACACTGCCATGAACTCCAAGACCAAAACAGCAGAAATTAATGACAGCGTGTGA

Protein sequence

MGQPSSDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADHTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSGISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTSYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILHVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAKDIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAIKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTSMNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYWSTSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSIDRASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV*
Homology
BLAST of CSPI05G30430 vs. ExPASy Swiss-Prot
Match: Q84MA5 (Cationic amino acid transporter 1 OS=Arabidopsis thaliana OX=3702 GN=CAT1 PE=1 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 1.9e-237
Identity = 401/566 (70.85%), Postives = 478/566 (84.45%), Query Frame = 0

Query: 6   SDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADHTELVE 65
           + G   +G+RRR C C KDDFLPEESFQS  NY KALK TP R  +R+  RS D  E+ E
Sbjct: 2   ASGGGDDGLRRRGCSCTKDDFLPEESFQSMGNYLKALKETPSRFMDRIMTRSLDSDEINE 61

Query: 66  MKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSGISAML 125
           MKARS H+MKKTLTWWDLMWFG+GAVIG+GIFVLTGLE + H+GPAVVLSY+VSG+SAML
Sbjct: 62  MKARSGHEMKKTLTWWDLMWFGIGAVIGSGIFVLTGLEARNHSGPAVVLSYVVSGVSAML 121

Query: 126 SVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTSYFATL 185
           SVFCYTEFAVEIP AGGSFAYLRVELGDF+AFIAAGNI+LEY++G AAVAR+WTSYFATL
Sbjct: 122 SVFCYTEFAVEIPVAGGSFAYLRVELGDFMAFIAAGNIILEYVVGGAAVARSWTSYFATL 181

Query: 186 CNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILHVVVIV 245
            NH P DFR+ +    +DY+ LDPIA+ V  II + A+V TKGSSR NY+ASI+H+VVI+
Sbjct: 182 LNHKPEDFRIIVHKLGEDYSHLDPIAVGVCAIICVLAVVGTKGSSRFNYIASIIHMVVIL 241

Query: 246 FIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAKDIPIG 305
           F+IIAG T+A+ KN++ F PYG RG+F ++AVLFFAY+GFDAVST+AEETKNP +DIPIG
Sbjct: 242 FVIIAGFTKADVKNYSDFTPYGVRGVFKSAAVLFFAYIGFDAVSTMAEETKNPGRDIPIG 301

Query: 306 LVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAIKGMTT 365
           LVGSMV+TTV YC++AVTLCLMQPYQQID DAPFS+AF AVGW WAKYIVA GA+KGMTT
Sbjct: 302 LVGSMVVTTVCYCLMAVTLCLMQPYQQIDPDAPFSVAFSAVGWDWAKYIVAFGALKGMTT 361

Query: 366 VLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTSMNILS 425
           VLLVG++GQARY+THIAR HM+ PW A+V+ KTGTP+NAT  ML ATA++AFFT + IL+
Sbjct: 362 VLLVGAIGQARYMTHIARAHMMPPWLAQVNAKTGTPINATVVMLAATALIAFFTKLKILA 421

Query: 426 NLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYWS-TSD 485
           +LLS+STLFIFM VA+AL++RRYY + ET+T +RNK L++L LI+ SS ATA YW+   +
Sbjct: 422 DLLSVSTLFIFMFVAVALLVRRYYVTGETSTRDRNKFLVFLGLILASSTATAVYWALEEE 481

Query: 486 NWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSIDRASF 545
            WIGY +T+PIWF ST+A+   VPQA+ P++WGVPLVPWLPS SIAIN FLLGSID  SF
Sbjct: 482 GWIGYCITVPIWFLSTVAMKFLVPQARAPKIWGVPLVPWLPSASIAINIFLLGSIDTKSF 541

Query: 546 ERFGIWTGILLIYYFLFGLHASYDTA 571
            RF IWTGILLIYY LFGLHA+YDTA
Sbjct: 542 VRFAIWTGILLIYYVLFGLHATYDTA 567

BLAST of CSPI05G30430 vs. ExPASy Swiss-Prot
Match: O64759 (Cationic amino acid transporter 5 OS=Arabidopsis thaliana OX=3702 GN=CAT5 PE=1 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 1.5e-186
Identity = 330/561 (58.82%), Postives = 425/561 (75.76%), Query Frame = 0

Query: 12  EGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADHTELVEMKARSQ 71
           EG  R      K DF PEESFQS+ +Y  AL  T  R  NRL +RS D  E  E+K +S+
Sbjct: 2   EGEERGYWRWSKRDFFPEESFQSFGSYRAALSQTCSRFKNRLVSRSDDENERFELKKQSE 61

Query: 72  HQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSGISAMLSVFCYT 131
           H+MK+ LTWWDL+WFG G+VIGAGIFVLTG E  + AGPA+VLSY+VSG+SAMLSVFCYT
Sbjct: 62  HEMKRCLTWWDLVWFGFGSVIGAGIFVLTGQEAHEQAGPAIVLSYVVSGLSAMLSVFCYT 121

Query: 132 EFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTSYFATLCNHHPN 191
           EFAVEIP AGGSFAYLR+ELGDF AFI AGNILLE I+G+AAVARAWTSYFATL N  PN
Sbjct: 122 EFAVEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFATLLNRSPN 181

Query: 192 DFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILHVVVIVFIIIAG 251
             R+  +     +N LDPIA+VVI      A +ST+ +S LN++AS ++ +VI F+IIAG
Sbjct: 182 ALRIK-TDLSSGFNLLDPIAVVVIAASATIASISTRKTSLLNWIASAINTLVIFFVIIAG 241

Query: 252 LTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAKDIPIGLVGSMV 311
              A+  N TPF P+GP G+F A+AV++FAY GFD+++T+AEETKNP++DIPIGL+GSM 
Sbjct: 242 FIHADTSNLTPFLPFGPEGVFRAAAVVYFAYGGFDSIATMAEETKNPSRDIPIGLLGSMS 301

Query: 312 ITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAIKGMTTVLLVGS 371
           I TV+YC++A++L +MQ Y  ID +A +S+AF++VG  W KY+VA GA+KGMTTVLLVG+
Sbjct: 302 IITVIYCLMALSLSMMQKYTDIDPNAAYSVAFQSVGMKWGKYLVALGALKGMTTVLLVGA 361

Query: 372 VGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTSMNILSNLLSIS 431
           +GQARY+THIARTHM+ P FA VH KTGTP+NA   + I +A++AFF+ +++L++LLSIS
Sbjct: 362 LGQARYVTHIARTHMIPPIFALVHPKTGTPINANLLVAIPSALIAFFSGLDVLASLLSIS 421

Query: 432 TLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYW--STSDNWIGY 491
           TLFIF ++ IAL++RRYY   +T   +  KL+  L+ ++ SSM T+AYW      +WIGY
Sbjct: 422 TLFIFTMMPIALLVRRYYVRQDTPRVHLIKLITCLLFVVVSSMGTSAYWGMQRKGSWIGY 481

Query: 492 AVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSIDRASFERFGI 551
            VT+P WF  TL +   VPQ + P+VWGVPLVPWLP LSIA N FL+GS+   +F RFG+
Sbjct: 482 TVTVPFWFLGTLGIVFFVPQQRTPKVWGVPLVPWLPCLSIATNIFLMGSLGAMAFVRFGV 541

Query: 552 WTGILLIYYFLFGLHASYDTA 571
            T  +L+YYFL GLHA++D A
Sbjct: 542 CTLAMLLYYFLLGLHATFDMA 561

BLAST of CSPI05G30430 vs. ExPASy Swiss-Prot
Match: Q9SHH0 (Cationic amino acid transporter 8, vacuolar OS=Arabidopsis thaliana OX=3702 GN=CAT8 PE=2 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 1.8e-163
Identity = 289/549 (52.64%), Postives = 399/549 (72.68%), Query Frame = 0

Query: 23  KDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADHTELVEMKARSQHQMKKTLTWWD 82
           K DF PE SFQS+  Y  AL AT  RL +RL +RS+D  EL   +  S++ M++ LTWWD
Sbjct: 30  KQDFFPEFSFQSFSTYKSALSATCPRLADRLLSRSSDAYELDAARRESENPMRRCLTWWD 89

Query: 83  LMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSGISAMLSVFCYTEFAVEIPAAGG 142
           L+W   G+V+G+G+FV+TG E +  AGPAVVLSY +SG+SA+LSV CY EF VEIP AGG
Sbjct: 90  LLWLSFGSVVGSGVFVITGQEARVGAGPAVVLSYAISGVSALLSVLCYAEFGVEIPVAGG 149

Query: 143 SFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTSYFATLCNHHPNDFRVHISAFPD 202
           SF+YLRVELGDF+AFIAAGNILLE ++G+A + R+W+SY A+L  +  + FR+ + +F  
Sbjct: 150 SFSYLRVELGDFIAFIAAGNILLEAMVGAAGLGRSWSSYLASLVKNDSDYFRIKVDSFAK 209

Query: 203 DYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILHVVVIVFIIIAGLTQANPKNFTP 262
            ++ LDP+A+ V+L+    A+  TK +S LN + S++ V +IVFI++ G T +   N  P
Sbjct: 210 GFDLLDPVAVAVLLVANGIAMTGTKRTSWLNLITSMVTVCIIVFIVVVGFTHSKTSNLVP 269

Query: 263 FAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAKDIPIGLVGSMVITTVLYCILAV 322
           F PYG +G+  ++AV++++Y GFD V+ +AEET+ P++DIPIGLVGSM + TV+YC++A+
Sbjct: 270 FFPYGAKGVVQSAAVVYWSYTGFDMVANMAEETEKPSRDIPIGLVGSMSMITVVYCLMAL 329

Query: 323 TLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAIKGMTTVLLVGSVGQARYLTHIA 382
            L +M  Y +IDA+A +S+AF  +G  WAKY+V   A+KGMTT LLVGS+GQARY T IA
Sbjct: 330 ALTMMVKYTEIDANAAYSVAFAQIGMKWAKYLVGICALKGMTTSLLVGSLGQARYTTQIA 389

Query: 383 RTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTSMNILSNLLSISTLFIFMLVAIA 442
           R+HM+ PWFA VH KTGTP+ AT  + I +++++FFTS+ +LS++ S +TLFIFMLVA+A
Sbjct: 390 RSHMIPPWFALVHPKTGTPIYATLLVTILSSIISFFTSLEVLSSVFSFATLFIFMLVAVA 449

Query: 443 LIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYWSTS-DNWIGYAVTLPIWFFSTL 502
           L++RRYY    T  +   K L +L LII SS+  +A W++    WI Y VT  IWF  TL
Sbjct: 450 LLVRRYYVKDVTPEAGLLKFLGFLFLIIASSIGVSALWNSGVKGWIAYTVTGVIWFIGTL 509

Query: 503 ALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSIDRASFERFGIWTGILLIYYFLF 562
            L L +P+ + P+VWGVPLVPWLPS SIA+N FL+GS+   +F RF I T ++L+YY   
Sbjct: 510 GLAL-LPKYRVPKVWGVPLVPWLPSFSIAMNLFLIGSLGYVAFLRFIICTMVMLLYYLFV 569

Query: 563 GLHASYDTA 571
           GLHA+YD A
Sbjct: 570 GLHATYDVA 577

BLAST of CSPI05G30430 vs. ExPASy Swiss-Prot
Match: Q9LZ20 (Cationic amino acid transporter 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAT6 PE=2 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 3.4e-125
Identity = 234/556 (42.09%), Postives = 355/556 (63.85%), Query Frame = 0

Query: 31  SFQSWENYTKALKATPFRLFNRLTARSADHTELVEMKARSQHQMKKTLTWWDLMWFGVGA 90
           SF S   Y  +L ATP RL  R  + S    E+  ++A S  QM++TL W+DL+  G+G 
Sbjct: 14  SFSSLRVYLNSLSATPSRLSRRAISVSTSSDEMSRVRAVSGEQMRRTLRWYDLIGLGIGG 73

Query: 91  VIGAGIFVLTGLETKKHAGPAVVLSYIVSGISAMLSVFCYTEFAVEIPAAGGSFAYLRVE 150
           ++GAG+FV TG  ++  AGP++V+SY ++G+ A+LS FCYTEFAV +P AGG+F+Y+R+ 
Sbjct: 74  MVGAGVFVTTGRASRLDAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRIT 133

Query: 151 LGDFVAFIAAGNILLEYIIGSAAVARAWTSYFATLCNHHPNDFRVHISAFPDDYNQLDPI 210
            G+F AF    N++++Y++ +AAV+R++T+Y  T      + +R  +S  P  +N++DP+
Sbjct: 134 FGEFPAFFTGANLVMDYVMSNAAVSRSFTAYLGTAFGISTSKWRFVVSGLPKGFNEIDPV 193

Query: 211 AIVVILIIGIFALVSTKGSSRLNYVASILHVVVIVFIIIAGL---------TQANPKNFT 270
           A++V+L+I +    ST+ SS++N + +  H+  I F+I+ G          + ANP++ +
Sbjct: 194 AVLVVLVITVIICCSTRESSKVNMIMTAFHIAFIFFVIVMGFIKGDSKNLSSPANPEHPS 253

Query: 271 PFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAKDIPIGLVGSMVITTVLYCILA 330
            F P+G  G+F  +A+++ +Y+G+DAVST+AEE +NP KDIP+G+ GS+ I TVLYC++A
Sbjct: 254 GFFPFGAAGVFNGAAMVYLSYIGYDAVSTMAEEVENPVKDIPVGVSGSVAIVTVLYCLMA 313

Query: 331 VTLCLMQPYQQIDADAPFSMAFE-AVGWSWAKYIVAAGAIKGMTTVLLVGSVGQARYLTH 390
           V++ ++ PY  ID +APFS AF  + GW W   +V  GA  G+ T LLV  +GQARY+  
Sbjct: 314 VSMSMLLPYDLIDPEAPFSAAFRGSNGWEWVTKVVGIGASFGILTSLLVAMLGQARYMCV 373

Query: 391 IARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTSMNILSNLLSISTLFIFMLVA 450
           I R+ +V  WFAK+H KT TPVNA+  + I TA +A FT +N+L NL+SI TLF+F +VA
Sbjct: 374 IGRSRVVPFWFAKIHPKTSTPVNASTFLGIFTAALALFTDLNVLLNLVSIGTLFVFYMVA 433

Query: 451 IALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYWSTSDNWIGYAVTLPIWFFST 510
            ALI RRY     T        L +L L   +S+     W         A  L       
Sbjct: 434 NALIFRRYVPVGPTKPW---PTLCFLTLFSITSLVFTLIWKLVPEGKPKAFMLGASAVVA 493

Query: 511 LALWLG----VPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSIDRASFERFGIWTGILLI 570
           +A+ L     VPQA+KP++WGVP +PW P +SI +N FLLGS+D  S+ RFG ++G++++
Sbjct: 494 IAIVLSFQCVVPQARKPELWGVPFMPWTPCVSIFLNIFLLGSLDAPSYVRFGFFSGLIVL 553

Query: 571 YYFLFGLHASYDTAMN 573
            Y  +G+HAS D   N
Sbjct: 554 VYLFYGVHASSDAEAN 566

BLAST of CSPI05G30430 vs. ExPASy Swiss-Prot
Match: Q9SQZ0 (Cationic amino acid transporter 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAT7 PE=3 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 2.9e-124
Identity = 231/554 (41.70%), Postives = 357/554 (64.44%), Query Frame = 0

Query: 29  EESFQSWENYTKALKATPFRLFNRLTARSADHTELVEMKARSQHQMKKTLTWWDLMWFGV 88
           + SF S   Y  +L  TP R   R  + S  + E+  ++A S  QM++TL W+DL+  G+
Sbjct: 11  DTSFSSLRVYLNSLSDTPSRFSRRAVSVSTSYDEMSRVRAVSGEQMRRTLRWYDLIGLGI 70

Query: 89  GAVIGAGIFVLTGLETKKHAGPAVVLSYIVSGISAMLSVFCYTEFAVEIPAAGGSFAYLR 148
           G +IGAG+FV TG  ++ +AGP++V+SY ++G+ A+LS FCYTEFAV +P AGG+F+Y+R
Sbjct: 71  GGMIGAGVFVTTGRASRLYAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIR 130

Query: 149 VELGDFVAFIAAGNILLEYIIGSAAVARAWTSYFATLCNHHPNDFRVHISAFPDDYNQLD 208
           +  G+F AFI   N++++Y++ +AAV+R +T+Y  +      +++R  +S  P+ +N++D
Sbjct: 131 ITFGEFPAFITGANLIMDYVLSNAAVSRGFTAYLGSAFGISTSEWRFIVSGLPNGFNEID 190

Query: 209 PIAIVVILIIGIFALVSTKGSSRLNYVASILHVVVIVFIIIAGLTQA---------NPKN 268
           PIA++V+L +      ST+ SS++N V + LH+  IVF+I+ G ++          NP+N
Sbjct: 191 PIAVIVVLAVTFVICYSTRESSKVNMVLTALHIAFIVFVIVMGFSKGDVKNLTRPDNPEN 250

Query: 269 FTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAKDIPIGLVGSMVITTVLYCI 328
            + F P+G  G+F  +A+++ +Y+G+DAVST+AEE K+P KDIP+G+ GS+ I  VLYC+
Sbjct: 251 PSGFFPFGVSGVFNGAAMVYLSYIGYDAVSTMAEEVKDPVKDIPMGISGSVAIVIVLYCL 310

Query: 329 LAVTLCLMQPYQQIDADAPFSMAF-EAVGWSWAKYIVAAGAIKGMTTVLLVGSVGQARYL 388
           +A+++ ++ PY  IDA+AP+S AF ++ GW W   +V  GA  G+ T L+V  +GQARY+
Sbjct: 311 MAISMSMLLPYDLIDAEAPYSAAFSKSEGWEWVTRVVGIGASFGILTSLIVAMLGQARYM 370

Query: 389 THIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTSMNILSNLLSISTLFIFML 448
             I R+ +V  WFAKVH KT TPVNA+A + I TA++A FT +N+L NL+SI TLF+F +
Sbjct: 371 CVIGRSRVVPIWFAKVHPKTSTPVNASAFLGIFTAVLALFTDLNVLLNLVSIGTLFVFYM 430

Query: 449 VAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYW----STSDNWIGYAVTLP 508
           VA A+I RRY +   T        L +L L   +S+     W    S    W     +  
Sbjct: 431 VANAVIFRRYVTVGYTEPW---PTLSFLCLFSITSILFTLVWQLAPSGPPKWFILGASTV 490

Query: 509 IWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSIDRASFERFGIWTGIL 568
                       VPQA+ P+ WGVPL+PW P +SI +N FLLGS+D  S+ RFG ++G++
Sbjct: 491 TAIAIVQIFHCVVPQARIPEFWGVPLMPWTPCVSIFLNIFLLGSLDAPSYIRFGFFSGLV 550

BLAST of CSPI05G30430 vs. ExPASy TrEMBL
Match: A0A0A0KW03 (AA_permease_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647350 PE=4 SV=1)

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 582/584 (99.66%), Postives = 582/584 (99.66%), Query Frame = 0

Query: 1   MGQPSSDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADH 60
           MGQPSSDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADH
Sbjct: 1   MGQPSSDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADH 60

Query: 61  TELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSG 120
           TELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSG
Sbjct: 61  TELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSG 120

Query: 121 ISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTS 180
           ISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTS
Sbjct: 121 ISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTS 180

Query: 181 YFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILH 240
           YFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILH
Sbjct: 181 YFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILH 240

Query: 241 VVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAK 300
           VVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAK
Sbjct: 241 VVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAK 300

Query: 301 DIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAI 360
           DIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAI
Sbjct: 301 DIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAI 360

Query: 361 KGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTS 420
           KGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTS
Sbjct: 361 KGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTS 420

Query: 421 MNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYW 480
           MNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLI LVLIIGSSMATA YW
Sbjct: 421 MNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLICLVLIIGSSMATATYW 480

Query: 481 STSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSID 540
           STSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSID
Sbjct: 481 STSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSID 540

Query: 541 RASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV 585
           RASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV
Sbjct: 541 RASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV 584

BLAST of CSPI05G30430 vs. ExPASy TrEMBL
Match: A0A1S3CBK9 (cationic amino acid transporter 1-like OS=Cucumis melo OX=3656 GN=LOC103498634 PE=4 SV=1)

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 565/585 (96.58%), Postives = 571/585 (97.61%), Query Frame = 0

Query: 1   MGQPSSDGSNG-EGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSAD 60
           MGQPSSDGSNG EGIRRR CGCGKDDFLPEESFQSWENYTKALKATP RLF+RLTARSAD
Sbjct: 1   MGQPSSDGSNGSEGIRRRFCGCGKDDFLPEESFQSWENYTKALKATPLRLFDRLTARSAD 60

Query: 61  HTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVS 120
           HTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVS
Sbjct: 61  HTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVS 120

Query: 121 GISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWT 180
           GISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWT
Sbjct: 121 GISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWT 180

Query: 181 SYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASIL 240
           SYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIV+ILIIG FALVSTKGSSRLNYVASIL
Sbjct: 181 SYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVIILIIGTFALVSTKGSSRLNYVASIL 240

Query: 241 HVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPA 300
           HVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIF ASAVLFFAYVGFDAVSTLAEETKNPA
Sbjct: 241 HVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFEASAVLFFAYVGFDAVSTLAEETKNPA 300

Query: 301 KDIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGA 360
           KDIPIGLVGSMVITT LYCILAVTLCLMQPYQ+IDADAPFSMAFEAVGWSWAKYIVAAGA
Sbjct: 301 KDIPIGLVGSMVITTFLYCILAVTLCLMQPYQKIDADAPFSMAFEAVGWSWAKYIVAAGA 360

Query: 361 IKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFT 420
           IKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATA ML ATA VAFFT
Sbjct: 361 IKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATAAMLAATATVAFFT 420

Query: 421 SMNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAY 480
           SMNILSNLLSISTLFIFMLVAI LIIRRYYSS ETTTS+RNKLLIYLVLIIGSS+ATAAY
Sbjct: 421 SMNILSNLLSISTLFIFMLVAIGLIIRRYYSSGETTTSDRNKLLIYLVLIIGSSLATAAY 480

Query: 481 WSTSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSI 540
           WSTSDNWIG+AVTLPIWF STLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSI
Sbjct: 481 WSTSDNWIGFAVTLPIWFLSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSI 540

Query: 541 DRASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV 585
           DRASFERFGIWTGILLIYYFLFGLHASYDTAM SKTKTAE NDSV
Sbjct: 541 DRASFERFGIWTGILLIYYFLFGLHASYDTAMASKTKTAETNDSV 585

BLAST of CSPI05G30430 vs. ExPASy TrEMBL
Match: A0A5D3BMU5 (Cationic amino acid transporter 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001370 PE=4 SV=1)

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 563/585 (96.24%), Postives = 569/585 (97.26%), Query Frame = 0

Query: 1   MGQPSSDGSNG-EGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSAD 60
           MGQPSSDGSNG EGIRRR CGCGKDDFLPEESFQSWENYTKALKATP RLF+RLTARSAD
Sbjct: 1   MGQPSSDGSNGSEGIRRRFCGCGKDDFLPEESFQSWENYTKALKATPLRLFDRLTARSAD 60

Query: 61  HTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVS 120
           HTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVS
Sbjct: 61  HTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVS 120

Query: 121 GISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWT 180
           GISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWT
Sbjct: 121 GISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWT 180

Query: 181 SYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASIL 240
           SYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIV+ILIIG FALVSTKGSSRLNYVASIL
Sbjct: 181 SYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVIILIIGTFALVSTKGSSRLNYVASIL 240

Query: 241 HVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPA 300
           HVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIF ASAVLFFAYVGFDAVSTLAEETKNPA
Sbjct: 241 HVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFEASAVLFFAYVGFDAVSTLAEETKNPA 300

Query: 301 KDIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGA 360
           KDIPIGLVGSMVITT LYCILAVTLCLMQPYQ+IDADAPFSMAFEAVGWSWAKYIVAAGA
Sbjct: 301 KDIPIGLVGSMVITTFLYCILAVTLCLMQPYQKIDADAPFSMAFEAVGWSWAKYIVAAGA 360

Query: 361 IKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFT 420
           IKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATA ML ATA VAFFT
Sbjct: 361 IKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATAAMLTATATVAFFT 420

Query: 421 SMNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAY 480
           SMNILSNLLSISTLFIFMLVAI LIIRRYYSS ETTTS+RNKLLIYLVLIIGSS+ATAAY
Sbjct: 421 SMNILSNLLSISTLFIFMLVAIGLIIRRYYSSGETTTSDRNKLLIYLVLIIGSSLATAAY 480

Query: 481 WSTSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSI 540
           WSTSDNWIG+AVTLPIWF STLALWL VPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSI
Sbjct: 481 WSTSDNWIGFAVTLPIWFLSTLALWLSVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSI 540

Query: 541 DRASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV 585
           DRASFERFGIWTGILLIYYFLFGLHASYDTAM SKTKTAE ND V
Sbjct: 541 DRASFERFGIWTGILLIYYFLFGLHASYDTAMASKTKTAETNDRV 585

BLAST of CSPI05G30430 vs. ExPASy TrEMBL
Match: A0A6J1K040 (cationic amino acid transporter 1-like OS=Cucurbita maxima OX=3661 GN=LOC111490398 PE=4 SV=1)

HSP 1 Score: 995.7 bits (2573), Expect = 7.9e-287
Identity = 492/584 (84.25%), Postives = 538/584 (92.12%), Query Frame = 0

Query: 1   MGQPSSDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADH 60
           MGQP+SD   GE IRRRSCGC K DFLPEESF+SW NYT+ALKATP RL +RLTARS +H
Sbjct: 1   MGQPNSDAGGGEEIRRRSCGCSKQDFLPEESFKSWGNYTEALKATPARLLDRLTARSGEH 60

Query: 61  TELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSG 120
           TELVEMKARSQH+MKK+LTWWDLMWFG+GAVIGAGIFVLTGLETKKHAGPAVVLSY+VSG
Sbjct: 61  TELVEMKARSQHEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAVVLSYVVSG 120

Query: 121 ISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTS 180
           +SAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEY+IG AAVAR+WTS
Sbjct: 121 VSAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTS 180

Query: 181 YFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILH 240
           YFATLCNHHPNDFR+H+S+FPDDYNQLDPIAIVVI+ I   A+ STKGSSR NY+ASI H
Sbjct: 181 YFATLCNHHPNDFRIHVSSFPDDYNQLDPIAIVVIIAICTLAVASTKGSSRFNYIASIFH 240

Query: 241 VVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAK 300
           V +I+FI+IAGLT+ANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVST+AEETKNPAK
Sbjct: 241 VAIILFIVIAGLTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAK 300

Query: 301 DIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAI 360
           DIPIGLVGSMVITT+ YCILAVTLCLMQPYQQID DAPFS+AFEAVGW WAKYIVAAGAI
Sbjct: 301 DIPIGLVGSMVITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAI 360

Query: 361 KGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTS 420
           KGMTTVLLV +VGQARYLTHIARTHM+SPWFAKVHE+TGTPVNATATML ATA++AFFTS
Sbjct: 361 KGMTTVLLVSAVGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTS 420

Query: 421 MNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYW 480
           + ILSNLLSISTLFIFMLVA+ LI+RRYY S ETT S+RNKL+I +VLI+GSS+ATA YW
Sbjct: 421 LGILSNLLSISTLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICIVLILGSSIATAVYW 480

Query: 481 STSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSID 540
            +SD WIG  V+  IWF STLALWLGVPQAKKP+VWGVPLVPW+PSLSIAIN FLLGSID
Sbjct: 481 GSSDGWIGLVVSNSIWFLSTLALWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSID 540

Query: 541 RASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV 585
           +ASFERFGIWTGILL+YYFL GLHASYD+ M SKT   E+N SV
Sbjct: 541 KASFERFGIWTGILLVYYFLIGLHASYDSVMESKTVAGEVNSSV 584

BLAST of CSPI05G30430 vs. ExPASy TrEMBL
Match: A0A6J1K6G4 (cationic amino acid transporter 1-like OS=Cucurbita maxima OX=3661 GN=LOC111490510 PE=4 SV=1)

HSP 1 Score: 992.6 bits (2565), Expect = 6.7e-286
Identity = 491/584 (84.08%), Postives = 537/584 (91.95%), Query Frame = 0

Query: 1   MGQPSSDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADH 60
           MGQ + D   GEGIRRRSC C K+DFLPEESF+SW NYTKALKATP RL +RLTARS +H
Sbjct: 1   MGQSNGDVGGGEGIRRRSCSCSKEDFLPEESFKSWGNYTKALKATPARLLDRLTARSGEH 60

Query: 61  TELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSG 120
           TELVEMKARSQH+MKK+LTWWDLMWFG+GAVIGAGIFVLTGLETKKHAGPAVVLSY+VSG
Sbjct: 61  TELVEMKARSQHEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAVVLSYVVSG 120

Query: 121 ISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTS 180
           +SAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEY+IG AAVAR+WTS
Sbjct: 121 VSAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTS 180

Query: 181 YFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILH 240
           YFATLCNHHPNDFR+H+S+FPDDYNQLDPIAIVVI+ I   A+ STKGSSR NY+ASI H
Sbjct: 181 YFATLCNHHPNDFRIHVSSFPDDYNQLDPIAIVVIIAICTLAVASTKGSSRFNYIASIFH 240

Query: 241 VVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAK 300
           V +I+FI+IAGLT+ANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVST+AEETKNPAK
Sbjct: 241 VAIILFIVIAGLTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAK 300

Query: 301 DIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAI 360
           DIPIGLVGSMVITT+ YCILAVTLCLMQPYQQID DAPFS+AFEAVGW WAKYIVAAGAI
Sbjct: 301 DIPIGLVGSMVITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAI 360

Query: 361 KGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTS 420
           KGMTTVLLV +VGQARYLTHIARTHM+SPWFAKVHE+TGTPVNATATML ATA++AFFTS
Sbjct: 361 KGMTTVLLVNAVGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTS 420

Query: 421 MNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYW 480
           + ILSNLLSISTLFIFMLVA+ LI+RRYY S ETT S+RNKL+I +VLI+GSS+ATA YW
Sbjct: 421 LGILSNLLSISTLFIFMLVAVGLIVRRYYVSGETTPSDRNKLVICIVLILGSSIATAVYW 480

Query: 481 STSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSID 540
            +SD WIG  V+  IWF STLALWLGVPQAKKP+VWGVPLVPW+PSLSIAIN FLLGSID
Sbjct: 481 GSSDGWIGLVVSNSIWFLSTLALWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSID 540

Query: 541 RASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV 585
           +ASFERFGIWTGILL+YYFL GLHASYD+ M SKT   E+N SV
Sbjct: 541 KASFERFGIWTGILLVYYFLIGLHASYDSVMESKTVAGEVNSSV 584

BLAST of CSPI05G30430 vs. NCBI nr
Match: XP_004141615.1 (cationic amino acid transporter 1 [Cucumis sativus] >XP_031741583.1 cationic amino acid transporter 1 [Cucumis sativus] >KGN52612.1 hypothetical protein Csa_008097 [Cucumis sativus])

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 582/584 (99.66%), Postives = 582/584 (99.66%), Query Frame = 0

Query: 1   MGQPSSDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADH 60
           MGQPSSDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADH
Sbjct: 1   MGQPSSDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADH 60

Query: 61  TELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSG 120
           TELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSG
Sbjct: 61  TELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSG 120

Query: 121 ISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTS 180
           ISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTS
Sbjct: 121 ISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTS 180

Query: 181 YFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILH 240
           YFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILH
Sbjct: 181 YFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILH 240

Query: 241 VVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAK 300
           VVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAK
Sbjct: 241 VVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAK 300

Query: 301 DIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAI 360
           DIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAI
Sbjct: 301 DIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAI 360

Query: 361 KGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTS 420
           KGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTS
Sbjct: 361 KGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTS 420

Query: 421 MNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYW 480
           MNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLI LVLIIGSSMATA YW
Sbjct: 421 MNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLICLVLIIGSSMATATYW 480

Query: 481 STSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSID 540
           STSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSID
Sbjct: 481 STSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSID 540

Query: 541 RASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV 585
           RASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV
Sbjct: 541 RASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV 584

BLAST of CSPI05G30430 vs. NCBI nr
Match: XP_008459518.1 (PREDICTED: cationic amino acid transporter 1-like [Cucumis melo])

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 565/585 (96.58%), Postives = 571/585 (97.61%), Query Frame = 0

Query: 1   MGQPSSDGSNG-EGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSAD 60
           MGQPSSDGSNG EGIRRR CGCGKDDFLPEESFQSWENYTKALKATP RLF+RLTARSAD
Sbjct: 1   MGQPSSDGSNGSEGIRRRFCGCGKDDFLPEESFQSWENYTKALKATPLRLFDRLTARSAD 60

Query: 61  HTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVS 120
           HTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVS
Sbjct: 61  HTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVS 120

Query: 121 GISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWT 180
           GISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWT
Sbjct: 121 GISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWT 180

Query: 181 SYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASIL 240
           SYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIV+ILIIG FALVSTKGSSRLNYVASIL
Sbjct: 181 SYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVIILIIGTFALVSTKGSSRLNYVASIL 240

Query: 241 HVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPA 300
           HVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIF ASAVLFFAYVGFDAVSTLAEETKNPA
Sbjct: 241 HVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFEASAVLFFAYVGFDAVSTLAEETKNPA 300

Query: 301 KDIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGA 360
           KDIPIGLVGSMVITT LYCILAVTLCLMQPYQ+IDADAPFSMAFEAVGWSWAKYIVAAGA
Sbjct: 301 KDIPIGLVGSMVITTFLYCILAVTLCLMQPYQKIDADAPFSMAFEAVGWSWAKYIVAAGA 360

Query: 361 IKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFT 420
           IKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATA ML ATA VAFFT
Sbjct: 361 IKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATAAMLAATATVAFFT 420

Query: 421 SMNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAY 480
           SMNILSNLLSISTLFIFMLVAI LIIRRYYSS ETTTS+RNKLLIYLVLIIGSS+ATAAY
Sbjct: 421 SMNILSNLLSISTLFIFMLVAIGLIIRRYYSSGETTTSDRNKLLIYLVLIIGSSLATAAY 480

Query: 481 WSTSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSI 540
           WSTSDNWIG+AVTLPIWF STLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSI
Sbjct: 481 WSTSDNWIGFAVTLPIWFLSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSI 540

Query: 541 DRASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV 585
           DRASFERFGIWTGILLIYYFLFGLHASYDTAM SKTKTAE NDSV
Sbjct: 541 DRASFERFGIWTGILLIYYFLFGLHASYDTAMASKTKTAETNDSV 585

BLAST of CSPI05G30430 vs. NCBI nr
Match: KAA0039344.1 (cationic amino acid transporter 1-like [Cucumis melo var. makuwa] >TYK00527.1 cationic amino acid transporter 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 563/585 (96.24%), Postives = 569/585 (97.26%), Query Frame = 0

Query: 1   MGQPSSDGSNG-EGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSAD 60
           MGQPSSDGSNG EGIRRR CGCGKDDFLPEESFQSWENYTKALKATP RLF+RLTARSAD
Sbjct: 1   MGQPSSDGSNGSEGIRRRFCGCGKDDFLPEESFQSWENYTKALKATPLRLFDRLTARSAD 60

Query: 61  HTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVS 120
           HTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVS
Sbjct: 61  HTELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVS 120

Query: 121 GISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWT 180
           GISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWT
Sbjct: 121 GISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWT 180

Query: 181 SYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASIL 240
           SYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIV+ILIIG FALVSTKGSSRLNYVASIL
Sbjct: 181 SYFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVIILIIGTFALVSTKGSSRLNYVASIL 240

Query: 241 HVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPA 300
           HVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIF ASAVLFFAYVGFDAVSTLAEETKNPA
Sbjct: 241 HVVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFEASAVLFFAYVGFDAVSTLAEETKNPA 300

Query: 301 KDIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGA 360
           KDIPIGLVGSMVITT LYCILAVTLCLMQPYQ+IDADAPFSMAFEAVGWSWAKYIVAAGA
Sbjct: 301 KDIPIGLVGSMVITTFLYCILAVTLCLMQPYQKIDADAPFSMAFEAVGWSWAKYIVAAGA 360

Query: 361 IKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFT 420
           IKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATA ML ATA VAFFT
Sbjct: 361 IKGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATAAMLTATATVAFFT 420

Query: 421 SMNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAY 480
           SMNILSNLLSISTLFIFMLVAI LIIRRYYSS ETTTS+RNKLLIYLVLIIGSS+ATAAY
Sbjct: 421 SMNILSNLLSISTLFIFMLVAIGLIIRRYYSSGETTTSDRNKLLIYLVLIIGSSLATAAY 480

Query: 481 WSTSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSI 540
           WSTSDNWIG+AVTLPIWF STLALWL VPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSI
Sbjct: 481 WSTSDNWIGFAVTLPIWFLSTLALWLSVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSI 540

Query: 541 DRASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV 585
           DRASFERFGIWTGILLIYYFLFGLHASYDTAM SKTKTAE ND V
Sbjct: 541 DRASFERFGIWTGILLIYYFLFGLHASYDTAMASKTKTAETNDRV 585

BLAST of CSPI05G30430 vs. NCBI nr
Match: XP_038891137.1 (cationic amino acid transporter 1-like [Benincasa hispida])

HSP 1 Score: 1050.8 bits (2716), Expect = 4.3e-303
Identity = 530/584 (90.75%), Postives = 558/584 (95.55%), Query Frame = 0

Query: 1   MGQPSSDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADH 60
           M QP SD + GEGIRRRSCGCGK+DFLPEESFQSWENYTKALKATP RL +RLTARSADH
Sbjct: 1   MEQPISDANGGEGIRRRSCGCGKEDFLPEESFQSWENYTKALKATPVRLLDRLTARSADH 60

Query: 61  TELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSG 120
           TELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSY+VSG
Sbjct: 61  TELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYVVSG 120

Query: 121 ISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTS 180
           ISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNI+LEYIIGSAAVAR+WTS
Sbjct: 121 ISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNIILEYIIGSAAVARSWTS 180

Query: 181 YFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILH 240
           YFATLCNHHPNDFRVH+ AFPDDYNQLDPIAIVVILIIG FA+VSTKGSSRLNYVASILH
Sbjct: 181 YFATLCNHHPNDFRVHVPAFPDDYNQLDPIAIVVILIIGTFAMVSTKGSSRLNYVASILH 240

Query: 241 VVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAK 300
           VVVI+FIIIAGLT+AN KNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAK
Sbjct: 241 VVVILFIIIAGLTKANSKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAK 300

Query: 301 DIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAI 360
           DIPIGLVGSMV+TT  YCILAVTLCLMQPYQQIDADAPFSMAFEAVGW WAKYIVAAGAI
Sbjct: 301 DIPIGLVGSMVLTTFAYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWGWAKYIVAAGAI 360

Query: 361 KGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTS 420
           KGMTTVLLV SVGQARYLTHIARTHMVSPWFAKV+EKTGTPVNATA+MLIATA++AFFT+
Sbjct: 361 KGMTTVLLVNSVGQARYLTHIARTHMVSPWFAKVNEKTGTPVNATASMLIATAIIAFFTT 420

Query: 421 MNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYW 480
           M ILSNLLSI+TLFIFMLVAI LI+RRYY+SSETTTS+RNKL+I LVLIIGSS+ATAAYW
Sbjct: 421 MEILSNLLSIATLFIFMLVAIGLIVRRYYNSSETTTSDRNKLIICLVLIIGSSIATAAYW 480

Query: 481 STSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSID 540
            TSD W+G+AVT+P WF STLALWLGVPQAKKPQVWGVP VPWLPS+SIAINFFLLGSID
Sbjct: 481 GTSDGWVGFAVTIPTWFLSTLALWLGVPQAKKPQVWGVPFVPWLPSISIAINFFLLGSID 540

Query: 541 RASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV 585
           +ASFERFGIWTGILLIYYFLFGLHASYD AM SKT+  EIN SV
Sbjct: 541 KASFERFGIWTGILLIYYFLFGLHASYDIAMESKTQAREINGSV 584

BLAST of CSPI05G30430 vs. NCBI nr
Match: XP_022994771.1 (cationic amino acid transporter 1-like [Cucurbita maxima])

HSP 1 Score: 995.7 bits (2573), Expect = 1.6e-286
Identity = 492/584 (84.25%), Postives = 538/584 (92.12%), Query Frame = 0

Query: 1   MGQPSSDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADH 60
           MGQP+SD   GE IRRRSCGC K DFLPEESF+SW NYT+ALKATP RL +RLTARS +H
Sbjct: 1   MGQPNSDAGGGEEIRRRSCGCSKQDFLPEESFKSWGNYTEALKATPARLLDRLTARSGEH 60

Query: 61  TELVEMKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSG 120
           TELVEMKARSQH+MKK+LTWWDLMWFG+GAVIGAGIFVLTGLETKKHAGPAVVLSY+VSG
Sbjct: 61  TELVEMKARSQHEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAVVLSYVVSG 120

Query: 121 ISAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTS 180
           +SAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEY+IG AAVAR+WTS
Sbjct: 121 VSAMLSVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTS 180

Query: 181 YFATLCNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILH 240
           YFATLCNHHPNDFR+H+S+FPDDYNQLDPIAIVVI+ I   A+ STKGSSR NY+ASI H
Sbjct: 181 YFATLCNHHPNDFRIHVSSFPDDYNQLDPIAIVVIIAICTLAVASTKGSSRFNYIASIFH 240

Query: 241 VVVIVFIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAK 300
           V +I+FI+IAGLT+ANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVST+AEETKNPAK
Sbjct: 241 VAIILFIVIAGLTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAK 300

Query: 301 DIPIGLVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAI 360
           DIPIGLVGSMVITT+ YCILAVTLCLMQPYQQID DAPFS+AFEAVGW WAKYIVAAGAI
Sbjct: 301 DIPIGLVGSMVITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAI 360

Query: 361 KGMTTVLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTS 420
           KGMTTVLLV +VGQARYLTHIARTHM+SPWFAKVHE+TGTPVNATATML ATA++AFFTS
Sbjct: 361 KGMTTVLLVSAVGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTS 420

Query: 421 MNILSNLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYW 480
           + ILSNLLSISTLFIFMLVA+ LI+RRYY S ETT S+RNKL+I +VLI+GSS+ATA YW
Sbjct: 421 LGILSNLLSISTLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICIVLILGSSIATAVYW 480

Query: 481 STSDNWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSID 540
            +SD WIG  V+  IWF STLALWLGVPQAKKP+VWGVPLVPW+PSLSIAIN FLLGSID
Sbjct: 481 GSSDGWIGLVVSNSIWFLSTLALWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSID 540

Query: 541 RASFERFGIWTGILLIYYFLFGLHASYDTAMNSKTKTAEINDSV 585
           +ASFERFGIWTGILL+YYFL GLHASYD+ M SKT   E+N SV
Sbjct: 541 KASFERFGIWTGILLVYYFLIGLHASYDSVMESKTVAGEVNSSV 584

BLAST of CSPI05G30430 vs. TAIR 10
Match: AT4G21120.1 (amino acid transporter 1 )

HSP 1 Score: 823.2 bits (2125), Expect = 1.4e-238
Identity = 401/566 (70.85%), Postives = 478/566 (84.45%), Query Frame = 0

Query: 6   SDGSNGEGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADHTELVE 65
           + G   +G+RRR C C KDDFLPEESFQS  NY KALK TP R  +R+  RS D  E+ E
Sbjct: 2   ASGGGDDGLRRRGCSCTKDDFLPEESFQSMGNYLKALKETPSRFMDRIMTRSLDSDEINE 61

Query: 66  MKARSQHQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSGISAML 125
           MKARS H+MKKTLTWWDLMWFG+GAVIG+GIFVLTGLE + H+GPAVVLSY+VSG+SAML
Sbjct: 62  MKARSGHEMKKTLTWWDLMWFGIGAVIGSGIFVLTGLEARNHSGPAVVLSYVVSGVSAML 121

Query: 126 SVFCYTEFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTSYFATL 185
           SVFCYTEFAVEIP AGGSFAYLRVELGDF+AFIAAGNI+LEY++G AAVAR+WTSYFATL
Sbjct: 122 SVFCYTEFAVEIPVAGGSFAYLRVELGDFMAFIAAGNIILEYVVGGAAVARSWTSYFATL 181

Query: 186 CNHHPNDFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILHVVVIV 245
            NH P DFR+ +    +DY+ LDPIA+ V  II + A+V TKGSSR NY+ASI+H+VVI+
Sbjct: 182 LNHKPEDFRIIVHKLGEDYSHLDPIAVGVCAIICVLAVVGTKGSSRFNYIASIIHMVVIL 241

Query: 246 FIIIAGLTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAKDIPIG 305
           F+IIAG T+A+ KN++ F PYG RG+F ++AVLFFAY+GFDAVST+AEETKNP +DIPIG
Sbjct: 242 FVIIAGFTKADVKNYSDFTPYGVRGVFKSAAVLFFAYIGFDAVSTMAEETKNPGRDIPIG 301

Query: 306 LVGSMVITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAIKGMTT 365
           LVGSMV+TTV YC++AVTLCLMQPYQQID DAPFS+AF AVGW WAKYIVA GA+KGMTT
Sbjct: 302 LVGSMVVTTVCYCLMAVTLCLMQPYQQIDPDAPFSVAFSAVGWDWAKYIVAFGALKGMTT 361

Query: 366 VLLVGSVGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTSMNILS 425
           VLLVG++GQARY+THIAR HM+ PW A+V+ KTGTP+NAT  ML ATA++AFFT + IL+
Sbjct: 362 VLLVGAIGQARYMTHIARAHMMPPWLAQVNAKTGTPINATVVMLAATALIAFFTKLKILA 421

Query: 426 NLLSISTLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYWS-TSD 485
           +LLS+STLFIFM VA+AL++RRYY + ET+T +RNK L++L LI+ SS ATA YW+   +
Sbjct: 422 DLLSVSTLFIFMFVAVALLVRRYYVTGETSTRDRNKFLVFLGLILASSTATAVYWALEEE 481

Query: 486 NWIGYAVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSIDRASF 545
            WIGY +T+PIWF ST+A+   VPQA+ P++WGVPLVPWLPS SIAIN FLLGSID  SF
Sbjct: 482 GWIGYCITVPIWFLSTVAMKFLVPQARAPKIWGVPLVPWLPSASIAINIFLLGSIDTKSF 541

Query: 546 ERFGIWTGILLIYYFLFGLHASYDTA 571
            RF IWTGILLIYY LFGLHA+YDTA
Sbjct: 542 VRFAIWTGILLIYYVLFGLHATYDTA 567

BLAST of CSPI05G30430 vs. TAIR 10
Match: AT2G34960.1 (cationic amino acid transporter 5 )

HSP 1 Score: 654.1 bits (1686), Expect = 1.1e-187
Identity = 330/561 (58.82%), Postives = 425/561 (75.76%), Query Frame = 0

Query: 12  EGIRRRSCGCGKDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADHTELVEMKARSQ 71
           EG  R      K DF PEESFQS+ +Y  AL  T  R  NRL +RS D  E  E+K +S+
Sbjct: 2   EGEERGYWRWSKRDFFPEESFQSFGSYRAALSQTCSRFKNRLVSRSDDENERFELKKQSE 61

Query: 72  HQMKKTLTWWDLMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSGISAMLSVFCYT 131
           H+MK+ LTWWDL+WFG G+VIGAGIFVLTG E  + AGPA+VLSY+VSG+SAMLSVFCYT
Sbjct: 62  HEMKRCLTWWDLVWFGFGSVIGAGIFVLTGQEAHEQAGPAIVLSYVVSGLSAMLSVFCYT 121

Query: 132 EFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTSYFATLCNHHPN 191
           EFAVEIP AGGSFAYLR+ELGDF AFI AGNILLE I+G+AAVARAWTSYFATL N  PN
Sbjct: 122 EFAVEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFATLLNRSPN 181

Query: 192 DFRVHISAFPDDYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILHVVVIVFIIIAG 251
             R+  +     +N LDPIA+VVI      A +ST+ +S LN++AS ++ +VI F+IIAG
Sbjct: 182 ALRIK-TDLSSGFNLLDPIAVVVIAASATIASISTRKTSLLNWIASAINTLVIFFVIIAG 241

Query: 252 LTQANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAKDIPIGLVGSMV 311
              A+  N TPF P+GP G+F A+AV++FAY GFD+++T+AEETKNP++DIPIGL+GSM 
Sbjct: 242 FIHADTSNLTPFLPFGPEGVFRAAAVVYFAYGGFDSIATMAEETKNPSRDIPIGLLGSMS 301

Query: 312 ITTVLYCILAVTLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAIKGMTTVLLVGS 371
           I TV+YC++A++L +MQ Y  ID +A +S+AF++VG  W KY+VA GA+KGMTTVLLVG+
Sbjct: 302 IITVIYCLMALSLSMMQKYTDIDPNAAYSVAFQSVGMKWGKYLVALGALKGMTTVLLVGA 361

Query: 372 VGQARYLTHIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTSMNILSNLLSIS 431
           +GQARY+THIARTHM+ P FA VH KTGTP+NA   + I +A++AFF+ +++L++LLSIS
Sbjct: 362 LGQARYVTHIARTHMIPPIFALVHPKTGTPINANLLVAIPSALIAFFSGLDVLASLLSIS 421

Query: 432 TLFIFMLVAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYW--STSDNWIGY 491
           TLFIF ++ IAL++RRYY   +T   +  KL+  L+ ++ SSM T+AYW      +WIGY
Sbjct: 422 TLFIFTMMPIALLVRRYYVRQDTPRVHLIKLITCLLFVVVSSMGTSAYWGMQRKGSWIGY 481

Query: 492 AVTLPIWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSIDRASFERFGI 551
            VT+P WF  TL +   VPQ + P+VWGVPLVPWLP LSIA N FL+GS+   +F RFG+
Sbjct: 482 TVTVPFWFLGTLGIVFFVPQQRTPKVWGVPLVPWLPCLSIATNIFLMGSLGAMAFVRFGV 541

Query: 552 WTGILLIYYFLFGLHASYDTA 571
            T  +L+YYFL GLHA++D A
Sbjct: 542 CTLAMLLYYFLLGLHATFDMA 561

BLAST of CSPI05G30430 vs. TAIR 10
Match: AT1G17120.1 (cationic amino acid transporter 8 )

HSP 1 Score: 577.4 bits (1487), Expect = 1.3e-164
Identity = 289/549 (52.64%), Postives = 399/549 (72.68%), Query Frame = 0

Query: 23  KDDFLPEESFQSWENYTKALKATPFRLFNRLTARSADHTELVEMKARSQHQMKKTLTWWD 82
           K DF PE SFQS+  Y  AL AT  RL +RL +RS+D  EL   +  S++ M++ LTWWD
Sbjct: 30  KQDFFPEFSFQSFSTYKSALSATCPRLADRLLSRSSDAYELDAARRESENPMRRCLTWWD 89

Query: 83  LMWFGVGAVIGAGIFVLTGLETKKHAGPAVVLSYIVSGISAMLSVFCYTEFAVEIPAAGG 142
           L+W   G+V+G+G+FV+TG E +  AGPAVVLSY +SG+SA+LSV CY EF VEIP AGG
Sbjct: 90  LLWLSFGSVVGSGVFVITGQEARVGAGPAVVLSYAISGVSALLSVLCYAEFGVEIPVAGG 149

Query: 143 SFAYLRVELGDFVAFIAAGNILLEYIIGSAAVARAWTSYFATLCNHHPNDFRVHISAFPD 202
           SF+YLRVELGDF+AFIAAGNILLE ++G+A + R+W+SY A+L  +  + FR+ + +F  
Sbjct: 150 SFSYLRVELGDFIAFIAAGNILLEAMVGAAGLGRSWSSYLASLVKNDSDYFRIKVDSFAK 209

Query: 203 DYNQLDPIAIVVILIIGIFALVSTKGSSRLNYVASILHVVVIVFIIIAGLTQANPKNFTP 262
            ++ LDP+A+ V+L+    A+  TK +S LN + S++ V +IVFI++ G T +   N  P
Sbjct: 210 GFDLLDPVAVAVLLVANGIAMTGTKRTSWLNLITSMVTVCIIVFIVVVGFTHSKTSNLVP 269

Query: 263 FAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAKDIPIGLVGSMVITTVLYCILAV 322
           F PYG +G+  ++AV++++Y GFD V+ +AEET+ P++DIPIGLVGSM + TV+YC++A+
Sbjct: 270 FFPYGAKGVVQSAAVVYWSYTGFDMVANMAEETEKPSRDIPIGLVGSMSMITVVYCLMAL 329

Query: 323 TLCLMQPYQQIDADAPFSMAFEAVGWSWAKYIVAAGAIKGMTTVLLVGSVGQARYLTHIA 382
            L +M  Y +IDA+A +S+AF  +G  WAKY+V   A+KGMTT LLVGS+GQARY T IA
Sbjct: 330 ALTMMVKYTEIDANAAYSVAFAQIGMKWAKYLVGICALKGMTTSLLVGSLGQARYTTQIA 389

Query: 383 RTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTSMNILSNLLSISTLFIFMLVAIA 442
           R+HM+ PWFA VH KTGTP+ AT  + I +++++FFTS+ +LS++ S +TLFIFMLVA+A
Sbjct: 390 RSHMIPPWFALVHPKTGTPIYATLLVTILSSIISFFTSLEVLSSVFSFATLFIFMLVAVA 449

Query: 443 LIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYWSTS-DNWIGYAVTLPIWFFSTL 502
           L++RRYY    T  +   K L +L LII SS+  +A W++    WI Y VT  IWF  TL
Sbjct: 450 LLVRRYYVKDVTPEAGLLKFLGFLFLIIASSIGVSALWNSGVKGWIAYTVTGVIWFIGTL 509

Query: 503 ALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSIDRASFERFGIWTGILLIYYFLF 562
            L L +P+ + P+VWGVPLVPWLPS SIA+N FL+GS+   +F RF I T ++L+YY   
Sbjct: 510 GLAL-LPKYRVPKVWGVPLVPWLPSFSIAMNLFLIGSLGYVAFLRFIICTMVMLLYYLFV 569

Query: 563 GLHASYDTA 571
           GLHA+YD A
Sbjct: 570 GLHATYDVA 577

BLAST of CSPI05G30430 vs. TAIR 10
Match: AT5G04770.1 (cationic amino acid transporter 6 )

HSP 1 Score: 450.3 bits (1157), Expect = 2.4e-126
Identity = 234/556 (42.09%), Postives = 355/556 (63.85%), Query Frame = 0

Query: 31  SFQSWENYTKALKATPFRLFNRLTARSADHTELVEMKARSQHQMKKTLTWWDLMWFGVGA 90
           SF S   Y  +L ATP RL  R  + S    E+  ++A S  QM++TL W+DL+  G+G 
Sbjct: 14  SFSSLRVYLNSLSATPSRLSRRAISVSTSSDEMSRVRAVSGEQMRRTLRWYDLIGLGIGG 73

Query: 91  VIGAGIFVLTGLETKKHAGPAVVLSYIVSGISAMLSVFCYTEFAVEIPAAGGSFAYLRVE 150
           ++GAG+FV TG  ++  AGP++V+SY ++G+ A+LS FCYTEFAV +P AGG+F+Y+R+ 
Sbjct: 74  MVGAGVFVTTGRASRLDAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRIT 133

Query: 151 LGDFVAFIAAGNILLEYIIGSAAVARAWTSYFATLCNHHPNDFRVHISAFPDDYNQLDPI 210
            G+F AF    N++++Y++ +AAV+R++T+Y  T      + +R  +S  P  +N++DP+
Sbjct: 134 FGEFPAFFTGANLVMDYVMSNAAVSRSFTAYLGTAFGISTSKWRFVVSGLPKGFNEIDPV 193

Query: 211 AIVVILIIGIFALVSTKGSSRLNYVASILHVVVIVFIIIAGL---------TQANPKNFT 270
           A++V+L+I +    ST+ SS++N + +  H+  I F+I+ G          + ANP++ +
Sbjct: 194 AVLVVLVITVIICCSTRESSKVNMIMTAFHIAFIFFVIVMGFIKGDSKNLSSPANPEHPS 253

Query: 271 PFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAKDIPIGLVGSMVITTVLYCILA 330
            F P+G  G+F  +A+++ +Y+G+DAVST+AEE +NP KDIP+G+ GS+ I TVLYC++A
Sbjct: 254 GFFPFGAAGVFNGAAMVYLSYIGYDAVSTMAEEVENPVKDIPVGVSGSVAIVTVLYCLMA 313

Query: 331 VTLCLMQPYQQIDADAPFSMAFE-AVGWSWAKYIVAAGAIKGMTTVLLVGSVGQARYLTH 390
           V++ ++ PY  ID +APFS AF  + GW W   +V  GA  G+ T LLV  +GQARY+  
Sbjct: 314 VSMSMLLPYDLIDPEAPFSAAFRGSNGWEWVTKVVGIGASFGILTSLLVAMLGQARYMCV 373

Query: 391 IARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTSMNILSNLLSISTLFIFMLVA 450
           I R+ +V  WFAK+H KT TPVNA+  + I TA +A FT +N+L NL+SI TLF+F +VA
Sbjct: 374 IGRSRVVPFWFAKIHPKTSTPVNASTFLGIFTAALALFTDLNVLLNLVSIGTLFVFYMVA 433

Query: 451 IALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYWSTSDNWIGYAVTLPIWFFST 510
            ALI RRY     T        L +L L   +S+     W         A  L       
Sbjct: 434 NALIFRRYVPVGPTKPW---PTLCFLTLFSITSLVFTLIWKLVPEGKPKAFMLGASAVVA 493

Query: 511 LALWLG----VPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSIDRASFERFGIWTGILLI 570
           +A+ L     VPQA+KP++WGVP +PW P +SI +N FLLGS+D  S+ RFG ++G++++
Sbjct: 494 IAIVLSFQCVVPQARKPELWGVPFMPWTPCVSIFLNIFLLGSLDAPSYVRFGFFSGLIVL 553

Query: 571 YYFLFGLHASYDTAMN 573
            Y  +G+HAS D   N
Sbjct: 554 VYLFYGVHASSDAEAN 566

BLAST of CSPI05G30430 vs. TAIR 10
Match: AT3G10600.1 (cationic amino acid transporter 7 )

HSP 1 Score: 447.2 bits (1149), Expect = 2.0e-125
Identity = 231/554 (41.70%), Postives = 357/554 (64.44%), Query Frame = 0

Query: 29  EESFQSWENYTKALKATPFRLFNRLTARSADHTELVEMKARSQHQMKKTLTWWDLMWFGV 88
           + SF S   Y  +L  TP R   R  + S  + E+  ++A S  QM++TL W+DL+  G+
Sbjct: 11  DTSFSSLRVYLNSLSDTPSRFSRRAVSVSTSYDEMSRVRAVSGEQMRRTLRWYDLIGLGI 70

Query: 89  GAVIGAGIFVLTGLETKKHAGPAVVLSYIVSGISAMLSVFCYTEFAVEIPAAGGSFAYLR 148
           G +IGAG+FV TG  ++ +AGP++V+SY ++G+ A+LS FCYTEFAV +P AGG+F+Y+R
Sbjct: 71  GGMIGAGVFVTTGRASRLYAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIR 130

Query: 149 VELGDFVAFIAAGNILLEYIIGSAAVARAWTSYFATLCNHHPNDFRVHISAFPDDYNQLD 208
           +  G+F AFI   N++++Y++ +AAV+R +T+Y  +      +++R  +S  P+ +N++D
Sbjct: 131 ITFGEFPAFITGANLIMDYVLSNAAVSRGFTAYLGSAFGISTSEWRFIVSGLPNGFNEID 190

Query: 209 PIAIVVILIIGIFALVSTKGSSRLNYVASILHVVVIVFIIIAGLTQA---------NPKN 268
           PIA++V+L +      ST+ SS++N V + LH+  IVF+I+ G ++          NP+N
Sbjct: 191 PIAVIVVLAVTFVICYSTRESSKVNMVLTALHIAFIVFVIVMGFSKGDVKNLTRPDNPEN 250

Query: 269 FTPFAPYGPRGIFVASAVLFFAYVGFDAVSTLAEETKNPAKDIPIGLVGSMVITTVLYCI 328
            + F P+G  G+F  +A+++ +Y+G+DAVST+AEE K+P KDIP+G+ GS+ I  VLYC+
Sbjct: 251 PSGFFPFGVSGVFNGAAMVYLSYIGYDAVSTMAEEVKDPVKDIPMGISGSVAIVIVLYCL 310

Query: 329 LAVTLCLMQPYQQIDADAPFSMAF-EAVGWSWAKYIVAAGAIKGMTTVLLVGSVGQARYL 388
           +A+++ ++ PY  IDA+AP+S AF ++ GW W   +V  GA  G+ T L+V  +GQARY+
Sbjct: 311 MAISMSMLLPYDLIDAEAPYSAAFSKSEGWEWVTRVVGIGASFGILTSLIVAMLGQARYM 370

Query: 389 THIARTHMVSPWFAKVHEKTGTPVNATATMLIATAMVAFFTSMNILSNLLSISTLFIFML 448
             I R+ +V  WFAKVH KT TPVNA+A + I TA++A FT +N+L NL+SI TLF+F +
Sbjct: 371 CVIGRSRVVPIWFAKVHPKTSTPVNASAFLGIFTAVLALFTDLNVLLNLVSIGTLFVFYM 430

Query: 449 VAIALIIRRYYSSSETTTSNRNKLLIYLVLIIGSSMATAAYW----STSDNWIGYAVTLP 508
           VA A+I RRY +   T        L +L L   +S+     W    S    W     +  
Sbjct: 431 VANAVIFRRYVTVGYTEPW---PTLSFLCLFSITSILFTLVWQLAPSGPPKWFILGASTV 490

Query: 509 IWFFSTLALWLGVPQAKKPQVWGVPLVPWLPSLSIAINFFLLGSIDRASFERFGIWTGIL 568
                       VPQA+ P+ WGVPL+PW P +SI +N FLLGS+D  S+ RFG ++G++
Sbjct: 491 TAIAIVQIFHCVVPQARIPEFWGVPLMPWTPCVSIFLNIFLLGSLDAPSYIRFGFFSGLV 550

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84MA51.9e-23770.85Cationic amino acid transporter 1 OS=Arabidopsis thaliana OX=3702 GN=CAT1 PE=1 S... [more]
O647591.5e-18658.82Cationic amino acid transporter 5 OS=Arabidopsis thaliana OX=3702 GN=CAT5 PE=1 S... [more]
Q9SHH01.8e-16352.64Cationic amino acid transporter 8, vacuolar OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9LZ203.4e-12542.09Cationic amino acid transporter 6, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q9SQZ02.9e-12441.70Cationic amino acid transporter 7, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A0A0KW030.0e+0099.66AA_permease_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6473... [more]
A0A1S3CBK90.0e+0096.58cationic amino acid transporter 1-like OS=Cucumis melo OX=3656 GN=LOC103498634 P... [more]
A0A5D3BMU50.0e+0096.24Cationic amino acid transporter 1-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1K0407.9e-28784.25cationic amino acid transporter 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114903... [more]
A0A6J1K6G46.7e-28684.08cationic amino acid transporter 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114905... [more]
Match NameE-valueIdentityDescription
XP_004141615.10.0e+0099.66cationic amino acid transporter 1 [Cucumis sativus] >XP_031741583.1 cationic ami... [more]
XP_008459518.10.0e+0096.58PREDICTED: cationic amino acid transporter 1-like [Cucumis melo][more]
KAA0039344.10.0e+0096.24cationic amino acid transporter 1-like [Cucumis melo var. makuwa] >TYK00527.1 ca... [more]
XP_038891137.14.3e-30390.75cationic amino acid transporter 1-like [Benincasa hispida][more]
XP_022994771.11.6e-28684.25cationic amino acid transporter 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G21120.11.4e-23870.85amino acid transporter 1 [more]
AT2G34960.11.1e-18758.82cationic amino acid transporter 5 [more]
AT1G17120.11.3e-16452.64cationic amino acid transporter 8 [more]
AT5G04770.12.4e-12642.09cationic amino acid transporter 6 [more]
AT3G10600.12.0e-12541.70cationic amino acid transporter 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029485Cationic amino acid transporter, C-terminalPFAMPF13906AA_permease_Ccoord: 516..566
e-value: 1.1E-16
score: 60.6
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 78..470
e-value: 3.0E-45
score: 154.9
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 72..512
e-value: 9.8E-97
score: 326.3
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 462..582
e-value: 1100.0
score: -4.8
coord: 56..515
e-value: 4.9E-69
score: 230.6
NoneNo IPR availablePANTHERPTHR43243INNER MEMBRANE TRANSPORTER YGJI-RELATEDcoord: 13..578
NoneNo IPR availablePANTHERPTHR43243:SF1CATIONIC AMINO ACID TRANSPORTER 1coord: 13..578

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G30430.1CSPI05G30430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015813 L-glutamate transmembrane transport
biological_process GO:1903401 L-lysine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005313 L-glutamate transmembrane transporter activity
molecular_function GO:0015189 L-lysine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity