CSPI05G30180 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G30180
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionAB hydrolase-1 domain-containing protein
LocationChr5: 28206279 .. 28210942 (-)
RNA-Seq ExpressionCSPI05G30180
SyntenyCSPI05G30180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTTCAACTACACAGACTCTATATATATAATACACATTTACAATCATTACTTCCAATTCGTAAATATTTGAAGAACCGCCATGGGAGAGAAAGAAAACCTGGCGCCAAGTTCAGTGAACTCTGTTTCAGTGCAGATTCCGCCGCACTTCGTTCTCGTTCATGGCATAAGTGGAGGAGGTTGGTGCTGGTACAAAATCAGATGTTTAATGGAGAATTCTGGCTTTAAAGTCACTTGTATTGATCTCAAAGGCGCTGGAATCGATCGCTCTGATCCGAATTCCGTCTTCAATTTCGACGATTACAATCAGCCTCTGCTCGATTTCATTTCTACTTTGCCGGAAAACGAACAGGTAATTAATGTTTTTATTATACGTTCGTCGTTTTCTTTTTTTTTGTAAATCTCTGTGTGGAATCCATCGTTCCGATCTGATTTCCGTCGTTAGATTAGACAATTACTATAAGCCTCTGCTCGATTTCATCTCTAGTTTAATAGAAAATGAGCAGGTAATTATATTATTATTATTATTATTATTATTATTATACATTCGTTGCTTCTTGTAATTTCATTTTCGTCTTCGGATGTGTCGTTCTTCAATCGATTGCGATCGAGTGAAGTTCTGTTTGATTTAGAGATCGTTGAAAACTTTTGCAGATCATTCTGGTTGGACATAGCGCTGGAGGATTAAGTGTTACTCAAGCGACGCTTAAGTTCGCGAAGAAAATCCGTTTGGCGGTGTATGTAGCGGCAACGATGCTGAGATTTGGATTCCAGAATGATCAAGATATTAAAGACGTAAGTAAATTCTCTTGTTCTCACTACTTTTAGTTCATGTTTGATTTTACGAATAGTAATTGAAGTAGAACTTGGAGTAGTAGTGCGGCGGGCGGACGACGAGATTGTTGTGAAGCTCGTCGCCGGCCAGCGGTGGGGGTGCTGGCGCTTTTGCTGGCGATTATATGCGAGTGAAAACGTGTGAATAGTTTTGGCCGTTAGTCGAACCGCTAAGCTCTCTTGCTCTCATCGCAGATGGCATGAGGCAGAGAAAACGCCCAGCCTGCTAATTCATTCGGTTAAATTAGAAATTTAGTTTATAAAATTTACAATTAGTCCCCATAATTTCAAATATTACAATATAGTCCTTAAAACGTAACAAGATTTTATAAACTATCTTTAAGAATCAATAAAATTCTCACTAATTAAATTTTAATTTTCTTTGAAACGAAATTTATAACTTATTATCATTAAAAAGCCATTAACTATTTATCGTTTTCTAGAAAGAAAAAAAAAAAAGAAGTCATTACTCAGTTAATTAAACACTTTTGTTTTTGTCTTTTTAATTAGGAAATGAAATAGAGACAGCCACCAGCCAGCTGGTTCATGTAAACTAAACTTTTATTAACATTTTGATTAAGGATTCCATTATATTAACCAATCCAAATCCAGATTGTAAGACTTAAATCAGTTAAATGTTAATTGTCCAAGTGATTACTTCATTCTCTTTATTAAATTTTTCATAATTTTGAAGATTTTTTCTTAAGAAATACCATAAATTAAAAGACATTGAATCATAAAAAATTAAATCCTTCCTACATTTAACAAGCAAACAATACCATTCATGAGGTTGAAGTTTAATTTCTACTAATGACAAAGATGTATGAAGAAGAATCTATCACATATCGGATTTTGATCGAAAACTTTCATATTAAATTGTAACATATTCTTGGTATTTAATATTTTTCTTGGACAAATATCTTTTTTAAAAAAAAAATAGTTCAATTTTTATAAAAAGAATTTTACTAGAAAAGAACTTTATTGAACAATTCGTTCGACGGAAGCGACACAGGAAGGATTGCGTCCAAGCTCATTCTAGAAAATAATTCAAAAGATTTTGTTGTCCACAAAAGACTGAAGCATGAAGTTTAAAAAAGAAAGATAATTTGAGTTGGGCATAAACATAATTCAAAAGACTAATAACCAAAATTGTGTTAGATTTATGTTTAAAGGAAAAGAGAAAGTACATATAAGTGATAAGTTGTGGTAATGTAATGTAAAAGTGGAGGTCGCTTGAATGCGATTTTTATCCCGAAGTAGATTGGTACGGTGGAACCACAGCGGCAGCCAAGAACGTGTCCCCCTCTCTTTGGTGAAAATACCTTCCTTTAGCTGCTGTAATTTTTTTGTGCCACACCCTACGTTGCGGATAAAAAGCTTTCTATGTAATTTTTTACAAAATAAAATACATTTTACTCCAACACATTTTCATAAATAAATAAATTAAAATATTTGCATTAGGGTAGCTAGACTACCCTACCTATATTAATATATATATATATAGTTAAACATTGTAGAAAGTTAAATGATCGTATGAAAAGTTACCACATCCTATAAAAAGTAAAGAATATTTGAAGGTAAAAAGTTAAACGGTCTGTATAAGAGGCAAACGATCAGATTGTTGAGTTGAAAGTGGAAAATATATATATAGTCTAACAACCCAACCCGACCCATATTTTACTGGTTGTGTTGAAAAATTAATCTTATTATTCGGAATGCCAATTCAACCATTCCGAAAATATGGGTTGGGTCGAGAGTGTCTCCCAACTCAATCCATTCACACCTCTAATTTACCTAACATACTTCTTATGCATCAAATAATAGGTATTATAGGTAGATTTAAAGTTTTCTCTTTTTTTCTTTTTAAATTTTAACTTAGATAAATTTAATATCTAATTAAGATTAAAACATTCCTTTAGAAGAATATAGAGTATATTAAAATGAAAATTAAGAATGTAATAAATCATTATGTGTGTGTAATGTGTATAATGGTAGAGTGTGGATGGAATATCTTTTGGTATTATATTATTTTGAAGTAGGAGTCAAAATTAGGATATTAGGGGAGTTGGGGAGTGTTGAAAGTCCACAAAGTAATAGTGTTTAGTGTGCTGAATAGAAGGCGACAAGTTCACAGTTCTCACGTGTGCACTCAAATTGTATTCTATGGGTTTTATTATTATTATTCTTTTGATGATGAAGGGAAAATAATAATAATAATACAAAAGCCATTCTATTTCTAATTCCCTATTGATTGGGCACAGGAATTCCTCCTGCTCTGCCCATGTGGCCCAATCTGATATGGACATCTCTTGTTTATTCAACTCTATTTTCTTTCTTTTCCCTTTCCATTCCTACATTATTCAATATAATAGTGTCCCACCTTTTTATTCCTCTCCCTGCTCATCTGCTTCAAATCTGTTGATGTGATTTTTCCAACCTTGGGTTTGTTTGTATTAATTTGAAGCGTAAATTATTTTAATTTAAATAAAATAGAGAGAGATATAGTAGAAAATTGTGTACACTAATTTAAAAAAATAATACTAATTTGATTACTTATAATATAGTGGGAGTTTCATATATGGAGTTAGTTATGGATTCCACCCAACGCTAGTCCAAATAAGTCTTCTAGTTTTTCCCTATCTCACTTCTCTCTAATCTCTACCTACCAATCCGGCTCGGGGAAACGCTTCGGAAGCTTTTGAGGCATAGAAAGGAAGAAAAAGAGGGAAGATTTTTTTTTACTAGATATGGCAAAATGAAAAGAAAATTGTTGACAACCACATTTCCTCACTTTATGGTGATTGATTGAAGTTGAGCTGTTGAAAGAGAGAGAGAGAGAATTGGGTTTCTTTTTCTATTTTTTTAATGTATAAAATCGATGCATTTTTAGATAGAGACACGTGTGTTCTAAGCAATAGGGGAGTAAAAAATTTCCGATGTCTTGGTACACACGCGTGTAACCTCCTCCCTAATCTCCATCTATAGACCTTACATAATTAGACATTTTCTAGACCTTTAAACAATTATTAATTTATGTGTTGACTTCATAATCAATTAATGTGATCACTAACAGTAGTGTGAAATATAGGGGATTTATTCTTATCACGACACAGTAAACAATTTCACTCAGCAGTGGAGATGAAAGATAGAATCATATTCTAATGAAATGTTAGCTTAATTATCATTAAGCTAAACTTATTTTAATTATGATTTTTAATGCGTAGGGAGTGCCAGATTTTTCTGAATATGGAGATGTTTACAGCCTAGGATTTGGACTAGGATCCGAACAACCTCCCACCACTGCGGTCATCAAGAGGGAATTCCAACGTAAAATCGCCTATCACATGAGTCCCCAAGAGGTACCTAAACCCATTCTATAAAACGAATGAATGAATTAAAGGACGTAGTGTTTGATGTTATTAATTAAATCTATGTTAGGATTCGACACTAGCAGCGATGCTACTGAGACCAGGGCCAATCCAGGCGTTAATGTCTGCAGAGTTCAGAGATGAGGAGCGCAGCAGCAGCGAGGAGGAGGGCGCAGAGAAGGTGAAACGAGTGTATATAAGAACAATGTACGATCGAGTAATAAAACCGGAGCAACAAGAAGCCATGATAAAGAAATGGCCACCAGAAATCGTATACGAAATGGATACCGATCACAGCCCTTTTTTCTCCAATCCATCTCTACTCTTTGGCTTGCTTGTAAAAAGCTCTACCCTAAATATGGGGGGATATGTAATTAATTAATTAATTAACTAAACATTTTTTTTCTTTTTAAAATTTGATCTTTTCTTCCTACTAAAGTATTATTATTATTATAAGAACCAAC

mRNA sequence

ACTTCAACTACACAGACTCTATATATATAATACACATTTACAATCATTACTTCCAATTCGTAAATATTTGAAGAACCGCCATGGGAGAGAAAGAAAACCTGGCGCCAAGTTCAGTGAACTCTGTTTCAGTGCAGATTCCGCCGCACTTCGTTCTCGTTCATGGCATAAGTGGAGGAGGTTGGTGCTGGTACAAAATCAGATGTTTAATGGAGAATTCTGGCTTTAAAGTCACTTGTATTGATCTCAAAGGCGCTGGAATCGATCGCTCTGATCCGAATTCCGTCTTCAATTTCGACGATTACAATCAGCCTCTGCTCGATTTCATTTCTACTTTGCCGGAAAACGAACAGATCATTCTGGTTGGACATAGCGCTGGAGGATTAAGTGTTACTCAAGCGACGCTTAAGTTCGCGAAGAAAATCCGTTTGGCGGTGTATGTAGCGGCAACGATGCTGAGATTTGGATTCCAGAATGATCAAGATATTAAAGACGGAGTGCCAGATTTTTCTGAATATGGAGATGTTTACAGCCTAGGATTTGGACTAGGATCCGAACAACCTCCCACCACTGCGGTCATCAAGAGGGAATTCCAACGTAAAATCGCCTATCACATGAGTCCCCAAGAGGATTCGACACTAGCAGCGATGCTACTGAGACCAGGGCCAATCCAGGCGTTAATGTCTGCAGAGTTCAGAGATGAGGAGCGCAGCAGCAGCGAGGAGGAGGGCGCAGAGAAGGTGAAACGAGTGTATATAAGAACAATGTACGATCGAGTAATAAAACCGGAGCAACAAGAAGCCATGATAAAGAAATGGCCACCAGAAATCGTATACGAAATGGATACCGATCACAGCCCTTTTTTCTCCAATCCATCTCTACTCTTTGGCTTGCTTGTAAAAAGCTCTACCCTAAATATGGGGGGATATGTAATTAATTAATTAATTAACTAAACATTTTTTTTCTTTTTAAAATTTGATCTTTTCTTCCTACTAAAGTATTATTATTATTATAAGAACCAAC

Coding sequence (CDS)

ATGGGAGAGAAAGAAAACCTGGCGCCAAGTTCAGTGAACTCTGTTTCAGTGCAGATTCCGCCGCACTTCGTTCTCGTTCATGGCATAAGTGGAGGAGGTTGGTGCTGGTACAAAATCAGATGTTTAATGGAGAATTCTGGCTTTAAAGTCACTTGTATTGATCTCAAAGGCGCTGGAATCGATCGCTCTGATCCGAATTCCGTCTTCAATTTCGACGATTACAATCAGCCTCTGCTCGATTTCATTTCTACTTTGCCGGAAAACGAACAGATCATTCTGGTTGGACATAGCGCTGGAGGATTAAGTGTTACTCAAGCGACGCTTAAGTTCGCGAAGAAAATCCGTTTGGCGGTGTATGTAGCGGCAACGATGCTGAGATTTGGATTCCAGAATGATCAAGATATTAAAGACGGAGTGCCAGATTTTTCTGAATATGGAGATGTTTACAGCCTAGGATTTGGACTAGGATCCGAACAACCTCCCACCACTGCGGTCATCAAGAGGGAATTCCAACGTAAAATCGCCTATCACATGAGTCCCCAAGAGGATTCGACACTAGCAGCGATGCTACTGAGACCAGGGCCAATCCAGGCGTTAATGTCTGCAGAGTTCAGAGATGAGGAGCGCAGCAGCAGCGAGGAGGAGGGCGCAGAGAAGGTGAAACGAGTGTATATAAGAACAATGTACGATCGAGTAATAAAACCGGAGCAACAAGAAGCCATGATAAAGAAATGGCCACCAGAAATCGTATACGAAATGGATACCGATCACAGCCCTTTTTTCTCCAATCCATCTCTACTCTTTGGCTTGCTTGTAAAAAGCTCTACCCTAAATATGGGGGGATATGTAATTAATTAA

Protein sequence

MGEKENLAPSSVNSVSVQIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSSTLNMGGYVIN*
Homology
BLAST of CSPI05G30180 vs. ExPASy Swiss-Prot
Match: Q9SG92 (Methylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=MES17 PE=1 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 6.5e-98
Identity = 162/256 (63.28%), Postives = 211/256 (82.42%), Query Frame = 0

Query: 20  PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLL 79
           PPHFVL+HG+S G WCWYKI+CLME SGF VTCIDLK +GID S  +S+  FD YNQPL+
Sbjct: 18  PPHFVLIHGMSLGSWCWYKIKCLMEVSGFTVTCIDLKSSGIDSSSVDSLTTFDQYNQPLI 77

Query: 80  DFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQNDQDIKDGV 139
           DF+S+ PE EQ+ILVGHSAGGLS+T A  +F KKI LAV++ A+ML+ G Q D+D+KDGV
Sbjct: 78  DFLSSFPEQEQVILVGHSAGGLSLTSAIQRFPKKICLAVFIGASMLKNGLQTDEDMKDGV 137

Query: 140 PDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQAL 199
           PD SE+GDVY LGFGLG E PPT+A+IK E++RK+ YHMSPQ++ +LAA+++RP PI AL
Sbjct: 138 PDLSEHGDVYELGFGLGPENPPTSAIIKPEYRRKLLYHMSPQQECSLAALMMRPAPILAL 197

Query: 200 MSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEMDTDHSP 259
            +A+  +EE+   +E   E+V RVYI+T+ DRV+KPEQQ+AMI++WPP  VYE+++DHSP
Sbjct: 198 TTAKLEEEEKEKGQE---EQVPRVYIKTLLDRVMKPEQQDAMIRRWPPSQVYELESDHSP 257

Query: 260 FFSNPSLLFGLLVKSS 276
           FFSNP +LFGLL+K++
Sbjct: 258 FFSNPFVLFGLLIKAA 270

BLAST of CSPI05G30180 vs. ExPASy Swiss-Prot
Match: Q2V0W1 (Pheophorbidase OS=Raphanus sativus OX=3726 GN=PPD PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.9e-57
Identity = 114/254 (44.88%), Postives = 163/254 (64.17%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDF 81
           HFV VHG S G WCWYK+  L+  +GFK T +DL GAGI+ +D N+VF+FD YN+PL   
Sbjct: 13  HFVFVHGASHGAWCWYKLTTLLVAAGFKATSVDLTGAGINLTDSNTVFDFDHYNRPLFSL 72

Query: 82  ISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQNDQDIKDGVPD 141
           +S LP + +I+LVGHS GG SVT+A  KF  KI + VY+AA M++ G  +     D +  
Sbjct: 73  LSDLPSHHKIVLVGHSIGGGSVTEALCKFTDKISMVVYLAADMVQPG--STSSTHDSIMT 132

Query: 142 FSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQALMS 201
             E  D++   +G G+++PPT  ++K EF+R   Y  SP ED +LA+ LLRP P++AL  
Sbjct: 133 VGE-EDIWEYIYGEGADKPPTGVLMKEEFRRHYYYSQSPLEDVSLASKLLRPAPVRALGG 192

Query: 202 AEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTDHSPF 261
           A+        S    AEKV RVYI+T  D +  P +Q+ +++KWPP  +Y + ++DHS F
Sbjct: 193 AD------KLSPNPEAEKVPRVYIKTAKDNLFDPLRQDRLVEKWPPSQLYILEESDHSAF 252

Query: 262 FSNPSLLFGLLVKS 275
           FS P+ LF  L+++
Sbjct: 253 FSVPTTLFAYLLRA 257

BLAST of CSPI05G30180 vs. ExPASy Swiss-Prot
Match: O23512 (Probable pheophorbidase OS=Arabidopsis thaliana OX=3702 GN=PPD PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 9.9e-54
Identity = 114/257 (44.36%), Postives = 161/257 (62.65%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDF 81
           HFV VHG S G WCWYK+  L++ +GFK T +DL GAGI   D N VF+ D YN+PL   
Sbjct: 12  HFVFVHGASHGAWCWYKLTTLLDAAGFKSTSVDLTGAGISLIDSNIVFDSDQYNRPLFSL 71

Query: 82  ISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFG---FQNDQDIKDG 141
           +S LP + ++ILVGHS GG SVT+A  KF  KI +A+Y+AA+M++ G     +  +I  G
Sbjct: 72  LSDLPPHHKVILVGHSIGGGSVTEALCKFTDKISMAIYLAASMVQPGSIPSPHLSNIHVG 131

Query: 142 VPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQA 201
             D  EY       +G G+++PPT  ++K EF R   Y  SP ED TL++ LLRP P++A
Sbjct: 132 EEDIWEY------TYGEGTDKPPTGVLMKPEFIRHYYYSQSPLEDVTLSSKLLRPAPMRA 191

Query: 202 LMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTDH 261
                F+D ++     E AEKV RVYI+T  D +    +Q+ +++ WPP  +Y + D+DH
Sbjct: 192 -----FQDLDKLPPNPE-AEKVPRVYIKTAKDNLFDSVRQDLLVENWPPSQLYVLEDSDH 251

Query: 262 SPFFSNPSLLFGLLVKS 275
           S FFS P+ LF  L+++
Sbjct: 252 SAFFSVPTTLFAYLLRA 256

BLAST of CSPI05G30180 vs. ExPASy Swiss-Prot
Match: Q9LVL9 (Methylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=MES18 PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 5.4e-52
Identity = 108/262 (41.22%), Postives = 156/262 (59.54%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDF 81
           HFV VHG   GGWCWYK+   + ++G K TCIDLKGAGI+ +DPN+V + DDY++PL  F
Sbjct: 5   HFVFVHGAGHGGWCWYKLANSLRDNGHKATCIDLKGAGINPTDPNTVSSLDDYDEPLYAF 64

Query: 82  ISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFG------FQNDQDI 141
           +S LP ++++ILV HS GG S+T A   F  K+ LAVYVAA M++ G       +N   I
Sbjct: 65  LSQLPNDQKVILVSHSVGGGSMTAAMCLFPSKVSLAVYVAAAMVKPGTLIPERLKNVMKI 124

Query: 142 KDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGP 201
             G+ +  E   ++   FG G +  PT+ ++K E+ R   Y+ SP ED TLA  LLRP P
Sbjct: 125 CSGLIE-EETEKIWDFTFGNGPQNLPTSIMMKPEYVRDKFYNESPMEDYTLATTLLRPAP 184

Query: 202 IQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-D 261
           + A +                 +K+ RVY++T  D + +P  QE M+  WPP   + + D
Sbjct: 185 VMAFIGI------MDIPGAPETDKIPRVYVKTGKDHLFEPVLQEVMLALWPPAHTFLLPD 244

Query: 262 TDHSPFFSNPSLLFGLLVKSST 277
           +DHS FFS P  L+  L+++++
Sbjct: 245 SDHSAFFSQPQELYQFLLQAAS 259

BLAST of CSPI05G30180 vs. ExPASy Swiss-Prot
Match: Q9FVW3 (Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES14 PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.1e-41
Identity = 88/253 (34.78%), Postives = 147/253 (58.10%), Query Frame = 0

Query: 23  FVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDFI 82
           FVLVHG   G WCWYK+   +E SG     +DL G G + +D N+V   ++Y++PL+D +
Sbjct: 98  FVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLL 157

Query: 83  STLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQNDQDIKDGVPDF 142
             LPE E++ILVGHS GG S++ A  +F +KI  A++V ATM+  G +      + +   
Sbjct: 158 ENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATMVSDGQRPFDVFSEELGSA 217

Query: 143 SEY-GDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQALMS 202
             +  +   L +G G ++PPT  + ++   + + ++ SP +D  LA + +RP P+  +M 
Sbjct: 218 ERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPNKDIALAMISMRPVPLGPMM- 277

Query: 203 AEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTDHSPF 262
                 E+ S   E   K +R Y++T+ DR + P+ QE ++++  PE V+++  +DH PF
Sbjct: 278 ------EKVSLTAERYGKGRRFYVQTLDDRALSPDVQEKLVRENSPEGVFKIKGSDHCPF 337

Query: 263 FSNPSLLFGLLVK 274
           FS P  L  +L++
Sbjct: 338 FSKPQSLHKILLE 343

BLAST of CSPI05G30180 vs. ExPASy TrEMBL
Match: A0A5A7TBF5 (Methylesterase 17 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001670 PE=4 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 5.5e-140
Identity = 246/278 (88.49%), Postives = 260/278 (93.53%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVQIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSSVNSVS+  PPHFVLVHGI GGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVNSVSLHNPPHFVLVHGIGGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           D SDPNSV NFDDYNQPLLDFIS+LPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVINFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQ DQDIKDGVPD SEYGD+Y+L FGLG +QPPTTAVIKREFQRK+AYHMSP
Sbjct: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYNLEFGLGPQQPPTTAVIKREFQRKVAYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEA 240
            EDSTLAAMLLRPGPIQALMSA+F++EE    + E A+KVKRVYIRTMYDRVIKPEQQEA
Sbjct: 181 HEDSTLAAMLLRPGPIQALMSAQFKEEE----DREEADKVKRVYIRTMYDRVIKPEQQEA 240

Query: 241 MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSSTLN 279
           MIK WPPEIVYEMDTDHSPFFSNPSLLFGLL+KS +LN
Sbjct: 241 MIKGWPPEIVYEMDTDHSPFFSNPSLLFGLLIKSCSLN 274

BLAST of CSPI05G30180 vs. ExPASy TrEMBL
Match: A0A1S3CAT2 (methylesterase 17 OS=Cucumis melo OX=3656 GN=LOC103498605 PE=4 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 5.5e-140
Identity = 246/278 (88.49%), Postives = 260/278 (93.53%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVQIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSSVNSVS+  PPHFVLVHGI GGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVNSVSLHNPPHFVLVHGIGGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           D SDPNSV NFDDYNQPLLDFIS+LPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVINFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQ DQDIKDGVPD SEYGD+Y+L FGLG +QPPTTAVIKREFQRK+AYHMSP
Sbjct: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYNLEFGLGPQQPPTTAVIKREFQRKVAYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEA 240
            EDSTLAAMLLRPGPIQALMSA+F++EE    + E A+KVKRVYIRTMYDRVIKPEQQEA
Sbjct: 181 HEDSTLAAMLLRPGPIQALMSAQFKEEE----DREEADKVKRVYIRTMYDRVIKPEQQEA 240

Query: 241 MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSSTLN 279
           MIK WPPEIVYEMDTDHSPFFSNPSLLFGLL+KS +LN
Sbjct: 241 MIKGWPPEIVYEMDTDHSPFFSNPSLLFGLLIKSCSLN 274

BLAST of CSPI05G30180 vs. ExPASy TrEMBL
Match: A0A6J1JV68 (methylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111489169 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.3e-129
Identity = 230/275 (83.64%), Postives = 247/275 (89.82%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVQIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV+VQ PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           D SDPNSV  FDDYN+PLLDF+S+LPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQ DQDIK+GVPD SE+GDVY +GFGLG +QPPTTAVIKRE QRKI YHMSP
Sbjct: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDVYDVGFGLGPQQPPTTAVIKRELQRKIIYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEA 240
            EDSTLA+MLLRPGPI AL +A FR+E          + VKRVYIRT YDRVIKPEQQEA
Sbjct: 181 HEDSTLASMLLRPGPILALRTARFREENGE------LDTVKRVYIRTTYDRVIKPEQQEA 240

Query: 241 MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSS 276
           MI++W PEIVYEMDTDHSPFFSNPSLLFGLLVK++
Sbjct: 241 MIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAA 269

BLAST of CSPI05G30180 vs. ExPASy TrEMBL
Match: A0A6J1BYB8 (methylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111006571 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.2e-128
Identity = 231/279 (82.80%), Postives = 253/279 (90.68%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVQIP----PHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLK 60
           MGE++NLAPS+V SV+VQ P    PHFVLVHGISGGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEEDNLAPSAV-SVAVQKPLPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120
           GAGIDRSDPNSV  FDDYN+PLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL
Sbjct: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120

Query: 121 AVYVAATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAY 180
           AVYVAATML+ G+Q DQDIKDGVPD SE+GDVY L FGLGS+QPPTTA+IKREFQRKI Y
Sbjct: 121 AVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICY 180

Query: 181 HMSPQEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPE 240
           HMSP EDSTL AMLLRPGPI AL +A+FR+      EEE A+KVKRV+IRTMYDRVIK E
Sbjct: 181 HMSPHEDSTLGAMLLRPGPILALKTAQFRE------EEEFADKVKRVFIRTMYDRVIKRE 240

Query: 241 QQEAMIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSS 276
           QQ+AMIK+WPPEIVY+MDTDHSPFFSNPSLLFGLL+K++
Sbjct: 241 QQDAMIKRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAA 272

BLAST of CSPI05G30180 vs. ExPASy TrEMBL
Match: A0A6J1FL47 (methylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111444979 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 3.7e-128
Identity = 227/275 (82.55%), Postives = 246/275 (89.45%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVQIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV+VQ PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           D SDPNSV  FDDYN+PLLDF+S+LPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQ DQDIK+GVPD SE+GD Y +GFG G +QPPTTAV+KRE QRKI YHMSP
Sbjct: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEA 240
            EDSTLA+MLLRPGPI AL +A FR+E          ++VKRVYIRT YDRVIKPEQQEA
Sbjct: 181 HEDSTLASMLLRPGPILALRTARFREENGE------LDRVKRVYIRTTYDRVIKPEQQEA 240

Query: 241 MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSS 276
           MI++W PEIVYEMDTDHSPFFSNPSLLFGLLVK++
Sbjct: 241 MIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAA 269

BLAST of CSPI05G30180 vs. NCBI nr
Match: XP_004141511.1 (methylesterase 17 [Cucumis sativus] >KAE8648969.1 hypothetical protein Csa_008717 [Cucumis sativus])

HSP 1 Score: 580.9 bits (1496), Expect = 6.2e-162
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVQIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSSVNSVSVQIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVNSVSVQIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP
Sbjct: 121 AATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEA 240
           QEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEA
Sbjct: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEA 240

Query: 241 MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSSTLNMGGYVIN 286
           MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSSTLNMGGYVIN
Sbjct: 241 MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSSTLNMGGYVIN 285

BLAST of CSPI05G30180 vs. NCBI nr
Match: XP_008459483.1 (PREDICTED: methylesterase 17 [Cucumis melo] >KAA0039366.1 methylesterase 17 [Cucumis melo var. makuwa])

HSP 1 Score: 506.9 bits (1304), Expect = 1.1e-139
Identity = 246/278 (88.49%), Postives = 260/278 (93.53%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVQIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSSVNSVS+  PPHFVLVHGI GGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVNSVSLHNPPHFVLVHGIGGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           D SDPNSV NFDDYNQPLLDFIS+LPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVINFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQ DQDIKDGVPD SEYGD+Y+L FGLG +QPPTTAVIKREFQRK+AYHMSP
Sbjct: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYNLEFGLGPQQPPTTAVIKREFQRKVAYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEA 240
            EDSTLAAMLLRPGPIQALMSA+F++EE    + E A+KVKRVYIRTMYDRVIKPEQQEA
Sbjct: 181 HEDSTLAAMLLRPGPIQALMSAQFKEEE----DREEADKVKRVYIRTMYDRVIKPEQQEA 240

Query: 241 MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSSTLN 279
           MIK WPPEIVYEMDTDHSPFFSNPSLLFGLL+KS +LN
Sbjct: 241 MIKGWPPEIVYEMDTDHSPFFSNPSLLFGLLIKSCSLN 274

BLAST of CSPI05G30180 vs. NCBI nr
Match: XP_038890470.1 (methylesterase 17 [Benincasa hispida])

HSP 1 Score: 493.0 bits (1268), Expect = 1.7e-135
Identity = 240/280 (85.71%), Postives = 258/280 (92.14%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVQIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSSV+SVSVQ PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVDSVSVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DRSDPNSV  FDDYN+PLLDFIS+LPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DRSDPNSVLKFDDYNKPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQ D+D KDGVPD SE+GDVY + FGLG +QPPTTAVIKREFQRKI YHMSP
Sbjct: 121 AATMLRFGFQTDRDFKDGVPDLSEFGDVYDVEFGLGPQQPPTTAVIKREFQRKIIYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEA 240
            EDSTLAAMLLRPGPI AL +A+F +      E++GA+KVKRVYIRTMYDRVIKPEQQ+A
Sbjct: 181 HEDSTLAAMLLRPGPILALKTAQFME------EQDGADKVKRVYIRTMYDRVIKPEQQDA 240

Query: 241 MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSSTLNMG 281
           MIK WPPEIVYEMDTDHSPFFS+PSLLFGLLVKS +L+MG
Sbjct: 241 MIKGWPPEIVYEMDTDHSPFFSDPSLLFGLLVKSCSLSMG 274

BLAST of CSPI05G30180 vs. NCBI nr
Match: XP_022993026.1 (methylesterase 17 [Cucurbita maxima])

HSP 1 Score: 470.7 bits (1210), Expect = 9.0e-129
Identity = 230/275 (83.64%), Postives = 247/275 (89.82%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVQIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV+VQ PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           D SDPNSV  FDDYN+PLLDF+S+LPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQ DQDIK+GVPD SE+GDVY +GFGLG +QPPTTAVIKRE QRKI YHMSP
Sbjct: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDVYDVGFGLGPQQPPTTAVIKRELQRKIIYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEA 240
            EDSTLA+MLLRPGPI AL +A FR+E          + VKRVYIRT YDRVIKPEQQEA
Sbjct: 181 HEDSTLASMLLRPGPILALRTARFREENGE------LDTVKRVYIRTTYDRVIKPEQQEA 240

Query: 241 MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSS 276
           MI++W PEIVYEMDTDHSPFFSNPSLLFGLLVK++
Sbjct: 241 MIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAA 269

BLAST of CSPI05G30180 vs. NCBI nr
Match: XP_022134269.1 (methylesterase 17 [Momordica charantia])

HSP 1 Score: 468.4 bits (1204), Expect = 4.5e-128
Identity = 231/279 (82.80%), Postives = 253/279 (90.68%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVQIP----PHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLK 60
           MGE++NLAPS+V SV+VQ P    PHFVLVHGISGGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEEDNLAPSAV-SVAVQKPLPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120
           GAGIDRSDPNSV  FDDYN+PLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL
Sbjct: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120

Query: 121 AVYVAATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAY 180
           AVYVAATML+ G+Q DQDIKDGVPD SE+GDVY L FGLGS+QPPTTA+IKREFQRKI Y
Sbjct: 121 AVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICY 180

Query: 181 HMSPQEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPE 240
           HMSP EDSTL AMLLRPGPI AL +A+FR+      EEE A+KVKRV+IRTMYDRVIK E
Sbjct: 181 HMSPHEDSTLGAMLLRPGPILALKTAQFRE------EEEFADKVKRVFIRTMYDRVIKRE 240

Query: 241 QQEAMIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSS 276
           QQ+AMIK+WPPEIVY+MDTDHSPFFSNPSLLFGLL+K++
Sbjct: 241 QQDAMIKRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAA 272

BLAST of CSPI05G30180 vs. TAIR 10
Match: AT3G10870.1 (methyl esterase 17 )

HSP 1 Score: 358.6 bits (919), Expect = 4.6e-99
Identity = 162/256 (63.28%), Postives = 211/256 (82.42%), Query Frame = 0

Query: 20  PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLL 79
           PPHFVL+HG+S G WCWYKI+CLME SGF VTCIDLK +GID S  +S+  FD YNQPL+
Sbjct: 18  PPHFVLIHGMSLGSWCWYKIKCLMEVSGFTVTCIDLKSSGIDSSSVDSLTTFDQYNQPLI 77

Query: 80  DFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQNDQDIKDGV 139
           DF+S+ PE EQ+ILVGHSAGGLS+T A  +F KKI LAV++ A+ML+ G Q D+D+KDGV
Sbjct: 78  DFLSSFPEQEQVILVGHSAGGLSLTSAIQRFPKKICLAVFIGASMLKNGLQTDEDMKDGV 137

Query: 140 PDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQAL 199
           PD SE+GDVY LGFGLG E PPT+A+IK E++RK+ YHMSPQ++ +LAA+++RP PI AL
Sbjct: 138 PDLSEHGDVYELGFGLGPENPPTSAIIKPEYRRKLLYHMSPQQECSLAALMMRPAPILAL 197

Query: 200 MSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEMDTDHSP 259
            +A+  +EE+   +E   E+V RVYI+T+ DRV+KPEQQ+AMI++WPP  VYE+++DHSP
Sbjct: 198 TTAKLEEEEKEKGQE---EQVPRVYIKTLLDRVMKPEQQDAMIRRWPPSQVYELESDHSP 257

Query: 260 FFSNPSLLFGLLVKSS 276
           FFSNP +LFGLL+K++
Sbjct: 258 FFSNPFVLFGLLIKAA 270

BLAST of CSPI05G30180 vs. TAIR 10
Match: AT4G16690.1 (methyl esterase 16 )

HSP 1 Score: 211.8 bits (538), Expect = 7.0e-55
Identity = 114/257 (44.36%), Postives = 161/257 (62.65%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDF 81
           HFV VHG S G WCWYK+  L++ +GFK T +DL GAGI   D N VF+ D YN+PL   
Sbjct: 12  HFVFVHGASHGAWCWYKLTTLLDAAGFKSTSVDLTGAGISLIDSNIVFDSDQYNRPLFSL 71

Query: 82  ISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFG---FQNDQDIKDG 141
           +S LP + ++ILVGHS GG SVT+A  KF  KI +A+Y+AA+M++ G     +  +I  G
Sbjct: 72  LSDLPPHHKVILVGHSIGGGSVTEALCKFTDKISMAIYLAASMVQPGSIPSPHLSNIHVG 131

Query: 142 VPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQA 201
             D  EY       +G G+++PPT  ++K EF R   Y  SP ED TL++ LLRP P++A
Sbjct: 132 EEDIWEY------TYGEGTDKPPTGVLMKPEFIRHYYYSQSPLEDVTLSSKLLRPAPMRA 191

Query: 202 LMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTDH 261
                F+D ++     E AEKV RVYI+T  D +    +Q+ +++ WPP  +Y + D+DH
Sbjct: 192 -----FQDLDKLPPNPE-AEKVPRVYIKTAKDNLFDSVRQDLLVENWPPSQLYVLEDSDH 251

Query: 262 SPFFSNPSLLFGLLVKS 275
           S FFS P+ LF  L+++
Sbjct: 252 SAFFSVPTTLFAYLLRA 256

BLAST of CSPI05G30180 vs. TAIR 10
Match: AT5G58310.1 (methyl esterase 18 )

HSP 1 Score: 206.1 bits (523), Expect = 3.8e-53
Identity = 108/262 (41.22%), Postives = 156/262 (59.54%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDF 81
           HFV VHG   GGWCWYK+   + ++G K TCIDLKGAGI+ +DPN+V + DDY++PL  F
Sbjct: 5   HFVFVHGAGHGGWCWYKLANSLRDNGHKATCIDLKGAGINPTDPNTVSSLDDYDEPLYAF 64

Query: 82  ISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFG------FQNDQDI 141
           +S LP ++++ILV HS GG S+T A   F  K+ LAVYVAA M++ G       +N   I
Sbjct: 65  LSQLPNDQKVILVSHSVGGGSMTAAMCLFPSKVSLAVYVAAAMVKPGTLIPERLKNVMKI 124

Query: 142 KDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGP 201
             G+ +  E   ++   FG G +  PT+ ++K E+ R   Y+ SP ED TLA  LLRP P
Sbjct: 125 CSGLIE-EETEKIWDFTFGNGPQNLPTSIMMKPEYVRDKFYNESPMEDYTLATTLLRPAP 184

Query: 202 IQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-D 261
           + A +                 +K+ RVY++T  D + +P  QE M+  WPP   + + D
Sbjct: 185 VMAFIGI------MDIPGAPETDKIPRVYVKTGKDHLFEPVLQEVMLALWPPAHTFLLPD 244

Query: 262 TDHSPFFSNPSLLFGLLVKSST 277
           +DHS FFS P  L+  L+++++
Sbjct: 245 SDHSAFFSQPQELYQFLLQAAS 259

BLAST of CSPI05G30180 vs. TAIR 10
Match: AT1G33990.1 (methyl esterase 14 )

HSP 1 Score: 171.8 bits (434), Expect = 8.0e-43
Identity = 88/253 (34.78%), Postives = 147/253 (58.10%), Query Frame = 0

Query: 23  FVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDFI 82
           FVLVHG   G WCWYK+   +E SG     +DL G G + +D N+V   ++Y++PL+D +
Sbjct: 98  FVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLL 157

Query: 83  STLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQNDQDIKDGVPDF 142
             LPE E++ILVGHS GG S++ A  +F +KI  A++V ATM+  G +      + +   
Sbjct: 158 ENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATMVSDGQRPFDVFSEELGSA 217

Query: 143 SEY-GDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQALMS 202
             +  +   L +G G ++PPT  + ++   + + ++ SP +D  LA + +RP P+  +M 
Sbjct: 218 ERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPNKDIALAMISMRPVPLGPMM- 277

Query: 203 AEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTDHSPF 262
                 E+ S   E   K +R Y++T+ DR + P+ QE ++++  PE V+++  +DH PF
Sbjct: 278 ------EKVSLTAERYGKGRRFYVQTLDDRALSPDVQEKLVRENSPEGVFKIKGSDHCPF 337

Query: 263 FSNPSLLFGLLVK 274
           FS P  L  +L++
Sbjct: 338 FSKPQSLHKILLE 343

BLAST of CSPI05G30180 vs. TAIR 10
Match: AT3G29770.1 (methyl esterase 11 )

HSP 1 Score: 169.5 bits (428), Expect = 4.0e-42
Identity = 98/261 (37.55%), Postives = 140/261 (53.64%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDF 81
           HFVLVHG S G WCWYK   L+E  GFKVT IDL G GI+  + N + +   Y +PL D 
Sbjct: 138 HFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSININGIASLSQYVKPLTDI 197

Query: 82  ISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQNDQD---IKDG 141
           +  LP  E++ILVGH  GG  ++ A   F  KI  AV++AA ML  G Q+  D   +K G
Sbjct: 198 LEKLPIGEKVILVGHDFGGACISYAMELFPSKISKAVFLAAAMLTNG-QSTLDMFSLKAG 257

Query: 142 VPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQA 201
             D      ++   +  G+E PPT   + +   + + ++ SP +D  LA++ +R  P   
Sbjct: 258 QNDLMRKAQIFI--YTNGNENPPTAIDLDKSLLKDLLFNQSPSKDVALASVSMRSIPFAP 317

Query: 202 LMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTDH 261
           ++       E+ S  +     V+R YI T+ D  I    QE MI   PPE VY +   DH
Sbjct: 318 VL-------EKLSLSDANYGSVRRYYIETLEDNAIPVTLQENMINSSPPEKVYRLKGADH 377

Query: 262 SPFFSNPSLLFGLLVKSSTLN 279
           +PFFS P  L  LL++ + ++
Sbjct: 378 APFFSKPQALHKLLLEIARIS 388

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SG926.5e-9863.28Methylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=MES17 PE=1 SV=1[more]
Q2V0W11.9e-5744.88Pheophorbidase OS=Raphanus sativus OX=3726 GN=PPD PE=1 SV=1[more]
O235129.9e-5444.36Probable pheophorbidase OS=Arabidopsis thaliana OX=3702 GN=PPD PE=1 SV=1[more]
Q9LVL95.4e-5241.22Methylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=MES18 PE=2 SV=1[more]
Q9FVW31.1e-4134.78Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Match NameE-valueIdentityDescription
A0A5A7TBF55.5e-14088.49Methylesterase 17 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001... [more]
A0A1S3CAT25.5e-14088.49methylesterase 17 OS=Cucumis melo OX=3656 GN=LOC103498605 PE=4 SV=1[more]
A0A6J1JV684.3e-12983.64methylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111489169 PE=4 SV=1[more]
A0A6J1BYB82.2e-12882.80methylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111006571 PE=4 SV=1[more]
A0A6J1FL473.7e-12882.55methylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111444979 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004141511.16.2e-162100.00methylesterase 17 [Cucumis sativus] >KAE8648969.1 hypothetical protein Csa_00871... [more]
XP_008459483.11.1e-13988.49PREDICTED: methylesterase 17 [Cucumis melo] >KAA0039366.1 methylesterase 17 [Cuc... [more]
XP_038890470.11.7e-13585.71methylesterase 17 [Benincasa hispida][more]
XP_022993026.19.0e-12983.64methylesterase 17 [Cucurbita maxima][more]
XP_022134269.14.5e-12882.80methylesterase 17 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G10870.14.6e-9963.28methyl esterase 17 [more]
AT4G16690.17.0e-5544.36methyl esterase 16 [more]
AT5G58310.13.8e-5341.22methyl esterase 18 [more]
AT1G33990.18.0e-4334.78methyl esterase 14 [more]
AT3G29770.14.0e-4237.55methyl esterase 11 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 21..263
e-value: 7.2E-15
score: 55.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 18..275
e-value: 8.6E-57
score: 194.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 21..272
NoneNo IPR availablePANTHERPTHR10992:SF1043METHYLESTERASE 17-LIKEcoord: 21..274
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 21..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G30180.1CSPI05G30180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0033473 indoleacetic acid conjugate metabolic process
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0009696 salicylic acid metabolic process
biological_process GO:0048367 shoot system development
molecular_function GO:0080030 methyl indole-3-acetate esterase activity
molecular_function GO:0080032 methyl jasmonate esterase activity
molecular_function GO:0080031 methyl salicylate esterase activity