CSPI05G29530 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G29530
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptiontRNA (guanine(26)-N(2))-dimethyltransferase
LocationChr5: 27738516 .. 27745879 (-)
RNA-Seq ExpressionCSPI05G29530
SyntenyCSPI05G29530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAGTTTGGCGCAAAAACCTCAGCCTCTAGCCGTGGATTGGAAGATTAGGGTTTAAGCCAAAAAAGATCGATTTTTTATTTTATTTTATCATTCTTCGTGCACAGAATTTAAGGGAGCTTTCGATTGTGAAGCTGCTGCTGCTGCTGCTGCTGAGTGGCAATAGGGTTCCACTGTATTAGAAAATTCTTCAAGTCATTAAGGGGTGGTTACTACTTAGTTGTTGGTTTTATTCAACTGCTTGCTCTGTTTGTTCAACCTTCACGAAGTATTGGGAGCTGAAGATTCGGAGGCATTGAGAGTTTTTAGGGTTGTGGATTTTGCTTTGACCCTTGAAATAAGCCAAGGAGCCGAAGAAGGAAGAATCCCAGAATCTGCTTTTTTTGTTTTCTTTCAAATTTTTCTTCATTTTTTTTTATTGCATGTGAGGATTGCAGTAATGGCGACAGATCTTAGCGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTTATGCACGCGAAAAATGAAGTCTTTTTCAATAAGACCCAGGTGCCTTGTTTATTTGAATAATGGGTTTTTTGGGGGTTTTGGAGTTTAGGCATTGTTCTTAATAAAAAAGTTAATTGGTCAGTGAAGAGTTTCGAAGGATTTGCATATGATTGTTTCTAGCACATGAAGTTACGCGTAAGTTGTTGATAGGGGAAATATTTAAAACCCAACTTAAGCTTCATGGGGAAGAAGATAAAAGTCAGGGCATGTTAGCATATTTAAATGATTGATATTTGATGGAGGAAATAAGATACGTAATTTATTCAGGTCAGCAGAGTATAATGTGGTATGCTAAATTCAAAGATATTTTTCCATGTATTATACACATCATGATGGCAATCTATCATCTTTTTCTCACTGCACTTCTTTTACTATATAATTTGTTTGAAGTCCAAGTAGAGTCCCATCAGTTATTAAGAGTATCTGGGAACTATTACCTACAATTTTTTTAAAAAAAATTCTAATATAATCATAGAATTATTGGTTGTTGGATTTTCATTTTTTTGTGGCTTTTTTGGGTCTATACTGGCTTCAAGTTCTCCTCATGGTCGAATCTTGCCTCTGTAATGCTCAGGTTAACAACAGAGATATATCCATTGCTGTCCTTAGGTCATTTATAGCAAAACGAAAACAGGAGCATGAAGCAAAACTATCAAAAAGATCAAAATCAGGGCAGAAGCTTTCTGAAAATAATGGTTCTGAAACTGCTACGGAAGAAGCATGTAATGAATCTGCAACAAATGATGAAAAGTGCGATACAGATTGTGATGCTCATGAAAAACCATCAAATGAATGTGGAAGTCCAATGTTGTCAGAAGAACAAAAAGAGATCGTGGAGGGAAAAGAACAAAGAGGTCCCAAGCCAATTAGAGTTCTTGAGGTTGTTTTAATTTCATTGGCGTTTTATTATGTGCATGTACAAAGTTAATTCCTAGTTTATCTTCCAATAAAGTCTGAAGTTTATACATTTCAAATTAGGAATACGTTCATGCCTTATTTATTAGCCACCTGTTTGAGTTGTAGAGTCTTATGTTCTATAATCTGTCTTCTCCCCTACAAAGACAACCAGAATTCTGAACTGATACGGCTACTGGGAAGGACCTTTGTAACACCCCCAATTGTAAATAAGTTTTTTTTGAGGGGCAGCGCTCTTTGGGGTGTTAGTTGGTTGCAGTTTATATTTTTTGTTTTCTTGTTTGTTTGGGTTTATTTTATCATTATGTTTTTGTGTAATATACTATGGTGAGTGTGTCACAGAGTGGAGAGTGTACACCTTCTTGAAGTGGTGGTTTGCTTGTTTAATTTTCTTATGATAATATAAGTACGCCTTGTGAGTTAGTATTCTTACAACCTTCTAGGGATGGTCAACATGAAGTTCAGTAACAATTTATTAACAATGGATAGACCAAGAGGCTTGAAGTCTATGAAATTCATGTAGATTTTTCAAATATACAAAGATGAAGTTGTTCTTAATAGTGTGTACATTCCATGTTCATTATGAATGTGTCCTTGTTCTAACATATTAGAATTTAGTTCAATATATTGTCATTGGGTTTTATGACTCCGATGTTATATTGTGCGTCTACTATTTTGTTTGTGTTTTCTTCTCTCTTGGGATGTTTGGTATAAGTTATGGTGATATGTTTATCCATTCACTAACTGACTAATTCCTGTTTGAATTTGAAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGAGTTGGTCAAGTTGTGGCGCTAGACAATGATAAAGGTAACTTTTTAATTATTGGTTCTACCAAAAAGTTCTCTTCTGCCTCAAAAAAAGAAAAAGAAAAAATAAAAACTCTTTAGCAAATATGTTGACTTGGCCATTCTTGAAGAGGGGGAGGTGTAAAGATTTGGTAAGTAACGTGTAAAAATTTGGTAAGTATTGTAAACGACAATCTTAAATAATGGTCAACTTATGGGGGAGGGATATCTTATCTTCTTTTTTCTTTTTGTGGTATAAAACTTTTATTGTCTAAAAGAAAATAAAGAAAGAAAAAAGAATCTTGCAACAAGTTTGAAGTTTATAAGGATTGTCAATGGATAACATCTATGCACTCACTTCGTATGACCACAAAGAGCTCAAAGTGGTTACTGATCATTACATGCTTCACTTCTAGAAGGTTTTAGTAAGTGGAACCAGGAACACCACCTTTGGTTGATACTGTAATATTAAAGGCAGCTTTGCCTTGTACAAGCAGAGGAAGTTTGTGTTGATAATTCTTTCCTCTTTTATCTATATATACTTATGTATTCATCTTCTGGAGACATGTATTAATGGACTGCTAATGGACCCTTTGAAACAGAAGTTATCCATTTTACTAATCTTACAATAAGGTCTTATCTACATTGATTTGCTATTTAGTTGTTCCTTTTCAGTTATTGATAACTGAATCTGTTAATGATAAGTTTTTTCCCTTTAAACAGCATCTGTCGAAGCTTGTCGGAGAAACATCAAGTTTAATGGTTCAGTGGCATGTTCAAAAGTGGAATCACATCTTACTGATGCTCGTGTTTATATGCTCACTCACCCAAAAGAATTTGATATGGTATGCACACAGTAATTCAGATATGTAGGTTATTTGGCTCCTCGGAAATTACATATAGGGAATTAACAATTGAGATGCATTTTCTTTTTCCTTGTTGTAGTTTAGATTTGTAACCATGTGAATAATTATGAATAACCACTTGTTGGCCTTTTGTAATTTCACCATTGAAATTCTAATTGAATGTTTCTCATCAGTAATAACATTAACAATATAATAATAATAATAATAATAATAATAATAATGTACGAGTACTGATGTTAATACGCAATTGAAAGTTTTTAGCAACTGCGTTGTTTCAGGTTGATTTGGATCCTTATGGTTCACCTTCTATTTTCTTGGACTCTGCTGTTCAATCAGTCGTAGATGGTGGCATGCTGATGTGTACAGCAACTGATATGGCAGTGCTTTGTGGGGGAAATGGAGAAGTTTGCTACTCAAAGTAAAGTTTTTTGCAGTCTCCAATTATCATTTTTTTAATGTTCACATAGATTATGTGCCCTAAAAATTGCTCTGATTGATTCAGATATGGCTCCTATCCTTTGAGAGGGAAATATTGCCACGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGGTATACCTATCTCCTGTGTATTTCAAAGGTTTTCTTGATGAGTTATTTTACCACCTTTCTGTTTCTCCTTATTCGTTAAAACTTTATGTACAAGATCATTCTTATCTTAACACACACGCAGGGTCATATAGAGCCTCTTTAACTGAAAGATAACGTACTTCAAAATTTTGGTCTCACTAACGAGCAGAAGTGGCCTCTTATGCATAACTTTTGTTCTAAATTCTTTGTAGAGCCATGCAAATAGATACAAGCGGTATATTGTTCCAGTGCTATCTGTTCAGATGGATTTCTATGTCCGGGTTTTTGTTCGTGTTTATACGTAAGTTGCCTTGGACTTTGAATTTAATGGTTAGCTTAGCTATATTATACTACTTTTTTGATGTGTTATAAATATATGTATAAATATTGTAAAATAATTTGTTGCAGTTCTGCAAGTGCAATGAAAAATACTCCCCTTAAGCTCTCATATGTTTATCAGTGCACTGGTTGTGATTCTTTCCATCTCCAGCAGATTGGCAGAAGCGTCTCCAAGGTACACATTTATGCTGGTTAATTGCATAATTGAGTTTATTTTTACTGTCCCATGTAATTTCTCTAACTGGTTTGAGTTTTTCCTGCTTATTTATACATTCTCTTTCCCCCCTCCTGTTTAAACAACTAGTTTTTATTGATTTGAACGAGATTAAAACAAACATCCCACAAAAATGGAACAGGCAGTCAAAAATGGAGAACACAGAATGCAAATTACTATAAATGGGCCTTAACTTTTGATGATAAGAAATTATTCCAAAGTTCTGAATTATGAGTGCAAAGGGACCTATTAAAATGCACCAACCTGCACACATCTTCCTCGCCTTTATCTAGGCAGACTGAAGGCTTGCTTCTTTTCCCTGTACTAGGATGGATTTCTTATCATCCCTTTTCTTCCCCAATATTTAACACTTTGTTTATCCTTGAAGGAAAGAACAAAATATGTTGCCAATGTTACCTATTGATCCTGTACCACAATATCTACTGATGCTTCTATGCCCTTTATATAATATATTTACTTCTGCTTGGAAGTTAATCCTAGTTTGAAGTAGTAATTTATTTCTTTATAATGTGAATTTGTATGGTTTCTTTCTTGATGTGCCACCTATCATTTTTCTTTCAGAACAACAGCGTCAGGTATTTACCTGGTTTTGGTCCTTCTGTTGCTCAAGAGTGCAGTGATTGTGGGAAGAAATTCAACATGGGTGGGCCTATTTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATGCTAGCAGATGTGAAGTCTATGAAGGACTCTTATCCTGCATATGATCGTATATCTGCTGTATTGACAACAATTTCCGAGGTGCGTTTTATTTCTGGGTCTGGCATTACAGCTCCATATTGAACGAACTATTAGTATGATATTAGGCCATTGGATCATGCTGTTGGTTATTCTTGTCAGGACTACTTTGTAATTTTAGCCATTACCTAATTTCCCTAATAAAAAAAATAAACAAAAAGTTTGAAATTGTAGTCAGTTTGGATAATCAGAAAATTCATTATGATAAATTAGGAACCATAAGATTTTTTTGGCCCTCCCCAAGAACGACTAATAAATAAGGACCTTCGATTGTTGAAAATCAAATGAGGAACCAGGAGATGAAATCCTATAGATGCCTTTAGAAGATTAGGGTAGTTTTGAGGATAATTATAATTTTTGGCCAATCAGAAAATTCTTTTCTCCATAAGAATTGAGAAAGTTATAATGATAAAGTATGTATGTTTGTTACAATATTCAAGATAACTTGGCATATTTCATTCTGGAGTATCTCCAATTCCCTAGTACTTCATTGGCCAGCCTTCCCAAGGGATTTGTCCTGAGATCTTTCCATCTCGACCATAGTGTAAAAAAAGGTTCAATAGCTAATGTGATTGTAGCTCAAAAGTAGTTCTAATTGACATAACTTTCCTCCCTGGTGGTCGAAGGTTCAATCCCCATCCTCATAGTTGTACTAAAAAAACCAAATACATAAGATCGTGATATAGCCTTTAAGAAAAAAGGTTCAATAATTTTGTACTTTCAAAAGAAAAGTGAGTATAGATATATACATATTTCTTTCCACTTCATAAATTGGGCAGTGCATGGAAATAATTCCCAGATTGGTTTATTTTCTAACTTGTGAAGGATTAAGTACAACATGCGAATAGTTCAGTGGCATCCCATGGTAAAGAGTCATGCCAAGTACACTCTTATTCATCTGTTGACTGTCTGTTGTGTGCTGAATGCTGAGTCTTTTTCTTTGCACGTTGCACTTATTGACTTGGATGTATTTATCCTGTGCAGGAGCTGATGGACGTTCCTTTATTTTTGAGTCTACACAACCTCTGTGCAACATTGAAGTGCACATCTCCTTCTGCCGTGAATTTCCGTTCGGCAGTGATTAATGCAGGATATCGCATATCGGGCACTCATGTGAATCCATTAGGTCTGAAATCAGATGCTCCCATGGATGTTATTTGGGATATAATGCGTTGCTGGGTAGGGCATTCTGAATTAAAAATTTCATTATATACTCGATTGAATGACATTATGGGGCTGAACAACCCATTGAGTGGAGTTAATATGTTAGAGTTCATAAGTTTGTGTTTGGAGTGTAGAGTTGTATGATTGACTTTTTCGACAAATGTGAAAGATAAAGTTTCTCGATAAATGTGAAAAGTACGGAAAGATAGAACTACTCACTAAATATGCATATTTCATTAGTATTATTGAAATTGAATTGTTTTCATCAATTTAAGAAGTTAGTGGGTCAAATAACCTCTTTATTTGTACTTTGTATATTATAAGCCTTGTTACCCCACGTGGAACTTTAGAGCTTTGTTAGTCCTTAGTCTTGCTTGTATATCGTGATTTCATCGATCCTTTTTTATGTAGGTAAAAAACCACCCAGTTAAAACTCAACCAGAAGATCAGGCAGGAAGTATCATTCTTGCCAAGGAACCAGTTCTTGAAGTAAGTTCCATTTTGTAGTTCCAACTGTTGATTCAGTTGCAATGTTTTTGTCCTCAAGTTTTCCTCTGTCGAACATATCATTATTTCCAAGAGTTAAATTTTGAGTTACCCTTAAGAAATGTACCAAAAACTGAATAGCCTGTGGCTGATGTACTTTTGCTGATTCTAATATTCTTCTTGGCAGGCTAATTTTGCTAGAGCTGTAGCATCTCTTAGCAAGGCACAAGCAAAGAAAATTGCACGCTTCCTTCCAAATCCTGAAAGACATTGGGGCCCCAAGCTCAGAGCAGGTCGTCAAATCACTAGTAAACACATCTCTCTACTTGGTGCAGAGGCAGTAAATGGAGCTCTCAGCCATACGGATTCCGAAGAGCCTAACGCGAAACGTTCCAAGGTAGAAGAAGAACCAGCTCCCAACCAGTAAAAAAAATTCTTTTATCTTATACTTTCTTTTATGCTTCTAAGTTATGCAGTATTACTTTTTGACCCTCCACTTGAAATATTCTTGTTTGGGAACACTTCTACAGAAGTAGAAATTTTTGTTTAATATTGAGGAAGGCTTTCTTTCAAAATATATATCATTTTTGTTCGTCTTTAGGATGTTCAGATTTTTGTCAAGAG

mRNA sequence

ATAAGTTTGGCGCAAAAACCTCAGCCTCTAGCCGTGGATTGGAAGATTAGGGTTTAAGCCAAAAAAGATCGATTTTTTATTTTATTTTATCATTCTTCGTGCACAGAATTTAAGGGAGCTTTCGATTGTGAAGCTGCTGCTGCTGCTGCTGCTGAGTGGCAATAGGGTTCCACTGTATTAGAAAATTCTTCAAGTCATTAAGGGGTGGTTACTACTTAGTTGTTGGTTTTATTCAACTGCTTGCTCTGTTTGTTCAACCTTCACGAAGTATTGGGAGCTGAAGATTCGGAGGCATTGAGAGTTTTTAGGGTTGTGGATTTTGCTTTGACCCTTGAAATAAGCCAAGGAGCCGAAGAAGGAAGAATCCCAGAATCTGCTTTTTTTGTTTTCTTTCAAATTTTTCTTCATTTTTTTTTATTGCATGTGAGGATTGCAGTAATGGCGACAGATCTTAGCGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTTATGCACGCGAAAAATGAAGTCTTTTTCAATAAGACCCAGGTTAACAACAGAGATATATCCATTGCTGTCCTTAGGTCATTTATAGCAAAACGAAAACAGGAGCATGAAGCAAAACTATCAAAAAGATCAAAATCAGGGCAGAAGCTTTCTGAAAATAATGGTTCTGAAACTGCTACGGAAGAAGCATGTAATGAATCTGCAACAAATGATGAAAAGTGCGATACAGATTGTGATGCTCATGAAAAACCATCAAATGAATGTGGAAGTCCAATGTTGTCAGAAGAACAAAAAGAGATCGTGGAGGGAAAAGAACAAAGAGGTCCCAAGCCAATTAGAGTTCTTGAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGAGTTGGTCAAGTTGTGGCGCTAGACAATGATAAAGCATCTGTCGAAGCTTGTCGGAGAAACATCAAGTTTAATGGTTCAGTGGCATGTTCAAAAGTGGAATCACATCTTACTGATGCTCGTGTTTATATGCTCACTCACCCAAAAGAATTTGATATGGTTGATTTGGATCCTTATGGTTCACCTTCTATTTTCTTGGACTCTGCTGTTCAATCAGTCGTAGATGGTGGCATGCTGATGTGTACAGCAACTGATATGGCAGTGCTTTGTGGGGGAAATGGAGAAGTTTGCTACTCAAAATATGGCTCCTATCCTTTGAGAGGGAAATATTGCCACGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGAGCCATGCAAATAGATACAAGCGGTATATTGTTCCAGTGCTATCTGTTCAGATGGATTTCTATGTCCGGGTTTTTGTTCGTGTTTATACTTCTGCAAGTGCAATGAAAAATACTCCCCTTAAGCTCTCATATGTTTATCAGTGCACTGGTTGTGATTCTTTCCATCTCCAGCAGATTGGCAGAAGCGTCTCCAAGAACAACAGCGTCAGGTATTTACCTGGTTTTGGTCCTTCTGTTGCTCAAGAGTGCAGTGATTGTGGGAAGAAATTCAACATGGGTGGGCCTATTTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATGCTAGCAGATGTGAAGTCTATGAAGGACTCTTATCCTGCATATGATCGTATATCTGCTGTATTGACAACAATTTCCGAGGAGCTGATGGACGTTCCTTTATTTTTGAGTCTACACAACCTCTGTGCAACATTGAAGTGCACATCTCCTTCTGCCGTGAATTTCCGTTCGGCAGTGATTAATGCAGGATATCGCATATCGGGCACTCATGTGAATCCATTAGGTCTGAAATCAGATGCTCCCATGGATGTTATTTGGGATATAATGCGTTGCTGGGTAAAAAACCACCCAGTTAAAACTCAACCAGAAGATCAGGCAGGAAGTATCATTCTTGCCAAGGAACCAGTTCTTGAAGCTAATTTTGCTAGAGCTGTAGCATCTCTTAGCAAGGCACAAGCAAAGAAAATTGCACGCTTCCTTCCAAATCCTGAAAGACATTGGGGCCCCAAGCTCAGAGCAGGTCGTCAAATCACTAGTAAACACATCTCTCTACTTGGTGCAGAGGCAGTAAATGGAGCTCTCAGCCATACGGATTCCGAAGAGCCTAACGCGAAACGTTCCAAGGTAGAAGAAGAACCAGCTCCCAACCAGTAAAAAAAATTCTTTTATCTTATACTTTCTTTTATGCTTCTAAGTTATGCAGTATTACTTTTTGACCCTCCACTTGAAATATTCTTGTTTGGGAACACTTCTACAGAAGTAGAAATTTTTGTTTAATATTGAGGAAGGCTTTCTTTCAAAATATATATCATTTTTGTTCGTCTTTAGGATGTTCAGATTTTTGTCAAGAG

Coding sequence (CDS)

ATGGCGACAGATCTTAGCGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTTATGCACGCGAAAAATGAAGTCTTTTTCAATAAGACCCAGGTTAACAACAGAGATATATCCATTGCTGTCCTTAGGTCATTTATAGCAAAACGAAAACAGGAGCATGAAGCAAAACTATCAAAAAGATCAAAATCAGGGCAGAAGCTTTCTGAAAATAATGGTTCTGAAACTGCTACGGAAGAAGCATGTAATGAATCTGCAACAAATGATGAAAAGTGCGATACAGATTGTGATGCTCATGAAAAACCATCAAATGAATGTGGAAGTCCAATGTTGTCAGAAGAACAAAAAGAGATCGTGGAGGGAAAAGAACAAAGAGGTCCCAAGCCAATTAGAGTTCTTGAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGAGTTGGTCAAGTTGTGGCGCTAGACAATGATAAAGCATCTGTCGAAGCTTGTCGGAGAAACATCAAGTTTAATGGTTCAGTGGCATGTTCAAAAGTGGAATCACATCTTACTGATGCTCGTGTTTATATGCTCACTCACCCAAAAGAATTTGATATGGTTGATTTGGATCCTTATGGTTCACCTTCTATTTTCTTGGACTCTGCTGTTCAATCAGTCGTAGATGGTGGCATGCTGATGTGTACAGCAACTGATATGGCAGTGCTTTGTGGGGGAAATGGAGAAGTTTGCTACTCAAAATATGGCTCCTATCCTTTGAGAGGGAAATATTGCCACGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGAGCCATGCAAATAGATACAAGCGGTATATTGTTCCAGTGCTATCTGTTCAGATGGATTTCTATGTCCGGGTTTTTGTTCGTGTTTATACTTCTGCAAGTGCAATGAAAAATACTCCCCTTAAGCTCTCATATGTTTATCAGTGCACTGGTTGTGATTCTTTCCATCTCCAGCAGATTGGCAGAAGCGTCTCCAAGAACAACAGCGTCAGGTATTTACCTGGTTTTGGTCCTTCTGTTGCTCAAGAGTGCAGTGATTGTGGGAAGAAATTCAACATGGGTGGGCCTATTTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATGCTAGCAGATGTGAAGTCTATGAAGGACTCTTATCCTGCATATGATCGTATATCTGCTGTATTGACAACAATTTCCGAGGAGCTGATGGACGTTCCTTTATTTTTGAGTCTACACAACCTCTGTGCAACATTGAAGTGCACATCTCCTTCTGCCGTGAATTTCCGTTCGGCAGTGATTAATGCAGGATATCGCATATCGGGCACTCATGTGAATCCATTAGGTCTGAAATCAGATGCTCCCATGGATGTTATTTGGGATATAATGCGTTGCTGGGTAAAAAACCACCCAGTTAAAACTCAACCAGAAGATCAGGCAGGAAGTATCATTCTTGCCAAGGAACCAGTTCTTGAAGCTAATTTTGCTAGAGCTGTAGCATCTCTTAGCAAGGCACAAGCAAAGAAAATTGCACGCTTCCTTCCAAATCCTGAAAGACATTGGGGCCCCAAGCTCAGAGCAGGTCGTCAAATCACTAGTAAACACATCTCTCTACTTGGTGCAGAGGCAGTAAATGGAGCTCTCAGCCATACGGATTCCGAAGAGCCTAACGCGAAACGTTCCAAGGTAGAAGAAGAACCAGCTCCCAACCAGTAA

Protein sequence

MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSKRSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVEGKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSDCGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFLSLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ*
Homology
BLAST of CSPI05G29530 vs. ExPASy Swiss-Prot
Match: Q9SRU7 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g02320 PE=2 SV=3)

HSP 1 Score: 865.9 bits (2236), Expect = 2.6e-250
Identity = 435/580 (75.00%), Postives = 489/580 (84.31%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR F++KRKQEHEAK SK
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           R++   K+ E + SE + EE  +E+  N        +   + ++E G   +S++  +  E
Sbjct: 61  RTRPASKVIEKDASEASKEETPSENGMN--------NGDHEVASEDGPSSVSKDPAKTTE 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
               R PKP +VLEALSASGLRALRYARE+EG+GQVVALDND ASVEAC+RNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
            SKVESH TDARV+MLTHPKEFD+VDLDPYGSPSIFLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNSVRYLP  GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAP+HD EWVTS+L  VKSMKD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKC SPSA  FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           P+K Q  +Q GS+IL+KEP  E +F+R + SLSKAQAKK+ARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPN 581
           ITSKH+SL+G EAVNG LS    E    K    E EP  N
Sbjct: 541 ITSKHVSLIGHEAVNGHLSQHHEE---LKEEDEEAEPEDN 569

BLAST of CSPI05G29530 vs. ExPASy Swiss-Prot
Match: Q9LFU5 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g15810 PE=2 SV=3)

HSP 1 Score: 846.3 bits (2185), Expect = 2.1e-244
Identity = 427/577 (74.00%), Postives = 490/577 (84.92%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLR+FI KRKQEHEA LSK
Sbjct: 1   METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           R++S  K+ E + SET+ EE   E+  ++ K + +   HE  + +       +E  +   
Sbjct: 61  RARSSGKVVEKDVSETSKEETPTENGDDNGKTNGE---HEVTTQDG-----PKEAAKTAY 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
              +R  KP RVLEALSASGLRALRYAREVEG+GQVVALDND ASVEAC+RNIKFNG ++
Sbjct: 121 ESARRELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
            SKVESHLTDARV+ML+HPK+FD+VDLDPYG+PSIFLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 181 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNSVRYLPG GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAPIHDQEWV S+L  VKSMKD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLH+L ATLKCTSPSA  FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 421 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           P+K Q  +  GS+IL+KEP  +A+F+R V SLSKAQAKK+ARFLPNPE+HWGPK+RAGR 
Sbjct: 481 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEP 578
           ITSKH+SLLG EAVNG L++   E  + +  + EEEP
Sbjct: 541 ITSKHVSLLGHEAVNGHLNNNHKEAGDEEEEEEEEEP 569

BLAST of CSPI05G29530 vs. ExPASy Swiss-Prot
Match: Q3TX08 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE=1 SV=2)

HSP 1 Score: 378.6 bits (971), Expect = 1.2e-103
Identity = 224/573 (39.09%), Postives = 334/573 (58.29%), Query Frame = 0

Query: 11  IKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSKRSKSGQKLSE 70
           + EG A+I+  + NEVF+N  Q  NRD++ AV+  F    +    AK  +    G+K SE
Sbjct: 58  VTEGAAKIVFPSANEVFYNPVQEFNRDLTCAVITEF---ARIHLGAKGIQIKVPGEKDSE 117

Query: 71  NNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVEGKEQRGPKPI 130
               + + +E   E+A  +E                G    +    EI E       + +
Sbjct: 118 KIAVDLSDQE--EETAGKNENLAP------------GDWPRTAAVGEICE-------EGL 177

Query: 131 RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACSKVESHLTD 190
           RVLE L+ASGLR++R+A EV G+  VVA D    +VE   RN++ NG      V+ +  D
Sbjct: 178 RVLEGLAASGLRSIRFALEVPGLQSVVANDASARAVELMHRNVELNG--VAHLVQPNQAD 237

Query: 191 ARVYMLTH---PKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEV 250
           AR+ M  H   P+ FD++DLDPYGSP+ FLD+AVQ+V DGG+L  T TDMAVL G +GE 
Sbjct: 238 ARMLMYQHQKAPERFDVIDLDPYGSPAPFLDAAVQAVSDGGLLCVTCTDMAVLAGNSGET 297

Query: 251 CYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYTSA 310
           CYSKYG+  L+ + CHEMALRI+L  ++ HAN Y+RYIVP+LS+  DFY+RVFVRV+T  
Sbjct: 298 CYSKYGAMALKSRACHEMALRIVLHSLDLHANCYQRYIVPLLSISADFYIRVFVRVFTGQ 357

Query: 311 SAMKNTPLKLSYVYQCTGCDSFHLQQIGR-SVSKNNSVRYLPGFGPSVAQECSDCGKKFN 370
           + +K++  K + V+QC GC +F+LQ++G+ S      +++    GP V  EC  CG++  
Sbjct: 358 AKVKSSASKQALVFQCVGCGAFYLQRLGKASGDPGGRIKFSAACGPPVTPECEHCGQRHQ 417

Query: 371 MGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFLSLHNLC 430
           +GGP+W+ PIHD ++V  +L  V +    +    RI  VL+ ++EEL DVPL+ +L  L 
Sbjct: 418 LGGPMWAEPIHDLDFVGRVLDAVTTNPGRFHTSMRIQGVLSVVTEELPDVPLYYTLDQLS 477

Query: 431 ATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKTQ- 490
           +T+ C +P  +  RSA+++AG+R+S +H     +K+DAP + +WDIMRCW K  PVK + 
Sbjct: 478 STIHCNTPRLLQLRSALLHAGFRVSLSHACKNAVKTDAPPEALWDIMRCWEKECPVKRER 537

Query: 491 -PEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQITSK 550
             E      ILA EP L+ANF     +   ++ + + RF  NPE +WGP+ RA     + 
Sbjct: 538 LSESSPAFRILAVEPRLKANFNIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAA 597

Query: 551 HISLLGAEAVNGALSHTDSEEPNAKRSKVEEEP 578
              L G   +        +E+P  + ++++  P
Sbjct: 598 SEDLAGRRRLLQNKRKEPAEDPAQRAARLKTFP 604

BLAST of CSPI05G29530 vs. ExPASy Swiss-Prot
Match: Q9NXH9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=1 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 4.4e-101
Identity = 213/535 (39.81%), Postives = 315/535 (58.88%), Query Frame = 0

Query: 9   TIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSKRSKSGQKL 68
           T + EG A+I   + NEVF+N  Q  NRD++ AV+  F         A++   +K  Q  
Sbjct: 55  TTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEF---------ARIQLGAKGIQ-- 114

Query: 69  SENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVEGKEQRGPK 128
            +  G +   +   + S   +EK     +  E  +   G    +    EI E       +
Sbjct: 115 IKVPGEKDTQKVVVDLSEQEEEK----VELKESENLASGDQPRTAAVGEICE-------E 174

Query: 129 PIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACSKVESHL 188
            + VLE L+ASGLR++R+A EV G+  VVA D    +V+  RRN++ N       V+   
Sbjct: 175 GLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLND--VAHLVQPSQ 234

Query: 189 TDARVYMLTHPK---EFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVLCGGNG 248
            DAR+ M  H +    FD++DLDPYGSP+ FLD+AVQ+V +GG+L  T TDMAVL G +G
Sbjct: 235 ADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSG 294

Query: 249 EVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYT 308
           E CYSKYG+  L+ + CHEMALRI+L  ++  AN Y+R++VP+LS+  DFYVRVFVRV+T
Sbjct: 295 ETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFT 354

Query: 309 SASAMKNTPLKLSYVYQCTGCDSFHLQQIGR-SVSKNNSVRYLPGFGPSVAQECSDCGKK 368
             + +K +  K + V+QC GC +FHLQ++G+ S   +   ++    GP V  EC  CG++
Sbjct: 355 GQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQR 414

Query: 369 FNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFLSLHN 428
             +GGP+W+ PIHD ++V  +L  V +    +   +RI  VL+ I+EEL DVPL+ +L  
Sbjct: 415 HQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQ 474

Query: 429 LCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKT 488
           L +T+ C +PS +  RSA+++A +R+S +H     +K+DAP   +WDIMRCW K  PVK 
Sbjct: 475 LSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKR 534

Query: 489 Q--PEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRA 538
           +   E      IL+ EP L+ANF     +   ++ + + RF  NPE +WGP+ RA
Sbjct: 535 ERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRA 565

BLAST of CSPI05G29530 vs. ExPASy Swiss-Prot
Match: Q9VK89 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster OX=7227 GN=CG6388 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 9.2e-99
Identity = 223/542 (41.14%), Postives = 308/542 (56.83%), Query Frame = 0

Query: 10  IIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSKRSKSGQKLS 69
           +I+E  AEI+  +   VF+N  Q  NRD+SIA L  +  +  +E   K  K+ +   K  
Sbjct: 19  VIRERNAEIV--SGGNVFYNPVQEFNRDLSIAALNVYRQRLTKERSEKALKKQRKKVKEQ 78

Query: 70  ENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVEGKEQRGPKP 129
           E                  DEK  T     + P  E G+                R    
Sbjct: 79  E------------------DEK--TTPVPEDPPVYEAGT----------------RYEDG 138

Query: 130 IRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACSKVESHLT 189
           +R+LEAL+A+GLR++RYA+E+ GV Q+VA D  + +V +   NI+ N       +E   +
Sbjct: 139 LRILEALAATGLRSIRYAQEIAGVRQIVANDLSRQAVASINTNIRHN--KVEELIEPSHS 198

Query: 190 DAR--VYMLTHP-KEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVLCGGNGE 249
           DA   +Y+ T P K FD VDLDPYG P+ FLD A+Q +VDGG+L+ TATDMAVL G   E
Sbjct: 199 DAMTLMYLSTQPEKRFDAVDLDPYGCPNRFLDGAMQCLVDGGLLLVTATDMAVLAGNAPE 258

Query: 250 VCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYTS 309
            CY KYGS PLR K CHEMALRILL CIESHANRY +YI P+LS+  DFY+R+FVRVY  
Sbjct: 259 ACYVKYGSVPLRMKCCHEMALRILLHCIESHANRYGKYIEPLLSISADFYIRIFVRVYVG 318

Query: 310 ASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNN-------SVRYLPGFGPSVAQECS 369
            +  K +  K S++YQCTGC++F LQ +G  ++K N        +++    GP+V  +C 
Sbjct: 319 QAQCKLSMSKQSWIYQCTGCETFTLQPLG--ITKPNPTAGNPQQLKFGIPTGPAVNSQCE 378

Query: 370 DCGKKFNMGGPIWSAPIHDQEWVTSMLADV-KSMKDSYPAYDRISAVLTTISEELMDVPL 429
            CG + ++GGPIWSAPIH+ E+V  +L  V ++   S     RI  VL+ + EEL DVPL
Sbjct: 379 HCGHRHHLGGPIWSAPIHNPEFVQDLLTAVQETTLQSLGTQRRIVGVLSMVQEELQDVPL 438

Query: 430 FLSLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVK 489
           + +   LC  LK      + FRSA+++AGYR+S +H +   LK++AP  V+WDI+R W K
Sbjct: 439 YYTPDKLCCVLKLEIVPMLKFRSAILHAGYRVSYSHASKNSLKTNAPPAVLWDILRSWSK 498

Query: 490 NHPVKTQPEDQAGSI--ILAKEPVLEANFARAVASLS-KAQAKKIARFLPNPERHWGPKL 538
            HPV  +       +  IL+KE      F       + K++   ++RF  NP  HWGP  
Sbjct: 499 RHPVNPERMIPGSPLAAILSKECTAVYEFDELHPEANPKSRKSALSRFQENPTPHWGPGT 518

BLAST of CSPI05G29530 vs. ExPASy TrEMBL
Match: A0A0A0KVU4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G641600 PE=3 SV=1)

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE
Sbjct: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 582
           ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 581

BLAST of CSPI05G29530 vs. ExPASy TrEMBL
Match: A0A1S3CA23 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498546 PE=3 SV=1)

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 571/581 (98.28%), Postives = 574/581 (98.80%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           RSKSGQKLSENNGSETATEEA N SATN+EKC TDCDAHEKPSNECGSPMLSEEQKEIVE
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 582
           ITSKHISLLGAEAVNGALSHTDSEEPNAKR KVEEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEEEPAPNQ 581

BLAST of CSPI05G29530 vs. ExPASy TrEMBL
Match: A0A5A7T7H3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002310 PE=3 SV=1)

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 569/581 (97.93%), Postives = 572/581 (98.45%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           M TDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK
Sbjct: 1   MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           RSKSGQKLSENNGSETATEEA N SATN+EKC TDCDAHEKPS ECGSPMLSEEQKEIVE
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 582
           ITSKHISLLGAEAVNGALSHTDSEEPNAKR KVEEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEEEPAPNQ 581

BLAST of CSPI05G29530 vs. ExPASy TrEMBL
Match: A0A6J1GSN6 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456770 PE=3 SV=1)

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 545/581 (93.80%), Postives = 564/581 (97.07%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLR+FI+KRKQEHE K+S+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           R+KS QK+SENNGSE   EEA NESATNDEKC+TDCDAHEK SNECGSP+LSEEQKE V 
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLSEEQKENVA 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKE+R  KPIRVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHP EFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWV+SMLADVKSMKD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVSSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAKEPVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 582
           ITSKHISLLGA+AVNGALSH DSEEPNAKRSKVEE+PAPNQ
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581

BLAST of CSPI05G29530 vs. ExPASy TrEMBL
Match: A0A6J1JNF2 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487439 PE=3 SV=1)

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 546/581 (93.98%), Postives = 562/581 (96.73%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLR+FI+KRKQEHE K+S+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           R+KS QK+SENNGSE   EEA NESATNDEKC+TDCDA EK SNECGSPML E QKE VE
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAREKLSNECGSPMLPEGQKENVE 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKE+R  KPIRVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHP EFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAKEPVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 582
           ITSKHISLLGAEAVNGALSH DSEEPNAKRSKVEE+PAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581

BLAST of CSPI05G29530 vs. NCBI nr
Match: XP_011656015.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis sativus] >KGN52527.1 hypothetical protein Csa_008279 [Cucumis sativus])

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE
Sbjct: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 582
           ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 581

BLAST of CSPI05G29530 vs. NCBI nr
Match: XP_008459398.1 (PREDICTED: probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo])

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 571/581 (98.28%), Postives = 574/581 (98.80%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           RSKSGQKLSENNGSETATEEA N SATN+EKC TDCDAHEKPSNECGSPMLSEEQKEIVE
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 582
           ITSKHISLLGAEAVNGALSHTDSEEPNAKR KVEEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEEEPAPNQ 581

BLAST of CSPI05G29530 vs. NCBI nr
Match: KAA0039424.1 (putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa] >TYK00612.1 putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 569/581 (97.93%), Postives = 572/581 (98.45%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           M TDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK
Sbjct: 1   MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           RSKSGQKLSENNGSETATEEA N SATN+EKC TDCDAHEKPS ECGSPMLSEEQKEIVE
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 582
           ITSKHISLLGAEAVNGALSHTDSEEPNAKR KVEEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEEEPAPNQ 581

BLAST of CSPI05G29530 vs. NCBI nr
Match: XP_038890035.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida])

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 561/581 (96.56%), Postives = 568/581 (97.76%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR FI KRKQEHEAKLSK
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRLFITKRKQEHEAKLSK 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           R+KSGQK++ENNGSET  EEA NESATNDEKC TDC AHEK SNECGSPMLSEEQKE+ E
Sbjct: 61  RAKSGQKVTENNGSETVMEEASNESATNDEKCHTDCGAHEKTSNECGSPMLSEEQKEVAE 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQR PKPIRVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 582
           ITSKHISLLGAEAVNGALSHTDSEEPNAKR KVEEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEEEPAPNQ 581

BLAST of CSPI05G29530 vs. NCBI nr
Match: KAG6602539.1 (putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1095.5 bits (2832), Expect = 0.0e+00
Identity = 546/581 (93.98%), Postives = 564/581 (97.07%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLR+FI+KRKQEHE K+S+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           R+KS QK+SENNGSE   EEA NESATNDEKC+TDCDAHEK SNECGSP+L EEQKE VE
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLPEEQKENVE 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKE+R  KPIRVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHP EFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAKEPVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 582
           ITSKHISLLGA+AVNGALSH DSEEPNAKRSKVEE+PAPNQ
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581

BLAST of CSPI05G29530 vs. TAIR 10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 865.9 bits (2236), Expect = 1.8e-251
Identity = 435/580 (75.00%), Postives = 489/580 (84.31%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR F++KRKQEHEAK SK
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           R++   K+ E + SE + EE  +E+  N        +   + ++E G   +S++  +  E
Sbjct: 61  RTRPASKVIEKDASEASKEETPSENGMN--------NGDHEVASEDGPSSVSKDPAKTTE 120

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
               R PKP +VLEALSASGLRALRYARE+EG+GQVVALDND ASVEAC+RNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
            SKVESH TDARV+MLTHPKEFD+VDLDPYGSPSIFLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNSVRYLP  GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAP+HD EWVTS+L  VKSMKD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKC SPSA  FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           P+K Q  +Q GS+IL+KEP  E +F+R + SLSKAQAKK+ARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPN 581
           ITSKH+SL+G EAVNG LS    E    K    E EP  N
Sbjct: 541 ITSKHVSLIGHEAVNGHLSQHHEE---LKEEDEEAEPEDN 569

BLAST of CSPI05G29530 vs. TAIR 10
Match: AT5G15810.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 846.3 bits (2185), Expect = 1.5e-245
Identity = 427/577 (74.00%), Postives = 490/577 (84.92%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
           M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLR+FI KRKQEHEA LSK
Sbjct: 99  METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 158

Query: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
           R++S  K+ E + SET+ EE   E+  ++ K + +   HE  + +       +E  +   
Sbjct: 159 RARSSGKVVEKDVSETSKEETPTENGDDNGKTNGE---HEVTTQDG-----PKEAAKTAY 218

Query: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
              +R  KP RVLEALSASGLRALRYAREVEG+GQVVALDND ASVEAC+RNIKFNG ++
Sbjct: 219 ESARRELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 278

Query: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
            SKVESHLTDARV+ML+HPK+FD+VDLDPYG+PSIFLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 279 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 338

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 339 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 398

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNSVRYLPG GP V Q+C+ 
Sbjct: 399 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 458

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAPIHDQEWV S+L  VKSMKD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 459 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 518

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLH+L ATLKCTSPSA  FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 519 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 578

Query: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           P+K Q  +  GS+IL+KEP  +A+F+R V SLSKAQAKK+ARFLPNPE+HWGPK+RAGR 
Sbjct: 579 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 638

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEP 578
           ITSKH+SLLG EAVNG L++   E  + +  + EEEP
Sbjct: 639 ITSKHVSLLGHEAVNGHLNNNHKEAGDEEEEEEEEEP 667

BLAST of CSPI05G29530 vs. TAIR 10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 96.3 bits (238), Expect = 8.7e-20
Identity = 92/362 (25.41%), Postives = 151/362 (41.71%), Query Frame = 0

Query: 124 QRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACSK 183
           +R    +RVL+A+   G+R+LRY  E E    V+A D +  +    RR I  N     SK
Sbjct: 84  KRSNGSLRVLDAMCGCGIRSLRYLVEAE-ADFVMANDANDDN----RRVITDN----LSK 143

Query: 184 VESHLTDARVYMLTH-------------PKEFDMVDLDPYGSPSIFLDSAVQSVVDGGML 243
           VE    D R +++TH                FDM+D+D +GS S FL  A  ++  GG+L
Sbjct: 144 VERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALRLGGLL 203

Query: 244 MCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLS 303
             T+TD     G       + YG++     + +E+ LR+L+      A     ++ P+ S
Sbjct: 204 YLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGAVREAALLGYHVTPLFS 263

Query: 304 VQMDFYVRVFVRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGF 363
               ++  VF  +        +      +V  C  C   H  +                 
Sbjct: 264 -YYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRFDEL------------- 323

Query: 364 GPSVAQECSD--CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS----MKDSYPAYDRISA 423
              +   CSD        + GP+W  P+HD  +VT ML   K      + +    D++ +
Sbjct: 324 -GLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLLS 383

Query: 424 VLTTISEELMDVPLFLSLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDA 467
           ++   S+  +  P +  +  + +  K  SP      SA++  GY  S +H+ P  LK+D 
Sbjct: 384 IMIEESDPRLP-PGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDC 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRU72.6e-25075.00Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana O... [more]
Q9LFU52.1e-24474.00Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana O... [more]
Q3TX081.2e-10339.09tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE... [more]
Q9NXH94.4e-10139.81tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=... [more]
Q9VK899.2e-9941.14Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster ... [more]
Match NameE-valueIdentityDescription
A0A0A0KVU40.0e+00100.00tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A1S3CA230.0e+0098.28tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498... [more]
A0A5A7T7H30.0e+0097.93tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1GSN60.0e+0093.80tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JNF20.0e+0093.98tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_011656015.10.0e+00100.00probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis sativus] >KGN525... [more]
XP_008459398.10.0e+0098.28PREDICTED: probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo][more]
KAA0039424.10.0e+0097.93putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa... [more]
XP_038890035.10.0e+0096.56probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida][more]
KAG6602539.10.0e+0093.98putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyr... [more]
Match NameE-valueIdentityDescription
AT3G02320.11.8e-25175.00N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT5G15810.11.5e-24574.00N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT3G56330.18.7e-2025.41N2,N2-dimethylguanosine tRNA methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002905tRNA methyltransferase, Trm1TIGRFAMTIGR00308TIGR00308coord: 11..475
e-value: 4.0E-96
score: 320.6
IPR002905tRNA methyltransferase, Trm1PFAMPF02005TRMcoord: 12..474
e-value: 1.3E-120
score: 403.1
IPR002905tRNA methyltransferase, Trm1PANTHERPTHR10631N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASEcoord: 1..563
IPR002905tRNA methyltransferase, Trm1PROSITEPS51626SAM_MT_TRM1coord: 9..473
score: 138.625732
IPR042296tRNA methyltransferase, Trm1, C-terminalGENE3D3.30.56.70coord: 417..475
e-value: 2.1E-21
score: 77.4
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 8..415
e-value: 7.2E-125
score: 418.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 65..83
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 563..581
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 54..125
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..102
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 556..581
NoneNo IPR availablePANTHERPTHR10631:SF12TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE 1-RELATEDcoord: 1..563
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 8..475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G29530.1CSPI05G29530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002940 tRNA N2-guanine methylation
biological_process GO:0008033 tRNA processing
cellular_component GO:0005634 nucleus
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004809 tRNA (guanine-N2-)-methyltransferase activity
molecular_function GO:0003723 RNA binding