CSPI05G28520 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G28520
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	metalloendoproteinase 2-MMP-like
Location	Chr5: 26899267 .. 26900154 (-)
RNA-Seq Expression	CSPI05G28520
Synteny	CSPI05G28520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGTTCAAGTCTCTCGGATTGCTCTTCCTTGTTCTTGTCACCATTGCATCTCACGTAACCTCCAAACACAACCCAATTCTATCTCCTCAACGTCTCTATGGAGGTCGTAAAAGCGACAACGTAGAAGGAATCCACAACATTAAAAAATACCTTCAACGTTATGGTTACTTATCACACAACATGACTACTGATTCCCATATCATTGAACTCAACAGCAACAAGTTCGACGACTCCCTAGAATCTGCCATTAAATTATATCAGAAATGGTCTCACCTCAACGTAAGTGGGATTCTAGACCAAGAAACATTAGATCAAATGTTCCAACCACGATGTGGCGTACGAGATGTCTTCAAATTTAACTCAAGTAAGAATCTTGAGGATGATCTTGAAATGTCATCTCATTACGCTTTATTCCCAGGCAACCTAAAATGGCCAGATTACAAACGCCACCTAACCTACGTATTTACCAACAATTTCCCAATTAACTTTGTGCCATCAGTGACTGAGGCTATGGCTCGATGGGCAGCCCATTCACTATTCACATTCTCAGAAGCTTCGGATGCCCAAAGTGCTGACATTAATATCAGCTTTCAAATAAAGGATCATGCAGATGGGATGCCTTTTGATGGACCTGGAGGAGTTGTGGGTCATGCTTTTGCACCAATTGATGGAAGATTACACTTAGATGGCGACGATAGTTGGTCGGCAGGGTTGGAAGAGAAGAAGGTTAATGTGATGAATGCGGCACTTCATGAGCTTGGGCATGTTCTTGGTCTTGCCCATAGCACCCTTCCACAAGCTGTCATGTGGCCCTATATTGAATCCAATGCTTTAAAGAACTTGAATGATGATGATATTGCCGGACTTCATGCTTTGTACCCTTAA

mRNA sequence

ATGACGTTCAAGTCTCTCGGATTGCTCTTCCTTGTTCTTGTCACCATTGCATCTCACGTAACCTCCAAACACAACCCAATTCTATCTCCTCAACGTCTCTATGGAGGTCGTAAAAGCGACAACGTAGAAGGAATCCACAACATTAAAAAATACCTTCAACGTTATGGTTACTTATCACACAACATGACTACTGATTCCCATATCATTGAACTCAACAGCAACAAGTTCGACGACTCCCTAGAATCTGCCATTAAATTATATCAGAAATGGTCTCACCTCAACGTAAGTGGGATTCTAGACCAAGAAACATTAGATCAAATGTTCCAACCACGATGTGGCGTACGAGATGTCTTCAAATTTAACTCAAGTAAGAATCTTGAGGATGATCTTGAAATGTCATCTCATTACGCTTTATTCCCAGGCAACCTAAAATGGCCAGATTACAAACGCCACCTAACCTACGTATTTACCAACAATTTCCCAATTAACTTTGTGCCATCAGTGACTGAGGCTATGGCTCGATGGGCAGCCCATTCACTATTCACATTCTCAGAAGCTTCGGATGCCCAAAGTGCTGACATTAATATCAGCTTTCAAATAAAGGATCATGCAGATGGGATGCCTTTTGATGGACCTGGAGGAGTTGTGGGTCATGCTTTTGCACCAATTGATGGAAGATTACACTTAGATGGCGACGATAGTTGGTCGGCAGGGTTGGAAGAGAAGAAGGTTAATGTGATGAATGCGGCACTTCATGAGCTTGGGCATGTTCTTGGTCTTGCCCATAGCACCCTTCCACAAGCTGTCATGTGGCCCTATATTGAATCCAATGCTTTAAAGAACTTGAATGATGATGATATTGCCGGACTTCATGCTTTGTACCCTTAA

Coding sequence (CDS)

ATGACGTTCAAGTCTCTCGGATTGCTCTTCCTTGTTCTTGTCACCATTGCATCTCACGTAACCTCCAAACACAACCCAATTCTATCTCCTCAACGTCTCTATGGAGGTCGTAAAAGCGACAACGTAGAAGGAATCCACAACATTAAAAAATACCTTCAACGTTATGGTTACTTATCACACAACATGACTACTGATTCCCATATCATTGAACTCAACAGCAACAAGTTCGACGACTCCCTAGAATCTGCCATTAAATTATATCAGAAATGGTCTCACCTCAACGTAAGTGGGATTCTAGACCAAGAAACATTAGATCAAATGTTCCAACCACGATGTGGCGTACGAGATGTCTTCAAATTTAACTCAAGTAAGAATCTTGAGGATGATCTTGAAATGTCATCTCATTACGCTTTATTCCCAGGCAACCTAAAATGGCCAGATTACAAACGCCACCTAACCTACGTATTTACCAACAATTTCCCAATTAACTTTGTGCCATCAGTGACTGAGGCTATGGCTCGATGGGCAGCCCATTCACTATTCACATTCTCAGAAGCTTCGGATGCCCAAAGTGCTGACATTAATATCAGCTTTCAAATAAAGGATCATGCAGATGGGATGCCTTTTGATGGACCTGGAGGAGTTGTGGGTCATGCTTTTGCACCAATTGATGGAAGATTACACTTAGATGGCGACGATAGTTGGTCGGCAGGGTTGGAAGAGAAGAAGGTTAATGTGATGAATGCGGCACTTCATGAGCTTGGGCATGTTCTTGGTCTTGCCCATAGCACCCTTCCACAAGCTGTCATGTGGCCCTATATTGAATCCAATGCTTTAAAGAACTTGAATGATGATGATATTGCCGGACTTCATGCTTTGTACCCTTAA

Protein sequence

MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSHNMTTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLEEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP*

Homology

[Close]

BLAST of CSPI05G28520 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 9.6e-44
Identity = 105/275 (38.18%), Postives = 147/275 (53.45%), Query Frame = 0


Query: 32  RLYGGRKSDNVEGIHNIKKYLQRYGYLSHNMTTDSHIIELNSNKFDDSLESAIKLYQKWS 91
           +L G    +N+ G+  +K+Y +R+GY+    TT  +     ++ FDD L+SAI  YQK  
Sbjct: 47  KLAGCHIGENINGLSKLKQYFRRFGYI----TTTGNC----TDDFDDVLQSAINTYQKNF 106

Query: 92  HLNVSGILDQETLDQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWPDYKRH 151
           +L V+G LD  TL Q+ +PRCG  D+    S  N    L  +  Y+ FPG  +WP  KR 
Sbjct: 107 NLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRD 166

Query: 152 LTYVFT--NNFPINFVPSVTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHADGMPF 211
           LTY F   NN         + A  RWA  +   F+ +     ADI I F   +H DG PF
Sbjct: 167 LTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPF 226

Query: 212 DGPGGVVGHAFAPIDGRLHLDGDDSW--SAGLEEKK-------VNVMNAALHELGHVLGL 271
           DG  G + HA +P  G LHLDGD+ W  S G   ++       V++ + A+HE+GH+LGL
Sbjct: 227 DGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGL 286

Query: 272 AHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 295
            HS++  A+M+P I     K  L  DDI G+  LY
Sbjct: 287 GHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of CSPI05G28520 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.3e-43
Identity = 107/288 (37.15%), Postives = 155/288 (53.82%), Query Frame = 0


Query: 35  GGRKSDNVEGIHNIKKYLQRYGYL----SHNMTTDSHIIELNSNKFDDSLESAIKLYQKW 94
           G     NV+G++ IKKY QR+GY+    S N T D          FDD L++A++LYQ  
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDD----------FDDILKAAVELYQTN 108

Query: 95  SHLNVSGILDQETLDQMFQPRCGVRDV--------------FKFNSSKNLEDDLEMSSHY 154
            +LNV+G LD  T+  +  PRCG  DV              F+ N S+     L     Y
Sbjct: 109 FNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRT---HLHAVKRY 168

Query: 155 ALFPGNLKWPDYKRHLTYVFTNNFPI-NFVPSV-TEAMARWAAHSLFTFSEASDAQSADI 214
            LFPG  +WP  +R LTY F    P+   V SV + A  RW+  +   F+ +    ++DI
Sbjct: 169 TLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDI 228

Query: 215 NISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSW--SAGLEE-----KKVNVM 274
            I F   DH DG PFDG  G + HAF+P  G+ HLD D++W  S  L+        V++ 
Sbjct: 229 TIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLE 288

Query: 275 NAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 295
           + A+HE+GH+LGL HS++ +++M+P I +   K +L +DD+ G+  LY
Sbjct: 289 SVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of CSPI05G28520 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 178.3 bits (451), Expect = 1.3e-43
Identity = 103/263 (39.16%), Postives = 142/263 (53.99%), Query Frame = 0


Query: 41  NVEGIHNIKKYLQRYGYLSHNMTTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILD 100
           N +G+ N+K Y    GY+ +    D        + FDD+L SAIK YQK  +LNV+G  D
Sbjct: 50  NYKGLSNVKNYFHHLGYIPNAPHFD--------DNFDDTLVSAIKTYQKNYNLNVTGKFD 109

Query: 101 QETLDQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNF 160
             TL Q+  PRCGV D+   N++K       M S Y  F    +W      LTY F+   
Sbjct: 110 INTLKQIMTPRCGVPDII-INTNKT--TSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEP 169

Query: 161 PIN--FVPSVTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGH 220
            ++  F  ++  A ++W       F E +  ++A+I I F  K+H D  PFDGPGG++GH
Sbjct: 170 RLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGH 229

Query: 221 AFAPIDGRLHLDGDDSWSAGLEEKKVNVMNA------ALHELGHVLGLAHSTLPQAVMWP 280
           AFAP DGR H D D+ W A  +  K  V +A      A+HE+GH+LGL HS+  +A+M+P
Sbjct: 230 AFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYP 289

Query: 281 YIESNALK-NLNDDDIAGLHALY 295
            I     K NL  DDI G+  LY
Sbjct: 290 SIPPRTRKVNLAQDDIDGIRKLY 301

[Close]

BLAST of CSPI05G28520 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.0e-42
Identity = 101/293 (34.47%), Postives = 160/293 (54.61%), Query Frame = 0


Query: 25  NPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSHNMTTDSHIIELNSNKFDDSLESAI 84
           N  L+    + G+K D   G++ +K+Y Q +GY+     T++++    ++ FDD L++A+
Sbjct: 44  NSFLNFTGCHAGKKYD---GLYMLKQYFQHFGYI-----TETNLSGNFTDDFDDILKNAV 103

Query: 85  KLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNSSKNL-EDDLEMS---------- 144
           ++YQ+   LNV+G+LD+ TL  +  PRCG  DV    S+ +      E+S          
Sbjct: 104 EMYQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHA 163

Query: 145 -SHYALFPGNLKWPDYKRHLTYVF-TNNFPINFVPSV-TEAMARWAAHSLFTFSEASDAQ 204
             HY+ FPG  +WP  +R LTY F   N     V SV + A  RW   +  TF+      
Sbjct: 164 VKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFS 223

Query: 205 SADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE--------EK 264
           ++DI+I F   +H DG PFDGP   + HAF+P  G  HLDG+++W    E         +
Sbjct: 224 TSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSE 283

Query: 265 KVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 295
            V++ + A+HE+GH+LGL HS++  ++M+P I +   K +L  DD+ G+  LY
Sbjct: 284 AVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of CSPI05G28520 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.5e-41
Identity = 101/275 (36.73%), Postives = 148/275 (53.82%), Query Frame = 0


Query: 32  RLYGGRKSDNVEGIHNIKKYLQRYGYLSHNMTTDSHIIELNSNKFDDSLESAIKLYQKWS 91
           RL   +   +V G+  +K+YL R+GY++          E+ S+ FD  LESAI LYQ+  
Sbjct: 57  RLVDVQIGSHVSGVSELKRYLHRFGYVNDGS-------EIFSDVFDGPLESAISLYQENL 116

Query: 92  HLNVSGILDQETLDQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWPDYKRH 151
            L ++G LD  T+  M  PRCGV D     ++    D L  ++HY  F G  KW   +  
Sbjct: 117 GLPITGRLDTSTVTLMSLPRCGVSDTHMTINN----DFLHTTAHYTYFNGKPKW--NRDT 176

Query: 152 LTYVFTNNFPINFVPS------VTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHAD 211
           LTY  +    ++++ S         A ++W++    +F E  D  +AD+ I F   DH D
Sbjct: 177 LTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGD 236

Query: 212 GMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSA-----GLEEKKVNVMNAALHELGHVLGL 271
           G+PFDG  G + HAFAP +GRLHLD  ++W       G  E  V++ + A HE+GH+LGL
Sbjct: 237 GLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGL 296

Query: 272 AHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 295
            HS+   AVM+P +     K +L  DD+AG+  LY
Sbjct: 297 GHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of CSPI05G28520 vs. ExPASy TrEMBL
Match: A0A0A0KNY8 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G522910 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.3e-159
Identity = 272/295 (92.20%), Postives = 284/295 (96.27%), Query Frame = 0


Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSH 60
           M FKSL LLFLVLVTIASHV+SKHNPILSPQRLYG RK DNVEGIH+IKKYLQRYGYLSH
Sbjct: 1   MAFKSLQLLFLVLVTIASHVSSKHNPILSPQRLYGCRKGDNVEGIHSIKKYLQRYGYLSH 60

Query: 61  NMTTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKF 120
           N + DSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMF+PRCGVRDVFKF
Sbjct: 61  NTSIDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFKPRCGVRDVFKF 120

Query: 121 NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL 180
           NS+KNLEDDLEMSSHY LFPGNLKWPDYKRHLTYVFTNNFP++FVPSVTEAMARWAA SL
Sbjct: 121 NSNKNLEDDLEMSSHYTLFPGNLKWPDYKRHLTYVFTNNFPVDFVPSVTEAMARWAAQSL 180

Query: 181 FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE 240
           FTFSEASDAQSADINISFQIKDHADG+PFDGPGGVVGHAFAP DGRLHLDGDD+WSAG+E
Sbjct: 181 FTFSEASDAQSADINISFQIKDHADGLPFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGME 240

Query: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 296
            +KVNVMN ALHELGHVLGLAHSTLPQAVMWPYI+SNALKNLNDDDIAGLHALYP
Sbjct: 241 VQKVNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALYP 295

[Close]

BLAST of CSPI05G28520 vs. ExPASy TrEMBL
Match: A0A0A0KX71 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G631560 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.4e-154
Identity = 266/295 (90.17%), Postives = 280/295 (94.92%), Query Frame = 0


Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSH 60
           M FKSL LLFL+L +IASHV SKHN ILSP+RLYG RK DNVEGIH+IKKYLQRYGYLSH
Sbjct: 1   MAFKSLQLLFLILASIASHVISKHNSILSPRRLYGCRKDDNVEGIHSIKKYLQRYGYLSH 60

Query: 61  NMTTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKF 120
           N +TDS+IIELNSNKFDDSLESAI+LYQKWSHLNVSGI+DQETLDQMFQPRCGV DVFKF
Sbjct: 61  NTSTDSNIIELNSNKFDDSLESAIRLYQKWSHLNVSGIIDQETLDQMFQPRCGVPDVFKF 120

Query: 121 NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL 180
           NSSKNLEDDLEMSSHYALFP N KWPDYKRHLTY+FTNNFPI+FVPS+TEAMARWAA SL
Sbjct: 121 NSSKNLEDDLEMSSHYALFPNNEKWPDYKRHLTYMFTNNFPIDFVPSMTEAMARWAAQSL 180

Query: 181 FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE 240
           FTFSEASDAQSADINISFQ+KDHADG  FDGPGGVVGHAFAP DGRLHLDGDD+WSAGLE
Sbjct: 181 FTFSEASDAQSADINISFQVKDHADGFAFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGLE 240

Query: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 296
           EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAG+HALYP
Sbjct: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGIHALYP 295

[Close]

BLAST of CSPI05G28520 vs. ExPASy TrEMBL
Match: A0A0A0KVD8 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633150 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.0e-149
Identity = 260/292 (89.04%), Postives = 272/292 (93.15%), Query Frame = 0


Query: 4   KSLGLLFLVLVTIASHVTSKHNPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSHNMT 63
           KSL LLFLVL +IASHV S +N ILSP+RL G RK DNVEGIHNIKKYLQRYGYLSHN +
Sbjct: 3   KSLQLLFLVLASIASHVISINNQILSPRRLNGCRKGDNVEGIHNIKKYLQRYGYLSHNTS 62

Query: 64  TDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNSS 123
           TDS+IIELNSNKFDDSLESAI+LYQKWSHLNVSGILDQETLDQMFQ RCG+RDVFKFNSS
Sbjct: 63  TDSNIIELNSNKFDDSLESAIRLYQKWSHLNVSGILDQETLDQMFQQRCGIRDVFKFNSS 122

Query: 124 KNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSLFTF 183
           KNLEDDLEMSSHY LFP N KWPDYKRHLTY+FTNNFP++FVPSVTEAMARWAA SLFTF
Sbjct: 123 KNLEDDLEMSSHYVLFPNNKKWPDYKRHLTYMFTNNFPVDFVPSVTEAMARWAAQSLFTF 182

Query: 184 SEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLEEKK 243
           SEASDAQSADINISFQ+KDHADG  FDGPGGVVGHAFAP DGRLHLDGDDSWSAGLEE K
Sbjct: 183 SEASDAQSADINISFQVKDHADGSAFDGPGGVVGHAFAPTDGRLHLDGDDSWSAGLEENK 242

Query: 244 VNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 296
            NVMN ALHELGHVLGLAHSTLPQAVMWPYI+SNALKNLNDDDIAGLHALYP
Sbjct: 243 FNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALYP 294

[Close]

BLAST of CSPI05G28520 vs. ExPASy TrEMBL
Match: A0A5A7T3V3 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00350 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 4.8e-131
Identity = 232/296 (78.38%), Postives = 252/296 (85.14%), Query Frame = 0


Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSH 60
           M FKSL L FL+L +  SHV S+H P   PQ LYG RK DNVEGIH+IKKYL RYGYLSH
Sbjct: 1   MAFKSLQLFFLLLASFTSHVISRHIPTPIPQHLYGCRKGDNVEGIHSIKKYLHRYGYLSH 60

Query: 61  NMTTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDV-FK 120
           N  T SH IELNSN FDD+LESA+KLYQKWS LNVSGILDQ+TLDQ+F+PRCG+ DV  K
Sbjct: 61  NTNTSSH-IELNSNTFDDTLESAVKLYQKWSKLNVSGILDQQTLDQIFKPRCGLPDVIMK 120

Query: 121 FNSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHS 180
            NS+KN EDDLE+SSHYALFPGN KWPDYKRHLTY FTNNFPI+FVPSVTEAM  WAAHS
Sbjct: 121 SNSNKNTEDDLEISSHYALFPGNQKWPDYKRHLTYTFTNNFPIDFVPSVTEAMKEWAAHS 180

Query: 181 LFTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGL 240
           LFTFS+A+D Q  DINISFQ+ DH DG+PFDGPGGVVGHAFAP DGRLHLDGDD WSAGL
Sbjct: 181 LFTFSQAADDQVPDINISFQVGDHGDGVPFDGPGGVVGHAFAPTDGRLHLDGDDKWSAGL 240

Query: 241 EEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 296
           E +KVNVMNAALHELGHVLGLAHST+ QAVMWPYI+  ALK L DDDIAGL ALYP
Sbjct: 241 ETQKVNVMNAALHELGHVLGLAHSTVQQAVMWPYIDVYALKALTDDDIAGLTALYP 295

[Close]

BLAST of CSPI05G28520 vs. ExPASy TrEMBL
Match: A0A1S3C9J6 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498179 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 4.8e-131
Identity = 232/296 (78.38%), Postives = 252/296 (85.14%), Query Frame = 0


Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSH 60
           M FKSL L FL+L +  SHV S+H P   PQ LYG RK DNVEGIH+IKKYL RYGYLSH
Sbjct: 1   MAFKSLQLFFLLLASFTSHVISRHIPTPIPQHLYGCRKGDNVEGIHSIKKYLHRYGYLSH 60

Query: 61  NMTTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDV-FK 120
           N  T SH IELNSN FDD+LESA+KLYQKWS LNVSGILDQ+TLDQ+F+PRCG+ DV  K
Sbjct: 61  NTNTSSH-IELNSNTFDDTLESAVKLYQKWSKLNVSGILDQQTLDQIFKPRCGLPDVIMK 120

Query: 121 FNSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHS 180
            NS+KN EDDLE+SSHYALFPGN KWPDYKRHLTY FTNNFPI+FVPSVTEAM  WAAHS
Sbjct: 121 SNSNKNTEDDLEISSHYALFPGNQKWPDYKRHLTYTFTNNFPIDFVPSVTEAMKEWAAHS 180

Query: 181 LFTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGL 240
           LFTFS+A+D Q  DINISFQ+ DH DG+PFDGPGGVVGHAFAP DGRLHLDGDD WSAGL
Sbjct: 181 LFTFSQAADDQVPDINISFQVGDHGDGVPFDGPGGVVGHAFAPTDGRLHLDGDDKWSAGL 240

Query: 241 EEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 296
           E +KVNVMNAALHELGHVLGLAHST+ QAVMWPYI+  ALK L DDDIAGL ALYP
Sbjct: 241 ETQKVNVMNAALHELGHVLGLAHSTVQQAVMWPYIDVYALKALTDDDIAGLTALYP 295

[Close]

BLAST of CSPI05G28520 vs. NCBI nr
Match: XP_031741767.1 (metalloendoproteinase 5-MMP-like [Cucumis sativus] >KAE8648903.1 hypothetical protein Csa_008730 [Cucumis sativus])

HSP 1 Score: 596.3 bits (1536), Expect = 1.5e-166
Identity = 291/295 (98.64%), Postives = 291/295 (98.64%), Query Frame = 0


Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSH 60
           MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYG RK DNVEGIHNIKKYLQRYGYLSH
Sbjct: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSH 60

Query: 61  NMTTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKF 120
           N  TDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKF
Sbjct: 61  N--TDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKF 120

Query: 121 NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL 180
           NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL
Sbjct: 121 NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL 180

Query: 181 FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE 240
           FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE
Sbjct: 181 FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE 240

Query: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 296
           EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP
Sbjct: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 293

[Close]

BLAST of CSPI05G28520 vs. NCBI nr
Match: XP_004142458.3 (metalloendoproteinase 1 [Cucumis sativus] >KAE8648904.1 hypothetical protein Csa_009110 [Cucumis sativus])

HSP 1 Score: 570.9 bits (1470), Expect = 6.6e-159
Identity = 274/295 (92.88%), Postives = 283/295 (95.93%), Query Frame = 0


Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSH 60
           M FKSL LLFLV V+IASHV SKHNPILSPQRLYG RK DNVEGI NIKKYLQRYGYLSH
Sbjct: 1   MAFKSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSH 60

Query: 61  NMTTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKF 120
           NM+ DSHIIELNSNKFDDSLESAIKLYQKW+HLNVSG+LDQETLDQMF+PRCGVRDVFKF
Sbjct: 61  NMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLNVSGVLDQETLDQMFKPRCGVRDVFKF 120

Query: 121 NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL 180
           NSSKNLEDDLEMSSHYALFPGN +WPDYKRHLTYVFTNNFPI+FVPSVTEAMARWA  SL
Sbjct: 121 NSSKNLEDDLEMSSHYALFPGNPQWPDYKRHLTYVFTNNFPIDFVPSVTEAMARWAGQSL 180

Query: 181 FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE 240
           FTFSEASDAQSADINISFQIKDHADG+PFDGPGG+VGHAFAP DGRLHLDGDDSWSAGLE
Sbjct: 181 FTFSEASDAQSADINISFQIKDHADGLPFDGPGGIVGHAFAPTDGRLHLDGDDSWSAGLE 240

Query: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 296
           EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP
Sbjct: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 295

[Close]

BLAST of CSPI05G28520 vs. NCBI nr
Match: XP_004142843.3 (metalloendoproteinase 1 [Cucumis sativus] >KAE8648550.1 hypothetical protein Csa_008112 [Cucumis sativus])

HSP 1 Score: 569.7 bits (1467), Expect = 1.5e-158
Identity = 272/295 (92.20%), Postives = 285/295 (96.61%), Query Frame = 0


Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSH 60
           M FKSL LLFLVLVTIASHV+SKHNPILSPQRLYG RK DNVEGIH+IKKYLQRYGYLSH
Sbjct: 1   MAFKSLQLLFLVLVTIASHVSSKHNPILSPQRLYGCRKGDNVEGIHSIKKYLQRYGYLSH 60

Query: 61  NMTTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKF 120
           N + DSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMF+PRCGVRDVFKF
Sbjct: 61  NTSIDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFKPRCGVRDVFKF 120

Query: 121 NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL 180
           NS+KNLEDDLEMSSHY LFPGNLKWPDYKRHLTYVFTNNFPI+F+PSVTEAMARWAA SL
Sbjct: 121 NSNKNLEDDLEMSSHYTLFPGNLKWPDYKRHLTYVFTNNFPIDFMPSVTEAMARWAAQSL 180

Query: 181 FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE 240
           FTFSEASDAQSADINISFQIKDHADG+PFDGPGGVVGHAFAP DGRLHLDGDD+WSAG+E
Sbjct: 181 FTFSEASDAQSADINISFQIKDHADGLPFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGME 240

Query: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 296
            +KVNVMNAALHELGH+LGLAHSTLPQAVMWPYI+SNALKNLNDDDIAGLHALYP
Sbjct: 241 VQKVNVMNAALHELGHILGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALYP 295

[Close]

BLAST of CSPI05G28520 vs. NCBI nr
Match: XP_004142460.2 (metalloendoproteinase 1 [Cucumis sativus] >KGN52406.1 hypothetical protein Csa_008066 [Cucumis sativus])

HSP 1 Score: 555.4 bits (1430), Expect = 2.9e-154
Identity = 266/295 (90.17%), Postives = 280/295 (94.92%), Query Frame = 0


Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSH 60
           M FKSL LLFL+L +IASHV SKHN ILSP+RLYG RK DNVEGIH+IKKYLQRYGYLSH
Sbjct: 1   MAFKSLQLLFLILASIASHVISKHNSILSPRRLYGCRKDDNVEGIHSIKKYLQRYGYLSH 60

Query: 61  NMTTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKF 120
           N +TDS+IIELNSNKFDDSLESAI+LYQKWSHLNVSGI+DQETLDQMFQPRCGV DVFKF
Sbjct: 61  NTSTDSNIIELNSNKFDDSLESAIRLYQKWSHLNVSGIIDQETLDQMFQPRCGVPDVFKF 120

Query: 121 NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL 180
           NSSKNLEDDLEMSSHYALFP N KWPDYKRHLTY+FTNNFPI+FVPS+TEAMARWAA SL
Sbjct: 121 NSSKNLEDDLEMSSHYALFPNNEKWPDYKRHLTYMFTNNFPIDFVPSMTEAMARWAAQSL 180

Query: 181 FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE 240
           FTFSEASDAQSADINISFQ+KDHADG  FDGPGGVVGHAFAP DGRLHLDGDD+WSAGLE
Sbjct: 181 FTFSEASDAQSADINISFQVKDHADGFAFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGLE 240

Query: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 296
           EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAG+HALYP
Sbjct: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGIHALYP 295

[Close]

BLAST of CSPI05G28520 vs. NCBI nr
Match: XP_011656276.2 (metalloendoproteinase 1 [Cucumis sativus] >KAE8648905.1 hypothetical protein Csa_008996 [Cucumis sativus])

HSP 1 Score: 542.3 bits (1396), Expect = 2.5e-150
Identity = 261/292 (89.38%), Postives = 273/292 (93.49%), Query Frame = 0


Query: 4   KSLGLLFLVLVTIASHVTSKHNPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSHNMT 63
           KSL LLFLVL +IASHV S +N ILSP+RLYG RK DNVEGIHNIKKYLQRYGYLSHN +
Sbjct: 3   KSLQLLFLVLASIASHVISINNQILSPRRLYGCRKGDNVEGIHNIKKYLQRYGYLSHNTS 62

Query: 64  TDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNSS 123
           TDS+IIELNSNKFDDSLESAI+LYQKWSHLNVSGILDQETLDQMFQ RCG+RDVFKFNSS
Sbjct: 63  TDSNIIELNSNKFDDSLESAIRLYQKWSHLNVSGILDQETLDQMFQQRCGIRDVFKFNSS 122

Query: 124 KNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSLFTF 183
           KNLEDDLEMSSHY LFP N KWPDYKRHLTY+FTNNFP++FVPSVTEAMARWAA SLFTF
Sbjct: 123 KNLEDDLEMSSHYVLFPNNEKWPDYKRHLTYMFTNNFPVDFVPSVTEAMARWAAQSLFTF 182

Query: 184 SEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLEEKK 243
           SEASDAQSADINISFQ+KDHADG  FDGPGGVVGHAFAP DGRLHLDGDDSWSAGLEE K
Sbjct: 183 SEASDAQSADINISFQVKDHADGSAFDGPGGVVGHAFAPTDGRLHLDGDDSWSAGLEENK 242

Query: 244 VNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 296
            NVMN ALHELGHVLGLAHSTLPQAVMWPYI+SNALKNLNDDDIAGLHALYP
Sbjct: 243 FNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALYP 294

[Close]

BLAST of CSPI05G28520 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 178.7 bits (452), Expect = 6.8e-45
Identity = 105/275 (38.18%), Postives = 147/275 (53.45%), Query Frame = 0


Query: 32  RLYGGRKSDNVEGIHNIKKYLQRYGYLSHNMTTDSHIIELNSNKFDDSLESAIKLYQKWS 91
           +L G    +N+ G+  +K+Y +R+GY+    TT  +     ++ FDD L+SAI  YQK  
Sbjct: 47  KLAGCHIGENINGLSKLKQYFRRFGYI----TTTGNC----TDDFDDVLQSAINTYQKNF 106

Query: 92  HLNVSGILDQETLDQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWPDYKRH 151
           +L V+G LD  TL Q+ +PRCG  D+    S  N    L  +  Y+ FPG  +WP  KR 
Sbjct: 107 NLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRD 166

Query: 152 LTYVFT--NNFPINFVPSVTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHADGMPF 211
           LTY F   NN         + A  RWA  +   F+ +     ADI I F   +H DG PF
Sbjct: 167 LTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPF 226

Query: 212 DGPGGVVGHAFAPIDGRLHLDGDDSW--SAGLEEKK-------VNVMNAALHELGHVLGL 271
           DG  G + HA +P  G LHLDGD+ W  S G   ++       V++ + A+HE+GH+LGL
Sbjct: 227 DGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGL 286

Query: 272 AHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 295
            HS++  A+M+P I     K  L  DDI G+  LY
Sbjct: 287 GHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of CSPI05G28520 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 178.3 bits (451), Expect = 8.9e-45
Identity = 107/288 (37.15%), Postives = 155/288 (53.82%), Query Frame = 0


Query: 35  GGRKSDNVEGIHNIKKYLQRYGYL----SHNMTTDSHIIELNSNKFDDSLESAIKLYQKW 94
           G     NV+G++ IKKY QR+GY+    S N T D          FDD L++A++LYQ  
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDD----------FDDILKAAVELYQTN 108

Query: 95  SHLNVSGILDQETLDQMFQPRCGVRDV--------------FKFNSSKNLEDDLEMSSHY 154
            +LNV+G LD  T+  +  PRCG  DV              F+ N S+     L     Y
Sbjct: 109 FNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRT---HLHAVKRY 168

Query: 155 ALFPGNLKWPDYKRHLTYVFTNNFPI-NFVPSV-TEAMARWAAHSLFTFSEASDAQSADI 214
            LFPG  +WP  +R LTY F    P+   V SV + A  RW+  +   F+ +    ++DI
Sbjct: 169 TLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDI 228

Query: 215 NISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSW--SAGLEE-----KKVNVM 274
            I F   DH DG PFDG  G + HAF+P  G+ HLD D++W  S  L+        V++ 
Sbjct: 229 TIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLE 288

Query: 275 NAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 295
           + A+HE+GH+LGL HS++ +++M+P I +   K +L +DD+ G+  LY
Sbjct: 289 SVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of CSPI05G28520 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 173.3 bits (438), Expect = 2.9e-43
Identity = 101/293 (34.47%), Postives = 160/293 (54.61%), Query Frame = 0


Query: 25  NPILSPQRLYGGRKSDNVEGIHNIKKYLQRYGYLSHNMTTDSHIIELNSNKFDDSLESAI 84
           N  L+    + G+K D   G++ +K+Y Q +GY+     T++++    ++ FDD L++A+
Sbjct: 44  NSFLNFTGCHAGKKYD---GLYMLKQYFQHFGYI-----TETNLSGNFTDDFDDILKNAV 103

Query: 85  KLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNSSKNL-EDDLEMS---------- 144
           ++YQ+   LNV+G+LD+ TL  +  PRCG  DV    S+ +      E+S          
Sbjct: 104 EMYQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHA 163

Query: 145 -SHYALFPGNLKWPDYKRHLTYVF-TNNFPINFVPSV-TEAMARWAAHSLFTFSEASDAQ 204
             HY+ FPG  +WP  +R LTY F   N     V SV + A  RW   +  TF+      
Sbjct: 164 VKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFS 223

Query: 205 SADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE--------EK 264
           ++DI+I F   +H DG PFDGP   + HAF+P  G  HLDG+++W    E         +
Sbjct: 224 TSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSE 283

Query: 265 KVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 295
            V++ + A+HE+GH+LGL HS++  ++M+P I +   K +L  DD+ G+  LY
Sbjct: 284 AVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of CSPI05G28520 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.1e-42
Identity = 101/275 (36.73%), Postives = 148/275 (53.82%), Query Frame = 0


Query: 32  RLYGGRKSDNVEGIHNIKKYLQRYGYLSHNMTTDSHIIELNSNKFDDSLESAIKLYQKWS 91
           RL   +   +V G+  +K+YL R+GY++          E+ S+ FD  LESAI LYQ+  
Sbjct: 57  RLVDVQIGSHVSGVSELKRYLHRFGYVNDGS-------EIFSDVFDGPLESAISLYQENL 116

Query: 92  HLNVSGILDQETLDQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWPDYKRH 151
            L ++G LD  T+  M  PRCGV D     ++    D L  ++HY  F G  KW   +  
Sbjct: 117 GLPITGRLDTSTVTLMSLPRCGVSDTHMTINN----DFLHTTAHYTYFNGKPKW--NRDT 176

Query: 152 LTYVFTNNFPINFVPS------VTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHAD 211
           LTY  +    ++++ S         A ++W++    +F E  D  +AD+ I F   DH D
Sbjct: 177 LTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGD 236

Query: 212 GMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSA-----GLEEKKVNVMNAALHELGHVLGL 271
           G+PFDG  G + HAFAP +GRLHLD  ++W       G  E  V++ + A HE+GH+LGL
Sbjct: 237 GLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGL 296

Query: 272 AHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 295
            HS+   AVM+P +     K +L  DD+AG+  LY
Sbjct: 297 GHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of CSPI05G28520 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 162.2 bits (409), Expect = 6.6e-40
Identity = 97/262 (37.02%), Postives = 137/262 (52.29%), Query Frame = 0


Query: 45  IHNIKKYLQRYGYLSHNMTTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETL 104
           I  IK++LQ+YGYL  N  +D           D S E A+  YQK   L ++G  D +TL
Sbjct: 50  IPEIKRHLQQYGYLPQNKESD-----------DVSFEQALVRYQKNLGLPITGKPDSDTL 109

Query: 105 DQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWP-DYKRHLTYVFTNN---- 164
            Q+  PRCG  D  +  ++            Y  FPG  +W  D    LTY F+      
Sbjct: 110 SQILLPRCGFPDDVEPKTA-----PFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTP 169

Query: 165 --FPINFVPSVTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVG 224
              P +       A  +WA+    +F E  D   ADI I F   DH DG PFDG  GV+ 
Sbjct: 170 YLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLA 229

Query: 225 HAFAPIDGRLHLDGDDSWSAGLEEKK----VNVMNAALHELGHVLGLAHSTLPQAVMWPY 284
           H F+P +GRLHLD  ++W+   +E+K    V++ + A+HE+GHVLGL HS++  A M+P 
Sbjct: 230 HTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPT 289

Query: 285 IESNALK-NLNDDDIAGLHALY 295
           ++  + K NLN DD+ G+ +LY
Sbjct: 290 LKPRSKKVNLNMDDVVGVQSLY 295

The following BLAST results are available for this feature:

BLAST of CSPI05G28520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZUJ5	9.6e-44	38.18	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
O04529	1.3e-43	37.15	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
P29136	1.3e-43	39.16	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
Q5XF51	4.0e-42	34.47	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
O23507	1.5e-41	36.73	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]

BLAST of CSPI05G28520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KNY8	9.3e-159	92.20	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G522910 PE=3 S...	[more]
A0A0A0KX71	1.4e-154	90.17	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G631560 PE=3 S...	[more]
A0A0A0KVD8	1.0e-149	89.04	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633150 PE=3 S...	[more]
A0A5A7T3V3	4.8e-131	78.38	Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C9J6	4.8e-131	78.38	metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498179 PE=3 SV...	[more]

BLAST of CSPI05G28520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031741767.1	1.5e-166	98.64	metalloendoproteinase 5-MMP-like [Cucumis sativus] >KAE8648903.1 hypothetical pr...	[more]
XP_004142458.3	6.6e-159	92.88	metalloendoproteinase 1 [Cucumis sativus] >KAE8648904.1 hypothetical protein Csa...	[more]
XP_004142843.3	1.5e-158	92.20	metalloendoproteinase 1 [Cucumis sativus] >KAE8648550.1 hypothetical protein Csa...	[more]
XP_004142460.2	2.9e-154	90.17	metalloendoproteinase 1 [Cucumis sativus] >KGN52406.1 hypothetical protein Csa_0...	[more]
XP_011656276.2	2.5e-150	89.38	metalloendoproteinase 1 [Cucumis sativus] >KAE8648905.1 hypothetical protein Csa...	[more]

BLAST of CSPI05G28520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G59970.1	6.8e-45	38.18	Matrixin family protein	[more]
AT1G70170.1	8.9e-45	37.15	matrix metalloproteinase	[more]
AT1G24140.1	2.9e-43	34.47	Matrixin family protein	[more]
AT4G16640.1	1.1e-42	36.73	Matrixin family protein	[more]
AT2G45040.1	6.6e-40	37.02	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 249..274 score: 53.99 coord: 168..183 score: 24.7 coord: 107..120 score: 55.14 coord: 192..220 score: 54.67 coord: 282..295 score: 46.78
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 141..295 e-value: 7.1E-23 score: 92.0
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 151..294 e-value: 3.4E-37 score: 127.7
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 43..107 e-value: 2.3E-11 score: 43.7
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 36..295 e-value: 1.3E-68 score: 233.1
None	No IPR available	PIRSR	PIRSR001191-1	PIRSR001191-1	coord: 46..294 e-value: 1.5E-53 score: 180.0
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 25..294
None	No IPR available	PANTHER	PTHR10201:SF272	METALLOENDOPROTEINASE 2-MMP-LIKE	coord: 25..294
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 138..295
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 144..294 e-value: 1.62085E-49 score: 159.677
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 38..117

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G28520.1	CSPI05G28520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity