CSPI05G28510 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G28510
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionmetalloendoproteinase 2-MMP-like
LocationChr5: 26890085 .. 26890810 (-)
RNA-Seq ExpressionCSPI05G28510
SyntenyCSPI05G28510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGTACATTTTATCTTCTATCCATATATAATTTTACGTGCATTTTTCATCTATATTACAACAATTTGGACTTTTTATGACAGAAAAATAAAGACAAATTAGGAGAGAGAAAAGTCTGCAATAAAACTTACAAAGAAAATAAACATTTGAACTATATTTTTCTATGCTCAACAGGTGGCACATTCGAGAGAGTTATTTTGAAACGATAA

mRNA sequence

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGTGGCACATTCGAGAGAGTTATTTTGAAACGATAA

Coding sequence (CDS)

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGTGGCACATTCGAGAGAGTTATTTTGAAACGATAA

Protein sequence

MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGGTFERVILKR*
Homology
BLAST of CSPI05G28510 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.4e-24
Identity = 56/133 (42.11%), Postives = 81/133 (60.90%), Query Frame = 0

Query: 42  NLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNL 101
           N  GC  G K +G++ +K+Y QHFGY+     N S +F DD FD +L++A++ YQ N+ L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITE--TNLSGNFTDD-FDDILKNAVEMYQRNFQL 107

Query: 102 KATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKING-HFHSVSHYAFFDG 161
             TG ++  TL  +  PRCG  D+V+G + M SG+K         G  FH+V HY+FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 162 NPKWPASKSHLTY 174
            P+WP ++  LTY
Sbjct: 168 EPRWPRNRRDLTY 177

BLAST of CSPI05G28510 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 9.0e-24
Identity = 55/152 (36.18%), Postives = 88/152 (57.89%), Query Frame = 0

Query: 25  SSSHPKSHLSNGLVF---LKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDD 84
           S++ P S  +   VF     N  GC  G  V+G++++K+Y Q FGY+    + +S +F D
Sbjct: 26  STAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYI---PETFSGNFTD 85

Query: 85  DEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQ 144
           D FD +L++A++ YQ N+NL  TG ++A T+  +  PRCG  D+V+G + M  G++   +
Sbjct: 86  D-FDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFE 145

Query: 145 HRKINGHFHSVSHYAFFDGNPKWPASKSHLTY 174
                 H H+V  Y  F G P+WP ++  LTY
Sbjct: 146 VNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTY 173

BLAST of CSPI05G28510 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.5e-20
Identity = 59/172 (34.30%), Postives = 88/172 (51.16%), Query Frame = 0

Query: 5   TLLSFLTTSFFLVIVLFPLISSSHPKSHLS---NGLVFLKNLNGCKKGDKVEGIHQVKEY 64
           T+L F  T   +    +  +SS  P   L+   N       L GC  G+ + G+ ++K+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 65  LQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCG 124
            + FGY+       +     D+FD VL+SAI TYQ N+NLK TG +++ TL  + +PRCG
Sbjct: 67  FRRFGYIT------TTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 126

Query: 125 FADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTY 174
             D++DG + M  GK +            +   Y+FF G P+WP  K  LTY
Sbjct: 127 NPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTY 161

BLAST of CSPI05G28510 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 5.3e-16
Identity = 50/126 (39.68%), Postives = 67/126 (53.17%), Query Frame = 0

Query: 49  GDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTIN 108
           G  V G+ ++K YL  FGY+N+  + +S     D FDG LESAI  YQ N  L  TG ++
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS-----DVFDGPLESAISLYQENLGLPITGRLD 123

Query: 109 AKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHF-HSVSHYAFFDGNPKWPAS 168
             T+ LMS PRCG +D                 H  IN  F H+ +HY +F+G PKW  +
Sbjct: 124 TSTVTLMSLPRCGVSD----------------THMTINNDFLHTTAHYTYFNGKPKW--N 166

Query: 169 KSHLTY 174
           +  LTY
Sbjct: 184 RDTLTY 166

BLAST of CSPI05G28510 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 1.0e-14
Identity = 60/170 (35.29%), Postives = 78/170 (45.88%), Query Frame = 0

Query: 4   ETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQ 63
           + LL  L T +FL   L P +S+  P  +  +G    K       G   +G+  VK Y  
Sbjct: 7   QELLVALATLYFLATSL-PSVSAHGP--YAWDGEATYK-FTTYHPGQNYKGLSNVKNYFH 66

Query: 64  HFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFA 123
           H GY+ N     +  FDD+ FD  L SAIKTYQ NYNL  TG  +  TL  +  PRCG  
Sbjct: 67  HLGYIPN-----APHFDDN-FDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVP 126

Query: 124 DIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTY 174
           DI+    +  S              F  +S Y FF   P+W A  + LTY
Sbjct: 127 DIIINTNKTTS--------------FGMISDYTFFKDMPRWQAGTTQLTY 152

BLAST of CSPI05G28510 vs. ExPASy TrEMBL
Match: A0A0A0KU08 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632130 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.7e-94
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC
Sbjct: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG
Sbjct: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 174

BLAST of CSPI05G28510 vs. ExPASy TrEMBL
Match: A0A5D3BMC5 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00180 PE=3 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 7.7e-87
Identity = 158/174 (90.80%), Postives = 166/174 (95.40%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M FE LLSFLTTSFFLVIVLFPLISSSHP  H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFEKLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGT++AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           G ADI+DGKTRMKSGKKMVNQHRKINGHFH+VSHYAFFDGNPKWPASKSHLTYG
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKSHLTYG 174

BLAST of CSPI05G28510 vs. ExPASy TrEMBL
Match: A0A1S3C9I6 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 7.7e-87
Identity = 158/174 (90.80%), Postives = 166/174 (95.40%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M FETLLSFLTTSFFLVIVLFPLISSSHP  H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGT++AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           G ADI+DGKTRMKSGKKMVNQHRKINGHFH+VSHYAFFDGNPKWPASK HLTYG
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYG 174

BLAST of CSPI05G28510 vs. ExPASy TrEMBL
Match: A0A6J1FDT9 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.2e-63
Identity = 127/174 (72.99%), Postives = 146/174 (83.91%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MA    LSFL +S FL+I LFPLISSSH     S+GL+FL NLNGCKKGD VEG+HQ+K 
Sbjct: 1   MAVLQALSFLASSLFLLIALFPLISSSHQYPAQSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YL+ FGYLN  VQN+SK++ DDEFD VLESAI+TYQLNYNL+ATGT++A TL LMS+PRC
Sbjct: 61  YLRRFGYLNR-VQNHSKTY-DDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           G AD+  GKTRM SGKKMVN+HRKI+G FH VSH+AFFDGNPKWPASKSHLTYG
Sbjct: 121 GVADVFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYG 172

BLAST of CSPI05G28510 vs. ExPASy TrEMBL
Match: A0A0A0KS47 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 3.6e-60
Identity = 121/174 (69.54%), Postives = 141/174 (81.03%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M F+ L  F T + FL+I LFPLISSS PK    +GLVFLKNL GCKKGDKVEGIHQ+K+
Sbjct: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKP--VDGLVFLKNLKGCKKGDKVEGIHQMKK 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNN V   S+S  DDEFD +LE A+KTYQ+NYNLK TGT++  TL  MS+PRC
Sbjct: 61  YLQHFGYLNNDVHIRSES-SDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           G ADI++GKT MKSGK+ VNQHRKI+GHFH VSH+AFF+GN +WP SKSHLTYG
Sbjct: 121 GVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYG 171

BLAST of CSPI05G28510 vs. NCBI nr
Match: XP_004142461.1 (metalloendoproteinase 2-MMP [Cucumis sativus] >KGN52414.1 hypothetical protein Csa_009393 [Cucumis sativus])

HSP 1 Score: 355.1 bits (910), Expect = 3.6e-94
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC
Sbjct: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG
Sbjct: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 174

BLAST of CSPI05G28510 vs. NCBI nr
Match: XP_008458907.1 (PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo])

HSP 1 Score: 329.7 bits (844), Expect = 1.6e-86
Identity = 158/174 (90.80%), Postives = 166/174 (95.40%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M FETLLSFLTTSFFLVIVLFPLISSSHP  H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGT++AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           G ADI+DGKTRMKSGKKMVNQHRKINGHFH+VSHYAFFDGNPKWPASK HLTYG
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYG 174

BLAST of CSPI05G28510 vs. NCBI nr
Match: KAA0037435.1 (metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK00225.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 329.7 bits (844), Expect = 1.6e-86
Identity = 158/174 (90.80%), Postives = 166/174 (95.40%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M FE LLSFLTTSFFLVIVLFPLISSSHP  H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFEKLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGT++AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           G ADI+DGKTRMKSGKKMVNQHRKINGHFH+VSHYAFFDGNPKWPASKSHLTYG
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKSHLTYG 174

BLAST of CSPI05G28510 vs. NCBI nr
Match: XP_038889209.1 (metalloendoproteinase 2-MMP-like [Benincasa hispida])

HSP 1 Score: 287.0 bits (733), Expect = 1.2e-73
Identity = 143/174 (82.18%), Postives = 157/174 (90.23%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MAFE  LSF  TSFFL+I++FPLISSS P +  S+GLVFL NL+GCKKGDKVEGIHQ+K 
Sbjct: 1   MAFEG-LSFFITSFFLLIIIFPLISSSEPNNAHSHGLVFLNNLHGCKKGDKVEGIHQMKA 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYL N+VQN+SKSF DDEFD VLESAIKTYQLNYNLKATGT++A TL LMS+PRC
Sbjct: 61  YLQHFGYL-NHVQNHSKSFSDDEFDEVLESAIKTYQLNYNLKATGTLDATTLALMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           G AD+VDGKT MKSGKKMVNQHRKINGHFHSVSHYAFF+GNPKWPASKSHLTYG
Sbjct: 121 GIADVVDGKTMMKSGKKMVNQHRKINGHFHSVSHYAFFEGNPKWPASKSHLTYG 172

BLAST of CSPI05G28510 vs. NCBI nr
Match: XP_022938357.1 (metalloendoproteinase 2-MMP-like [Cucurbita moschata])

HSP 1 Score: 252.7 bits (644), Expect = 2.5e-63
Identity = 127/174 (72.99%), Postives = 146/174 (83.91%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MA    LSFL +S FL+I LFPLISSSH     S+GL+FL NLNGCKKGD VEG+HQ+K 
Sbjct: 1   MAVLQALSFLASSLFLLIALFPLISSSHQYPAQSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YL+ FGYLN  VQN+SK++ DDEFD VLESAI+TYQLNYNL+ATGT++A TL LMS+PRC
Sbjct: 61  YLRRFGYLNR-VQNHSKTY-DDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           G AD+  GKTRM SGKKMVN+HRKI+G FH VSH+AFFDGNPKWPASKSHLTYG
Sbjct: 121 GVADVFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYG 172

BLAST of CSPI05G28510 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 114.4 bits (285), Expect = 9.8e-26
Identity = 56/133 (42.11%), Postives = 81/133 (60.90%), Query Frame = 0

Query: 42  NLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNL 101
           N  GC  G K +G++ +K+Y QHFGY+     N S +F DD FD +L++A++ YQ N+ L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITE--TNLSGNFTDD-FDDILKNAVEMYQRNFQL 107

Query: 102 KATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKING-HFHSVSHYAFFDG 161
             TG ++  TL  +  PRCG  D+V+G + M SG+K         G  FH+V HY+FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 162 NPKWPASKSHLTY 174
            P+WP ++  LTY
Sbjct: 168 EPRWPRNRRDLTY 177

BLAST of CSPI05G28510 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 111.7 bits (278), Expect = 6.4e-25
Identity = 55/152 (36.18%), Postives = 88/152 (57.89%), Query Frame = 0

Query: 25  SSSHPKSHLSNGLVF---LKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDD 84
           S++ P S  +   VF     N  GC  G  V+G++++K+Y Q FGY+    + +S +F D
Sbjct: 26  STAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYI---PETFSGNFTD 85

Query: 85  DEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQ 144
           D FD +L++A++ YQ N+NL  TG ++A T+  +  PRCG  D+V+G + M  G++   +
Sbjct: 86  D-FDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFE 145

Query: 145 HRKINGHFHSVSHYAFFDGNPKWPASKSHLTY 174
                 H H+V  Y  F G P+WP ++  LTY
Sbjct: 146 VNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTY 173

BLAST of CSPI05G28510 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.5e-21
Identity = 59/172 (34.30%), Postives = 88/172 (51.16%), Query Frame = 0

Query: 5   TLLSFLTTSFFLVIVLFPLISSSHPKSHLS---NGLVFLKNLNGCKKGDKVEGIHQVKEY 64
           T+L F  T   +    +  +SS  P   L+   N       L GC  G+ + G+ ++K+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 65  LQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCG 124
            + FGY+       +     D+FD VL+SAI TYQ N+NLK TG +++ TL  + +PRCG
Sbjct: 67  FRRFGYIT------TTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 126

Query: 125 FADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTY 174
             D++DG + M  GK +            +   Y+FF G P+WP  K  LTY
Sbjct: 127 NPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTY 161

BLAST of CSPI05G28510 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 85.9 bits (211), Expect = 3.7e-17
Identity = 50/126 (39.68%), Postives = 67/126 (53.17%), Query Frame = 0

Query: 49  GDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTIN 108
           G  V G+ ++K YL  FGY+N+  + +S     D FDG LESAI  YQ N  L  TG ++
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS-----DVFDGPLESAISLYQENLGLPITGRLD 123

Query: 109 AKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHF-HSVSHYAFFDGNPKWPAS 168
             T+ LMS PRCG +D                 H  IN  F H+ +HY +F+G PKW  +
Sbjct: 124 TSTVTLMSLPRCGVSD----------------THMTINNDFLHTTAHYTYFNGKPKW--N 166

Query: 169 KSHLTY 174
           +  LTY
Sbjct: 184 RDTLTY 166

BLAST of CSPI05G28510 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 56.2 bits (134), Expect = 3.2e-08
Identity = 37/110 (33.64%), Postives = 50/110 (45.45%), Query Frame = 0

Query: 55  IHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDL 114
           I ++K +LQ +GYL       +K  DD  F    E A+  YQ N  L  TG  ++ TL  
Sbjct: 50  IPEIKRHLQQYGYLPQ-----NKESDDVSF----EQALVRYQKNLGLPITGKPDSDTLSQ 109

Query: 115 MSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKW 165
           +  PRCGF D V+ KT                  FH+   Y +F G P+W
Sbjct: 110 ILLPRCGFPDDVEPKT----------------APFHTGKKYVYFPGRPRW 134

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5XF511.4e-2442.11Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
O045299.0e-2436.18Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
Q9ZUJ53.5e-2034.30Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
O235075.3e-1639.68Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
P291361.0e-1435.29Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KU081.7e-94100.00ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632130 PE=3 S... [more]
A0A5D3BMC57.7e-8790.80Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C9I67.7e-8790.80metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV... [more]
A0A6J1FDT91.2e-6372.99metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 P... [more]
A0A0A0KS473.6e-6069.54ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_004142461.13.6e-94100.00metalloendoproteinase 2-MMP [Cucumis sativus] >KGN52414.1 hypothetical protein C... [more]
XP_008458907.11.6e-8690.80PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo][more]
KAA0037435.11.6e-8690.80metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK00225.1 metalloe... [more]
XP_038889209.11.2e-7382.18metalloendoproteinase 2-MMP-like [Benincasa hispida][more]
XP_022938357.12.5e-6372.99metalloendoproteinase 2-MMP-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G24140.19.8e-2642.11Matrixin family protein [more]
AT1G70170.16.4e-2536.18matrix metalloproteinase [more]
AT1G59970.12.5e-2134.30Matrixin family protein [more]
AT4G16640.13.7e-1739.68Matrixin family protein [more]
AT2G45040.13.2e-0833.64Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 53..115
e-value: 9.0E-11
score: 41.8
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 42..178
e-value: 4.9E-22
score: 80.4
NoneNo IPR availablePANTHERPTHR10201:SF269METALLOENDOPROTEINASE 3-MMP-LIKEcoord: 14..175
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 14..175
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 46..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G28510.1CSPI05G28510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity