CSPI05G28500 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G28500
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	metalloendoproteinase 2-MMP-like
Location	Chr5: 26887183 .. 26888465 (-)
RNA-Seq Expression	CSPI05G28500
Synteny	CSPI05G28500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGTACATTTTATCTTCTATCCATATATAATTTTACGTGCATTTTTCATCTATATTACAACAATTTGGACTTTTTATGACAGAAAAATAAAGACAAATTAGGAGAGAGAAAAGTCTGCAATAAAACTTAAATTGACTTTTTTTCTGTGAAAAAAACAATTCGTTATCAAAATTTTCAAAATTTACTAAAAATTTTTATGTCACAAATTTACGATGGTATAAATAACTATCATTAAAGTTGGATTATAACAAGCTAGATTTTCTATTTTGATAGCACGTTAATTTGATTGTTGAATTTAAACTTATTCTAAATCTTTTATTCACATTCTTAACAGGATTTCTTCCGGGAACCCCATCGAAGGCAGTAGCTACGATAGGTCGAGCATTTAAGACATGGGGTGCAAACACTCACTTCAACTTCTCCCAAACATCTCGATACAAAAAAGCCGACATCAAAATAAGTTTTGAAACTGGCGACCATGGAGATGGCCATTCCTTCGATGGCGTTCGAGGAGTTATAGCTCATGGTTTTGCACCAAGAGATGGGAGATTACATTTCGACGCAGCCGAGTCATGGGCCGTCGGTGCTATTGCTGATTCGTTTGACTTGGAGACCATAGCTTTACATGAGATCGGACACCTTCTCGGCCTCTATCACAGCTCCATCGAAAGGGCCATTATGTGGCCAAGCATCATGGAAGGATCTACCAAGGGTTTGGATGCCGATGACATTGCAGGCATTAATGCATTATACAATGCTAATTGA

mRNA sequence

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGATTTCTTCCGGGAACCCCATCGAAGGCAGTAGCTACGATAGGTCGAGCATTTAAGACATGGGGTGCAAACACTCACTTCAACTTCTCCCAAACATCTCGATACAAAAAAGCCGACATCAAAATAAGTTTTGAAACTGGCGACCATGGAGATGGCCATTCCTTCGATGGCGTTCGAGGAGTTATAGCTCATGGTTTTGCACCAAGAGATGGGAGATTACATTTCGACGCAGCCGAGTCATGGGCCGTCGGTGCTATTGCTGATTCGTTTGACTTGGAGACCATAGCTTTACATGAGATCGGACACCTTCTCGGCCTCTATCACAGCTCCATCGAAAGGGCCATTATGTGGCCAAGCATCATGGAAGGATCTACCAAGGGTTTGGATGCCGATGACATTGCAGGCATTAATGCATTATACAATGCTAATTGA

Coding sequence (CDS)

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGATTTCTTCCGGGAACCCCATCGAAGGCAGTAGCTACGATAGGTCGAGCATTTAAGACATGGGGTGCAAACACTCACTTCAACTTCTCCCAAACATCTCGATACAAAAAAGCCGACATCAAAATAAGTTTTGAAACTGGCGACCATGGAGATGGCCATTCCTTCGATGGCGTTCGAGGAGTTATAGCTCATGGTTTTGCACCAAGAGATGGGAGATTACATTTCGACGCAGCCGAGTCATGGGCCGTCGGTGCTATTGCTGATTCGTTTGACTTGGAGACCATAGCTTTACATGAGATCGGACACCTTCTCGGCCTCTATCACAGCTCCATCGAAAGGGCCATTATGTGGCCAAGCATCATGGAAGGATCTACCAAGGGTTTGGATGCCGATGACATTGCAGGCATTAATGCATTATACAATGCTAATTGA

Protein sequence

MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKGLDADDIAGINALYNAN*

Homology

[Close]

BLAST of CSPI05G28500 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 3.4e-63
Identity = 127/305 (41.64%), Postives = 178/305 (58.36%), Query Frame = 0


Query: 25  SSSHPKSHLSNGLVF---LKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDD 84
           S++ P S  +   VF     N  GC  G  V+G++++K+Y Q FGY+    + +S +F D
Sbjct: 26  STAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYI---PETFSGNFTD 85

Query: 85  DEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQ 144
           D FD +L++A++ YQ N+NL  TG ++A T+  +  PRCG  D+V+G + M  G++   +
Sbjct: 86  D-FDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFE 145

Query: 145 HRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP--SKAVATIGRAFKTWGANTH 204
                 H H+V  Y  F G P+WP ++  LTY F P  P   +  +   RAF  W   T 
Sbjct: 146 VNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTA 205

Query: 205 FNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVGAI 264
            NF+ +  +  +DI I F TGDHGDG  FDGV G +AH F+P  G+ H DA E+W V   
Sbjct: 206 LNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGD 265

Query: 265 ADSF-------DLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINA 317
            DSF       DLE++A+HEIGHLLGL HSS+E +IM+P+I  G  K  L  DD+ GI  
Sbjct: 266 LDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQY 325

[Close]

BLAST of CSPI05G28500 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.3e-59
Identity = 118/287 (41.11%), Postives = 168/287 (58.54%), Query Frame = 0


Query: 42  NLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNL 101
           N  GC  G K +G++ +K+Y QHFGY+     N S +F DD FD +L++A++ YQ N+ L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITE--TNLSGNFTDD-FDDILKNAVEMYQRNFQL 107

Query: 102 KATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKING-HFHSVSHYAFFDG 161
             TG ++  TL  +  PRCG  D+V+G + M SG+K         G  FH+V HY+FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 162 NPKWPASKSHLTYGFLP--GTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFE 221
            P+WP ++  LTY F P      +  +   RAF  W   T   F++  R+  +DI I F 
Sbjct: 168 EPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFY 227

Query: 222 TGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVG--------AIADSFDLETIAL 281
           +G+HGDG  FDG    +AH F+P  G  H D  E+W V         +++++ DLE++A+
Sbjct: 228 SGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAV 287

Query: 282 HEIGHLLGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           HEIGHLLGL HSS+E +IM+P+I  G  K  L  DD+ G+  LY AN
Sbjct: 288 HEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGAN 331

[Close]

BLAST of CSPI05G28500 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.4e-54
Identity = 124/327 (37.92%), Postives = 170/327 (51.99%), Query Frame = 0


Query: 5   TLLSFLTTSFFLVIVLFPLISSSHPKSHLS---NGLVFLKNLNGCKKGDKVEGIHQVKEY 64
           T+L F  T   +    +  +SS  P   L+   N       L GC  G+ + G+ ++K+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 65  LQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCG 124
            + FGY+       +     D+FD VL+SAI TYQ N+NLK TG +++ TL  + +PRCG
Sbjct: 67  FRRFGYIT------TTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 126

Query: 125 FADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLP--GT 184
             D++DG + M  GK +            +   Y+FF G P+WP  K  LTY F P    
Sbjct: 127 NPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNL 186

Query: 185 PSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGF 244
             +      RAF  W   T  NF+++    +ADI I F +G+HGDG  FDG  G +AH  
Sbjct: 187 TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHAS 246

Query: 245 APRDGRLHFDAAESWAVG---------AIADSFDLETIALHEIGHLLGLYHSSIERAIMW 304
           +P  G LH D  E W +           +    DLE++A+HEIGHLLGL HSS+E AIM+
Sbjct: 247 SPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMF 306

Query: 305 PSIMEGSTK-GLDADDIAGINALYNAN 317
           P+I  G  K  L  DDI GI  LY  N
Sbjct: 307 PAISGGDRKVELAKDDIEGIQHLYGGN 316

[Close]

BLAST of CSPI05G28500 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 7.8e-52
Identity = 121/281 (43.06%), Postives = 158/281 (56.23%), Query Frame = 0


Query: 49  GDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTIN 108
           G  V G+ ++K YL  FGY+N+  + +S     D FDG LESAI  YQ N  L  TG ++
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS-----DVFDGPLESAISLYQENLGLPITGRLD 123

Query: 109 AKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHF-HSVSHYAFFDGNPKWPAS 168
             T+ LMS PRCG +D                 H  IN  F H+ +HY +F+G PKW  +
Sbjct: 124 TSTVTLMSLPRCGVSD----------------THMTINNDFLHTTAHYTYFNGKPKW--N 183

Query: 169 KSHLTYGF-----LPGTPSKAVATI-GRAFKTWGANTHFNFSQTSRYKKADIKISFETGD 228
           +  LTY       L    S+ V T+  RAF  W +    +F +   +  AD+KI F  GD
Sbjct: 184 RDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGD 243

Query: 229 HGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAV-----GAIADSFDLETIALHEIGHL 288
           HGDG  FDGV G +AH FAP +GRLH DAAE+W V     G+   + DLE++A HEIGHL
Sbjct: 244 HGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHL 303

Query: 289 LGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           LGL HSS E A+M+PS+   + K  L  DD+AG+  LY  N
Sbjct: 304 LGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPN 321

[Close]

BLAST of CSPI05G28500 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 200.3 bits (508), Expect = 3.3e-50
Identity = 129/322 (40.06%), Postives = 165/322 (51.24%), Query Frame = 0


Query: 4   ETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQ 63
           + LL  L T +FL   L P +S+  P  +  +G    K       G   +G+  VK Y  
Sbjct: 7   QELLVALATLYFLATSL-PSVSAHGP--YAWDGEATYK-FTTYHPGQNYKGLSNVKNYFH 66

Query: 64  HFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFA 123
           H GY+ N     +  FDD+ FD  L SAIKTYQ NYNL  TG  +  TL  +  PRCG  
Sbjct: 67  HLGYIPN-----APHFDDN-FDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVP 126

Query: 124 DIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFL--PGTPS 183
           DI+    +  S              F  +S Y FF   P+W A  + LTY F   P    
Sbjct: 127 DIIINTNKTTS--------------FGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDD 186

Query: 184 KAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAP 243
              + I RAF  W    +  F +T+ Y+ A+IKI F + +HGD + FDG  G++ H FAP
Sbjct: 187 TFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAP 246

Query: 244 RDGRLHFDAAESWAVGA------IADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIME 303
            DGR HFDA E W          +  +FDLE++A+HEIGHLLGL HSS  RAIM+PSI  
Sbjct: 247 TDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPP 304

Query: 304 GSTK-GLDADDIAGINALYNAN 317
            + K  L  DDI GI  LY  N
Sbjct: 307 RTRKVNLAQDDIDGIRKLYGIN 304

[Close]

BLAST of CSPI05G28500 vs. ExPASy TrEMBL
Match: A0A0A0KU08 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632130 PE=3 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 1.9e-181
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC
Sbjct: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP
Sbjct: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA
Sbjct: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG
Sbjct: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

[Close]

BLAST of CSPI05G28500 vs. ExPASy TrEMBL
Match: A0A5D3BMC5 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00180 PE=3 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 8.4e-166
Identity = 283/316 (89.56%), Postives = 301/316 (95.25%), Query Frame = 0


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M FE LLSFLTTSFFLVIVLFPLISSSHP  H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFEKLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGT++AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G ADI+DGKTRMKSGKKMVNQHRKINGHFH+VSHYAFFDGNPKWPASKSHLTYGFLPGTP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVA +GRAFKTWGA+THFNFS+ S+YKKADIKISF +GDHGDGHSFDGV GVIAHGFA
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA ESWAVGA+ADSFDLET+ALHEIGHLLGL+HSS+E AIMWPSIMEG+TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

[Close]

BLAST of CSPI05G28500 vs. ExPASy TrEMBL
Match: A0A1S3C9I6 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 8.4e-166
Identity = 283/316 (89.56%), Postives = 301/316 (95.25%), Query Frame = 0


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M FETLLSFLTTSFFLVIVLFPLISSSHP  H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGT++AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G ADI+DGKTRMKSGKKMVNQHRKINGHFH+VSHYAFFDGNPKWPASK HLTYGFLPGTP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVA +GRAFKTWGA+THFNFS+ S+YKKADIKISF +GDHGDGHSFDGV GVIAHGFA
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA ESWAVGA+ADSFDLET+ALHEIGHLLGL+HSS+E AIMWPSIMEG+TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

[Close]

BLAST of CSPI05G28500 vs. ExPASy TrEMBL
Match: A0A6J1FDT9 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 PE=3 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.7e-132
Identity = 234/313 (74.76%), Postives = 267/313 (85.30%), Query Frame = 0


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MA    LSFL +S FL+I LFPLISSSH     S+GL+FL NLNGCKKGD VEG+HQ+K 
Sbjct: 1   MAVLQALSFLASSLFLLIALFPLISSSHQYPAQSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YL+ FGYLN  VQN+SK++ DDEFD VLESAI+TYQLNYNL+ATGT++A TL LMS+PRC
Sbjct: 61  YLRRFGYLNR-VQNHSKTY-DDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G AD+  GKTRM SGKKMVN+HRKI+G FH VSH+AFFDGNPKWPASKSHLTYGFLPGTP
Sbjct: 121 GVADVFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           ++A+A +GRAF TW ANTHF FS  S Y+ ADIKISFE+GDHGDG +FDGV GVIAH F+
Sbjct: 181 TEAIAPVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFS 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA E WA+GAI DSFD+ET+ALHEIGHLLGL HSS+E AIMWP+IMEG+TKG
Sbjct: 241 PTDGRLHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKG 300

Query: 301 LDADDIAGINALY 314
           L ADDIAGINALY
Sbjct: 301 LHADDIAGINALY 311

[Close]

BLAST of CSPI05G28500 vs. ExPASy TrEMBL
Match: A0A6J1JTF5 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.4e-128
Identity = 228/315 (72.38%), Postives = 266/315 (84.44%), Query Frame = 0


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MA    LSF  +S FL+I LFPLISSS      S+GL+FL NLNGCKKGD VEG+HQ+K 
Sbjct: 1   MAVLQALSFFASSLFLLITLFPLISSSPQYPAHSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YL  FGYL ++VQN+SK++ DDEFD +LESAI+TYQLNYNL+ATGT++A TL LMS+PRC
Sbjct: 61  YLCRFGYL-SHVQNHSKTY-DDEFDELLESAIRTYQLNYNLEATGTLDASTLALMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G AD+  GKT M SGKKMVN+HRKI+ HFH VSH+AFFDGNPKWPASKSHL YGFLPGTP
Sbjct: 121 GVADVFHGKTGMNSGKKMVNRHRKISRHFHGVSHFAFFDGNPKWPASKSHLAYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
            +A+A +GRAF TW ANTHF FSQ S Y+ ADIKISFE+GDHGDG +FDGV GVIAH F+
Sbjct: 181 IEAIAPVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFS 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA E+WA+GA+ +SFD+ET+ALHEIGHLLGL+HSS+E AIMWP+IMEG+TKG
Sbjct: 241 PTDGRLHFDAIETWAIGAVPNSFDMETVALHEIGHLLGLHHSSVEGAIMWPTIMEGATKG 300

Query: 301 LDADDIAGINALYNA 316
           L ADDIAGINALY A
Sbjct: 301 LHADDIAGINALYTA 313

[Close]

BLAST of CSPI05G28500 vs. NCBI nr
Match: XP_004142461.1 (metalloendoproteinase 2-MMP [Cucumis sativus] >KGN52414.1 hypothetical protein Csa_009393 [Cucumis sativus])

HSP 1 Score: 644.8 bits (1662), Expect = 3.9e-181
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC
Sbjct: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP
Sbjct: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA
Sbjct: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG
Sbjct: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

[Close]

BLAST of CSPI05G28500 vs. NCBI nr
Match: XP_008458907.1 (PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo])

HSP 1 Score: 592.8 bits (1527), Expect = 1.7e-165
Identity = 283/316 (89.56%), Postives = 301/316 (95.25%), Query Frame = 0


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M FETLLSFLTTSFFLVIVLFPLISSSHP  H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGT++AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G ADI+DGKTRMKSGKKMVNQHRKINGHFH+VSHYAFFDGNPKWPASK HLTYGFLPGTP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVA +GRAFKTWGA+THFNFS+ S+YKKADIKISF +GDHGDGHSFDGV GVIAHGFA
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA ESWAVGA+ADSFDLET+ALHEIGHLLGL+HSS+E AIMWPSIMEG+TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

[Close]

BLAST of CSPI05G28500 vs. NCBI nr
Match: KAA0037435.1 (metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK00225.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 592.8 bits (1527), Expect = 1.7e-165
Identity = 283/316 (89.56%), Postives = 301/316 (95.25%), Query Frame = 0


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M FE LLSFLTTSFFLVIVLFPLISSSHP  H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFEKLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGT++AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G ADI+DGKTRMKSGKKMVNQHRKINGHFH+VSHYAFFDGNPKWPASKSHLTYGFLPGTP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVA +GRAFKTWGA+THFNFS+ S+YKKADIKISF +GDHGDGHSFDGV GVIAHGFA
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA ESWAVGA+ADSFDLET+ALHEIGHLLGL+HSS+E AIMWPSIMEG+TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

[Close]

BLAST of CSPI05G28500 vs. NCBI nr
Match: XP_038889209.1 (metalloendoproteinase 2-MMP-like [Benincasa hispida])

HSP 1 Score: 520.8 bits (1340), Expect = 8.4e-144
Identity = 252/313 (80.51%), Postives = 282/313 (90.10%), Query Frame = 0


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MAFE  LSF  TSFFL+I++FPLISSS P +  S+GLVFL NL+GCKKGDKVEGIHQ+K 
Sbjct: 1   MAFEG-LSFFITSFFLLIIIFPLISSSEPNNAHSHGLVFLNNLHGCKKGDKVEGIHQMKA 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYL N+VQN+SKSF DDEFD VLESAIKTYQLNYNLKATGT++A TL LMS+PRC
Sbjct: 61  YLQHFGYL-NHVQNHSKSFSDDEFDEVLESAIKTYQLNYNLKATGTLDATTLALMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G AD+VDGKT MKSGKKMVNQHRKINGHFHSVSHYAFF+GNPKWPASKSHLTYGFLPGTP
Sbjct: 121 GIADVVDGKTMMKSGKKMVNQHRKINGHFHSVSHYAFFEGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           S+AVA +GRAF TW ANTHF+FSQ S Y+ ADIKISFE+GDHGDGH+FDGV GVIAH F+
Sbjct: 181 SEAVAPVGRAFTTWAANTHFSFSQASAYETADIKISFESGDHGDGHAFDGVGGVIAHAFS 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA E WA+GA+ADS+D+ET+ALHEIGHLLGL+HSS+E AIMWP+IM G+TKG
Sbjct: 241 PTDGRLHFDAVEPWAIGAVADSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMGGATKG 300

Query: 301 LDADDIAGINALY 314
           L ADDIAGINALY
Sbjct: 301 LHADDIAGINALY 311

[Close]

BLAST of CSPI05G28500 vs. NCBI nr
Match: XP_022938357.1 (metalloendoproteinase 2-MMP-like [Cucurbita moschata])

HSP 1 Score: 481.5 bits (1238), Expect = 5.6e-132
Identity = 234/313 (74.76%), Postives = 267/313 (85.30%), Query Frame = 0


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MA    LSFL +S FL+I LFPLISSSH     S+GL+FL NLNGCKKGD VEG+HQ+K 
Sbjct: 1   MAVLQALSFLASSLFLLIALFPLISSSHQYPAQSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YL+ FGYLN  VQN+SK++ DDEFD VLESAI+TYQLNYNL+ATGT++A TL LMS+PRC
Sbjct: 61  YLRRFGYLNR-VQNHSKTY-DDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G AD+  GKTRM SGKKMVN+HRKI+G FH VSH+AFFDGNPKWPASKSHLTYGFLPGTP
Sbjct: 121 GVADVFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           ++A+A +GRAF TW ANTHF FS  S Y+ ADIKISFE+GDHGDG +FDGV GVIAH F+
Sbjct: 181 TEAIAPVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFS 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA E WA+GAI DSFD+ET+ALHEIGHLLGL HSS+E AIMWP+IMEG+TKG
Sbjct: 241 PTDGRLHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKG 300

Query: 301 LDADDIAGINALY 314
           L ADDIAGINALY
Sbjct: 301 LHADDIAGINALY 311

[Close]

BLAST of CSPI05G28500 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 243.4 bits (620), Expect = 2.4e-64
Identity = 127/305 (41.64%), Postives = 178/305 (58.36%), Query Frame = 0


Query: 25  SSSHPKSHLSNGLVF---LKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDD 84
           S++ P S  +   VF     N  GC  G  V+G++++K+Y Q FGY+    + +S +F D
Sbjct: 26  STAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYI---PETFSGNFTD 85

Query: 85  DEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQ 144
           D FD +L++A++ YQ N+NL  TG ++A T+  +  PRCG  D+V+G + M  G++   +
Sbjct: 86  D-FDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFE 145

Query: 145 HRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP--SKAVATIGRAFKTWGANTH 204
                 H H+V  Y  F G P+WP ++  LTY F P  P   +  +   RAF  W   T 
Sbjct: 146 VNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTA 205

Query: 205 FNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVGAI 264
            NF+ +  +  +DI I F TGDHGDG  FDGV G +AH F+P  G+ H DA E+W V   
Sbjct: 206 LNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGD 265

Query: 265 ADSF-------DLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINA 317
            DSF       DLE++A+HEIGHLLGL HSS+E +IM+P+I  G  K  L  DD+ GI  
Sbjct: 266 LDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQY 325

[Close]

BLAST of CSPI05G28500 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.6e-60
Identity = 118/287 (41.11%), Postives = 168/287 (58.54%), Query Frame = 0


Query: 42  NLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNL 101
           N  GC  G K +G++ +K+Y QHFGY+     N S +F DD FD +L++A++ YQ N+ L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITE--TNLSGNFTDD-FDDILKNAVEMYQRNFQL 107

Query: 102 KATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKING-HFHSVSHYAFFDG 161
             TG ++  TL  +  PRCG  D+V+G + M SG+K         G  FH+V HY+FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 162 NPKWPASKSHLTYGFLP--GTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFE 221
            P+WP ++  LTY F P      +  +   RAF  W   T   F++  R+  +DI I F 
Sbjct: 168 EPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFY 227

Query: 222 TGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVG--------AIADSFDLETIAL 281
           +G+HGDG  FDG    +AH F+P  G  H D  E+W V         +++++ DLE++A+
Sbjct: 228 SGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAV 287

Query: 282 HEIGHLLGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           HEIGHLLGL HSS+E +IM+P+I  G  K  L  DD+ G+  LY AN
Sbjct: 288 HEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGAN 331

[Close]

BLAST of CSPI05G28500 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 212.2 bits (539), Expect = 5.9e-55
Identity = 124/327 (37.92%), Postives = 170/327 (51.99%), Query Frame = 0


Query: 5   TLLSFLTTSFFLVIVLFPLISSSHPKSHLS---NGLVFLKNLNGCKKGDKVEGIHQVKEY 64
           T+L F  T   +    +  +SS  P   L+   N       L GC  G+ + G+ ++K+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 65  LQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCG 124
            + FGY+       +     D+FD VL+SAI TYQ N+NLK TG +++ TL  + +PRCG
Sbjct: 67  FRRFGYIT------TTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 126

Query: 125 FADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLP--GT 184
             D++DG + M  GK +            +   Y+FF G P+WP  K  LTY F P    
Sbjct: 127 NPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNL 186

Query: 185 PSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGF 244
             +      RAF  W   T  NF+++    +ADI I F +G+HGDG  FDG  G +AH  
Sbjct: 187 TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHAS 246

Query: 245 APRDGRLHFDAAESWAVG---------AIADSFDLETIALHEIGHLLGLYHSSIERAIMW 304
           +P  G LH D  E W +           +    DLE++A+HEIGHLLGL HSS+E AIM+
Sbjct: 247 SPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMF 306

Query: 305 PSIMEGSTK-GLDADDIAGINALYNAN 317
           P+I  G  K  L  DDI GI  LY  N
Sbjct: 307 PAISGGDRKVELAKDDIEGIQHLYGGN 316

[Close]

BLAST of CSPI05G28500 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 205.7 bits (522), Expect = 5.6e-53
Identity = 121/281 (43.06%), Postives = 158/281 (56.23%), Query Frame = 0


Query: 49  GDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTIN 108
           G  V G+ ++K YL  FGY+N+  + +S     D FDG LESAI  YQ N  L  TG ++
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS-----DVFDGPLESAISLYQENLGLPITGRLD 123

Query: 109 AKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHF-HSVSHYAFFDGNPKWPAS 168
             T+ LMS PRCG +D                 H  IN  F H+ +HY +F+G PKW  +
Sbjct: 124 TSTVTLMSLPRCGVSD----------------THMTINNDFLHTTAHYTYFNGKPKW--N 183

Query: 169 KSHLTYGF-----LPGTPSKAVATI-GRAFKTWGANTHFNFSQTSRYKKADIKISFETGD 228
           +  LTY       L    S+ V T+  RAF  W +    +F +   +  AD+KI F  GD
Sbjct: 184 RDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGD 243

Query: 229 HGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAV-----GAIADSFDLETIALHEIGHL 288
           HGDG  FDGV G +AH FAP +GRLH DAAE+W V     G+   + DLE++A HEIGHL
Sbjct: 244 HGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHL 303

Query: 289 LGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           LGL HSS E A+M+PS+   + K  L  DD+AG+  LY  N
Sbjct: 304 LGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPN 321

[Close]

BLAST of CSPI05G28500 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 176.4 bits (446), Expect = 3.6e-44
Identity = 108/274 (39.42%), Postives = 144/274 (52.55%), Query Frame = 0


Query: 55  IHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDL 114
           I ++K +LQ +GYL       +K  DD  F    E A+  YQ N  L  TG  ++ TL  
Sbjct: 50  IPEIKRHLQQYGYLPQ-----NKESDDVSF----EQALVRYQKNLGLPITGKPDSDTLSQ 109

Query: 115 MSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPAS-KSHLTY 174
           +  PRCGF D V+ KT                  FH+   Y +F G P+W       LTY
Sbjct: 110 ILLPRCGFPDDVEPKT----------------APFHTGKKYVYFPGRPRWTRDVPLKLTY 169

Query: 175 GFLPG------TPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHS 234
            F          P+       RAF  W +    +F +T  Y  ADIKI F  GDHGDG  
Sbjct: 170 AFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEP 229

Query: 235 FDGVRGVIAHGFAPRDGRLHFDAAESWAVGAIAD----SFDLETIALHEIGHLLGLYHSS 294
           FDGV GV+AH F+P +GRLH D AE+WAV    +    + DLE++A+HEIGH+LGL HSS
Sbjct: 230 FDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSS 289

Query: 295 IERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           ++ A M+P++   S K  L+ DD+ G+ +LY  N
Sbjct: 290 VKDAAMYPTLKPRSKKVNLNMDDVVGVQSLYGTN 298

The following BLAST results are available for this feature:

BLAST of CSPI05G28500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04529	3.4e-63	41.64	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
Q5XF51	2.3e-59	41.11	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
Q9ZUJ5	8.4e-54	37.92	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
O23507	7.8e-52	43.06	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]
P29136	3.3e-50	40.06	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]

BLAST of CSPI05G28500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KU08	1.9e-181	100.00	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632130 PE=3 S...	[more]
A0A5D3BMC5	8.4e-166	89.56	Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C9I6	8.4e-166	89.56	metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV...	[more]
A0A6J1FDT9	2.7e-132	74.76	metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 P...	[more]
A0A6J1JTF5	2.4e-128	72.38	metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=...	[more]

BLAST of CSPI05G28500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142461.1	3.9e-181	100.00	metalloendoproteinase 2-MMP [Cucumis sativus] >KGN52414.1 hypothetical protein C...	[more]
XP_008458907.1	1.7e-165	89.56	PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo]	[more]
KAA0037435.1	1.7e-165	89.56	metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK00225.1 metalloe...	[more]
XP_038889209.1	8.4e-144	80.51	metalloendoproteinase 2-MMP-like [Benincasa hispida]	[more]
XP_022938357.1	5.6e-132	74.76	metalloendoproteinase 2-MMP-like [Cucurbita moschata]	[more]

BLAST of CSPI05G28500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70170.1	2.4e-64	41.64	matrix metalloproteinase	[more]
AT1G24140.1	1.6e-60	41.11	Matrixin family protein	[more]
AT1G59970.1	5.9e-55	37.92	Matrixin family protein	[more]
AT4G16640.1	5.6e-53	43.06	Matrixin family protein	[more]
AT2G45040.1	3.6e-44	39.42	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 187..202 score: 36.59 coord: 268..293 score: 52.11 coord: 301..314 score: 52.7 coord: 211..239 score: 53.2 coord: 115..128 score: 43.64
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 160..315 e-value: 8.7E-31 score: 118.3
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 42..316 e-value: 1.2E-73 score: 249.5
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 53..115 e-value: 2.4E-10 score: 40.4
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 164..313 e-value: 6.2E-42 score: 143.1
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 14..314
None	No IPR available	PANTHER	PTHR10201:SF269	METALLOENDOPROTEINASE 3-MMP-LIKE	coord: 14..314
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 156..316
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 163..313 e-value: 1.05713E-53 score: 171.233
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 46..125

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G28500.1	CSPI05G28500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity