CSPI05G28470 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G28470
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMetalloendoproteinase 2-MMP-like
LocationChr5: 26868988 .. 26869926 (+)
RNA-Seq ExpressionCSPI05G28470
SyntenyCSPI05G28470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTCAAATCATTACATCTCCAACTATTCCTACTTCTTACCTTCTTATTATTATTTCACCCTATTTCATCACGTGATCTCAATCACATCCATGGACCATCTCAATTTCTATTTCCCAAATATCTCATAGGAAGTCGTAAGGGTCACAACCTTGAAGGAACCCACACCATCAAAACGTACCTCCAACGTTACGGTTACTTAAGTAAAAGCTACAACATTATTGATACCAATGGCGTTTATAATAATGCCTACGATGAGCAGCTAGAATCCTCCATCAAAAAATATCAAAAGTTCTTCCAGCTTAACCAGAGTGGCATTTTAGACACAGAGACGTTACGCCAAATGTCACAGTCAAGATGTTCAGTTCCCGACTTTTTCGAGAGCGATGACAACGAAACGAGCATGACAACGAGTAATCTCCACATGGGAAGTAGGTTCAAATTTTTTCCGGGGAGACCGAAATGGCCGGATTCGAAGAATTACAGTCTTACATTCTCGTTCATTAATAATTTCCCAGGAATATTTAAGGGGGAAGTGGGGGACGCGTTTTTGGCATGGTACGAACGAAGCCGATTTTTCTTCACAGAAGTTCCCGAGGGCGAAGAGTCTGATATAAAAATAAGCTTTGAAGTAGGAGACCATGGAGATGGGCATCCTTTTCGAGTAGGAGTTTTGGCACATGCATTTACACCAACAGATGGAAGACTTCACTTCAATGGAGATGAACCATTTTCATCTGAAGTTGCAGAGGGTAAGTATCATGTGAGAAGTGTGGCACTGCACGAGCTTGGACATTCACTTGGGTTGGCGCATACAGAAATTTTAGATGCCATTATGTATCCCACCCTACCTCCTAATTTTGCTAAGAGTATAAATAGCGATGATGTTAATGGACTTTGGGCATTGTATGATAATTTTCATATTGGTGGTCCATAA

mRNA sequence

ATGGATTTCAAATCATTACATCTCCAACTATTCCTACTTCTTACCTTCTTATTATTATTTCACCCTATTTCATCACGTGATCTCAATCACATCCATGGACCATCTCAATTTCTATTTCCCAAATATCTCATAGGAAGTCGTAAGGGTCACAACCTTGAAGGAACCCACACCATCAAAACGTACCTCCAACGTTACGGTTACTTAAGTAAAAGCTACAACATTATTGATACCAATGGCGTTTATAATAATGCCTACGATGAGCAGCTAGAATCCTCCATCAAAAAATATCAAAAGTTCTTCCAGCTTAACCAGAGTGGCATTTTAGACACAGAGACGTTACGCCAAATGTCACAGTCAAGATGTTCAGTTCCCGACTTTTTCGAGAGCGATGACAACGAAACGAGCATGACAACGAGTAATCTCCACATGGGAAGTAGGTTCAAATTTTTTCCGGGGAGACCGAAATGGCCGGATTCGAAGAATTACAGTCTTACATTCTCGTTCATTAATAATTTCCCAGGAATATTTAAGGGGGAAGTGGGGGACGCGTTTTTGGCATGGTACGAACGAAGCCGATTTTTCTTCACAGAAGTTCCCGAGGGCGAAGAGTCTGATATAAAAATAAGCTTTGAAGTAGGAGACCATGGAGATGGGCATCCTTTTCGAGTAGGAGTTTTGGCACATGCATTTACACCAACAGATGGAAGACTTCACTTCAATGGAGATGAACCATTTTCATCTGAAGTTGCAGAGGGTAAGTATCATGTGAGAAGTGTGGCACTGCACGAGCTTGGACATTCACTTGGGTTGGCGCATACAGAAATTTTAGATGCCATTATGTATCCCACCCTACCTCCTAATTTTGCTAAGAGTATAAATAGCGATGATGTTAATGGACTTTGGGCATTGTATGATAATTTTCATATTGGTGGTCCATAA

Coding sequence (CDS)

ATGGATTTCAAATCATTACATCTCCAACTATTCCTACTTCTTACCTTCTTATTATTATTTCACCCTATTTCATCACGTGATCTCAATCACATCCATGGACCATCTCAATTTCTATTTCCCAAATATCTCATAGGAAGTCGTAAGGGTCACAACCTTGAAGGAACCCACACCATCAAAACGTACCTCCAACGTTACGGTTACTTAAGTAAAAGCTACAACATTATTGATACCAATGGCGTTTATAATAATGCCTACGATGAGCAGCTAGAATCCTCCATCAAAAAATATCAAAAGTTCTTCCAGCTTAACCAGAGTGGCATTTTAGACACAGAGACGTTACGCCAAATGTCACAGTCAAGATGTTCAGTTCCCGACTTTTTCGAGAGCGATGACAACGAAACGAGCATGACAACGAGTAATCTCCACATGGGAAGTAGGTTCAAATTTTTTCCGGGGAGACCGAAATGGCCGGATTCGAAGAATTACAGTCTTACATTCTCGTTCATTAATAATTTCCCAGGAATATTTAAGGGGGAAGTGGGGGACGCGTTTTTGGCATGGTACGAACGAAGCCGATTTTTCTTCACAGAAGTTCCCGAGGGCGAAGAGTCTGATATAAAAATAAGCTTTGAAGTAGGAGACCATGGAGATGGGCATCCTTTTCGAGTAGGAGTTTTGGCACATGCATTTACACCAACAGATGGAAGACTTCACTTCAATGGAGATGAACCATTTTCATCTGAAGTTGCAGAGGGTAAGTATCATGTGAGAAGTGTGGCACTGCACGAGCTTGGACATTCACTTGGGTTGGCGCATACAGAAATTTTAGATGCCATTATGTATCCCACCCTACCTCCTAATTTTGCTAAGAGTATAAATAGCGATGATGTTAATGGACTTTGGGCATTGTATGATAATTTTCATATTGGTGGTCCATAA

Protein sequence

MDFKSLHLQLFLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIKTYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQSRCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDGRLHFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNGLWALYDNFHIGGP*
Homology
BLAST of CSPI05G28470 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 8.0e-41
Identity = 117/322 (36.34%), Postives = 164/322 (50.93%), Query Frame = 0

Query: 12  LLLTFLLLF---HPISSRDLNHIHG--PSQFL---------FPKYLIGSRKGHNLEGTHT 71
           LLLT L+ F   +PIS++   ++    P QFL         F K L G   G N+ G   
Sbjct: 4   LLLTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSK-LAGCHIGENINGLSK 63

Query: 72  IKTYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMS 131
           +K Y +R+GY++ + N  D        +D+ L+S+I  YQK F L  +G LD+ TLRQ+ 
Sbjct: 64  LKQYFRRFGYITTTGNCTDD-------FDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIV 123

Query: 132 QSRCSVPDFFESDDNETSMTTSN-LHMGSRFKFFPGRPKWPDSKNYSLTFSFI--NNFPG 191
           + RC  PD     D  + M     L    ++ FFPG+P+WP  K   LT++F   NN   
Sbjct: 124 KPRCGNPDLI---DGVSEMNGGKILRTTEKYSFFPGKPRWPKRKR-DLTYAFAPQNNLTD 183

Query: 192 IFKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPF--RVGVLAHAFTP 251
             K     AF  W E +   FT       +DI I F  G+HGDG PF   +G LAHA +P
Sbjct: 184 EVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSP 243

Query: 252 TDGRLHFNGDEPF---SSEVAEGKYHV------RSVALHELGHSLGLAHTEILDAIMYPT 305
             G LH +GDE +   + E++     V       SVA+HE+GH LGL H+ + DAIM+P 
Sbjct: 244 PTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPA 303

BLAST of CSPI05G28470 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 3.7e-38
Identity = 95/281 (33.81%), Postives = 145/281 (51.60%), Query Frame = 0

Query: 45  GSRKGHNLEGTHTIKTYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQ 104
           G   G N++G + IK Y QR+GY+ +++     +G + + +D+ L+++++ YQ  F LN 
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETF-----SGNFTDDFDDILKAAVELYQTNFNLNV 108

Query: 105 SGILDTETLRQMSQSRCSVPDFFESDD---------NETSMTTSNLHMGSRFKFFPGRPK 164
           +G LD  T++ +   RC  PD                E + + ++LH   R+  FPG P+
Sbjct: 109 TGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPR 168

Query: 165 WPDSKNYSLTFSF--INNFPGIFKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVG 224
           WP ++   LT++F   N      K     AF  W + +   FT       SDI I F  G
Sbjct: 169 WPRNRR-DLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTG 228

Query: 225 DHGDGHPFR--VGVLAHAFTPTDGRLHFNGDEPF-------SSEVAEGKYHVRSVALHEL 284
           DHGDG PF   +G LAHAF+P  G+ H + DE +       S         + SVA+HE+
Sbjct: 229 DHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEI 288

Query: 285 GHSLGLAHTEILDAIMYPTLPPNFAK-SINSDDVNGLWALY 305
           GH LGL H+ + ++IMYPT+     K  + +DDV G+  LY
Sbjct: 289 GHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of CSPI05G28470 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 157.5 bits (397), Expect = 2.4e-37
Identity = 107/310 (34.52%), Postives = 156/310 (50.32%), Query Frame = 0

Query: 12  LLLTFLLLFHPISSRDLNHIHGP------SQFLFPKYLIGSRKGHNLEGTHTIKTYLQRY 71
           LL+    L+   +S      HGP      + + F  Y      G N +G   +K Y    
Sbjct: 9   LLVALATLYFLATSLPSVSAHGPYAWDGEATYKFTTY----HPGQNYKGLSNVKNYFHHL 68

Query: 72  GYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQSRCSVPD 131
           GY+  + +       +++ +D+ L S+IK YQK + LN +G  D  TL+Q+   RC VPD
Sbjct: 69  GYIPNAPH-------FDDNFDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVPD 128

Query: 132 FFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFI--NNFPGIFKGEVGDA 191
              +    T+ TTS   M S + FF   P+W  +    LT++F         FK  +  A
Sbjct: 129 IIIN----TNKTTS-FGMISDYTFFKDMPRW-QAGTTQLTYAFSPEPRLDDTFKSAIARA 188

Query: 192 FLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPF--RVGVLAHAFTPTDGRLHFNG 251
           F  W       F E    E ++IKI F   +HGD +PF    G+L HAF PTDGR HF+ 
Sbjct: 189 FSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDGRCHFDA 248

Query: 252 DEPF--SSEVAE----GKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAK-SINS 305
           DE +  S +V +      + + SVA+HE+GH LGL H+  L AIMYP++PP   K ++  
Sbjct: 249 DEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRKVNLAQ 301

BLAST of CSPI05G28470 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.2e-37
Identity = 97/283 (34.28%), Postives = 144/283 (50.88%), Query Frame = 0

Query: 45  GSRKGHNLEGTHTIKTYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQ 104
           G   G   +G + +K Y Q +GY++++    + +G + + +D+ L+++++ YQ+ FQLN 
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITET----NLSGNFTDDFDDILKNAVEMYQRNFQLNV 110

Query: 105 SGILDTETLRQMSQSRCSVPDFFESDDN--------ETSMT--TSNLHMGSRFKFFPGRP 164
           +G+LD  TL+ +   RC  PD               E S        H    + FFPG P
Sbjct: 111 TGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEP 170

Query: 165 KWPDSKNYSLTFSF--INNFPGIFKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEV 224
           +WP ++   LT++F   N      K     AF  W E +   FT V     SDI I F  
Sbjct: 171 RWPRNRR-DLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYS 230

Query: 225 GDHGDGHPF--RVGVLAHAFTPTDGRLHFNGDEPF--SSEVAEGKYHV------RSVALH 284
           G+HGDG PF   +  LAHAF+P  G  H +G+E +  S E  +G   V       SVA+H
Sbjct: 231 GEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVH 290

Query: 285 ELGHSLGLAHTEILDAIMYPTLPPNFAK-SINSDDVNGLWALY 305
           E+GH LGL H+ +  +IMYPT+     K  + +DDV G+  LY
Sbjct: 291 EIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of CSPI05G28470 vs. ExPASy Swiss-Prot
Match: Q8GWW6 (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.5e-36
Identity = 103/305 (33.77%), Postives = 149/305 (48.85%), Query Frame = 0

Query: 13  LLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIKTYLQRYGYLSKSY 72
           + +F LL+  + ++ +     PSQF        +           IK +LQ+YGYL ++ 
Sbjct: 16  IFSFFLLYLNLHNQQIIEARNPSQF--------TTNPSPDVSIPEIKRHLQQYGYLPQN- 75

Query: 73  NIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQSRCSVPDFFESDDN 132
                      + D   E ++ +YQK   L  +G  D++TL Q+   RC  PD       
Sbjct: 76  ---------KESDDVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRCGFPD------- 135

Query: 133 ETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINN------FPGIFKGEVGDAFLA 192
           +    T+  H G ++ +FPGRP+W       LT++F          P   +     AF  
Sbjct: 136 DVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRRAFGK 195

Query: 193 WYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFR--VGVLAHAFTPTDGRLHFNGDEP 252
           W       F E  +   +DIKI F  GDHGDG PF   +GVLAH F+P +GRLH +  E 
Sbjct: 196 WASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAET 255

Query: 253 FSSEVAEGKYHV----RSVALHELGHSLGLAHTEILDAIMYPTLPPNFAK-SINSDDVNG 305
           ++ +  E K  V     SVA+HE+GH LGL H+ + DA MYPTL P   K ++N DDV G
Sbjct: 256 WAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVG 295

BLAST of CSPI05G28470 vs. ExPASy TrEMBL
Match: A0A0A0KX75 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632100 PE=3 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 8.3e-182
Identity = 312/313 (99.68%), Postives = 312/313 (99.68%), Query Frame = 0

Query: 1   MDFKSLHLQLF-LLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK 60
           MDFKSLHLQLF LLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK
Sbjct: 1   MDFKSLHLQLFLLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK 60

Query: 61  TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS 120
           TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS
Sbjct: 61  TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS 120

Query: 121 RCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGE 180
           RCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGE
Sbjct: 121 RCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGE 180

Query: 181 VGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDGRLHF 240
           VGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDGRLHF
Sbjct: 181 VGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDGRLHF 240

Query: 241 NGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNG 300
           NGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNG
Sbjct: 241 NGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNG 300

Query: 301 LWALYDNFHIGGP 313
           LWALYDNFHIGGP
Sbjct: 301 LWALYDNFHIGGP 313

BLAST of CSPI05G28470 vs. ExPASy TrEMBL
Match: A0A5D3BUL2 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00020 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 6.6e-139
Identity = 239/308 (77.60%), Postives = 269/308 (87.34%), Query Frame = 0

Query: 1   MDFKSLHLQLFLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIKT 60
           MDFKS +LQLFLLLT L LFHPISSRDLNHIHGPSQFLFPKYL+GSRKGHN+EGTH IKT
Sbjct: 1   MDFKSFYLQLFLLLTSLSLFHPISSRDLNHIHGPSQFLFPKYLLGSRKGHNVEGTHAIKT 60

Query: 61  YLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQSR 120
           YLQ YGYLSK+YNIIDTNGVYNNAYDEQLE S+KKYQKFF+LN+SGILD ETL QMSQ R
Sbjct: 61  YLQHYGYLSKNYNIIDTNGVYNNAYDEQLECSVKKYQKFFKLNESGILDRETLSQMSQPR 120

Query: 121 CSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGEV 180
           CSVPDFFES+DNETS+TTSNLH+ SR+ FFPGRPKW DS+N  LT+S I NFP  F+  +
Sbjct: 121 CSVPDFFESEDNETSITTSNLHIRSRYAFFPGRPKWTDSRN--LTYSLIKNFPEKFEAAI 180

Query: 181 GDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDGRLHFN 240
            DAF+ W+ RSRF FT V EG++SDI ISFEVGDHGDGHPF+ GVLAHAF+P DGRLHFN
Sbjct: 181 VDAFMLWFLRSRFCFTYVSEGQKSDITISFEVGDHGDGHPFKGGVLAHAFSPEDGRLHFN 240

Query: 241 GDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNGL 300
           GD+ FSS++ EGKYH+R+VA HELGHSLGLAHT I  AIMYPTLP NF+K ++ DDV+GL
Sbjct: 241 GDKSFSSDLEEGKYHIRTVAAHELGHSLGLAHTNIEAAIMYPTLPSNFSKILSQDDVDGL 300

Query: 301 WALYDNFH 309
           W LYDNFH
Sbjct: 301 WNLYDNFH 306

BLAST of CSPI05G28470 vs. ExPASy TrEMBL
Match: A0A1S3CA73 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498172 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 7.6e-135
Identity = 233/304 (76.64%), Postives = 263/304 (86.51%), Query Frame = 0

Query: 1   MDFKSLHLQLFLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIKT 60
           MDFKS +LQLFLLLT L LFHPISSRDLNHIHGPSQFLFPKYL+GSRKGHN+EGTH IKT
Sbjct: 1   MDFKSFYLQLFLLLTSLSLFHPISSRDLNHIHGPSQFLFPKYLLGSRKGHNIEGTHAIKT 60

Query: 61  YLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQSR 120
           YLQ YGYLSK+YNIIDTNGVYNNAYDEQLE S+KKYQKFF+LN+SGILD ETL QMSQ R
Sbjct: 61  YLQHYGYLSKNYNIIDTNGVYNNAYDEQLECSVKKYQKFFKLNESGILDRETLSQMSQPR 120

Query: 121 CSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGEV 180
           CSVPD FES+DNETS+TTSNLH+ SR+ FFPGRPKW DS+N  LT+S I NFP  F+  +
Sbjct: 121 CSVPDIFESEDNETSITTSNLHIRSRYAFFPGRPKWSDSRN--LTYSLIKNFPEKFEAAI 180

Query: 181 GDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDGRLHFN 240
            DAF+ W+ RSRF FT V EG++SDI ISFEVGDHGDGHPF+ GVLAHAF+P DGRLHFN
Sbjct: 181 VDAFMLWFLRSRFCFTYVSEGQKSDITISFEVGDHGDGHPFKGGVLAHAFSPEDGRLHFN 240

Query: 241 GDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNGL 300
           GD+ FSS++ EGKYH+R+VA HELGHSLGLAHT I  AIMYPTLP NF+K ++ DDV+GL
Sbjct: 241 GDKSFSSDLEEGKYHIRTVAAHELGHSLGLAHTNIEAAIMYPTLPSNFSKILSQDDVDGL 300

Query: 301 WALY 305
           W  Y
Sbjct: 301 WNFY 302

BLAST of CSPI05G28470 vs. ExPASy TrEMBL
Match: A0A0A0KS15 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632090 PE=3 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 5.7e-106
Identity = 195/313 (62.30%), Postives = 236/313 (75.40%), Query Frame = 0

Query: 1   MDFK-SLHLQLFLLLTFLLLFHP-ISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTI 60
           MDFK S  LQ+FLLL    +  P I +RDL+ I   SQ LFP++L+GS KGHN+EG HTI
Sbjct: 1   MDFKSSSDLQIFLLLLLASIAFPFIWARDLHDIK-KSQLLFPQHLLGSSKGHNIEGIHTI 60

Query: 61  KTYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQ 120
           K +LQRYGYLSK+YNIIDTNG YNNA+D+ LES+IKKYQ FF+L +SG+LD ETL QMSQ
Sbjct: 61  KMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQ 120

Query: 121 SRCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKG 180
           +RCSVPD FE+++NETS+TTSNLH+GS++ FFPGR KWPDS NY LT++ +NNFP  FK 
Sbjct: 121 ARCSVPDIFENNENETSVTTSNLHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKE 180

Query: 181 EVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVG--VLAHAFTPTDGR 240
            V  AF  WY RSRF FTEV E E  +I+ISFE G HGD HPF      LAH F P DGR
Sbjct: 181 SVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGR 240

Query: 241 LHFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDD 300
            HFN D+PFS +V    YH+R+VALHELGH+ GLAH+   DAIM+PT+P N  K ++ DD
Sbjct: 241 FHFNADKPFSVDVTYNAYHLRTVALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDD 300

Query: 301 VNGLWALYDNFHI 310
           + GLW LYD F +
Sbjct: 301 IEGLWELYDGFGV 312

BLAST of CSPI05G28470 vs. ExPASy TrEMBL
Match: A0A6J1H7I1 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460787 PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 2.2e-70
Identity = 148/308 (48.05%), Postives = 200/308 (64.94%), Query Frame = 0

Query: 1   MDFKSLHLQLFLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIKT 60
           MD +S  LQ+  +L   +  H I +R L HIH  S  +FP++L+GS KGHN+EG H ++ 
Sbjct: 1   MDLRSC-LQVMFILVAAIAPHAIMARHL-HIHKLSSSIFPQHLVGSGKGHNIEGIHNVRA 60

Query: 61  YLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQSR 120
           +LQRYGYLS +   ++T G   N +D+ LE +IK YQKFF LN SGILD ETL  MSQ R
Sbjct: 61  FLQRYGYLSTN---VETKG---NVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPR 120

Query: 121 CSVPDFFESDDNETSMTTSNLHMGSRFKFFPG-RPKWPDSKNYSLTFSFINNFPGIFKGE 180
           C VPD F ++         +  +G  + FF   +PKW  SK Y+L ++F+ +FP  +K  
Sbjct: 121 CGVPDVFINETGGDGNGDRDHPIGLHYSFFNDLKPKWSASK-YNLKYTFLGSFPEQYKNT 180

Query: 181 VGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPF--RVGVLAHAFTPTDGRL 240
           V  A ++W   S F F+E    + +D++ISFEVGDHGDG PF  + G LAHAF P DGRL
Sbjct: 181 VIQAMISWSFNSVFAFSE-GSTDNTDVRISFEVGDHGDGSPFKDKNGALAHAFAPEDGRL 240

Query: 241 HFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDV 300
           HFN    ++      K+ V SVALHELGH LGL H++I DA+M+P++PPN  K ++SDDV
Sbjct: 241 HFNAQHAWADGAYPDKFDVGSVALHELGHILGLGHSDIRDAVMWPSMPPNLKKELSSDDV 298

Query: 301 NGLWALYD 306
           +G+ ALYD
Sbjct: 301 SGISALYD 298

BLAST of CSPI05G28470 vs. NCBI nr
Match: KGN52411.1 (hypothetical protein Csa_008387 [Cucumis sativus])

HSP 1 Score: 646.0 bits (1665), Expect = 1.7e-181
Identity = 312/313 (99.68%), Postives = 312/313 (99.68%), Query Frame = 0

Query: 1   MDFKSLHLQLF-LLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK 60
           MDFKSLHLQLF LLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK
Sbjct: 1   MDFKSLHLQLFLLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK 60

Query: 61  TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS 120
           TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS
Sbjct: 61  TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS 120

Query: 121 RCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGE 180
           RCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGE
Sbjct: 121 RCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGE 180

Query: 181 VGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDGRLHF 240
           VGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDGRLHF
Sbjct: 181 VGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDGRLHF 240

Query: 241 NGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNG 300
           NGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNG
Sbjct: 241 NGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNG 300

Query: 301 LWALYDNFHIGGP 313
           LWALYDNFHIGGP
Sbjct: 301 LWALYDNFHIGGP 313

BLAST of CSPI05G28470 vs. NCBI nr
Match: KAA0037442.1 (metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK01916.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 503.4 bits (1295), Expect = 1.4e-138
Identity = 239/308 (77.60%), Postives = 269/308 (87.34%), Query Frame = 0

Query: 1   MDFKSLHLQLFLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIKT 60
           MDFKS +LQLFLLLT L LFHPISSRDLNHIHGPSQFLFPKYL+GSRKGHN+EGTH IKT
Sbjct: 1   MDFKSFYLQLFLLLTSLSLFHPISSRDLNHIHGPSQFLFPKYLLGSRKGHNVEGTHAIKT 60

Query: 61  YLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQSR 120
           YLQ YGYLSK+YNIIDTNGVYNNAYDEQLE S+KKYQKFF+LN+SGILD ETL QMSQ R
Sbjct: 61  YLQHYGYLSKNYNIIDTNGVYNNAYDEQLECSVKKYQKFFKLNESGILDRETLSQMSQPR 120

Query: 121 CSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGEV 180
           CSVPDFFES+DNETS+TTSNLH+ SR+ FFPGRPKW DS+N  LT+S I NFP  F+  +
Sbjct: 121 CSVPDFFESEDNETSITTSNLHIRSRYAFFPGRPKWTDSRN--LTYSLIKNFPEKFEAAI 180

Query: 181 GDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDGRLHFN 240
            DAF+ W+ RSRF FT V EG++SDI ISFEVGDHGDGHPF+ GVLAHAF+P DGRLHFN
Sbjct: 181 VDAFMLWFLRSRFCFTYVSEGQKSDITISFEVGDHGDGHPFKGGVLAHAFSPEDGRLHFN 240

Query: 241 GDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNGL 300
           GD+ FSS++ EGKYH+R+VA HELGHSLGLAHT I  AIMYPTLP NF+K ++ DDV+GL
Sbjct: 241 GDKSFSSDLEEGKYHIRTVAAHELGHSLGLAHTNIEAAIMYPTLPSNFSKILSQDDVDGL 300

Query: 301 WALYDNFH 309
           W LYDNFH
Sbjct: 301 WNLYDNFH 306

BLAST of CSPI05G28470 vs. NCBI nr
Match: XP_008458908.1 (PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo])

HSP 1 Score: 490.0 bits (1260), Expect = 1.6e-134
Identity = 233/304 (76.64%), Postives = 263/304 (86.51%), Query Frame = 0

Query: 1   MDFKSLHLQLFLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIKT 60
           MDFKS +LQLFLLLT L LFHPISSRDLNHIHGPSQFLFPKYL+GSRKGHN+EGTH IKT
Sbjct: 1   MDFKSFYLQLFLLLTSLSLFHPISSRDLNHIHGPSQFLFPKYLLGSRKGHNIEGTHAIKT 60

Query: 61  YLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQSR 120
           YLQ YGYLSK+YNIIDTNGVYNNAYDEQLE S+KKYQKFF+LN+SGILD ETL QMSQ R
Sbjct: 61  YLQHYGYLSKNYNIIDTNGVYNNAYDEQLECSVKKYQKFFKLNESGILDRETLSQMSQPR 120

Query: 121 CSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGEV 180
           CSVPD FES+DNETS+TTSNLH+ SR+ FFPGRPKW DS+N  LT+S I NFP  F+  +
Sbjct: 121 CSVPDIFESEDNETSITTSNLHIRSRYAFFPGRPKWSDSRN--LTYSLIKNFPEKFEAAI 180

Query: 181 GDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDGRLHFN 240
            DAF+ W+ RSRF FT V EG++SDI ISFEVGDHGDGHPF+ GVLAHAF+P DGRLHFN
Sbjct: 181 VDAFMLWFLRSRFCFTYVSEGQKSDITISFEVGDHGDGHPFKGGVLAHAFSPEDGRLHFN 240

Query: 241 GDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNGL 300
           GD+ FSS++ EGKYH+R+VA HELGHSLGLAHT I  AIMYPTLP NF+K ++ DDV+GL
Sbjct: 241 GDKSFSSDLEEGKYHIRTVAAHELGHSLGLAHTNIEAAIMYPTLPSNFSKILSQDDVDGL 300

Query: 301 WALY 305
           W  Y
Sbjct: 301 WNFY 302

BLAST of CSPI05G28470 vs. NCBI nr
Match: XP_011656274.1 (metalloendoproteinase 4-MMP [Cucumis sativus])

HSP 1 Score: 422.2 bits (1084), Expect = 4.0e-114
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0

Query: 116 MSQSRCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGI 175
           MSQSRCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGI
Sbjct: 1   MSQSRCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGI 60

Query: 176 FKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDG 235
           FKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDG
Sbjct: 61  FKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVGVLAHAFTPTDG 120

Query: 236 RLHFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSD 295
           RLHFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSD
Sbjct: 121 RLHFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSD 180

Query: 296 DVNGLWALYDNFHIGGP 313
           DVNGLWALYDNFHIGGP
Sbjct: 181 DVNGLWALYDNFHIGGP 197

BLAST of CSPI05G28470 vs. NCBI nr
Match: XP_004142464.1 (metalloendoproteinase 2-MMP [Cucumis sativus] >KGN52410.1 hypothetical protein Csa_008522 [Cucumis sativus])

HSP 1 Score: 394.0 bits (1011), Expect = 1.2e-105
Identity = 195/313 (62.30%), Postives = 236/313 (75.40%), Query Frame = 0

Query: 1   MDFK-SLHLQLFLLLTFLLLFHP-ISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTI 60
           MDFK S  LQ+FLLL    +  P I +RDL+ I   SQ LFP++L+GS KGHN+EG HTI
Sbjct: 1   MDFKSSSDLQIFLLLLLASIAFPFIWARDLHDIK-KSQLLFPQHLLGSSKGHNIEGIHTI 60

Query: 61  KTYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQ 120
           K +LQRYGYLSK+YNIIDTNG YNNA+D+ LES+IKKYQ FF+L +SG+LD ETL QMSQ
Sbjct: 61  KMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQ 120

Query: 121 SRCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKG 180
           +RCSVPD FE+++NETS+TTSNLH+GS++ FFPGR KWPDS NY LT++ +NNFP  FK 
Sbjct: 121 ARCSVPDIFENNENETSVTTSNLHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKE 180

Query: 181 EVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVG--VLAHAFTPTDGR 240
            V  AF  WY RSRF FTEV E E  +I+ISFE G HGD HPF      LAH F P DGR
Sbjct: 181 SVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGR 240

Query: 241 LHFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDD 300
            HFN D+PFS +V    YH+R+VALHELGH+ GLAH+   DAIM+PT+P N  K ++ DD
Sbjct: 241 FHFNADKPFSVDVTYNAYHLRTVALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDD 300

Query: 301 VNGLWALYDNFHI 310
           + GLW LYD F +
Sbjct: 301 IEGLWELYDGFGV 312

BLAST of CSPI05G28470 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 169.1 bits (427), Expect = 5.7e-42
Identity = 117/322 (36.34%), Postives = 164/322 (50.93%), Query Frame = 0

Query: 12  LLLTFLLLF---HPISSRDLNHIHG--PSQFL---------FPKYLIGSRKGHNLEGTHT 71
           LLLT L+ F   +PIS++   ++    P QFL         F K L G   G N+ G   
Sbjct: 4   LLLTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSK-LAGCHIGENINGLSK 63

Query: 72  IKTYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMS 131
           +K Y +R+GY++ + N  D        +D+ L+S+I  YQK F L  +G LD+ TLRQ+ 
Sbjct: 64  LKQYFRRFGYITTTGNCTDD-------FDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIV 123

Query: 132 QSRCSVPDFFESDDNETSMTTSN-LHMGSRFKFFPGRPKWPDSKNYSLTFSFI--NNFPG 191
           + RC  PD     D  + M     L    ++ FFPG+P+WP  K   LT++F   NN   
Sbjct: 124 KPRCGNPDLI---DGVSEMNGGKILRTTEKYSFFPGKPRWPKRKR-DLTYAFAPQNNLTD 183

Query: 192 IFKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPF--RVGVLAHAFTP 251
             K     AF  W E +   FT       +DI I F  G+HGDG PF   +G LAHA +P
Sbjct: 184 EVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSP 243

Query: 252 TDGRLHFNGDEPF---SSEVAEGKYHV------RSVALHELGHSLGLAHTEILDAIMYPT 305
             G LH +GDE +   + E++     V       SVA+HE+GH LGL H+ + DAIM+P 
Sbjct: 244 PTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPA 303

BLAST of CSPI05G28470 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 160.2 bits (404), Expect = 2.6e-39
Identity = 95/281 (33.81%), Postives = 145/281 (51.60%), Query Frame = 0

Query: 45  GSRKGHNLEGTHTIKTYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQ 104
           G   G N++G + IK Y QR+GY+ +++     +G + + +D+ L+++++ YQ  F LN 
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETF-----SGNFTDDFDDILKAAVELYQTNFNLNV 108

Query: 105 SGILDTETLRQMSQSRCSVPDFFESDD---------NETSMTTSNLHMGSRFKFFPGRPK 164
           +G LD  T++ +   RC  PD                E + + ++LH   R+  FPG P+
Sbjct: 109 TGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPR 168

Query: 165 WPDSKNYSLTFSF--INNFPGIFKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVG 224
           WP ++   LT++F   N      K     AF  W + +   FT       SDI I F  G
Sbjct: 169 WPRNRR-DLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTG 228

Query: 225 DHGDGHPFR--VGVLAHAFTPTDGRLHFNGDEPF-------SSEVAEGKYHVRSVALHEL 284
           DHGDG PF   +G LAHAF+P  G+ H + DE +       S         + SVA+HE+
Sbjct: 229 DHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEI 288

Query: 285 GHSLGLAHTEILDAIMYPTLPPNFAK-SINSDDVNGLWALY 305
           GH LGL H+ + ++IMYPT+     K  + +DDV G+  LY
Sbjct: 289 GHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of CSPI05G28470 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 157.1 bits (396), Expect = 2.2e-38
Identity = 97/283 (34.28%), Postives = 144/283 (50.88%), Query Frame = 0

Query: 45  GSRKGHNLEGTHTIKTYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQ 104
           G   G   +G + +K Y Q +GY++++    + +G + + +D+ L+++++ YQ+ FQLN 
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITET----NLSGNFTDDFDDILKNAVEMYQRNFQLNV 110

Query: 105 SGILDTETLRQMSQSRCSVPDFFESDDN--------ETSMT--TSNLHMGSRFKFFPGRP 164
           +G+LD  TL+ +   RC  PD               E S        H    + FFPG P
Sbjct: 111 TGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEP 170

Query: 165 KWPDSKNYSLTFSF--INNFPGIFKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEV 224
           +WP ++   LT++F   N      K     AF  W E +   FT V     SDI I F  
Sbjct: 171 RWPRNRR-DLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYS 230

Query: 225 GDHGDGHPF--RVGVLAHAFTPTDGRLHFNGDEPF--SSEVAEGKYHV------RSVALH 284
           G+HGDG PF   +  LAHAF+P  G  H +G+E +  S E  +G   V       SVA+H
Sbjct: 231 GEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVH 290

Query: 285 ELGHSLGLAHTEILDAIMYPTLPPNFAK-SINSDDVNGLWALY 305
           E+GH LGL H+ +  +IMYPT+     K  + +DDV G+  LY
Sbjct: 291 EIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of CSPI05G28470 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 153.7 bits (387), Expect = 2.5e-37
Identity = 103/305 (33.77%), Postives = 149/305 (48.85%), Query Frame = 0

Query: 13  LLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIKTYLQRYGYLSKSY 72
           + +F LL+  + ++ +     PSQF        +           IK +LQ+YGYL ++ 
Sbjct: 16  IFSFFLLYLNLHNQQIIEARNPSQF--------TTNPSPDVSIPEIKRHLQQYGYLPQN- 75

Query: 73  NIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQSRCSVPDFFESDDN 132
                      + D   E ++ +YQK   L  +G  D++TL Q+   RC  PD       
Sbjct: 76  ---------KESDDVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRCGFPD------- 135

Query: 133 ETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINN------FPGIFKGEVGDAFLA 192
           +    T+  H G ++ +FPGRP+W       LT++F          P   +     AF  
Sbjct: 136 DVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRRAFGK 195

Query: 193 WYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFR--VGVLAHAFTPTDGRLHFNGDEP 252
           W       F E  +   +DIKI F  GDHGDG PF   +GVLAH F+P +GRLH +  E 
Sbjct: 196 WASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAET 255

Query: 253 FSSEVAEGKYHV----RSVALHELGHSLGLAHTEILDAIMYPTLPPNFAK-SINSDDVNG 305
           ++ +  E K  V     SVA+HE+GH LGL H+ + DA MYPTL P   K ++N DDV G
Sbjct: 256 WAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVG 295

BLAST of CSPI05G28470 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 153.3 bits (386), Expect = 3.2e-37
Identity = 103/282 (36.52%), Postives = 145/282 (51.42%), Query Frame = 0

Query: 43  LIGSRKGHNLEGTHTIKTYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQL 102
           L+  + G ++ G   +K YL R+GY++      D + ++++ +D  LES+I  YQ+   L
Sbjct: 58  LVDVQIGSHVSGVSELKRYLHRFGYVN------DGSEIFSDVFDGPLESAISLYQENLGL 117

Query: 103 NQSGILDTETLRQMSQSRCSVPDFFESDDNETSMTTSN--LHMGSRFKFFPGRPKW---- 162
             +G LDT T+  MS  RC V D        T MT +N  LH  + + +F G+PKW    
Sbjct: 118 PITGRLDTSTVTLMSLPRCGVSD--------THMTINNDFLHTTAHYTYFNGKPKWNRDT 177

Query: 163 ---PDSKNYSLTFSFINNFPGIFKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVG 222
                SK + L +    +   +F+     AF  W       F EV +   +D+KI F  G
Sbjct: 178 LTYAISKTHKLDYLTSEDVKTVFR----RAFSQWSSVIPVSFEEVDDFTTADLKIGFYAG 237

Query: 223 DHGDGHPFR--VGVLAHAFTPTDGRLHFNGDEPF--------SSEVAEGKYHVRSVALHE 282
           DHGDG PF   +G LAHAF P +GRLH +  E +        SSEVA     + SVA HE
Sbjct: 238 DHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVA---VDLESVATHE 297

Query: 283 LGHSLGLAHTEILDAIMYPTLPPNFAK-SINSDDVNGLWALY 305
           +GH LGL H+    A+MYP+L P   K  +  DDV G+  LY
Sbjct: 298 IGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZUJ58.0e-4136.34Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
O045293.7e-3833.81Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
P291362.4e-3734.52Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
Q5XF513.2e-3734.28Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
Q8GWW63.5e-3633.77Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KX758.3e-18299.68ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632100 PE=3 S... [more]
A0A5D3BUL26.6e-13977.60Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CA737.6e-13576.64metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498172 PE=3 SV... [more]
A0A0A0KS155.7e-10662.30ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632090 PE=3 S... [more]
A0A6J1H7I12.2e-7048.05metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460787 PE=3 ... [more]
Match NameE-valueIdentityDescription
KGN52411.11.7e-18199.68hypothetical protein Csa_008387 [Cucumis sativus][more]
KAA0037442.11.4e-13877.60metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK01916.1 metalloe... [more]
XP_008458908.11.6e-13476.64PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo][more]
XP_011656274.14.0e-114100.00metalloendoproteinase 4-MMP [Cucumis sativus][more]
XP_004142464.11.2e-10562.30metalloendoproteinase 2-MMP [Cucumis sativus] >KGN52410.1 hypothetical protein C... [more]
Match NameE-valueIdentityDescription
AT1G59970.15.7e-4236.34Matrixin family protein [more]
AT1G70170.12.6e-3933.81matrix metalloproteinase [more]
AT1G24140.12.2e-3834.28Matrixin family protein [more]
AT2G45040.12.5e-3733.77Matrixin family protein [more]
AT4G16640.13.2e-3736.52Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 204..232
score: 37.59
coord: 116..129
score: 42.86
coord: 259..284
score: 55.86
coord: 292..305
score: 38.07
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 152..306
e-value: 2.2E-20
score: 83.7
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 54..116
e-value: 4.5E-13
score: 49.2
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 46..307
e-value: 7.0E-63
score: 214.3
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 177..304
e-value: 1.8E-31
score: 109.1
NoneNo IPR availablePANTHERPTHR10201:SF272METALLOENDOPROTEINASE 2-MMP-LIKEcoord: 42..305
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 42..305
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 149..305
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 47..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G28470.1CSPI05G28470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity