CSPI05G28280 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G28280
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionWAT1-related protein
LocationChr5: 26761941 .. 26768368 (+)
RNA-Seq ExpressionCSPI05G28280
SyntenyCSPI05G28280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCGGAATTCCAACCTCTAATTAAAAATATCAATAATTAGTGGATTTGCACAATCATCTTTTATAAATAGATTTATACATAAAACCCATTGAAGTTCAAAAGAGAAGACTCTGTTTCTTCTCTAAACAAACAGAACAAGTTTTCATGGCGTTAACTGGAAGAAGCTTAATGGAAGGAGTTCTTCCATTTCCGGCGATGATGGCGATGGAGTGTATAAACGTCGGTCTCAACACACTTTTTAAAGCCGCCACTGCCGCCGGGATGAGCCACCACGTCTTCGTCGTTTACTCTTACTCTTTCGCCGCCCTTCTGCTCCTCCCTTCTCCTTTCATCTCTCGAACATCCACTCGCCTTCCACCTCTCAATTTCTCCATAACTTCAAAGATTGCTCTGCTTGGACTAATCGGGTACAGAGTAATTTCAATTGGGTTTTTGTTTGTTTGGATTTTTGAATTATTTAATTTCTGACTGTTTGAATGATCGGAAACAGAGGAATTTGAATTGGGGTTTCCGTTTGTTTGGATATGTGAATTGATGATTTGAATTTTGGTTTTTTTTTTCTTGTATTGGTAGTTCTTGTTCGCAGATCATGGGATACACAGGAATCAATTATAGTTCTCCAACACTTGCTTCCGCCATCAGCAACCTTGTTCCTGCTTTCACTTTCATTCTCGCCATCATTTTCAGGTTCTATTATTTAGTTACCAAAATACCCTTCTTCTTCTTCTTCTTCTTCCTCACCTCTTCAACTCTCCTTTTTCTCAAGGGTCATTTTTCTTTCTAGGGGTCACCTTGTTTTAATTACTTTATTTTTTGAAAAAAAAAGTTAAACTATTCTTTAAAATTATTTAAAATCAGACACATAACAAATTTTACATTTATACTAACTTTACGTTTAGTATAAATGTAACTACTGGGAATAATTTGAAATTGTTCTATATTATGTAAATATTTTAGTTTATGGTTCAAGTTGAACTTGAATAGAAAAATGTGATATTCAATAAAAAATAATAAAATTTTCACTTTTATCAAATAATATTTTAAGTTGTTTTCATTCACAAATTTTTAAAAGGAAATTAAGTTGAATTAGGTTTTTGTTTTCATTGAATTTTTGTGAATTTTGTTTTCTTTTCATTTTTAAAGAAAAAAGAAAAGAGAAAATGGTATTCTTTTGAAATTCAACTTGTTTTTTAGTTTCAAAATGTGGTTGGTTAACTAAGAATAAAGCATTTTGAAAAAAAAAATTGATGTGTTTGAAAAGGCATTCAAATAGTTTTCAAAATACTTTCAAATTTATTTTATAGTTTTTTATCTAAGAGTTTAAATATGCTGTTTTTTCTTTTCTTTTTTTTTTTTTTGGAAAAAGATGATTTTTTATTTATAAATCAAAATATGTTCTTAATCCTTTTTTAAAAAAATATTTTAAATCTTACCAATCTCAAAACTAAAATGTCAAATTTTACTTTAAAATTTTTATAAATATATATTTTACTAATATTCAATTTTGATGAGTTGTTTTGACGTTTACTTCATTTGCACAAAAACTTATTATTTTTTTTAAAAAAATGGAATTAGTTTCTCTTTACTTTTTCTCTATAATATATATAGTGAATGAGTGGATCATAAGATTAGTGGAATAAAATAATGGCTTTAATTTCTATATTTCCAAAGTCAAGTTGGATTTATAACTTTCAAGAGATTGATTTGATTGGCAATTCCCTCAAGTTTTCTTCAACTATTTTATTATTTATTATATTTTTTAGCCTACATTTTTTAAAATAGAATAGAAAATATTTAGGAGAATTTTAAAAAATGGTAGACTTTACAGTATACTTACCACTTATAGCAAATTCTATCATTTATAGTTATTAATATGTTATAGGAATTCAAAATTTTAAAAATTTTCCAAATATTTATAAAATATAGCAAAATATTATAAGATATATATTCACTTACTCAAATTTTGCTATATTTTGTAAATATTTTAAAAAGTTTTGTCATTTACTAAAATTTTCATTTTCCCACCTGTAACTCGAATATGTATTATTTCAGGTTTCTTCACCAAGATGAATTTTTGTGGTGTTTATCTATTAAAAAAACACTAATTCTCATATCAAATTTCCATCCGATATCAATATTTTCACATTTTCATATATTCAATATTGATAGTAGTATATTCACATCTTCGTTGTTATATCGTAAAAAAATTTATAAATCACAAAAGACCATTTACTAATTTAAATAATTTTTTAAAATTTAAAATTTATAATTCTAGCCTATTACGAACACCCAAACTTTTTTTTTTATACTCTATCAAAAATTTCTTAAAATTGAGACATTAACATATATAACTATATTTGTAAATTTTTATCTTAAACTTATTCTACATTAATATTATTTTCACAAAAAGAAAATTACTCAAATTAGTAAATGATTTTTTTGTTAAAACTTCTTGTGTATATTATTTAATGATAAGAATGACTTTATTTTTCTGTCTTGTTGGGTGGGCTATGTATAATGTAAGTGTGACAAAAGCAAATGTCTTTTTCCTTAGGCTTACAATTATTTACACATCCATTTTTAGAGCACCACAAAATTAAAGTAATTCATATTAAATAAAAGGCTTACTTTTAAATTCATCTCACTTTATTAAAACAACCTAACATCTTCAAAAAAAAAAAAAATCAAAATGTAGCAAGGGACATTCCAACACAAACTAGTTTCACAATTTTTTGTTTCATTATTTCTTTTTATTAAACATGCTTATTTACTTAAAAATGAATTTTTTAAAAAAATATATCACAATCAAATTGAGCACGCAAATCACATATAAACTCTATATTTTATAAATATTTTGATTTATTTAGCTATATTTTAAAATAATTTAATATTTGTTTTAGTTATAAAAAGCTTTCTTTTATTATTTTTTTGTTGGGTTGATCATACATGCAAGTGTGTATCATTGTTTTTGTTGAATGATATGATGCATTGATCAAAATTTTCACAAGAATTCTCATAATGAATGTGATTGTGACAAGATTCTTGCTTCTTTTATTGTCAAATTATCTTTTTCCTTTTGAAAATAAAATGTCAAAATCAAACATTAAAAAATCCATTTAAATTTGGTGTCCTTTTCTTTTGTAATTAAGTGTAGAAGGAGCATGTGTAACACACGTCTCCTCATGACTATAAAAGCTTAGGTTTAACCCTTATTTCTCACTTCATAAATAAGAAAAAATGAGATCATTGGATTCACTCAATCCAACTCCAAACTACGAAAGCTTAGGTTTAAGTTTAACAAAATTTGGGTCAAGTTTGTATCGTGGTTAGCTTCCCTCATACAATACAAATTATTCTATAATTTTTTAAAAAGGAATAGTTGCAAATATAACAGCTAAATTCAAAATAATTAAATATATTACAACATTTTAAAAAAATTACAAATATAGCAACATTTCTCAAAGTATATCAATAATAGAACATGTTACAAATATTGATTAATTACTCCTAGACCATAAGAATCTATCCGTGTAACTGATAAATTTTGTTATATTTATAATTTTTTAAAACATTATTATATATTTAATTATTATTCCTAAAATTGTCATAAATTATAATTACTCTTTCGAATATATATGTATATATGTATAATTGAATCTCAAATAAAAAATGTATTTTCTGCCATCCATAATATATGTGATAGTGAAGGAAGACGTAAAATATCTAAATCAAAGAATTCAAATTACATTAATATTAAATAATCATGGACGATAACTTTGCTATCTTTTATAAATGTTCGTAAAACATATCCATATCTTATTAGTTTGATTGTAAAAGCAAGTAAGTGGTTGTTAACCTAAACAAATATCCTAAATCTTTTAAGTTACATAAATATGTGTGGATATATCATAATTTAAAATTGTGATATTGTGTGATGTTACTTTGTTATCCTTGAAGTTGATGAATGCAATTTAGTGAGAAATGTGTGTATGTTGATATTATTAAAATTAGGATGGAGAAAGTGGTATTGAAAAGCAATAGCAGTCAAGCAAAAGTGATGGGCACAATAATATCAATATCAGGGGCATTTGTGGTAACATTTTACAAGGGTCCAAGAATCATATTCTCATCTTCACCAACCATTTCTCTTTCACTTCATCACAATTCACAACAACTATCCTCTTCTGATTCTAATTGGGTTATTGGTAGCCTTCTCTTGACTGCTGAATATATCCTTGTTCCACTTTGGTACATTGTTCAGGTAATCAAATCTCATTTTCTAATCCCTATTATACACTCTAATCTTTTACTATTCCTCTTCTTTTCCTTTTAGGTCTAATTATCACTAATGCCCTCTTCCCATGAACCCAACATTTACTTTACTCTCACAAAACTGCCTCTAAGTGTGAGGGGTTTATTATGTTTCTTTGCAGTCCTAATAGGTAAAATCTATATTTGGTTTGGCTCCCACATGCAAATCTACTCATCTTTCTAGTTCCTCGTGATAAGGAATATCGACCTCCTCCATATATCGCCACAATGGTGTGATATTGTCCACTTAGAGGTGTTTGAGACGTTGAGTTGAGTTTTGAAGTATGGAATTTGGAAGTTTGTGTTTAGGGTGTAGAGTTGTTTTGGCTGAAGTTAGGATACATTGGAGAGGGAGAAAGAAAGAGGGGAAGATGAAGTTTCTTCCTCTTTTTTCTTTTTTGGGTAAATGTGTGACTTCAATGGTAAACATTGTTGAAGTTGGTGAAGTTTGATTTATGTGACAAAGTTGAACCCGCTCATTTCTCTACTTTTGAAAATACTCAAAAAGCTTGCTTATGAATGTATAAAAACTATTAGCACTTATATACCATAAAGCTCTCTCTTTGAGCATACAATTAGGTTTAGAAAGTTCGTGTTTGGAGTGCAGAGTTATTTAGTTGGAAGTTACAAGACATTAGAGGGAGAGAGAAAGGAAGGAGAATGATGAAATTCCTCGATAAATATGCAAACTTCAATAGTGGAGTTGGTGAAATTTATGAAGTTCGTTAAACCAAACACTCCATAATTGCTTTACTTTTGAAACGACCCAAAAGGCTTATGTACCAATAATTGTTAGTACTTAAATACCATGAATCTCTCTCTTTCTTACTCGATGTGGGACTTTGTTTGTACTCCTAACACGTGCCAACTAATAGAGCTTTAAATTGATGATTGTTACACTAGAGAGCATGTTCAACTTGTGTTTTATTTGAAGAAGCTTAGTTTGAACATAACCTTGGAAGTGTGGAACATTTTGACAGACAAAAATAATGAAGGAATACCCAGAAGAGGCAACAGTGGTCTTCTTCTACAACTTAACTGTGGCCATCATAGCTGCATTTGTAGGTTTGATTTTAGAGAAAGATCCAAGTGCTTGGATTTTGAGGCAAAATACAGCATTAGCCTCTATTTTATGCTCTGTAAGAACCAACAAAAAGTTACTTAATAAATGTCACTGTCCTCTTATAGTCTATCATCTAACCAAAACTTTTTTTTCTTTCCTATATTTTACAGGGACTTTTTGGATCATGTTTAAACAATACAGTTCATACATGGGCACTCCGATTGAAAGGGCCGGTGTTCGTAGCAATGTTCAAGCCGTTGTCGATTGTTGTTGCTATAGTAATGGGTGTTCTGTTTCTTGGTGATTCTCTATATCTTGGAAGGTATGACCACAAAGAAAAAGAGAGAGTTTCATGAGTTGGGTTTTTTTATTTTGACCTATATGGCTGATATATAACTCTATAACCACAAGTTTCTTGCATCAGTTAAACTAACAGGAAGAAATTTTCCTTACAGTCTTATTGGAGCATCCATAATATCCATAGGGTTTTATACTGTAATGTGGGGAAAAGCAAAAGAAGAAATGGGTGTTGGAGAAGAAAAACAAGAAGGCCATAGCCATAATAATCTTGATGGTAACAAGGAATCAAATGAAGATCAGAGAGTACCTCTTCTTGGAAGCTACAACAGATACAGTGAAGATCATGTATGAAAAAAGTCAAGAAAATGATTGATGATTCAACTTTTATTTACCTTCAAGCTATGGCAATGGCTTTGGCCAACTGGTGGAAATTGTACAATTAGTTTGTGGTCATCAAAACATGTCATGGGGTAGTAAAGATTTTTAACACTTTTCACCATACCAATGGAAAGGTTAATTTATATGTACTCTACAATTTCAAATGATTTTGAAATTGTGGCTATGAAAAAGGTACAAGTTTTATTTTGCCTTCAAGCTATGGCTATGGCCAAGCATGGGAGGATGAATTGTAAAATTTGTCTGTGGTTAGAGAATGTGTTAGGGTTGTAAACATTTTTTACACTTTCGACCATGTGACAATGAAACATTTTAGTTTAAGATGTACACACTACAATTCAAATGATTTTGATTCTAC

mRNA sequence

GTCGGAATTCCAACCTCTAATTAAAAATATCAATAATTAGTGGATTTGCACAATCATCTTTTATAAATAGATTTATACATAAAACCCATTGAAGTTCAAAAGAGAAGACTCTGTTTCTTCTCTAAACAAACAGAACAAGTTTTCATGGCGTTAACTGGAAGAAGCTTAATGGAAGGAGTTCTTCCATTTCCGGCGATGATGGCGATGGAGTGTATAAACGTCGGTCTCAACACACTTTTTAAAGCCGCCACTGCCGCCGGGATGAGCCACCACGTCTTCGTCGTTTACTCTTACTCTTTCGCCGCCCTTCTGCTCCTCCCTTCTCCTTTCATCTCTCGAACATCCACTCGCCTTCCACCTCTCAATTTCTCCATAACTTCAAAGATTGCTCTGCTTGGACTAATCGGTTCTTGTTCGCAGATCATGGGATACACAGGAATCAATTATAGTTCTCCAACACTTGCTTCCGCCATCAGCAACCTTGTTCCTGCTTTCACTTTCATTCTCGCCATCATTTTCAGGATGGAGAAAGTGGTATTGAAAAGCAATAGCAGTCAAGCAAAAGTGATGGGCACAATAATATCAATATCAGGGGCATTTGTGGTAACATTTTACAAGGGTCCAAGAATCATATTCTCATCTTCACCAACCATTTCTCTTTCACTTCATCACAATTCACAACAACTATCCTCTTCTGATTCTAATTGGGTTATTGGTAGCCTTCTCTTGACTGCTGAATATATCCTTGTTCCACTTTGGTACATTGTTCAGACAAAAATAATGAAGGAATACCCAGAAGAGGCAACAGTGGTCTTCTTCTACAACTTAACTGTGGCCATCATAGCTGCATTTGTAGGTTTGATTTTAGAGAAAGATCCAAGTGCTTGGATTTTGAGGCAAAATACAGCATTAGCCTCTATTTTATGCTCTGGACTTTTTGGATCATGTTTAAACAATACAGTTCATACATGGGCACTCCGATTGAAAGGGCCGGTGTTCGTAGCAATGTTCAAGCCGTTGTCGATTGTTGTTGCTATAGTAATGGGTGTTCTGTTTCTTGGTGATTCTCTATATCTTGGAAGTCTTATTGGAGCATCCATAATATCCATAGGGTTTTATACTGTAATGTGGGGAAAAGCAAAAGAAGAAATGGGTGTTGGAGAAGAAAAACAAGAAGGCCATAGCCATAATAATCTTGATGGTAACAAGGAATCAAATGAAGATCAGAGAGTACCTCTTCTTGGAAGCTACAACAGATACAGTGAAGATCATGTATGAAAAAAGTCAAGAAAATGATTGATGATTCAACTTTTATTTACCTTCAAGCTATGGCAATGGCTTTGGCCAACTGGTGGAAATTGTACAATTAGTTTGTGGTCATCAAAACATGTCATGGGGTAGTAAAGATTTTTAACACTTTTCACCATACCAATGGAAAGGTTAATTTATATGTACTCTACAATTTCAAATGATTTTGAAATTGTGGCTATGAAAAAGGTACAAGTTTTATTTTGCCTTCAAGCTATGGCTATGGCCAAGCATGGGAGGATGAATTGTAAAATTTGTCTGTGGTTAGAGAATGTGTTAGGGTTGTAAACATTTTTTACACTTTCGACCATGTGACAATGAAACATTTTAGTTTAAGATGTACACACTACAATTCAAATGATTTTGATTCTAC

Coding sequence (CDS)

ATGGCGTTAACTGGAAGAAGCTTAATGGAAGGAGTTCTTCCATTTCCGGCGATGATGGCGATGGAGTGTATAAACGTCGGTCTCAACACACTTTTTAAAGCCGCCACTGCCGCCGGGATGAGCCACCACGTCTTCGTCGTTTACTCTTACTCTTTCGCCGCCCTTCTGCTCCTCCCTTCTCCTTTCATCTCTCGAACATCCACTCGCCTTCCACCTCTCAATTTCTCCATAACTTCAAAGATTGCTCTGCTTGGACTAATCGGTTCTTGTTCGCAGATCATGGGATACACAGGAATCAATTATAGTTCTCCAACACTTGCTTCCGCCATCAGCAACCTTGTTCCTGCTTTCACTTTCATTCTCGCCATCATTTTCAGGATGGAGAAAGTGGTATTGAAAAGCAATAGCAGTCAAGCAAAAGTGATGGGCACAATAATATCAATATCAGGGGCATTTGTGGTAACATTTTACAAGGGTCCAAGAATCATATTCTCATCTTCACCAACCATTTCTCTTTCACTTCATCACAATTCACAACAACTATCCTCTTCTGATTCTAATTGGGTTATTGGTAGCCTTCTCTTGACTGCTGAATATATCCTTGTTCCACTTTGGTACATTGTTCAGACAAAAATAATGAAGGAATACCCAGAAGAGGCAACAGTGGTCTTCTTCTACAACTTAACTGTGGCCATCATAGCTGCATTTGTAGGTTTGATTTTAGAGAAAGATCCAAGTGCTTGGATTTTGAGGCAAAATACAGCATTAGCCTCTATTTTATGCTCTGGACTTTTTGGATCATGTTTAAACAATACAGTTCATACATGGGCACTCCGATTGAAAGGGCCGGTGTTCGTAGCAATGTTCAAGCCGTTGTCGATTGTTGTTGCTATAGTAATGGGTGTTCTGTTTCTTGGTGATTCTCTATATCTTGGAAGTCTTATTGGAGCATCCATAATATCCATAGGGTTTTATACTGTAATGTGGGGAAAAGCAAAAGAAGAAATGGGTGTTGGAGAAGAAAAACAAGAAGGCCATAGCCATAATAATCTTGATGGTAACAAGGAATCAAATGAAGATCAGAGAGTACCTCTTCTTGGAAGCTACAACAGATACAGTGAAGATCATGTATGA

Protein sequence

MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQLSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILEKDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNEDQRVPLLGSYNRYSEDHV*
Homology
BLAST of CSPI05G28280 vs. ExPASy Swiss-Prot
Match: Q94JU2 (WAT1-related protein At3g28050 OS=Arabidopsis thaliana OX=3702 GN=At3g28050 PE=2 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 4.5e-115
Identity = 222/367 (60.49%), Postives = 271/367 (73.84%), Query Frame = 0

Query: 12  VLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRTSTRLP 71
           VLP  A++ MEC NVGLNTLFKAAT  GMS HVF+VYSY  AALLLLPS F S  S  LP
Sbjct: 10  VLPVTALVIMECANVGLNTLFKAATLKGMSFHVFIVYSYGLAALLLLPSLFCSFRSRTLP 69

Query: 72  PLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFRMEKVV 131
           P+NFSI  KI LLG+IG CS IMGYTGINYSSPTLASAISNL PAFTF+LA++FRME V 
Sbjct: 70  PMNFSILYKIVLLGIIGCCSNIMGYTGINYSSPTLASAISNLTPAFTFLLAVVFRMESVS 129

Query: 132 LKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQLSSSDSNWVIG 191
            K  SS AK++GT++SI GAF+VT Y GP +I  S P++SL       +  S++ NW++G
Sbjct: 130 FKRTSSVAKMLGTVVSIGGAFIVTLYNGPVVIAKSPPSVSL-------RSQSTNPNWILG 189

Query: 192 SLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILE-KDPSAWIL 251
           +  L  EY  VPLWYIVQT+IM+EYP E TVV FY++ V+   A V L  E  D  AW +
Sbjct: 190 AGFLAVEYFCVPLWYIVQTQIMREYPAEFTVVCFYSIGVSFWTALVTLFTEGNDLGAWKI 249

Query: 252 RQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLGDSLY 311
           + N AL SI+CSGLFGSC+NNT+HTWALR+KGP+FVAMFKPLSI +A+ MGV+FL DSLY
Sbjct: 250 KPNIALVSIVCSGLFGSCINNTIHTWALRIKGPLFVAMFKPLSIAIAVAMGVIFLRDSLY 309

Query: 312 LGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNEDQRVPLLGSYN 371
           +GSLIGA++I+IGFYTVMWGKAKE   V ++ +  H   N       +  Q+ PLL SY 
Sbjct: 310 IGSLIGATVITIGFYTVMWGKAKEVALVEDDNKANHEEANEADLDSPSGSQKAPLLESYK 367

Query: 372 RYSEDHV 378
             +++HV
Sbjct: 370 --NDEHV 367

BLAST of CSPI05G28280 vs. ExPASy Swiss-Prot
Match: F4KHA8 (WAT1-related protein At5g40230 OS=Arabidopsis thaliana OX=3702 GN=At5g40230 PE=3 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.2e-94
Identity = 185/329 (56.23%), Postives = 246/329 (74.77%), Query Frame = 0

Query: 12  VLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRTSTRLP 71
           V+PF AM+A+EC+ VG NTLFKAAT  G+S +VFV Y+Y  A L+LLP   I   S RLP
Sbjct: 19  VVPFTAMVAVECVTVGSNTLFKAATLRGLSFYVFVFYTYVVATLVLLPLSLIFGRSKRLP 78

Query: 72  PLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFRMEKVV 131
                +   I LL L+G  S I+G  GI YSSPTLASAISNL PAFTF LA+IFRME++V
Sbjct: 79  SAKTPVFFNIFLLALVGFMSLIVGCKGIEYSSPTLASAISNLTPAFTFTLAVIFRMEQIV 138

Query: 132 LKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSS-----SPTISLSLHHNSQQLSSSDS 191
           L+S+++QAK++GTI+SISGA VV  YKGP+++  +     SPTISL  H     L+S DS
Sbjct: 139 LRSSATQAKIIGTIVSISGALVVILYKGPKVLTDASLTPPSPTISLYQH-----LTSFDS 198

Query: 192 NWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILEKDPS 251
           +W+IG LLL  +Y+LV +WYI+QT++M+ YPEE TVVF YNL   +I+A V L  EKD +
Sbjct: 199 SWIIGGLLLATQYLLVSVWYILQTRVMELYPEEITVVFLYNLCATLISAPVCLFAEKDLN 258

Query: 252 AWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLG 311
           ++IL+   +LAS++ SG   S   + +HTW L LKGPV++++FKPLSIV+A+ MGV+FLG
Sbjct: 259 SFILKPGVSLASVMYSGGLVSSFGSVIHTWGLHLKGPVYISLFKPLSIVIAVAMGVMFLG 318

Query: 312 DSLYLGSLIGASIISIGFYTVMWGKAKEE 336
           D+LYLGS+IG+ I+S+GFYTV+WGKA+E+
Sbjct: 319 DALYLGSVIGSLILSLGFYTVIWGKARED 342

BLAST of CSPI05G28280 vs. ExPASy Swiss-Prot
Match: Q9FL08 (WAT1-related protein At5g40240 OS=Arabidopsis thaliana OX=3702 GN=At5g40240 PE=2 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 3.7e-93
Identity = 180/324 (55.56%), Postives = 241/324 (74.38%), Query Frame = 0

Query: 12  VLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRTSTRLP 71
           V+PF AM A+EC  VG NTLFKAAT  G+S +VFV YSY  + LLLLP   I   S RLP
Sbjct: 18  VVPFAAMFAVECATVGSNTLFKAATLRGLSFYVFVFYSYIVSTLLLLPLSVIFGRSRRLP 77

Query: 72  PLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFRMEKVV 131
                +  KI LLGL+G  SQI G  GI YSSPTLASAISNL PAFTF LA+IFRME+V 
Sbjct: 78  AAKSPLFFKIFLLGLVGFMSQIAGCKGIAYSSPTLASAISNLTPAFTFTLAVIFRMEQVR 137

Query: 132 LKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQLSSSDSNWVIG 191
           L+S+++QAK++G I+SISGA VV  YKGP+++ S+S T  L      QQL+S +S+W+IG
Sbjct: 138 LRSSATQAKIIGAILSISGALVVVLYKGPQVLASASFTTVLPTVTLHQQLTSIESSWIIG 197

Query: 192 SLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILEKDPSAWILR 251
            LLL ++Y L+ +WYI+QT++M+ YPEE TVVFFYNL   +I+  V L  E + ++W+L+
Sbjct: 198 GLLLASQYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNLTSWVLK 257

Query: 252 QNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLGDSLYL 311
            + +LA+I+ SG+F S  +   HTW L LKGPV++++F+PLSI +A+ MG +FLGD+L+L
Sbjct: 258 PDISLAAIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFLGDALHL 317

Query: 312 GSLIGASIISIGFYTVMWGKAKEE 336
           GS+IG+ I+ IGFYTV+WGKA+E+
Sbjct: 318 GSVIGSMILCIGFYTVIWGKARED 341

BLAST of CSPI05G28280 vs. ExPASy Swiss-Prot
Match: F4JK59 (WAT1-related protein At4g15540 OS=Arabidopsis thaliana OX=3702 GN=At4g15540 PE=2 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.1e-84
Identity = 165/324 (50.93%), Postives = 234/324 (72.22%), Query Frame = 0

Query: 12  VLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRTSTRLP 71
           V+PF AM+A+EC  VG + L+KAAT  G S +VFV Y+Y  A L+LL    I   S  LP
Sbjct: 15  VVPFTAMIAIECTTVGSSILYKAATLRGFSFYVFVFYAYVGATLVLLLLSLIFGRSRSLP 74

Query: 72  PLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFRMEKVV 131
               S+  KI LL L+G  S++ G  GI YSSPTL+SAISNL PAFTFILAI FRME+V+
Sbjct: 75  TAKSSLFFKIFLLALLGLTSRVAGCKGIEYSSPTLSSAISNLTPAFTFILAIFFRMEQVM 134

Query: 132 LKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQLSSSDSNWVIG 191
           L+S+++QAK++GTI+SISGA V+  YKGP+++ ++S              +S +S+W+IG
Sbjct: 135 LRSSATQAKIIGTIVSISGALVIVLYKGPKLLVAAS-------------FTSFESSWIIG 194

Query: 192 SLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILEKDPSAWILR 251
            LLL  +++L+ +W+I+QT IM+ YPEE  VVF YNL   +I+  V L++EKD ++W L+
Sbjct: 195 GLLLGLQFLLLSVWFILQTHIMEIYPEEIAVVFCYNLCATLISGTVCLLVEKDLNSWQLK 254

Query: 252 QNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLGDSLYL 311
              +LAS++ SGLF + L + +HTW L +KGPV++++FKPLSI +A+ M  +FLGD+L+L
Sbjct: 255 PGFSLASVIYSGLFDTSLGSVIHTWGLHVKGPVYISLFKPLSIAIAVAMAAIFLGDTLHL 314

Query: 312 GSLIGASIISIGFYTVMWGKAKEE 336
           GS+IG+ I+S GFYTV+WGKA+E+
Sbjct: 315 GSVIGSVILSFGFYTVIWGKARED 325

BLAST of CSPI05G28280 vs. ExPASy Swiss-Prot
Match: F4IYZ0 (WAT1-related protein At3g28080 OS=Arabidopsis thaliana OX=3702 GN=At3g28080 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.1e-83
Identity = 174/320 (54.37%), Postives = 230/320 (71.88%), Query Frame = 0

Query: 17  AMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRTSTRLPPLNFS 76
           AM+A E   VGL+TLFK AT+ G++ + F+ YSY  A+LLLLPS F +  S  LPPL+ S
Sbjct: 17  AMLAGETSIVGLSTLFKVATSKGLNIYPFLSYSYLLASLLLLPSLFFTNRSRSLPPLSAS 76

Query: 77  ITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFRMEKVVLKSNS 136
           I SKI LLG +GS   I G  GI YS+PTLASAI N+VPA TFILA+IFRMEKV  K  S
Sbjct: 77  ILSKIGLLGFLGSMYVITGGIGIEYSNPTLASAIGNIVPALTFILAVIFRMEKVSFKERS 136

Query: 137 SQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQLSSSDSNWVIGSLLLT 196
           S AKVMGTI+S+ GAFVV FY GPR+  +SSP   L+    S  LSSS S+W+IG  +LT
Sbjct: 137 SVAKVMGTILSLIGAFVVIFYHGPRVFVASSPPY-LNFRQLSPPLSSSKSDWLIGGAILT 196

Query: 197 AEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILEK-DPSAWILRQNTA 256
            + I V + +I+QT IM+EYPE  TV   Y L ++I+ + +GL++EK +PS WI+  +  
Sbjct: 197 IQGIFVSVSFILQTHIMREYPEAFTVSILYILCISIVTSMIGLVVEKNNPSIWIIHFDIT 256

Query: 257 LASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLGDSLYLGSLI 316
           L +I+ +G+  S +   +H+WA+R K P+++A+FKPLSI++A+VMG +FL DSLYLG LI
Sbjct: 257 LFTIVTTGIITS-VYYVIHSWAIRHKRPLYLAIFKPLSILIAVVMGTIFLNDSLYLGCLI 316

Query: 317 GASIISIGFYTVMWGKAKEE 336
           G  +I++GFY VMWGKA EE
Sbjct: 317 GGILITLGFYVVMWGKANEE 334

BLAST of CSPI05G28280 vs. ExPASy TrEMBL
Match: A0A1S3C8J8 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103498056 PE=3 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 4.8e-200
Identity = 370/377 (98.14%), Postives = 372/377 (98.67%), Query Frame = 0

Query: 1   MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPS 60
           MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATA GMSHHVFVVYSYSFAALLLLPS
Sbjct: 1   MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATATGMSHHVFVVYSYSFAALLLLPS 60

Query: 61  PFISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFI 120
           PFISR STRLPPLNFS+ SKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFI
Sbjct: 61  PFISRRSTRLPPLNFSVISKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFI 120

Query: 121 LAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ 180
           LAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ
Sbjct: 121 LAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ 180

Query: 181 LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLI 240
           LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTV IIAAFVG I
Sbjct: 181 LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVGIIAAFVGFI 240

Query: 241 LEKDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM 300
           LEKDPSAWILRQNTALAS+LCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM
Sbjct: 241 LEKDPSAWILRQNTALASVLCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM 300

Query: 301 GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED 360
           GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED
Sbjct: 301 GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED 360

Query: 361 QRVPLLGSYNRYSEDHV 378
           QRVPLLGSYNRYSEDHV
Sbjct: 361 QRVPLLGSYNRYSEDHV 377

BLAST of CSPI05G28280 vs. ExPASy TrEMBL
Match: A0A5A7T283 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00480 PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 6.5e-189
Identity = 355/377 (94.16%), Postives = 357/377 (94.69%), Query Frame = 0

Query: 1   MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPS 60
           MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATA GMSHHVFVVYSYSFAALLLLPS
Sbjct: 1   MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATATGMSHHVFVVYSYSFAALLLLPS 60

Query: 61  PFISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFI 120
           PFISR STRLPPLNFS+ SKIALLGLIGSCSQIMGYTGINYSSPTLASAISNL       
Sbjct: 61  PFISRRSTRLPPLNFSVISKIALLGLIGSCSQIMGYTGINYSSPTLASAISNL------- 120

Query: 121 LAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ 180
                   KVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ
Sbjct: 121 --------KVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ 180

Query: 181 LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLI 240
           LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTV IIAAFVG I
Sbjct: 181 LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVGIIAAFVGFI 240

Query: 241 LEKDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM 300
           LEKDPSAWILRQNTALAS+LCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM
Sbjct: 241 LEKDPSAWILRQNTALASVLCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM 300

Query: 301 GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED 360
           GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED
Sbjct: 301 GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED 360

Query: 361 QRVPLLGSYNRYSEDHV 378
           QRVPLLGSYNRYSEDHV
Sbjct: 361 QRVPLLGSYNRYSEDHV 362

BLAST of CSPI05G28280 vs. ExPASy TrEMBL
Match: A0A0A0KX55 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_5G630930 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 6.5e-181
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 40  MSHHVFVVYSYSFAALLLLPSPFISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGI 99
           MSHHVFVVYSYSFAALLLLPSPFISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGI
Sbjct: 1   MSHHVFVVYSYSFAALLLLPSPFISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGI 60

Query: 100 NYSSPTLASAISNLVPAFTFILAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKG 159
           NYSSPTLASAISNLVPAFTFILAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKG
Sbjct: 61  NYSSPTLASAISNLVPAFTFILAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKG 120

Query: 160 PRIIFSSSPTISLSLHHNSQQLSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEE 219
           PRIIFSSSPTISLSLHHNSQQLSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEE
Sbjct: 121 PRIIFSSSPTISLSLHHNSQQLSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEE 180

Query: 220 ATVVFFYNLTVAIIAAFVGLILEKDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALR 279
           ATVVFFYNLTVAIIAAFVGLILEKDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALR
Sbjct: 181 ATVVFFYNLTVAIIAAFVGLILEKDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALR 240

Query: 280 LKGPVFVAMFKPLSIVVAIVMGVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVG 339
           LKGPVFVAMFKPLSIVVAIVMGVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVG
Sbjct: 241 LKGPVFVAMFKPLSIVVAIVMGVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVG 300

Query: 340 EEKQEGHSHNNLDGNKESNEDQRVPLLGSYNRYSEDHV 378
           EEKQEGHSHNNLDGNKESNEDQRVPLLGSYNRYSEDHV
Sbjct: 301 EEKQEGHSHNNLDGNKESNEDQRVPLLGSYNRYSEDHV 338

BLAST of CSPI05G28280 vs. ExPASy TrEMBL
Match: A0A6J1GM35 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111455487 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.8e-170
Identity = 326/377 (86.47%), Postives = 346/377 (91.78%), Query Frame = 0

Query: 3   LTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPF 62
           + G   MEGVLPFPAM+ MECINVGLNTLFKAATA+GMSHHVF+VYSYS AA  LLPSPF
Sbjct: 1   MAGSYFMEGVLPFPAMVTMECINVGLNTLFKAATASGMSHHVFIVYSYSLAAFFLLPSPF 60

Query: 63  ISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILA 122
           ISR STRLPPLNFSI SKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILA
Sbjct: 61  ISRRSTRLPPLNFSILSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILA 120

Query: 123 IIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQLS 182
           IIFRMEKVV+KS+SSQAKVMGTIISISGAFVVT YKGPR+IFSS PT SLSLHH    L+
Sbjct: 121 IIFRMEKVVMKSSSSQAKVMGTIISISGAFVVTLYKGPRVIFSSPPT-SLSLHH---PLT 180

Query: 183 SSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILE 242
           SSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATV+FFYN+TVA IAA VG+ILE
Sbjct: 181 SSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVIFFYNITVAFIAAVVGVILE 240

Query: 243 KDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGV 302
            DPSAWILRQNTALAS+LCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGV
Sbjct: 241 TDPSAWILRQNTALASVLCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGV 300

Query: 303 LFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNEDQR 362
           LFLGDSLYLGSLIGASIISIGFYTVMWGK +EE+G  EE++E HSHN  D +KE +EDQ+
Sbjct: 301 LFLGDSLYLGSLIGASIISIGFYTVMWGKTREEVG-DEEEKERHSHNVSD-DKEPHEDQK 360

Query: 363 VPLLGSYN--RYSEDHV 378
           VPLLG+YN  RYS DHV
Sbjct: 361 VPLLGNYNSQRYSSDHV 371

BLAST of CSPI05G28280 vs. ExPASy TrEMBL
Match: A0A6J1FI76 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111445562 PE=3 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 4.3e-169
Identity = 323/379 (85.22%), Postives = 350/379 (92.35%), Query Frame = 0

Query: 1   MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPS 60
           MA +G  LME  LPFPAM++MECINVGLNTLFKAATA+GMSHHVFVVYSYS AALLLLPS
Sbjct: 1   MAGSGTYLMEAALPFPAMISMECINVGLNTLFKAATASGMSHHVFVVYSYSLAALLLLPS 60

Query: 61  PFISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFI 120
           PFISR S RLPPL+FS+ SKIALLGLIGS SQIMGYTGINYSSPTLASAISNLVPAFTFI
Sbjct: 61  PFISRRSRRLPPLSFSVLSKIALLGLIGSSSQIMGYTGINYSSPTLASAISNLVPAFTFI 120

Query: 121 LAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ 180
           LA+IFRMEKVVL+ +SSQAKVMGTIISISGAFVVT YKGPRI+ SS+PT +LSLHH    
Sbjct: 121 LAVIFRMEKVVLRRHSSQAKVMGTIISISGAFVVTLYKGPRIMLSSAPT-ALSLHH---P 180

Query: 181 LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLI 240
           L SS SNWVIGSLLLTAEYILVPLWYIVQT+IMKEYP+EATV+FFYNLTVA IAA VG++
Sbjct: 181 LPSSHSNWVIGSLLLTAEYILVPLWYIVQTRIMKEYPDEATVIFFYNLTVAFIAAIVGVV 240

Query: 241 LEKDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM 300
           LE DPSAWIL+Q+TALAS+LCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM
Sbjct: 241 LETDPSAWILKQHTALASVLCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM 300

Query: 301 GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED 360
           GVLFLGDSLYLGS++GASIISIGFYTVMWGKAKEE GVGEEK+EGH+H NL GNKES+ED
Sbjct: 301 GVLFLGDSLYLGSVVGASIISIGFYTVMWGKAKEERGVGEEKEEGHTH-NLSGNKESHED 360

Query: 361 QRVPLLGSYN--RYSEDHV 378
           QRVPLLGSYN  R+S D+V
Sbjct: 361 QRVPLLGSYNTHRHSADNV 374

BLAST of CSPI05G28280 vs. NCBI nr
Match: XP_004142351.1 (WAT1-related protein At3g28050 isoform X1 [Cucumis sativus] >KAE8648895.1 hypothetical protein Csa_008666 [Cucumis sativus])

HSP 1 Score: 716.8 bits (1849), Expect = 9.5e-203
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPS 60
           MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPS
Sbjct: 1   MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPS 60

Query: 61  PFISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFI 120
           PFISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFI
Sbjct: 61  PFISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFI 120

Query: 121 LAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ 180
           LAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ
Sbjct: 121 LAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ 180

Query: 181 LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLI 240
           LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLI
Sbjct: 181 LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLI 240

Query: 241 LEKDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM 300
           LEKDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM
Sbjct: 241 LEKDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM 300

Query: 301 GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED 360
           GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED
Sbjct: 301 GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED 360

Query: 361 QRVPLLGSYNRYSEDHV 378
           QRVPLLGSYNRYSEDHV
Sbjct: 361 QRVPLLGSYNRYSEDHV 377

BLAST of CSPI05G28280 vs. NCBI nr
Match: XP_008458740.1 (PREDICTED: WAT1-related protein At3g28050-like [Cucumis melo])

HSP 1 Score: 706.8 bits (1823), Expect = 9.8e-200
Identity = 370/377 (98.14%), Postives = 372/377 (98.67%), Query Frame = 0

Query: 1   MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPS 60
           MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATA GMSHHVFVVYSYSFAALLLLPS
Sbjct: 1   MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATATGMSHHVFVVYSYSFAALLLLPS 60

Query: 61  PFISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFI 120
           PFISR STRLPPLNFS+ SKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFI
Sbjct: 61  PFISRRSTRLPPLNFSVISKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFI 120

Query: 121 LAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ 180
           LAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ
Sbjct: 121 LAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ 180

Query: 181 LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLI 240
           LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTV IIAAFVG I
Sbjct: 181 LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVGIIAAFVGFI 240

Query: 241 LEKDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM 300
           LEKDPSAWILRQNTALAS+LCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM
Sbjct: 241 LEKDPSAWILRQNTALASVLCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM 300

Query: 301 GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED 360
           GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED
Sbjct: 301 GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED 360

Query: 361 QRVPLLGSYNRYSEDHV 378
           QRVPLLGSYNRYSEDHV
Sbjct: 361 QRVPLLGSYNRYSEDHV 377

BLAST of CSPI05G28280 vs. NCBI nr
Match: KAA0037480.1 (WAT1-related protein [Cucumis melo var. makuwa] >TYK01956.1 WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 669.8 bits (1727), Expect = 1.3e-188
Identity = 355/377 (94.16%), Postives = 357/377 (94.69%), Query Frame = 0

Query: 1   MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPS 60
           MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATA GMSHHVFVVYSYSFAALLLLPS
Sbjct: 1   MALTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATATGMSHHVFVVYSYSFAALLLLPS 60

Query: 61  PFISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFI 120
           PFISR STRLPPLNFS+ SKIALLGLIGSCSQIMGYTGINYSSPTLASAISNL       
Sbjct: 61  PFISRRSTRLPPLNFSVISKIALLGLIGSCSQIMGYTGINYSSPTLASAISNL------- 120

Query: 121 LAIIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ 180
                   KVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ
Sbjct: 121 --------KVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQ 180

Query: 181 LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLI 240
           LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTV IIAAFVG I
Sbjct: 181 LSSSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVGIIAAFVGFI 240

Query: 241 LEKDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM 300
           LEKDPSAWILRQNTALAS+LCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM
Sbjct: 241 LEKDPSAWILRQNTALASVLCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVM 300

Query: 301 GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED 360
           GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED
Sbjct: 301 GVLFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNED 360

Query: 361 QRVPLLGSYNRYSEDHV 378
           QRVPLLGSYNRYSEDHV
Sbjct: 361 QRVPLLGSYNRYSEDHV 362

BLAST of CSPI05G28280 vs. NCBI nr
Match: XP_038890135.1 (WAT1-related protein At3g28050-like [Benincasa hispida])

HSP 1 Score: 635.6 bits (1638), Expect = 2.8e-178
Identity = 343/373 (91.96%), Postives = 355/373 (95.17%), Query Frame = 0

Query: 7   SLMEGVLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRT 66
           SLMEG+LPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISR 
Sbjct: 4   SLMEGILPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRR 63

Query: 67  STRLPPLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFR 126
           STRLPPLN+S+ SKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFR
Sbjct: 64  STRLPPLNYSVISKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFR 123

Query: 127 MEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQLSSSDS 186
           MEKVVLKSNSSQAKV+GTIISISGAFVVTFYKGPRII SS+P ISLSLHH+  QLSS DS
Sbjct: 124 MEKVVLKSNSSQAKVVGTIISISGAFVVTFYKGPRIILSSAP-ISLSLHHH-PQLSSLDS 183

Query: 187 NWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILEKDPS 246
           NWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAA VG ILE DPS
Sbjct: 184 NWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAVVGAILETDPS 243

Query: 247 AWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLG 306
           AWILRQNTALAS+LCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLG
Sbjct: 244 AWILRQNTALASVLCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLG 303

Query: 307 DSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNEDQRVPLL 366
           DSL+LGSLIGASIISIGFYTVMWGKAKEEMGV EEK+EGHSHN    NKES+EDQRVPLL
Sbjct: 304 DSLHLGSLIGASIISIGFYTVMWGKAKEEMGVDEEKEEGHSHNL---NKESHEDQRVPLL 363

Query: 367 GSYN--RYSEDHV 378
           G+YN  RY+ DHV
Sbjct: 364 GTYNTQRYNADHV 371

BLAST of CSPI05G28280 vs. NCBI nr
Match: XP_023525248.1 (WAT1-related protein At3g28050-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 606.3 bits (1562), Expect = 1.8e-169
Identity = 326/377 (86.47%), Postives = 346/377 (91.78%), Query Frame = 0

Query: 3   LTGRSLMEGVLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPF 62
           + G   MEGVLPFPAM+ MECINVGLNTLFKAATA+GMSHHVF+VYSYS AA  LLPSPF
Sbjct: 1   MAGSYFMEGVLPFPAMVTMECINVGLNTLFKAATASGMSHHVFIVYSYSLAAFFLLPSPF 60

Query: 63  ISRTSTRLPPLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILA 122
           ISR STRLPPLNFSI SKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILA
Sbjct: 61  ISRRSTRLPPLNFSILSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILA 120

Query: 123 IIFRMEKVVLKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQLS 182
           IIFRMEKVV+KS+SSQAKVMGTIISISGAFVVT YKGPR+IFSS PT SLSLHH    L+
Sbjct: 121 IIFRMEKVVMKSSSSQAKVMGTIISISGAFVVTLYKGPRVIFSSPPT-SLSLHH---PLT 180

Query: 183 SSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILE 242
           SSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATV+FFYN+TVA IAA VG+ILE
Sbjct: 181 SSDSNWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVIFFYNITVAFIAAVVGVILE 240

Query: 243 KDPSAWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGV 302
            DPSAWILRQNTALAS+LCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGV
Sbjct: 241 TDPSAWILRQNTALASVLCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGV 300

Query: 303 LFLGDSLYLGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNEDQR 362
           LFLGDSLYLGSLIGASIISIGFYTVMWGK +EE+G  EE++E HSHN  D +KE +EDQ+
Sbjct: 301 LFLGDSLYLGSLIGASIISIGFYTVMWGKTREEVG-DEEEKERHSHNVSD-DKEPHEDQK 360

Query: 363 VPLLGSYN--RYSEDHV 378
           VPLLG+YN  RYS DHV
Sbjct: 361 VPLLGNYNTQRYSSDHV 371

BLAST of CSPI05G28280 vs. TAIR 10
Match: AT3G28050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 416.0 bits (1068), Expect = 3.2e-116
Identity = 222/367 (60.49%), Postives = 271/367 (73.84%), Query Frame = 0

Query: 12  VLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRTSTRLP 71
           VLP  A++ MEC NVGLNTLFKAAT  GMS HVF+VYSY  AALLLLPS F S  S  LP
Sbjct: 10  VLPVTALVIMECANVGLNTLFKAATLKGMSFHVFIVYSYGLAALLLLPSLFCSFRSRTLP 69

Query: 72  PLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFRMEKVV 131
           P+NFSI  KI LLG+IG CS IMGYTGINYSSPTLASAISNL PAFTF+LA++FRME V 
Sbjct: 70  PMNFSILYKIVLLGIIGCCSNIMGYTGINYSSPTLASAISNLTPAFTFLLAVVFRMESVS 129

Query: 132 LKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQLSSSDSNWVIG 191
            K  SS AK++GT++SI GAF+VT Y GP +I  S P++SL       +  S++ NW++G
Sbjct: 130 FKRTSSVAKMLGTVVSIGGAFIVTLYNGPVVIAKSPPSVSL-------RSQSTNPNWILG 189

Query: 192 SLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILE-KDPSAWIL 251
           +  L  EY  VPLWYIVQT+IM+EYP E TVV FY++ V+   A V L  E  D  AW +
Sbjct: 190 AGFLAVEYFCVPLWYIVQTQIMREYPAEFTVVCFYSIGVSFWTALVTLFTEGNDLGAWKI 249

Query: 252 RQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLGDSLY 311
           + N AL SI+CSGLFGSC+NNT+HTWALR+KGP+FVAMFKPLSI +A+ MGV+FL DSLY
Sbjct: 250 KPNIALVSIVCSGLFGSCINNTIHTWALRIKGPLFVAMFKPLSIAIAVAMGVIFLRDSLY 309

Query: 312 LGSLIGASIISIGFYTVMWGKAKEEMGVGEEKQEGHSHNNLDGNKESNEDQRVPLLGSYN 371
           +GSLIGA++I+IGFYTVMWGKAKE   V ++ +  H   N       +  Q+ PLL SY 
Sbjct: 310 IGSLIGATVITIGFYTVMWGKAKEVALVEDDNKANHEEANEADLDSPSGSQKAPLLESYK 367

Query: 372 RYSEDHV 378
             +++HV
Sbjct: 370 --NDEHV 367

BLAST of CSPI05G28280 vs. TAIR 10
Match: AT5G40230.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 348.2 bits (892), Expect = 8.3e-96
Identity = 185/329 (56.23%), Postives = 246/329 (74.77%), Query Frame = 0

Query: 12  VLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRTSTRLP 71
           V+PF AM+A+EC+ VG NTLFKAAT  G+S +VFV Y+Y  A L+LLP   I   S RLP
Sbjct: 19  VVPFTAMVAVECVTVGSNTLFKAATLRGLSFYVFVFYTYVVATLVLLPLSLIFGRSKRLP 78

Query: 72  PLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFRMEKVV 131
                +   I LL L+G  S I+G  GI YSSPTLASAISNL PAFTF LA+IFRME++V
Sbjct: 79  SAKTPVFFNIFLLALVGFMSLIVGCKGIEYSSPTLASAISNLTPAFTFTLAVIFRMEQIV 138

Query: 132 LKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSS-----SPTISLSLHHNSQQLSSSDS 191
           L+S+++QAK++GTI+SISGA VV  YKGP+++  +     SPTISL  H     L+S DS
Sbjct: 139 LRSSATQAKIIGTIVSISGALVVILYKGPKVLTDASLTPPSPTISLYQH-----LTSFDS 198

Query: 192 NWVIGSLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILEKDPS 251
           +W+IG LLL  +Y+LV +WYI+QT++M+ YPEE TVVF YNL   +I+A V L  EKD +
Sbjct: 199 SWIIGGLLLATQYLLVSVWYILQTRVMELYPEEITVVFLYNLCATLISAPVCLFAEKDLN 258

Query: 252 AWILRQNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLG 311
           ++IL+   +LAS++ SG   S   + +HTW L LKGPV++++FKPLSIV+A+ MGV+FLG
Sbjct: 259 SFILKPGVSLASVMYSGGLVSSFGSVIHTWGLHLKGPVYISLFKPLSIVIAVAMGVMFLG 318

Query: 312 DSLYLGSLIGASIISIGFYTVMWGKAKEE 336
           D+LYLGS+IG+ I+S+GFYTV+WGKA+E+
Sbjct: 319 DALYLGSVIGSLILSLGFYTVIWGKARED 342

BLAST of CSPI05G28280 vs. TAIR 10
Match: AT5G40240.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 343.2 bits (879), Expect = 2.7e-94
Identity = 180/324 (55.56%), Postives = 241/324 (74.38%), Query Frame = 0

Query: 12  VLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRTSTRLP 71
           V+PF AM A+EC  VG NTLFKAAT  G+S +VFV YSY  + LLLLP   I   S RLP
Sbjct: 18  VVPFAAMFAVECATVGSNTLFKAATLRGLSFYVFVFYSYIVSTLLLLPLSVIFGRSRRLP 77

Query: 72  PLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFRMEKVV 131
                +  KI LLGL+G  SQI G  GI YSSPTLASAISNL PAFTF LA+IFRME+V 
Sbjct: 78  AAKSPLFFKIFLLGLVGFMSQIAGCKGIAYSSPTLASAISNLTPAFTFTLAVIFRMEQVR 137

Query: 132 LKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQLSSSDSNWVIG 191
           L+S+++QAK++G I+SISGA VV  YKGP+++ S+S T  L      QQL+S +S+W+IG
Sbjct: 138 LRSSATQAKIIGAILSISGALVVVLYKGPQVLASASFTTVLPTVTLHQQLTSIESSWIIG 197

Query: 192 SLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILEKDPSAWILR 251
            LLL ++Y L+ +WYI+QT++M+ YPEE TVVFFYNL   +I+  V L  E + ++W+L+
Sbjct: 198 GLLLASQYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNLTSWVLK 257

Query: 252 QNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLGDSLYL 311
            + +LA+I+ SG+F S  +   HTW L LKGPV++++F+PLSI +A+ MG +FLGD+L+L
Sbjct: 258 PDISLAAIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFLGDALHL 317

Query: 312 GSLIGASIISIGFYTVMWGKAKEE 336
           GS+IG+ I+ IGFYTV+WGKA+E+
Sbjct: 318 GSVIGSMILCIGFYTVIWGKARED 341

BLAST of CSPI05G28280 vs. TAIR 10
Match: AT5G40240.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 343.2 bits (879), Expect = 2.7e-94
Identity = 180/324 (55.56%), Postives = 241/324 (74.38%), Query Frame = 0

Query: 12  VLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRTSTRLP 71
           V+PF AM A+EC  VG NTLFKAAT  G+S +VFV YSY  + LLLLP   I   S RLP
Sbjct: 32  VVPFAAMFAVECATVGSNTLFKAATLRGLSFYVFVFYSYIVSTLLLLPLSVIFGRSRRLP 91

Query: 72  PLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFRMEKVV 131
                +  KI LLGL+G  SQI G  GI YSSPTLASAISNL PAFTF LA+IFRME+V 
Sbjct: 92  AAKSPLFFKIFLLGLVGFMSQIAGCKGIAYSSPTLASAISNLTPAFTFTLAVIFRMEQVR 151

Query: 132 LKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQLSSSDSNWVIG 191
           L+S+++QAK++G I+SISGA VV  YKGP+++ S+S T  L      QQL+S +S+W+IG
Sbjct: 152 LRSSATQAKIIGAILSISGALVVVLYKGPQVLASASFTTVLPTVTLHQQLTSIESSWIIG 211

Query: 192 SLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILEKDPSAWILR 251
            LLL ++Y L+ +WYI+QT++M+ YPEE TVVFFYNL   +I+  V L  E + ++W+L+
Sbjct: 212 GLLLASQYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNLTSWVLK 271

Query: 252 QNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLGDSLYL 311
            + +LA+I+ SG+F S  +   HTW L LKGPV++++F+PLSI +A+ MG +FLGD+L+L
Sbjct: 272 PDISLAAIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFLGDALHL 331

Query: 312 GSLIGASIISIGFYTVMWGKAKEE 336
           GS+IG+ I+ IGFYTV+WGKA+E+
Sbjct: 332 GSVIGSMILCIGFYTVIWGKARED 355

BLAST of CSPI05G28280 vs. TAIR 10
Match: AT4G15540.1 (EamA-like transporter family )

HSP 1 Score: 315.1 bits (806), Expect = 7.8e-86
Identity = 165/324 (50.93%), Postives = 234/324 (72.22%), Query Frame = 0

Query: 12  VLPFPAMMAMECINVGLNTLFKAATAAGMSHHVFVVYSYSFAALLLLPSPFISRTSTRLP 71
           V+PF AM+A+EC  VG + L+KAAT  G S +VFV Y+Y  A L+LL    I   S  LP
Sbjct: 15  VVPFTAMIAIECTTVGSSILYKAATLRGFSFYVFVFYAYVGATLVLLLLSLIFGRSRSLP 74

Query: 72  PLNFSITSKIALLGLIGSCSQIMGYTGINYSSPTLASAISNLVPAFTFILAIIFRMEKVV 131
               S+  KI LL L+G  S++ G  GI YSSPTL+SAISNL PAFTFILAI FRME+V+
Sbjct: 75  TAKSSLFFKIFLLALLGLTSRVAGCKGIEYSSPTLSSAISNLTPAFTFILAIFFRMEQVM 134

Query: 132 LKSNSSQAKVMGTIISISGAFVVTFYKGPRIIFSSSPTISLSLHHNSQQLSSSDSNWVIG 191
           L+S+++QAK++GTI+SISGA V+  YKGP+++ ++S              +S +S+W+IG
Sbjct: 135 LRSSATQAKIIGTIVSISGALVIVLYKGPKLLVAAS-------------FTSFESSWIIG 194

Query: 192 SLLLTAEYILVPLWYIVQTKIMKEYPEEATVVFFYNLTVAIIAAFVGLILEKDPSAWILR 251
            LLL  +++L+ +W+I+QT IM+ YPEE  VVF YNL   +I+  V L++EKD ++W L+
Sbjct: 195 GLLLGLQFLLLSVWFILQTHIMEIYPEEIAVVFCYNLCATLISGTVCLLVEKDLNSWQLK 254

Query: 252 QNTALASILCSGLFGSCLNNTVHTWALRLKGPVFVAMFKPLSIVVAIVMGVLFLGDSLYL 311
              +LAS++ SGLF + L + +HTW L +KGPV++++FKPLSI +A+ M  +FLGD+L+L
Sbjct: 255 PGFSLASVIYSGLFDTSLGSVIHTWGLHVKGPVYISLFKPLSIAIAVAMAAIFLGDTLHL 314

Query: 312 GSLIGASIISIGFYTVMWGKAKEE 336
           GS+IG+ I+S GFYTV+WGKA+E+
Sbjct: 315 GSVIGSVILSFGFYTVIWGKARED 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94JU24.5e-11560.49WAT1-related protein At3g28050 OS=Arabidopsis thaliana OX=3702 GN=At3g28050 PE=2... [more]
F4KHA81.2e-9456.23WAT1-related protein At5g40230 OS=Arabidopsis thaliana OX=3702 GN=At5g40230 PE=3... [more]
Q9FL083.7e-9355.56WAT1-related protein At5g40240 OS=Arabidopsis thaliana OX=3702 GN=At5g40240 PE=2... [more]
F4JK591.1e-8450.93WAT1-related protein At4g15540 OS=Arabidopsis thaliana OX=3702 GN=At4g15540 PE=2... [more]
F4IYZ02.1e-8354.38WAT1-related protein At3g28080 OS=Arabidopsis thaliana OX=3702 GN=At3g28080 PE=2... [more]
Match NameE-valueIdentityDescription
A0A1S3C8J84.8e-20098.14WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103498056 PE=3 SV=1[more]
A0A5A7T2836.5e-18994.16WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808... [more]
A0A0A0KX556.5e-181100.00WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_5G630930 PE=3 SV=1[more]
A0A6J1GM358.8e-17086.47WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111455487 PE=3 SV=1[more]
A0A6J1FI764.3e-16985.22WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111445562 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004142351.19.5e-203100.00WAT1-related protein At3g28050 isoform X1 [Cucumis sativus] >KAE8648895.1 hypoth... [more]
XP_008458740.19.8e-20098.14PREDICTED: WAT1-related protein At3g28050-like [Cucumis melo][more]
KAA0037480.11.3e-18894.16WAT1-related protein [Cucumis melo var. makuwa] >TYK01956.1 WAT1-related protein... [more]
XP_038890135.12.8e-17891.96WAT1-related protein At3g28050-like [Benincasa hispida][more]
XP_023525248.11.8e-16986.47WAT1-related protein At3g28050-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G28050.13.2e-11660.49nodulin MtN21 /EamA-like transporter family protein [more]
AT5G40230.18.3e-9656.23nodulin MtN21 /EamA-like transporter family protein [more]
AT5G40240.12.7e-9455.56nodulin MtN21 /EamA-like transporter family protein [more]
AT5G40240.22.7e-9455.56nodulin MtN21 /EamA-like transporter family protein [more]
AT4G15540.17.8e-8650.93EamA-like transporter family [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 26..155
e-value: 4.3E-8
score: 33.4
coord: 201..328
e-value: 6.6E-9
score: 36.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 338..363
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 338..359
NoneNo IPR availablePANTHERPTHR31218:SF64WAT1-RELATED PROTEINcoord: 11..349
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 51..158
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 228..332
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 11..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G28280.1CSPI05G28280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity