CSPI05G28130 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G28130
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRNA exonuclease 4
LocationChr5: 26659195 .. 26663148 (+)
RNA-Seq ExpressionCSPI05G28130
SyntenyCSPI05G28130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGACTTCTTTTCACTCACATCATGAACCAACTTGTGCCATTTGCAAGAAACATTGTCGATCTCTTGATTCTCTTAGAGAACATCTCATTGGTAATGTCACTCTTCTAATTCATAATTGGACTTCATTTTTTTAAAAAATTTGGAACATACCTATGTCAACCAAAATATTTACAAAATTTAATTAAATATCATAGTATTTCTACGATGGACCGTGATAGACGCACATAGTGGTCTATCATGTCTATTATCGCAAATAGATTGTGATATATTTTTATTATATTTGTGAATATTTTTAGAAATTTTGTCATTTAAAATAATTTTTCTAGAAATTAACCCAAAAGTCTAATAAAAATTACAATATATTTTGTATCTAGTACTATGTTAAAAATTAATTTCTAAGCAGATGAGTTATGATAAATTTATTGTTAGAATATTATCTTTCCGATCTATGAACTTTGAGTTTCATCAATAAATTTTAAAGATAGTCCCTCTCTATATATATTTAGTTTAGTAAAAAACTAGTCTCTCTTAACTAGCTTTTTCGTTATTAATTTTGAGTATATTTTTTTCTCTACTAGTATAAGCTGTCTTCCTATTCAAAATATAAGTACTAAAATAAAATGAAACCTAAAGAATATAAATAATCAAATGATATTTTAACGTAGTGTAAAAATTTATAGATCAAGACTCTAACACATGTGAAATAAACATCAATATTTGAGAAAAAAATGATGTTATTAGGAGTTCGAACACCAAACTTCATTATCTAATATATTATTATGTTAAGTATCTAATATATTATTATGTTAAGAGCTTAAGCTGATGAGTTTACTATTCTTACGTTTTCGTTATTGTATTGTTATTAGGGCCACTGCCAAAACAAGAATGCAAAAACATCTTTGCTACAAGAGGATGCAAGTTTTGCTTGGCCATCTTTGACAGTTCTTACTCACAAAGACTCCACCAAGAAAGATGCCAATTCTCTCCTGTAAATTCTGTATGATTCCTAAAAAACCATTCTATTTAATCAAACAGAATGCATAAATATATATATATAATTCTTGTTGATTTTAAAATCTTGATGTTATTTTGCTTTTTGGGACATCAGGGACTTCTTGCTCGTTTCGCCAACTTGGGAATTCGTGACGGTTCAACCGTCATCGACAGTGGAAGAACACGAGGGTCAGGGGCCATCGCGCTAGCTTGCAAATATGTAGGTGGAGGAAGTGATGGGTCGATGGACATATGTGCTAAGGTTTGCCTTATTGATGAGGGTGAGAACGTCATCTTCTATAGCTATGTCAAGCCCGTAACTGCTGTTACAAATTATAGGTAAGTAAAATTAAATTCATGTATATAAATTTGAAGTTTAATATTATACTCCTTTTATTTTTATTTTTTTGAATTTGAGATTCATTTTTCGGTCTAAATTGTCGATTTTTTTAGAAAAGTTTAGGAACTGAATAAAAACTCATTTGACTATTTTCGAACTTAAAACATCAACAACTTTATAATTAATTTACTTTTTTTAGAAGGAAATTGAACCAATAAAGTAATTAATTTACTTTATAACTTTTAGAACCATGTGATCGAACTTAAGGTCAACATGTATTTATTATTAGAATTTAATCCCTAGTTAGAATTTAGTCATTATAGTTTAGAACGGCATAGATCAAGTTCAATCTAGTCTACTCCCGATTTATTGCATCTCATTCAAATTTTTTTGTCAGTTGATATATACTTTGAGTGTTGTTGGGGCGTCATAGATATAAAACAAAAAATTGGTGGAACTTAGAGTGAACTCAAGTTTTGTTTGGTTTAGTTTAACAGTACTTTTGATAAAACCCTAGACAATACCTAATTTTAATAGAAATAATTTACCAAACCATATTGACTTAAATTCAAACTTTGGTCAAACCAAATTTGCAATTTAACCTAATAATAATAACCCATAAAATTTTATTAAAATTTCCATTTATTTAGAAATGGAATTTTATCAAGCAAACACAAAATAAAAAAAATAAAATTATATATTGTTTTAACCTTTGATATGTTAAAATTAACATTAGGTATGAAACAACTGGAATTCGTCCAGAACACATTCGAGATGCAATACCAATGAAACAAGTTCAAAAGAAAATTCAAGAAATCCTCTACAATGGAGGTGAGAAGGCTAGGATTCTTGTAGGTGATTGCGTCGAAGACGATCTCAAACGCTTACAAATCGGTTACCCATCCTTCATGATAAGGTAAATTGCTCGTAATAAAATTTAAATTTGTCGTAACCTATCAACATAATTCATTTTAAAATTTCATATAAAAAATAACAAACATGATCTTCTAAGGATCAACTATATTTTGCATATATATATATATATATATATATATTACTTTTTTCTTGGTTGAATGCCAGATAAAATAGTAACTTATTTTTAAAAATAGGTTGAATTAGGAAATTCAAACTTCTAACATCTAAACAGATGTTAAAGTTTTTTTTTTCATTCCTAATATAAGCTATTAGTGTTGTTGGTGAAACCCTTTTAACATCTTATTGCATGTCGAAGTGAGCTCAACAGTGGCGGATCCAAAAATTTTATATAAGGGTATTGAACATTATATAATAAATACACTAAAACATATTTAAAAAATATATCGACGTTTAATCGATGCTTCAATAACATTCGCTACATTTGTAGCAACAAAGAAACATCTATAATTTCCACGTGATTTTTTGTAATGTAAATATGTAGATTTTTATGGAATGTTGTTGAGAGTAGAAGTTTTGACCAATGACTTTGTTGCATTTTTTTCTCTAAATATTTGTTAGATAACAAATGAGGGTTAGGAAATAAAGTAGGGTTTATAGAATGATTTTTTTTTTCTCTTTTTAAAAATTATATTCCTTTTACGAAATCATTTGAAATGAAAATTATTGATATTTGAAATAAAAATTATTTCAAAAAAATAATTGTCATAAGTAAGAATCAATTTTTTTAGGGCACAACCACTACCTCCCAACTAAAATTTAATTAAAATAAAGTATTATTTAATATATATTGTAATCTGACACTTCAAAATTAATATTAAAATACAATAATTGAGAATGTGAGAGGCACGTGTCCCTTTCGCCCCTTGAGCTCAAAACATTTAAGTTTGGTTTGATTTAGTTTTCTAGATTTTAATAATAAGTAGCGTTAATCTATAAGCTTAACTATTTAAAAAAAAAAAGTAAAAAAGTTATCAAACAACACCCTTAATTTGTTGATTTCATGCACTGACCCACAGGGACACAGCAAATTATCCACCATTGATGAAGTCAAGCAAGCTTTGCAATTCACTCAAGTATCTAGCTCAAGTGTATCTAGGGTAAGTTAACCTTTTCAGCACTCGTTTGTTTTTTTAAAGTCTTCCATAAAACTTATATTCTATTCATTTTTTTCATTTAAGGATTGAGATCCATGAAATAATATATAAATATATCATTTGATGGGTCAAATTGGTGAAAGTAAGAAGTAAGGCAGCTGATGTATTACAGCAGTACTAGAAAATTTTCCATATGTCGTTTTTCTCTATAGAAAATGATTAACTAAGAAGTGTTGTTAATTGTAGATTTGATCTTCAAAATGGTATACAAGATCCTTATGAAGAATGTATCGTTGCTATGAGATTATACAAGAGAATGAGATCACAAGTTCATAGGAAAGAGAACTATCCAATGGCTTCTGACCCACAAAACAAAAATAATTTTGCTATTTGGAGGGAAAATGAGCTTCAAAGGATGAGTCCAGAACAAATGTTGGAGATTTCTAGGTCTGATTATTATTGTTGGTGCATGGATTTGAGGATTTGAGAGCTTTCATACATTCTTCAACAATGGTGGAAATTTAATTTGGGTTGCCACAGCCCAATTAAGTTCAAGTTCTTCATTGTCAAGCAAATATAGATTTATT

mRNA sequence

ATGAGGACTTCTTTTCACTCACATCATGAACCAACTTGTGCCATTTGCAAGAAACATTGTCGATCTCTTGATTCTCTTAGAGAACATCTCATTGGGCCACTGCCAAAACAAGAATGCAAAAACATCTTTGCTACAAGAGGATGCAAGTTTTGCTTGGCCATCTTTGACAGTTCTTACTCACAAAGACTCCACCAAGAAAGATGCCAATTCTCTCCTGTAAATTCTGGACTTCTTGCTCGTTTCGCCAACTTGGGAATTCGTGACGGTTCAACCGTCATCGACAGTGGAAGAACACGAGGGTCAGGGGCCATCGCGCTAGCTTGCAAATATGTAGGTGGAGGAAGTGATGGGTCGATGGACATATGTGCTAAGGTTTGCCTTATTGATGAGGGTGAGAACGTCATCTTCTATAGCTATGTCAAGCCCGTAACTGCTGTTACAAATTATAGGTATGAAACAACTGGAATTCGTCCAGAACACATTCGAGATGCAATACCAATGAAACAAGTTCAAAAGAAAATTCAAGAAATCCTCTACAATGGAGGTGAGAAGGCTAGGATTCTTGTAGGTGATTGCGTCGAAGACGATCTCAAACGCTTACAAATCGGTTACCCATCCTTCATGATAAGGGACACAGCAAATTATCCACCATTGATGAAGTCAAGCAAGCTTTGCAATTCACTCAAGTATCTAGCTCAAGTGTATCTAGGATTTGATCTTCAAAATGGTATACAAGATCCTTATGAAGAATGTATCGTTGCTATGAGATTATACAAGAGAATGAGATCACAAGTTCATAGGAAAGAGAACTATCCAATGGCTTCTGACCCACAAAACAAAAATAATTTTGCTATTTGGAGGGAAAATGAGCTTCAAAGGATGAGTCCAGAACAAATGTTGGAGATTTCTAGGTCTGATTATTATTGTTGGTGCATGGATTTGAGGATTTGAGAGCTTTCATACATTCTTCAACAATGGTGGAAATTTAATTTGGGTTGCCACAGCCCAATTAAGTTCAAGTTCTTCATTGTCAAGCAAATATAGATTTATT

Coding sequence (CDS)

ATGAGGACTTCTTTTCACTCACATCATGAACCAACTTGTGCCATTTGCAAGAAACATTGTCGATCTCTTGATTCTCTTAGAGAACATCTCATTGGGCCACTGCCAAAACAAGAATGCAAAAACATCTTTGCTACAAGAGGATGCAAGTTTTGCTTGGCCATCTTTGACAGTTCTTACTCACAAAGACTCCACCAAGAAAGATGCCAATTCTCTCCTGTAAATTCTGGACTTCTTGCTCGTTTCGCCAACTTGGGAATTCGTGACGGTTCAACCGTCATCGACAGTGGAAGAACACGAGGGTCAGGGGCCATCGCGCTAGCTTGCAAATATGTAGGTGGAGGAAGTGATGGGTCGATGGACATATGTGCTAAGGTTTGCCTTATTGATGAGGGTGAGAACGTCATCTTCTATAGCTATGTCAAGCCCGTAACTGCTGTTACAAATTATAGGTATGAAACAACTGGAATTCGTCCAGAACACATTCGAGATGCAATACCAATGAAACAAGTTCAAAAGAAAATTCAAGAAATCCTCTACAATGGAGGTGAGAAGGCTAGGATTCTTGTAGGTGATTGCGTCGAAGACGATCTCAAACGCTTACAAATCGGTTACCCATCCTTCATGATAAGGGACACAGCAAATTATCCACCATTGATGAAGTCAAGCAAGCTTTGCAATTCACTCAAGTATCTAGCTCAAGTGTATCTAGGATTTGATCTTCAAAATGGTATACAAGATCCTTATGAAGAATGTATCGTTGCTATGAGATTATACAAGAGAATGAGATCACAAGTTCATAGGAAAGAGAACTATCCAATGGCTTCTGACCCACAAAACAAAAATAATTTTGCTATTTGGAGGGAAAATGAGCTTCAAAGGATGAGTCCAGAACAAATGTTGGAGATTTCTAGGTCTGATTATTATTGTTGGTGCATGGATTTGAGGATTTGA

Protein sequence

MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYSQRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMDICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYNGGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVYLGFDLQNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMSPEQMLEISRSDYYCWCMDLRI*
Homology
BLAST of CSPI05G28130 vs. ExPASy Swiss-Prot
Match: Q91560 (RNA exonuclease 4 OS=Xenopus laevis OX=8355 GN=rexo4 PE=2 SV=2)

HSP 1 Score: 104.0 bits (258), Expect = 3.2e-21
Identity = 60/156 (38.46%), Postives = 84/156 (53.85%), Query Frame = 0

Query: 104 IALACKYVGGGSDGSMDICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRD 163
           +A+ C+ VG G DG   I A+V +++     ++  YVKP   VT+YR   +GIRPE ++ 
Sbjct: 235 VAMDCEMVGVGMDGEESILARVSIVNLFGKCVYDKYVKPTERVTDYRTAVSGIRPEDVKK 294

Query: 164 AIPMKQVQKKIQEILYNGGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSK 223
             P K VQK++ EIL     + R LVG  V +DLK L + +P   IRDT  Y P  +  K
Sbjct: 295 GEPFKVVQKEVSEIL-----RGRTLVGHAVHNDLKILFLDHPKKAIRDTQKYKPFKQKVK 354

Query: 224 LCN-SLKYLAQVYLGFDLQNGIQDPYEECIVAMRLY 259
               SLK L +  L   +Q G     ++   AMRLY
Sbjct: 355 SGRPSLKLLCEKILNVKVQTGEHCSVQDAQAAMRLY 385

BLAST of CSPI05G28130 vs. ExPASy Swiss-Prot
Match: Q9GZR2 (RNA exonuclease 4 OS=Homo sapiens OX=9606 GN=REXO4 PE=1 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 1.6e-20
Identity = 62/186 (33.33%), Postives = 99/186 (53.23%), Query Frame = 0

Query: 84  LGIRDGSTVIDSGRTRGSG----AIALACKYVGGGSDGSMDICAKVCLIDEGENVIFYSY 143
           LG  +GS  +   + +  G    A+AL C+ VG G  G   + A+V ++++    ++  Y
Sbjct: 220 LGQSEGSVSLSLVKEQAFGGLTRALALDCEMVGVGPKGEESMAARVSIVNQYGKCVYDKY 279

Query: 144 VKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYNGGEKARILVGDCVEDDLKR 203
           VKP   VT+YR   +GIRPE+++    ++ VQK++ E+L     K RILVG  + +DLK 
Sbjct: 280 VKPTEPVTDYRTAVSGIRPENLKQGEELEVVQKEVAEML-----KGRILVGHALHNDLKV 339

Query: 204 LQIGYPSFMIRDTANYPPLMKSSKLCN-SLKYLAQVYLGFDLQNGIQDPYEECIVAMRLY 263
           L + +P   IRDT  Y P     K    SL+ L++  LG  +Q       ++   AMRLY
Sbjct: 340 LFLDHPKKKIRDTQKYKPFKSQVKSGRPSLRLLSEKILGLQVQQAEHCSIQDAQAAMRLY 399

Query: 264 KRMRSQ 265
             ++ +
Sbjct: 400 VMVKKE 400

BLAST of CSPI05G28130 vs. ExPASy Swiss-Prot
Match: O94375 (RNA exonuclease 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rex4 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.5e-20
Identity = 57/163 (34.97%), Postives = 97/163 (59.51%), Query Frame = 0

Query: 104 IALACKYVGGGSDGSMDICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRD 163
           IA+ C+ VG   D  M + A+V +++   +V++ +YV+P   VT++R   +G++  H+RD
Sbjct: 94  IAMDCEMVGVADD--MSVLARVSIVNYHGHVVYDTYVRPKEKVTDWRTWVSGVKSFHMRD 153

Query: 164 AIPMKQVQKKIQEILYNGGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSK 223
           A   ++VQ ++ +IL N     R+LVG  V +DLK L + +P  MIRDT+ +    K +K
Sbjct: 154 APSFEKVQAEVAKILDN-----RVLVGHAVHNDLKVLLLSHPRRMIRDTSRFSGYRKLAK 213

Query: 224 -LCNSLKYLAQVYLGFDLQNGIQDPYEECIVAMRLYKRMRSQV 266
                LK LA+V LG D+Q+G     ++    M LYK+++ ++
Sbjct: 214 GRTPGLKKLAEVILGRDIQSGQHSSVQDAQATMELYKKVKKEM 249

BLAST of CSPI05G28130 vs. ExPASy Swiss-Prot
Match: Q6FQA0 (RNA exonuclease 4 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=REX4 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 7.9e-20
Identity = 58/171 (33.92%), Postives = 96/171 (56.14%), Query Frame = 0

Query: 104 IALACKYVGGGSDGSMDICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRD 163
           IA+ C++VG G +G     A+V L++   NV+   +VKP   VT++R   +GI P+H+++
Sbjct: 96  IAMDCEFVGVGPEGKDSALARVSLVNFHGNVVLDIFVKPRETVTDWRTWVSGITPDHMKN 155

Query: 164 AIPMKQVQKKIQEILYNGGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMK--S 223
           A+  KQ Q+++ +IL     K +ILVG  V+ DL+ L + +P   + DTA + P  +  +
Sbjct: 156 AVSFKQAQQQLSDIL-----KDKILVGHAVKHDLEALMLSHPKSKVIDTARHLPFRQKYA 215

Query: 224 SKLCNSLKYLAQVYLGFDLQNGIQDPYEECIVAMRLYKRMR---SQVHRKE 270
                SLK LA+  L  D+Q+G     E+    M +YK  +    + HRK+
Sbjct: 216 KGKSPSLKKLAKEILNMDIQSGQHSSVEDARATMLIYKSAKQDFDKEHRKK 261

BLAST of CSPI05G28130 vs. ExPASy Swiss-Prot
Match: Q08237 (RNA exonuclease 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=REX4 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 7.9e-20
Identity = 59/183 (32.24%), Postives = 102/183 (55.74%), Query Frame = 0

Query: 91  TVIDSGRTRGSGA-IALACKYVGGGSDGSMDICAKVCLIDEGENVIFYSYVKPVTAVTNY 150
           T I+S +++  G  IA+ C++VG G +G     A++ +++   +V+   +VKP   V  +
Sbjct: 108 TRINSNKSKEIGKYIAMDCEFVGVGPEGKESALARISIVNYFGHVVLDEFVKPREKVVEW 167

Query: 151 RYETTGIRPEHIRDAIPMKQVQKKIQEILYNGGEKARILVGDCVEDDLKRLQIGYPSFMI 210
           R   +GI+PEH+++AI  K+ QKK  +IL     + RILVG  ++ DL+ L + +P  ++
Sbjct: 168 RTWVSGIKPEHMKNAITFKEAQKKTADIL-----EGRILVGHALKHDLEALMLSHPKSLL 227

Query: 211 RDTANYPPLMK--SSKLCNSLKYLAQVYLGFDLQNGIQDPYEECIVAMRLYKRMRSQ--- 268
           RDT+ + P  K  +     SLK L +  L   +Q G     E+    M LYK+ +++   
Sbjct: 228 RDTSRHLPFRKLYAKGKTPSLKKLTREVLKISIQEGEHSSVEDARATMLLYKKEKTEFEK 285

BLAST of CSPI05G28130 vs. ExPASy TrEMBL
Match: A0A6J1JZ30 (RNA exonuclease 4 OS=Cucurbita maxima OX=3661 GN=LOC111489566 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.2e-164
Identity = 276/316 (87.34%), Postives = 295/316 (93.35%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 60
           MRTSFHS HEPTCA+CKKHCRSLDSLREHLIGPLPKQECKN+F  RGCKFCLAI DSSYS
Sbjct: 29  MRTSFHSPHEPTCAVCKKHCRSLDSLREHLIGPLPKQECKNVFDMRGCKFCLAILDSSYS 88

Query: 61  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 120
           QRLHQERCQFS VNSGLLAR ANLGIRDGST ID GRTRGSG +ALACKYVGGG DGS+D
Sbjct: 89  QRLHQERCQFSCVNSGLLARLANLGIRDGSTAIDGGRTRGSGPVALACKYVGGG-DGSLD 148

Query: 121 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYN 180
           +CAKVCLIDE ENVIFYSYVKP   VTNYRYETTGIRPEH+RDA+P+KQVQKKIQ+ILYN
Sbjct: 149 VCAKVCLIDESENVIFYSYVKPTIPVTNYRYETTGIRPEHVRDAMPLKQVQKKIQDILYN 208

Query: 181 GGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVYLGFDL 240
           GGEKAR+LVG CVEDDLKRLQIGYPS MIRD ANYPPLMK+SKLCNSLKYLAQVYLGF++
Sbjct: 209 GGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIANYPPLMKTSKLCNSLKYLAQVYLGFEI 268

Query: 241 QNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMSPEQML 300
           QNG+QDPYEEC+  MRLYKRMRSQVHR E++PMASDPQNKNNFA WRE+EL+RMSPEQML
Sbjct: 269 QNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPMASDPQNKNNFAAWRESELERMSPEQML 328

Query: 301 EISRSDYYCWCMDLRI 317
           EISRSDYYCWCMDLRI
Sbjct: 329 EISRSDYYCWCMDLRI 343

BLAST of CSPI05G28130 vs. ExPASy TrEMBL
Match: A0A6J1FEK0 (RNA exonuclease 4 OS=Cucurbita moschata OX=3662 GN=LOC111444794 PE=3 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 3.5e-164
Identity = 275/316 (87.03%), Postives = 295/316 (93.35%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 60
           MRTSFHS HEPTCA+CKKHCRSLDSLREHLIGPLPKQECKN+F  RGCKFCLAI DSSYS
Sbjct: 29  MRTSFHSPHEPTCAVCKKHCRSLDSLREHLIGPLPKQECKNVFDMRGCKFCLAILDSSYS 88

Query: 61  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 120
           QRLHQERCQFS VNSGLLAR ANLGIRDGST ID GRTRGSG +ALACKYVGGG DGS+D
Sbjct: 89  QRLHQERCQFSCVNSGLLARLANLGIRDGSTAIDGGRTRGSGPVALACKYVGGG-DGSLD 148

Query: 121 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYN 180
           +CAKVCLIDE ENVIFYSYVKP+  VTNYRYE TGIRPEH+RDA+P+KQVQKKIQ+ILYN
Sbjct: 149 VCAKVCLIDESENVIFYSYVKPIIPVTNYRYEMTGIRPEHLRDAMPLKQVQKKIQDILYN 208

Query: 181 GGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVYLGFDL 240
           GGEKAR+LVG CVEDDLKRLQIGYPS MIRD ANYPPLMK+SKLCNSLKYLAQVYLGF++
Sbjct: 209 GGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIANYPPLMKTSKLCNSLKYLAQVYLGFEI 268

Query: 241 QNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMSPEQML 300
           QNG+QDPYEEC+  MRLYKRMRSQVHR E++PMASDPQNKNNFA WRE+EL+RMSPEQML
Sbjct: 269 QNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPMASDPQNKNNFAAWRESELERMSPEQML 328

Query: 301 EISRSDYYCWCMDLRI 317
           EISRSDYYCWCMDLRI
Sbjct: 329 EISRSDYYCWCMDLRI 343

BLAST of CSPI05G28130 vs. ExPASy TrEMBL
Match: A0A6J1BXL4 (RNA exonuclease 4 OS=Momordica charantia OX=3673 GN=LOC111006633 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.9e-160
Identity = 268/316 (84.81%), Postives = 291/316 (92.09%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 60
           MRTSFHS HEP CAICKKHCRSLDSLREHLIGPLPKQECKNIF TRGCKFCLAI DS YS
Sbjct: 29  MRTSFHSVHEPICAICKKHCRSLDSLREHLIGPLPKQECKNIFTTRGCKFCLAILDSPYS 88

Query: 61  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 120
           QRLHQERCQFS VNSGLLAR ANLGIRD S V+D GR R +G +ALACKYVGGG DGS+D
Sbjct: 89  QRLHQERCQFSGVNSGLLARLANLGIRDASIVVDGGRARATGTVALACKYVGGGGDGSLD 148

Query: 121 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYN 180
           ICAKVCLIDE ENVIFYSYVKP+  VTNYR+ETTGIRPEH+RDA+P+KQVQKKIQEILYN
Sbjct: 149 ICAKVCLIDETENVIFYSYVKPIIPVTNYRFETTGIRPEHLRDAMPLKQVQKKIQEILYN 208

Query: 181 GGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVYLGFDL 240
           GGEKAR+LVG  VEDDLKRLQIGYP  +IRDTANYPPLMK+SKLCNSLKYLAQVYLGFD+
Sbjct: 209 GGEKARVLVGHGVEDDLKRLQIGYP--IIRDTANYPPLMKTSKLCNSLKYLAQVYLGFDI 268

Query: 241 QNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMSPEQML 300
           QNGIQDPYE+C+ +MRLYKRMRSQVH+ E+YPMASDPQNKNNF IWR+NEL+RM+PE ML
Sbjct: 269 QNGIQDPYEDCVASMRLYKRMRSQVHKIEDYPMASDPQNKNNFGIWRQNELERMTPEHML 328

Query: 301 EISRSDYYCWCMDLRI 317
           +ISRSDYYCWC+DLRI
Sbjct: 329 DISRSDYYCWCLDLRI 342

BLAST of CSPI05G28130 vs. ExPASy TrEMBL
Match: A0A6J1BZE0 (RNA exonuclease 4 OS=Momordica charantia OX=3673 GN=LOC111006633 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.9e-160
Identity = 268/316 (84.81%), Postives = 291/316 (92.09%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 60
           MRTSFHS HEP CAICKKHCRSLDSLREHLIGPLPKQECKNIF TRGCKFCLAI DS YS
Sbjct: 32  MRTSFHSVHEPICAICKKHCRSLDSLREHLIGPLPKQECKNIFTTRGCKFCLAILDSPYS 91

Query: 61  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 120
           QRLHQERCQFS VNSGLLAR ANLGIRD S V+D GR R +G +ALACKYVGGG DGS+D
Sbjct: 92  QRLHQERCQFSGVNSGLLARLANLGIRDASIVVDGGRARATGTVALACKYVGGGGDGSLD 151

Query: 121 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYN 180
           ICAKVCLIDE ENVIFYSYVKP+  VTNYR+ETTGIRPEH+RDA+P+KQVQKKIQEILYN
Sbjct: 152 ICAKVCLIDETENVIFYSYVKPIIPVTNYRFETTGIRPEHLRDAMPLKQVQKKIQEILYN 211

Query: 181 GGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVYLGFDL 240
           GGEKAR+LVG  VEDDLKRLQIGYP  +IRDTANYPPLMK+SKLCNSLKYLAQVYLGFD+
Sbjct: 212 GGEKARVLVGHGVEDDLKRLQIGYP--IIRDTANYPPLMKTSKLCNSLKYLAQVYLGFDI 271

Query: 241 QNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMSPEQML 300
           QNGIQDPYE+C+ +MRLYKRMRSQVH+ E+YPMASDPQNKNNF IWR+NEL+RM+PE ML
Sbjct: 272 QNGIQDPYEDCVASMRLYKRMRSQVHKIEDYPMASDPQNKNNFGIWRQNELERMTPEHML 331

Query: 301 EISRSDYYCWCMDLRI 317
           +ISRSDYYCWC+DLRI
Sbjct: 332 DISRSDYYCWCLDLRI 345

BLAST of CSPI05G28130 vs. ExPASy TrEMBL
Match: A0A1S3C947 (RNA exonuclease 4 OS=Cucumis melo OX=3656 GN=LOC103498183 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.3e-147
Identity = 250/324 (77.16%), Postives = 285/324 (87.96%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 60
           MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQEC+NIFATRGCKFCLAI DSSYS
Sbjct: 29  MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECRNIFATRGCKFCLAILDSSYS 88

Query: 61  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 120
           QRLHQERCQFSPVNSGLLARFANLGIRDGST+IDSGRTRGSGAIALACKYVGGGSDGS+D
Sbjct: 89  QRLHQERCQFSPVNSGLLARFANLGIRDGSTIIDSGRTRGSGAIALACKYVGGGSDGSLD 148

Query: 121 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYN 180
           ICAKVCL+DEGENVIFYSYVKP+  VTNYRYE TG+RPEH+RDA+P+KQVQKKIQE L N
Sbjct: 149 ICAKVCLVDEGENVIFYSYVKPIIPVTNYRYEFTGVRPEHLRDAMPLKQVQKKIQEFLCN 208

Query: 181 GGE----------KARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKY 240
           G +          KARILVG  +++ L  LQI YPS MIRDTA YPPLMK+SKL NSL Y
Sbjct: 209 GEQMWKIRPGSTRKARILVGHGLQEYLTSLQIDYPSIMIRDTAKYPPLMKTSKLSNSLHY 268

Query: 241 LAQVYLGFDLQNGIQ-DPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWREN 300
           L Q YLG++++NG + + YEEC+  MRLY +M+SQVH++E YP+ASDP NK NFAIW++ 
Sbjct: 269 LTQTYLGYNMENGKKSEGYEECVATMRLYLKMKSQVHKREEYPLASDPPNKFNFAIWKQL 328

Query: 301 ELQRMSPEQMLEISRSDYYCWCMD 314
           EL++M+P+Q+L+ISRSDYYCWC+D
Sbjct: 329 ELEKMTPQQLLQISRSDYYCWCLD 352

BLAST of CSPI05G28130 vs. NCBI nr
Match: XP_011656270.1 (RNA exonuclease 4 [Cucumis sativus])

HSP 1 Score: 657.1 bits (1694), Expect = 7.5e-185
Identity = 314/316 (99.37%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 60
           MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS
Sbjct: 29  MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 88

Query: 61  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 120
           QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD
Sbjct: 89  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 148

Query: 121 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYN 180
           ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDA+P+KQVQKKIQEILYN
Sbjct: 149 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAMPVKQVQKKIQEILYN 208

Query: 181 GGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVYLGFDL 240
           GGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVYLGFDL
Sbjct: 209 GGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVYLGFDL 268

Query: 241 QNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMSPEQML 300
           QNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMSPEQML
Sbjct: 269 QNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMSPEQML 328

Query: 301 EISRSDYYCWCMDLRI 317
           EISRSDYYCWCMDLRI
Sbjct: 329 EISRSDYYCWCMDLRI 344

BLAST of CSPI05G28130 vs. NCBI nr
Match: XP_022993635.1 (RNA exonuclease 4-like [Cucurbita maxima])

HSP 1 Score: 589.0 bits (1517), Expect = 2.5e-164
Identity = 276/316 (87.34%), Postives = 295/316 (93.35%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 60
           MRTSFHS HEPTCA+CKKHCRSLDSLREHLIGPLPKQECKN+F  RGCKFCLAI DSSYS
Sbjct: 29  MRTSFHSPHEPTCAVCKKHCRSLDSLREHLIGPLPKQECKNVFDMRGCKFCLAILDSSYS 88

Query: 61  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 120
           QRLHQERCQFS VNSGLLAR ANLGIRDGST ID GRTRGSG +ALACKYVGGG DGS+D
Sbjct: 89  QRLHQERCQFSCVNSGLLARLANLGIRDGSTAIDGGRTRGSGPVALACKYVGGG-DGSLD 148

Query: 121 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYN 180
           +CAKVCLIDE ENVIFYSYVKP   VTNYRYETTGIRPEH+RDA+P+KQVQKKIQ+ILYN
Sbjct: 149 VCAKVCLIDESENVIFYSYVKPTIPVTNYRYETTGIRPEHVRDAMPLKQVQKKIQDILYN 208

Query: 181 GGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVYLGFDL 240
           GGEKAR+LVG CVEDDLKRLQIGYPS MIRD ANYPPLMK+SKLCNSLKYLAQVYLGF++
Sbjct: 209 GGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIANYPPLMKTSKLCNSLKYLAQVYLGFEI 268

Query: 241 QNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMSPEQML 300
           QNG+QDPYEEC+  MRLYKRMRSQVHR E++PMASDPQNKNNFA WRE+EL+RMSPEQML
Sbjct: 269 QNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPMASDPQNKNNFAAWRESELERMSPEQML 328

Query: 301 EISRSDYYCWCMDLRI 317
           EISRSDYYCWCMDLRI
Sbjct: 329 EISRSDYYCWCMDLRI 343

BLAST of CSPI05G28130 vs. NCBI nr
Match: XP_022938612.1 (RNA exonuclease 4-like [Cucurbita moschata])

HSP 1 Score: 587.4 bits (1513), Expect = 7.3e-164
Identity = 275/316 (87.03%), Postives = 295/316 (93.35%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 60
           MRTSFHS HEPTCA+CKKHCRSLDSLREHLIGPLPKQECKN+F  RGCKFCLAI DSSYS
Sbjct: 29  MRTSFHSPHEPTCAVCKKHCRSLDSLREHLIGPLPKQECKNVFDMRGCKFCLAILDSSYS 88

Query: 61  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 120
           QRLHQERCQFS VNSGLLAR ANLGIRDGST ID GRTRGSG +ALACKYVGGG DGS+D
Sbjct: 89  QRLHQERCQFSCVNSGLLARLANLGIRDGSTAIDGGRTRGSGPVALACKYVGGG-DGSLD 148

Query: 121 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYN 180
           +CAKVCLIDE ENVIFYSYVKP+  VTNYRYE TGIRPEH+RDA+P+KQVQKKIQ+ILYN
Sbjct: 149 VCAKVCLIDESENVIFYSYVKPIIPVTNYRYEMTGIRPEHLRDAMPLKQVQKKIQDILYN 208

Query: 181 GGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVYLGFDL 240
           GGEKAR+LVG CVEDDLKRLQIGYPS MIRD ANYPPLMK+SKLCNSLKYLAQVYLGF++
Sbjct: 209 GGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIANYPPLMKTSKLCNSLKYLAQVYLGFEI 268

Query: 241 QNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMSPEQML 300
           QNG+QDPYEEC+  MRLYKRMRSQVHR E++PMASDPQNKNNFA WRE+EL+RMSPEQML
Sbjct: 269 QNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPMASDPQNKNNFAAWRESELERMSPEQML 328

Query: 301 EISRSDYYCWCMDLRI 317
           EISRSDYYCWCMDLRI
Sbjct: 329 EISRSDYYCWCMDLRI 343

BLAST of CSPI05G28130 vs. NCBI nr
Match: XP_023551468.1 (RNA exonuclease 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 576.2 bits (1484), Expect = 1.7e-160
Identity = 270/316 (85.44%), Postives = 294/316 (93.04%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 60
           MRTSFHS HEPTCA+CKKHCRSLDSLREHLIGPLPKQECKN+F  RGCKFCLAI DSSYS
Sbjct: 29  MRTSFHSPHEPTCAVCKKHCRSLDSLREHLIGPLPKQECKNVFDMRGCKFCLAILDSSYS 88

Query: 61  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 120
           QR+HQERCQFS VNSGLLAR A+LGIRDGST ID GRTRGSG +ALACKYVGGG DGS+D
Sbjct: 89  QRVHQERCQFSCVNSGLLARLASLGIRDGSTAIDGGRTRGSGPVALACKYVGGG-DGSLD 148

Query: 121 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYN 180
           +CAKVCLIDE EN++FYSYVKP+  VTNYRYETTGIRPEH+RDA+P+KQVQKKIQ+ILYN
Sbjct: 149 VCAKVCLIDESENIVFYSYVKPIIPVTNYRYETTGIRPEHLRDAMPLKQVQKKIQDILYN 208

Query: 181 GGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVYLGFDL 240
           GGEKAR+LVG CVEDDLKRLQIGYPS MIRD ANYPPLMK+SKLCNSLKYLAQVYLGF++
Sbjct: 209 GGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIANYPPLMKTSKLCNSLKYLAQVYLGFEI 268

Query: 241 QNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMSPEQML 300
           QNG+QD Y +C+  MRLYKRMRSQVHR E++PMASDPQNKNNFA WRENEL+RMSPEQML
Sbjct: 269 QNGVQDLY-DCVGGMRLYKRMRSQVHRIEHHPMASDPQNKNNFAAWRENELERMSPEQML 328

Query: 301 EISRSDYYCWCMDLRI 317
           EISRSDYYCWCMDLRI
Sbjct: 329 EISRSDYYCWCMDLRI 342

BLAST of CSPI05G28130 vs. NCBI nr
Match: XP_022134351.1 (RNA exonuclease 4 isoform X1 [Momordica charantia])

HSP 1 Score: 573.2 bits (1476), Expect = 1.4e-159
Identity = 268/316 (84.81%), Postives = 291/316 (92.09%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 60
           MRTSFHS HEP CAICKKHCRSLDSLREHLIGPLPKQECKNIF TRGCKFCLAI DS YS
Sbjct: 29  MRTSFHSVHEPICAICKKHCRSLDSLREHLIGPLPKQECKNIFTTRGCKFCLAILDSPYS 88

Query: 61  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 120
           QRLHQERCQFS VNSGLLAR ANLGIRD S V+D GR R +G +ALACKYVGGG DGS+D
Sbjct: 89  QRLHQERCQFSGVNSGLLARLANLGIRDASIVVDGGRARATGTVALACKYVGGGGDGSLD 148

Query: 121 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYN 180
           ICAKVCLIDE ENVIFYSYVKP+  VTNYR+ETTGIRPEH+RDA+P+KQVQKKIQEILYN
Sbjct: 149 ICAKVCLIDETENVIFYSYVKPIIPVTNYRFETTGIRPEHLRDAMPLKQVQKKIQEILYN 208

Query: 181 GGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVYLGFDL 240
           GGEKAR+LVG  VEDDLKRLQIGYP  +IRDTANYPPLMK+SKLCNSLKYLAQVYLGFD+
Sbjct: 209 GGEKARVLVGHGVEDDLKRLQIGYP--IIRDTANYPPLMKTSKLCNSLKYLAQVYLGFDI 268

Query: 241 QNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMSPEQML 300
           QNGIQDPYE+C+ +MRLYKRMRSQVH+ E+YPMASDPQNKNNF IWR+NEL+RM+PE ML
Sbjct: 269 QNGIQDPYEDCVASMRLYKRMRSQVHKIEDYPMASDPQNKNNFGIWRQNELERMTPEHML 328

Query: 301 EISRSDYYCWCMDLRI 317
           +ISRSDYYCWC+DLRI
Sbjct: 329 DISRSDYYCWCLDLRI 342

BLAST of CSPI05G28130 vs. TAIR 10
Match: AT3G27970.1 (Exonuclease family protein )

HSP 1 Score: 449.1 bits (1154), Expect = 2.9e-126
Identity = 209/322 (64.91%), Postives = 256/322 (79.50%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 60
           M+ S+HS HEPTC +CKKHCRS +SLREHLIGPLPKQECKNIF+ RGC+FC+ I +S  S
Sbjct: 34  MKISYHSGHEPTCGVCKKHCRSFESLREHLIGPLPKQECKNIFSLRGCRFCMTILESPNS 93

Query: 61  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 120
           +R+HQERCQFS VNSGL  R A LG+RD   +ID   +R    +AL+CK VGGGSDGS+D
Sbjct: 94  RRIHQERCQFSSVNSGLTTRMAALGLRD-KAMIDYTSSRSPRVVALSCKMVGGGSDGSLD 153

Query: 121 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYN 180
           +CA+VC+ DE +NVIF++YVKP  AVT+YRYETTGIRPE++RDA+P+KQVQ+KIQE L N
Sbjct: 154 LCARVCITDESDNVIFHTYVKPSMAVTSYRYETTGIRPENLRDAMPLKQVQRKIQEFLCN 213

Query: 181 G---------GEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYL 240
           G         G KARILVG  ++ DL RLQ+ YPS MIRDTA YPPLMK+SKL NSLKYL
Sbjct: 214 GEPMWKIRPRGGKARILVGHGLDHDLDRLQLEYPSSMIRDTAKYPPLMKTSKLSNSLKYL 273

Query: 241 AQVYLGFDLQNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENEL 300
            Q YLG+D+  GIQDPYE+C+  MRLY RMR Q H+ E YP+A+D QN++N   WR++E 
Sbjct: 274 TQAYLGYDVHFGIQDPYEDCVATMRLYTRMRYQKHKIEAYPLAADAQNRSNQVAWRQSEA 333

Query: 301 QRMSPEQMLEISRSDYYCWCMD 314
           +RMSP++ML ISRSDYYCWC+D
Sbjct: 334 ERMSPDEMLSISRSDYYCWCLD 354

BLAST of CSPI05G28130 vs. TAIR 10
Match: AT5G40310.1 (Exonuclease family protein )

HSP 1 Score: 442.6 bits (1137), Expect = 2.7e-124
Identity = 208/322 (64.60%), Postives = 250/322 (77.64%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGPLPKQECKNIFATRGCKFCLAIFDSSYS 60
           MR S+HS HEPTC IC KHCRS DSLREHLIGPLPKQECKNIF+ RGC+FCL I +S  +
Sbjct: 25  MRISYHSVHEPTCGICNKHCRSFDSLREHLIGPLPKQECKNIFSIRGCRFCLTILESPNA 84

Query: 61  QRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGSMD 120
           +R+HQERCQ S V SGL+ R A LG+R+ ST ID   +R    +AL+CK VGGGSDGS+D
Sbjct: 85  RRIHQERCQLSNVTSGLMIRMAALGLRNNST-IDYTSSRSPRVVALSCKMVGGGSDGSLD 144

Query: 121 ICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEILYN 180
           +CA+VC+ DE ENV+F++YVKP   VTNYRYE TGIRPE++RDA+ +K  Q+K+QE L N
Sbjct: 145 LCARVCITDESENVVFHTYVKPTIPVTNYRYEMTGIRPENLRDAMRLKHAQRKVQEFLCN 204

Query: 181 G---------GEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYL 240
           G           KARILVG  +++ L  LQ+ Y S MIRDTA YPPLMKSSKL NSLKYL
Sbjct: 205 GEPMWKIRPRNGKARILVGHGLDNHLDSLQLEYSSSMIRDTAEYPPLMKSSKLSNSLKYL 264

Query: 241 AQVYLGFDLQNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENEL 300
            Q YLG+D+  GIQDPYE+C+  MRLY RMR Q HR E YP+ASD QN NNFA WR+NEL
Sbjct: 265 TQAYLGYDIHVGIQDPYEDCVATMRLYTRMRYQKHRAEAYPLASDTQNHNNFAAWRQNEL 324

Query: 301 QRMSPEQMLEISRSDYYCWCMD 314
           +RMSPE++L++SRSDYYCWC+D
Sbjct: 325 ERMSPEELLDLSRSDYYCWCLD 345

BLAST of CSPI05G28130 vs. TAIR 10
Match: AT2G48100.1 (Exonuclease family protein )

HSP 1 Score: 261.5 bits (667), Expect = 8.5e-70
Identity = 129/318 (40.57%), Postives = 198/318 (62.26%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGP--LPKQECKNIFATRGCKFCLAIFDSS 60
           M+ S+HS H+P C +C KHC+S +S+REHL  P  L K  CK IF  RGC  CL IF+ +
Sbjct: 36  MKISYHSLHQPRCGVCLKHCKSFESVREHLNVPDHLSKGNCKAIFTKRGCTLCLQIFEEA 95

Query: 61  YSQRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGS 120
           ++   H+ +C  SP              R+ S+ +   R +   A+AL C+ VGGG+DG+
Sbjct: 96  FALAEHKNKCHLSPPRP-----LGTSTQRNPSSSLAGSRLK---AMALDCEMVGGGADGT 155

Query: 121 MDICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEIL 180
           +D CA VCL+D+ ENVIF ++V+P+  VT+YR+E TG+  E ++D +P++ V++++   L
Sbjct: 156 IDQCASVCLVDDDENVIFSTHVQPLLPVTDYRHEITGLTKEDLKDGMPLEHVRERVFSFL 215

Query: 181 ---YNGGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVY 240
               N G    +LVG  +  D+  L++ YPS ++RDTA Y PLMK++ +  SLKYL + Y
Sbjct: 216 CGGQNDGAGRLLLVGHDLRHDMSCLKLEYPSHLLRDTAKYVPLMKTNLVSQSLKYLTKSY 275

Query: 241 LGFDLQNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMS 300
           LG+ +Q G  + YE+C+ AMRLYKRMR Q H      + S     N     ++++L++M+
Sbjct: 276 LGYKIQCGKHEVYEDCVSAMRLYKRMRDQEH------VCSGKAEGNGLNSRKQSDLEKMN 335

Query: 301 PEQMLEISRSDYYCWCMD 314
            E++ + S S+Y CWC+D
Sbjct: 336 AEELYQKSTSEYRCWCLD 339

BLAST of CSPI05G28130 vs. TAIR 10
Match: AT2G48100.2 (Exonuclease family protein )

HSP 1 Score: 261.5 bits (667), Expect = 8.5e-70
Identity = 129/318 (40.57%), Postives = 198/318 (62.26%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGP--LPKQECKNIFATRGCKFCLAIFDSS 60
           M+ S+HS H+P C +C KHC+S +S+REHL  P  L K  CK IF  RGC  CL IF+ +
Sbjct: 36  MKISYHSLHQPRCGVCLKHCKSFESVREHLNVPDHLSKGNCKAIFTKRGCTLCLQIFEEA 95

Query: 61  YSQRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGS 120
           ++   H+ +C  SP              R+ S+ +   R +   A+AL C+ VGGG+DG+
Sbjct: 96  FALAEHKNKCHLSPPRP-----LGTSTQRNPSSSLAGSRLK---AMALDCEMVGGGADGT 155

Query: 121 MDICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEIL 180
           +D CA VCL+D+ ENVIF ++V+P+  VT+YR+E TG+  E ++D +P++ V++++   L
Sbjct: 156 IDQCASVCLVDDDENVIFSTHVQPLLPVTDYRHEITGLTKEDLKDGMPLEHVRERVFSFL 215

Query: 181 ---YNGGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVY 240
               N G    +LVG  +  D+  L++ YPS ++RDTA Y PLMK++ +  SLKYL + Y
Sbjct: 216 CGGQNDGAGRLLLVGHDLRHDMSCLKLEYPSHLLRDTAKYVPLMKTNLVSQSLKYLTKSY 275

Query: 241 LGFDLQNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMS 300
           LG+ +Q G  + YE+C+ AMRLYKRMR Q H      + S     N     ++++L++M+
Sbjct: 276 LGYKIQCGKHEVYEDCVSAMRLYKRMRDQEH------VCSGKAEGNGLNSRKQSDLEKMN 335

Query: 301 PEQMLEISRSDYYCWCMD 314
            E++ + S S+Y CWC+D
Sbjct: 336 AEELYQKSTSEYRCWCLD 339

BLAST of CSPI05G28130 vs. TAIR 10
Match: AT2G48100.3 (Exonuclease family protein )

HSP 1 Score: 261.5 bits (667), Expect = 8.5e-70
Identity = 129/318 (40.57%), Postives = 198/318 (62.26%), Query Frame = 0

Query: 1   MRTSFHSHHEPTCAICKKHCRSLDSLREHLIGP--LPKQECKNIFATRGCKFCLAIFDSS 60
           M+ S+HS H+P C +C KHC+S +S+REHL  P  L K  CK IF  RGC  CL IF+ +
Sbjct: 36  MKISYHSLHQPRCGVCLKHCKSFESVREHLNVPDHLSKGNCKAIFTKRGCTLCLQIFEEA 95

Query: 61  YSQRLHQERCQFSPVNSGLLARFANLGIRDGSTVIDSGRTRGSGAIALACKYVGGGSDGS 120
           ++   H+ +C  SP              R+ S+ +   R +   A+AL C+ VGGG+DG+
Sbjct: 96  FALAEHKNKCHLSPPRP-----LGTSTQRNPSSSLAGSRLK---AMALDCEMVGGGADGT 155

Query: 121 MDICAKVCLIDEGENVIFYSYVKPVTAVTNYRYETTGIRPEHIRDAIPMKQVQKKIQEIL 180
           +D CA VCL+D+ ENVIF ++V+P+  VT+YR+E TG+  E ++D +P++ V++++   L
Sbjct: 156 IDQCASVCLVDDDENVIFSTHVQPLLPVTDYRHEITGLTKEDLKDGMPLEHVRERVFSFL 215

Query: 181 ---YNGGEKARILVGDCVEDDLKRLQIGYPSFMIRDTANYPPLMKSSKLCNSLKYLAQVY 240
               N G    +LVG  +  D+  L++ YPS ++RDTA Y PLMK++ +  SLKYL + Y
Sbjct: 216 CGGQNDGAGRLLLVGHDLRHDMSCLKLEYPSHLLRDTAKYVPLMKTNLVSQSLKYLTKSY 275

Query: 241 LGFDLQNGIQDPYEECIVAMRLYKRMRSQVHRKENYPMASDPQNKNNFAIWRENELQRMS 300
           LG+ +Q G  + YE+C+ AMRLYKRMR Q H      + S     N     ++++L++M+
Sbjct: 276 LGYKIQCGKHEVYEDCVSAMRLYKRMRDQEH------VCSGKAEGNGLNSRKQSDLEKMN 335

Query: 301 PEQMLEISRSDYYCWCMD 314
            E++ + S S+Y CWC+D
Sbjct: 336 AEELYQKSTSEYRCWCLD 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q915603.2e-2138.46RNA exonuclease 4 OS=Xenopus laevis OX=8355 GN=rexo4 PE=2 SV=2[more]
Q9GZR21.6e-2033.33RNA exonuclease 4 OS=Homo sapiens OX=9606 GN=REXO4 PE=1 SV=2[more]
O943753.5e-2034.97RNA exonuclease 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=2848... [more]
Q6FQA07.9e-2033.92RNA exonuclease 4 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / N... [more]
Q082377.9e-2032.24RNA exonuclease 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=55... [more]
Match NameE-valueIdentityDescription
A0A6J1JZ301.2e-16487.34RNA exonuclease 4 OS=Cucurbita maxima OX=3661 GN=LOC111489566 PE=3 SV=1[more]
A0A6J1FEK03.5e-16487.03RNA exonuclease 4 OS=Cucurbita moschata OX=3662 GN=LOC111444794 PE=3 SV=1[more]
A0A6J1BXL46.9e-16084.81RNA exonuclease 4 OS=Momordica charantia OX=3673 GN=LOC111006633 PE=3 SV=1[more]
A0A6J1BZE06.9e-16084.81RNA exonuclease 4 OS=Momordica charantia OX=3673 GN=LOC111006633 PE=3 SV=1[more]
A0A1S3C9472.3e-14777.16RNA exonuclease 4 OS=Cucumis melo OX=3656 GN=LOC103498183 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011656270.17.5e-18599.37RNA exonuclease 4 [Cucumis sativus][more]
XP_022993635.12.5e-16487.34RNA exonuclease 4-like [Cucurbita maxima][more]
XP_022938612.17.3e-16487.03RNA exonuclease 4-like [Cucurbita moschata][more]
XP_023551468.11.7e-16085.44RNA exonuclease 4-like [Cucurbita pepo subsp. pepo][more]
XP_022134351.11.4e-15984.81RNA exonuclease 4 isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G27970.12.9e-12664.91Exonuclease family protein [more]
AT5G40310.12.7e-12464.60Exonuclease family protein [more]
AT2G48100.18.5e-7040.57Exonuclease family protein [more]
AT2G48100.28.5e-7040.57Exonuclease family protein [more]
AT2G48100.38.5e-7040.57Exonuclease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013520Exonuclease, RNase T/DNA polymerase IIISMARTSM00479exoiiienduscoord: 102..267
e-value: 7.1E-15
score: 65.4
IPR013520Exonuclease, RNase T/DNA polymerase IIIPFAMPF00929RNase_Tcoord: 121..236
e-value: 8.4E-6
score: 26.4
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 101..281
e-value: 1.3E-32
score: 114.8
NoneNo IPR availablePANTHERPTHR12801:SF122OS02G0709000 PROTEINcoord: 1..313
NoneNo IPR availablePANTHERPTHR12801RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATEDcoord: 1..313
IPR037431RNA exonuclease 4, DEDDh 3'-5' exonuclease domainCDDcd06144REX4_likecoord: 104..259
e-value: 4.86743E-67
score: 205.057
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 116..263

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G28130.1CSPI05G28130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006364 rRNA processing
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003676 nucleic acid binding