CSPI05G26780 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G26780
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4-like
LocationChr5: 25582762 .. 25583711 (-)
RNA-Seq ExpressionCSPI05G26780
SyntenyCSPI05G26780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAACCATCCAACTTTCATTTGGAACAATAATCCACTTTATAAGAAAATGGTGGTCAGCGAAGAAATTACATTGAGATGCTTCGACATAAAAGGCATAGTCCTCCACGTCATCACTGGACGATGGTTCATGGTTTTTGCTTCACTCCTAATCATGGCAATGTCTGGATCAATGTACATGTTCGGTTTGTATTCGAGCTACATTAAATCGATTTTGGGTTACGACCAAACGACGTTGACTTTGTTGAGTTTTATTAAGGATTTGGGTGCAAACGTTGGCGTTTTATCTGGTCTCATTAACGAGGTTACGTTATCGTGGGTGATTCTATTAATTGGTGTTGTTTTGAATTTCTTTGGTTACTTTATGATTTGGCTTAGTGTTACAGGTCGGATCACCACCCAAAAAGTTTGGCAAATGTGTCTCTATATTTGTATTGGAGGAAGTGCTCAAAATACGAGTTGTACAGGTACTTGTGTCAATAACTTTCCCGGAAAGGCGTGGCTTTGTTCTCGGTAAGTCTTTATTAAAATGAGAATGGCTAATTAGACAATACTTCTAATTCAAAGGTTAAAACCCAATTAGGTATTTTAAAGGGATACGTCGGGCTAAGCGGCGCCATAATCACACAGCTCTTCCATGCATTTTACGGCGGCGACACCAAATCCTTAATTCTCCTCATCGGATGGCTTCCGGCTGCAATTTCCTTCGCATTCTTACGAACAGTTTGTATCATGAAAGTCATCAGACAGCCAAATGAGCTCAAAGTTTTCTACAATTTCCTCTACATTTCTCTTGTTCTCGCCGGATTCCTCATGCTAATGATCATTGTACAAACGCAAAACAGAGTTCACTCAAAACCAATATGGTGGCAGCGCTGCCGCCATCGTCGTCCTTCTCCTCCTCCCACTCCCCATCGTCATTATCGAAGAATACAATCTCCGGAAACTTAA

mRNA sequence

CACAACCATCCAACTTTCATTTGGAACAATAATCCACTTTATAAGAAAATGGTGGTCAGCGAAGAAATTACATTGAGATGCTTCGACATAAAAGGCATAGTCCTCCACGTCATCACTGGACGATGGTTCATGGTTTTTGCTTCACTCCTAATCATGGCAATGTCTGGATCAATGTACATGTTCGGTTTGTATTCGAGCTACATTAAATCGATTTTGGGTTACGACCAAACGACGTTGACTTTGTTGAGTTTTATTAAGGATTTGGGTGCAAACGTTGGCGTTTTATCTGGTCTCATTAACGAGGTCGGATCACCACCCAAAAAGTTTGGCAAATGTGTCTCTATATTTGTATTGGAGGAAGTGCTCAAAATACGAGTTGTACAGGGATACGTCGGGCTAAGCGGCGCCATAATCACACAGCTCTTCCATGCATTTTACGGCGGCGACACCAAATCCTTAATTCTCCTCATCGGATGGCTTCCGGCTGCAATTTCCTTCGCATTCTTACGAACAGTTTGTATCATGAAAGTCATCAGACAGCCAAATGAGCTCAAAGTTTTCTACAATTTCCTCTACATTTCTCTTGTTCTCGCCGGATTCCTCATGCTAATGATCATTGTACAAACGCAAAACAGAGTTCACTCAAAACCAATATGGTGGCAGCGCTGCCGCCATCGTCGTCCTTCTCCTCCTCCCACTCCCCATCGTCATTATCGAAGAATACAATCTCCGGAAACTTAA

Coding sequence (CDS)

ATGGTGGTCAGCGAAGAAATTACATTGAGATGCTTCGACATAAAAGGCATAGTCCTCCACGTCATCACTGGACGATGGTTCATGGTTTTTGCTTCACTCCTAATCATGGCAATGTCTGGATCAATGTACATGTTCGGTTTGTATTCGAGCTACATTAAATCGATTTTGGGTTACGACCAAACGACGTTGACTTTGTTGAGTTTTATTAAGGATTTGGGTGCAAACGTTGGCGTTTTATCTGGTCTCATTAACGAGGTCGGATCACCACCCAAAAAGTTTGGCAAATGTGTCTCTATATTTGTATTGGAGGAAGTGCTCAAAATACGAGTTGTACAGGGATACGTCGGGCTAAGCGGCGCCATAATCACACAGCTCTTCCATGCATTTTACGGCGGCGACACCAAATCCTTAATTCTCCTCATCGGATGGCTTCCGGCTGCAATTTCCTTCGCATTCTTACGAACAGTTTGTATCATGAAAGTCATCAGACAGCCAAATGAGCTCAAAGTTTTCTACAATTTCCTCTACATTTCTCTTGTTCTCGCCGGATTCCTCATGCTAATGATCATTGTACAAACGCAAAACAGAGTTCACTCAAAACCAATATGGTGGCAGCGCTGCCGCCATCGTCGTCCTTCTCCTCCTCCCACTCCCCATCGTCATTATCGAAGAATACAATCTCCGGAAACTTAA

Protein sequence

MVVSEEITLRCFDIKGIVLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTLLSFIKDLGANVGVLSGLINEVGSPPKKFGKCVSIFVLEEVLKIRVVQGYVGLSGAIITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTVCIMKVIRQPNELKVFYNFLYISLVLAGFLMLMIIVQTQNRVHSKPIWWQRCRHRRPSPPPTPHRHYRRIQSPET*
Homology
BLAST of CSPI05G26780 vs. ExPASy TrEMBL
Match: A0A0A0KRJ3 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G622537 PE=4 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.4e-98
Identity = 198/230 (86.09%), Postives = 201/230 (87.39%), Query Frame = 0

Query: 1   MVVSEEITLRCFDIKGIVLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQ 60
           MVVSEEITLRCFDIKGIVLH+ITGRWFMVFASLLIMAMS SMYMFGLYSSYIKSILGYDQ
Sbjct: 1   MVVSEEITLRCFDIKGIVLHIITGRWFMVFASLLIMAMSESMYMFGLYSSYIKSILGYDQ 60

Query: 61  TTLTLLSFIKDLGANVGVLSGLINEVGSPPKKFGKCVSIFVLEEVLKIRVVQGYVGLSGA 120
           TTLTLLSFIKDLGANVGVLSGLINEV                       ++ GYVGLSGA
Sbjct: 61  TTLTLLSFIKDLGANVGVLSGLINEV----------------------TLLWGYVGLSGA 120

Query: 121 IITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTVCIMKVIRQPNELKVFYNFLYISLV 180
           IITQLFHAFYGGDTKSLILLIGWLPAAIS AFLRTV IMKVIRQPNELKVFYNFLYISL 
Sbjct: 121 IITQLFHAFYGGDTKSLILLIGWLPAAISIAFLRTVRIMKVIRQPNELKVFYNFLYISLG 180

Query: 181 LAGFLMLMIIVQTQNRVHSKPIWWQRCRHRRPSPPPTPHRHYRRIQSPET 231
           LAGFLMLMIIVQTQNRVHSKPIWWQRCRHRRPSPPPTPHRHYRRIQS ET
Sbjct: 181 LAGFLMLMIIVQTQNRVHSKPIWWQRCRHRRPSPPPTPHRHYRRIQSLET 208

BLAST of CSPI05G26780 vs. ExPASy TrEMBL
Match: A0A6J1GZI8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111458291 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 3.1e-61
Identity = 143/233 (61.37%), Postives = 159/233 (68.24%), Query Frame = 0

Query: 11  CFDIKGIVLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTLLSFIK 70
           C DI  I +HVITGRWF+VFASLLIMA +G+ YMF LYSS IKS+LGYDQTTL LLSF K
Sbjct: 10  CSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQTTLNLLSFFK 69

Query: 71  DLGANVGVLSGLINEVGSP----------------------------PKKFGKCVSI--- 130
           DLGANVGVLSGLINEV  P                            PK +  C+ I   
Sbjct: 70  DLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVWQMCLYICIG 129

Query: 131 ------------------FVLEEVLKIRVVQGYVGLSGAIITQLFHAFYGGDTKSLILLI 190
                             F     + + +++GYVGLSGAIITQLFHAFYGGDTKSLILLI
Sbjct: 130 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTKSLILLI 189

Query: 191 GWLPAAISFAFLRTVCIMKVIRQPNELKVFYNFLYISLVLAGFLMLMIIVQTQ 195
           GWLPAAISFAFLRT+ IMKVIRQPNELKVFYNFLYISL LAGFLMLMIIV+++
Sbjct: 190 GWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESK 242

BLAST of CSPI05G26780 vs. ExPASy TrEMBL
Match: A0A6J1K186 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489721 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 4.1e-61
Identity = 142/233 (60.94%), Postives = 159/233 (68.24%), Query Frame = 0

Query: 11  CFDIKGIVLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTLLSFIK 70
           C DI  I +HVITGRWF+VFASLLIMA +G+ YMF LYSS IKS+LGYDQTTL LLSF K
Sbjct: 10  CSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQTTLNLLSFFK 69

Query: 71  DLGANVGVLSGLINEVGSP----------------------------PKKFGKCVSI--- 130
           DLGANVGVLSGLINEV  P                            PK +  C+ I   
Sbjct: 70  DLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTGKISAPKVWQMCLYICIG 129

Query: 131 ------------------FVLEEVLKIRVVQGYVGLSGAIITQLFHAFYGGDTKSLILLI 190
                             F     + + +++GYVGLSGAIITQLFHAFYGGDTKSLILLI
Sbjct: 130 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTKSLILLI 189

Query: 191 GWLPAAISFAFLRTVCIMKVIRQPNELKVFYNFLYISLVLAGFLMLMIIVQTQ 195
           GWLPAAISFAFLRT+ IMKVIRQPNELKVFYNFLYISL LAGFLMLMII++++
Sbjct: 190 GWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESK 242

BLAST of CSPI05G26780 vs. ExPASy TrEMBL
Match: A0A5D3CFW3 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002400 PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 4.5e-60
Identity = 142/233 (60.94%), Postives = 158/233 (67.81%), Query Frame = 0

Query: 11  CFDIKGIVLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTLLSFIK 70
           C DI  I +HVITGRWF+VFASLLIMA +G+ YMFGLYSS IKS+LGYDQTTL LLSF K
Sbjct: 10  CSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFK 69

Query: 71  DLGANVGVLSGLINEVGSP----------------------------PKKFGKCVSI--- 130
           DLGANVGVLSGLINEV  P                            PK +  C+ I   
Sbjct: 70  DLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVWQMCLYICIG 129

Query: 131 ------------------FVLEEVLKIRVVQGYVGLSGAIITQLFHAFYGGDTKSLILLI 190
                             F     + + +++GYVGLSGAIITQLFHAFYG DTKSLILLI
Sbjct: 130 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLI 189

Query: 191 GWLPAAISFAFLRTVCIMKVIRQPNELKVFYNFLYISLVLAGFLMLMIIVQTQ 195
           GWLPAAISFA LRT+ IMKVIRQPNELKVFYNFLYISL LAGFLMLMIIV+++
Sbjct: 190 GWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESK 242

BLAST of CSPI05G26780 vs. ExPASy TrEMBL
Match: A0A1S3BG09 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 4.5e-60
Identity = 142/233 (60.94%), Postives = 158/233 (67.81%), Query Frame = 0

Query: 11  CFDIKGIVLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTLLSFIK 70
           C DI  I +HVITGRWF+VFASLLIMA +G+ YMFGLYSS IKS+LGYDQTTL LLSF K
Sbjct: 10  CSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFK 69

Query: 71  DLGANVGVLSGLINEVGSP----------------------------PKKFGKCVSI--- 130
           DLGANVGVLSGLINEV  P                            PK +  C+ I   
Sbjct: 70  DLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVWQMCLYICIG 129

Query: 131 ------------------FVLEEVLKIRVVQGYVGLSGAIITQLFHAFYGGDTKSLILLI 190
                             F     + + +++GYVGLSGAIITQLFHAFYG DTKSLILLI
Sbjct: 130 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLI 189

Query: 191 GWLPAAISFAFLRTVCIMKVIRQPNELKVFYNFLYISLVLAGFLMLMIIVQTQ 195
           GWLPAAISFA LRT+ IMKVIRQPNELKVFYNFLYISL LAGFLMLMIIV+++
Sbjct: 190 GWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESK 242

BLAST of CSPI05G26780 vs. NCBI nr
Match: KAE8648844.1 (hypothetical protein Csa_007724 [Cucumis sativus])

HSP 1 Score: 377.9 bits (969), Expect = 6.4e-101
Identity = 200/230 (86.96%), Postives = 207/230 (90.00%), Query Frame = 0

Query: 1   MVVSEEITLRCFDIKGIVLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQ 60
           MVVSEEITLRCFDIKGIVLH+ITGRWFMVFASLLIMAMS SMYMFGLYSSYIKSILGYDQ
Sbjct: 1   MVVSEEITLRCFDIKGIVLHIITGRWFMVFASLLIMAMSESMYMFGLYSSYIKSILGYDQ 60

Query: 61  TTLTLLSFIKDLGANVGVLSGLINEVGSPPKKFGKCVSIFVLEEVLKIRVVQGYVGLSGA 120
           TTLTLLSFIKDLGANVGVLSGLINEV +                  ++ +++GYVGLSGA
Sbjct: 61  TTLTLLSFIKDLGANVGVLSGLINEVKT------------------QLGILKGYVGLSGA 120

Query: 121 IITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTVCIMKVIRQPNELKVFYNFLYISLV 180
           IITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTV IMKVIRQPNELKVFYNFLYISLV
Sbjct: 121 IITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTVRIMKVIRQPNELKVFYNFLYISLV 180

Query: 181 LAGFLMLMIIVQTQNRVHSKPIWWQRCRHRRPSPPPTPHRHYRRIQSPET 231
           LAGFLMLMIIVQTQNRVHSKPIWWQRCRHRRPSPPPTPHRHYRRIQS ET
Sbjct: 181 LAGFLMLMIIVQTQNRVHSKPIWWQRCRHRRPSPPPTPHRHYRRIQSLET 212

BLAST of CSPI05G26780 vs. NCBI nr
Match: XP_031741159.1 (uncharacterized protein LOC101205029 [Cucumis sativus] >KAE8648846.1 hypothetical protein Csa_008243 [Cucumis sativus])

HSP 1 Score: 250.0 bits (637), Expect = 2.0e-62
Identity = 149/243 (61.32%), Postives = 168/243 (69.14%), Query Frame = 0

Query: 1   MVVSEEITLRCFDIKGIVLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQ 60
           MVVS+  + RCFDIK IV+HVI GRWF+VFASLLIMA+SGS +MFGLYS  IKS+LGY+Q
Sbjct: 1   MVVSKG-SSRCFDIKNIVVHVIIGRWFVVFASLLIMAVSGSTFMFGLYSGDIKSVLGYNQ 60

Query: 61  TTLTLLSFIKDLGANVGVLSGLINEVGSP----------------------------PKK 120
           TTL LLSF KDLGANVG+LSGLINEV  P                            PK 
Sbjct: 61  TTLNLLSFFKDLGANVGILSGLINEVTPPWVILLIGALMNFFGYFMIWLSVTRRIFAPKV 120

Query: 121 FGKCVSI---------------------FVLEEVLKIRVVQGYVGLSGAIITQLFHAFYG 180
           +  C+ I                     F     + + +++GYVGLSGAIITQLFHAFYG
Sbjct: 121 WQMCLYICMGGSSQNFANTGALVTCVNNFPERRGIVLGLLKGYVGLSGAIITQLFHAFYG 180

Query: 181 GDTKSLILLIGWLPAAISFAFLRTVCIMKVIRQPNELKVFYNFLYISLVLAGFLMLMIIV 195
           GDTKS ILLIGWLPAAIS AFLRTV IMKVIRQPNELKVFYNFLYISL+LAGFLMLMIIV
Sbjct: 181 GDTKSFILLIGWLPAAISLAFLRTVRIMKVIRQPNELKVFYNFLYISLLLAGFLMLMIIV 240

BLAST of CSPI05G26780 vs. NCBI nr
Match: XP_031741165.1 (uncharacterized protein LOC101205265 [Cucumis sativus] >KAE8648845.1 hypothetical protein Csa_009229 [Cucumis sativus])

HSP 1 Score: 248.4 bits (633), Expect = 5.9e-62
Identity = 145/238 (60.92%), Postives = 166/238 (69.75%), Query Frame = 0

Query: 6   EITLRCFDIKGIVLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTL 65
           E TLRCFDIK IV++VITGRWF+VFASLLIMA+ G+ ++FGLYSS IKS LGY+QTTL L
Sbjct: 5   EGTLRCFDIKSIVVYVITGRWFVVFASLLIMAVFGTTFLFGLYSSDIKSALGYNQTTLNL 64

Query: 66  LSFIKDLGANVGVLSGLINE---------VGSPPKKFG---------------------- 125
           LSF KDLGANVG+LSGLINE         +G+    FG                      
Sbjct: 65  LSFFKDLGANVGILSGLINEFMPPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCF 124

Query: 126 ------------------KCVSIFVLEEVLKIRVVQGYVGLSGAIITQLFHAFYGGDTKS 185
                              CV+ F     + + +++GY+GLSGAIITQLFHAFYGGDTKS
Sbjct: 125 YICMGGSSQSFANTGSMVTCVNNFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKS 184

Query: 186 LILLIGWLPAAISFAFLRTVCIMKVIRQPNELKVFYNFLYISLVLAGFLMLMIIVQTQ 195
           LIL IGWLPAAISFAFLRTV IMKVIRQPNELKVFYNFLYISL+LAGFLMLMIIVQ++
Sbjct: 185 LILFIGWLPAAISFAFLRTVRIMKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSK 242

BLAST of CSPI05G26780 vs. NCBI nr
Match: KAG6601185.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 246.9 bits (629), Expect = 1.7e-61
Identity = 143/233 (61.37%), Postives = 160/233 (68.67%), Query Frame = 0

Query: 11  CFDIKGIVLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTLLSFIK 70
           C DI  I +H+ITGRWF+VFASLLIMA +G+ YMFGLYSS IKS+LGYDQTTL LLSF K
Sbjct: 10  CSDISSIAVHIITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFK 69

Query: 71  DLGANVGVLSGLINEVGSP----------------------------PKKFGKCVSI--- 130
           DLGANVGVLSGLINEV  P                            PK +  C+ I   
Sbjct: 70  DLGANVGVLSGLINEVTPPWVVLSIGAIMNFFGYFMIWLAVTQKIAAPKVWQMCLYICIG 129

Query: 131 ------------------FVLEEVLKIRVVQGYVGLSGAIITQLFHAFYGGDTKSLILLI 190
                             F     + + +++GYVGLSGAIITQLFHAFYGGDTKSLILLI
Sbjct: 130 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTKSLILLI 189

Query: 191 GWLPAAISFAFLRTVCIMKVIRQPNELKVFYNFLYISLVLAGFLMLMIIVQTQ 195
           GWLPAAISFAFLRT+ IMKVIRQPNELKVFYNFLYISL LAGFLMLMIIV+++
Sbjct: 190 GWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESK 242

BLAST of CSPI05G26780 vs. NCBI nr
Match: XP_023535761.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 245.4 bits (625), Expect = 5.0e-61
Identity = 144/236 (61.02%), Postives = 160/236 (67.80%), Query Frame = 0

Query: 8   TLRCFDIKGIVLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTLLS 67
           T  C DI  I +HVITGRWF+VFASLLIMA +G+ YMF LYSS IKS+LGYDQTTL LLS
Sbjct: 7   TSGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQTTLNLLS 66

Query: 68  FIKDLGANVGVLSGLINEVGSP----------------------------PKKFGKCVSI 127
           F KDLGANVGVLSGLINEV  P                            PK +  C+ I
Sbjct: 67  FFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTQKISAPKVWQMCLYI 126

Query: 128 ---------------------FVLEEVLKIRVVQGYVGLSGAIITQLFHAFYGGDTKSLI 187
                                F     + + +++GYVGLSGAIITQLFHAFYGGDTKSLI
Sbjct: 127 CIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTKSLI 186

Query: 188 LLIGWLPAAISFAFLRTVCIMKVIRQPNELKVFYNFLYISLVLAGFLMLMIIVQTQ 195
           LLIGWLPAAISFAFLRT+ IMKVIRQPNELKVFYNFLYISL LAGFLMLMIIV+++
Sbjct: 187 LLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESK 242

BLAST of CSPI05G26780 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 199.9 bits (507), Expect = 2.2e-51
Identity = 117/227 (51.54%), Postives = 142/227 (62.56%), Query Frame = 0

Query: 14  IKGIVLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTLLSFIKDLG 73
           +K + + ++TGRWFM F SLLIM+ +G+ YMFG+YS  IK  LGYDQTTL LLSF KDLG
Sbjct: 10  MKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLG 69

Query: 74  ANVGVLSGLINE-------------------------------------------VGSPP 133
           ANVGVL+GL+NE                                           VG+  
Sbjct: 70  ANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANS 129

Query: 134 KKFGK------CVSIFVLEEVLKIRVVQGYVGLSGAIITQLFHAFYGGDTKSLILLIGWL 192
           + F        CV  F     + + +++GYVGLSGAIITQL+ AFYG DTK LIL+IGWL
Sbjct: 130 QSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWL 189

BLAST of CSPI05G26780 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 2.9e-43
Identity = 108/226 (47.79%), Postives = 128/226 (56.64%), Query Frame = 0

Query: 18  VLHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTLLSFIKDLGANVG 77
           ++H   GRWFMVFAS LIMA +G+ Y+FG YS  IKS LGYDQTTL LL F KDLGANVG
Sbjct: 10  LIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVG 69

Query: 78  VLSGLINEV-------------------------------------------GSPPKKFG 137
           VLSGLI EV                                           G+  + F 
Sbjct: 70  VLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFA 129

Query: 138 K------CVSIFVLEEVLKIRVVQGYVGLSGAIITQLFHAFYGGDTKSLILLIGWLPAAI 195
                  CV  F     + + +++GYVGLSGAI TQL+ A YG D+KSLILLI WLPAA+
Sbjct: 130 NTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAV 189

BLAST of CSPI05G26780 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 79.7 bits (195), Expect = 3.4e-15
Identity = 64/229 (27.95%), Postives = 100/229 (43.67%), Query Frame = 0

Query: 25  RWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTLLSFIKDLGANVGVLSGLI- 84
           +W    AS+ I + SG+ Y FG+YSS +KS   YDQ+TL  +S  KD+GANVG+LSGL  
Sbjct: 7   KWVAAAASIWIQSFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILSGLFY 66

Query: 85  ----------------------------------------NEVGSPPKKF--------GK 144
                                                     +  PP           G 
Sbjct: 67  TAVASRKSGNGGFFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFMFFAGH 126

Query: 145 CVSIFVLEEVL------------KIRVVQGYVGLSGAIITQLFHAFYGGDTKSLILLIGW 193
           C   F    V+             + +++GY+GLSGAI+ Q++H F GGD ++ ILL+  
Sbjct: 127 CQPFFNTAIVVTAVRNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYILLLAV 186

BLAST of CSPI05G26780 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 9.8e-15
Identity = 66/246 (26.83%), Postives = 107/246 (43.50%), Query Frame = 0

Query: 21  VITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTLLSFIKDLGANVGVLS 80
           ++  +W  + AS+ I   +G  Y FG+YS+ +KS   YDQ+TL  +S  KD+G NVGVLS
Sbjct: 5   ILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVLS 64

Query: 81  GLI--------------NEVGSP----------------------------PKKFGKCVS 140
           GL+                 G P                            P     C+ 
Sbjct: 65  GLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCLF 124

Query: 141 IFVLEEVL---------------------KIRVVQGYVGLSGAIITQLFHAFYGGDTKSL 200
           +F+  + L                      + +++G+VGLSGA++ QL+     GD K+ 
Sbjct: 125 MFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKTF 184

Query: 201 ILLIGWLPAAISFAFLRTVCIMKVIRQPNELKVFYNFLYISLVLAGFLMLMIIVQTQNRV 204
           ILL+  +P+ +S   +  V + K     +E K       +SL++A +LM+ II+++    
Sbjct: 185 ILLLAIVPSLLSVLVMPLVRVYKT-STVDEKKHLDGLSTLSLIIAAYLMITIILKS---T 244

BLAST of CSPI05G26780 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.4e-13
Identity = 59/239 (24.69%), Postives = 102/239 (42.68%), Query Frame = 0

Query: 19  LHVITGRWFMVFASLLIMAMSGSMYMFGLYSSYIKSILGYDQTTLTLLSFIKDLGANVGV 78
           + ++  +W  + AS+ I   SG+ Y FG+YS+ +KS   YDQ+TL  +S  KD+GAN GV
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 79  LSGLINEVGSPPKKFGK------------------------------------------- 138
            SGL+    +  +  G+                                           
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 139 -CVSIFVLEEV---------------------LKIRVVQGYVGLSGAIITQLFHAFYGGD 193
            C+ +F+  +                        + +++G++GLSGAI+ QL+     GD
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KRJ31.4e-9886.09Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G62253... [more]
A0A6J1GZI83.1e-6161.37protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1K1864.1e-6160.94protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489... [more]
A0A5D3CFW34.5e-6060.94Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BG094.5e-6060.94protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 ... [more]
Match NameE-valueIdentityDescription
KAE8648844.16.4e-10186.96hypothetical protein Csa_007724 [Cucumis sativus][more]
XP_031741159.12.0e-6261.32uncharacterized protein LOC101205029 [Cucumis sativus] >KAE8648846.1 hypothetica... [more]
XP_031741165.15.9e-6260.92uncharacterized protein LOC101205265 [Cucumis sativus] >KAE8648845.1 hypothetica... [more]
KAG6601185.11.7e-6161.37Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023535761.15.0e-6161.02protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G39210.12.2e-5151.54Major facilitator superfamily protein [more]
AT2G28120.12.9e-4347.79Major facilitator superfamily protein [more]
AT2G34350.13.4e-1527.95Nodulin-like / Major Facilitator Superfamily protein [more]
AT1G18940.19.8e-1526.83Nodulin-like / Major Facilitator Superfamily protein [more]
AT1G74780.12.4e-1324.69Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 25..86
e-value: 2.7E-21
score: 76.2
coord: 95..199
e-value: 1.7E-21
score: 76.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 209..230
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 11..89
NoneNo IPR availablePANTHERPTHR21576:SF122MFS TRANSPORTERcoord: 107..196
NoneNo IPR availablePANTHERPTHR21576:SF122MFS TRANSPORTERcoord: 11..89
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 107..196

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G26780.1CSPI05G26780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane