CSPI05G26590 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G26590
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
LocationChr5: 25412042 .. 25413656 (+)
RNA-Seq ExpressionCSPI05G26590
SyntenyCSPI05G26590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATTAAAAAGCCCAATTCTCTCACCTTCCTAACACACAAATATTTCCACAAAGAGAACAAACTATTGTTCCGATTGGGGCGCGGCGGTGGTGGCAAAGTGGAAGTCGTGATCTTACGACATGGCTGAGAACGAAGAAAATATTATGAGAAACCCTAAATTGCCAAGTTTTGTGCGAAATAATCGACAGAAATTAAATAATGTGGATCAAAAGAAAGAAGAAATTAATCTACCTGAGACGAAGTCTGAGGTAGTCACTATTCCGGCACCGGAAGTGGTTCCCCCTCCGCCGATTAAGGCTGAGATCAAGCACCATGGGAAGTTTTCACATCCGGCAATCGTGTGGTCGGTATGATTATTCCTTCTCCCATTCTCATTATATCTCTCCTTTTATGGAGAAGTTTTTTTTTTTGTCTTAGTTCTTGGTTTCTAAAACATTGCATTTTAGTTTAGTTTTGGGTTTGATTTTAATTCATACTATCACAAAAACCTTAACGTCATTTTGCCTTTGAAAACAAAAAATGTCACATTTTAAAGTTTAGAGATTAAATTAAAATCAAACTTAAAATTTTAAATCGAAAGAAATGTGATATTTAATCACATTGATAATAAACATAAAAATTAGAGACTTTTTAGCTCAGTATGTCTATGACTTATTAAACACAATTTTAAGTTTAGGTTTTATAACCAAAGTGAAAATAAATTATTACTTCGTTGTAAAGTGAGATTCTATTATCATCCCAAAATGTAGGTTTTTAAATTTAGTTCATCTCGTTTGTTAATTATTTTTTTTTTTTTTAACTTTGTTGATTTTCATATAGAAATTTATTCTTTCGATTTGTTTAGAGATCATAAACATTTTTTATCGATTAAATAGTTAATTAATTTCTCACGAAAAAAAATCCTTCATCAAATATGTTTGTTTAGTACATTAATCAACCCTATATATAAGAATTCAAAAAAAAAAAATATGTTTGTTTGTTTCAAGTGTGTATGTGTAATAGTTTTTTTTGTTAAAAAATCAATGAAGAAAATAAATTGGTGAGAAACGAAGATGTGAATGTATTCTCTGTCTCCGTTCCCGTGAAGCTCTAGCTCGGAAAAATAGACTTATATGTACTGTAACTCCGCTGTCCATGAAGGGTTACTCATTAGAATTCTTTGTCAAACAGTTTTCGATGATCAAAATTCATGAAGTATTATTACTACCAAACAAATTGCAGTTTCAACTAACAATAATAAATAATAAAGTAATAAGTAGCAATTAAGAATGCAGCTTTTCTATTGTATATATATGATTTATAAAAAGAATTAATTTTGTTTTAATGGTAATTCATTTCATCTACTTTCAGTTATGTGCTTTAGGAGGATTTTTCATCATAAGGTTTGCTTGGAATAAATGGAAACCTAAAGACGACGAAACGAAAAAAAAATAATATCTCAACTCCATATTGGAAGCAATCCATAATTAATTTTAAGCATTTCAAATTTAATGCTCTTTGTTACTCTATATTTACTGATTTGTAATGTGATTGCTCATTTATTTATAAATTTGAGCCATGATAATAAGGCTTTTAATGATATTCTTTGATTACTATGGTTGTTGTAATAATTAC

mRNA sequence

CAATTAAAAAGCCCAATTCTCTCACCTTCCTAACACACAAATATTTCCACAAAGAGAACAAACTATTGTTCCGATTGGGGCGCGGCGGTGGTGGCAAAGTGGAAGTCGTGATCTTACGACATGGCTGAGAACGAAGAAAATATTATGAGAAACCCTAAATTGCCAAGTTTTGTGCGAAATAATCGACAGAAATTAAATAATGTGGATCAAAAGAAAGAAGAAATTAATCTACCTGAGACGAAGTCTGAGGTAGTCACTATTCCGGCACCGGAAGTGGTTCCCCCTCCGCCGATTAAGGCTGAGATCAAGCACCATGGGAAGTTTTCACATCCGGCAATCGTGTGGTCGTTATGTGCTTTAGGAGGATTTTTCATCATAAGGTTTGCTTGGAATAAATGGAAACCTAAAGACGACGAAACGAAAAAAAAATAATATCTCAACTCCATATTGGAAGCAATCCATAATTAATTTTAAGCATTTCAAATTTAATGCTCTTTGTTACTCTATATTTACTGATTTGTAATGTGATTGCTCATTTATTTATAAATTTGAGCCATGATAATAAGGCTTTTAATGATATTCTTTGATTACTATGGTTGTTGTAATAATTAC

Coding sequence (CDS)

ATGGCTGAGAACGAAGAAAATATTATGAGAAACCCTAAATTGCCAAGTTTTGTGCGAAATAATCGACAGAAATTAAATAATGTGGATCAAAAGAAAGAAGAAATTAATCTACCTGAGACGAAGTCTGAGGTAGTCACTATTCCGGCACCGGAAGTGGTTCCCCCTCCGCCGATTAAGGCTGAGATCAAGCACCATGGGAAGTTTTCACATCCGGCAATCGTGTGGTCGTTATGTGCTTTAGGAGGATTTTTCATCATAAGGTTTGCTTGGAATAAATGGAAACCTAAAGACGACGAAACGAAAAAAAAATAA

Protein sequence

MAENEENIMRNPKLPSFVRNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIKAEIKHHGKFSHPAIVWSLCALGGFFIIRFAWNKWKPKDDETKKK*
Homology
BLAST of CSPI05G26590 vs. ExPASy TrEMBL
Match: A0A0A0KRF3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G616350 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.3e-51
Identity = 101/103 (98.06%), Postives = 102/103 (99.03%), Query Frame = 0

Query: 1   MAENEENIMRNPKLPSFVRNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIKA 60
           MAENEENIMRNPKLPSFVRNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIKA
Sbjct: 1   MAENEENIMRNPKLPSFVRNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIKA 60

Query: 61  EIKHHGKFSHPAIVWSLCALGGFFIIRFAWNKWKPKDDETKKK 104
           EIKHHGKFSHPAIVWSLCALGGFFII+FAW KWKPKDDETKKK
Sbjct: 61  EIKHHGKFSHPAIVWSLCALGGFFIIKFAWKKWKPKDDETKKK 103

BLAST of CSPI05G26590 vs. ExPASy TrEMBL
Match: A0A1S3BGK5 (uncharacterized protein LOC103489415 OS=Cucumis melo OX=3656 GN=LOC103489415 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 5.4e-29
Identity = 72/103 (69.90%), Postives = 83/103 (80.58%), Query Frame = 0

Query: 1   MAENEENIMRNPKLPSFV-RNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIK 60
           MAEN+E+I++NPKLPSFV  NNRQK+  VDQKKEE N PE K +VVTIPA  VV PPP+K
Sbjct: 1   MAENKEHIIQNPKLPSFVLNNNRQKV--VDQKKEETNPPE-KPDVVTIPASGVVSPPPVK 60

Query: 61  AEIKHHGKFSHPAIVWSLCALGGFFIIRFAWNKWKPKDDETKK 103
           AE K  GKFSHPA++W L ALGGF II +AW KW+P DDETKK
Sbjct: 61  AEFKFPGKFSHPAVLWPLSALGGFLIIWWAWQKWRPDDDETKK 100

BLAST of CSPI05G26590 vs. ExPASy TrEMBL
Match: A0A5D3CFU1 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002190 PE=4 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 3.1e-16
Identity = 53/76 (69.74%), Postives = 62/76 (81.58%), Query Frame = 0

Query: 1  MAENEENIMRNPKLPSFV-RNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIK 60
          MAEN+E+I++NPKLPSFV  NNRQK+  VDQKKEE N PE K +VVTIPA  VV PPP+K
Sbjct: 1  MAENKEHIIQNPKLPSFVLNNNRQKV--VDQKKEETNPPE-KPDVVTIPASGVVSPPPVK 60

Query: 61 AEIKHHGKFSHPAIVW 76
          AE K  GKFSHPA++W
Sbjct: 61 AEFKFPGKFSHPAVLW 73

BLAST of CSPI05G26590 vs. ExPASy TrEMBL
Match: A0A6J1J8Z1 (uncharacterized protein LOC111483672 OS=Cucurbita maxima OX=3661 GN=LOC111483672 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.2e-15
Identity = 50/101 (49.50%), Postives = 62/101 (61.39%), Query Frame = 0

Query: 1   MAENEENIMRNPKLPSFVRNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIKA 60
           M E  + +  NPK+  F   NR K  + D ++E   +P  K  VVT+P  EV PPPP+ A
Sbjct: 1   MVEYRDIVPTNPKVAWFWPRNRDK--SGDNEEEPSPMPPQKPNVVTLPGSEVAPPPPLAA 60

Query: 61  EIKHHGKFSHPAIVWSLCALGGFFIIRFAWNKWKPKDDETK 102
           E K  GKFSHPAI WSL ALGGF I R+A  +WK  +DE K
Sbjct: 61  ECKRPGKFSHPAIRWSLYALGGFIIARWALKRWKSGEDEGK 99

BLAST of CSPI05G26590 vs. ExPASy TrEMBL
Match: A0A6J1GYG5 (uncharacterized protein LOC111458630 OS=Cucurbita moschata OX=3662 GN=LOC111458630 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 6.4e-14
Identity = 50/105 (47.62%), Postives = 64/105 (60.95%), Query Frame = 0

Query: 1   MAENEENIMRNPKLP---SFVRNNRQKLNNV-DQKKEEINLPETKSEVVTIPAPEVVPPP 60
           M +N + +  NP+L    S+ RN    LN + D +++   LP  K  +VT+P  EV PPP
Sbjct: 17  MVDNRDIVPTNPQLTWSWSWSRN----LNKLGDNEQQTSPLPPRKPNLVTLPGSEVAPPP 76

Query: 61  PIKAEIKHHGKFSHPAIVWSLCALGGFFIIRFAWNKWKPKDDETK 102
           P+ AE K  GKFSHPAI W L ALGGF I R+A  KWK  +DE K
Sbjct: 77  PLTAECKRPGKFSHPAIRWPLYALGGFLIARWALKKWKSGEDEGK 117

BLAST of CSPI05G26590 vs. NCBI nr
Match: XP_011655848.1 (uncharacterized protein LOC101213355 [Cucumis sativus] >KGN52215.1 hypothetical protein Csa_008804 [Cucumis sativus])

HSP 1 Score: 211.1 bits (536), Expect = 4.7e-51
Identity = 101/103 (98.06%), Postives = 102/103 (99.03%), Query Frame = 0

Query: 1   MAENEENIMRNPKLPSFVRNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIKA 60
           MAENEENIMRNPKLPSFVRNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIKA
Sbjct: 1   MAENEENIMRNPKLPSFVRNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIKA 60

Query: 61  EIKHHGKFSHPAIVWSLCALGGFFIIRFAWNKWKPKDDETKKK 104
           EIKHHGKFSHPAIVWSLCALGGFFII+FAW KWKPKDDETKKK
Sbjct: 61  EIKHHGKFSHPAIVWSLCALGGFFIIKFAWKKWKPKDDETKKK 103

BLAST of CSPI05G26590 vs. NCBI nr
Match: XP_008446792.1 (PREDICTED: uncharacterized protein LOC103489415 [Cucumis melo])

HSP 1 Score: 136.7 bits (343), Expect = 1.1e-28
Identity = 72/103 (69.90%), Postives = 83/103 (80.58%), Query Frame = 0

Query: 1   MAENEENIMRNPKLPSFV-RNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIK 60
           MAEN+E+I++NPKLPSFV  NNRQK+  VDQKKEE N PE K +VVTIPA  VV PPP+K
Sbjct: 1   MAENKEHIIQNPKLPSFVLNNNRQKV--VDQKKEETNPPE-KPDVVTIPASGVVSPPPVK 60

Query: 61  AEIKHHGKFSHPAIVWSLCALGGFFIIRFAWNKWKPKDDETKK 103
           AE K  GKFSHPA++W L ALGGF II +AW KW+P DDETKK
Sbjct: 61  AEFKFPGKFSHPAVLWPLSALGGFLIIWWAWQKWRPDDDETKK 100

BLAST of CSPI05G26590 vs. NCBI nr
Match: KAA0034675.1 (uncharacterized protein E6C27_scaffold65G006940 [Cucumis melo var. makuwa] >TYK09226.1 uncharacterized protein E5676_scaffold475G002190 [Cucumis melo var. makuwa])

HSP 1 Score: 94.4 bits (233), Expect = 6.4e-16
Identity = 53/76 (69.74%), Postives = 62/76 (81.58%), Query Frame = 0

Query: 1  MAENEENIMRNPKLPSFV-RNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIK 60
          MAEN+E+I++NPKLPSFV  NNRQK+  VDQKKEE N PE K +VVTIPA  VV PPP+K
Sbjct: 1  MAENKEHIIQNPKLPSFVLNNNRQKV--VDQKKEETNPPE-KPDVVTIPASGVVSPPPVK 60

Query: 61 AEIKHHGKFSHPAIVW 76
          AE K  GKFSHPA++W
Sbjct: 61 AEFKFPGKFSHPAVLW 73

BLAST of CSPI05G26590 vs. NCBI nr
Match: XP_022985696.1 (uncharacterized protein LOC111483672 [Cucurbita maxima])

HSP 1 Score: 92.4 bits (228), Expect = 2.4e-15
Identity = 50/101 (49.50%), Postives = 62/101 (61.39%), Query Frame = 0

Query: 1   MAENEENIMRNPKLPSFVRNNRQKLNNVDQKKEEINLPETKSEVVTIPAPEVVPPPPIKA 60
           M E  + +  NPK+  F   NR K  + D ++E   +P  K  VVT+P  EV PPPP+ A
Sbjct: 1   MVEYRDIVPTNPKVAWFWPRNRDK--SGDNEEEPSPMPPQKPNVVTLPGSEVAPPPPLAA 60

Query: 61  EIKHHGKFSHPAIVWSLCALGGFFIIRFAWNKWKPKDDETK 102
           E K  GKFSHPAI WSL ALGGF I R+A  +WK  +DE K
Sbjct: 61  ECKRPGKFSHPAIRWSLYALGGFIIARWALKRWKSGEDEGK 99

BLAST of CSPI05G26590 vs. NCBI nr
Match: XP_022957161.1 (uncharacterized protein LOC111458630 [Cucurbita moschata])

HSP 1 Score: 86.7 bits (213), Expect = 1.3e-13
Identity = 50/105 (47.62%), Postives = 64/105 (60.95%), Query Frame = 0

Query: 1   MAENEENIMRNPKLP---SFVRNNRQKLNNV-DQKKEEINLPETKSEVVTIPAPEVVPPP 60
           M +N + +  NP+L    S+ RN    LN + D +++   LP  K  +VT+P  EV PPP
Sbjct: 17  MVDNRDIVPTNPQLTWSWSWSRN----LNKLGDNEQQTSPLPPRKPNLVTLPGSEVAPPP 76

Query: 61  PIKAEIKHHGKFSHPAIVWSLCALGGFFIIRFAWNKWKPKDDETK 102
           P+ AE K  GKFSHPAI W L ALGGF I R+A  KWK  +DE K
Sbjct: 77  PLTAECKRPGKFSHPAIRWPLYALGGFLIARWALKKWKSGEDEGK 117

BLAST of CSPI05G26590 vs. TAIR 10
Match: AT3G52230.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast outer membrane, chloroplast thylakoid membrane, chloroplast, chloroplast envelope; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; Has 29 Blast hits to 29 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 3; Fungi - 0; Plants - 26; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 48.1 bits (113), Expect = 4.9e-06
Identity = 22/62 (35.48%), Postives = 36/62 (58.06%), Query Frame = 0

Query: 43  EVVTIPAPEVVPPPPIKAEIK-HHGKFSHPAIVWSLCALGGFFIIRFAWNKWKPKDDETK 102
           E VT P        PIK E +   G+ S+  I+W + ALGGF ++++AW +W  +++ + 
Sbjct: 61  ETVTFPYNPPKSAEPIKFEAEPSSGRTSNSVILWQVYALGGFLVLKWAWARWNERNERSD 120

Query: 103 KK 104
           KK
Sbjct: 121 KK 122

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KRF32.3e-5198.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G616350 PE=4 SV=1[more]
A0A1S3BGK55.4e-2969.90uncharacterized protein LOC103489415 OS=Cucumis melo OX=3656 GN=LOC103489415 PE=... [more]
A0A5D3CFU13.1e-1669.74Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1J8Z11.2e-1549.50uncharacterized protein LOC111483672 OS=Cucurbita maxima OX=3661 GN=LOC111483672... [more]
A0A6J1GYG56.4e-1447.62uncharacterized protein LOC111458630 OS=Cucurbita moschata OX=3662 GN=LOC1114586... [more]
Match NameE-valueIdentityDescription
XP_011655848.14.7e-5198.06uncharacterized protein LOC101213355 [Cucumis sativus] >KGN52215.1 hypothetical ... [more]
XP_008446792.11.1e-2869.90PREDICTED: uncharacterized protein LOC103489415 [Cucumis melo][more]
KAA0034675.16.4e-1669.74uncharacterized protein E6C27_scaffold65G006940 [Cucumis melo var. makuwa] >TYK0... [more]
XP_022985696.12.4e-1549.50uncharacterized protein LOC111483672 [Cucurbita maxima][more]
XP_022957161.11.3e-1347.62uncharacterized protein LOC111458630 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G52230.14.9e-0635.48unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..41
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..39
NoneNo IPR availablePANTHERPTHR36374:SF1OS01G0969000 PROTEINcoord: 11..103
NoneNo IPR availablePANTHERPTHR36374OS01G0969000 PROTEINcoord: 11..103

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G26590.1CSPI05G26590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane