CSPI05G26580 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G26580
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionmRNA (guanine-N(7)-)-methyltransferase
LocationChr5: 25404870 .. 25409645 (+)
RNA-Seq ExpressionCSPI05G26580
SyntenyCSPI05G26580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAAGTTCTGAAATAGTAACGACTTGCATAAACCCTTTGAAGTGAGGAAGAGCCGAAATTCAAATCTTGGAAACGGAGGGTAAAAAAAGGCGCGAACCATCGTTCCGGCGACCGGAAATTATCAACTCAGAACTAAAATGAGTCAACTCGTCCCGCCAAGAGGCGAGTGGACTCACCACCGCCTTTGCGATTTTGCAAAGACCGCCCTCATCAAGATCTTCTCGCACCCCTACGTCACGGTTCGCTGTTTAACTCAATCTTCAATTTACGTATATGATTTTGGTGCTTGTATGTAAGATGTAATTAGGTTTTGTTTATTAAGGTTTGTGATTTGTATTGCGCGCGAGGAGCGGATGCGGAGAAATGGGACGAAACTCAAATCAGTCACTACATTGGAATTGGTATCTAGGTTTTTCTCTTCTCTCTTTTGAAACTGTAGATTATTTTCCTTTTTTTTTTCACGGAGGAATTTTCGTTGAGTTGTTTTTTTGAAAGAATTGAAATTATTTAGATGAATCCACTTCTGGAATCGGCCAAATGCGAGAGGCATGGGAGAGTCAGAAGAAGGCTTACACTGCTGAATTCTTTGAAGTGGATCCTTGTGTGGTGAGTTTTTGCTGCTAATTCTTTAGTTTCCTTTTCAATTACTATGAATTTCCAAATCGAAGAAATACCTGTTTTCAATCAAACTACAGAGTTATATTTAACTTGAAAGTACTGGATAAATCATATATGGACAGATTGATCATTGTTTCCTTCCTCTATTAATTCTGGACAAATCAAAATGTTAACCGGTGTAATGTTTAATGGTCCAAAGGAATTTATTGTAAATTTGGTTCATGGAAAATAAACGAATCGTCCTATATAAGTTTTTTGGTCACAAAATCACTTTGAAGTATTTCAGAGAAATCTGTCTATGAGAAGTAGTTCATGTGAAACATTATTATCTTCAAAATTTTCCTTTTATTGGATGCTACTTCTTGAACAAGATTGTCCTAAGCGCTCTGTCATGACTGTACAATGGAACTGATTCTTAGATGGATAGATACTAAAACATTTGAAATTCATTCCATGTAGATTTCTTCTTTTCTTTTCCTTTTTTTAATAAAAAAACAAAGGATAGCCTGCAAAATAACACAAAGGCCATACAAAAAAACCTAAGGGACAAGGGGATAAGGCATCCCCTTTCCCGATGGAAGGTTCACAAAATAACTTGTGTTGAGCATTTCATATAGATTTATTCCTAGCACTTCTCTATACTTGTAAAATGGGAAAGGGATAAAGTGGTCGGTGGAGGATTTTTGGGAGATGGGATGCTCCTTTGTTTAGCAAAGCACTATGAGTTTTGCATTTATTGCAAAAATATTTCTTATCGCTGAGTGTGGCCTAAGATCTCCACTTTTTTCCACTTATTCCTCATGCTGCGGTTTATATAACATTGTTTTAATGATATGAAATATTTGGTTAATGCTGTCTAAGAACCAAATAAGGCTGTTGAACAAGGTTTCCCTAATCAGTTATTTGAGTTATTGAAAGGAGCCAAAAGAGAAATTGGGCCATTGTACAAGTGCTCAAAATTTGAGCACCGCTGTACTATCATTATTGCTTTTGCAAGGAATAAATAGGGCAAGAAACAATCACACAAGACTACATGAGCTCAGGAAGTGGAAGTGAGAACGGATTGAACTATTATATCTATGTCTTATATCTTCTGTGATGGATAGGCTGTAACTATTAGTCAAAAGCGCACTTGCTCAGAGTGAGAAAATAAATAACTGAAATGTTGCAACCTGCTTTTCAAAAAATGGCATTCTCTTTGATAATGCCTGAAATCATATTTTTACTTAAAAGAATTGTGGAGTAGAATGCAAATCTCCCTTTGTTTTTTTCCCTTTCCATCTTGCCATCACTTGTTAAACTTCAGTAGAAAAGAAATGGATGATACTTCACTTCACACAGTAGCAAATGCATGCTTTAATTTTATTTTAATCATTTAAGTATCAAGACCACTTTCATCCTGATCAAACGCTAATTCATTGGGTCCTCTATTTTGAATTACAGATTTGATTATAATGCAAGTGTTAAACTTATAATACCAATGTAAAGTAAGTTATCAGATAGCATGTAACATTTAAAACTGAGACACGTCAACTTTGTTGCCCGGTATTTTATTTTTGTTATGTTCCTGCTGATTGTATTCTGTTACCACAGGAAAATATCGAAACTCAGTTGAAAGACAAAACGGAAACAGTTGATCGTGTCTGCTGCTTGCAGCATCTTCAGGTCCTACATCTGGAATTTCTTCTTGTTTTTTTGTGGATTAGAGGAGAGAATGGATCTTGACTTTCATATATTAGACGTATGTGCTGAATATGAAGGAAATTTCTGCAGATGTGTTTTGAAACCGAAGAGAGAGCAAGGAGACTCTTACATAATGTGTCAGCATTGCTTAAACCTGGGGGCTATTTTTTTGGGATTACTCCAGACTCGTCTACAATATGGTGAGGAATAACAATCAAATCCAGTCTTTTTAGGAGGGAGTTCATTGTTATTTTCATATTTTAGAAGTTCGTTCTGTAGGAGTTAGTTTGAAATATTAGATTCTCTTACTTTTAAAGGGCAAAGTATCAGAAGAATGTCGAAGCATACCACAATAGGAGTGCCGGTATGAAGCCTAATATAGTACCCAATTGCATTAGATCGGAAAGCTACATGATCTCCTTTGAAGTGGAGGAAGAGAAGTACGTAGAATTTCCTAGTTTTTGAATATTTCTTTTCCTTTGGATTGTTTTAATGAATGGTTTTTGTTATAACCGAAGATATTGAGTATTTACAGGTTCCCTTTGTTTGGGAAGAAATATCAGCTGAAATTTGCAAATGACCCCTCTGCTGAGACTAATTGTTTAGTTCATTTTCCAAGCTTGATCAGGTTTAGTTATTTATTATCCATCTCCAACCACATCGTGTTTCAGTTCATAGACATCATCCTTCTAAGTTCCATCAAATAAAACTGCTTATGAAACCAAAATTTCCTCTCATGAATAAATTTTTTCCTTATATGCTTTTACTGACATATATGACTATGTAATCATCCAAAATATCTGAATGAATTTATCCAAATGGTGGCTATAACTCACTCAGGTTGGCTAGAGAAGCAGGTCTTGAGTATATCGAGATTCAAAATTTGACCGAATTCTTCGATGATCACAGGTTTTTGCCGTCTTTCTTAAATTCCTTGTTTTCTCTAACTCATGAAAAGCTCACCAACTCGTGTTTTTCCTGGCCCGGCTGATTATTTTGTCCTTGTTTTGATTGCCGCAAAAAGAGCACAATTGGCAGATATGCTCATGAATTTTGGTCAAAACATTCTGGATCCCAGAGGGAGACTTCTTCCTCGATCATACGATGTACTGGGTGAGAAAGGATTCCCCCCCTACCCACAGTCTATTTTTATTTTTGCAATAAGATATTATTCATATGTTTTTATGTAACTTTCTTTACAGGTCTCTACACAACATTCATATTTCAGAAACCAGATCCAGACATCACTCCACCAATTATGACCCCATTGTTGCCAGAAACTATTTATGATCATGAAGAGGCAAGTTTCTGTCTTTAGAAATTCTTTACAAATATAGTATAAAGAAAAGAAAAGAAAATAATTAACTCAAAACTCTCCAAGTTACTTATATGCAATACTCTGGTAAGGGGACGTTTCACAACCAACATGTACTTGAGTTCGGTGGGTAAATAGAACACACTTGCTGTCTGCCTGAACCAACAATTGAGGAGGTTTATTATCTCGCTATTCATATCAACTCACATTTATCACCTCATTATTTATGTTAACTTATATTTCTCACGTCGTTATTTGCATCAACTTCAACTCTCAAGGAAACTTATAATAATTAACAACTTAACTCAACTCTCAAGGAAACTTATACTTGACGTCTAAATTTGGTGCATGAGACTTGTCACAAGCCCATTGTTTCGTAGAGTCATTAAAACTTTTAGACATACAGTCTCCTTATAATGTTGACGGTTACAATGATAGTGCTAGATCTCAAGAATTGCAAGCACAACATGACTTGGGAGATAGAATGTTGACGGTCACTTTCATAATGAAGCTATTTTCAAATATAGCAAAATAAACCAAAATATTTATAAAATATAGCAAAATATCACCGTCTATTTTACGATACCACGATAAACTATTAATCTAATCTTGGTCTATTATGGAGTAGATTATAATATTTTATTGTATTTATAAATATTTTAAGAAGTTTTATCCTTTAAAACAATTTTCTATCACTACATGTTTCTAATTAAGGTTGGTGATCTGTTTGGTCATCTGGCTTTGTTATTTGGTTTTGTAGACAGAGTGGCTGGGGAATGTGTGGCGAGATGAAGAAAGAAGTGTACAGGCAGAGCATGTTTCTGGTCAGGTTCCAGTTCCTGTGCCTGTTCCGGTTCCTATTCCGGTTGCGGTTCCAGTTCCGGTTTCAGTTACCGTTCAGGTTCCTGTTGTGGTACCACTCACCGTCCCTGGGCTTGGCAAGATTAGTGAACAGAAGGGGATTTTGGGTCCTGGCCCTGCAGATTTACGTTTTCCGGAGGCTCTTTAGCTTTAGCTTTACCTTTGTTTGTTATAAATCAATGCTCGTTTTGAGCTATGATCCTAGTTTATAACTAATTATTATTTATTAAGCTTAATTTGGTGGCAATGTAAAGAAAAATTTTGCATGATAGATGAGAAGATCGATGCATTTATTGAG

mRNA sequence

CCAAAGTTCTGAAATAGTAACGACTTGCATAAACCCTTTGAAGTGAGGAAGAGCCGAAATTCAAATCTTGGAAACGGAGGGTAAAAAAAGGCGCGAACCATCGTTCCGGCGACCGGAAATTATCAACTCAGAACTAAAATGAGTCAACTCGTCCCGCCAAGAGGCGAGTGGACTCACCACCGCCTTTGCGATTTTGCAAAGACCGCCCTCATCAAGATCTTCTCGCACCCCTACGTCACGGTTTGTGATTTGTATTGCGCGCGAGGAGCGGATGCGGAGAAATGGGACGAAACTCAAATCAGTCACTACATTGGAATTGATGAATCCACTTCTGGAATCGGCCAAATGCGAGAGGCATGGGAGAGTCAGAAGAAGGCTTACACTGCTGAATTCTTTGAAGTGGATCCTTGTGTGGAAAATATCGAAACTCAGTTGAAAGACAAAACGGAAACAGTTGATCGTGTCTGCTGCTTGCAGCATCTTCAGATGTGTTTTGAAACCGAAGAGAGAGCAAGGAGACTCTTACATAATGTGTCAGCATTGCTTAAACCTGGGGGCTATTTTTTTGGGATTACTCCAGACTCGTCTACAATATGGGCAAAGTATCAGAAGAATGTCGAAGCATACCACAATAGGAGTGCCGGTATGAAGCCTAATATAGTACCCAATTGCATTAGATCGGAAAGCTACATGATCTCCTTTGAAGTGGAGGAAGAGAAGTTCCCTTTGTTTGGGAAGAAATATCAGCTGAAATTTGCAAATGACCCCTCTGCTGAGACTAATTGTTTAGTTCATTTTCCAAGCTTGATCAGGTTGGCTAGAGAAGCAGGTCTTGAGTATATCGAGATTCAAAATTTGACCGAATTCTTCGATGATCACAGAGCACAATTGGCAGATATGCTCATGAATTTTGGTCAAAACATTCTGGATCCCAGAGGGAGACTTCTTCCTCGATCATACGATGTACTGGGTCTCTACACAACATTCATATTTCAGAAACCAGATCCAGACATCACTCCACCAATTATGACCCCATTGTTGCCAGAAACTATTTATGATCATGAAGAGACAGAGTGGCTGGGGAATGTGTGGCGAGATGAAGAAAGAAGTGTACAGGCAGAGCATGTTTCTGGTCAGGTTCCAGTTCCTGTGCCTGTTCCGGTTCCTATTCCGGTTGCGGTTCCAGTTCCGGTTTCAGTTACCGTTCAGGTTCCTGTTGTGGTACCACTCACCGTCCCTGGGCTTGGCAAGATTAGTGAACAGAAGGGGATTTTGGGTCCTGGCCCTGCAGATTTACGTTTTCCGGAGGCTCTTTAGCTTTAGCTTTACCTTTGTTTGTTATAAATCAATGCTCGTTTTGAGCTATGATCCTAGTTTATAACTAATTATTATTTATTAAGCTTAATTTGGTGGCAATGTAAAGAAAAATTTTGCATGATAGATGAGAAGATCGATGCATTTATTGAG

Coding sequence (CDS)

ATGAGTCAACTCGTCCCGCCAAGAGGCGAGTGGACTCACCACCGCCTTTGCGATTTTGCAAAGACCGCCCTCATCAAGATCTTCTCGCACCCCTACGTCACGGTTTGTGATTTGTATTGCGCGCGAGGAGCGGATGCGGAGAAATGGGACGAAACTCAAATCAGTCACTACATTGGAATTGATGAATCCACTTCTGGAATCGGCCAAATGCGAGAGGCATGGGAGAGTCAGAAGAAGGCTTACACTGCTGAATTCTTTGAAGTGGATCCTTGTGTGGAAAATATCGAAACTCAGTTGAAAGACAAAACGGAAACAGTTGATCGTGTCTGCTGCTTGCAGCATCTTCAGATGTGTTTTGAAACCGAAGAGAGAGCAAGGAGACTCTTACATAATGTGTCAGCATTGCTTAAACCTGGGGGCTATTTTTTTGGGATTACTCCAGACTCGTCTACAATATGGGCAAAGTATCAGAAGAATGTCGAAGCATACCACAATAGGAGTGCCGGTATGAAGCCTAATATAGTACCCAATTGCATTAGATCGGAAAGCTACATGATCTCCTTTGAAGTGGAGGAAGAGAAGTTCCCTTTGTTTGGGAAGAAATATCAGCTGAAATTTGCAAATGACCCCTCTGCTGAGACTAATTGTTTAGTTCATTTTCCAAGCTTGATCAGGTTGGCTAGAGAAGCAGGTCTTGAGTATATCGAGATTCAAAATTTGACCGAATTCTTCGATGATCACAGAGCACAATTGGCAGATATGCTCATGAATTTTGGTCAAAACATTCTGGATCCCAGAGGGAGACTTCTTCCTCGATCATACGATGTACTGGGTCTCTACACAACATTCATATTTCAGAAACCAGATCCAGACATCACTCCACCAATTATGACCCCATTGTTGCCAGAAACTATTTATGATCATGAAGAGACAGAGTGGCTGGGGAATGTGTGGCGAGATGAAGAAAGAAGTGTACAGGCAGAGCATGTTTCTGGTCAGGTTCCAGTTCCTGTGCCTGTTCCGGTTCCTATTCCGGTTGCGGTTCCAGTTCCGGTTTCAGTTACCGTTCAGGTTCCTGTTGTGGTACCACTCACCGTCCCTGGGCTTGGCAAGATTAGTGAACAGAAGGGGATTTTGGGTCCTGGCCCTGCAGATTTACGTTTTCCGGAGGCTCTTTAG

Protein sequence

MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGIDESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFETEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISFEVEEEKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNLTEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPLLPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPVVVPLTVPGLGKISEQKGILGPGPADLRFPEAL*
Homology
BLAST of CSPI05G26580 vs. ExPASy Swiss-Prot
Match: Q5HZ60 (mRNA cap guanine-N7 methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g52210 PE=2 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 1.6e-131
Identity = 239/394 (60.66%), Postives = 283/394 (71.83%), Query Frame = 0

Query: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60
           MS  V  + E +HHRL DFAKTA+I IF+HPY TVC+LYC    + +KW+   I HYIGI
Sbjct: 1   MSGFVVSKPEQSHHRLFDFAKTAIINIFAHPYATVCELYCGGAPETDKWEAAPIGHYIGI 60

Query: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120
           D S SGI  +REAWESQ+K Y  EFFE DP  ++ E QL+ K E  D V C +HLQ+CFE
Sbjct: 61  DTS-SGISSVREAWESQRKNYDVEFFEADPSKDDFEIQLQKKLEQADLVSCWRHLQLCFE 120

Query: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180
           TEE ARRLL NV+ LLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRS G KPN+ PN IR
Sbjct: 121 TEESARRLLTNVACLLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRS-GAKPNVFPNYIR 180

Query: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNL 240
           SESYMI+FE+EEEKFPLFGK+YQLKF+ D ++E +CLVHFPSLIRLAREAGLE++EIQ+L
Sbjct: 181 SESYMITFELEEEKFPLFGKRYQLKFSGDNASEDHCLVHFPSLIRLAREAGLEFVEIQSL 240

Query: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300
           T+F+DD+RAQ A +LMN G N +DPRG+LLPR++D+LGLY TFIFQKPDPDI PP+ TP+
Sbjct: 241 TDFYDDNRAQFASLLMNAGPNFVDPRGKLLPRAFDLLGLYATFIFQKPDPDIEPPLTTPI 300

Query: 301 LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV 360
             E+  +H+E E                               +PV       +TV    
Sbjct: 301 PFESSNNHDERE-------------------------------LPV-------ITVITDA 354

Query: 361 VVPLTVP--GLGKISEQKGILGPGPADLRFPEAL 393
             P   P  GLGKI EQKGILGPGPADLRF EA+
Sbjct: 361 SAPAEDPSQGLGKIVEQKGILGPGPADLRFSEAI 354

BLAST of CSPI05G26580 vs. ExPASy Swiss-Prot
Match: Q6K833 (mRNA cap guanine-N7 methyltransferase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0780600 PE=2 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 1.7e-117
Identity = 197/302 (65.23%), Postives = 242/302 (80.13%), Query Frame = 0

Query: 13  HHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGIDESTSGIGQMRE 72
           HHRL +FAKTALIKIF+ PY TVCDLYC  G D +KW + QI HYIGID S SG+   RE
Sbjct: 6   HHRLYEFAKTALIKIFAFPYATVCDLYCDGGVDTDKWGDAQIGHYIGIDASASGVNDARE 65

Query: 73  AWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFETEERARRLLHNV 132
            WES+KK +T+EF E+DP  ++ E Q+++K    D VCC+QHLQ+CFE+EE A++LL+NV
Sbjct: 66  LWESRKKLFTSEFIELDPSADDFEAQMQEKGIQADIVCCMQHLQLCFESEEHAQKLLNNV 125

Query: 133 SALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISFEVEE 192
           S+LLKPGGYF GI PDSSTIW KYQKNVEA HN+  G+K   VPN IRSE+Y+I+FEVEE
Sbjct: 126 SSLLKPGGYFVGIIPDSSTIWTKYQKNVEASHNK--GLK--TVPNSIRSENYVITFEVEE 185

Query: 193 EKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNLTEFFDDHRAQLA 252
           EKFP FGKKYQLKFAN+   E +CLVHFPS +RLAREAGLEY+EIQNLTEF+DD+R Q A
Sbjct: 186 EKFPFFGKKYQLKFANESMFENHCLVHFPSFMRLAREAGLEYVEIQNLTEFYDDNRTQFA 245

Query: 253 DMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPLLPETIYDHEETE 312
            +L  +G +++DPRG+L+ RS+D+LGLY+TF+FQKPDPD  PPI+TP L +   D EE E
Sbjct: 246 PLLGGYGSSLVDPRGKLVARSFDILGLYSTFVFQKPDPDAMPPIVTPELHDPENDQEE-E 302

Query: 313 WL 315
           WL
Sbjct: 306 WL 302

BLAST of CSPI05G26580 vs. ExPASy Swiss-Prot
Match: Q6Z9U7 (mRNA cap guanine-N7 methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0180000 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 5.2e-21
Identity = 80/288 (27.78%), Postives = 131/288 (45.49%), Query Frame = 0

Query: 15  RLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGIDESTSGIGQMREAW 74
           +L ++ K+ LI++++HP   V DL C +G D  KWD+ ++ +Y+G+D +   I      +
Sbjct: 68  KLNNWIKSVLIQLYAHPGDCVLDLACGKGGDLIKWDKAKVGYYVGVDIAEGSIKDCMTRY 127

Query: 75  ESQKKAYTAEFFEVDP---CVENIETQLKD---KTETVDRVCCLQHLQMCFETEERARRL 134
                    + F       C +  E +L +   +    D   C   L   + TE RAR+ 
Sbjct: 128 NGDTDQQRRKKFSFPARLICADCYEARLDEHLYEDAPFDICSCQFALHYSWSTEARARQA 187

Query: 135 LHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISF 194
           L NVSALL+PGG F G  PD++ I  + ++        + GM+         +  Y ISF
Sbjct: 188 LANVSALLRPGGVFIGTMPDANVIIKRLRE--------TDGME-------FGNGVYWISF 247

Query: 195 --EVEEEKFPL---FGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNLTEF 254
             E  E+KFP    FG KY+     D       +V F     LA E  LE +  +N  EF
Sbjct: 248 GEEYAEKKFPASRPFGIKYKFHL-EDAVDCPEWVVPFHLFKLLAEEYDLELVLTKNFHEF 307

Query: 255 FDDH--RAQLADMLMNFGQ--NILDPRGRLLPRSYDVLGLYTTFIFQK 288
             ++  + + A+++   G   +    +  L    ++V  LY  F+ +K
Sbjct: 308 VHEYLQKPEFAELMRRLGALGDGRQDQSTLSQDEWEVAYLYLAFVLRK 339

BLAST of CSPI05G26580 vs. ExPASy Swiss-Prot
Match: Q9LHQ7 (mRNA cap guanine-N7 methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g20650 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.9e-19
Identity = 77/289 (26.64%), Postives = 131/289 (45.33%), Query Frame = 0

Query: 15  RLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGIDESTSGIGQMREAW 74
           +L ++ K+ LI++++ P   V DL C +G D  KWD+ +I +Y+GID +   I   R  +
Sbjct: 68  KLNNWIKSVLIQLYARPDDAVLDLACGKGGDLIKWDKARIGYYVGIDIAEGSIEDCRTRY 127

Query: 75  -------ESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFETEERARR 134
                  + +KK           C E    ++ ++    D   C   +   + TE RARR
Sbjct: 128 NGDADHHQRRKKFSFPSRLLCGDCFEVELDKILEEDAPFDICSCQFAMHYSWTTEARARR 187

Query: 135 LLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMIS 194
            L NVSALL+PGG F G  PD++ I  K ++        + G++       I +  Y I 
Sbjct: 188 ALANVSALLRPGGVFIGTMPDANVIIKKLRE--------AEGLE-------IGNSVYWIR 247

Query: 195 F--EVEEEKFPL---FGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNLTE 254
           F  E  ++KF     FG +Y      D       +V F     LA E  LE + ++N  E
Sbjct: 248 FGEEYSQKKFKSSSPFGIEYVFHL-EDAVDCPEWIVPFNVFKSLAEEYDLELVFVKNSHE 307

Query: 255 FFDDH--RAQLADMLMNFGQ--NILDPRGRLLPRSYDVLGLYTTFIFQK 288
           F  ++  + +  +++   G   +  + +  L    ++   LY +F+ +K
Sbjct: 308 FVHEYMKKPEFVELMRRLGALGDGSNDQSTLSADEWEAAYLYLSFVLRK 340

BLAST of CSPI05G26580 vs. ExPASy Swiss-Prot
Match: Q6CKI0 (mRNA cap guanine-N7 methyltransferase OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=ABD1 PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 7.0e-18
Identity = 86/285 (30.18%), Postives = 121/285 (42.46%), Query Frame = 0

Query: 21  KTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGIDESTSGIGQMREAWESQKK- 80
           K  LI  F+     V +L C +G D  K+    IS +IGID S + I +  + + S K  
Sbjct: 151 KYILIDKFTRAGDVVLELACGKGGDLRKYGAAGISQFIGIDISNASITEALKRYHSMKNL 210

Query: 81  AYTAEFFEVDPCVENIETQLKDKTE---TVDRVCCLQHLQMCFETEERARRLLHNVSALL 140
            Y       D   E++   ++   E     D V C   L   FETEE+ARR+L NV   L
Sbjct: 211 EYQVILITGDCFGESLGVAVESFPECRFPCDIVSCQFALHYAFETEEKARRMLLNVVKSL 270

Query: 141 KPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISFEVEE---- 200
           K GGYFFG  PDS  I  K  K  E+    S G           +  Y ++F   E    
Sbjct: 271 KIGGYFFGTIPDSEFIRYKMNKIPESVEKPSWG-----------NSIYKVTFSNNEYQKN 330

Query: 201 -EKFPL-FGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNLTEFFDDHRAQ 260
             +FP  FG+ Y     +        ++ F S   LA E G+E    +   EFF +   +
Sbjct: 331 GNEFPSPFGQMYTFWLEDAIDNVPEYVIPFESFRSLADEYGMELELQKGFNEFFVE---E 390

Query: 261 LADMLMNFGQNILDPRGRLLPRSYDVLGL--------YTTFIFQK 288
           + + +  F   + +   R   R Y V G+        YTTF F+K
Sbjct: 391 IPNWVNRFSPKMREGLKRSDGR-YGVEGVEKEPAAYFYTTFAFRK 420

BLAST of CSPI05G26580 vs. ExPASy TrEMBL
Match: A0A0A0KTG9 (mRNA (guanine-N(7)-)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G616340 PE=3 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 1.3e-229
Identity = 390/392 (99.49%), Postives = 391/392 (99.74%), Query Frame = 0

Query: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60
           MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI
Sbjct: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60

Query: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120
           DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE
Sbjct: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120

Query: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180
           TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR
Sbjct: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180

Query: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNL 240
           SESYMISFEVEEEKFPLFGKKYQLKFANDPSAET+CLVHFPS IRLAREAGLEYIEIQNL
Sbjct: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETHCLVHFPSFIRLAREAGLEYIEIQNL 240

Query: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300
           TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL
Sbjct: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300

Query: 301 LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV 360
           LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV
Sbjct: 301 LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV 360

Query: 361 VVPLTVPGLGKISEQKGILGPGPADLRFPEAL 393
           VVPLTVPGLGKISEQKGILGPGPADLRFPEAL
Sbjct: 361 VVPLTVPGLGKISEQKGILGPGPADLRFPEAL 392

BLAST of CSPI05G26580 vs. ExPASy TrEMBL
Match: A0A1S3BFX8 (mRNA (guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103489414 PE=3 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 7.6e-225
Identity = 382/392 (97.45%), Postives = 384/392 (97.96%), Query Frame = 0

Query: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60
           MSQ+VPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDE QISHYIGI
Sbjct: 1   MSQIVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDEAQISHYIGI 60

Query: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120
           DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTET DRVCCLQHLQMCFE
Sbjct: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETADRVCCLQHLQMCFE 120

Query: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180
           TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR
Sbjct: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180

Query: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNL 240
           SESYMISFEVEEEKFPLFGKKYQLKFANDPSAET+CLVHFPS IRLAREAGLEYIEIQNL
Sbjct: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETHCLVHFPSFIRLAREAGLEYIEIQNL 240

Query: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300
           TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL
Sbjct: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300

Query: 301 LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV 360
           LPE  YDHEE EW GNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTV VPV
Sbjct: 301 LPEISYDHEEIEWQGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVPVPV 360

Query: 361 VVPLTVPGLGKISEQKGILGPGPADLRFPEAL 393
           VVPLTVPGLGKISEQKGILGPGPADLRFPEAL
Sbjct: 361 VVPLTVPGLGKISEQKGILGPGPADLRFPEAL 392

BLAST of CSPI05G26580 vs. ExPASy TrEMBL
Match: A0A5A7STY7 (mRNA cap guanine-N7 methyltransferase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G006930 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.4e-202
Identity = 349/359 (97.21%), Postives = 351/359 (97.77%), Query Frame = 0

Query: 34  TVCDLYCARGADAEKWDETQISHYIGIDESTSGIGQMREAWESQKKAYTAEFFEVDPCVE 93
           +VCDLYCARGADAEKWDE QISHYIGIDESTSGIGQMREAWESQKKAYTAEFFEVDPCVE
Sbjct: 332 SVCDLYCARGADAEKWDEAQISHYIGIDESTSGIGQMREAWESQKKAYTAEFFEVDPCVE 391

Query: 94  NIETQLKDKTETVDRVCCLQHLQMCFETEERARRLLHNVSALLKPGGYFFGITPDSSTIW 153
           NIETQLKDKTET DRVCCLQHLQMCFETEERARRLLHNVSALLKPGGYFFGITPDSSTIW
Sbjct: 392 NIETQLKDKTETADRVCCLQHLQMCFETEERARRLLHNVSALLKPGGYFFGITPDSSTIW 451

Query: 154 AKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISFEVEEEKFPLFGKKYQLKFANDPSAE 213
           AKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISFEVEEEKFPLFGKKYQLKFANDPSAE
Sbjct: 452 AKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISFEVEEEKFPLFGKKYQLKFANDPSAE 511

Query: 214 TNCLVHFPSLIRLAREAGLEYIEIQNLTEFFDDHRAQLADMLMNFGQNILDPRGRLLPRS 273
           T+CLVHFPS IRLAREAGLEYIEIQNLTEFFDDHRAQLADMLMNFGQNILDPRGRLLPRS
Sbjct: 512 THCLVHFPSFIRLAREAGLEYIEIQNLTEFFDDHRAQLADMLMNFGQNILDPRGRLLPRS 571

Query: 274 YDVLGLYTTFIFQKPDPDITPPIMTPLLPETIYDHEETEWLGNVWRDEERSVQAEHVSGQ 333
           YDVLGLYTTFIFQKPDPDITPPIMTPLLPE  YDHEE EW GNVWRDEERSVQAEHVSGQ
Sbjct: 572 YDVLGLYTTFIFQKPDPDITPPIMTPLLPEISYDHEEIEWQGNVWRDEERSVQAEHVSGQ 631

Query: 334 VPVPVPVPVPIPVAVPVPVSVTVQVPVVVPLTVPGLGKISEQKGILGPGPADLRFPEAL 393
           VPVPVPVPVPIPVAVPVPVSVTV VPVVVPLTVPGLGKISEQKGILGPGPADLRFPEAL
Sbjct: 632 VPVPVPVPVPIPVAVPVPVSVTVPVPVVVPLTVPGLGKISEQKGILGPGPADLRFPEAL 690

BLAST of CSPI05G26580 vs. ExPASy TrEMBL
Match: A0A6J1HXN3 (mRNA (guanine-N(7)-)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111467776 PE=3 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 7.6e-201
Identity = 347/394 (88.07%), Postives = 358/394 (90.86%), Query Frame = 0

Query: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60
           MSQLVP R E THHRL DFAK ALIKIF+HPYVTVCDLYCARG DAEKWDE Q SHYIGI
Sbjct: 1   MSQLVPSRTESTHHRLYDFAKAALIKIFAHPYVTVCDLYCARGTDAEKWDEAQFSHYIGI 60

Query: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120
           D STSGIGQMREAWESQ+KAYTAEFFEVDPCVENIETQ+KDK ET D VCCLQHLQMCFE
Sbjct: 61  DASTSGIGQMREAWESQRKAYTAEFFEVDPCVENIETQMKDKAETADLVCCLQHLQMCFE 120

Query: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180
           TEERARRLLHNVS LLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSA MKPNIVPNCIR
Sbjct: 121 TEERARRLLHNVSLLLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSASMKPNIVPNCIR 180

Query: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNL 240
           SESYMI+FEVEEEKFPLFGKKYQLKFANDPSAET+CLVHFPS IRLAREAGLEYIEIQNL
Sbjct: 181 SESYMITFEVEEEKFPLFGKKYQLKFANDPSAETHCLVHFPSFIRLAREAGLEYIEIQNL 240

Query: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300
           TEF+DDHRAQ A MLMN G N+LDPRGRLLPRSYDVLGLYTTFIFQKPDP ITPPIMTPL
Sbjct: 241 TEFYDDHRAQFAGMLMNCGPNLLDPRGRLLPRSYDVLGLYTTFIFQKPDPTITPPIMTPL 300

Query: 301 LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVT--VQV 360
           L ET YDHEE EW G+ WRDEERSVQAEHV G VPVP P PVP+PV VPVPVSV   V V
Sbjct: 301 LLETSYDHEEREWQGSGWRDEERSVQAEHVPGPVPVPAPAPVPVPVPVPVPVSVAVPVPV 360

Query: 361 PVVVPLTVPGLGKISEQKGILGPGPADLRFPEAL 393
           PVV+P+ V GLGKISEQKGILGPGPADLRFPEAL
Sbjct: 361 PVVMPIPVSGLGKISEQKGILGPGPADLRFPEAL 394

BLAST of CSPI05G26580 vs. ExPASy TrEMBL
Match: A0A5D3CCK5 (GABA transporter 1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002180 PE=3 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 8.4e-200
Identity = 349/371 (94.07%), Postives = 351/371 (94.61%), Query Frame = 0

Query: 34  TVCDLYCARGADAEKWDETQISHYIGIDESTSGIGQMREAWESQKKAYTAEFFEVDPCVE 93
           +VCDLYCARGADAEKWDE QISHYIGIDESTSGIGQMREAWESQKKAYTAEFFEVDPCVE
Sbjct: 332 SVCDLYCARGADAEKWDEAQISHYIGIDESTSGIGQMREAWESQKKAYTAEFFEVDPCVE 391

Query: 94  NIETQLKDKTETVDRVCCLQHLQMCFETEERARRLLHNVSALLKPGGYFFGITPDSSTIW 153
           NIETQLKDKTET DRVCCLQHLQMCFETEERARRLLHNVSALLKPGGYFFGITPDSSTIW
Sbjct: 392 NIETQLKDKTETADRVCCLQHLQMCFETEERARRLLHNVSALLKPGGYFFGITPDSSTIW 451

Query: 154 AKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISFEVEEEKFPLFGKKYQLKFANDPSAE 213
           AKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISFEVEEEKFPLFGKKYQLKFANDPSAE
Sbjct: 452 AKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISFEVEEEKFPLFGKKYQLKFANDPSAE 511

Query: 214 TNCLVHFPSLIRLAREAGLEYIEIQNLTEFFDDHRAQLADMLMNFGQNILDPRGRLLPRS 273
           T+CLVHFPS IRLAREAGLEYIEIQNLTEFFDDHRAQLADMLMNFGQNILDPRGRLLPRS
Sbjct: 512 THCLVHFPSFIRLAREAGLEYIEIQNLTEFFDDHRAQLADMLMNFGQNILDPRGRLLPRS 571

Query: 274 YDVLGLYTTFIFQKPDPDITPPIMTPLLPETIYDHEET------------EWLGNVWRDE 333
           YDVLGLYTTFIFQKPDPDITPPIMTPLLPE  YDHEE             EW GNVWRDE
Sbjct: 572 YDVLGLYTTFIFQKPDPDITPPIMTPLLPEISYDHEEASFCLQKFFTNIIEWQGNVWRDE 631

Query: 334 ERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPVVVPLTVPGLGKISEQKGILGP 393
           ERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTV VPVVVPLTVPGLGKISEQKGILGP
Sbjct: 632 ERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVPVPVVVPLTVPGLGKISEQKGILGP 691

BLAST of CSPI05G26580 vs. NCBI nr
Match: XP_011655847.1 (mRNA cap guanine-N7 methyltransferase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 805.1 bits (2078), Expect = 2.8e-229
Identity = 390/392 (99.49%), Postives = 391/392 (99.74%), Query Frame = 0

Query: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60
           MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI
Sbjct: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60

Query: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120
           DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE
Sbjct: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120

Query: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180
           TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR
Sbjct: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180

Query: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNL 240
           SESYMISFEVEEEKFPLFGKKYQLKFANDPSAET+CLVHFPS IRLAREAGLEYIEIQNL
Sbjct: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETHCLVHFPSFIRLAREAGLEYIEIQNL 240

Query: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300
           TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL
Sbjct: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300

Query: 301 LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV 360
           LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV
Sbjct: 301 LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV 360

Query: 361 VVPLTVPGLGKISEQKGILGPGPADLRFPEAL 393
           VVPLTVPGLGKISEQKGILGPGPADLRFPEAL
Sbjct: 361 VVPLTVPGLGKISEQKGILGPGPADLRFPEAL 392

BLAST of CSPI05G26580 vs. NCBI nr
Match: KAE8648836.1 (hypothetical protein Csa_008498 [Cucumis sativus])

HSP 1 Score: 801.6 bits (2069), Expect = 3.1e-228
Identity = 388/392 (98.98%), Postives = 390/392 (99.49%), Query Frame = 0

Query: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60
           MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI
Sbjct: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60

Query: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120
           DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE
Sbjct: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120

Query: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180
           TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR
Sbjct: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180

Query: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNL 240
           SESYMISFEVEEEKFPLFGKKYQLKFANDPSAET+CLVHFPS IRLAREAGLEYIEIQNL
Sbjct: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETHCLVHFPSFIRLAREAGLEYIEIQNL 240

Query: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300
           TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL
Sbjct: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300

Query: 301 LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV 360
           LPETIYDHEE +WLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV
Sbjct: 301 LPETIYDHEEAKWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV 360

Query: 361 VVPLTVPGLGKISEQKGILGPGPADLRFPEAL 393
           VVPLTVPGLGKISEQKGILGPGPADLRFPEAL
Sbjct: 361 VVPLTVPGLGKISEQKGILGPGPADLRFPEAL 392

BLAST of CSPI05G26580 vs. NCBI nr
Match: XP_008446791.1 (PREDICTED: mRNA cap guanine-N7 methyltransferase 2 [Cucumis melo])

HSP 1 Score: 789.3 bits (2037), Expect = 1.6e-224
Identity = 382/392 (97.45%), Postives = 384/392 (97.96%), Query Frame = 0

Query: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60
           MSQ+VPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDE QISHYIGI
Sbjct: 1   MSQIVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDEAQISHYIGI 60

Query: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120
           DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTET DRVCCLQHLQMCFE
Sbjct: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETADRVCCLQHLQMCFE 120

Query: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180
           TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR
Sbjct: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180

Query: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNL 240
           SESYMISFEVEEEKFPLFGKKYQLKFANDPSAET+CLVHFPS IRLAREAGLEYIEIQNL
Sbjct: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETHCLVHFPSFIRLAREAGLEYIEIQNL 240

Query: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300
           TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL
Sbjct: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300

Query: 301 LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV 360
           LPE  YDHEE EW GNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTV VPV
Sbjct: 301 LPEISYDHEEIEWQGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVPVPV 360

Query: 361 VVPLTVPGLGKISEQKGILGPGPADLRFPEAL 393
           VVPLTVPGLGKISEQKGILGPGPADLRFPEAL
Sbjct: 361 VVPLTVPGLGKISEQKGILGPGPADLRFPEAL 392

BLAST of CSPI05G26580 vs. NCBI nr
Match: XP_038891845.1 (mRNA cap guanine-N7 methyltransferase 2 [Benincasa hispida])

HSP 1 Score: 750.7 bits (1937), Expect = 6.2e-213
Identity = 362/392 (92.35%), Postives = 374/392 (95.41%), Query Frame = 0

Query: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60
           MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDE QISHYIGI
Sbjct: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDEAQISHYIGI 60

Query: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120
           DES+SGIGQMREAWESQ+K+YTAEFFEVDPCVENIE  LKDKTE  D V CLQHLQMCFE
Sbjct: 61  DESSSGIGQMREAWESQRKSYTAEFFEVDPCVENIEAHLKDKTEIADLVSCLQHLQMCFE 120

Query: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180
           TEERA+RLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR
Sbjct: 121 TEERAQRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180

Query: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNL 240
           SESYMI+FEVEEEKFPLFGKKYQLKFAND SAET+CLVHFPS IRLAREAGLEYIEIQNL
Sbjct: 181 SESYMITFEVEEEKFPLFGKKYQLKFANDLSAETHCLVHFPSFIRLAREAGLEYIEIQNL 240

Query: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300
           TEFFDDHRAQ A MLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL
Sbjct: 241 TEFFDDHRAQFAGMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300

Query: 301 LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV 360
           LPET YDHEE EW G+VWRDEERSVQ +HVSG VPV VPVPVP+PVAVPVPVSVTV VPV
Sbjct: 301 LPETSYDHEEIEWQGSVWRDEERSVQPDHVSGPVPVLVPVPVPVPVAVPVPVSVTVPVPV 360

Query: 361 VVPLTVPGLGKISEQKGILGPGPADLRFPEAL 393
           VVP+++PGLGKISEQKGILGPGPADLRFPEAL
Sbjct: 361 VVPISIPGLGKISEQKGILGPGPADLRFPEAL 392

BLAST of CSPI05G26580 vs. NCBI nr
Match: KAA0034674.1 (mRNA cap guanine-N7 methyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 714.5 bits (1843), Expect = 4.9e-202
Identity = 349/359 (97.21%), Postives = 351/359 (97.77%), Query Frame = 0

Query: 34  TVCDLYCARGADAEKWDETQISHYIGIDESTSGIGQMREAWESQKKAYTAEFFEVDPCVE 93
           +VCDLYCARGADAEKWDE QISHYIGIDESTSGIGQMREAWESQKKAYTAEFFEVDPCVE
Sbjct: 332 SVCDLYCARGADAEKWDEAQISHYIGIDESTSGIGQMREAWESQKKAYTAEFFEVDPCVE 391

Query: 94  NIETQLKDKTETVDRVCCLQHLQMCFETEERARRLLHNVSALLKPGGYFFGITPDSSTIW 153
           NIETQLKDKTET DRVCCLQHLQMCFETEERARRLLHNVSALLKPGGYFFGITPDSSTIW
Sbjct: 392 NIETQLKDKTETADRVCCLQHLQMCFETEERARRLLHNVSALLKPGGYFFGITPDSSTIW 451

Query: 154 AKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISFEVEEEKFPLFGKKYQLKFANDPSAE 213
           AKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISFEVEEEKFPLFGKKYQLKFANDPSAE
Sbjct: 452 AKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMISFEVEEEKFPLFGKKYQLKFANDPSAE 511

Query: 214 TNCLVHFPSLIRLAREAGLEYIEIQNLTEFFDDHRAQLADMLMNFGQNILDPRGRLLPRS 273
           T+CLVHFPS IRLAREAGLEYIEIQNLTEFFDDHRAQLADMLMNFGQNILDPRGRLLPRS
Sbjct: 512 THCLVHFPSFIRLAREAGLEYIEIQNLTEFFDDHRAQLADMLMNFGQNILDPRGRLLPRS 571

Query: 274 YDVLGLYTTFIFQKPDPDITPPIMTPLLPETIYDHEETEWLGNVWRDEERSVQAEHVSGQ 333
           YDVLGLYTTFIFQKPDPDITPPIMTPLLPE  YDHEE EW GNVWRDEERSVQAEHVSGQ
Sbjct: 572 YDVLGLYTTFIFQKPDPDITPPIMTPLLPEISYDHEEIEWQGNVWRDEERSVQAEHVSGQ 631

Query: 334 VPVPVPVPVPIPVAVPVPVSVTVQVPVVVPLTVPGLGKISEQKGILGPGPADLRFPEAL 393
           VPVPVPVPVPIPVAVPVPVSVTV VPVVVPLTVPGLGKISEQKGILGPGPADLRFPEAL
Sbjct: 632 VPVPVPVPVPIPVAVPVPVSVTVPVPVVVPLTVPGLGKISEQKGILGPGPADLRFPEAL 690

BLAST of CSPI05G26580 vs. TAIR 10
Match: AT3G52210.3 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 472.2 bits (1214), Expect = 4.0e-133
Identity = 237/394 (60.15%), Postives = 284/394 (72.08%), Query Frame = 0

Query: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60
           MS  V  + E +HHRL DFAKTA+I IF+HPY TVC+LYC    + +KW+   I HYIGI
Sbjct: 1   MSGFVVSKPEQSHHRLFDFAKTAIINIFAHPYATVCELYCGGAPETDKWEAAPIGHYIGI 60

Query: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120
            +++SGI  +REAWESQ+K Y  EFFE DP  ++ E QL+ K E  D V C +HLQ+CFE
Sbjct: 61  VDTSSGISSVREAWESQRKNYDVEFFEADPSKDDFEIQLQKKLEQADLVSCWRHLQLCFE 120

Query: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180
           TEE ARRLL NV+ LLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRS G KPN+ PN IR
Sbjct: 121 TEESARRLLTNVACLLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRS-GAKPNVFPNYIR 180

Query: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNL 240
           SESYMI+FE+EEEKFPLFGK+YQLKF+ D ++E +CLVHFPSLIRLAREAGLE++EIQ+L
Sbjct: 181 SESYMITFELEEEKFPLFGKRYQLKFSGDNASEDHCLVHFPSLIRLAREAGLEFVEIQSL 240

Query: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300
           T+F+DD+RAQ A +LMN G N +DPRG+LLPR++D+LGLY TFIFQKPDPDI PP+ TP+
Sbjct: 241 TDFYDDNRAQFASLLMNAGPNFVDPRGKLLPRAFDLLGLYATFIFQKPDPDIEPPLTTPI 300

Query: 301 LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV 360
             E+  +H+E E                               +PV       +TV    
Sbjct: 301 PFESSNNHDERE-------------------------------LPV-------ITVITDA 355

Query: 361 VVPLTVP--GLGKISEQKGILGPGPADLRFPEAL 393
             P   P  GLGKI EQKGILGPGPADLRF EA+
Sbjct: 361 SAPAEDPSQGLGKIVEQKGILGPGPADLRFSEAI 355

BLAST of CSPI05G26580 vs. TAIR 10
Match: AT3G52210.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 470.7 bits (1210), Expect = 1.1e-132
Identity = 239/394 (60.66%), Postives = 283/394 (71.83%), Query Frame = 0

Query: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60
           MS  V  + E +HHRL DFAKTA+I IF+HPY TVC+LYC    + +KW+   I HYIGI
Sbjct: 1   MSGFVVSKPEQSHHRLFDFAKTAIINIFAHPYATVCELYCGGAPETDKWEAAPIGHYIGI 60

Query: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120
           D S SGI  +REAWESQ+K Y  EFFE DP  ++ E QL+ K E  D V C +HLQ+CFE
Sbjct: 61  DTS-SGISSVREAWESQRKNYDVEFFEADPSKDDFEIQLQKKLEQADLVSCWRHLQLCFE 120

Query: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180
           TEE ARRLL NV+ LLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRS G KPN+ PN IR
Sbjct: 121 TEESARRLLTNVACLLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRS-GAKPNVFPNYIR 180

Query: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNL 240
           SESYMI+FE+EEEKFPLFGK+YQLKF+ D ++E +CLVHFPSLIRLAREAGLE++EIQ+L
Sbjct: 181 SESYMITFELEEEKFPLFGKRYQLKFSGDNASEDHCLVHFPSLIRLAREAGLEFVEIQSL 240

Query: 241 TEFFDDHRAQLADMLMNFGQNILDPRGRLLPRSYDVLGLYTTFIFQKPDPDITPPIMTPL 300
           T+F+DD+RAQ A +LMN G N +DPRG+LLPR++D+LGLY TFIFQKPDPDI PP+ TP+
Sbjct: 241 TDFYDDNRAQFASLLMNAGPNFVDPRGKLLPRAFDLLGLYATFIFQKPDPDIEPPLTTPI 300

Query: 301 LPETIYDHEETEWLGNVWRDEERSVQAEHVSGQVPVPVPVPVPIPVAVPVPVSVTVQVPV 360
             E+  +H+E E                               +PV       +TV    
Sbjct: 301 PFESSNNHDERE-------------------------------LPV-------ITVITDA 354

Query: 361 VVPLTVP--GLGKISEQKGILGPGPADLRFPEAL 393
             P   P  GLGKI EQKGILGPGPADLRF EA+
Sbjct: 361 SAPAEDPSQGLGKIVEQKGILGPGPADLRFSEAI 354

BLAST of CSPI05G26580 vs. TAIR 10
Match: AT3G52210.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 362.1 bits (928), Expect = 5.8e-100
Identity = 171/250 (68.40%), Postives = 201/250 (80.40%), Query Frame = 0

Query: 1   MSQLVPPRGEWTHHRLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGI 60
           MS  V  + E +HHRL DFAKTA+I IF+HPY TVC+LYC    + +KW+   I HYIGI
Sbjct: 1   MSGFVVSKPEQSHHRLFDFAKTAIINIFAHPYATVCELYCGGAPETDKWEAAPIGHYIGI 60

Query: 61  DESTSGIGQMREAWESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFE 120
           D S SGI  +REAWESQ+K Y  EFFE DP  ++ E QL+ K E  D V C +HLQ+CFE
Sbjct: 61  DTS-SGISSVREAWESQRKNYDVEFFEADPSKDDFEIQLQKKLEQADLVSCWRHLQLCFE 120

Query: 121 TEERARRLLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIR 180
           TEE ARRLL NV+ LLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRS G KPN+ PN IR
Sbjct: 121 TEESARRLLTNVACLLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRS-GAKPNVFPNYIR 180

Query: 181 SESYMISFEVEEEKFPLFGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNL 240
           SESYMI+FE+EEEKFPLFGK+YQLKF+ D ++E +CLVHFPSLIRLAREAGLE++EIQ+L
Sbjct: 181 SESYMITFELEEEKFPLFGKRYQLKFSGDNASEDHCLVHFPSLIRLAREAGLEFVEIQSL 240

Query: 241 TEFFDDHRAQ 251
           T+F+DD+  Q
Sbjct: 241 TDFYDDNSLQ 248

BLAST of CSPI05G26580 vs. TAIR 10
Match: AT3G20650.1 (mRNA capping enzyme family protein )

HSP 1 Score: 97.1 bits (240), Expect = 3.5e-20
Identity = 77/289 (26.64%), Postives = 131/289 (45.33%), Query Frame = 0

Query: 15  RLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGIDESTSGIGQMREAW 74
           +L ++ K+ LI++++ P   V DL C +G D  KWD+ +I +Y+GID +   I   R  +
Sbjct: 68  KLNNWIKSVLIQLYARPDDAVLDLACGKGGDLIKWDKARIGYYVGIDIAEGSIEDCRTRY 127

Query: 75  -------ESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFETEERARR 134
                  + +KK           C E    ++ ++    D   C   +   + TE RARR
Sbjct: 128 NGDADHHQRRKKFSFPSRLLCGDCFEVELDKILEEDAPFDICSCQFAMHYSWTTEARARR 187

Query: 135 LLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMIS 194
            L NVSALL+PGG F G  PD++ I  K ++        + G++       I +  Y I 
Sbjct: 188 ALANVSALLRPGGVFIGTMPDANVIIKKLRE--------AEGLE-------IGNSVYWIR 247

Query: 195 F--EVEEEKFPL---FGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNLTE 254
           F  E  ++KF     FG +Y      D       +V F     LA E  LE + ++N  E
Sbjct: 248 FGEEYSQKKFKSSSPFGIEYVFHL-EDAVDCPEWIVPFNVFKSLAEEYDLELVFVKNSHE 307

Query: 255 FFDDH--RAQLADMLMNFGQ--NILDPRGRLLPRSYDVLGLYTTFIFQK 288
           F  ++  + +  +++   G   +  + +  L    ++   LY +F+ +K
Sbjct: 308 FVHEYMKKPEFVELMRRLGALGDGSNDQSTLSADEWEAAYLYLSFVLRK 340

BLAST of CSPI05G26580 vs. TAIR 10
Match: AT3G20650.2 (mRNA capping enzyme family protein )

HSP 1 Score: 95.9 bits (237), Expect = 7.7e-20
Identity = 77/288 (26.74%), Postives = 130/288 (45.14%), Query Frame = 0

Query: 15  RLCDFAKTALIKIFSHPYVTVCDLYCARGADAEKWDETQISHYIGIDESTSGIGQMREAW 74
           +L ++ K+ LI++++ P   V DL C +G D  KWD+ +I +Y+GID +   I   R  +
Sbjct: 68  KLNNWIKSVLIQLYARPDDAVLDLACGKGGDLIKWDKARIGYYVGIDIAEGSIEDCRTRY 127

Query: 75  -------ESQKKAYTAEFFEVDPCVENIETQLKDKTETVDRVCCLQHLQMCFETEERARR 134
                  + +KK           C E    ++ ++    D   C   +   + TE RARR
Sbjct: 128 NGDADHHQRRKKFSFPSRLLCGDCFEVELDKILEEDAPFDICSCQFAMHYSWTTEARARR 187

Query: 135 LLHNVSALLKPGGYFFGITPDSSTIWAKYQKNVEAYHNRSAGMKPNIVPNCIRSESYMIS 194
            L NVSALL+PGG F G  PD++ I  K ++        + G++       I +  Y I 
Sbjct: 188 ALANVSALLRPGGVFIGTMPDANVIIKKLRE--------AEGLE-------IGNSVYWIR 247

Query: 195 F-EVEEEKFPL---FGKKYQLKFANDPSAETNCLVHFPSLIRLAREAGLEYIEIQNLTEF 254
           F E   +KF     FG +Y      D       +V F     LA E  LE + ++N  EF
Sbjct: 248 FGEEYSQKFKSSSPFGIEYVFHL-EDAVDCPEWIVPFNVFKSLAEEYDLELVFVKNSHEF 307

Query: 255 FDDH--RAQLADMLMNFGQ--NILDPRGRLLPRSYDVLGLYTTFIFQK 288
             ++  + +  +++   G   +  + +  L    ++   LY +F+ +K
Sbjct: 308 VHEYMKKPEFVELMRRLGALGDGSNDQSTLSADEWEAAYLYLSFVLRK 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5HZ601.6e-13160.66mRNA cap guanine-N7 methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g5... [more]
Q6K8331.7e-11765.23mRNA cap guanine-N7 methyltransferase 2 OS=Oryza sativa subsp. japonica OX=39947... [more]
Q6Z9U75.2e-2127.78mRNA cap guanine-N7 methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947... [more]
Q9LHQ74.9e-1926.64mRNA cap guanine-N7 methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g2... [more]
Q6CKI07.0e-1830.18mRNA cap guanine-N7 methyltransferase OS=Kluyveromyces lactis (strain ATCC 8585 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KTG91.3e-22999.49mRNA (guanine-N(7)-)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G61634... [more]
A0A1S3BFX87.6e-22597.45mRNA (guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103489414 P... [more]
A0A5A7STY72.4e-20297.21mRNA cap guanine-N7 methyltransferase 2 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1HXN37.6e-20188.07mRNA (guanine-N(7)-)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1114677... [more]
A0A5D3CCK58.4e-20094.07GABA transporter 1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
XP_011655847.12.8e-22999.49mRNA cap guanine-N7 methyltransferase 2 isoform X1 [Cucumis sativus][more]
KAE8648836.13.1e-22898.98hypothetical protein Csa_008498 [Cucumis sativus][more]
XP_008446791.11.6e-22497.45PREDICTED: mRNA cap guanine-N7 methyltransferase 2 [Cucumis melo][more]
XP_038891845.16.2e-21392.35mRNA cap guanine-N7 methyltransferase 2 [Benincasa hispida][more]
KAA0034674.14.9e-20297.21mRNA cap guanine-N7 methyltransferase 2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT3G52210.34.0e-13360.15S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G52210.11.1e-13260.66S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G52210.25.8e-10068.40S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G20650.13.5e-2026.64mRNA capping enzyme family protein [more]
AT3G20650.27.7e-2026.74mRNA capping enzyme family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 15..288
e-value: 3.8E-58
score: 199.0
NoneNo IPR availablePANTHERPTHR12189:SF3MRNA CAP GUANINE-N7 METHYLTRANSFERASE 2coord: 12..316
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 34..143
e-value: 1.02962E-6
score: 45.1135
IPR004971mRNA (guanine-N(7))-methyltransferase domainPFAMPF03291Pox_MCELcoord: 17..257
e-value: 4.3E-33
score: 114.8
IPR004971mRNA (guanine-N(7))-methyltransferase domainPROSITEPS51562RNA_CAP0_MTcoord: 1..295
score: 57.001083
IPR039753mRNA cap guanine-N7 methyltransferasePANTHERPTHR12189MRNA GUANINE-7- METHYLTRANSFERASEcoord: 12..316
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 28..288

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G26580.1CSPI05G26580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006370 7-methylguanosine mRNA capping
biological_process GO:0006865 amino acid transport
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0106005 RNA 5'-cap (guanine-N7)-methylation
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005845 mRNA cap binding complex
cellular_component GO:0005634 nucleus
molecular_function GO:0004482 mRNA (guanine-N7-)-methyltransferase activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0015293 symporter activity